Polyphasic Taxonomy Leading to the Proposal of Moraxella Canis Sp. Nov
INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, July 1993, p. 438-449 Vol. 43, No. 3 0020-7713/93/030438-12$02.00/0 Copyright 0 1993, International Union of Microbiological Societies Polyphasic Taxonomy Leading to the Proposal of Moraxella canis sp. nov. for Moraxella catawhalis-Like Strains GEERT JANNES,' MARIO VANEECHOU'TTE,2 MARTINE LA"O0,3 MONIQUE GILLIS,3 MARC VANCANNEYT,3 PETER VANDAMME,3 GERDA VERSCHRAEGEN,2 HUGO VAN HEUVERSWYN,l AND RUDI ROSSAU1* Innogenetics N.I?, Industriepark Zwijnaarde 7, Box 4, B-9052 Ghent, ' and Department of Medical Microbiology, Universitair Ziekenhuis, and Laboratorium voor Microbiologie, University of Ghent, B-9000 Ghent, Belgium The taxonomic position of a group of 16 Moruxellu catamhulk-like strains, isolated mainly from dogs, was examined by using morphological tests, biochemical tests, serology, ribotyping with oligonucleotide probes, polymerase chain reaction typing of the 16s rRNA gene and the 16s-23s rRNA gene spacer region, polyacrylamide gel electrophoresis of total proteins, fatty acid profiles, moles percent G+C, dot spot and in-solution DNA-DNA hybridizations, and DNA-rRNA hybridizations. It was found that these organisms constitute a distinct cluster within the genus Moraxelca. Since they differ genotypically as well as phenotypically from previously described Moraxeh species, a new species, Moruxella canis, is proposed to accommodate these isolates. The type strain is LMG 11194 (= N7 = CCUG 8415A). During the testing of the specificity and reliability of DNA selected on the basis of different criteria. Strain ATCC 25238 probes for the detection of Moraxella catarrhalis, some is the type strain. Strains D6, J9, and W3 are lipopolysac- strains presumptively identified as M.
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