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Tree Scale: 1 Ext12c Ymasensis Ificus Li Thermococcus gor ThermococcusThermococcus gam eurThermococcus sp. P6 urineus zophilus r reducens ossii tonophilus 5-4 Thermococcus zill akarensis iotolerans tensis 4557 ricrescens Thermococcus gua Thermococcus nau ificus li Thermococcus sp. fundus PalaeococcusPalaeococcus ferro paci gonarius ervidus ythermalis matolerans EXT12c Thermococcus sp. Thermococcus Thermococcus pie Thermococcus onn Thermococcus cele Thermococcus Thermococcus thio Thermococcus igii Thermococcus bar Thermococcus sp. Thermococcus lito pep Thermococcus Thermococcus rad ymasensis kod Thermococcus Thermococcales a ThermococcusThermococcus clef sp. icicus ThermococcusThermococcus sp. lito tili Thermococcus cele AM4 Thermococcus pac Thermococcus sicu Thermococcus pro eus us vulcanius philusficus us infernus us villosus Methanothermus f Thermococcus sp. us villosus Thermococcusrchaeon sp. 44_46 ccus okinawensis ralis 2319x1 Thermococcus sib ralis us bathoardescens Thermococcus sib Methanotorris form us fervens AG86 Methanotorris ign us fervens Tree scale: 1 MethanocaldococcMethanocaldococc 40_45 PK Methanocaldococc us jannaschii Thermococcus sp. Methanocaldococc iricus Methanothermoco us sp. FS406-22 Thermococcus bar iricus Methanocaldococc Methanocaldococc Thermococcus bar Methanocaldococc EP1 Methanocaldococc Thermococcus par ophilus Methanocaldococc ophilus alvinellae Pyrococcus yayan osii uitans Kin4-M Pyrococcus sp. NA sidianus stetteri 2 sidianus stetteri Pyrococcus abyssi Nanoarchaeum eq Candidatus Nanob Pyrococcus horikoshii Candidatus Nanob haeota archaeon Thermococcus chitonophagus Candidatus Korarc anii on BE_D Pyrococcus kukulk 04 chaeota G1 archae on OSP_D chaeota G1 archae 8 Pyrococcus sp. ST s Candidatus Marsar on ECH_B_SAG-F0 Candidatus Marsarchaeota G2 archae Pyrococcus furiosu s DSM 3638 Candidatus Marsarchaeota G2 archaeon ECH_B_SAG-C16 Candidatus Marsarchaeota G2 archae Candidatus Marsar on OSP_D Pyrococcus furiosu chaeota G2 archae chaeota G2 archae on ECH_B_2 on ECH_B_3 Methanopyrus kan Methanopyrus sp. dleri Candidatus Korarc KOL6 CandidatusCandidatus Korarc Korarc haeota archaeon 4 iense TE7 haeum cryptofilum Aciduliprofundum haeum cryptofilum reducens AciduliprofundumAciduliprofundum Pyrobaculumnaticum ogun DSM 1351dicumP30 philum str. IM2 OPF8 Archaeoglobus ful Pyrobaculum ferri Archaeoglobus ful sp. MAR08-339 Pyrobaculum arse rophilum V24Sta nax ArchaeoglobusArchaeoglobusArchaeoglobus ful ful sul . JCHS_4 boonei Pyrobaculum islan oniensis boonei Pyrobaculum aero ifontis JCM 11548 ArchaeoglobusArchaeoglobus ven pro Pyrobaculum sp. W Ferroglobus placid ibutaB80 Candidatus Geothe 0 Candidatus Bathya novskia Geoglobus ahanga Aigarchaeota arch ophila Thermofilum sp. e Geoglobus acetivo Pyrobaculum neut gidus Thermofilum gidus faticallidus Thermoproteus te gidus DSM 4304 Thermoproteus sp ngensis gidus DSM 4304 ens Pyrobaculum calidThermoproteus uz Z13 . ECH_B ense ense 21 B24 _217_2 Vulcanisaeta distr 5 Hrk ens fundus Vulcanisaeta sp. E eficus rchaeota archaeon Vulcanisaeta mout us neum neum ex4484_135 aeon NZ13_MG1 Caldivirga sp. MU8 rmarchaeota archa Vulcanisaeta therm ri rans x4484_82 ex4484_31 Caldivirga maquili archaeon HR02 Thermocladium sp pend Thermofilum Thermofilum sp. N sp. Thermofilum Thermofilum uzon Thermofilum archaeon ex4484 uzon Thermofilum rchaeon ex4484_1 rchaeota archaeon B24-2 Thermofilum pend Thermofilum rchaeum subterra rchaeum subterra rchaeota archaeon eon ex4572_27 chaeota archaeon Desulfurococcales Thaumarchaeota a Candidatus Bathya Candidatus Caldia Candidatus Caldia Candidatus Bathya Candidatus Pacear SI FIg. 9. Phylogenetic reconstruction of Archaeal RG dataset with TopR1-like and TopR2-like sequences removed. Branch labels show species source of RG sequence.
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