DOCSLIB.ORG

Mona Mohamed Ali Ahmed

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Mona Mohamed Ali Ahmed Detection of Salmonella spp. Among School –Age Children at Wad Habouba Rural Hospital, Gezira State, Sudan (2018) Mona Mohamed Ali Ahmed (B.sc in Medical Microbiology Faculty of Medical Laboratory Sciences University of ElzaeimElazhary2005) Adissertation Submitted to the University of Gezira in Partial Fulfillment of the Requirements for the Award of the Degree of Master of Science In Medical Microbiology Faculty of Medical Laboratory Sciences October 2018 1 Detection of Salmonella spp. Among School –Age Children at Wad Habouba, Gezira State, Sudan (2018) Mona Mohammed Ali Ahmed Supervision Committee: Name Position Signature Prof. Adam Dawoud Abakar main supervisor ____________ Dr. Sanaa Elfatih Co- supervisor ____________ Date Examination:______________ 2 Detection of Salmonella spp.Among School –Age Children at WadHabouba, Gezira State, Sudan (2018) Mona Mohammed Ali Ahmed Examination Committee: Name Position Signature Prof. Adam Dawoud Abakar Chairman ____________ _________________________ External Examiner ____________ _________________________ Internal Examiner ____________ 3 Declaration We hereby, declare that this work is original research work; undertakenunder the supervision of Prof. Adam Dawoud Abakar and has not been presentedelsewhere for award of a degree of certificate. All sources have been citedand appropriately acknowledged. Name:____________________________ Date:_____________________________ Signature:_________________________ 4 Dedication Tothe greatest love in my life, thewoman whom I loved and give me the strength, well and support all the time Dearest mother… Tothose whom always on my side Brothers and Sisters… To my supporters through my way Friends… 5 Acknowledgements All praiseandthanks to Allah forblessing and for giving me the welling to complete this study,after that I would like to express my appreciation to my supervisor Prof. Adam DawoudAbakar Salim, for his kind help and support,after that I would like to express my appreciation to my supervisor Dr. Sanaa Alfatihalso thanks extended to all staff of the bacteriology laboratory of the medical laboratory of the Gezira University,Also acknowledge with gratitude the assistance,received from my colleges in the Wad Haboubarural hospital. Finally special thanks to everyone who was beside me to complete this work. 6 Detection of Salmonella spp.Among School –Age Children at Wad Habouba Rural, Gezira State, Sudan (2018) Mona Mohammed Ali Ahmed Abstract Enteric Fever is an important cause of morbidity and mortality amongchildren indevelopingcountriesincludingSudan.This cross sectional hospital based study aimed to estimate the prevalence rate and risk factor of enteric fever among student attend to wad habouba rural hospital,Blood samples and stools were collected from student aged between (6- 15 years) and suspected to be infected with enteric fever.The study was conducted during the period from August to November 2017 at Wad habouba hospital.Datawascollectedbystructured questionnaire.Specimens were examined by Widal test and stool culture,five ml of blood specimens were collected from student,Out of 100 blood sample only 37 were positive for widal test.stool sample out of 37 sample only 4 were positive in aculture,This study indicate low prevalence rate of enteric fever among the pupils in wad haboubaarea Further studies with large number of patients and more advanced techniques are required to validate the resultsof this study. It is better to perform culture for diagnosing salmonella species more than widal test. 7 الكشف عن انواع بكتريا السالمونيﻻ وسط اطفال المدارس في منطقة ود حبوبة-وﻻية الجزيرة – السودان منى محمد علي أحمد ملخص الدراسة تعد حمى التيفويدمن المسببات الهامة لﻻمراضية ومن اهم اﻻسباب تمعظم حاﻻت الوفاة ﻻطفال الدول النامية ويعد السودان احد الدول النامية، هدفت هذه الدراسة لتقدير معدل انتشار وعوامل خطورة حمي التيفويد من طﻻب مرحلة اﻻساس من منطقة ود حبوبة، تم جمع مائة عينة دم منالطﻻب الذين تتراوح اعمارهم بين سن الخامسة والخامسة عشر الذين يشتبه في انهم مصابين بحمي التايفويد في الفترة من اغسطس الي نوفمبر لعام 2017، من مائة عينة دم وجدت 37 عينة موجبة ﻻختبار الفيدال، ومن سبعة وثﻻثون عينة فسحة وجدت اربعة عينات موجبة في التزريع، وتشير هذه الدراسة الي انخفاض معدل انتشار اﻻصابة بحمي التيفويد بين الطﻻب في منطقة ود حبوبة وﻻية الجزيرة. اجراء مزيد من الدراسات مع عدد كبير من العينات والمزيد من التقنيات المتقدمة للتحقق من صحة نتائج هذه الدراسة.من اﻷفضل أداء التذريع لتشخيص أنواع السالمونيﻻ أكثر من اختبار الفيدال. 8 TABLEOF CONTENTS Serial Title Page Supervision committee i Examination Committee Ii Declaration iii Dedication iv Acknowledgements v English Abstract vi Arabic Abstract vi List of Content vii List of tables ix List of Figures x List of Abbreviations xi CHAPTER ONE: INTRODUCTION 1.1 Introduction 1 1.2 Justification 2 1.3 Topic identification 2 1.4 Objectives 2 1.4.1 General Objective 2 1.4.2. Specific Objectives 2 CHAPTER TWO: LITERATURE REVIEW 9 2.1 The organism 3 2.1.1 Classification 3 2.2 The disease 3 2.3 Symptoms 3- 4 2.4 Antigenic Structure 5-6 2.5. Epidemiology 6 2.6 Contamination And Transmission 7 2.7 Laboratory Diagnosis 7 2.7.1 Specimens 8 2.7.1.1 Blood 8 2.7.1.2 Stool 8 2.7.1.3 Serum 8 2.7.2 Microbiological Procedure 9 2.7.2.1 Blood Culture 9 2.7.2.2 Stool Culture 9 2.7.3 Colony Characteristics 9- 10 2.7.4 Serology 10-11 2.7.4.1 Widal Test 10-11 2.7.4.2 Haemagglutination 11 2.7.4.3 Counter Immuno Electrophoresis 111 2.9 Prevention 11-13 2.10 Anti-microbial Therapy 13 CHAPTER THREE MATERIALS AND METHODS 10 3.1 Study Area 14 3.1.1 Study Population 14 3.2 Study design 14 3.3 Inclusion Criteria 14 3.4 Exlusion Criteria 14 3.5 Sample Size 14 3.6 Design 15 3.7 Data Collection 15 3.8 Data Analysis 15 3.9 Ethical consideration 15 3.10 Materials 15-16 3.11 Laboratory methods 16-20 3.11.1 Preparation Of Media 16-17 3.11.2 Collection of specimens 17 3.11.2.1 Collection of blood 17 3.11.2.2 Collection of stool 17 3.11.3 Procedure 17 3.11.4 Culture 17 3.11.4.1 Colonial morphology 17-18 3.11.4.2 Gram smears 18 3.11.4.3 Biochemical tests 18-19 3.11.4.3.1 KIA 18-19 3.11.4.3.2 Indole 19 11 3.11.4.3.3 Urease 19 3.11.4.3.4 Citrate 19 CHAPTER FOUR 4.1 RESULTS 21-31 CHAPTER FIVE 5.1 Discussion 31 5.2 Conclusions 32 5.3 Recommendations 32 References 33 Appendix 37 12 List of Tables Table No. Title Page Table(4.1.1) Distribution of study population according to residence 22 Table (4.1.2) Distribution of study population according to washing hand 24 before having meal Table(4.1.3) Distribution of study population according to water source 26 Table (4.1.4) Presenting symptom frequencies Table(4.1.5) Resultfromdiagnostic test under study 29 Table(4.1.6) Some risk factor associated with infection 30 Table(4.1.7) Frequency and percentage of isolated organism 31 Table(4.1.8) Seroprevalence data together with personal and demographic 31 variables 13 14 Listof Figures FIGURES Title Page Figure (4.1.1) Distribution of study population according to gender 21 Figure(4.1.2) Distribution of study population according to water source 23 Figure(4.1.3) Washing hand with soap after toilet 25 Figure(4.1.4) Cleaning of dirt at home 27 15 List of Abbreviations Abbreviations Expansion ATP Adenosine triphosphate CIE Counter current immuno electrophoresis DCA Deoxycholate agar ELISA Enzyme linked immunosorbent assay HA Haemagglutination test HAg Flagellar Antigen LPS Lipopoly saccharides LPSHA Lipopolysaccharides heamagglutination MP Macrophage OAg Somatic Antigen RPHA Reverse passive haemagglutantion test SPP Species SS Salmonella Shigella VI Virulent Antigen XLD Xylose-Lysine-deoxycholate Agar 16 CHAPTER ONE Introduction 1.1 Background Salmonella spp are important food-borne pathogen associated with many feed stuffs. In order to minimize & control these sources of human infection efforts should be made at all levels of food product supply-chain. Typhoid fever, also known as enteric fever is a bacterial infect caused by a gram -ve bacteria primarily by salmonella enterica, subspecies enteric aserovar typhoid & to lesser extent related serovar's paratyphoid A, B, C.(Chart etal.2007) Both causes symptoms mild to severe and usually begin six to thirty days after exposure. Often there is gradual onset of fever over several days, weakness, abdominal pain, constipation & headache. Some people develop rash with rose coloured spots. In severe cases there may be confusion. Some people may carry the bacterium without being affected, however they are still spread the disease to others (Prajpati etal.2008). Poor hygiene habits & public sanitation conditions are main routes the disease spreading so an epidemiological investigation is need to be performed to determine the prevalence rate & subsequently to evaluate appropriate intervention polices, determine control programs. In order to estimate the prevalence of salmonella & to evaluate. The effectiveness of the intervention accurately as possible, it is necessary to investigate bacteriology & serology method more thoroughly (Prajpati etal 2008). Serology is one approach for determining salmonella status at the individual or population level & it is performed by widal test, which demonstrates antibodies against salmonella antigen O – somatic & H–Flagellar & It is used extensively in serodiagnosis of typhoid fever (Olopoenia and King 2000). On other hand, stool culture: is more useful in the diagnosis of enteric fever. 17 1-2 Research Justification 1-Typhoid fever remain an important public health problem in Sudan mainly in rural areas, but its screaning is not done in the study area. 2-Scare information available regarding its prevalence in study area. 3-School age children are frequently presented to health care center and clinics with symptoms resembling typhoid fever.
Load more

Recommended publications
  • Produktkatalog 2015/2016
    Produktkatalog 2015/2016 BD Life Sciences Diagnostic Systems Deutschland . Österreich . Schweiz BD stellt sich vor BD Lifeweltweit Sciences – BD – LifeBD MedicalSciences – BD Medical BD – weltweit BD Life Sciences – Biosciences BD Life Sciences – Diagnostic Systems BD, eines der führenden Medizintech- Biosciences bedient den gesamten Diagnostic Systems ist Ihr Experte und nologie-Unternehmen, hat es sich zur Bereich der biologischen und medizi- erfahrener Partner für die klinische Aufgabe gemacht, in Zusammenar- nischen Forschung und bietet die und industrielle mikrobiologische Diag- beit mit seinen Kunden und Partnern größte Auswahl an innovativen Gerä- nostik. den gegenwärtigen und zukünftigen ten und Reagenzien für die zelluläre Umfassende Konzepte und daran ge- Herausforderungen bei der Gesund- Analyse an. knüpfte Diagnostika, Softwarelösun- heitsversorgung von Menschen auf Ein breites Spektrum an Analyzern gen und Dienstleistungen führen zu der ganzen Welt zu begegnen. und Sortern für die Durchflusszyto- gesteigerter Effizienz im Labor und Mit innovativen Lösungen optimiert metrie, verbunden mit kompetenter zu schnelleren verwertbaren Ergeb- BD die Arzneimittelverabreichung, Beratung und Service, ermöglichen nissen. verbessert die Diagnose von Infek- die passgenaue Lösung für Ihr Labor. Unser klinischer Fokus liegt in der Prä- tionskrankheiten und Krebs, unter- Unser Produktangebot und unsere vention und Diagnose von Infektions- stützt die Behandlung von Diabetes Kompetenz machen uns zum interes- krankheiten sowie des Zervixkarzinoms. und bringt die Zellforschung voran. santen Partner für Forschungs- und Für den klinischen Bereich bietet Diag- Rund 30.000 BD-Mitarbeiterinnen Routinelabors, für die Industrie sowie nostic Sytems integrierte Lösungen und Mitarbeiter in 50 Ländern setzen für die wachsende Zahl von bio- und auf den Gebieten Tuberkulose, Sep- sich dafür ein, unser Unternehmens- gentechnologischen Einrichtungen.
    [Show full text]
  • OXOID MANUAL PRELIMS 16/6/06 12:18 Pm Page 1
    OXOID MANUAL PRELIMS 16/6/06 12:18 pm Page 1 The OXOID MANUAL 9th Edition 2006 Compiled by E. Y. Bridson (substantially revised) (former Technical Director of Oxoid) Price: £50 OXOID MANUAL PRELIMS 16/6/06 12:18 pm Page 2 The OXOID MANUAL 9th Edition 2006 Compiled by E. Y. Bridson (substantially revised) (former Technical Director of Oxoid) 9th Edition 2006 Published by OXOID Limited, Wade Road, Basingstoke, Hampshire RG24 8PW, England Telephone National: 01256 841144 International: +44 1256 841144 Email: [email protected] Facsimile National: 01256 463388 International: +44 1256 463388 Website http://www.oxoid.com OXOID SUBSIDIARIES AROUND THE WORLD AUSTRALIA DENMARK NEW ZEALAND Oxoid Australia Pty Ltd Oxoid A/S Oxoid NZ Ltd 20 Dalgleish Street Lunikvej 28 3 Atlas Place Thebarton, Adelaide DK-2670 Greve, Denmark Mairangi Bay South Australia 5031, Australia Tel: 45 44 97 97 35 Auckland 1333, New Zealand Tel: 618 8238 9000 or Fax: 45 44 97 97 45 Tel: 00 64 9 478 0522 Tel: 1 800 331163 Toll Free Email: [email protected] NORWAY Fax: 618 8238 9060 or FRANCE Oxoid AS Fax: 1 800 007054 Toll Free Oxoid s.a. Nils Hansen vei 2, 3 etg Email: [email protected] 6 Route de Paisy BP13 0667 Oslo BELGIUM 69571 Dardilly Cedex, France PB 6490 Etterstad, 0606 Oxoid N.V./S.A. Tel: 33 4 72 52 33 70 Oslo, Norway Industriepark, 4E Fax: 33 4 78 66 03 76 Tel: 47 23 03 9690 B-9031 Drongen, Belgium Email: [email protected] Fax: 47 23 09 96 99 Tel: 32 9 2811220 Email: [email protected] GERMANY Fax: 32 9 2811223 Oxoid GmbH SPAIN Email: [email protected] Postfach 10 07 53 Oxoid S.A.
    [Show full text]
  • Laboratory Diagnosis of Shigella and Salmonella Infections *
    Bull. Org. mond. Sante) 1959, 21, Bull. Wid Hlth Org. 247-277 Laboratory Diagnosis of Shigella and Salmonella Infections * E. HORMAECHE, M.D.' & C. A. PELUFFO, M.D.2 In recent years a great deal of work has been We are aware that experienced bacteriologists devoted to the study of enteric diseases and, in will prefer some of the methods they are using to consequence, many methods have been developed the ones we recommend. This study is not in- for the isolation of their most common agents, tended for them, however, but rather as a guide to Shigella, Salmonella and some pathogenic Esche- people who are just starting work on enteric diseases richia, while at the same time new tests have been diagnosis. For more detailed information, we devised to differentiate the " groups " of the Entero- recommend the books of Kauffmann (1954) and bacteriaceae family, so that the beginner, especially, Edwards & Ewing (1955), of which we have made will be faced with the difficulty of selecting from ample use. among them those to be used. Investigation of enteric pathogens will sometimes Each author has his preferences according to his be the ultimate purpose, as for the public health own experience, and it is regrettable that up to now officer, who is primarily interested in finding them no serious effort has been made to standardize for the potential danger they represent to the com- techniques, which would save a lot of time and dis- munity. In clinical medicine, however, the role couraging failures for the bacteriologist unex- played by the bacteriologist does not end there.
    [Show full text]
  • Medios En Placas Preparadas
    Medios de cultivo Hemoderivados, Medios en placas preparadas Sheep Blood in Alsevers Solution Iso-Sensitest Agar (Placa profunda) 25 mL SR0053B 10 x 90 mm PO0779A Iso-Sensitest Agar with Chocolated Horse Blood Sheep Blood Defibrinated 10 x 90 mm PB0147A 25 mL SR0051B Iso-Sensitest Agar with Horse Blood 10 x 90 mm PB0146A Medios en placas preparadas Iso-Sensitest Agar with Horse Blood and 20mg/L NAD 10 x 90 mm PB0378A Agares sangre Iso-Sensitest Agar con sangre de caballo lisada Blood Agar Base No. 2 with Horse Blood 10 x 90 mm PB0145A 10 x 90 mm PB0114A Iso-Sensitest Agar with Sheep Blood Blood Agar Base No. 2 with Sheep Blood 10 x 90 mm PB5003A 10 x 90 mm PB0115A Mueller Hinton Agar Columbia Agar with Horse Blood 10 x 90 mm PO5007A 10 x 90 mm PB0122A Mueller-Hinton Agar with 2% Salt Columbia Agar with Sheep Blood 10 x 90 mm PO0799A^ 10 x 90 mm PB5008A 10 x 90 mm PO5139A+ Columbia Agar with Sheep Blood PLUS Mueller-Hinton Agar 10 x 90 mm PB0123A*^ 10 x 90 mm PO0152A^ 10 x 90 mm PB5039A+ 10x120mm PO1191S Columbia Agar with Chocolated Horse Blood Mueller-Hinton Agar with 5% Sheep Blood 10 x 90 mm PB0124A 10 x 90 mm PB0431A^ 10 x 90 mm PB5007A+ Agares generales para anaerobios Mueller Hinton Agar with Horse Blood Thermo ScientificTM A.R.I.A.TM with Horse Blood 10 x 90 mm PB1229A^ 10 x 90 mm PB1243A 10 x 90 mm PB5303A+ A.R.I.A.
    [Show full text]
  • Shigella Dysenteriae Type 1
    Guidelines for the control of shigellosis, including epidemics due to Shigella dysenteriae type 1 World Health Organization TABLE OF CONTENTS i WHO Library Cataloguing-in-Publication Data World Health Organization. Guidelines for the control of shigellosis, including epidemics due to Shigella dysenteriae 1. 1.Dysentery, Bacillary - prevention and control 2.Shigella dysenteriae - pathogenicity 3.Disease outbreaks - prevention and control 4.Guidelines I.Title. ISBN 92 4 159233 0 NLM Classification: WC 282) © World Health Organization 2005 All rights reserved. Publications of the World Health Organization can be obtained from WHO Press, World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland (tel: +41 22 791 2476; fax: +41 22 791 4857; email: [email protected]). Requests for permission to reproduce or translate WHO publications – whether for sale or for noncommercial distribution – should be addressed to WHO Press, at the above address (fax: +41 22 791 4806; email: [email protected]). The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the World Health Organization concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. Dotted lines on maps represent approximate border lines for which there may not yet be full agreement. The mention of specific companies or of certain manufacturers’ products does not imply that they are endorsed or recommended by the World Health Organization in preference to others of a similar nature that are not mentioned. Errors and omissions excepted, the names of proprietary products are distinguished by initial capital letters.
    [Show full text]
  • Labplux Medical Brochure
    LABPLUX GLOBAL RESOURCES LTD Supplier of international quality scientific laboratory and medical equipment. LABPLUX GLOBAL RESOURCES LTD 2 13 CORPORATE PROFILE 12 3 MICRBIOLOGICAL MEDIA 1. A1 Broth 27. Mannitol salt agar BIOLOGICAL STAINS 2. Agar plate 28. Middlebrook 7H10 agar 3. Ashdown's medium 29. Middlebrook 7H11 agar 1. Acridine orange 4. Bile esculin agar 30. Middlebrook 7H9 broth 2. Bismarck brown 5. Bismuth sulphite agar 31. MRS agar 3. Carmine 6. Bordet-Gengou agar 32. Mueller-Hinton agar 4. Coomassie blue 7. Brain heart infusion broth 33. Nutrient agar 5. Crystal violet 8. Brucella agar 34. Plate count agar 6. DAPI 9. Casein nutrient agar 35. PNP agar 7. Eosin 10. Chocolate agar 36. Potato dextrose agar 8. Ethidium bromide 11. Chu 13 37. Rappaport Vassilladis Soya Peptone Broth 9. Acid Fuchsine 12. Cysteine lactose electrolyte deficient agar 38. Sabouraud agar 10. Haematoxylin 13. Cysteine trypc agar 39. Selenite broth 11. Hoechst stains 14. DCA Agar 40. Simmons' Citrate agar 12. Iodine 15. Dermatophyte test medium 41. Sorbitol-MacConkey agar 13. Malachite green 16. Eaton's agar 42. Super opmal broth 14. Methyl green 17. Endo agar 43. Thayer Marn agar 15. Methylene blue 18. Hektoen enteric agar 44. Thioglycollate broth 16. Neutral red 19. Hoyle's agar 45. Thiosulphate Citrate-Bile salts-Sucrose agar (TCBS) 17. Nile blue 20. Lauryl tryptose broth 46. Trypc Soy broth 18. Nile red 21. Lowestein-jensen medium 47. Trycase soy agar 19. Osmium tetraoxide 22. Lysine iron agar slant 48. TSI slant 20. Rhodamine 23. Lysogeny broth 49. VRBD agar 21.
    [Show full text]
  • WHO | World Health Organization
    WHO/CDR/95.4 Guidelines for the control of epidemics due to Shigella dysenteriae type 1 World Health Organization Emerging and other Communicable Diseases, Surveillance and Control This document has been downloaded from the WHO/EMC Web site. The original cover pages and lists of participants are not included. See http://www.who.int/emc for more information. © World Health Organization This document is not a formal publication of the World Health Organization (WHO), and all rights are reserved by the Organization. The document may, however, be freely reviewed, abstracted, reproduced and translated, in part or in whole, but not for sale nor for use in conjunction with commercial purposes. The views expressed in documents by named authors are solely the responsibility of those authors. The mention of specific companies or specific manufacturers' products does no imply that they are endorsed or recommended by the World Health Organization in preference to others of a similar nature that are not mentioned. shig4g 28.03.95 TABLE OF CONTENTS 1. Introduction...............................................................................................................................1 2. About Shigella dysenteriae type 1 (Sd1) ................................................................................1 3. Other causes of dysentery.......................................................................................................2 4. Prevention of infection with Shigella dysenteriae type 1 ....................................................2
    [Show full text]
  • MC Oxoid P35 37 Sept 06 V3 12/15/06 9:33 AM Page 35
    MC Oxoid p35_37 Sept 06 v3 12/15/06 9:33 AM Page 35 aseptic technology AA strategystrategy forfor microbemicrobe monitoringmonitoring suits. If this process is not performed correctly, contaminants can enter the cleanroom on gowns, gloves, over- shoes, etc. The garments worn in the cleanroom must fit well, so that skin Figure 1: Typical settle particles are retained within the suit, and contact plates and be made of a material that does not shed fibres or particles. Aseptic tech- niques should be taught in order that Key to any cleanroom or aseptic operation is a planned personnel do not contaminate gloves after, for example, touching their faces. programme of monitoring. Colin Booth, vice-president Water is a potential vector of conta- mination, enabling organisms to spread science and technology at Oxoid, outlines the basis for a in ways they cannot on solid surfaces. Regulations state that there should be microbial monitoring strategy no water outlets in cleanroom areas; however, water is used in cleaning and here it is not possible to good cleanroom design; effective air is probably the most commonly used terminally sterilise, to be handling; cleaning and disinfection; raw material in manufacture. Water- confident of producing a staff training for adherence to aseptic based fluids must be sterilised before sterile product manufac- techniques; properly fitted cleanroom they are taken into the filling rooms. W turers of parenteral drugs gowns; sterilisation of materials As water is also used in cleanroom sup- must ensure that a robust environ- brought into the aseptic environment; port areas, pharmaceutical grade water mental monitoring programme is in adequate monitoring of controlled con- should be used wherever possible, and place within aseptic processing areas ditions (such as air flow and air pres- it should be microbiologically con- (cleanrooms).
    [Show full text]
  • 2016 -2017 Microbiology Products Catalog
    2016 -2017 Microbiology Products Catalog Clinical • Food & Beverage • Pharmaceutical • Environmental • Veterinary HOW TO ORDER Contact details Technical Support helpline Oxoid Limited Our Web site is intended to make it easier for you to ask questions Wade Road or raise issues with us. Simply go to www.thermoscientific.com/ Basingstoke microbiology, and choose the most appropriate option in “Contact Us”. Hampshire RG24 8PW Technical enquiries can also be made by telephone, by fax or online: UK Tel: +44 (0) 1256 694238 Fax: +44 (0) 1256 329728 Tel: +44 (0) 1256 841144 Email: [email protected] Fax: +44 (0) 1256 334994 Email: [email protected] Web: www.thermoscientific.com/microbiology UK Office Hours: Mon-Fri 08.00 - 17.00 Sat & Sun closed Contents Antimicrobial Susceptibility Testing 8 Diagnostics 61 AST MIC Strips 8 Biochemical Identification 61 AST Accessories 9 Immunological Tests 63 AST Media 9 Blood and Serum Tests 65 Antimicrobial Susceptibility Testing Discs 10 Toxin Detection Kits 66 Antifungal Susceptibility Testing Discs 12 Agglutination Tests 66 AST Microbroth Dilution Plates 13 Enzyme Immunoassays 69 AST Microbroth Dilution Instrumentation and Accessories 14 ELISA Accessories 71 AST Microbroth Dilution Media 14 Food Allergen ELISA Assays 71 Immunofluorescence Assays 71 Incubators 15 Lateral Flow Assays 72 Atmosphere Generation 16 Rapid Food Tests 73 Reagents and Stains 73 AGS 16 Laboratory Supplies 74 Blood Culture 17 Molecular Products 75 Manual Blood Culture Systems 17 Sample Prep for
    [Show full text]
  • Identification of Shigella Species
    UK Standards for Microbiology Investigations 2013 Identification of Shigella species DECEMBER 13 - NOVEMBER 15 BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 20 | Issue no: di+ | Issue date: dd.mm.yy <tab+enter> | Page: 1 of 20 © Crown copyright 2013 Identification of Shigella species Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website http://www.hpa.org.uk/SMI/Partnerships. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see http://www.hpa.org.uk/SMI/WorkingGroups). The contributions of many individuals in clinical, specialist and reference laboratories2013 who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: DECEMBER 13 Standards Unit - Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ NOVEMBER E-mail: [email protected] 15 Website: http://www.hpa.org.uk/SMI UK Standards for Microbiology Investigations are produced in association with: BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Bacteriology – Identification | ID 20 | Issue no: di+ | Issue date: dd.mm.yy <tab+enter> | Page: 2 of 20 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Shigella species Contents ACKNOWLEDGMENTS .......................................................................................................... 2 AMENDMENT TABLE ............................................................................................................
    [Show full text]
  • Dspace@Balıkesir
    T.C. BALIKESİR ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ BİYOLOJİ ANABİLİM DALI RUSCUS ACULEATUS L. VE PUNICA GRANATUM L. BİTKİLERİNİN EKSTRELERİNİN VE BOYANMIŞ KUMAŞ ÖRNEKLERİNİN ANTİMİKROBİYAL ÖZELLİKLERİNİN BELİRLENMESİ YÜKSEK LİSANS TEZİ PINAR ÇELİKBOYUN BALIKESİR, MAYIS - 2015 T.C. BALIKESİR ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ BİYOLOJİ ANABİLİM DALI RUSCUS ACULEATUS L. VE PUNICA GRANATUM L. BİTKİLERİNİN EKSTRELERİNİN VE BOYANMIŞ KUMAŞ ÖRNEKLERİNİN ANTİMİKROBİYAL ÖZELLİKLERİNİN BELİRLENMESİ YÜKSEK LISANS TEZI PINAR ÇELİKBOYUN BALIKESİR, MAYIS - 2015 KABUL VE ONAY SAYFASI Pınar ÇELİKBOYUN tarafından hazırlanan “RUSCUS ACULEATUS L. VE PUNİCA GRANATUM L. BİTKİLERİNİN EKSTRELERİNİN VE BOYANMIŞ KUMAŞ ÖRNEKLERİNİN ANTİMİKROBİYAL ÖZELLİKLERİNİN BELİRLENMESİ” adlı tez çalışmasının savunma sınavı 21.05.2015 tarihinde yapılmış olup aşağıda verilen jüri tarafından oy birliği / oy çokluğu ile Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı Yüksek Lisans Tezi olarak kabul edilmiştir. Jüri Üyeleri İmza Danışman Doç. Dr. Tülin AŞKUN Üye Prof. Dr. Gülendam TÜMEN Üye Prof. Dr. Rengin ELTEM Jüri üyeleri tarafından kabul edilmiş olan bu tez Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Yönetim Kurulunca onanmıştır. Fen Bilimleri Enstitüsü Müdürü Doç. Dr. Necati ÖZDEMİR ............................................. Bu tez çalışması T.C. Bilim Sanayi ve Teknoloji Bakanlığı San-Tez Arge Destek Programı 0799.STZ.2011-1 nolu proje ve BAP tarafından 2013/54 nolu proje ile desteklenmiştir. ÖZET RUSCUS ACULEATUS L. VE PUNİCA GRANATUM L. BİTKİLERİNİN
    [Show full text]
  • Shigellosis and Socio-Demography of Hospitalized Patients in Kano, North-West, Nigeria
    International Journal of Pharmaceutical Science Invention ISSN (Online): 2319 – 6718, ISSN (Print): 2319 – 670X www.ijpsi.org Volume 6 Issue 3 ‖March 2017 ‖ PP. 31-37 Shigellosis and Socio-Demography of hospitalized Patients in Kano, North-West, Nigeria. *Abdullahi, Mas’ud, Department of Medical Laboratory Science, School of Health Technology, Kano, Kano state, Nigeria Abstract: Aim: The aim of the study was to determine the prevalent of Shigellosis in relation to socio-demographic characteristics of hospitalized patients in Kano metropolis. Study design: The study is a descriptive cross-sectional study. Place and duration of study: One milliliter of venous blood was collected from each patient with some or all clinical features of Shigellosis that sign a consent form and transfer into EDTA bottles. If daily is unavoidable blood samples were stored at 4 0C. Samples were analyzed at the both laboratories of the authors. This work was carried out between May, 2012 and March, 2014. Methodology: The blood specimens were cultured in thioglycollate broth and sub-cultured onto deoxycholate citrate agar (DCA), Salmonella-Shigella agar (SSA) and brilliant Green agar (BGA) followed by confirmation of presumptive colonies using different biochemical tests and analytical profile index 20E. Serologic identification of Shigella was performed by slide agglutination test using polyvalent O and H Shigella antisera. Results: Although, the relationship between different age groups was not significantly associated (P < 0.05), patients under age bracket of 21-30 years were found to be more susceptible to Shigella infections with 13 representing 2.6% followed in that order by 11-20 years (6), , ≤10 -years (4) 31-40 years (3) and >40 years (2) age groups, representing 1.2%, 0.8%, 0.6% and 0.4% respectively.
    [Show full text]