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Drosophila Rtf1 Functions in Histone Methylation, Gene Expression, and Notch Signaling

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Drosophila Rtf1 Functions in Histone Methylation, Gene Expression, and Notch Signaling Drosophila Rtf1 functions in histone methylation, gene expression, and Notch signaling Kristen Tenney*, Mark Gerber*, Anne Ilvarsonn*, Jessica Schneider*, Maria Gause*, Dale Dorsett*†, Joel C. Eissenberg*†, and Ali Shilatifard*†‡ *Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St. Louis, MO 63104; and †Saint Louis University Cancer Center, Saint Louis University School of Medicine, St. Louis, MO 63104 Edited by Mark T. Groudine, Fred Hutchinson Cancer Research Center, Seattle, WA, and approved June 19, 2006 (received for review May 3, 2006) The Rtf1 subunit of the Paf1 complex is required for proper monoubiquitination of histone H2B and methylation of histone H3 on lysines 4 (H3K4) and 79 in yeast Saccharomyces cerevisiae. Using RNAi, we examined the role of Rtf1 in histone methylation and gene expression in Drosophila melanogaster. We show that Dro- sophila Rtf1 (dRtf1) is required for proper gene expression and development. Furthermore, we show that RNAi-mediated reduc- tion of dRtf1 results in a reduction in histone H3K4 trimethylation levels on bulk histones and chromosomes in vivo, indicating that the histone modification pathway via Rtf1 is conserved among yeast, Drosophila, and human. Recently, it was demonstrated that histone H3K4 methylation mediated via the E3 ligase Bre1 is critical for transcription of Notch target genes in Drosophila. Here we demonstrate that the dRtf1 component of the Paf1 complex func- tions in Notch signaling. chromatin ͉ elongation ͉ RNA polymerase II ͉ transcription ͉ monoubiquitination Fig. 1. Rtf1 regulation of histone H3 methylation. Histone H3 methylation nterplay between transcriptional activators and repressors patterns in the indicated yeast strains were tested by using Western blot Iregulates gene expression by RNA polymerase II (RNA Pol analyses with specific antibodies generated toward each modified histone H3. II). In several cases, chromatin structure is implicated in tran- scriptional activation and repression. Posttranslational methyl- ation of lysines on the N-terminal tails of histones is thought to ylation and is critical for transcription of Notch target genes. In modulate higher-order chromatin folding and can activate or this study, we show that the dRtf1 component of the Paf1 repress transcription, depending on the residue being methyl- complex participates in Notch signaling in the wing margins. Our ated, the regulatory protein recruited by the methyl mark, and studies indicate that transcriptional regulation via the Paf1 whether the lysine is mono-, di-, or trimethylated (1). complex is highly conserved among eukaryotes. Histone modifications can be interdependent, such that one Results modification requires another preexisting modification (1). Hi- stone H3 methylation at lysines 4 and 79 is catalyzed by the Paf1 Complex Regulation of Histone Methylation. Many of the complex of proteins associated with Set1 (COMPASS) and subunits of COMPASS (the yeast homologue of the mammalian Dot1p, respectively in Saccharomyces cerevisiae (1–8). Methyl- MLL complex and the Drosophila trithorax complex) are re- ͞ ation at these residues requires monoubiquitination of histone quired for the proper mono-, di-, and or trimethylation of H3K4 H2B at lysine 123 by Rad6͞Bre1 (9–11). The Paf1 complex (1). In addition to COMPASS, the E2 conjugating enzyme Rad6 indirectly regulates histone methylation through its regulation of and its E3 ligase Bre1 are required for proper H3K4 methylation H2B monoubiquitination and interaction of COMPASS with via the regulation of H2B monoubiquitination (9–11). Also, it RNA Pol II (12–14). has been shown that deletion of components of the Paf1 complex ͞ The Paf1 complex in yeast is composed of five subunits, Paf1, and the Bur1 Bur2 kinase can greatly reduce histone H2B Rtf1, Cdc73, Ctr9, and Leo1, and is associated with the elon- monoubiquitination and, thereby, H3K4 methylation (12–14, 20, gating form of RNA Pol II (1, 15–18). The Rtf1 component of 21). However, deletion of RTF1, which is required for the Paf1 is required for H2B ubiquitination by Rad6 (12–14) and for activation of Rad6, seems to be required for mono-, di-, and the recruitment of Set1͞COMPASS to elongating RNA Pol II trimethylation mediated by COMPASS (Fig. 1). This observa- (12, 13). Because Rtf1 is essential for histone monoubiquitina- tion mirrors that of effects observed when either RAD6 or tion, methylation, and transcriptional control in yeast, we sought BRE1 is deleted. Although loss of H2B monoubiquitination is the Drosophila homologue dRtf1 to characterize its role in higher not fully required for H3K4 monomethylation by COMPASS, eukaryotes. Here we use RNAi to reduce dRtf1 expression levels this observation can be explained by the fact that Rtf1 is not only and examine the in vivo effect of dRtf1 reduction on transcription required for the regulation of H2B monoubiquitination but also and development in the fly. We show that RNAi knockdown of dRtf1 causes pupal lethality. To demonstrate a role for dRtf1 in gene expression, we tested the effect of dRtf1 RNAi knockdown Conflict of interest statement: No conflicts declared. on heat shock gene expression and found that Rtf1 knockdown This paper was submitted directly (Track II) to the PNAS office. results in a reduction in heat shock (Hsp70) gene expression. Abbreviation: RNA Pol II, RNA polymerase II. Recently, Bray et al. (19) demonstrated that the Drosophila ‡To whom correspondence should be sent at the * address. E-mail: [email protected]. homologue of Bre1 is required for proper histone H3K4 meth- © 2006 by The National Academy of Sciences of the USA 11970–11974 ͉ PNAS ͉ August 8, 2006 ͉ vol. 103 ͉ no. 32 www.pnas.org͞cgi͞doi͞10.1073͞pnas.0603620103 Downloaded by guest on September 27, 2021 Fig. 3. Loss of Rtf1 levels on chromatin. SympUAST–dRtf1 flies were crossed to the Actin5C–Gal4͞CyO, yϩ driver line to activate RNAi. Fixed polytene chromosome squashes were prepared from CyO (WT) (A) and Actin 5C (dRtf1 RNAi) (B) larvae and were immunostained with an antibody specific for dRtf1. Rtf1, dRtf1 (Fig. 2A). Fig. 2B shows dRtf1 mRNA levels visualized by Northern blot during eight developmental stages. A transcript of Ϸ3 kb was detected by using dRtf1-radiolabeled antisense RNA probe. Much higher levels of transcript were observed in embryos than in any other developmental stage (Fig. 2B). In this respect, dRtf1 expression was similar to elongation factor dELL (22), but it contrasted with elongation factor dEloA expression, which is most abundant in late larvae and during pupariation (23). dRtf1 Is Essential for Development in Drosophila. We used RNAi to lower Rtf1 mRNA and protein levels, to determine whether dRtf1 is required for Drosophila viability. RNAi knockdown of dRTF1 was accomplished by using P element-mediated trans- Fig. 2. Drosophila Rtf1 expression patterns. (A) The gene CG10955 was formation of the SympUAST vector with a 600-bp insertion of identified as the Drosophila homologue of Rtf1. (B) Total RNA was isolated dRtf1 cDNA sequence. The SympUAST vector contains two from each developmental stage of Drosophila. Fifteen micrograms of total convergent Gal4-inducible UAS promoters that can be used to RNA from each stage was loaded and analyzed by Northern blot analysis using transcribe double-stranded RNA from the dRtf1 fragment, ac- a dRtf1-specific probe. tivating the Drosophila RNAi machinery (24). SympUAST–dRtf1 flies were crossed to the Actin5C–Gal4͞CyO, yϩ driver line to activate RNAi. Rtf1 RNAi is expressed only in Actin5C–Gal4͞ plays a role in the recruitment of COMPASS to the transcribing SympUAST–dRtf1 progeny, and not their CyO, yϩ͞SympUAST– RNA Pol II (13). In addition to regulation of H3K4 methylation, dRtf1 siblings. When we used one line, 10A, 235 CyO, yϩ͞ different components of the Paf1 complex have varying effects SympUAST–dRtf1 adults were recovered with no Actin5C–Gal4͞ BIOLOGY on other types of H3 tail methylation (Fig. 1). SympUAST–dRtf1 progeny observed. When we used a second DEVELOPMENTAL Because Paf1 is required for histone H3K4 and K79 methyl- independent line, 17C, 310 CyO, yϩ͞SympUAST–dRtf1 adults ation, we tested the effect of Paf1 loss on histone methylation were recovered with no Actin5C–Gal4͞SympUAST–dRtf1 prog- stability in vivo (data not shown). We used a tetracycline- eny observed. A large number of dead pupae were observed in regulated PAF1 gene strain grown under normal conditions, both crosses, suggesting that the Rtf1 RNAi flies were dying turning off the expression of Paf1 in the presence of tetracycline. during the pupal stage. Because lethality was observed by using Cell extracts were prepared at different time points, and histone two independent SympUAST–dRtf1 insertion lines, lethality was H3 modification stability was tested in the absence of Paf1. After unlikely to be due to ectopic gene activation at the SympUAST– approximately 4 hours in the presence of tetracycline, Paf1 levels dRtf1 insertion site. were reduced by Ͼ95%. However, even 12 hours after Paf1 loss, In Actin5C–Gal4͞SympUAST–dRtf1 third instar larvae, Rtf1 histone H3K4 and K79 methylation levels appear to be unaf- protein levels on polytene chromosomes (Fig. 3B) were reduced fected. Our observation substantiates a report by Ng et al. (14), compared with those in their CyO, yϩ͞SympUAST–dRtf1 siblings who found that H3K4 methylation seems to be a stable mark (Fig. 3A). Although Northern blot analysis showed that dRtf1 once established
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