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Under the Direction of R. Michael Roe
ABSTRACT DONOHUE, KEVIN VINCENT. Genomics of Tick Reproduction and Development. (Under the direction of R. Michael Roe). The major hemelipoglyco-carrier protein (CP) found throughout the development of male and female adult American dog ticks, Dermacentor variabilis (Say) was sequenced. DvCP is a single transcript coding for two protein subunits that together contain three motifs—(a) a lipoprotein n-terminal domain that is a common attribute of proteins that bind lipids, carbohydrates and metals, (b) a domain of unknown function characteristic of proteins with several large open beta sheets and (c) a von Willebrand factor type D domain near the carboxy-terminus apparently important for multimerization. These motifs also found in tick vitellogenin are not shared by heme-binding proteins studied thus far in other hematophagous insects. DvCP message was highest in fat body and salivary gland but was also found in midgut and ovary. Expression was initiated by blood feeding in virgin females and not by mating typical of tick vitellogenin (Vg); and the message was found in fed males at levels similar to part fed, virgin females. CP appears to be highly conserved among the Ixodida and shares a common origin with Vg. In the second part of this study, the tick synganglion transcriptome was studied by pyrosequencing to identify neuropeptides that regulate reproduction and development. Here we characterize fourteen putative neuropeptides (allatostatin, insulin-like peptide, ion-transport peptide, sulfakinin, bursicon alpha/beta, eclosion hormone, glycoprotein hormone alpha/beta, corazonin, four orcokinins) and five neuropeptide receptors (gonadotropin receptor, leucokinin-like receptor, sulfakinin receptor, calcitonin receptor, pyrokinin receptor) from the synganglion of female American dog ticks. -
Highlights in the Knowledge of Brown Spider Toxins
Chaves-Moreira et al. Journal of Venomous Animals and Toxins including Tropical Diseases (2017) 23:6 DOI 10.1186/s40409-017-0097-8 REVIEW Open Access Highlights in the knowledge of brown spider toxins Daniele Chaves-Moreira1, Andrea Senff-Ribeiro1, Ana Carolina Martins Wille1,2, Luiza Helena Gremski1, Olga Meiri Chaim1 and Silvio Sanches Veiga1* Abstract Brown spiders are venomous arthropods that use their venom for predation and defense. In humans, bites of these animals provoke injuries including dermonecrosis with gravitational spread of lesions, hematological abnormalities and impaired renal function. The signs and symptoms observed following a brown spider bite are called loxoscelism. Brown spider venom is a complex mixture of toxins enriched in low molecular mass proteins (4–40 kDa). Characterization of the venom confirmed the presence of three highly expressed protein classes: phospholipases D, metalloproteases (astacins) and insecticidal peptides (knottins). Recently, toxins with low levels of expression have also been found in Loxosceles venom, such as serine proteases, protease inhibitors (serpins), hyaluronidases, allergen-like toxins and histamine-releasing factors. The toxin belonging to the phospholipase-D family (also known as the dermonecrotic toxin) is the most studied class of brown spider toxins. This class of toxins single-handedly can induce inflammatory response, dermonecrosis, hemolysis, thrombocytopenia and renal failure. The functional role of the hyaluronidase toxin as a spreading factor in loxoscelism has also been demonstrated. However, the biological characterization of other toxins remains unclear and the mechanism by which Loxosceles toxins exert their noxious effects is yet to be fully elucidated. The aim of this review is to provide an insight into brown spider venom toxins and toxicology, including a description of historical data already available in the literature. -
Comparative Analyses of Venoms from American and African Sicarius Spiders That Differ in Sphingomyelinase D Activity
This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier’s archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright Author's personal copy Toxicon 55 (2010) 1274–1282 Contents lists available at ScienceDirect Toxicon journal homepage: www.elsevier.com/locate/toxicon Comparative analyses of venoms from American and African Sicarius spiders that differ in sphingomyelinase D activity Pamela A. Zobel-Thropp*, Melissa R. Bodner 1, Greta J. Binford Department of Biology, Lewis and Clark College, 0615 SW Palatine Hill Road, Portland, OR 97219, USA article info abstract Article history: Spider venoms are cocktails of toxic proteins and peptides, whose composition varies at Received 27 August 2009 many levels. Understanding patterns of variation in chemistry and bioactivity is funda- Received in revised form 14 January 2010 mental for understanding factors influencing variation. The venom toxin sphingomyeli- Accepted 27 January 2010 nase D (SMase D) in sicariid spider venom (Loxosceles and Sicarius) causes dermonecrotic Available online 8 February 2010 lesions in mammals. Multiple forms of venom-expressed genes with homology to SMase D are expressed in venoms of both genera. -
ANA CAROLINA MARTINS WILLE.Pdf
UNIVERSIDADE FEDERAL DO PARANÁ ANA CAROLINA MARTINS WILLE AVALIAÇÃO DA ATIVIDADE DE FOSFOLIPASE-D RECOMBINANTE DO VENENO DA ARANHA MARROM (Loxosceles intermedia) SOBRE A PROLIFERAÇÃO, INFLUXO DE CÁLCIO E METABOLISMO DE FOSFOLIPÍDIOS EM CÉLULAS TUMORAIS. CURITIBA 2014 i Wille, Ana Carolina Martins Avaliação da atividade de fosfolipase-D recombinante do veneno da aranha marrom (Loxosceles intermedia) sobre a proliferação, influxo de cálcio e metabolismo de fosfolipídios em células tumorais Curitiba, 2014. 217p. Tese (Doutorado) – Universidade Federal do Paraná – UFPR 1.veneno de aranha marrom. 2. fosfolipase-D. 3.proliferação celular. 4.metabolismo de lipídios. 5.influxo de cálcio. ANA CAROLINA MARTINS WILLE AVALIAÇÃO DA ATIVIDADE DE FOSFOLIPASE-D RECOMBINANTE DO VENENO DA ARANHA MARROM (Loxosceles intermedia) SOBRE A PROLIFERAÇÃO, INFLUXO DE CÁLCIO E METABOLISMO DE FOSFOLIPÍDIOS EM CÉLULAS TUMORAIS. Tese apresentada como requisito à obtenção do grau de Doutor em Biologia Celular e Molecular, Curso de Pós- Graduação em Biologia Celular e Molecular, Setor de Ciências Biológicas, Universidade Federal do Paraná. Orientador(a): Dra. Andrea Senff Ribeiro Co-orientador: Dr. Silvio Sanches Veiga CURITIBA 2014 ii O desenvolvimento deste trabalho foi possível devido ao apoio financeiro do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação Araucária e SETI-PR. iii Dedico este trabalho àquela que antes da sua existência foi o grande sonho que motivou minha vida. Sonho que foi a base para que eu escolhesse uma profissão e um trabalho. À você, minha amada filha GIOVANNA, hoje minha realidade, dedico todo meu trabalho. iv Dedico também este trabalho ao meu amado marido, amigo, professor e co- orientador Dr. -
Loxosceles Laeta
Parasitología Artículo Original Desarrollo de cohortes y parámetros poblacionales de la araña del rincón Loxosceles laeta Mauricio Canals y Rigoberto Solís Universidad de Chile, Santiago, Development and population parameters of cohorts of the Chilean Chile. Facultad de Medicina, recluse spider Loxosceles laeta Departamento de Medicina (Oriente) (MC). Background: Despite the abundant eco-epidemiological knowledge of the Chilean reclusive spider, Loxosceles Facultad de Ciencias Veterinarias y laeta, which causes all forms of loxoscelism in Chile, the main characteristics of this species its stages of develop- Silvoagropecuarias, Departamento ment remains poorly known especially in the medical area. Objective: In this study we address these issues with de Ciencias Biológicas Animales the goal of providing clear images of the development of this species and for the first time on population projec- (RS). tions as well as the relationship between mature and immature instars, useful data for the control and prevention Recibido: 5 de marzo de 2014 of accidental bites. Results: We found that L. laeta is an r-selected species, with R0 = 2.1, a generation time of Aceptado: 17 de julio de 2014 G = 2.1 years, with a concentration of the reproductive value of females between the first and second year of life. We determined the average sizes and development times of all instars. The first vary between 2.3 mm at birth and Correspondencia a: about 13 mm at adulthood. The total development time was about 1 year. Discussion: The population projection Mauricio Canals Lamabarri [email protected] by Leslie matrix suggested great capacity for growth and dispersal with clear seasonal population fluctuations associated with reproduction. -
Arthropod Envenomation
Arthropod Envenomation Michael R. Loomis, DVM, MA, DACZM North Carolina Zoological Park Hymenoptera Envenomation Order Hymenoptera Family Vespidae- wasps Family Formicidae- ants Familt Mutillidae- velvet ants Family Apidae- bees • Stinger is a modified ovipositor Bee and Wasp Venom Components • Proteins, peptides and • Apitoxin – 52% Melitten (potent anti- amines inflammatory agent that – Phospholipase increases production of cortisol) – Histamine – 10-12% Phospholipase A2 – Bradykinin – 2-5% Aldolapin (blocks – cyclooxygenase) Acetylcholine – 1-3% Hyuronidase – Dopamine – 0.5-2% Histamine – Seratonin – 1-2% Dopamine and noradrenaline – Mast cell degranulating – 2% Protease-inhibitors peptide – Apamine increases cortisol – Mastoparan production, mild neurotoxin Ant Venom Components • Fire ants- 95% alkaloid (Unique among ants) • Most other ants, similar to bee and wasp venom • Harvester ant venom contains a hemolysin Venom Toxicity Family Common Name LD 50 (mg/kg) Apidae Honey bee 2.8 Mutillidae Velvet ant 71.0 Vespidae Paper wasp 2.4 Vespidae Yellowjacket 3.5 Formicidae Harvester ant 0.66 Formicidae Maricopa Harvester ant 0.12 Morbidity and Mortality Bees and Wasps • In US, 9.3 million ant • 17-56% produce local stings and 1 million reactions stings of other • Hymenoptera/year 1-2% produce generalized reactions • More deaths/year than any other type of • 5% seek medical care envenomation • 30-120 deaths from • Most deaths are the wasp and bee result of Anaphylaxis stings/year Local Reactions • Pain • Edema which may extend 10 cm from -
Niche Modelling of the Chilean Recluse Spider Loxosceles Laeta and Araneophagic Spitting Spider Scytodes Globula and Risk for Loxoscelism in Chile
Medical and Veterinary Entomology (2016) 30, 383–391 doi: 10.1111/mve.12184 Niche modelling of the Chilean recluse spider Loxosceles laeta and araneophagic spitting spider Scytodes globula and risk for loxoscelism in Chile M. CANALS1, A. TAUCARE-RIOS2, A. D. BRESCOVIT3, F.PEÑA-GOMEZ2,G.BIZAMA2, A. CANALS1,4, L. MORENO5 andR. BUSTAMANTE2 1Departamento de Medicina and Programa de Salud Ambiental, Escuela de Salud Pública, Facultad de Medicina, Universidad de Chile, Santiago, Chile, 2Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile, 3Laboratório Especial de Coleções Zoológicas, Instituto Butantan, São Paulo, Brazil, 4Dirección Académica, Clínica Santa Maria, Santiago, Chile and 5Departamento de Zoología, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile Abstract. In Chile, all necrotic arachnidism is attributed to the Chilean recluse spider Loxosceles laeta (Nicolet) (Araneae: Sicariidae). It is predated by the spitting spider Scytodes globula (Nicolet) (Araneae: Scytodidae). The biology of each of these species is not well known and it is important to clarify their distributions. The aims of this study are to elucidate the variables involved in the niches of both species based on environmental and human footprint variables, and to construct geographic maps that will be useful in estimating potential distributions and in defining a map of estimated risk for loxoscelism in Chile. Loxosceles laeta was found to be associated with high temperatures and low rates of precipitation, whereas although S. globula was also associated with high temperatures, its distribution was associated with a higher level of precipitation. The main variable associated with the distribution of L. -
Loxosceles Laeta (Nicolet) (Arachnida: Araneae) in Southern Patagonia
Revista de la Sociedad Entomológica Argentina ISSN: 0373-5680 ISSN: 1851-7471 [email protected] Sociedad Entomológica Argentina Argentina The recent expansion of Chilean recluse Loxosceles laeta (Nicolet) (Arachnida: Araneae) in Southern Patagonia Faúndez, Eduardo I.; Alvarez-Muñoz, Claudia X.; Carvajal, Mariom A.; Vargas, Catalina J. The recent expansion of Chilean recluse Loxosceles laeta (Nicolet) (Arachnida: Araneae) in Southern Patagonia Revista de la Sociedad Entomológica Argentina, vol. 79, no. 2, 2020 Sociedad Entomológica Argentina, Argentina Available in: https://www.redalyc.org/articulo.oa?id=322062959008 PDF generated from XML JATS4R by Redalyc Project academic non-profit, developed under the open access initiative Notas e recent expansion of Chilean recluse Loxosceles laeta (Nicolet) (Arachnida: Araneae) in Southern Patagonia La reciente expansión de Loxosceles laeta (Nicolet) (Arachnida: Araneae) en la Patagonia Austral Eduardo I. Faúndez Laboratorio de entomología, Instituto de la Patagonia, Universidad de Magallanes, Chile Claudia X. Alvarez-Muñoz Unidad de zoonosis, Secretaria Regional Ministerial de Salud de Aysén, Chile Mariom A. Carvajal [email protected] Laboratorio de entomología, Instituto de la Patagonia, Universidad de Magallanes, Chile Catalina J. Vargas Revista de la Sociedad Entomológica Argentina, vol. 79, no. 2, 2020 Laboratorio de entomología, Instituto de la Patagonia, Universidad de Sociedad Entomológica Argentina, Magallanes, Chile Argentina Received: 06 February 2020 Accepted: 03 May 2020 Published: 29 June 2020 Abstract: e recent expansion of the Chilean recluse Loxosceles laeta (Nicolet, 1849) Redalyc: https://www.redalyc.org/ in southern Patagonia is commented and discussed in the light of current global change. articulo.oa?id=322062959008 New records are provided from both Región de Aysén and Región de Magallanes. -
Brown Recluse Spider, Loxosceles Reclusa Gertsch & Mulaik (Arachnida: Araneae: Sicariidae)1 G
EENY299 Brown Recluse Spider, Loxosceles reclusa Gertsch & Mulaik (Arachnida: Araneae: Sicariidae)1 G. B. Edwards2 Introduction Kansas, east through middle Missouri to western Tennessee and northern Alabama, and south to southern Mississippi. The brown recluse spider, Loxosceles reclusa Gertsch & Gorham (1968) added Illinois, Kentucky, and northern Mulaik, is frequently reported in Florida as a cause of Georgia. Later, he added Nebraska, Iowa, Indiana and necrotic lesions in humans. For example, in the year 2000 Ohio, with scattered introductions in other states, includ- alone, Loft (2001) reported that the Florida Poison Control ing Florida; his map indicated a record in the vicinity of Network had recorded nearly 300 alleged cases of brown Tallahassee (Gorham 1970). recluse bites in the state; a subset of 95 of these bites was reported in the 21 counties (essentially Central Florida) under the jurisdiction of the regional poison control center in Tampa. I called the Florida Poison Control Network to confirm these numbers, and was cited 182 total cases and 96 in the Tampa region. The actual numbers are less important than the fact that a significant number of unconfirmed brown recluse spider bites are reported in the state every year. Yet not one specimen of brown recluse spider has ever been collected in Tampa, and the only records of Loxosceles species in the entire region are from Orlando and vicinity. A general review of the brown recluse, along with a critical examination of the known distribution of brown recluse and related spiders in Florida, seems in order at this time. Figure 1. Female brown recluse spider, Loxosceles reclusa Gertsch & Distribution Mulaik. -
Sharing the Space: Coexistence Among Terrestrial Predators in Neotropical Caves
Journal of Natural History ISSN: 0022-2933 (Print) 1464-5262 (Online) Journal homepage: http://www.tandfonline.com/loi/tnah20 Sharing the space: coexistence among terrestrial predators in Neotropical caves L.P.A. Resende & M.E. Bichuette To cite this article: L.P.A. Resende & M.E. Bichuette (2016): Sharing the space: coexistence among terrestrial predators in Neotropical caves, Journal of Natural History, DOI: 10.1080/00222933.2016.1193641 To link to this article: http://dx.doi.org/10.1080/00222933.2016.1193641 Published online: 04 Jul 2016. Submit your article to this journal Article views: 2 View related articles View Crossmark data Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=tnah20 Download by: [CAPES] Date: 07 July 2016, At: 10:53 JOURNAL OF NATURAL HISTORY, 2016 http://dx.doi.org/10.1080/00222933.2016.1193641 Sharing the space: coexistence among terrestrial predators in Neotropical caves L.P.A. Resende and M.E. Bichuette Laboratório de Estudos Subterrâneos, Departamento de Ecologia e Biologia Evolutiva, Universidade Federal de São Carlos, São Carlos, Brazil ABSTRACT ARTICLE HISTORY The subterranean environment has a set of unique characteristics, Received 3 February 2015 including low thermic variation, high relative humidity, areas with Accepted 20 May 2016 total absence of light and high dependence on nutrient input KEYWORDS from the epigean environment. Such characteristics promote dis- Competition; predation; tinct ecological conditions that support the existence of unique niche segregation; spatial communities. In this work, we studied seven caves in the distribution; subterranean Presidente Olegário municipality, Minas Gerais state, Southeast environment Brazil, to determine their richness of predatory species, to under- stand how they are spatially distributed in the cave and whether their distribution is influenced by competition and/or predation. -
Taxa Names List 6-30-21
Insects and Related Organisms Sorted by Taxa Updated 6/30/21 Order Family Scientific Name Common Name A ACARI Acaridae Acarus siro Linnaeus grain mite ACARI Acaridae Aleuroglyphus ovatus (Troupeau) brownlegged grain mite ACARI Acaridae Rhizoglyphus echinopus (Fumouze & Robin) bulb mite ACARI Acaridae Suidasia nesbitti Hughes scaly grain mite ACARI Acaridae Tyrolichus casei Oudemans cheese mite ACARI Acaridae Tyrophagus putrescentiae (Schrank) mold mite ACARI Analgidae Megninia cubitalis (Mégnin) Feather mite ACARI Argasidae Argas persicus (Oken) Fowl tick ACARI Argasidae Ornithodoros turicata (Dugès) relapsing Fever tick ACARI Argasidae Otobius megnini (Dugès) ear tick ACARI Carpoglyphidae Carpoglyphus lactis (Linnaeus) driedfruit mite ACARI Demodicidae Demodex bovis Stiles cattle Follicle mite ACARI Demodicidae Demodex brevis Bulanova lesser Follicle mite ACARI Demodicidae Demodex canis Leydig dog Follicle mite ACARI Demodicidae Demodex caprae Railliet goat Follicle mite ACARI Demodicidae Demodex cati Mégnin cat Follicle mite ACARI Demodicidae Demodex equi Railliet horse Follicle mite ACARI Demodicidae Demodex folliculorum (Simon) Follicle mite ACARI Demodicidae Demodex ovis Railliet sheep Follicle mite ACARI Demodicidae Demodex phylloides Csokor hog Follicle mite ACARI Dermanyssidae Dermanyssus gallinae (De Geer) chicken mite ACARI Eriophyidae Abacarus hystrix (Nalepa) grain rust mite ACARI Eriophyidae Acalitus essigi (Hassan) redberry mite ACARI Eriophyidae Acalitus gossypii (Banks) cotton blister mite ACARI Eriophyidae Acalitus vaccinii -
US EPA, Pesticide Product Label, CSI 16-119A L-N-P CS,04/26/2021
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY WASHINGTON, DC 20460 OFFICE OF CHEMICAL SAFETY AND POLLUTION PREVENTION April 26, 2021 Lisa Adamson Regulatory Manager Control Solutions 5903 Genoa-Red Bluff Pasadena, TX 77507-1041 Subject: PRIA Label Amendment – Add efficacy claims against mosquitoes Product Name: CSI 16-119A L-N-P CS EPA Registration Number: 53883-427 Application Dates: 3/6/2020, 3/12/21, 4/22/2021 Decision Numbers: 566783, 572300, 573378 Dear Ms. Adamson: The application referred to above, submitted under the Federal Insecticide, Fungicide and Rodenticide Act, as amended is acceptable under FIFRA sec 3 (c)(5). You must submit and/or cite all data required for registration/reregistration/registration review of your product when the Agency requires all registrants of similar products to submit such data. A stamped copy of your labeling is enclosed for your records. This labeling supersedes all previously accepted labeling. You must submit one (1) copy of the final printed labeling before you release the product for shipment with the new labeling. In accordance with 40 CFR 152.130(c), you may distribute or sell this product under the previously approved labeling for 18 months from the date of this letter. After 18 months, you may only distribute or sell this product if it bears this new revised labeling or subsequently approved labeling. “To distribute or sell” is defined under FIFRA section 2(gg) and its implementing regulation at 40 CFR 152.3. Should you wish to add/retain a reference to the company’s website on your label, then please be aware that the website becomes labeling under the Federal Insecticide Fungicide and Rodenticide Act and is subject to review by the Agency.