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SEBAGAI ANTI HIPERGLIKEMIA PADA MENCIT (Mus Musculus)

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SEBAGAI ANTI HIPERGLIKEMIA PADA MENCIT (Mus Musculus) EFEKTIVITAS PEMBERIAN INFUSA DAUN SIRIH MERAH (Piper crocatum) SEBAGAI ANTI HIPERGLIKEMIA PADA MENCIT (Mus musculus) SKRIPSI Oleh: FUJI LESTARI NPM. 16820083 FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS WIJAYA KUSUMA SURABAYA SURABAYA 2020 ii iii EFEKTIVITAS PEMBERIAN INFUSA DAUN SIRIH MERAH (Piper crocatum) SEBAGAI ANTI HIPERGLIKEMIA PADA MENCIT (Mus musculus) Fuji Lestari ABSTRAK Penelitian ini bertujuan untuk mengetahui efektifitas pemberian infusa daun sirih merah (Piper crocatum) sebagai anti hiperglikemia pada mencit (Mus musculus). Hewan percobaan yang digunakan adalah mencit jantan sebanyak 24 ekor. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 6 ulangan. Keempat perlakuan tersebut adalah P0 (Kontrol), P1 (6 ekor mencit dengan pemberian infusa daun sirih merah konsentrasi 5%), P2 (6 ekor mencit dengan pemberian infusa daun sirih merah konsentrasi 10%), dan P3 (6 ekor mencit dengan pemberian infusa daun sirih merah konsentrasi 15%). Perlakuan tersebut dilakukan setiap satu hari sekali selama 21 hari. Sebelum dilakukan perlakuan, dilakukan penimbangan berat badan awal dan pemeriksaan kadar glukosa darah dilanjutkan pada hari ke-7, pada hari ke-14, dan pada hari ke-21. Data yang diperoleh dari hasil penimbangan berat badan ditemukan nilai rata-rata P0 (25,67±2,994a) g, P1 5% (24,17±1,329a) g, P2 10% (22,67±2,658a) g, dan P3 15% (24,83±2,994a). Hasil data dari pemeriksaan kadar gula darah ditemukan nilai rata- rata P0 (97,17±57,784a) mg/dL, P1 5% (98,83±52,943a) mg/dL, P2 10% (82,00±41,313a) mg/dL, dan P3 15% /(94,67± 41,793a) mg/dL. Data yang diperoleh selanjutnya dianalisis menggunakan uji ANOVA yang secara statistik menunjukkan tidak terdapat perbedaan nyata (P>0,05) dari berat badan dan kadar glukosa darah pada mencit (Mus musculus) yang diberi infusa daun sirih merah (Piper crocatum). Kata kunci: Infusa, Daun Sirih Merah, Anti Hiperglikemia iv EFFECTIVENESS OF GIVING RED BETEL LEAF INFUSION (Piper crocatum) AS ANTI HYPERGLYCEMIA IN MICE (Mus musculus) Fuji Lestari ABSTRACT The purpose of this research was to know the effectiveness of red betel leaf infusion (Piper crocatum) as anti hyperglycemia in mice (Mus musculus). Experimental animals used were 24 male mice. The design of this research was completely randomized design (CRD) with 4 treatments 6 repetitions. There were four treatmens P0 (Control), P1 (6 mice given 5% concentration of red betel leaf infusion), P2 (6 mice given 10% concentration of red betel leaf infusion), and P3 (6 mice given 15% concentration of red betel leaf infusion). The treatment is carried out once every day for 21 days. Before treatment, initial weight was measured and blood glucose level examination continued the 7th day, on 14th day, until the 21st day. Data obtained from the results of weighing found an average value of P0 (25.67 ± 2.994a) g, P1 5% (24.17 ± 1.329a) g, P2 10% (22.67 ± 2.658a) g, and P3 15% (24.83 ± 2.994a). The results of the data from the examination of blood glucose levels found an average value of P0 (97.17 ± 57.784a) mg/dL, P1 5% (98.83 ± 52.943a) mg/dL, P2 10% (82.00 ± 41.313a ) mg/dL, and P3 15% (94.67 ± 41.793a) mg/dL. The data obtained were then analyzed using the ANOVA test which statistically showed no significant difference (P> 0.05) from body weight and blood glucose levels in mice (Mus musculus) which were given red betel leaf infusion (Piper crocatum). Keywords: Infusion, Red Betel Leaf, Anti Hyperglycemia v vi KATA PENGANTAR Alhamdulillah, puji syukur penulis mengucapkan atas kehadirat Allah SWT yang telah melimpahkan rahmat dan karunia-Nya, sehingga penulis dapat menyelesaikan skripsi yang berjudul “Efektivitas Pemberian Infusa Daun Sirih Merah (Piper crocatum) Sebagai Anti Hiperglikemia Pada Mencit (Mus musculus)” Maksud dan tujuan penulisan ini adalah untuk memenuhi syarat menyelesaikan studi dan mendapatkan gelar Sarjana Kedokteran Hewan di Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya. Terwujudnya penulisan skripsi ini tidak terlepas dari bantuan, dan motivasi dari berbagai pihak. Oleh karena itu penulis mengucapkan terima kasih dengan tulus dan rasa hormat kepada : 1. Rektor Universitas Wijaya Kusuma Surabaya, Prof. H. Sri Harmadji., dr. Sp.THT-KL (K) yang telah memberikan ijin dan menerima penulis sebagai mahasiswa di Fakultas Universitas Wijaya Kusuma Surabaya. 2. Dekan Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya Prof. Dr. Rochiman Sasmita, MS., MM., drh. yang telah membantu kelancaran pendidikan penulis sebagai mahasiswa Kedokteran Hewan di Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya. 3. Dr. Miarsono Sigit,drh.,M.P selaku dosen Pembimbing Utama yang telah tekun membimbing, memberikan petunjuk, nasehat dan saran-saran, serta melakukan perbaikan atas skripsi ini sehingga selesai, dengan penuh perhatian dan kesabaran. vii 4. Desty Apritya,drh.,M.Vet., selaku dosen Pembimbing Pendamping yang telah membimbing, mengarahkan, memberi dorongan semangat dan mengoreksi skripsi ini dengan penuh kesabaran dan ketulusan. 5. Retina Yunani,drh.,M,Kes selaku dosen Penguji yang telah meluangkan waktu, pemikiran, kritik, saran, nasehat serta motivasi demi menyempurnakan skripsi. 6. Seluruh Dosen dan segenap staf Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya yang telah banyak membantu penulis dalam menyelesaikan studi. 7. Kepala Sekolah SMKN 1 Bontang bapak Drs. Kasman Purba,M.Pd yang sudah membantu dalam fasilitas penunjang penelitian 8. Kedua orang tua tercinta, Bapak Abriansyah dan Ibu Titi, Kakak dan Adik saya yang selalu memberikan dukungan, semangat, doa dan selalu mengorbankan segalanya sehingga terselesaikannya skripsi ini. 9. Semua teman-teman yang sudah mendoakan, memberi semangat dan membantu sehingga penulisan skripsi ini selesai Qusnul Chotimah, Amelia Yolanda, Nurwahyuni, Halimah, Rezka A, Wahyu Rafida, Nindy A dan kerabat lainnya yang tidak dapat saya sebutkan satu-persatu. 10. Teman-teman seperjuangan dan calon kolega FKH UWKS angkatan 2016 yang tidak bisa saya ucapkan satu persatu. Terimakasih sudah menjadi teman yang baik, semoga pertemanan ini tidak cukup sampai kita meraih gelar Dokter Hewan. 11. Keluarga Besar Minat Profesi Pet Animal Kedokteran Hewan Universitas viii Wijaya Kusuma Surabaya yang selalu memberikan pelajaran, rekan, keluarga, semangat, doa dan motivasi untuk mendorong penulis belajar menjadi pribadi yang lebih baik. 12. Kepada orang-orang yang telah berbaik hati untuk menyelipkan nama penulis didalam doanya. Kepada semua pihak yang sudah membantu penulis selama ini yang tidak dapat penulis di sebutkan satu persatu. Semoga Allah SWT melimpahkan rahmat serta karunia-Nya kepada semua pihak yang telah membantu penulis dengan tulus ikhlas dalam menyelesaikan pendidikan ini. Amin. Penulis menyadari bahwa skripsi ini masih jauh dari kesempurnaan, oleh sebab itu kritik dan saran sangat penulis harapkan demi kesempurnaan skripsi ini. Penulis berharap semoga skripsi ini bermanfaat bagi masyarakat dan semua pihak yang membaca. Amin. Surabaya, 18 Agustus 2020 Penulis ix DAFTAR ISI Halaman HALAMAN JUDUL .................................................................................... i HALAMAN PENGESAHAN ..................................................................... ii HALAMAN PERSETUJUAN PENGUJI ................................................. iii ABSTRAK ................................................................................................... iv ABSTRACT ................................................................................................. v HALAMAN PERNYATAAN ..................................................................... vi KATA PENGANTAR ........................................................................ vii DAFTAR ISI ................................................................................................ x DAFTAR TABEL ..................................................................................... xii DAFTAR GAMBAR ................................................................................. xiii DAFTAR LAMPIRAN ............................................................................. xiv I. PENDAHULUAN 1.1 Latar Belakang ................................................................................... 1 1.2 Rumusan Masalah .............................................................................. 4 1.3 Tujuan Penelitian ................................................................................. 4 1.4 Hipotesis ............................................................................................. 4 1.5 Manfaat Hasil Penelitian ...................................................................... 5 II. TINJAUAN PUSTAKA 2.1 Preparat Galenika ................................................................................ 6 2.1.1 Infusa ......................................................................................... 7 2.1.2 Solutio ........................................................................................ 7 2.2 Daun Sirih Merah (Piper crocatum) ..................................................... 8 2.2.1 Taksonomi Daun Sirih Merah (Piper crocatum) ......................... 9 2.2.2 Ciri Fisik Daun Sirih Merah (Piper crocatum) ......................... .. 9 2.2.3 Kandungan Kimia Daun Sirih Merah (Piper crocatum) ............ 10 2.2.3.1 Senyawa Flavonoid ...................................................... 10 2.2.3.2 Minyak Atsiri ............................................................... 11 2.2.3.3 Alkaloid ......................................................................
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