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And Postsynaptic Dopamine D2 Receptors in Rat Brain Striatum

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And Postsynaptic Dopamine D2 Receptors in Rat Brain Striatum Functional responses of pre- and postsynaptic dopamine D2 receptors in rat brain striatum Guofen Ma Autonomous University of Barcelona, Spain ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! Functional responses of pre- and postsynaptic dopamine D2 receptors in rat brain striatum Memory by Guofen Ma, Master of Biochemistry and Molecular Biology, for the degree of Doctor from the Autonomous University of Barcelona. The work was done under the co-direction of Ph.D. Jordi Ortiz de Pablo and Josefa Sabria Pau at the Autonomous University of Barcelona, Spain Jordi Ortiz de Pablo Josefa Sabria Pau Guofen Ma Autonomous University of Barcelona, Spain ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! Abbreviations AC adenylyl cyclase AMPA Į-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptors ANOVA ANalysis Of VAriance ARI aripiprazole ATPase adenosine triphosphatase BSA bovine serum albumin CaMK calcium/calmodulin dependent kinase cAMP cyclic adenosine 3',5'-monophosphate CGP CGP 54626, antagonist of GABAB receptor CNS central nervous system CRE cAMP regulated element CREB CRE-binding protein D1R, D2R dopamine D1 receptor, dopamine D2 receptor DAG diacylglycerol DARPP-32 dopamine and cAMP-regulated phosphoprotein of 32kDa DAT dopamine transporter DNA deoxyribonucleic acid DNS donkey serum DTT dithiothreitol ERK extracellular signal-regulated kinase EPSC excitatory postsynaptic currents fEPSP field excitatory postsynaptic potential FMR1 fragile X mental retardation 1 FR fixed ratio FRET Förster resonance energy transfer GABA Ȗ -aminobutyric acid GADPH glyceraldehyde 3-phosphate dehydrogenase G-protein guanine protein GDP guanosine diphosphate GEF guanine exchange factor GP glopus pallidus GPCR guanine nucleotide binding protein receptors GRK GPCR kinase GSK3 glycogen synthase kinase 3 GTP guanosine triphosphate HFS high frequency stimulation HRP horseradish peroxidase IP 3 inositol (1,3,4) triphosphate LFS low frequency stimulation LTD long-term depression LTP long-term potentiation MAP mitogen activated protein MAPK MAP kinase mEPSC miniature excitatory postsynaptic current mGluR metabotropic glutamate receptor MAO monoamine oxidase MEK MAPK kinase MSN medium spiny neurons OKA akadaic acid, an PP2A inhibitor O/N over night PAGE polyacrylamide gel electrophoresis PAM positive allostoric modulator PBS phosphate buffered saline PET positron emission tomography PIP 2 phosphatidylinositol diphosphate PI3K phosphatidylinositol 3-kinases PKA protein kinase A PKC protein kinase C PLA 2 phospholipase A 2 PLC phospholipase C PP1 protein phosphatase 1 PP2A protein phosphatase 2A PPR paired pulse ratio QUIN quinpirole, agonist of D2-like receptors RT room temperature RACL raclopride, antagonist of D2 receptors SDS sodium dodecyl sulfate SN substantia nigra SP substance P STN subthalamic nucleus SUL sulpiride, antagonist of D2-like receptor TBS tris buffered saline TB tris buffer without saline TRP transient receptor potential TH tyrosine hydroxylase VTA ventral tegmental area ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! 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