DOCSLIB.ORG

(Acrylamide) Hydrogels by Tridax Procumbens Leaf Extract and Their Antibacterial Activity

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(Acrylamide) Hydrogels by Tridax Procumbens Leaf Extract and Their Antibacterial Activity Hindawi Publishing Corporation International Journal of Carbohydrate Chemistry Volume 2013, Article ID 539636, 10 pages http://dx.doi.org/10.1155/2013/539636 Research Article A Green Approach to Synthesize Silver Nanoparticles in Starch-co-Poly(acrylamide) Hydrogels by Tridax procumbens Leaf Extract and Their Antibacterial Activity Siraj Shaik,1 Madhusudana Rao Kummara,2 Sudhakar Poluru,1 Chandrababu Allu,1 Jaffer Mohiddin Gooty,3 Chowdoji Rao Kashayi,4 and Marata Chinna Subbarao Subha1 1 Department of Chemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh 515 003, India 2 DepartmentofPolymerScienceandEngineering,PusanNationalUniversity,Busan609-735,RepublicofKorea 3 DepartmentofMicrobiology,SriKrishnadevarayaUniversity,Anantapur,AndhraPradesh515003,India 4 Department of Polymer Science & Tech, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh 515 003, India Correspondence should be addressed to Marata Chinna Subbarao Subha; [email protected] Received 31 August 2013; Accepted 13 November 2013 Academic Editor: Roland J. Pieters Copyright © 2013 Siraj Shaik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A series of starch-co-poly(acrylamide) (starch-co-PAAm) hydrogels were synthesized by employing free radical redox poly- + merization. A novel green approach, Tridax procumbens (TD) leaf extract, was used for reduction of silver ions (Ag )into silver nanoparticles in the starch-co-PAAm hydrogel network. The formation of silver nanoparticles was confirmed byUV- visible spectroscopy (UV-Vis), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (X-RD) studies. 22% of weight loss difference between hydrogel and silver nanocomposite hydrogel (SNCH) clearly indicates the formation of silver nanoparticles by TGA. TEM images indicate the successful incorporation of silver nanoparticles ranging from 5 to 10 nm in size and spherical in shape with a narrow size distribution. These developed SNCHs were used to study the antibacterial activity by inhibition zone method against gram-positive and gram-negative bacteria such as Bacillus and Escherichia coli. The results indicated that these SNCHs can be used potentially for biomedical applications. 1. Introduction forces that do not prevent the penetration of water through the network [5]. Based on these properties the hydrogels have Hydrogels have three-dimensional polymeric networks that been used recently as templates for production of metallic are fabricated from polymers stabilized through physical or nanoparticles. Hence the design and development of metal chemical crosslinking. They absorb large quantities of water nanoparticles dispersed in polymer matrix have attracted without losing their structural integrity [1]. Since they mimic potential applications in various fields like electrical, optical, body tissues and respond to external stimuli, they are made or mechanical properties [6, 7] making them valuable for important and promising forms of biomaterials for various applications in areas like optics [8], photo imaging and applications including tissue engineering, controlled drug patterning [9], electronic devices [10], sensors and biosensors release devices, biosensors, and mechanical actuators [2– [11–13], catalysis [14, 15], and antibacterial and antimicrobial 4]. Due to the presence of water solubilizing groups, such coatings [16]. Silver nanoparticles (Ag NPs) have attracted as –OH, –COOH, –CONH2,–CONH–,and–SO3H, these considerable interest in biological studies because of their hydrogels show higher hydrophilicity. The three-dimensional ease of preparation, good biocompatibility, and relatively network of hydrogel provides relative stability to its structure. large surface area [17, 18]. Ag NPs have many important appli- Their swollen state results from a balance between the cations in biomedical fields, sensors, and filters [19]. Addi- dispersing forces acting on hydrated chains and cohesive tionally, silver is a potential antibacterial agent [20]andis 2 International Journal of Carbohydrate Chemistry thus used as a sterilizer for removing bacteria from drinking The developed SNCHs may be biodegradable and nontoxic water [21, 22]. For economic and efficient use of silver, silver andwerepotentialmaterialstobeusedforbiomedical nanoparticle composites have been developed and tested for applications. antimicrobial activity. Starch is one of the natural, renewable, and biodegradable 2. Experimental polymers produced by many plants as a source of stored energy. Starch consists of two types of molecules: amylose 2.1. Materials. AR grade soluble starch (potato), silver nitrate (normally 20–30%) and amylopectin (normally 70–80%). (AgNO3), potassium persulfate (KPS), maleic acid (MA), and The relative proportion of amylose to amylopectin depends sodium hydroxide (NaOH) were purchased from SD Fine 1 onthesourceofstarch.Itisversatileandcheapandhas Chemicals,Mumbai,India.Acrylamide(AAm),, -meth- 1 11 many uses as thickener, water binder, emulsion stabilizer, and ylenebisacrylamide (MBA), and ,, , -tetramethyl- gelling agent [23]. Starch granules are insoluble in cold water ethylenediamine (TEMED) were purchased from Aldrich but imbibe water reversibly and swell slightly [24]. Maleic Chemical Company Inc. (Milwaukee, WI, USA). All chem- acid (MA) is a nontoxic and biodegradable hydrophilic icals were used without further purification. Double-distilled substance which is used as a type of food additive in (DD) water is used throughout the experiment. processing industry. The modification of starch with MA increases its hydrophilicity by converting it into ester form by the reaction between starch and MA to form starch macro- 2.2. Preparation of Tridax procumbens (TD) Leaf Extract. monomer [25]. The chemical modification of starch with MA Leaf extracts were prepared by a green process technique, and tartaric acid improves its properties without sacrificing using the standard procedure described by Prasad et al. [39]. its biodegradability and biocompatibility [25–27]. Several Tridax procumbens leaves were collected from Tridax pro- reports are present on acrylamide (AAm) hydrogels that were cumbens plants at our university campus in Sri Krishnade- developed because of their hydrophilic and inert nature that varaya University (Anantapur, Andhra Pradesh, India), thor- makes them suitable for applications in medicine and phar- oughly washed with DD water to remove the dust particles, macy [28, 29].Polyacrylamidehasbeenusedincontactlenses and then dried in sun to remove the residual moisture. From for a long time, and it has well been evaluated as a sustained- this 20 g leaves were chopped finely and boiled in a 250 mL release wound-dressing material [30]. glass beaker along with 200 mL of sterile DD water for 10 min In continuation of our work [31]onusageofpoly- in order to extract the contents of the leaves. After boiling the mers in biomedical applications, the present research paper colour, the aqueous solution changed from watery to yellow explores a simple and facile method to synthesize starch- colour. The aqueous extract was separated by filtration with co-poly(acrylamide) (starch-co-PAAm) hydrogels and silver 2.5 m Whatman filter paper (Sigma-Aldrich, USA) and then nanocomposite hydrogels (SNCHs) by using a green process stored at room temperature. + in which the Ag ions are successfully reduced by Tridax procumbens (TD) leaf extract. Starch, MA, and AAm were 2.3. Synthesis of Starch-co-PAAm Hydrogels and Their Sil- chosen on the basis of their significant characteristics in ver Nanocomposite Hydrogels (SNCHs). Gelatinized starch biomedical fields. The most popular way used for the direct solution was prepared by mixing a quantitative amount of synthesis of metal ions into nanoparticles is through chemical starch powder in 10.0 mL of DD water and 1.0 mL of 0.5 M reductionmethodbyusingseveralreagentssuchassodium sodium hydroxide (NaOH) solution, and the mixture was ∘ borohydride, sodium hypophosphite, and sodium citrate. heated in a water bath at 90 Cfor10minwithcontinuous Several reports are presented for the development of Ag NPs magnetic stirring. A predetermined amount of MA was then by using such reducing agents [32, 33]. But these reducing added to the gelatinized starch solution and the resulting agents may have environmental problems due to their tox- ∘ mixturewasfurtherheatedinawaterbathat80Cfor4h icity, so a considerable interest to use nontoxic materials for [25]. Subsequently, a quantitative amount of acrylamide was reduction of Ag NPs is growing rapidly these days. In order to ∘ added and stirred for half an hour at 50 C, after that cross- avoid such type of toxic reagents, recently plant extracts have beenusedforproductionofAgNPsduetotheircost-effective linker (MBA) and initiator (KPS) were added according to the nature and also ambient conditions for reduction [34–37]. formulations mentioned in the Table 1.Finally,anaqueous Therefore, the development of metal nanoparticles based on solution of TEMED was added to the solution and the same natural extracts is considered the most appropriate method. temperature was maintained for another 10 min. After Recently Tridax procumbens (TD) leaf extract was used as completion of free radical polymerization, the synthesized reducing agent for preparation
Load more

Recommended publications
  • Self-Healing Polymer Nanocomposite Materials: a Review
    Elsevier Editorial System(tm) for Polymer Manuscript Draft Manuscript Number: POLYMER-15-9R2 Title: Self-Healing Polymer Nanocomposite Materials: A Review Article Type: SI: Self-Healing Polymers (Invited) Section/Category: Physical Chemistry of Polymers Keywords: Polymer; Self Healing; Nano composites; Properties Corresponding Author: Prof. Michael R. Kessler, Ph.D. Corresponding Author's Institution: Washington State University First Author: Vijay Kumar Thakur, Ph.D. Order of Authors: Vijay Kumar Thakur, Ph.D.; Michael R. Kessler, Ph.D. Manuscript Region of Origin: USA Abstract: During the last few years, different kinds of autonomic and non-autonomic self-healing materials have been prepared using diverse techniques for a number of applications. The incorporation of suitable functionalities into these materials facilitates a healing mechanism that is triggered by damage/ rupture as well as various chemistries. This article presents a detailed study of the self-healing properties of different kinds of polymer nanocomposites utilizing a number of healing mechanisms, including the addition of several healing agents. The article will also provide an overview of different chemistries employed in the preparation of self-healing polymer nanocomposites, along with their advantages and disadvantages. *Graphical Abstract (for review) *Manuscript Click here to view linked References Self-Healing Polymer Nanocomposite Materials: A Review Vijay Kumar Thakur and Michael R. Kessler* School of Mechanical and Materials Engineering, Washington State University, WA, USA. Tel.: +1 509 335 8654; Fax +1 509 335 4662 E-mail address: [email protected]; [email protected] Abstract During the last few years, different kinds of autonomic and non-autonomic self-healing materials have been prepared using diverse techniques for a number of applications.
    [Show full text]
  • Two-Dimensional Nanomaterials
    Science Bulletin 64 (2019) 1707–1727 Contents lists available at ScienceDirect Science Bulletin journal homepage: www.elsevier.com/locate/scib Review Two-dimensional nanomaterials: fascinating materials in biomedical field ⇑ Tingting Hu a, Xuan Mei a, Yingjie Wang b, Xisheng Weng b, , Ruizheng Liang a,*, Min Wei a,* a State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China b Department of Orthopaedics, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China article info abstract Article history: Due to their high anisotropy and chemical functions, two-dimensional (2D) nanomaterials have attracted Received 23 July 2019 increasing interest and attention from various scientific fields, including functional electronics, catalysis, Received in revised form 22 August 2019 supercapacitors, batteries and energy materials. In the biomedical field, 2D nanomaterials have made sig- Accepted 12 September 2019 nificant contributions to the field of nanomedicine, especially in drug/gene delivery systems, multimodal Available online 20 September 2019 imaging, biosensing, antimicrobial agents and tissue engineering. 2D nanomaterials such as graphene/ graphene oxide (GO)/reduced graphene oxide (rGO), silicate clays, layered double hydroxides (LDHs), Keywords: transition metal dichalcogenides (TMDs), transition metal oxides (TMOs), black phosphorus (BP), graphi- 2D nanomaterials tic carbon nitride (g-C N ), hexagonal boron nitride (h-BN), antimonene (AM), boron nanosheets (B NSs) Drug delivery 3 4 Tissue engineering and tin telluride nanosheets (SnTe NSs) possess excellent physical, chemical, optical and biological prop- Biosensing erties due to their uniform shapes, high surface-to-volume ratios and surface charge. In this review, we first introduce the properties, structures and synthetic strategies of different configurations of 2D nano- materials.
    [Show full text]
  • A Review on Cellulose Nanocrystals As Promising Biocompounds for the Synthesis of Nanocomposite Hydrogels
    Accepted Manuscript Title: A review on cellulose nanocrystals as promising biocompounds for the synthesis of nanocomposite hydrogels Authors: Jamileh Shojaeiarani, Dilpreet Bajwa, Alimohammad Shirzadifar PII: S0144-8617(19)30417-5 DOI: https://doi.org/10.1016/j.carbpol.2019.04.033 Reference: CARP 14807 To appear in: Received date: 17 January 2019 Revised date: 10 March 2019 Accepted date: 7 April 2019 Please cite this article as: Shojaeiarani J, Bajwa D, Shirzadifar A, A review on cellulose nanocrystals as promising biocompounds for the synthesis of nanocomposite hydrogels, Carbohydrate Polymers (2019), https://doi.org/10.1016/j.carbpol.2019.04.033 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A review on cellulose nanocrystals as promising biocompounds for the synthesis of nanocomposite hydrogels Jamileh Shojaeiarania,*, Dilpreet Bajwab, Alimohammad Shirzadifarc *Corresponding Author a Department of Mechanical Engineering, North Dakota State University, Fargo, ND 58102, United States Email: [email protected] , Phone: +1-701-799-7759, ORCID: 0000-0002-1882-0061 b Department of Mechanical Engineering, North Dakota State University, Fargo, ND 58102, United States Email: [email protected] , Phone: +1-701-231-7279 ORCID: 0000-0001-9910-8035 c Department of Agricultural and Biosystems Engineering, North Dakota State University, Fargo, ND, USA Email: [email protected] , Phone: +1-701-799-7780 Highlights ACCEPTED The application of hydrogels containing MANUSCRIPT CNCs are intensively studied.
    [Show full text]
  • Hydrogel Based Sensors for Biomedical Applications: an Updated Review
    polymers Review Hydrogel Based Sensors for Biomedical Applications: An Updated Review Javad Tavakoli 1,* and Youhong Tang 2,* ID 1 Medical Device Research Institute, College of Science and Engineering, Flinders University, Adelaide 5042, SA, Australia 2 Institute for Nano Scale Science & Technology, College of Science and Engineering, Flinders University, Adelaide 5042, SA, Australia * Correspondence: javad.tavakoli@flinders.edu.au (J.T.); youhong.tang@flinders.edu.au (Y.T.) Received: 20 July 2017; Accepted: 12 August 2017; Published: 16 August 2017 Abstract: Biosensors that detect and convert biological reactions to a measurable signal have gained much attention in recent years. Between 1950 and 2017, more than 150,000 papers have been published addressing the applications of biosensors in different industries, but to the best of our knowledge and through careful screening, critical reviews that describe hydrogel based biosensors for biomedical applications are rare. This review discusses the biomedical application of hydrogel based biosensors, based on a search performed through Web of Science Core, PubMed (NLM), and Science Direct online databases for the years 2000–2017. In this review, we consider bioreceptors to be immobilized on hydrogel based biosensors, their advantages and disadvantages, and immobilization techniques. We identify the hydrogels that are most favored for this type of biosensor, as well as the predominant transduction strategies. We explain biomedical applications of hydrogel based biosensors including cell metabolite and pathogen detection, tissue engineering, wound healing, and cancer monitoring, and strategies for small biomolecules such as glucose, lactate, urea, and cholesterol detection are identified. Keywords: hydrogel based biosensor; hydrogel; bioreceptors; immobilization; biomedical application; transduction strategies 1.
    [Show full text]
  • Nanocomposite Hydrogels: Advances in Nanofillers Used for Nanomedicine
    gels Review Nanocomposite Hydrogels: Advances in Nanofillers Used for Nanomedicine Arti Vashist 1,*, Ajeet Kaushik 1 , Anujit Ghosal 2, Jyoti Bala 1, Roozbeh Nikkhah-Moshaie 1, Waseem A. Wani 3, Pandiaraj Manickam 4 and Madhavan Nair 1,* 1 Department of Immunology & Nano-Medicine, Institute of NeuroImmune Pharmacology, Centre for Personalized Nanomedicine, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199, USA; akaushik@fiu.edu (A.K.); jbala@fiu.edu (J.B.); rnikkhah@fiu.edu (R.N.-M.) 2 School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India; [email protected] 3 Department of Chemistry, Govt. Degree College Tral, Kashmir, J&K 192123, India; [email protected] 4 Electrodics and Electrocatalysis Division, CSIR-Central Electrochemical Research Institute, Karaikudi 630006, Tamil Nadu, India; [email protected] * Correspondence: avashist@fiu.edu (A.V.); nairm@fiu.edu (M.N.) Received: 18 June 2018; Accepted: 23 August 2018; Published: 6 September 2018 Abstract: The ongoing progress in the development of hydrogel technology has led to the emergence of materials with unique features and applications in medicine. The innovations behind the invention of nanocomposite hydrogels include new approaches towards synthesizing and modifying the hydrogels using diverse nanofillers synergistically with conventional polymeric hydrogel matrices. The present review focuses on the unique features of various important nanofillers used to develop nanocomposite hydrogels and the ongoing development of newly hydrogel systems designed using these nanofillers. This article gives an insight in the advancement of nanocomposite hydrogels for nanomedicine. Keywords: biomaterials; nanocomposite hydrogels; nanomedicine; biomedical applications; carbon nanotubes; pH responsive; biosensors 1. Introduction The most emerging field of nanomedicine has come up with diverse biomedical applications ranging from optical devices, biosensors, advanced drug delivery systems, and imaging probes.
    [Show full text]
  • Multifunctional Hydrogel Nanocomposites for Biomedical Applications
    Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 22 February 2021 doi:10.20944/preprints202102.0449.v1 Review Multifunctional Hydrogel Nanocomposites for Biomedical Applications Emma Barrett-Catton†, Murial L. Ross† and Prashanth Asuri* Department of Bioengineering, Santa Clara University, Santa Clara, CA 95053, USA; [email protected] (E.B.-C.); [email protected] (M.L.R.) * Correspondence: [email protected]; Tel.: +1408-551-3005 † These authors contributed equally to this work. Abstract: Hydrogels are used for various biomedical applications due to their biocompatibility, ca- pacity to mimic the extracellular matrix, and ability to encapsulate and deliver cells and therapeu- tics. However, traditional hydrogels have a few shortcomings, especially regarding their physical properties, thereby limiting their broad applicability. Recently, researchers have investigated the incorporation of nanoparticles (NPs) into hydrogels to improve and add to the physical and bio- chemical properties of hydrogels. This brief review focuses on papers that describe the use of nano- particles to improve more than one property of hydrogels. Such multifunctional hydrogel nanocom- posites have enhanced potential for various applications, including tissue engineering, drug deliv- ery, wound healing, bioprinting and biowearable devices. Keywords: multifunctional; hydrogel nanocomposties; tissue engineering; drug delivery; wound healing; bioprinting; biowearable devices 1. Introduction Hydrogels have broad applicability in the biomedical industry due to their unique and tunable physical and biochemical properties [1–3]. However, their poor mechanical strength limits their uses to solely non-load bearing applications [4–9]. Therefore, re- searchers have investigated improving the mechanical properties of hydrogels via multi- ple approaches including, but not limited to, the incorporation of nanoparticles or the ad- dition of a second polymer network to form interpenetrating- or double-network (IPN or DN) hydrogels [2,10–16].
    [Show full text]
  • Multifunctional Hydrogel Nanocomposites for Biomedical Applications
    polymers Review Multifunctional Hydrogel Nanocomposites for Biomedical Applications Emma Barrett-Catton †, Murial L. Ross † and Prashanth Asuri * Department of Bioengineering, Santa Clara University, Santa Clara, CA 95053, USA; [email protected] (E.B.-C.); [email protected] (M.L.R.) * Correspondence: [email protected]; Tel.: +1-408-551-3005 † These authors contributed equally to this work. Abstract: Hydrogels are used for various biomedical applications due to their biocompatibility, capacity to mimic the extracellular matrix, and ability to encapsulate and deliver cells and therapeu- tics. However, traditional hydrogels have a few shortcomings, especially regarding their physical properties, thereby limiting their broad applicability. Recently, researchers have investigated the incorporation of nanoparticles (NPs) into hydrogels to improve and add to the physical and biochem- ical properties of hydrogels. This brief review focuses on papers that describe the use of nanoparticles to improve more than one property of hydrogels. Such multifunctional hydrogel nanocomposites have enhanced potential for various applications including tissue engineering, drug delivery, wound healing, bioprinting, and biowearable devices. Keywords: multifunctional; hydrogel nanocomposties; tissue engineering; drug delivery; wound healing; bioprinting; biowearable devices Citation: Barrett-Catton, E.; Ross, M.L.; Asuri, P. Multifunctional 1. Introduction Hydrogel Nanocomposites for Hydrogels have broad applicability in the biomedical industry due to their tunable Polymers Biomedical Applications. physical and biochemical properties (Table1)[ 1–3]; however, their poor mechanical strength 2021, 13, 856. https://doi.org/ limits their uses to non-load bearing applications [4–9]. Therefore, the development of 10.3390/polym13060856 strategies to improve mechanical properties as well as add or improve other complementary properties has been a focus of research for the past few decades.
    [Show full text]
  • Nanostructured Hydrogels for Tissue
    Nanostructured Hydrogels for Tissue Engineering and Regenerative Medicine Kaivalya A Deo, Giriraj Lokhande, and Akhilesh K Gaharwar, Texas A&M University, College Station, TX, United States © 2019 Elsevier Inc. All rights reserved. Introduction 1 Metal and Metal-Oxide Nanoparticle Based Nanocomposite Hydrogels 2 Gold Nanoparticles 3 Silver Nanoparticles 4 Iron Oxide Nanoparticles 5 Alumina and Titania Nanoparticles 5 Zinc Oxide and Copper Oxide Nanoparticles 5 Carbon Nanomaterial Based Nanocomposite Hydrogels 5 Carbon Nanotubes (CNTs) 5 Nanodiamonds 6 Graphene and Graphene Oxide 6 Inorganic Nanomaterial Based Nanocomposite Hydrogels 6 Nanoclay and Nanosilicates 7 Calcium Phosphate and Hydroxyapatite Nanoparticles 9 Bioactive Glass Nanoparticles 9 Organic Nanomaterial Based Nanocomposite Hydrogels 9 Hyperbranched Nanoparticles and Dendrimers 10 Liposomes and Micelles 11 Emerging Trends and Future Outlook 11 Ordered Nanocomposite Hydrogels 11 Soft Robotics and Biosensors 11 Programmable Materials 12 Conclusion 12 Further Reading 13 Introduction In recent years there has been a demand for engineered functional tissues which would mimic body’s intricate tissue architecture and also maintain necessary microenvironment for cellular growth. A challenge for designing such functional tissue is the development of biomaterials with controlled mechanical, physical, chemical and biological properties. Among various biomaterials currently available hydrogels are the most suited for this application as they closely simulate native tissue present inside the body. Hydrogels consist of three-dimensional (3D) crosslinked polymeric network which can absorb large quantities of water, giving some unique properties to these materials. Hydrogels are synthesized from natural or synthetic polymers and possess various advantages over conventional organic and inorganic materials such as biodegradability, biocompatibility, processability, and functionalization.
    [Show full text]
  • Synthesis and Mechanical Properties of Double Cross-Linked Gelatin-Graphene Oxide Hydrogels
    This is a repository copy of Synthesis and mechanical properties of double cross-linked gelatin-graphene oxide hydrogels. White Rose Research Online URL for this paper: http://eprints.whiterose.ac.uk/114835/ Version: Accepted Version Article: Piao, Y. and Chen, B. (2017) Synthesis and mechanical properties of double cross-linked gelatin-graphene oxide hydrogels. International Journal of Biological Macromolecules, 101. pp. 791-798. ISSN 0141-8130 https://doi.org/10.1016/j.ijbiomac.2017.03.155 Reuse This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND) licence. This licence only allows you to download this work and share it with others as long as you credit the authors, but you can’t change the article in any way or use it commercially. More information and the full terms of the licence here: https://creativecommons.org/licenses/ Takedown If you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing [email protected] including the URL of the record and the reason for the withdrawal request. [email protected] https://eprints.whiterose.ac.uk/ Synthesis and Mechanical Properties of Double Cross-linked Gelatin- Graphene Oxide Hydrogels Yongzhe Piao, Biqiong Chen* Department of Materials Science and Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom *Corresponding author. Email: [email protected] Abstract Gelatin is an interesting biological macromolecule for biomedical applications. Here, double cross-linked gelatin nanocomposite hydrogels with incorporation of graphene oxide (GO) were synthesized in one pot using glutaraldehyde (GTA) and GTA-grafted GO as double chemical cross-linkers.
    [Show full text]
  • Gradient Nanocomposite Hydrogels for Interface Tissue Engineering Lauren M
    BASIC SCIENCE Nanomedicine: Nanotechnology, Biology, and Medicine 14 (2018) 2465–2474 Research Article nanomedjournal.com Special Topic: Two-Dimensional Biomaterials in Regenerative Medicine Gradient nanocomposite hydrogels for interface tissue engineering Lauren M. Crossa, Kunal Shaha, Sowmiya Palania, Charles W. Peaka, ⁎ Akhilesh K. Gaharwara,b,c, aDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, USA bDepartment of Materials Science and Engineering, Texas A&M University, College Station, TX, USA cCenter for Remote Health Technologies and Systems, Texas A&M University, College Station, TX, USA Received 23 November 2016; accepted 24 February 2017 Abstract Two-dimensional (2D) nanomaterials are an emerging class of materials with unique physical and chemical properties due to their high surface area and disc-like shape. Recently, these 2D nanomaterials have been investigated for a range of biomedical applications including tissue engineering, therapeutic delivery and bioimaging, due to their ability to physically reinforce polymeric networks. Here, we present a facile fabrication of a gradient scaffold with two natural polymers (gelatin methacryloyl (GelMA) and methacrylated kappa carrageenan (MκCA)) reinforced with 2D nanosilicates to mimic the native tissue interface. The addition of nanosilicates results in shear-thinning characteristics of prepolymer solution and increases the mechanical stiffness of crosslinked gradient structure. A gradient in mechanical properties, microstructures and cell adhesion
    [Show full text]
  • Antibacterial Properties and Ph Sensitive Swelling of Insitu Formed Silver-Curcumin Nanocomposite Based Chitosan Hydrogel
    polymers Article Antibacterial Properties and pH Sensitive Swelling of Insitu Formed Silver-Curcumin Nanocomposite Based Chitosan Hydrogel M. M. Abd El-Hady 1,2,* and S. El-Sayed Saeed 3 1 Department of Physics, College of Science and Arts, Qassim University, Al Asyah, P.O. Box 6666, Buraidah 51452, Saudi Arabia 2 National Research Centre, Textile Research Division, 33 El-Behoth Street, Dokki, P.O. Box 12622, Cairo 11461, Egypt 3 Department of Chemistry, College of Science, Qassim University, Buraidah, Saudi Arabia P.O. Box 6666, Buraidah 51452, Saudi Arabia; [email protected] * Correspondence: [email protected] Received: 24 September 2020; Accepted: 21 October 2020; Published: 23 October 2020 Abstract: A simple method was used to prepare curcumin/silver nanocomposite based chitosan hydrogel. In an alkaline medium, chitosan and chitosan nanocomposite hydrogels were prepared using the physical crosslinking method. The prepared hydrogels were stable for a long period at room temperature. In one step, silver nanoparticles were prepared insitu using silver nitrate solution and curcumin oxide within the hydrogel network formation. In the meantime, curcumin compound served as both a reducing and stabilizing agent. The structure and surface morphology of nanocomposite hydrogels were characterized by FTIR, SEM, and EDX analysis confirmed the formation of silver nanoparticles within the hydrogel network. Moreover, Images of TEM showed a spherical shape of silver nanoparticles with an average size of 2–10 nm within the matrix of the hydrogel. The formation mechanism of nanocomposite based hydrogel was reported. Besides that, the effect of chitosan and silver nitrate concentrations were studied.
    [Show full text]
  • Nanoengineered Injectable Hydrogels for On-Demand And
    NANOENGINEERED INJECTABLE HYDROGELS FOR ON-DEMAND AND LOCALIZED THERAPEUTIC DELIVERY A Thesis by NIMA A. JALILI Submitted to the Office of Graduate and Professional Studies of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Chair of Committee, Akhilesh K. Gaharwar Committee Members, Daniel L. Alge Melissa A. Grunlan Head of Department, Anthony Guiseppi-Elie August 2016 Major Subject: Biomedical Engineering Copyright 2016 Nima A. Jalili ABSTRACT “Smart” hydrogels are an emerging class of biomaterials that respond to multiple external stimuli and investigated for a range of biomedical applications, including therapeutic delivery, and regenerative engineering. Stimuli-responsive nanogels based on thermoresponsive polymers such as poly(N-isopropylacrylamide) (PNIPAM) and magnetic nanoparticles (MNPs), are developed as “smart carriers” for on-demand delivery of therapeutic biomolecules via magneto-thermal activation. However due to their small size and systemic introduction, these PNIPAM/MNPs nanogels result in limited control over long-term, localized therapeutic delivery. Here, we developed an injectable nanoengineered hydrogel loaded with PNIPAM/MNPs for localize on-demand delivery of therapeutics (doxorubicin (DOX)). We have engineered shear-thinning and self-recoverable hydrogels by modulating crosslinking density of our methacrylated gelatin (GelMA) network. PNIPAM/MNPs nanogels loaded with DOX were entrapped within the GelMA pre-polymer solution prior to crosslinking. The temperature and magnetic field dependent release of loaded DOX was observed from the nanoengineered hydrogels (GelMA/(PNIPAM/MNPs)). The in vitro efficacy of DOX released from injectable nanoengineered hydrogels was investigated using preosteoblast and osteosarcoma cells. Overall, these results demonstrated that the injectable nanoengineered hydrogels can be used for on-demand and localized therapeutic delivery for biomedical applications.
    [Show full text]