(2008) Morphometric and Population Genetic Analyses
Botanical Journal of the Linnean Society, 2008, 157, 687–711. With 11 figures Morphometric and population genetic analyses elucidate the origin, evolutionary significance and conservation implications of Orchis ¥angusticruris (O. purpurea ¥ O. simia), a hybrid orchid new to Britain RICHARD M. BATEMAN*, RHIAN J. SMITH and MICHAEL F. FAY Jodrell Laboratory, Royal Botanic Gardens Kew, Richmond, Surrey TW9 3DS, UK Received 16 January 2008; accepted for publication 17 March 2008 We report the first confirmed occurrence in Britain of Orchis ¥ angusticruris Franch. ex Rouy, a hybrid between two closely related orchid species of anthropomorphic Orchis (O. purpurea Huds. ¥ O. simia Lam.) that hybridize frequently in Continental Europe. Seven individual hybrids, most likely F1 plants representing a single interspe- cific pollination event, first flowered with both parents in May 2006 at a nature reserve in the Chiltern Hills near Goring, Oxfordshire. Univariate and multivariate morphometric analyses (43 characters plus 12 indices), internal transcribed spacer sequencing, plastid microsatellites and amplified fragment length polymorphism (AFLP) analyses together readily separate the parents and confirm that O. purpurea was the ovule parent and O. simia the pollen parent, presumably reflecting the greater frequency and/or later flowering period of the latter at the site. This study reinforces a more general observation that, in most orchids, the ovule parent contributes substantially more to the hybrid phenotype than does the pollen parent, perhaps reflecting cytoplasmic inheritance. In contrast, the hybrids are placed closer to O. simia than to O. purpurea in the AFLP tree. Apparently recent arrivals, the few O. purpurea plants at Goring contrast genetically with the two other small populations of this species known in the Chilterns, but rather are consistent with relatively uncommon Continental populations.
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