DOCSLIB.ORG

Vegetarian Substitutes for Gelatin Soft Capsules

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Vegetarian Substitutes for Gelatin Soft Capsules As appeared in January 2020 Tablets & Capsules Copyright CSC Publishing www.tabletscapsules.com capsules Vegetarian substitutes for gelatin soft Claudia Silva capsules Procaps Kirti Vatsa Beroe This article provides an overview of the current state of materi- oft capsules, also called softgels, are one of the most als and technology used to produce vegetarian soft capsules widely used solid oral dosage forms, particularly in the and discusses applications, processing, and suitable products nutraceutical market. Traditional softgels are made from Sgelatin, but interest in vegetarian alternatives to gelatin for these alternatives to soft gelatin capsules. soft capsules (GSCs) is growing. The materials used to make vegetarian soft capsules (VSCs) have very different properties than gelatin. The first commercially viable VSCs appeared on the market around 2001 and were developed for encapsulating oils Copyright CSC Publishing and suspensions for dietary and cosmetic products [1]. derived from animals, is prone to price fluctuations, and The capsules were made from carrageenan-modified has a relatively large carbon footprint. starch (C-MS), and the encapsulation equipment sub- VSCs have faced some concerns from consumers jected the capsules to a curing step, in which the capsules interested in “clean label” products—defined as products passed through a band of hot air. Later machines included containing only unprocessed or unmodified ingredi- die rolls designed to increase the thickness of the seal and ents—since starches are chemically modified materials. prevent the capsule contents from leaking. Also, carrageenan suffered from a The industry has made continuous long period of concerns that it was car- improvements to the materials, equip- Vegetarian soft cinogenic, causing many food produc- ment, and processes involved in their ers, especially in the US, to stop using manufacture. As a result, vegetarian soft capsules can now be it in their products. However, the FDA capsules can now be produced using considers carrageenan a generally recog- the same type of mixing and encapsula- produced using the nized as safe (GRAS) material. tion equipment used for GSCs at similar encapsulation speeds. same type of mixing VSC materials The most common gelatin substitutes VSC market and encapsulation used to make VSCs are carrageenan, VSC products are currently avail- modified starch, and alginate. Table 1 able in the nutraceutical and cosmetics equipment used for summarizes the market and sourcing markets as well as a few products in the challenges for each. pharmaceutical market. VSCs currently gelatin soft capsules Carrageenan. The global market for constitute approximately 10 to 15 per- carrageenan is approximately 60 to 70 cent of the nutraceutical soft capsule at similar kilotonnes per year, with an expected market in terms of revenue, and their use compound annual growth rate (CAGR) is expected to continue to grow due to encapsulation speeds. of 4 to 5 percent [2]. Seventy to 80 per- increasingly competitive material prices cent of the carrageenan produced is used and optimized processing technology as in the food industry for its gelling, thick- well as expansion into the pharmaceutical market. ening, and stabilizing properties, while less than 10 per- The primary market for VSCs includes consumers cent is used by the pharmaceutical industry. Pharma- who demand vegetarian capsules for cultural, religious, ceutical applications, particularly soft capsules, are still or dietary reasons. VSCs can also be beneficial in appli- considered a niche segment. cations where gelatin is not a suitable capsule polymer, As previously mentioned, the first VSCs consisted of such as high-temperature encapsulation or products with a blend of carrageenan and modified starch (hydroxy- high-pH fill content. Manufacturers are also looking to propyl starch), and most nutraceutical VSCs currently VSC polymers as alternatives to gelatin because gelatin is on the market still use carrageenan-based formulations. Table 1 Market and supply for gelatin substitutes in soft capsules Material Global market Pharma Major pharma Average Raw material Sourcing challenges in 2018 usage suppliers* price (kilotonnes) ($/kg) Carrageenan 60-70 7% ~10-12 11-12 Red seaweeds Limited supply base and concerns VSC: 500 with feedstock availability may tonnes occur due to climate change. Currently, no supply issue with feedstock in major producing regions. Alginates 40 8-10% ~2-4 12-14 Brown seaweed Highly consolidated supply base and GSC: high entry cost for new suppliers. unknown Modified 9,000-10,000 10-15% ~5 4-5 Many, Important to track price movement. starch (as an including: excipient) potato, corn, cassava, etc. Source: Beroe analysis * Indicates the number of suppliers who cater to pharmaceutical applications but not necessarily soft capsule shells. Copyright CSC Publishing Manufacturers offering carrageenan-based soft capsules starch, which is derived from the cassava root [9]. Some include Procaps, Ayanda, Captek, Catalent, and Euro- other modified starches such as corn or pea are used in caps, among others. combination with carrageenan for softgel applications. Carrageenan is produced by two species of cultured Alginate. Europe and North America combined red seaweeds, kappaphycus alvarezii and eucheuma spp. account for more than 50 percent of the global algi- Production of these seaweeds was estimated at 8.3 mil- nate market, which is growing at a CAGR of 1 to 3 per- lion tonnes in 2012 [3]. Indonesia and the Philippines are cent. Growth in the Asia-Pacific market is higher due to the two major producers of this raw material, which sells increased demand from the processed food and pharma- for approximately $1,800 per tonne [4, 5]. ceutical industries. The pharmaceutical market for alginate Climate change has the potential to affect the red is consolidated, with two major suppliers that account for seaweed supply, but suppliers are taking actions to avoid more than 90 percent of global market share [10]. shortages. For example, the carrageenan manufacturer Currently, the pharmaceutical alginate supply is suf- Cargill has committed to sourcing 60 percent of its red ficient to meet demand, and if demand should increase seaweed sustainably by 2025 [6]. suddenly, suppliers focused on food-grade alginate could The number of suppliers manufacturing pharmaceu- switch production to pharmaceutical applications, since tical-grade carrageenan suitable for soft capsule pro- all regulatory approvals are in place. duction is limited, but it is expected that suppliers will The sole raw material for commercial-scale alginate expand and improve their product portfolios due to production is brown seaweed, which is time consuming increased demand from end-user industries. and labor intensive to cultivate. Currently, the brown Modified starch. Global production of modified starch seaweed supply is sufficient to meet the needs of the was between 9 and 10 million tonnes in 2017 [7], and the alginate market, and there are no issues with feedstock import price in the European Union was $922 per tonne, capacity. However, because there is a limited number of which represented a 3.9 percent increase over the previ- qualified suppliers and a high entry cost for new suppli- ous year [8]. Starch has good film-forming properties and ers, alginate buyers do not have much negotiating power good raw material availability, which, along with its price with suppliers. advantage, makes starch-based soft capsules a good low- price alternative to GSCs or other vegetarian capsules. The world’s first carrageenan-free vegan softgel, Plantgels, replaces carrageenan with modified tapioca Table 2 Comparison of processing steps for GSCs versus VSCs Gelatin Modified starch Carrageenan-modified starch Film casting Fast, easy Slow, difficult, sensitive to RH Slow, easy (equipment adaptations) Polymer mass preparation No No Yes specific mixing system High temperature heated- No Yes Yes (extrusion systems can be required) transfer system Conventional die rolls Yes No No Encapsulation Yes spreader boxes design No No (some cases) Required curing step No No Yes Tray-drying (days) + ++ + (oil-based capsules) Batch-to-batch variability No Yes No Process cost $ $$ $$$ Source: Procaps Copyright CSC Publishing VSC manufacturing Table 2 shows a summary of the technical require- As previously mentioned, the properties of the mate- ments for VSC production and compares the pros and rials used to make VSCs differ from gelatin. Raw mate- cons of VSCs and GSCs. Items in red show processing rial and equipment suppliers have had to learn how to differences compared to GSCs due to new equipment, work with these new materials to overcome challenges adaptations to existing equipment, or additional process- related to the resulting capsules’ physical, chemical, and ing steps, such as heated transfer and curing. functional properties, such as soft- In recent years, modified starch ness, leaking, poor dissolution, and suppliers have offered materials with increased dissolution times at stability Raw material and viscosities of about 40,000 to 50,000 conditions. Also, VSC polymers have centipoise that can be handled in higher viscosities and require higher equipment suppliers GSC equipment. In contrast, C-MS processing temperatures than gelatin, formulations can have viscosities as which makes mixing, deaeration, and have had to learn how high as 90,000 centipoise. As the transfer processes challenging. table shows, such polymer masses With
Load more

Recommended publications
  • Characteristic of Gelatine, Carrageenan and Sodium Alginate Hydrosols Treated by Direct Electric Current
    polymers Article Characteristic of Gelatine, Carrageenan and Sodium Alginate Hydrosols Treated by Direct Electric Current Zaneta˙ Król 1,*, Magdalena Malik 2, Krzysztof Marycz 3 and Andrzej Jarmoluk 1 1 Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37/41, 51-630 Wroclaw, Poland; [email protected] 2 Faculty of Chemistry, Wroclaw University of Technology, Smoluchowskiego 23, 50-370 Wroclaw, Poland; [email protected] 3 Department of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, Chelmonskiego 38 C, 50-630 Wroclaw, Poland; [email protected] * Correspondence: [email protected]; Tel.: +48-71-320-7719 Academic Editor: Patrick Ilg Received: 15 July 2016; Accepted: 26 July 2016; Published: 30 July 2016 Abstract: The aim of the study was to investigate the effect of using direct electric current (DC) of 400 mA for five minutes on the physiochemical properties of gelatine (2%, 4%, and 8%), carrageenan (1.5%, 2%, and 2.5%) and sodium alginate (0.75%, 1%, and 1.25%) hydrosols with different sodium chloride concentration. The pH, oxidation-reduction potential (ORP), electrical conductivity (EC), available chlorine concentration (ACC) and rheological parameters were measured. Moreover, Fourier transform infrared spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM) analysis were carried out. The results have shown that pH, ORP, EC and ACC values are changed upon applying DC and the magnitude of change depends on the concentration of the polymer and the addition of sodium chloride. After seven days of storage, the ACC of the samples exposed to DC decreased by 88%–96%.
    [Show full text]
  • Green Algae Strategy.Pdf
    Green Algae Strategy End Oil Imports and Engineer Sustainable Food and Fuel Mark Edwards greenindependence.org ii Key words: algae, cyanobacteria, innovation, nanotechnology, biotechnology, sustainability, green solar energy, food, hunger, eflation, biofuels, pollution, ethanol, jet fuel, agriculture, medicines, pharmaceuticals, health, nutraceuticals, vaccines, reforestation, aquaculture, smoke death, environment, chemical engineering, business, social entrepreneur, social networks and collaboratory. ISBN 1440421846 EAN-13 is 9781440421846 Tempe, Arizona Copyright © 2008, Mark R. Edwards, Rev. 6.6 Green Algae Strategy materials may be copied and used for educational purposes. Cover photo – sushi and algae oil © 2008, Mark R. Edwards iii Dedication To my wonderful life partner Ann Ewen and her passion for great food and loving support and to Sarah Edwards who finishes grace before family meals with “Please God, bless this food and help people who don’t have food get some.” To Jacques Cousteau, who introduced and mentored my introduction to algae and global stewardship through his contributions to the U.S. Naval Academy where he helped initiate a program in oceanography. iv Contents Green Algae Strategy ............................................................................. ii Preface ................................................................................................... ii Biowar І Series Strategy ........................................................................ iii Biowar I ................................................................................................
    [Show full text]
  • Evaluation of Certain Food Additives
    WHO Technical Report Series 990 Evaluation of certain food additives Seventy-ninth report of the Joint FAO/WHO Expert Committee on Food Additives The World Health Organization was established in 1948 as a specialized agency of the United Nations serving as the directing and coordinating authority for international health matters and public health. One of WHO’s constitutional functions is to provide objective and reliable information and advice in the field of human health, a responsibility that it fulfils in part through its extensive programme of publications. The Organization seeks through its publications to support national health strategies and address the most pressing public health concerns of populations around the world. To respond to the needs of Member States at all levels of development, WHO publishes practical manuals, handbooks and training material for specific categories of health workers; internationally applicable guidelines and standards; reviews and analyses of health policies, programmes and research; and state-of-the- art consensus reports that offer technical advice and recommendations for decision-makers. These books are closely tied to the Organization’s priority activities, encompassing disease prevention and control, the development of equitable health systems based on primary health care, and health promotion for individuals and communities. Progress towards better health for all also demands the global dissemination and exchange of information that draws on the knowledge and experience of all WHO’s Member countries and the collaboration of world leaders in public health and the biomedical sciences. To ensure the widest possible availability of authoritative information and guidance on health matters, WHO secures the broad international distribution of its publications and encourages their translation and adaptation.
    [Show full text]
  • Social and Economic Dimensions of Seaweed Farming: a Global Review
    IIFET 2012 Tanzania Proceedings SOCIAL AND ECONOMIC DIMENSIONS OF SEAWEED FARMING: A GLOBAL REVIEW Diego Valderrama, Food and Resource Economics Department, University of Florida, USA, [email protected] ABSTRACT Seaweed farming based primarily on the culture of Kappaphycus and Eucheuma species has grown significantly in the Philippines and Indonesia over the last two decades, with growth also taking place at a smaller scale in Tanzania, India and a few other developing countries. Unlike other forms of aquaculture, seaweed farming foregoes the use of feed and fertilizers and has minimum technological and capital requirements. In addition, growout cycles are short, normally lasting less than two months. Given these unique characteristics, seaweed farming has generated substantial socio-economic benefits to marginalized coastal communities in developing countries, most of which have reduced access to alternative economic activities. In some communities, seaweed farming has emerged as the most relevant livelihood strategy. This paper summarizes the findings of a recent FAO review on the social and economic dimensions of seaweed farming in six countries in Asia (the Philippines, Indonesia, India), Africa (Tanzania), Oceania (Solomon Islands), and Latin America (Mexico). Each case study documented the evolution of the farming sector and examined the mix of public sector policies and private sector involvement leading to growth of the activity. Given the rising global demand for seaweed-derived products, seaweed farming has the potential to generate further socio-economic benefits to coastal communities in tropical regions; however, a number of challenges and constraints (some of which are country-specific) will need to be addressed to fully take advantage of these opportunities.
    [Show full text]
  • Carrageenans in Meat Systems Zoraida Defreitas Iowa State University
    Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1994 Carrageenans in meat systems Zoraida DeFreitas Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Agriculture Commons, and the Food Science Commons Recommended Citation DeFreitas, Zoraida, "Carrageenans in meat systems " (1994). Retrospective Theses and Dissertations. 10551. https://lib.dr.iastate.edu/rtd/10551 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in ^ewriter face, while others may be from aity type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps.
    [Show full text]
  • Extraction of Kappa Carrageenan from Local Seaweed
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by UMP Institutional Repository EXTRACTION OF KAPPA CARRAGEENAN FROM LOCAL SEAWEED ADZLIN BIN HUSIN Thesis submitted to the Faculty of Chemical and Natural Resource Engineering in fulfilment of the requirements for the award of the Degree of Bachelor of Chemical Engineering Faculty of Chemical and Natural Resources Engineering UNIVERSITI MALAYSIA PAHANG JANUARY 2014 EXTRACTION OF HALAL KAPPA CARRAGEENAN FROM LOCAL SEAWEED ABSTRACT There are three main types of seaweed, which is red, brown and green seaweed. The most famous is red seaweed, which contains carrageenan. Carrageenan is a generic name of viscosifying and gel forming polysaccharides family. Kappa carrageenan is predominantly produced from Eucheuma cottonii (red seaweed) (Van De Velde et al, 2002) that normally found in the ocean of Philippines, Malaysia and Indonesia. It is widely use since it has excellent physical functional properties such as gelling, thickening and stabilizing abilities (Hoffman et al, 1995). Today, most of Muslim take medicine with gelatin capsules. Gelatin is a mixture of proteins and peptides produced by partial hydolysis of collagen which is extracted from the skin, boiled crushed horn, hoof and bones, connective tissues, organs and some intestines of animals such as domesticated cattle and pigs. An alternative for Muslims to obtain medicine from halal capsules source, the kappa carrageenan from local seaweed is extracted to replace the uses of gelatin in production of medicine capsules (Campo et al, 2009). Kappa carrageenan has a good gel strength which is similar to animal gelatin characteristic. The alkaline treatment is used to modify and promote gel formation in extraction of carrageenan.
    [Show full text]
  • Carrageenan Modulates the Mode of Action of Κ-Carrageenase from Pseudoalteromonas Carrageenovora Maud Lemoine, Pi Nyvall Collén, William Helbert
    Physical state of κ-carrageenan modulates the mode of action of κ-carrageenase from Pseudoalteromonas carrageenovora Maud Lemoine, Pi Nyvall Collén, William Helbert To cite this version: Maud Lemoine, Pi Nyvall Collén, William Helbert. Physical state of κ-carrageenan modulates the mode of action of κ-carrageenase from Pseudoalteromonas carrageenovora. Biochemical Journal, Port- land Press, 2009, 419 (3), pp.545-553. 10.1042/BJ20080619. hal-00479000 HAL Id: hal-00479000 https://hal.archives-ouvertes.fr/hal-00479000 Submitted on 30 Apr 2010 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Biochemical Journal Immediate Publication. Published on 05 Feb 2009 as manuscript BJ20080619 1 Physical state of N-carrageenan modulates the mode of action of N- 2 carrageenase from Pseudoalteromonas carrageenovora 3 4 Maud Lemoine, Pi Nyvall Collén and William Helbert* 5 6 Université Pierre et Marie Curie, Paris VI, CNRS, Marine Plants and Biomolecules, UMR 7 7139, Station Biologique, BP 74, F29680 Roscoff Cedex, France 8 9 10 *Corresponding author; Fax: + 33 298 292 332, Email: [email protected] 11 12 13 Short/running title: Processivity of N-carrageenase from Pseudoalteromonas carrageenovora 14 Keywords: Carrageenan, N-carrageenase, processivity, gel, mode of action THIS IS NOT THE VERSION OF RECORD - see doi:10.1042/BJ20080619 Accepted Manuscript 1 Licenced copy.
    [Show full text]
  • 11-28-2016 Carrageenan
    Week of November 7, 2016 – Carrageenans Recently, I went to the refrigerator in our break room to look for some “half n’ half” to add to my morning cup of Joe. Upon opening the door I noticed two containers of almond milk; both with bold letters stating, “CARRAGEENAN FREE.” Carrageenan free? OK, so what’s that? I thought it might be something like gluten; that portion of grains, such as wheat, barley and oats that have recently received some serious criticism due to causing allergic reactions and dysfunction of the immune system (aka celiac disease). I’m sure we have all been to restaurants and health food stores where there are signs that boldly tout gluten-free products. While gluten and carrageenan are “natural” ingredients, they are, by no stretch of the imagination, similar. Carrageenans are a family of linear sulphated polysaccharides (long- chained sugar molecules) that are extracted from red edible seaweeds. They are large, highly flexible molecules that curl, forming helical structures. This gives them the ability to form a variety of different gels at room temperature. As such, they are widely used in the food industry for their gelling, thickening, and stabilizing properties. Their main application is in dairy and meat products as they create strong binds to food proteins. Carrageenans were introduced on an industrial scale in the 1930s and, while used in in foods, have no nutritional value. Typical products that use carrageenans as either a thickener or emulsifier to improve food texture include ice cream (dairy and non-dairy), yogurt, cottage cheese, soy milk and processed foods.
    [Show full text]
  • Carrageenan Origin
    Things to Know About 5 Carrageenan Non-GMO* Halal Organic* Kosher ORIGIN 1 Sustainability Fact Carrageenan is extracted Food and beverage from red seaweed, most manufacturers can use commonly of the carrageenan to stabilize Chondrus crispus, foods at room Eucheuma cottonii, temperature, reducing Eucheuma spinosum, or food waste and transportation and Gigartina stellata species. storage costs. Carrageenan... 2 PROCESSING 1. Seaweed is harvested from the ocean. ... is available in three grades: iota, kappa, and lambda. 2. The seaweed is washed in a hot ... can be harvested from the water and alkaline coasts of Northern Europe, broth, then rinsed. Southeast Asia and East Africa. 3. The seaweed is dried and chopped ... can extend the shelf into small pieces. life of frozen finished goods by improving 3. The dried pieces freeze-thaw stability. are milled into powder for use. 3 FUNCTIONALITY DID YOU KNOW? Improves Controls Carrageenan Mouthfeel Syneresis originated in Ireland and can be traced back almost 600 years. Many Binds Provides communities Water Set around the world keep this ingredient stocked in their home Provides Prevents Suspension Whey-Off kitchens. REGULATORY 4 SQF III Facility Regulatory Experts Foreign Supplier Verification Program Iota Carrageenan APPLICATIONS 5 Carrageenan can be used in a Looking to add Carrageenan range of applications. to your next formulation? Try our ® Ticaloid Ice Cream 710 H-96 Powder. Yogurt Reach out to a Gum Dairy-Alternatives Guru for a sample! Dessert Gels Flavored Milks Texture & Stability Challenges SOLV® ED Call the Gum Gurus : (800) 899-3953 Chat with the Gum Gurus: ticgums.com/ch at * Option Available .
    [Show full text]
  • Functionality of Ingredients and Additives in Plant-Based Meat Analogues
    foods Review Functionality of Ingredients and Additives in Plant-Based Meat Analogues Konstantina Kyriakopoulou * , Julia K. Keppler and Atze Jan van der Goot Food Process Engineering, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands; [email protected] (J.K.K.); [email protected] (A.J.v.d.G.) * Correspondence: [email protected] Abstract: Meat analogue research and development focuses on the production of sustainable products that recreate conventional meat in its physical sensations (texture, appearance, taste, etc.) and nutritional aspects. Minced products, like burger patties and nuggets, muscle-type products, like chicken or steak-like cuts, and emulsion products, like Frankfurter and Mortadella type sausages, are the major categories of meat analogues. In this review, we discuss key ingredients for the production of these novel products, with special focus on protein sources, and underline the importance of ingredient functionality. Our observation is that structuring processes are optimized based on ingredients that were not originally designed for meat analogues applications. Therefore, mixing and blending different plant materials to obtain superior functionality is for now the common practice. We observed though that an alternative approach towards the use of ingredients such as flours, is gaining more interest. The emphasis, in this case, is on functionality towards use in meat analogues, rather than classical functionality such as purity and solubility. Another trend is the exploration of novel protein sources such as seaweed, algae and proteins produced via fermentation (cellular agriculture). Citation: Kyriakopoulou, K.; Keywords: plant protein; meat analogues; vegetarian sausage; vegetarian burger; vegetarian steak; Keppler, J.K.; van der Goot, A.J.
    [Show full text]
  • Carrageenan Oligosaccharides and Their Degrading Bacteria Induce Intestinal Infammation in Germ-Free Mouse
    Carrageenan oligosaccharides and their degrading bacteria induce intestinal inammation in germ-free mouse Yeshi Yin ( [email protected] ) Zhejiang Academy of Agricultural Sciences https://orcid.org/0000-0001-6866-5525 Miaomiao Li Ocean University of China Weizhong Gu Zhejiang University Benhua Zeng Third Military Medical University Wei Liu Zhejiang Academy of Agriculture of Science Liying Zhu Zhejiang Academy of Agriculture of Sciences Xionge Pi Zhejiang Academy of Agriculture of Sciences Donald A Primerano Marshall University Hongwei D Yu Marshall University Hong Wei Third Military Medical University Guangli Yu Ocean University of China Xin Wang Zhejiang Academy of Agriculture of Sciences Research Keywords: carrageenans, carrageenan oligosaccharides, oligosaccharide degrading bacteria, intestinal inammatory, germ-free mouse Posted Date: May 8th, 2020 Page 1/24 DOI: https://doi.org/10.21203/rs.3.rs-26790/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 2/24 Abstract Background: Carrageenans (CGNs) are widely used in food and pharmaceutical industries. However, the safety of CGNs is still under debate, because degraded CGNs have been reported to promote an intestinal inammatory response in animal models. Here, we studied the relationship among CGNs, human gut microbiota, and the host inammatory response. Methods: TLC was selected for detecting the degradation of KCPs by human gut microbiota in vitro batch fermentation system. PCR-DGGE and real time PCR were used for studying bacterial community. ESI-MS was used for KCPs structure analysis. Hematoxylin-eosin staining (HE), immunohistochemistry (IHC) and RNA-seq were used to evaluated the KCPs on host inammation response in germ-free mice.
    [Show full text]
  • Gelation and Melting of a Mixed Carrageenan-Gelatin Gel
    J. Home Econ. J pn. Vol. 44 No. 12 999-1005 (1993) Gelation and Melting of a Mixed Carrageenan-Gelatin Gel Reiko SHIMADA, Keiko KUMENO,* Hiro AKABANE * * and Nobuko NAKAHAMA * Junior College at Mishima, Nihon University, Mishima, Shizuoka 411, Japan * Japan Women's University, Bunkyo-ku, Tokyo 112, Japan * * Kantogakuin Women's Junior College, Kanazawa-ku, Yokohama 236, Japan The rheological changes in a mixture of two different gelling agents were studied. A 0.9% (w/v) ƒÈ-carrageenan solution (C(1)), a 4% (w/v) gelatin solution (G(1)) and mixed samples in three proportions were prepared. The gelling temperature and melting temperature were mea- sured. Changes in the dynamic viscoelasticity when the state of the gel changed were determined with a Rheolograph-Sol instrument, and the endothermic reaction during melting was also examined by DSC. The gelling and melting temperatures for C(1) were the highest among all the samples. These values for the mixed samples were reduced according to the decreasing ratio of carrageenan. Gelling and melting of the samples were observed when the storage modulus was in the range of 6-8 N/m2. This suggests that the change point for state exists in this range. The DSC result revealed that the peak heights of the mixed gelatin-carrageenan samples were lower and their shapes were broader than those of the pure samples. The peak temperature of the endothermic reaction and the melting temperature corresponded well. (Received February 2, 1993) Keywords: carrageenan/gelatin mixed gel, gelation, melting, DSC, dynamic viscoelasticity. bean gum, in different ratios, and have also reported INTRODUCTION rheological changes during gelling of pure samples.4) Both gelatin and carrageenan are widely used However, there is no information available about as gelling agents for cookery and food applications global changes in the gelling properties, including since both form a highly transparent gel.
    [Show full text]