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Ion Channels Expression and Function Are Strongly Modified in Solid Tumors and Vascular Malformations

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Ion Channels Expression and Function Are Strongly Modified in Solid Tumors and Vascular Malformations Biasiotta et al. J Transl Med (2016) 14:285 DOI 10.1186/s12967-016-1038-y Journal of Translational Medicine RESEARCH Open Access Ion channels expression and function are strongly modified in solid tumors and vascular malformations Antonella Biasiotta1†, Daniela D’Arcangelo2†, Francesca Passarelli2, Ezio Maria Nicodemi2* and Antonio Facchiano2* Abstract Background: Several cellular functions relate to ion-channels activity. Physiologically relevant chains of events lead- ing to angiogenesis, cell cycle and different forms of cell death, require transmembrane voltage control. We hypothe- sized that the unordered angiogenesis occurring in solid cancers and vascular malformations might associate, at least in part, to ion-transport alteration. Methods: The expression level of several ion-channels was analyzed in human solid tumor biopsies. Expression of 90 genes coding for ion-channels related proteins was investigated within the Oncomine database, in 25 independ- ent patients-datasets referring to five histologically-different solid tumors (namely, bladder cancer, glioblastoma, melanoma, breast invasive-ductal cancer, lung carcinoma), in a total of 3673 patients (674 control-samples and 2999 cancer-samples). Furthermore, the ion-channel activity was directly assessed by measuring in vivo the electrical sym- pathetic skin responses (SSR) on the skin of 14 patients affected by the flat port-wine stains vascular malformation, i.e., a non-tumor vascular malformation clinical model. Results: Several ion-channels showed significantly increased expression in tumors (p < 0.0005); nine genes (namely, CACNA1D, FXYD3, FXYD5, HTR3A, KCNE3, KCNE4, KCNN4, CLIC1, TRPM3) showed such significant modification in at least half of datasets investigated for each cancer type. Moreover, in vivo analyses in flat port-wine stains patients showed a significantly reduced SSR in the affected skin as compared to the contralateral healthy skin (p < 0.05), in both latency and amplitude measurements. Conclusions: All together these data identify ion-channel genes showing significantly modified expression in different tumors and cancer-vessels, and indicate a relevant electrophysiological alteration in human vascular mal- formations. Such data suggest a possible role and a potential diagnostic application of the ion–electron transport in vascular disorders underlying tumor neo-angiogenesis and vascular malformations. Keywords: Cancer, Ion-channels, Autonomic nervous system, Sympathetic skin response, SSR, Flat port-wine stains Background transmembrane voltage represents a fundamental pro- Several key cellular functions are related to transmem- cess in many physiologically relevant steps, includ- brane potentials and lie under the control of ion chan- ing cell cycle progression [1] and different forms of cell nels, pumps and gap junction complexes. Controlling death [2, 3]. Over expression or increased activity of ion channels has been demonstrated as a response to mito- gens exposure [4–6]. Several studies show a direct link *Correspondence: [email protected]; [email protected] †Antonella Biasiotta and Daniela D’Arcangelo contributed equally to this between the transmembrane ion flow and carcinogenesis work [7, 8]. However, as pointed out [9], the role membrane 2 Istituto Dermopatico dell’Immacolata, IDI-IRCCS, Fondazione Luigi Maria potential plays in the pathogenesis of several disorders, Monti, via Monti di Creta 104, 00167 Rome, Italy Full list of author information is available at the end of the article including cancer, is still not well understood. Plasma © 2016 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Biasiotta et al. J Transl Med (2016) 14:285 Page 2 of 15 membrane de-polarization has a pivotal role at different we thus hypothesized that ion-transport may represent stages of cell cycle progression and in various cell types a measurable sign of the altered underlying angiogenesis. [9]. Namely, endothelial cells hyper-polarization has We, therefore, measured in vivo the ion-channel func- been shown to contribute to cell division arrest [10], and tion in a human vascular malformation model, namely channels are known to control migratory cellular prop- flat port-wine stains, as a model to test electrical-stimuli erties in wound healing [11]. Further, Ca2+, K+ and Cl− transport in a human vascular disorder accepted by the channels are essential regulators of cell proliferation and Ethic Committee. cancer development [12–16]. As recently further dem- onstrated, several ion-channels are directly involved in Methods controlling tumor—[17] as well as non-tumor angiogen- Ion‑channel gene expression investigation esis [18]. Expression and activity of TRPV4 channel have Gene expression levels were investigated by accessing been found suppressed in tumor endothelium [19], and human cancer datasets available at Oncomine (http:// its activation has been found to normalize tumor vessels www.oncomine.org). The current Oncomine version [20]; inhibiting Cl− channel activity has anti-angiogenesis contains several hundred different patients-datasets, and anti-glioma properties [21]; finally, human voltage- referring to tumors biopsies obtained from almost any dependent K+ channel has been found associated with histological source; unfortunately vascular tumors (such cancer aggressiveness and angiogenesis [22]. Therefore, as hemangioma, angiomas, hemangioendothelioma, ion-channels play a fundamental role in cancer progres- angiosarcoma) are lacking from such database. In the sion as well as in angiogenesis. current study 25, independent datasets from histologi- Flat port-wine stains are non-tumor malformations of cally different solid tumors were investigated, namely, the skin capillaries [23]. Cutaneous capillary malforma- bladder cancer, glioblastoma, melanoma, breast cancer, tions are usually isolated finding. However, they may lung adenocarcinoma, as indicated in details in Table 1. occasionally coexist with cerebral or ocular vascular mal- Table 2 reports the detailed list of the 90 ion-channels formations, constituting the rare sporadic neurocutane- and ion-channel related genes investigated in the pre- ous Sturge-Weber syndrome (SWS) affecting the cephalic sent study. Briefly, different members were selected from microvasculature, or may represent signs of more aggres- 21 channel families, namely: amiloride-sensitive cation sive vascular malformations or even vascular tumors. channels, calcium Channels voltage-dependent, cation The sympathetic skin response (SSR) is an alteration channels sperm associated, FXYD domain containing ion in skin electrical potential evoked by strong stimuli; it transport regulators, gamma-aminobutyric acid (GABA) consists of a multineuronal reflex activated by a variety receptors, glutamate receptors ionotropic, potassium of afferent inputs where the efferent branches involve channels voltage gated subfamily, cholinergic receptors sympathetic sudomotor fibers. The electrodermal activ- (Nicotinic), chloride channels, cyclic nucleotide gated ity reflects sympathetic cholinergic sudomotor function channels, glutamate Receptors, sodium leak channels, which induces changes in skin resistance to electrical purinergic receptors P2X, sodium-hydrogen exchanger conduction. The response is mediated by ions flux via regulatory factor 4, regulatory solute carrier proteins, activation of receptor-coupled Ca2+, Cl− and K+ chan- sodium channels, glucose activated Ion channels, two nels [24]. Since SSR reflects peripheral C fibers activity, pore segment channels, transient receptor potential cat- it is considered a reliable quantitative measure of sym- ion channels, zinc activated ion channels, aquaporins. pathetic function and dysfunction as well as in polyneu- Expression of such 90 genes was evaluated in oncomine ropathies and dysautonomic disorders [25–27]. database by setting “cancer vs. normal analysis” and We have previously shown novel serum markers able choosing as cancer type: “superficial bladder cancer” to indicate cardiovascular diseases, [28, 29] as well as within the bladder cancer group, “glioblastoma” within soluble factors able to affect angiogenesis [30] and to dis- the brain and CNS cancer group, “breast invasive criminate infantile hemangioma from more aggressive ductal carcinoma” within the breast cancer group, “lung vascular malformations or tumors [31]. We also investi- adenocarcinoma” within the lung cancer group, and gated novel molecular markers of melanoma set-up and “melanoma”. progression [32]. Expression fold change (cancer vs. normal samples) and In the present study, we further addressed the issue p values were reported for each analysis. Gene expres- of altered angiogenesis in tumor and non-tumor condi- sion in tissue biopsies from 3673 patients was analyzed. tions. The expression level of 90 ion-channel genes was Namely, 674 control-samples and 2999 cancer-samples investigated in five different solid tumors, having a dif- were investigated. ferent histological origin. The expression
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