Geographic Variation in Morphological, Genetic and Clonal Diversity of The

Total Page:16

File Type:pdf, Size:1020Kb

Geographic Variation in Morphological, Genetic and Clonal Diversity of The Geographic variation in morphological, genetic and clonal diversity of the parasitoid wasp Pelecinus polyturator (Hymenoptera: Pelecinidae) By Victor Shegelski ENSC 489 Prepared for Dr. D. Hemmerling 04/04/2014 Abstract The wasp species Pelecinus polyturator, the pelecinid wasp, is a widely dispersed species that is most easily recognized by its abnormally long abdomen. The range of this organism’s habitat extends from Argentina to Canada making it subject to a wide range of weather and climatic conditions. This range in environmental variation could lead to adaptations including altered morphology and unorthodox reproductive techniques such as parthenogenesis. Samples have been collected from populations in Canada, the USA and Honduras and have been compared for geographic differences in morphology and genetic relatedness. Morphological comparisons show that: 1) Specimens from Canada and the USA are larger than Honduran specimens; 2) Honduran Specimens have relatively longer antennae; 3) American specimens have relatively longer abdomens; 4) Canadian specimens have less variance in wing size and antennae length. ISSR-PCR and agarose gel electrophoresis produced DNA bands based on the CA10 and GT10 primers that were used; however, the resolution was too low to be analysed for genetic relatedness. With methodological adjustments these primers are likely to produce significant results that can identify relatedness within and between populations. Keywords: Pelecinus polyturator, morphological variation, Darwin’s fecundity advantage model, Bergmann’s rule, selective pressure, climate change, climatic variation, population relatedness, thelytoky parthenogenesis, ISSR-PCR, Pelecinidae, Hymenoptera. Introduction Pelecinus polyturator (Drury) is an endoparasitoid wasp species that is primarily known for its incredibly long and thin abdomen with ovipositor as well as the parasitic larval stage in its life cycle (Brues, 1928). Sexual reproduction is rare due to low male representation in the species (Johnson and Musetti, 1999). P. polyturator compensates for the lack of sexual partners by performing thelytoky Shegelski 1 parthenogenesis (Brues, 1928); the female lays unfertilized eggs that produce clones of the mother. Extant species of these parasitoid wasps range from South Eastern Canada (as far West as Manitoba), through the eastern U.S.A. and the Honduran rain forests to Argentina (Johnson and Musetti, 1999). This study is designed to find relationships in the form of geographic trends in morphology, genetic diversity and clone presence in populations. Adult P.polyturator primarily use Phyllophaga spp. grubs as the host for their larvae (Johnson and Musetti, 1999); the wasp pierces the ground with its abdomen (up to 5 cm) in order to lay its eggs on beetle grubs, generally one per grub (Aguiar, 1997). Once the eggs on the grubs hatch, the parasitic wasp larvae enter the Phyllophagas host. The larvae feed and develop within the host and emerge to pupate; they eventually go through complete metamorphosis to emerge as an adult and, once an adult, the wasp feeds on nectar (Shih et al, 2009). The rarity of males is proportional to latitudinal positioning; Northern populations will have very few males (Aguiar, 1997) whereas the male presence in equatorial populations tends to be greater (Masner, 1995). Parthenogenesis is a form of reproduction in areas populated with males but the sexual reproduction also diversifies the clonal base of the community, as Vorburger (2006) found when studying parthenogenic aphids (Myzus persicae). One would expect lower genetic diversity in the Northern populations of P. polyturator as thelytoky parthenogenesis is the more common method of reproduction; however, Loxdale and Lushai (2003) found in some species (most prominently aphids) even cloned populations may still have a disparate gene pool by means of high mutation rates. With thelytokus and clonal diversity a species could easily colonize new locations and start new populations from a single individual (Loxdale and Lushai, 2003); P. polyturator have the largest distribution of the Pelecinidae (Johnson and Musetti, 1999) which may ultimately be due to their thelytoky parthenogenesis. Shegelski 2 Because of its vast geographic distribution, P. polyturator is subject to varying environmental conditions such as climate, food, host sources, predators and availability of sexual reproduction. It is likely that these varying conditions and pockets of sexual reproduction could lead to varying genetic diversity when measured geographically; this is especially possible considering there are gaps in the distribution of P.polyturator, effectively creating large ‘island’ populations (Johnson and Musetti, 1999). Female P. polyturator body length on average can range from 20-90mm and males average 15mm (Johnson and Musetti, 1999). A factor that could affect a size trend in P. polyturator may be host larvae size differences in which larger larvae lead to larger wasps (Johnson and Musetti, 1999); as mentioned above, host species of P. polyturator are primarily of the diverse genus Phyllophaga which are widely distributed and can also be found in the southern areas of Canada where P. polyturator exist (Galloway, 2008). The larvae of Phylophaga spp. can range from 15-25mm in length and current known larval hosts exploited by P. polyturator include Phyllophaga anxia (LeConte), P. inverse (Horn), P. drakei (Kirby), P. rugosa (Melsheimer) and Podischnus agenor (Olivier) (Johnson and Musetti 1999). In their research, Johnson and Musetti (1999) completed a large scale sample collection and comparison based on species variation, color and location collected. They also projected the regions of the Americas with an appropriate climate for the survival of P. polyturator . This area is much larger than its current dispersal and it has already been found thriving in areas even further north than what was projected (Galloway 2008) with no reported adverse effects on the ecosystem. This species is capable of adaptation that was never before predicted and the variety of grubs capable of acting as hosts for the pelecinid wasp is still not known. Clausen (1940) believed the original host of P. polyturator was actually a wood boring beetle similar to the mountain pine beetle (Dendroctonus ponderosae) currently ravaging the forests of Northern Alberta; given the climate projections by Johnson and Musetti (1999) it is possible that in the future this wasp could play a very beneficial role in Shegelski 3 agriculture and forestry as other parasitoid wasp species do today through biological pest control (Hoffmann and Frodsham 1993). Further analysis of size differences based on environmental factors and comparisons of genetic diversity have yet to be examined for P. polyturator. There are obvious differences in wing size between other parasitoid wasp species based upon flight requirements such as distance and power needed to get to food sources and hosts (Price, 1973); for example, if host populations are dense the wing size may be smaller as the energy is no longer required for long, frequent flights and would be best invested elsewhere. In Drosophila melanogaster, Frazer et al (2008) found that larger wing sizes can offer a biomechanical advantage in cold weather flying. It is possible the long thin abdomen with ovipositor could be more structurally robust in areas with heavy soil that requires more strength to get through to host grubs. In other Hymenoptera, specifically the Apidae species Bombus terrestris, or bumblebees, antennae length has been found to be related to olfactory sensitivity and detection threshold; longer antennae allow for more complex detection, and also lower the olfactory detection threshold (Spaethe et al 2007). Any consistent morphological variation between regions could reveal adaptive changes in the species, and it is possible that these regional adaptations could be the early stages of speciation through the founder effect (Templeton 1980). In order to examine genetic diversity intersimple sequence repeat-polymerase chain reaction (ISSR-PCR) was used as a form of genetic fingerprinting that targets base pair repetitions in the DNA of the P. polyturator specimens; this would help determine the clonal nature of geographic populations. In this case ISSR-PCR was used because there were no previously determined genetic markers for any Pelecinus spp. using the Scopus, Jstor and Springer Link databases, and using microsatallite markers is efficient but species specific (Selkoe and Toonen 2006). Without prior research, microsatellite determination is beyond the scope of this study. Shegelski 4 Selection of ISSR-PCR primers was ultimately be based on testing with a slightly defined starting point. Luque et al (2002) wanted to find genetic variation between different noctuids (from the Order Lepidoptera); they found in the ISSR-PCR testing of 11 primers that (CA)10 was the most accurate given a consistent 300ng of DNA was used in each test. Nascimento et al (2010) and Santos et al (2011) both tested 93 primers from UBC primer set #9 when testing genetic diversity and structure in bees from the Hymenoptera order. In each study (CA)n primers were among those selected for the final analysis. Uesugi and Sato (2012) found 3 (CA)n primers, (CA)11, (CA)12 and (CA)15, where among the 9 most efficient in their study isolating microsatellite loci in Encarsia smithi, a parasitoid wasp from the family Aphelinidae (order: Hymenoptera). Lucas et al (2009) identified several (GT)n
Recommended publications
  • Morphology, Taxonomy, and Biology of Larval Scarabaeoidea
    Digitized by the Internet Archive in 2011 with funding from University of Illinois Urbana-Champaign http://www.archive.org/details/morphologytaxono12haye ' / ILLINOIS BIOLOGICAL MONOGRAPHS Volume XII PUBLISHED BY THE UNIVERSITY OF ILLINOIS *, URBANA, ILLINOIS I EDITORIAL COMMITTEE John Theodore Buchholz Fred Wilbur Tanner Charles Zeleny, Chairman S70.S~ XLL '• / IL cop TABLE OF CONTENTS Nos. Pages 1. Morphological Studies of the Genus Cercospora. By Wilhelm Gerhard Solheim 1 2. Morphology, Taxonomy, and Biology of Larval Scarabaeoidea. By William Patrick Hayes 85 3. Sawflies of the Sub-family Dolerinae of America North of Mexico. By Herbert H. Ross 205 4. A Study of Fresh-water Plankton Communities. By Samuel Eddy 321 LIBRARY OF THE UNIVERSITY OF ILLINOIS ILLINOIS BIOLOGICAL MONOGRAPHS Vol. XII April, 1929 No. 2 Editorial Committee Stephen Alfred Forbes Fred Wilbur Tanner Henry Baldwin Ward Published by the University of Illinois under the auspices of the graduate school Distributed June 18. 1930 MORPHOLOGY, TAXONOMY, AND BIOLOGY OF LARVAL SCARABAEOIDEA WITH FIFTEEN PLATES BY WILLIAM PATRICK HAYES Associate Professor of Entomology in the University of Illinois Contribution No. 137 from the Entomological Laboratories of the University of Illinois . T U .V- TABLE OF CONTENTS 7 Introduction Q Economic importance Historical review 11 Taxonomic literature 12 Biological and ecological literature Materials and methods 1%i Acknowledgments Morphology ]* 1 ' The head and its appendages Antennae. 18 Clypeus and labrum ™ 22 EpipharynxEpipharyru Mandibles. Maxillae 37 Hypopharynx <w Labium 40 Thorax and abdomen 40 Segmentation « 41 Setation Radula 41 42 Legs £ Spiracles 43 Anal orifice 44 Organs of stridulation 47 Postembryonic development and biology of the Scarabaeidae Eggs f*' Oviposition preferences 48 Description and length of egg stage 48 Egg burster and hatching Larval development Molting 50 Postembryonic changes ^4 54 Food habits 58 Relative abundance.
    [Show full text]
  • An Annotated Checklist of Wisconsin Scarabaeoidea (Coleoptera)
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Center for Systematic Entomology, Gainesville, Insecta Mundi Florida March 2002 An annotated checklist of Wisconsin Scarabaeoidea (Coleoptera) Nadine A. Kriska University of Wisconsin-Madison, Madison, WI Daniel K. Young University of Wisconsin-Madison, Madison, WI Follow this and additional works at: https://digitalcommons.unl.edu/insectamundi Part of the Entomology Commons Kriska, Nadine A. and Young, Daniel K., "An annotated checklist of Wisconsin Scarabaeoidea (Coleoptera)" (2002). Insecta Mundi. 537. https://digitalcommons.unl.edu/insectamundi/537 This Article is brought to you for free and open access by the Center for Systematic Entomology, Gainesville, Florida at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Insecta Mundi by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. INSECTA MUNDI, Vol. 16, No. 1-3, March-September, 2002 3 1 An annotated checklist of Wisconsin Scarabaeoidea (Coleoptera) Nadine L. Kriska and Daniel K. Young Department of Entomology 445 Russell Labs University of Wisconsin-Madison Madison, WI 53706 Abstract. A survey of Wisconsin Scarabaeoidea (Coleoptera) conducted from literature searches, collection inventories, and three years of field work (1997-1999), yielded 177 species representing nine families, two of which, Ochodaeidae and Ceratocanthidae, represent new state family records. Fifty-six species (32% of the Wisconsin fauna) represent new state species records, having not previously been recorded from the state. Literature and collection distributional records suggest the potential for at least 33 additional species to occur in Wisconsin. Introduction however, most of Wisconsin's scarabaeoid species diversity, life histories, and distributions were vir- The superfamily Scarabaeoidea is a large, di- tually unknown.
    [Show full text]
  • Biological Control of Insect Pests in Puerto Rico1 Fernando Gallardo-Covas2 J
    Biological control of insect pests in Puerto Rico1 Fernando Gallardo-Covas2 J. Agrie. Univ. P.R. 101:153-163 (2017) ABSTRACT The first documented observation on biological control of insects in Puerto Rico was made by botanist Andres Pedro Ledru who arrived on the island in 1797 with a commission of French scientists. Much later (1880- 1887), Johannes Gundlach classified braconid wasps of the genus Conura. In 1895, Fernando López-Tuero published a treatise on sugarcane and the beneficial effects of Apantelessp. and Euplectrussp., two natural enemies of the sugarcane borer Diatraea saccharalis F. Since then more than 75 natural enemies were introduced, some very effective like the toad Bufo marinus L. in controlling white grubs in sugarcane, and others partially effective like the coffee leafminer parasitoid, Mi rax insularis Muesebeck. Also, natural enemies were exported from Puerto Rico to other countries as far away as Mauritania. However, with the advent of chlorinated insecticides derived from DDT, in the early 1950s, the work on biocontrol declined dramatically, and not until the mid-1980s was biological control again considered a remedy to the problem of pests. In Puerto Rico, research was focused more on recognizing the endemic fauna of natural enemies and the introduction of parasitoids/ predators. This paper presents a historical review and discussion of trends and approaches to the biological control of pests from its beginnings to the present. Key words: entomology, Puerto Rico, biological control, parasitoids, predators RESUMEN Control biológico de los insectos plaga en Puerto Rico: Revisión bibliográfica La primera observación documentada sobre control biológico de insectos en Puerto Rico fue hecha por el botánico Andrés Pedro Ledrú, quien llegó con una comisión de científicos franceses en el 1797.
    [Show full text]
  • Quick Guide for the Identification Of
    Quick Guide for the Identification of Maryland Scarabaeoidea Mallory Hagadorn Dr. Dana L. Price Department of Biological Sciences Salisbury University This document is a pictorial reference of Maryland Scarabaeoidea genera (and sometimes species) that was created to expedite the identification of Maryland Scarabs. Our current understanding of Maryland Scarabs comes from “An Annotated Checklist of the Scarabaeoidea (Coleoptera) of Maryland” (Staines 1984). Staines reported 266 species and subspecies using literature and review of several Maryland Museums. Dr. Price and her research students are currently conducting a bioinventory of Maryland Scarabs that will be used to create a “Taxonomic Guide to the Scarabaeoidea of Maryland”. This will include dichotomous keys to family and species based on historical reports and collections from all 23 counties in Maryland. This document should be cited as: Hagadorn, M.A. and D.L. Price. 2012. Quick Guide for the Identification of Maryland Scarabaeoidea. Salisbury University. Pp. 54. Questions regarding this document should be sent to: Dr. Dana L. Price - [email protected] **All pictures within are linked to their copyright holder. Table of Contents Families of Scarabaeoidea of Maryland……………………………………... 6 Geotrupidae……………………………………………………………………. 7 Subfamily Bolboceratinae……………………………………………… 7 Genus Bolbocerosoma………………………………………… 7 Genus Eucanthus………………………………………………. 7 Subfamily Geotrupinae………………………………………………… 8 Genus Geotrupes………………………………………………. 8 Genus Odonteus...……………………………………………… 9 Glaphyridae..............................................................................................
    [Show full text]
  • Trapping Phyllophaga Spp. (Coleoptera: Scarabaeidae: Melolonthinae) in the United States and Canada Using Sex Attractants Paul S
    University of Rhode Island DigitalCommons@URI Plant Sciences and Entomology Faculty Plant Sciences and Entomology Publications 2006 Trapping Phyllophaga spp. (Coleoptera: Scarabaeidae: Melolonthinae) in the United States and Canada Using Sex Attractants Paul S. Robbins Steven R. Alm University of Rhode Island, [email protected] See next page for additional authors Creative Commons License This work is licensed under a Creative Commons Attribution 3.0 License. Follow this and additional works at: https://digitalcommons.uri.edu/pls_facpubs Citation/Publisher Attribution Robbins, P. S., Alm, S. R., Armstrong, C. D., Averill, A. L., Baker, T. C., Bauernfiend, R. J., ...Villani, M. G. (2006). Trapping Phyllophaga, spp. (Coleoptera: Scarabaeidae: Melolothinae) in the United States and Canada using sex attractants. Journal of Insect Science, 6, Article 39. Available at: http://dx.doi.org/10.1673/2006_06_39.1 This Article is brought to you for free and open access by the Plant Sciences and Entomology at DigitalCommons@URI. It has been accepted for inclusion in Plant Sciences and Entomology Faculty Publications by an authorized administrator of DigitalCommons@URI. For more information, please contact [email protected]. Authors Paul S. Robbins, Steven R. Alm, and et al This article is available at DigitalCommons@URI: https://digitalcommons.uri.edu/pls_facpubs/1 Trapping Phyllophaga spp. (Coleoptera: Scarabaeidae: Melolonthinae) in the United States and Canada using sex attractants Author(s): Paul S. Robbins, Steven R. Alm, CharlesD. Armstrong, Anne L. Averill, Thomas C. Baker, Robert J. Bauernfiend, Frederick P. Baxendale, S. Kris Braman, Rick L. Brandenburg, Daniel B. Cash, Gary J. Couch, Richard S. Cowles, Robert L.
    [Show full text]
  • Crop Profile for Corn in Michigan
    Crop Profile for Corn in Michigan Prepared Feb, 2002 General Production Information ● Michigan ranked 11th nationally in 2000 for corn grain production (44). ● Michigan contributed 2.4% to the total US production of corn in 2000 (44). ● The total acreage planted in Michigan was 2,200,000 acres in 2000, down 100,000 acres from 1998 (44). ● Total grain corn production for Michigan in 2000 was 244,280,000 bushels, down 4% from 1999 (44). ● Grain corn harvested in 2000 for Michigan was 1,97,000 acres (44). ● Silage corn harvested in 2000 for Michigan was 225,000 acres with an average yield of 14 tons per acre (44). ● Corn grain production was valued at $612 million in 1997, $432.3 million in 1998, $451 million in 1999 and 464 million in 2000 (44). ● The average bushels/acre was 117 in 1997, 111 in 1998, 130 bushels in 1999, and 124 bushels in 2000 (43, 44). ● Corn continued to be Michigan's number one crop in value of production (44). PRODUCTION REGIONS: Corn is Michigan's number one crop in both acreage planted and value of production. The top five counties in corn production in 2000 were Huron, St Joseph, Lenawee, Sanilac and Saginaw. In 1998 they were Huron, Sanilac, Clinton, Ionia and Allegan counties and in 1999 Huron, Saginaw, Sanilac, Tuscola and Lenawee counties (43, 44). The Crop Profile/PMSP database, including this document, is supported by USDA NIFA. Cultural Practices Corn can be grown on most soils in Michigan but does best on well drained soils. Soils classified as poorly drained are also suitable for corn production if they are tile drained.
    [Show full text]
  • Biosecurity Risk Assessment
    An Invasive Risk Assessment Framework for New Animal and Plant-based Production Industries RIRDC Publication No. 11/141 RIRDCInnovation for rural Australia An Invasive Risk Assessment Framework for New Animal and Plant-based Production Industries by Dr Robert C Keogh February 2012 RIRDC Publication No. 11/141 RIRDC Project No. PRJ-007347 © 2012 Rural Industries Research and Development Corporation. All rights reserved. ISBN 978-1-74254-320-8 ISSN 1440-6845 An Invasive Risk Assessment Framework for New Animal and Plant-based Production Industries Publication No. 11/141 Project No. PRJ-007347 The information contained in this publication is intended for general use to assist public knowledge and discussion and to help improve the development of sustainable regions. You must not rely on any information contained in this publication without taking specialist advice relevant to your particular circumstances. While reasonable care has been taken in preparing this publication to ensure that information is true and correct, the Commonwealth of Australia gives no assurance as to the accuracy of any information in this publication. The Commonwealth of Australia, the Rural Industries Research and Development Corporation (RIRDC), the authors or contributors expressly disclaim, to the maximum extent permitted by law, all responsibility and liability to any person, arising directly or indirectly from any act or omission, or for any consequences of any such act or omission, made in reliance on the contents of this publication, whether or not caused by any negligence on the part of the Commonwealth of Australia, RIRDC, the authors or contributors. The Commonwealth of Australia does not necessarily endorse the views in this publication.
    [Show full text]
  • A Guide to Arthropods Bandelier National Monument
    A Guide to Arthropods Bandelier National Monument Top left: Melanoplus akinus Top right: Vanessa cardui Bottom left: Elodes sp. Bottom right: Wolf Spider (Family Lycosidae) by David Lightfoot Compiled by Theresa Murphy Nov 2012 In collaboration with Collin Haffey, Craig Allen, David Lightfoot, Sandra Brantley and Kay Beeley WHAT ARE ARTHROPODS? And why are they important? What’s the difference between Arthropods and Insects? Most of this guide is comprised of insects. These are animals that have three body segments- head, thorax, and abdomen, three pairs of legs, and usually have wings, although there are several wingless forms of insects. Insects are of the Class Insecta and they make up the largest class of the phylum called Arthropoda (arthropods). However, the phylum Arthopoda includes other groups as well including Crustacea (crabs, lobsters, shrimps, barnacles, etc.), Myriapoda (millipedes, centipedes, etc.) and Arachnida (scorpions, king crabs, spiders, mites, ticks, etc.). Arthropods including insects and all other animals in this phylum are characterized as animals with a tough outer exoskeleton or body-shell and flexible jointed limbs that allow the animal to move. Although this guide is comprised mostly of insects, some members of the Myriapoda and Arachnida can also be found here. Remember they are all arthropods but only some of them are true ‘insects’. Entomologist - A scientist who focuses on the study of insects! What’s bugging entomologists? Although we tend to call all insects ‘bugs’ according to entomology a ‘true bug’ must be of the Order Hemiptera. So what exactly makes an insect a bug? Insects in the order Hemiptera have sucking, beak-like mouthparts, which are tucked under their “chin” when Metallic Green Bee (Agapostemon sp.) not in use.
    [Show full text]
  • Adaptations of Insects at Cloudbridge Nature Reserve, Costa Rica
    Adaptations of Insects at Cloudbridge Nature Reserve, Costa Rica Aiden Vey Cloudbridge Nature Reserve July 2007 Introduction Costa Rica’s location between North and South America, its neotropical climate and variety of elevations and habitats makes it one of the biodiversity hotspots of the world. Despite being only 51,100km² in size, it contains about 5% (505,000) of the world’s species. Of these, 35,000 insect species have been recorded and estimates stand at around 300,000. The more well known insects include the 8,000 species of moth and 1,250 butterflies - almost 10% of the world total, and 500 more than in the USA! Other abundant insects of Costa Rica include ants, beetles, wasps and bees, grasshoppers and katydids. The following article presents a select few aspects of the insect life found at Cloudbridge, a nature reserve in the Talamanca mountain range. Relationships Insects play many important roles in Costa Rica, including pollination of the bountiful flora and as a food supply for many other organisms. The adult Owl butterfly (Caligo atreus, shown at right) feeds on many Heliconiaceae and Musaceae (banana) species, in particular on the rotting fruit. They are pollinators of these plants, but also use the leaves to lay eggs on. When hatched, the larvae remain on the plant and eat the leaves. Being highly gregarious, they can cause significant damage, and are considered as pests (especially in banana plantations). However, there are a number of insects that parasitise the Caligo larvae, including the common Winthemia fly (left) and Trichogramma and Ichneumon wasps, which act as biological control agents.
    [Show full text]
  • Relations and Distribution of Phyllophaga Harris (Lachnosterna Hope) in Temperate North America
    THE UNIVERSITY OF ILLINOIS LIBRARY RELATIONS AND DISTRIBUTION OF PHYLLOPHAGA HARRIS (LACHNOSTERNA HOPE) IN TEMPERATE NORTH AMERICA BY ROBERT DOUGLAS GLASGOW A. B. University of Illinois 1908. THESIS Submitted in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY IN ENTOMOLOGY IN THE GRADUATE SCHOOL OF THE UNIVERSITY OF ILLINOIS 1913 Digitized by the Internet Archive in 2014 http://archive.org/details/relationsdistribOOglas «\5 UNIVERSITY OF ILLINOIS THE GRADUATE SCHOOL May 17, 1913 190 1 HEREBY RECOMMEND THAT THE THESIS PREPARED UNDER MY SUPERVISION BY Robert Douglass Glasgow ENTITLED On the Evolution and Dispersal of the genus Phylloph - aga Haris ( =Lachnos terna Hope.) BE ACCEPTED AS FULFILLING THIS PART OF THE REQUIREMENTS FOR THE DEGREE OF Doctor of Philosophy Recommendation concurred in: Committee on v C^fc&t^c Final Examination 247383 i LIST OF PLATES Plate 1. Diagrammatic figure, showing relations of the various parts of the male genital tube in Col eopt era. Fusca-Hugosa Ser ies = Plates II-XII. Plate II. Phyllophaga hirticula Knoch. Male genital structures. Type specimen. Eastern form. Plate III. Phyllophaga hirticula Knoch. Western form. Plate IV. Phyllophaga hornii Smith. Male genital structures. Type specimen. Plate V. Phy llophaga profunda blanchard. Male genital structures. Prom type of P. biimpressa Smith. Plate VI. Phyllophaga rugosa Melsheimer. Male genital structures. Type lost. From form designated by LeConte and by Horn. Plate VII. Phyllophaga f rat erna Harris. Male genital structures. Compared with type specimen. Plate Vlli. Phyllophaga delata Horn. Male genital structures. Type specimen. Plate IX. Phyll ophaga inf delis Horn. Male genital structures.
    [Show full text]
  • Cylindera Cursitans (Ant-Like Tiger Beetle) Resembles Swift Tiger Beetle—Marking Connected, Legs Not Metallic
    5/13/2015 Antlions & tiger beetles & woolly bears, oh my! Ted C. MacRae 30 April 2015 A local entomologist’s look at some of Missouri’s more interesting insects All photos by Ted C. MacRae unless noted otherwise Who Am I? Grew up in Kansas City (yes, I’m a city boy!) B.S. Agriculture 1979, University of Missouri M.S. Entomology 1981, University of Missouri 1982–1990: Missouri Department of Agriculture, St. Louis, MO 1990–1995: Novo Nordisk Entotech, Davis, CA 1995–present: Monsanto Company, Chesterfield, MO First photo by Christopher Brown 1 5/13/2015 Discovering Entomology: collected insects as hobby during childhood, then seriously starting in college Photos by unknown (L), Carol Zimmermann (R) 2 5/13/2015 I’m never happier than when I am studying insects… Photos (clockwise from upper left) by Chuck Bellamy, unknown, Rich Thoma, & self …or photographing them Photos by (clockwise from upper left) Kent Fothergill, Rich Thoma, Lisa Ruschke , unknown 3 5/13/2015 Missouri: where forest yields to prairie The Natural Divisions of Missouri Source: R. H. Thom & J. H. Wilson. 1980. The Natural Divisions of Missouri. Trans. MO Acad. Sci. 14:9–23. 4 5/13/2015 Insects in the Glaciated Plains Insects in the Loess Hills Snakeweed grasshopper, Hesperotettix viridis Prairie May beetle, Phyllophaga lanceolata Common milkweed beetle, Tetraopes tetrophthalmus 5 5/13/2015 Desmocerus palliatus (Coleoptera: Cerambycidae) Associated with Sambucus canadensis (elderberry) Hypsithermal relicts Relicts from hypsithermal period that began 9,000 yrs ago
    [Show full text]
  • On the Occurrence of Pelecinus Polyturator (Hymenoptera: Pelecinidae) in Manitoba, Canada
    Proceedings of the Entomological Society of Manitoba, Volume 64, 2008 On the occurrence of Pelecinus polyturator (Hymenoptera: Pelecinidae) in Manitoba, Canada Terry D. Galloway Department of Entomology University of Manitoba Winnipeg, Manitoba R3T 2N2 [email protected] Pelecinus polyturator (Drury) is a striking, jet black wasp that is endoparasitic in June beetle larvae, Phyllophaga spp. in North, Central and South America (Ham- mond 1944, Lim et al. 1980, Johnson and Musetti 1999). The behaviour of this wasp is poorly known, with limited observations on host location by Davis (1919) and Bennett (2003) and on mating behaviour (Aguiar 1997). Hammond (1944) reported that 1-3% of Phyllophaga larvae were parasitized in Ontario and Québec. In 1999, Johnson and Musetti revised the genus Pelecinus Latreille, and provided extensive information on the distribution and biology of P. polyturator, the most widely distrib- uted species in the genus. They obtained specimens for their study from a number of museums in Canada and the United States, and on that basis, plotted the distribution of the specimens they obtained, mostly from the eastern United States and southern Ontario, with scattered records from Central America, the central United States and Atlantic provinces (Fig. 7B, Johnson and Musetti 1999). They also provided a map to describe a climatic model with potential distribution for P. polyturator in North America (Fig. 7A, Johnson and Musetti 1999). Johnson and Musetti (1999) did not obtain any specimens of P. polyturator from Manitoba from any of the museums they contacted, nor did their climatic model include Manitoba even within the range of marginal climate for this species.
    [Show full text]