DOCSLIB.ORG

Listeria Innocua Nonpathogenic P60-Specific CD4 and CD8 T Cell

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Listeria Innocua Nonpathogenic P60-Specific CD4 and CD8 T Cell Enhancement of the Listeria monocytogenes p60-Specific CD4 and CD8 T Cell Memory by Nonpathogenic Listeria innocua This information is current as Gernot Geginat, Thomas Nichterlein, Marianne Kretschmar, of September 25, 2021. Simone Schenk, Herbert Hof, Mio Lalic-Mülthaler, Werner Goebel and Andreas Bubert J Immunol 1999; 162:4781-4789; ; http://www.jimmunol.org/content/162/8/4781 Downloaded from References This article cites 56 articles, 30 of which you can access for free at: http://www.jimmunol.org/content/162/8/4781.full#ref-list-1 http://www.jimmunol.org/ Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication by guest on September 25, 2021 *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 1999 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Enhancement of the Listeria monocytogenes p60-Specific CD4 and CD8 T Cell Memory by Nonpathogenic Listeria innocua1 Gernot Geginat,2* Thomas Nichterlein,* Marianne Kretschmar,* Simone Schenk,* Herbert Hof,* Mio Lalic-Mu¨lthaler,† Werner Goebel,† and Andreas Bubert3† The contact of T cells to cross-reactive antigenic determinants expressed by nonpathogenic environmental micro-organisms may contribute to the induction or maintenance of T cell memory. This hypothesis was evaluated in the model of murine Listeria monocytogenes infection. The influence of nonpathogenic L. innocua on the L. monocytogenes p60-specific T cell response was analyzed. We show that some CD4 T cell clones raised against purified p60 from L. monocytogenes cross-react with p60 purified from L. innocua. The L. monocytogenes p60-specific CD4 T cell clone 1A recognized the corresponding L. innocua p60 peptide QAAKPAPAPSTN, which differs only in the first amino acid residue. In vitro experiments revealed that after L. monocytogenes infection of APCs, MHC class I-restricted presentation of p60 occurs, while MHC class II-restricted p60 presentation is inhibited. Downloaded from L. innocua-infected cells presented p60 more weakly but equally well in the context of both MHC class I and MHC class II. In contrast to these in vitro experiments the infection of mice with L. monocytogenes induced a strong p60-specific CD4 and CD8 T cell response, while L. innocua infection failed to induce p60-specific T cells. L. innocua booster infection, however, expanded p60-specific memory T cells induced by previous L. monocytogenes infection. In conclusion, these findings suggest that infection with a frequently occurring environmental bacterium such as L. innocua, which is nonpathogenic and not adapted to intracellular replication, can contribute to the maintenance of memory T cells specific for a related intracellular pathogen. The Journal of http://www.jimmunol.org/ Immunology, 1999, 162: 4781–4789. isteria monocytogenes is a facultative intracellular bacte- which have been shown to play a role in the bacterial uptake by rium that can cause severe systemic infections in human various types of mammalian cells, do not seem to be present in Lgroups at risk, i.e., in immunosuppressed and elderly people, nonpathogenic Listeria species (10). as well as in neonates and pregnant women (1). It represents the The established model of the murine L. monocytogenes infection best studied member of the genus Listeria, which includes five is characterized by the development of a long-lasting T cell-de- additional species, L. ivanovii, L. innocua, L. seeligeri, L. welshi- pendent immunity (11). The murine L. monocytogenes infection by guest on September 25, 2021 meri, and L. grayi. In addition to L. monocytogenes the only other induces an initial induction and activation of Ag-specific effector T potentially pathogenic species of the genus is L. ivanovii (2). All cells followed by subsequent contraction by apoptosis and the es- other members are considered harmless environmental bacteria. L. tablishment of a T cell memory compartment (12, 13). It remains innocua shows the closest phylogenetic relationship to L. mono- uncertain whether the prolonged life span of memory T cells is an cytogenes (3) and is the most frequent listerial isolate from human intrinsic property of these cells or whether it reflects their inter- feces (4) and various food sources (5–8). Pathogenic and non- mittent stimulation by residual deposits of specific Ag, through pathogenic Listeria species differ in multiple pathogenicity-asso- cross-reactive contact with environmental Ags or through by- ciated gene loci. The gene products of the PrfA-dependent gene stander stimulation (14–16). The investigation of listerial target cluster, which are crucial for the release from the host cell phago- Ags recognized by CD4 and CD8 T cells identified several se- some, intracellular replication, intracellular movement, and the creted proteins of L. monocytogenes as potent T cell Ags. The cell-to-cell spread of pathogenic Listeria species (9), are lacking in listeriolysin O (LLO)4 and the p60 protein from L. monocytogenes L. innocua. In addition, genes coding for internalins, some of are targets for both CD4 and CD8 T cells (17–20). It has been shown that LLO-specific CD8 T cell clones and p60-specific CD4 or CD8 T cell clones mediate protective immunity against L. *Institut fu¨r Medizinische Mikrobiologie und Hygiene, Fakulta¨t fu¨r Klinische Medi- zin Mannheim der Universita¨t Heidelberg, Mannheim, Germany; and †Lehrstuhl fu¨r monocytogenes in vivo (20–22). In contrast to the PrfA-regulated Mikrobiologie, Theodor-Boveri-Institut fu¨r Biowissenschaften, Am Hubland, Wu¨rz- virulence factors of L. monocytogenes, the PrfA-independent p60 burg, Germany protein is essential for cell viability, and it acts basically as a Received for publication October 27, 1998. Accepted for publication January murein hydrolase required in a late step of cell division (23). It is 14, 1999. the major extracellular protein from pathogenic L. monocytogenes The costs of publication of this article were defrayed in part by the payment of page and contributes to the uptake of this pathogen into some mamma- charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. lian cell types such as fibroblasts and macrophages (23–26). Be- 1 This work was supported by the Forschungsfond der Fakulta¨t fu¨r Klinische Medizin cause of the essential function of p60 for bacterial cell division, it Mannheim der Universita¨t Heidelberg, Projekt 13/1998. M.L.-M. received a stipend is not surprising that a p60-related protein is produced by all mem- from the Graduiertenkolleg Infektiologie funded by the Deutsche Forschungsgemein- bers of the genus Listeria (25). The amino acid sequence compar- schaft. ison of the whole p60 protein family revealed that the amino- and 2 Address correspondence and reprint requests to Dr. G. Geginat, Institut fu¨r Mediz- inische Mikrobiologie und Hygiene, Fakulta¨t fu¨r Klinische Medizin Mannheim der Universita¨t Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany. E- 4 Abbreviations used in this paper: LLO, listeriolysin O; LM-p60, p60 produced by mail address: [email protected] Listeria monocytogenes; LI-p60, p60 produced by Listeria innocua; rmIFN, recom- 3 Current address: Microbiological Analytics, Merck KGaA, Darmstadt, Germany. binant murine IFN; ELISPOT, enzyme-linked immunospot. Copyright © 1999 by The American Association of Immunologists 0022-1767/99/$02.00 4782 NONPATHOGENIC LISTERIA AND T CELL MEMORY carboxyl-terminal portions of p60 are highly conserved in all Lis- Peptide libraries and synthetic peptides teria species, while the inner portions of p60 differ in a species- For mapping of the LI-p60 epitope recognized by the cross-reactive CD4 specific way (25). T cell clone 1A, soluble libraries of overlapping deca- and dodecapeptides The remarkable conservation of the p60 protein, which is a (offset 5 one amino acid) covering the previously identified antigenic re- strong target Ag for protective L. monocytogenes-specific CD4 and gion of the p60 molecule from L. innocua were synthesized. The synthesis CD8 T cells, provides an excellent model to study the influence of of a soluble peptide library representing the LM-p60 was described pre- viously (20). The individual peptide spots were excised and distributed into a related nonpathogenic species on the T cell-mediated immunity different 1.5-ml tubes for cleavage due to diketopiperazine formation at against a pathogenic bacterium. We show that infection with an neutral pH. The biological activity of individual peptides diluted 1/20 was environmental nonpathogenic bacterium such as L. innocua, which performed with a T cell activation assay as described below. Definitive by itself is unable to induce a primary p60-specific T cell response, amounts of peptides capable of inducing T cell proliferation were synthe- sized on a Zinsser (Frankfurt, Germany) Analytic SMPS 350 A peptide mediates the expansion of p60-specific memory T cells induced by synthesizer. previous L. monocytogenes infection. These findings suggest that contact with a frequently occurring harmless bacterium that is not Accessory cells adapted to an intracellular life can contribute to the maintenance of Spleen cells, B7-transfected P815 cells, or P388D1 cells were used as ac- memory T cells specific for an antigenically related intracellular cessory cells. Mitomycin C-inactivated spleen cells were prepared as de- pathogen. scribed previously (20) and were used as accessory cells in T cell prolif- eration assays and T cell activation assays.
Load more

Recommended publications
  • Inhibitory Activity of Lactobacillus Plantarum LMG P-26358 Against
    Mills et al. Microbial Cell Factories 2011, 10(Suppl 1):S7 http://www.microbialcellfactories.com/content/10/S1/S7 PROCEEDINGS Open Access Inhibitory activity of Lactobacillus plantarum LMG P-26358 against Listeria innocua when used as an adjunct starter in the manufacture of cheese Susan Mills1,3, L Mariela Serrano3, Carmel Griffin1,3, Paula M O’Connor1, Gwenda Schaad3, Chris Bruining3, Colin Hill2,4, R Paul Ross1,2*, Wilco C Meijer3 From 10th Symposium on Lactic Acid Bacterium Egmond aan Zee, the Netherlands. 28 August - 1 September 2011 Abstract Lactobacillus plantarum LMG P-26358 isolated from a soft French artisanal cheese produces a potent class IIa bacteriocin with 100% homology to plantaricin 423 and bacteriocidal activity against Listeria innocua and Listeria monocytogenes. The bacteriocin was found to be highly stable at temperatures as high as 100°C and pH ranges from 1-10. While this relatively narrow spectrum bacteriocin also exhibited antimicrobial activity against species of enterococci, it did not inhibit dairy starters including lactococci and lactobacilli when tested by well diffusion assay (WDA). In order to test the suitability of Lb. plantarum LMG P-26358 as an anti-listerial adjunct with nisin-producing lactococci, laboratory-scale cheeses were manufactured. Results indicated that combining Lb. plantarum LMG P- 26358 (at 108 colony forming units (cfu)/ml) with a nisin producer is an effective strategy to eliminate the biological indicator strain, L. innocua. Moreover, industrial-scale cheeses also demonstrated that Lb. plantarum LMG P-26358 was much more effective than the nisin producer alone for protection against the indicator. MALDI-TOF mass spectrometry confirmed the presence of plantaricin 423 and nisin in the appropriate cheeses over an 18 week ripening period.
    [Show full text]
  • Sanitation Assessment of Food Contact Surfaces and Lethality Of
    University of Arkansas, Fayetteville ScholarWorks@UARK Theses and Dissertations 5-2012 Sanitation Assessment of Food Contact Surfaces and Lethality of Moist Heat and a Disinfectant Against Listeria Strains Inoculated on Deli Slicer Components Sabelo Muzikayise Masuku University of Arkansas, Fayetteville Follow this and additional works at: http://scholarworks.uark.edu/etd Part of the Food Microbiology Commons Recommended Citation Masuku, Sabelo Muzikayise, "Sanitation Assessment of Food Contact Surfaces and Lethality of Moist Heat and a Disinfectant Against Listeria Strains Inoculated on Deli Slicer Components" (2012). Theses and Dissertations. 355. http://scholarworks.uark.edu/etd/355 This Thesis is brought to you for free and open access by ScholarWorks@UARK. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of ScholarWorks@UARK. For more information, please contact [email protected], [email protected]. SANITATION ASSESSMENT OF FOOD CONTACT SURFACES AND LETHALITY OF MOIST HEAT AND A DISINFECTANT AGAINST LISTERIA STRAINS INOCULATED ON DELI SLICER COMPONENTS SANITATION ASSESSMENT OF FOOD CONTACT SURFACES AND LETHALITY OF MOIST HEAT AND A DISINFECTANT AGAINST LISTERIA STRAINS INOCULATED ON DELI SLICER COMPONENTS A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Science By Sabelo Muzikayise Masuku Tshwane University of Technology Bachelor of Technology in Environmental Health, 2000 Curtin University Master of Public Health, 2005 May 2012 University of Arkansas ABSTRACT The overall objectives of this study were to: evaluate the efficacy of different cleaning cloth types and cloth-disinfectant combinations in reducing food contact surface contamination to acceptable levels; determine the optimum moist heat and moist heat + sanitizer treatments that can significantly reduce the number of Listeria strains on deli slicer components; and investigate if the moist heat treatment used in this study induced the viable-but-non-culturable (VBNC) state in Listeria cells.
    [Show full text]
  • Identification, Properties, and Application of Enterocins Produced by Enterococcal Isolates from Foods
    IDENTIFICATION, PROPERTIES, AND APPLICATION OF ENTEROCINS PRODUCED BY ENTEROCOCCAL ISOLATES FROM FOODS THESIS Presented in Partial Fulfillment of the Requirement for the Degree Master of Science in the Graduate School of The Ohio State University By Xueying Zhang, B.S. ***** The Ohio State University 2008 Master Committee: Approved by Professor Ahmed E. Yousef, Advisor Professor Hua Wang __________________________ Professor Luis Rodriguez-Saona Advisor Food Science and Nutrition ABSTRACT Bacteriocins produced by lactic acid bacteria have gained great attention because they have potentials for use as natural preservatives to improve food safety and stability. The objectives of the present study were to (1) screen foods and food products for lactic acid bacteria with antimicrobial activity against Gram-positive bacteria, (2) investigate virulence factors and antibiotic resistance among bacteriocin-producing enterooccal isolates, (3) characterize the antimicrobial agents and their structural gene, and (4) explore the feasibility of using these bacteriocins as food preservatives. In search for food-grade bacteriocin-producing bacteria that are active against spoilage and pathogenic microorganisms, various commercial food products were screened and fifty-one promising Gram-positive isolates were studied. Among them, fourteen food isolates with antimicrobial activity against food-borne pathogenic bacteria, Listeria monocytogenes and Bacillus cereus, were chosen for further study. Based on 16S ribosomal RNA gene sequence analysis, fourteen food isolates were identified as Enterococcus faecalis, and these enterococcal isolates were investigated for the presence of virulence factors and antibiotic resistance through genotypic and phenotypic screening. Results indicated that isolates encoded some combination of virulence factors. The esp gene, encoding extracellular surface protein, was not detected in any of the isolates.
    [Show full text]
  • Thesis Listeria Monocytogenes and Other
    THESIS LISTERIA MONOCYTOGENES AND OTHER LISTERIA SPECIES IN SMALL AND VERY SMALL READY-TO-EAT MEAT PROCESSING PLANTS Submitted by Shanna K. Williams Department of Animal Sciences In partial fulfillment of the requirements for the degree of Master of Science Colorado State University Fort Collins, Colorado Fall 2010 Master’s Committee: Department Chair: William Wailes Advisor: Kendra Nightingale John N. Sofos Doreene Hyatt ABSTRACT OF THESIS DETECTION AND MOLECULAR CHARACTERIZATION OF LISTERIA MONOCYTOGENES AND OTHER LISTERIA SPECIES IN THE PROCESSING PLANT ENVIRONMENT Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne disease associated with a high case fatality rate. To prevent product contamination with L. monocytogenes, it is crucial to understand Listeria contamination patterns in the food processing plant environment. The aim of this study was to monitor Listeria contamination patterns for two years in six small or very small ready-to-eat (RTE) meat processing plants using a routine combined cultural and molecular typing program. Each of the six plants enrolled in the study were visited on a bi-monthly basis for a two-year period where samples were collected, microbiologically analyzed for Listeria and isolates from positive samples were characterized by molecular subtyping. Year one of the project focused only on non-food contact environmental samples within each plant, and year two focused again on non-food contact environmental samples as well as food contact surfaces and finished RTE meat product samples from participating plants. Between year one and year two of sampling, we conducted an in-plant training session ii involving all employees at each plant.
    [Show full text]
  • Detección De Listeria Monocytogenes En Bovinos Y Ambientes De Predios
    UNIVERSIDAD DE LA REPÚBLICA FACULTAD DE VETERINARIA Programa de Posgrados DETECCIÓN DE LISTERIA MONOCYTOGENES EN BOVINOS Y AMBIENTE DE PREDIOS LECHEROS Estudio En Establecimientos Del Departamento De Paysandú Y En Un Caso Confirmado de Listeriosis Nerviosa CAROLINA MATTO ROMERO TESIS DE MAESTRÍA EN SALUD ANIMAL URUGUAY 2016 UNIVERSIDAD DE LA REPÚBLICA FACULTAD DE VETERINARIA Programa de Posgrados DETECCIÓN DE LISTERIA MONOCYTOGENES EN BOVINOS Y AMBIENTE DE PREDIOS LECHEROS Estudio En Establecimientos Del Departamento De Paysandú Y En Un Caso Confirmado de Listeriosis Nerviosa CAROLINA MATTO ROMERO R. Rivero DMV, MSc G. Varela MD, MSc, PhD R. Gianneechini, DMTV, MSc Director de Tesis Co-director Co-director 2016 INTEGRACIÓN DEL TRIBUNAL DE DEFENSA DE TESIS Pablo Zunino; DMV, MS, PhD Instituto de Investigaciones Biológicas “Clemente Estable” Ministerio de Educación y Cultura – Uruguay Franklin Riet-Correa; DMV, MS, PhD Instituto Nacional de Investigación Agropecuaria – Uruguay José Piaggio; DMTV, MS Facultad de Veterinaria Universidad de la República - Uruguay 2016 ACTA DE DEFENSA DE TESIS INFORME DEL TRIBUNAL AGRADECIMIENTOS En primer lugar, quiero expresar mi gratitud a mis Tutores: Rodolfo Rivero, Edgardo Gianneechini y Gustavo Varela por su constante apoyo, consejos, enseñanzas y tiempo brindado a lo largo del trabajo. A la Facultad de Veterinaria de la Universidad de la República, por el financiamiento del trabajo de investigación a través de la beca CSIC-PLANISA “Programa de Fortalecimiento de la Investigación de Calidad en Salud Animal”. Al Laboratorio de Bacteriología y Virología del Instituto de Higiene “Profesor Arnoldo Berta” de la Facultad de Medicina, Universidad de la República, por el apoyo al trabajo de laboratorio.
    [Show full text]
  • UK Standards for Microbiology Investigations
    UK Standards for Microbiology Investigations Identification of Listeria species, and other Non-Sporing Gram Positive Rods (except Corynebacterium) Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 3 | Issue no: 3.1 | Issue date: 29.10.14 | Page: 1 of 29 © Crown copyright 2014 Identification of Listeria species, and other Non-Sporing Gram Positive Rods (except Corynebacterium) Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website https://www.gov.uk/uk- standards-for-microbiology-investigations-smi-quality-and-consistency-in-clinical- laboratories. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see https://www.gov.uk/government/groups/standards-for-microbiology-investigations- steering-committee). The contributions of many individuals in clinical, specialist and reference laboratories who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: Standards Unit Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ E-mail: [email protected] Website: https://www.gov.uk/uk-standards-for-microbiology-investigations-smi-quality-
    [Show full text]
  • Predominance of Distinct Listeria Innocua and Listeria Monocytogenes in Recurrent Contamination Events at Dairy Processing Facilities
    microorganisms Article Predominance of Distinct Listeria Innocua and Listeria Monocytogenes in Recurrent Contamination Events at Dairy Processing Facilities Irene Kaszoni-Rückerl 1,2, Azra Mustedanagic 3, Sonja Muri-Klinger 1, Katharina Brugger 4, Karl-Heinz Wagner 2, Martin Wagner 1,3 and Beatrix Stessl 1,* 1 Unit of Food Microbiology, Institute of Food Safety, Food Technology and Veterinary Public Health, Department of Farm Animal and Public Health in Veterinary Medicine Department of Veterinary Public Health and Food Science, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria; [email protected] (I.K.-R.); [email protected] (S.M.-K.); [email protected] (M.W.) 2 Department of Nutritional Sciences, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090 Vienna, Austria; [email protected] 3 Austrian Competence Center for Feed and Food Quality, Safety and Innovation (FFOQSI), Technopark C, 3430 Tulln, Austria; azra.mustedanagic@ffoqsi.at 4 Unit of Veterinary Public Health and Epidemiology, Institute of Food Safety, Food Technology and Veterinary Public Health, Department of Farm Animal and Public Health in Veterinary Medicine Department of Veterinary Public Health and Food Science, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria; [email protected] * Correspondence: [email protected]; Tel.: +43-1-250-773-502 Received: 18 November 2019; Accepted: 6 February 2020; Published: 10 February 2020 Abstract: The genus Listeria now comprises up to now 21 recognized species and six subspecies, with L. monocytogenes and L. innocua as the most prevalent sensu stricto associated species. Reports focusing on the challenges in Listeria detection and confirmation are available, especially from food-associated environmental samples.
    [Show full text]
  • Prevalence of Listeria Species in Some Foods and Their Rapid Identification
    Yehia et al Tropical Journal of Pharmaceutical Research May 2016; 15 (5): 1047-1052 ISSN: 1596-5996 (print); 1596-9827 (electronic) © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved. Available online at http://www.tjpr.org http://dx.doi.org/10.4314/tjpr.v15i5.21 Original Research Article Prevalence of Listeria species in some foods and their rapid identification Hany M Yehia1,2*, Shimaa M Ibraheim3 and Wesam A Hassanein3 1Food Science and Nutrition Department, college of Food and Agriculture Sciences, King Saud University, Alriyadh, Saudi Arabia, 2Food Science and Nutrition, Faculty of Home Economics, Helwan University, Cairo, 3Department of Botany (Microbiology), Faculty of Science, Zagazig University, Zagazig, Egypt *For correspondence: Email: [email protected], [email protected]; Tel: 009660509610654 Received: 21 August 2015 Revised accepted: 13 April 2016 Abstract Purpose: To investigate the occurrence of Listeria spp., (particularly L. monocytogenes), in different foods and to compare diagnostic tools for their identification at species level. Methods: Samples of high protein foods such as raw meats and meat products and including beef products, chicken, fish and camel milk were analysed for the presence of Listeria spp. The isolates were characterised by morphological and cultural analyses, and confirmed isolates were identified by protein profiling and verified using API Listeria system. Protein profiling by SDS-PAGE was also used to identify Listeria spp. Results: Out of 40 meat samples, 14 (35 %) samples were contaminated with Listeria spp., with the highest incidence (50 %) occurring in raw beef products and raw chicken. Protein profiling by SDS- PAGE was used to identify Listeria spp.
    [Show full text]
  • Development of Listeriabase and Comparative Analysis of Listeria Monocytogenes Mui Fern Tan University of Malaya, Kuala Lumpur
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln CSE Journal Articles Computer Science and Engineering, Department of 2015 Development of ListeriaBase and comparative analysis of Listeria monocytogenes Mui Fern Tan University of Malaya, Kuala Lumpur Cheuk Chuen Siow University of Malaya, Kuala Lumpur Avirup Dutta University of Malaya, Kuala Lumpur Naresh VR Mutha University of Malaya, Kuala Lumpur Wei Yee Wee University of Malaya, Kuala Lumpur See next page for additional authors Follow this and additional works at: http://digitalcommons.unl.edu/csearticles Tan, Mui Fern; Siow, Cheuk Chuen; Dutta, Avirup; Mutha, Naresh VR; Wee, Wei Yee; Heydari, Hamed; Tan, Shi Yang; Ang, Mia Yang; Wong, Guat Jah; and Choo, Siew Woh, "Development of ListeriaBase and comparative analysis of Listeria monocytogenes" (2015). CSE Journal Articles. 127. http://digitalcommons.unl.edu/csearticles/127 This Article is brought to you for free and open access by the Computer Science and Engineering, Department of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in CSE Journal Articles by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Authors Mui Fern Tan, Cheuk Chuen Siow, Avirup Dutta, Naresh VR Mutha, Wei Yee Wee, Hamed Heydari, Shi Yang Tan, Mia Yang Ang, Guat Jah Wong, and Siew Woh Choo This article is available at DigitalCommons@University of Nebraska - Lincoln: http://digitalcommons.unl.edu/csearticles/127 Tan et al. BMC Genomics (2015) 16:755 DOI 10.1186/s12864-015-1959-5 DATABASE Open Access Development of ListeriaBase and comparative analysis of Listeria monocytogenes Mui Fern Tan1,2†, Cheuk Chuen Siow1†, Avirup Dutta1, Naresh VR Mutha1, Wei Yee Wee1,2, Hamed Heydari1,4, Shi Yang Tan1,2, Mia Yang Ang1,2, Guat Jah Wong1,2 and Siew Woh Choo1,2,3* Abstract Background: Listeria consists of both pathogenic and non-pathogenic species.
    [Show full text]
  • The Control and Management of Listeria Monocytogenes Contamination of Food
    The Control and Management of and Management The Control Listeria monocytogenes Contamination of Food Contamination of Food The Control and Management of Listeria monocytogenes Contamination of Food Food Safety Authority of Ireland Údarás Sábháilteachta Bia na hÉireann Abbey Court, Lower Abbey Street, Cúirt na Mainistreach, Sráid na Mainistreach Íocht., Dublin 1 Baile Átha Cliath 1 Telephone: +353 1 817 1300 Facsimile: +353 1 817 1301 E-mail: [email protected] Website: www.fsai.ie €15.00 Microbiology Listeria cover w9mm spine.indd 1 20/06/2005 09:54:06 The Control and Management of Listeria monocytogenes Contamination of Food Published by: Food Safety Authority of Ireland Abbey Court Lower Abbey Street Dublin 1 Telephone: +353 1 817 1300 Facsimile: +353 1 817 1301 E-mail: [email protected] Website: www.fsai.ie ©2005 ISBN 1-904465-29-3 Listeria cover w9mm spine.indd 2 20/06/2005 09:54:06 CONTENTS 1. Introduction 1 1.1 Background 1 1.2 Purpose of the Guidelines 1 1.3 Scope 1 1.4 What is Listeria monocytogenes? 2 1.5 What Foods are at Risk? 2 1.6 Health Risks and Clinical Features 3 1.7 Surveillance in Ireland 4 1.8 What are the Economic Consequences? 6 1.9 What are the Challenges? 6 1.10 Risk Communication 7 2. Factors Affecting Survival and Growth of L. monocytogenes 8 2.1 Introduction 8 2.2 Survival and Growth in Food 8 2.3 Survival and Growth in the Environment 9 2.4 Control of L. monocytogenes 10 3. General Pathogen Control in Food Processing 11 3.1 Introduction 11 3.2 Standard Operating Procedures 12 3.3 Purchasing and Delivery of Raw Materials 12 3.4 Facility Location, Design and Structure 13 3.5 Equipment Design 15 3.6 Zoning 16 3.7 Environmental and Storage Temperature 17 3.8 Water Supply 17 3.9 Maintenance 18 3.10 Sanitation 18 3.11 Personal Hygiene of Food Workers 19 3.12 Packaging 19 3.13 Storage and Distribution 19 3.14 Waste Management 19 3.15 Pest Control 20 3.16 Recall and Traceability 20 3.17 Food Safety Management (HACCP) 20 3.18 Recommendations 21 F OOD S AFETY A UTHORITY OF I RELAND 4.
    [Show full text]
  • Isolation and Characterization of Bacteriophages Against Listeria Monocytogenes and Their Applications As Biosensors for Foodborne Pathogen Detection
    Isolation and Characterization of Bacteriophages against Listeria monocytogenes and their Applications as Biosensors for Foodborne Pathogen Detection by Safaa Ahmed Othman Fallatah A Thesis Presented to The University of Guelph In partial fulfillment of requirements for the degree of Master of Science in Food Science Guelph, Ontario, Canada © Safaa Fallatah, February, 2018 ABSTRACT The Isolation and Characterization of Lytic Bacteriophages against Listeria Spp and their Applications for the Rapid Detection of L. monocytogenes in Food Contact Surface and Broth Safaa Fallatah Advisor: University of Guelph, 2018 Dr. Mansel Griffiths The aim of this study was to determine the potential application of bacteriophages for the detection of Listeria spp. on food contact surfaces (FCS). Eight phages were selected for further characterization to determine the most appropriate phage for use in a detection assay. They were characterized for host range, TEM, stability to air dying for 24 h at 25 °C, and restriction endonuclease pattern. L. monocytogenes strain C716 was very resistant to all phages, however; a mutated phage, AG13M, was able to infect L. monocytogenes strain C716. AG20 & AG23 phages were high specificity against their host (Listeria spp). AG20 phage was immobilized on ColorLok paper and used in the to detect L. monocytogenes C519 in broth and FCS. AG20 phage was able to detect as few as 50 CFU/mL of L. monocytogenes in TSB and 40 CFU/cm2 on FCS using a plaque assay to detect progeny phage within 24 h. ACKNOWLEDGMENTS In the name of Allah, the most gracious and the most merciful I would like to begin my thesis with the recognition that without his support and kindness I couldn’t done this thesis.
    [Show full text]
  • ABSTRACT DUTTA, VIKRANT. Characterization of Disinfectant And
    ABSTRACT DUTTA, VIKRANT. Characterization of Disinfectant and Phage Resistance Determinants of Listeria monocytogenes . (Under the direction of Dr. Sophia Kathariou). Environmental contamination of food plays a key role in transmission of Listeria monocytogenes . Adaptations such as ability to grow under refrigeration, biofilm formation, disinfectant resistance, and resistance to phage are crucial to the survival of L. monocytogenes in the environment. Here we have investigated some of the adaptations that may contribute towards environmental persistence of this pathogen. These include disinfectant (quaternary ammonium compounds, QAC) resistance, temperature-dependent resistance to phage among epidemic clone II strains, triphenylmethane dye reduction and resistance to heavy metals such as cadmium. Using in silico analysis, we have analyzed the prevalence of cadAC efflux systems associated with cadmium resistance among different Listeria genomes. Of the three distinct cadAC types known to date in L. monocytogenes , two ( cadA1C1 and cadA2C2 ) were harbored by plasmids and were encountered in diverse strains of L. monocytogenes and (in the case of cadA2C2 ) non-pathogenic Listeria spp. Large plasmids from two different epidemic- associated strains of L. monocytogenes harbored cadA2C2 together with a drug efflux system (bcrABC ), and both determinants were associated with a putative IS 1216 transposon. Plasmid-cured derivatives of these strains were susceptible both to cadmium and to BC. Genetic analyses revealed that cadA2C2 was sufficient for restoring resistance to cadmium to a plasmid-cured derivative of L. monocytogenes H7550. Furthermore, bcrABC was sufficient for restoring resistance to high levels of QAC to the plasmid-cured strain. The transcription of bcrABC was temperature-dependent, with higher transcript levels at 37°C than at 25°C or below.
    [Show full text]