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13C Glucose Qualitative Flux Analysis in Hepg2 Cells

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13C Glucose Qualitative Flux Analysis in Hepg2 Cells Application Note Metabolomics 13C Glucose Qualitative Flux Analysis in HepG2 cells Using an Agilent 6546 LC/Q-TOF and VistaFlux Authors Abstract Siriluck Wattanavanitchakorn The Agilent 6546 LC/Q-TOF is designed to simultaneously provide excellent and Sarawut Jitrapakdee isotope ratio fidelity, wide dynamic range, and high mass resolution independent Department of Biochemistry, of acquisition speed. Flux analysis requires the combination of isotope ratio Faculty of Science, fidelity, high resolution, and dynamic range for good results. When combined with Mahidol University, Agilent MassHunter VistaFlux software, a comprehensive workflow from data Bangkok, Thailand acquisition to data analysis is provided to help scientists perform qualitative flux Israr Ansari, analysis in a streamlined fashion. VistaFlux software is composed of tools for Melissa J. Longacre, and feature extraction, analysis of isotope incorporation and isotopologue abundance, Michael J. MacDonald natural abundance correction, statistical analysis, and visualization of metabolic Children’s Diabetes Center, pathways. University of Wisconsin This Application Note demonstrates qualitative flux analysis in human School of Medicine and Public carcinoma cell lines using U-13C glucose as the tracer. Data were acquired on Health, Madison, WI, USA a 6546 LC/Q-TOF system, and analyzed with MassHunter VistaFlux. The study Mahmoud El Azzouny and demonstrates the effect of pyruvate carboxylase knockdown on glucose flux in the James Pyke TCA cycle in HepG2 cells. Agilent Technologies, Inc. Santa Clara, CA, USA Introduction VistaFlux software provides full data replaced with 2 mL of DMEM containing analysis and visualization of flux data. 10 mM 13C-labeled glucose, and cells Stable isotope labeling provides a unique This full workflow solution was applied to were incubated for 30 minutes at 37 °C. picture of intracellular metabolism. pyruvate carboxylase knockdown HepG2 Experiments were done in triplicate for Although metabolomics profiling cells treated with U-13C glucose. each cell line. provides a great overview of the Pyruvate carboxylase (PC) is a abundance of different metabolites, Metabolite extraction mitochondrial anaplerotic enzyme that many metabolic changes do not result After a quick washing with 150 mM generates citric acid cycle intermediates in an accumulation or a reduction at the ammonium acetate, cells were extracted for biosynthetic purposes. This enzyme metabolite level. Stable isotope tracing with 8:1:1 (v/v/v) methanol: chloroform: is essential for gluconeogenesis in can provide insightful information water, followed by sonication and liver and kidney, de novo lipogenesis in not revealed by normal metabolomic centrifugation. The clear supernatant adipose tissue, and glucose-induced profiling. In stable isotope tracing, was then transferred to HPLC vials and insulin secretion in pancreatic beta isotopologues are normalized to each injected. cells. Recent studies show that PC is other to calculate the enrichment pivotal for several cancer forms where LC/MS analysis percentage. This self-normalizing it supports various anabolic pathways. approach makes stable isotope tracer Data were acquired using We have previously shown that PC studies a powerful option to probe a 6546 LC/Q-TOF coupled to suppression reduces the proliferation metabolic changes in challenging an Agilent 1290 Infinity II LC. rate in highly invasive breast cancer biological systems that might differ in Chromatographic separation was cells as well as reducing glucose and performed on an Agilent InfinityLab cell number. 1 glutamine anaplerosis . This Application Poroshell 120 HILIC-Z, 2.1 × 100 mm, There are many challenges to accurately Note shows that PC inhibition exhibits a 2.7 μm (p/n 675775-924), with a identify small but significant isotope similar effect on human hepatic cancer UHPLC Guard, HILIC-Z, 2.1 mm × changes that alter normal metabolite cells (HepG2). 5 mm, 2.7 µm (p/n 821725-947). A 10× isotope distribution. The mass mobile phase buffered stock solution spectrometer (MS) should maintain Experimental (100 mM ammonium acetate, pH accurate isotope abundance and mass 9.0) was first prepared in water, then measurements for correct metabolite Cell culture and reagents final mobile phases were prepared identification and quantitation in Pyruvate carboxylase knockdown HepG2 with the addition of either water (A) complex samples. In addition, the MS cells were prepared using siRNA, as or acetonitrile (ACN, B). To ensure a should simultaneously provide a wide described previously1. Five different constant concentration during gradient dynamic range and high resolution. A knockdown cell lines were generated elution, the InfinityLab deactivator wide dynamic range accommodates with different levels of inhibition additive (p/n 5191-4506) was added varying metabolite concentrations and (PC179, PC2096, PC 3436, and PC847). to both aqueous and organic mobile isotope incorporation rates while high Approximately 3 × 106 cells were plated phases. Pooled samples and blank resolution reduces interference from into a 60 mm culture plate. Cells were injections were analyzed to ensure coeluting compounds. Finally, the data cultured in Dulbecco’s modified Eagle’s chromatographic stability2. analysis software should correctly assign medium (DMEM) supplemented with isotopologues and perform natural 10 % (v/v) fetal bovine serum (FBS), Software packages isotope correction to provide an accurate 100 units/mL penicillin, and 100 μg/mL • MassHunter Acquisition software net label incorporation. streptomycin, and were maintained at version 10.0 The 6546 LC/Q-TOF and VistaFlux 37 °C with 5 % CO2 for two days. The • Agilent Profinder V10.0 software provide a comprehensive medium was replaced with complete • Omix Premium V1.9.30 workflow for qualitative flux analysis. DMEM containing 3 mM glucose, and The 6546 LC/Q-TOF simultaneously cells were cultured for one more day. • PCDL Manager B.08 delivers the isotope ratio fidelity, wide On the day of the experiment, cells were • Agilent MassHunter Pathways to dynamic range, and high mass resolution incubated for 30 minutes with 0 mM PCDL software B.08 required for qualitative flux analysis. glucose DMEM, then the medium was 2 Table 1. LC and MS parameters. Parameter Value MS Conditions LC Conditions MS System 6546 Q-TOF LC/MS InfinityLab Poroshell 120 HILIC-Z, 2.1 × 100 mm, 2.7 μm Ionization Source Agilent Jet Stream Column (p/n 675775-924) with a UHPLC Guard, HILIC-Z, 2.1 mm × Polarity Negative 5 mm, 2.7 µm (p/n 821725-947) Gas Temperature 200 °C A) 10 mM Ammonium acetate in water, pH 9 with 5 µm deactivator additive Drying Gas 10 L/min Mobile Phase (p/n 5191-4506) Nebulizer Pressure 40 psi B) 10 mM Ammonium acetate in water/ACN 10:90 (v:v), pH 9 with 5 µm deactivator additive (p/n 5191-4506) Sheath Gas Temperature 300 °C Flow Rate 0.25 mL/min Sheath Gas Flow 12 L/min 0 to 2 minutes 90 %B Capillary Voltage 3,000 V 2 to 12 minutes 90 to 60 %B Nozzle Voltage 0 V Gradient 12 to 15 minutes 60 %B 15 to 16 minutes 60 to 90 %B Fragmentor 125 V 16 to 24 minutes 90 %B Skimmer 65 V Column Temperature 25 °C Octopole 1 RF Voltage 750 V Injection Volume 1 µL Acquisition Range m/z 50 to 1,000 Autosampler Temperature 6 °C Reference Mass m/z 980.01638 and 119.0363 Data analysis PCDL with retention time Pathway to PCDL Using Agilent MassHunter Pathways Metabolites in PCDL will have: This tool will export all metabolites in the to PCDL software, an Agilent Personal • A chemical formula to generate the pathway of choice, together with their Compound Database and Library file right isotope pattern masses and identifier to a PCDL format. (.cdb) was created for the tricarboxylic • An identifier ID such as METLIN, acid (TCA) cycle drawn from BioCyc. HMDB, or KEGG, and a CAS number to In addition, pyruvate, aspartate, and allow correct mapping to the metabolic pathway. glutamate were added to the custom • A retention time, to avoid false PCDL. Metabolite retention times were identification, especially in complex added to the custom PCDL, which was samples. The retention time can be then used as the database for batch updated if needed. isotopologue extraction in Profinder. Results from Profinder were exported for visualization in Omix Premium software. Profinder Omix Premium Figure 1 summarizes an overview of the • Profinder uses the metabolite formulas • Omix Premium provides an easy way to data analysis workflow. in the PCDL to extract the isotopologues visualize isotopologue data on a chosen for a given metabolite. metabolic pathway. • Isotopologues for a given metabolite • Pathways of choice are first selected, must have the correct mass and then Profinder results are automatically retention time as well as the same mapped to the pathways. chromatographic peak shape. • Sample group information is imported • Natural isotope correction is performed with the Profinder results. automatically to provide the net labeling. • Results can be exported to Omix Premium. Figure 1. Overview of VistaFlux data analysis workflow. 3 Results and discussion ×106 133.0142 1.1 42,350 1.0 The flux samples were analyzed on the 0.9 6546 LC/Q-TOF MS. Figure 2 shows a 0.8 0.7 representative MS spectrum for malate 136.0243 0.6 41,549 isotopologues with greater than 40,000 Counts 0.5 135.0209 resolution while showing less than 5 % 0.4 41,076 0.3 134.0176 CV for all isotopologues among seven 0.2 41,493 137.0276 technical replicates (Figure 3). The high 0.1 38,643 0 resolution of the 6546 Q-TOF reduces 133.0 133.5 134.0 134.5 135.0 135.5 136.0 136.5 137.0 potential interferences, increasing Mass-to-charge (m/z) confidence in the data analysis. The Figure 2. Mass spectra of malate isotopologues with m/z and resolution labeled on each ion peak. excellent reproducibility of the isotope ratios enables the detection of low 1.2 Replicate 1 incorporation rates in a qualitative flux Replicate 2 experiment.
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