RESEARCH ARTICLE First Assessment of the Sex Ratio for an East Pacific Green Sea Turtle Foraging Aggregation: Validation and Application of a Testosterone ELISA Camryn D. Allen1*, Michelle N. Robbins1, Tomoharu Eguchi1, David W. Owens2, Anne B. Meylan3, Peter A. Meylan4, Nicholas M. Kellar1, Jeffrey A. Schwenter2, Hendrik H. Nollens5, Robin A. LeRoux1, Peter H. Dutton1, Jeffrey A. Seminoff1 1 Marine Mammal and Turtle Division, Southwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, La Jolla, California, United States of America, 2 Grice Marine Lab, University of Charleston South Carolina, Charleston, South Carolina, United States of America, 3 Florida Fish and Wildlife Conservation Commission, Fish and Wildlife Research Institute, St. Petersburg, Florida, United States of America, 4 Natural Sciences, Eckerd College, St. Petersburg, Florida, United States of America, 5 Veterinary Services, SeaWorld San Diego, San Diego, California, United States of America OPEN ACCESS *
[email protected] Citation: Allen CD, Robbins MN, Eguchi T, Owens DW, Meylan AB, Meylan PA, et al. (2015) First Assessment of the Sex Ratio for an East Pacific Green Sea Turtle Foraging Aggregation: Validation Abstract and Application of a Testosterone ELISA. PLoS ONE 10(10): e0138861. doi:10.1371/journal.pone.0138861 Determining sex ratios of endangered populations is important for wildlife management, particularly species subject to sex-specific threats or that exhibit temperature-dependent Editor: Daniel E Crocker, Sonoma State University, UNITED STATES sex determination. Sea turtle sex is determined by incubation temperature and individuals lack external sex-based traits until sexual maturity. Previous research utilized serum/ Received: May 13, 2015 plasma testosterone radioimmunoassays (RIA) to determine sex in immature/juvenile sea Accepted: September 4, 2015 turtles.