Author Manuscript Published OnlineFirst on December 4, 2014; DOI: 10.1158/1541-7786.MCR-14-0372 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Running Title: Role of SULFs in colorectal cancer cells Original Article Enhanced Tumorigenic Potential of Colorectal Cancer Cells by Extracellular Sulfatases Carolina M Vicente1, Marcelo A Lima1,2, Edwin A Yates2,1, Helena B Nader1, Leny Toma1* 1Disciplina de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de São Paulo, UNIFESP, Brazil 2Institute of Integrative Biology, Department of Biochemistry, University of Liverpool, Liverpool, UK *Corresponding author: Leny Toma, Disciplina de Biologia Molecular, Universidade Federal de São Paulo, UNIFESP, Rua Três de Maio, 100 – 4º andar, Vila Clementino, CEP 04044-020, São Paulo, SP, Brazil. E-mail:
[email protected],
[email protected], Tel (+55) (11) 55793175; FAX (+55) (11) 55736407. Disclosure of Potential Conflicts of Interest No potential conflicts of interest were disclosed by the authors. Downloaded from mcr.aacrjournals.org on September 24, 2021. © 2014 American Association for Cancer Research. Author Manuscript Published OnlineFirst on December 4, 2014; DOI: 10.1158/1541-7786.MCR-14-0372 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Abstract Heparan sulfate endosulfatase-1 and -2 (SULF1 and SULF2) are two important extracellular 6-O-endosulfatases that remove 6-O sulfate groups of N-glucosamine along heparan sulfate (HS) proteoglycan chains often found in the extracellular matrix (ECM). The HS sulfation pattern influences signaling events at the cell surface, which are critical for interactions with growth factors and their receptors.