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Rasamsonia Composticola, a New Thermophilic Species Isolated from Compost in Yunnan, China

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Rasamsonia Composticola, a New Thermophilic Species Isolated from Compost in Yunnan, China Mycol Progress DOI 10.1007/s11557-012-0827-9 ORIGINAL ARTICLE Rasamsonia composticola, a new thermophilic species isolated from compost in Yunnan, China Yuan-Ying Su & Lei Cai Received: 3 March 2012 /Revised: 3 May 2012 /Accepted: 10 May 2012 # German Mycological Society and Springer 2012 Abstract Rasamsonia composticola sp. nov. is described, have a maximum growth temperature at or above 50 °C and illustrated, and compared with similar taxa. This species a minimum temperature of growth at or above 20 °C are produces globose to ellipsoid ascomata, spherical asci borne considered thermophilic, and those that have a thermal in short chains and globose, 1-celled ascospores, typical of maximum near 50 °C and a minimum well below 20 °C Rasamsonia. Anamorph on CYA, MEA, and PDA produces are thermotolerant (Cooney and Emerson 1964). Taxonom- verrucose, rough-walled conidiophores, and hyaline and ically, thermophiles constitute a heterogeneous group cylindrical conidia. This novel species is thermophilic with (Mouchacca 1997, 2007; Salar and Aneja 2007). Thermo- optimal growth temperature of 45–50 °C, and minimum philic fungi are important natural bio-resources capable of growth temperature of 30 °C. Phylogenetic analyses based producing thermo-stable enzymes, which are industrially on combined ITS rDNA, partial calmodulin, and β-tubulin important (Maheshwari et al. 2000). sequences, and combined partial RPB2, Tsr1, and Cct8 gene Houbraken et al. (2012) introduced Rasamsonia, with the sequences were conducted. Both confirmed the generic type species R. emersonii based on a polyphasic study of placement in Rasamsonia and showed its close phylogenetic thermotolerant and thermophilic species in Trichocomaceae. relationships to several species in the genus, such as R. Rasamsonia species are morphologically characterized by emersonii and R. byssochlamydoides. olive-brown ascomata containing spherical, evanescent asci borne in short chains, and penicillate anamorphs with rough- Keywords Calmodulin . Morphology . Phylogeny . walled conidiophores (Houbraken et al. 2012). The com- Talaromyces . β-tubulin bined dataset of partial RNA polymerase II gene (RPB2), putative ribosome biogenesis protein gene (Tsr1) and puta- tive chaperonin complex component TCP-1 gene (Cct8) Introduction sequences showed that Rasamsonia species form a distinct clade within the Trichocomaceae (Houbraken et al. 2012). Temperature is an environmental factor that plays a decisive Currently, the genus Rasamsonia comprises six species, of role in the distribution, diversity, growth and survival of which five were transferred from Talaromyces or Geosmi- microorganisms in ecosystems. Only a few fungi have the thia, and one was newly described (Houbraken et al. 2012). ability to thrive at temperaturew between 45 and 55 °C Several species of the genus have been commercialized; for (Maheshwari et al. 2000). These fungi have been generally example, a thermostable extracellular enzyme from R. emer- categorized as thermophilic and thermotolerant fungi sonii has been used in the wheat-baking process (Waters et (Cooney and Emerson 1964; Mouchacca 2007). Fungi that al. 2010). The objective of this paper is to characterize a novel : species of Rasamsonia isolated from compost in Yunnan, Y.-Y. Su L. Cai (*) China. The combined partial RPB2, Tsr1, and Cct8 gene State Key Laboratory of Mycology, Institute of Microbiology, sequences were adopted, based on the study of Houbraken Chinese Academy of Sciences, Beijing 100101, People’s Republic of China et al. (2012), to confirm the correct generic identification. e-mail: [email protected] ITS-rDNA gene (ITS), partial calmodulin (Cmd), and β- Mycol Progress Table 1 Strains used in phylogenetic analysis of selected Eurotiales CBS no. Name Other collections GenBank accession no. (RPB2/Tsr1/Cct8) or reference1 CBS 513.88 Aspergillus clavatusa NRRL 1 (0 ATCC 10070CBS 513.650IMI 15949) Houbraken et al. (2012) Aspergillus flavusa NRRL 3357 (0 CBS 1282020ATCC 200026) Houbraken et al. (2012) Aspergillus fumigatusa Af293 Houbraken et al. (2012) Aspergillus nigera Houbraken et al. (2012) Aspergillus terreusa NIH 2624 Houbraken et al. (2012) CBS 100.11NT Byssochlamys nivea ATCC 22260 JF417414; JF417381; JF4174514 CBS 101075HT Byssochlamys spectabilis ATCC 90900 JF417446; JF417412; JF4174546 CBS 295.48IsoT Coccidioides immitisa Strain “RS” Houbraken et al. (2012) Emericella nidulansa FGSC A4 (0 ATCC 381630CBS 112.46) Houbraken et al. (2012) Hamigera avellanea ATCC 104140IMI 0402300NRRL 1938 JF417424; JF417391; JF4174524 Penicillium chrysogenuma Wisconsin 54-1255 Houbraken et al. (2012) CBS 139.45NT Penicillium citrinum ATCC 1109 0 IMI 091961 0 MUCL JF417416; JF417383; JF4174516 29781 0 NRRL 1841 CBS 344.61IsoT Penicillium crustaceum ATCC 18240 0 IMI 086561 0 MUCL JF417428; JF417395; JF4174528 2685 0 NRRL 3332 CBS 325.48 Penicillium expansum ATCC 7861 0 IBT 5101 0 IMI 039761 0 MUCL JF417427; JF417394; JF4174527 29192 0 NRRL 976 CBS 125543NT Penicillium glabrum IBT 22658 0 IMI 91944 JF417447; JF417413; JF4174547 CBS 353.48NT Penicillium namyslowskii ATCC 11127 0 IMI 040033 0 MUCL JF417430; JF417397; JF4174530 29226 0 NRRL 1070 CBS 101.69T Rasamsonia argillacea DTO 97E4 0 IMI 156096 0 IBT 31199 JF417415; JF417382; JF4174515 CBS 128785T Rasamsonia brevistipitata DTO 25H2 0 IBT 31187 JN406530; JN406523; N406520 CBS 413.71T Rasamsonia byssochlamydoides DTO 149D6 0 IBT 11604 JF417437; JF417403; JF4174537 Rasamsonia composticola CGMCC 3.13669T JQ729684; JQ729686; JQ729682 Rasamsonia composticola CGMCC3.14946 JQ729685; JQ729687; JQ729683 CBS 275.58NT Rasamsonia cylindrospora DTO 138F8 0 IBT 31202 0 ATCC JF417423; JF417390; JF4174523 18223 0 IMI 071623 CBS 100538T Rasamsonia eburnea DTO 105D6 0 IBT 17519 JN406532; JN406524; JN406521 CBS 393.64T Rasamsonia emersonii DTO 48I1 0 IBT 21695 0 ATCC JF417434; JF417401; JF4174534 16479 0 IMI 116815 0 IMI 116815ii CBS 398.69 Sagenomella diversispora JF417435; JF417402; JF4174536 CBS 426.67 Sagenomella griseoviridis ATCC 18505 0 IMI 113160 JF417438; JF417404; JF4174538 CBS 427.67IsoT Sagenomella humicola ATCC 18506 0 IMI 113166 JF417439; JF417405; JF4174539 CBS 429.67IsoT Sagenomella striatispora ATCC 18510 0 IMI 113163 JF417440; JF417406; JF4174540 CBS 296.48HT Talaromyces bacillisporus ATCC 10126 0 IMI 040045 0 NRRL 1025 JF417425; JF417392; JF4174525 CBS 100536HT Talaromyces emodensis IBT 14990 JF417445; JF417411; JF4174545 CBS 310.38NT Talaromyces flavus IMI 197477 0 NRRL 2098 JF417426; JF417393; JF4174526 CBS 398.68HT Talaromyces leycettanus ATCC 22469 0 IMI 178525 JF417435; JF417402; JF4174535 CBS 348.51NT Talaromyces luteus CECT 2950 0 IMI 089305 JF417429; JF417396; JF4174529 CBS 371.87 Talaromyces luteus JF417431; JF417398; JF4174531 Talaromyces marneffeia ATCC 18224 (CBS 334.59 0 IMI 68794) Houbraken et al. (2012) CBS 642.68NT Talaromyces mineoluteum IMI 089377 0 MUCL 28666 JF417443; JF417409; JF4174543 Talaromyces stipitatusa ATCC 10500 (0 NRRL 1006 0 CBS 375.48 0 IMI 39805) Houbraken et al. (2012) CBS 252.87HT Talaromyces viridis IMI 288716 JF417422; JF417389; JF4174522 CBS 373.48 Talaromyces trachyspermus ATCC 10497 0 IMI 040043 0 NRRL 1028 JF417432; JF417399; JF4174532 CBS 391.48NT Talaromyces wortmanii ATCC 10517 0 IMI 040047 0 NRRL 1017 JF417433; JF417400; JF4174533 CBS 891.70 Thermoascus aurantiacus IMI 173037 JF417444; JF417410; JF4174544 CBS 181.67T Thermoascus crustaceus ATCC 16462 0 IMI 126333 JF417417; JF417384; JF4174517 CBS 528.71NT Thermoascus thermophilus IMI 123298 0 NRRL 5208 JF417442; JF417408; JF4174542 CBS 218.34 Thermomyces lanuginosus MUCL 8338 JF417418; JF417385; JF4174518 Mycol Progress Table 1 (continued) CBS no. Name Other collections GenBank accession no. (RPB2/Tsr1/Cct8) or reference1 CBS 224.63 Thermomyces lanuginosus MUCL 8337 JF417419; JF417386; JF4174519 CBS 236.58HT Thermomyces thermophilus ATCC 10518 0 IMI 048593 0 NRRL 2155 JF417420; JF417387; JF4174520 CBS 247.57 Trichocoma paradoxa MUCL 39666 0 IBT 31159 JF417421; JF417388; JF4174521 CBS 512.65NT Warcupiella spinulosa ATCC 16919 0 IMI 075885 0 NRRL 4376 JF417441; JF417407; JF4174541 CBS culture collection of the CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands (WDCM 133); DTO internal culture collection of CBS-KNAW Fungal Biodiversity Centre; IMI CABI Genetic Resources Collection, Surrey, UK (WDCM 214); IBT culture collection of the Center for Microbial Biotechnology (CMB) at Department of Systems Biology, Technical University of Denmark (WDCM 758); NRRL ARS Culture Collection, U.S. Department of Agriculture, Peoria, Illinois, USA (WDCM 97); ATCC American Type Culture Collection, Manassas, VA, USA (WDCM 1); MUCL Mycotheque de l'Universite catholique de Louvain, Louvain-la-Neuve, Belgium (WDCM 308). WDCM WFCC-World Data Centre for Microorganisms a Sequences derived from published genome sequences tubulin (TUB2) regions were also sequenced and analyzed lock plastic bags and returned to the laboratory, where 10 g of to infer its phylogenetic relationships. each sample were added into a shake flask with 90 mL sterile water, and shaken at 120 rpm for 30 min at 45 °C. The extract was diluted to a series of concentrations, i.e. 10−2,10−3,10−4, − − − − Materials and methods 10 5,106,107,and108, and a 0.2-mL extract from each concentration was spread onto potato-dextrose agar (PDA) Isolation, cultural and morphological characterization containing ampicillin (100 μg/mL) and streptomycin (100 μg/mL) (3 replicates). All plates were incubated at Composts made from rice straw and cow dung were collected 45 °C for 3–10 days with
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