QPCT (NM 012413) Human Recombinant Protein Product Data

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QPCT (NM 012413) Human Recombinant Protein Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TP308388 QPCT (NM_012413) Human Recombinant Protein Product data: Product Type: Recombinant Proteins Description: Recombinant protein of human glutaminyl-peptide cyclotransferase (QPCT) Species: Human Expression Host: HEK293T Tag: C-Myc/DDK Predicted MW: 37.8 kDa Concentration: >50 ug/mL as determined by microplate BCA method Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Storage: Store at -80°C. Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. RefSeq: NP_036545 Locus ID: 25797 UniProt ID: Q16769 RefSeq Size: 1719 Cytogenetics: 2p22.2 RefSeq ORF: 1083 Synonyms: GCT; QC; sQC Summary: This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. [provided by RefSeq, Jul 2008] Protein Families: Protease This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 QPCT (NM_012413) Human Recombinant Protein – TP308388 Product images: Coomassie blue staining of purified QPCT protein (Cat# TP308388). The protein was produced from HEK293T cells transfected with QPCT cDNA clone (Cat# [RC208388]) using MegaTran 2.0 (Cat# [TT210002]). This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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QPCT (4E11): Sc-517122
SANTA CRUZ BIOTECHNOLOGY, INC. QPCT (4E11): sc-517122 BACKGROUND APPLICATIONS QPCT (glutaminyl-peptide cyclotransferase) is a 361 amino acid protein that QPCT (4E11) is recommended for detection of QPCT of human origin by belongs to the glutaminyl-peptide cyclotransferase family. QPCT is responsible Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), for the presence of pyroglutamyl residues in many neuroendocrine peptides. immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell QPCT binds one zinc ion per subunit and has a bias against acidic and trypto- lysate)] and solid phase ELISA (starting dilution 1:30, dilution range 1:30- phan residues adjacent to the N-terminal glutaminyl residue. The human QPCT 1:3000). gene shares 86% overall sequence identity with its bovine homolog. QPCT Suitable for use as control antibody for QPCT siRNA (h): sc-94268, QPCT contains an N-terminal signal peptide region, several glycosylation and phos- shRNA Plasmid (h): sc-94268-SH and QPCT shRNA (h) Lentiviral Particles: phorylation sites and two cysteine residues conserved between the bovine sc-94268-V. and human enzymes. Existing as two alternatively spliced isoforms, the QPCT gene is conserved in chimpanzee, canine, bovine, mouse, rat, chicken, fruit fly, Molecular Weight of QPCT isoforms: 41/35 kDa. mosquito, M. grisea and N. crassa, and maps to human chromosome 2p22.2. RECOMMENDED SUPPORT REAGENTS REFERENCES To ensure optimal results, the following support reagents are recommended: 1. Busby, W.H., et al. 1987. An enzyme(s) that converts glutaminyl-peptides 1) Western Blotting: use m-IgGk BP-HRP: sc-516102 or m-IgGk BP-HRP (Cruz into pyroglutamyl-peptides.
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