Thermococcus gam

Thermococcus eur Thermococcus gor

Thermococcus sp.

Thermococcus gua

Thermococcus zill

Thermococcus nau r Thermococcus sp. P6

akarensis tonophilus

reducens Palaeococcus ferro urineus Palaeococcus paci zophilus

ossii

Thermococcales a 5-4 iotolerans tensis ThermococcusThermococcus sp. lito 4557 matolerans Thermococcus lito ythermalis ricrescens gonarius

Tree scale: 1 EXT12c ymasensis ificus li

Thermococcus sp. igii fundus Thermococcus sp. AM4 tili Thermococcus sp. philus Thermococcus cele ficus Thermococcus kod Thermococcus pep Thermococcus sibrchaeon 44_46 ThermococcusThermococcus thio Thermococcus onn pie

Thermococcus bar Thermococcus sib Thermococcus sp. 2319x1 Thermococcus rad ralis Thermococcus clef Thermococcus sp. ervidus ralis Thermococcus sp. Thermococcus cele Thermococcus bar Thermococcus pac 40_45 Thermococcus sicu Thermococcus pro Thermococcus bar PK iricus Thermococcus par iricus icicus EP1 ophilus Methanothermus f eus Pyrococcus yayan ophilus us vulcanius alvinellae us infernus Pyrococcus sp. NA us villosus Pyrococcus abyssi us villosus osii Methanotorris form ccus okinawensis Pyrococcus horiko Methanotorris ign Thermococcus chi 2 MethanocaldococcMethanocaldococc us bathoardescens Methanocaldococc us fervens AG86 tonophagusshii Methanocaldococc Pyrococcus kukulk Methanothermocous fervens Methanocaldococc us jannaschii Pyrococcus sp. STanii Methanocaldococc Methanocaldococc us sp. FS406-22 04 Methanocaldococc Pyrococcus furiosu Methanocaldococc s Pyrococcus furiosus DSM 3638

uitans Kin4-M Nanoarchaeum eq sidianus stetteri Candidatus Nanob Candidatus Nanobsidianus stetteri

Candidatus Korarc haeota archaeon

Candidatus Marsar iense TE7 Candidatus Marsar chaeota G1 archae ogun 4 chaeota G1 archae naticum DSM 1351 Candidatus Marsar on BE_D reducens Candidatus MarsarMarsar chaeota G2 archae Pyrobaculum arse Candidatus Marsar on OSP_D Pyrobaculumphilum ferri str.dicum IM2 P30 chaeota G2 archae on ECH_B_SAG-F0 Pyrobaculum aero chaeota G2 archae Pyrobaculumrophilum islan V24Sta chaeota G2 archae chaeota G2 archae on ECH_B_SAG-C1 8 Pyrobaculumifontis sp.JCM W 11548nax Methanopyrus kan on OSP_D Pyrobaculum neut . JCHS_4oniensis Methanopyrus sp. on ECH_B_2 6 Candidatus Korarc on ECH_B_3 Pyrobaculum calid ibuta Candidatus Korarc sp novskiaB80 Candidatus Korarc dleri Thermoproteus te Thermoproteus uz ophila 0 KOL6

Aciduliprofundum haeota archaeon ngensis AciduliprofundumAciduliprofundum

. ECH_B haeum cryptofilum VulcanisaetaVulcanisaeta mout distr Archaeoglobus ful sp. E Archaeoglobus ful haeum cryptofilum

Candidatus Geothe ArchaeoglobusArchaeoglobus ful ful

_217_2 Thermofilum sp. e

Thermofilum Archaeoglobus sul VulcanisaetaCaldivirga therm sp. MU8 21 FerroglobusArchaeoglobusArchaeoglobus placid ven pro

Geoglobus ahanga B24 Z13 ens sp. MAR08-339 Geoglobus acetivo ense neum ense Candidatus Bathya neum boonei Caldivirga maquili Aigarchaeota arch boonei OPF8

ex4484_135

Thermocladium sp ens Hrk 5 gidus ex4484_31 gidus gidus DSM 4304 archaeon ex4484 archaeon HR02 gidus DSM 4304 faticallidus

rmarchaeota archa

x4484_82 rchaeon ex4484_1 fundus rchaeum subterra us eficus rchaeum subterra rchaeota archaeon ri rchaeota archaeon Thermofilum sp. N Thermofilum pend Thermofilum uzon rans Thermofilum uzon aeon NZ13_MG1

rchaeota archaeon

Desulfurococcales chaeota archaeon Thermofilum pend

Candidatus Caldia Thaumarchaeota a eon ex4572_27 Candidatus Caldia

B24-2 Candidatus Bathya

Candidatus Bathya

Candidatus Pacear SI FIg. 9. Phylogenetic reconstruction of Archaeal RG dataset with TopR1-like and TopR2-like sequences removed. Branch labels show source of RG sequence