applied sciences Article Plasma-Treated Flammulina velutipes-Derived Extract Showed Anticancer Potential in Human Breast Cancer Cells 1, 1, 2, 1 Sarmistha Mitra y, Pradeep Bhartiya y, Neha Kaushik y , Linh Nhat Nguyen , Rizwan Wahab 3, Sander Bekeschus 4 , Eun Ha Choi 1,* and Nagendra Kumar Kaushik 1,* 1 Plasma Bioscience Research Center/Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Korea;
[email protected] (S.M.);
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[email protected] (L.N.N.) 2 College of Engineering, Department of Biotechnology, University of Suwon, Hwaseong 18323, Korea;
[email protected] 3 College of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi Arabia;
[email protected] 4 Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for Plasma Science and Technology (INP Greifswald), 17489 Greifswald, Germany;
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[email protected] (N.K.K.) These authors equally contributed to this work. y Received: 9 October 2020; Accepted: 22 November 2020; Published: 25 November 2020 Abstract: Natural products with medicinal properties are among alternative therapies of interest due to their high body tolerance. We aimed to determine whether nonthermal gas plasma could enhance the medicinal value of Flammulina velutipes mushrooms. Generated gas plasma was characterized by its emission spectrum in ambient air, pH, temperature, and H2O2 and NOx concentrations after exposure for various periods. Phenolic and flavonoid contents in the extracts were measured using antioxidant assays and Fourier transform infrared and ultraviolet-visible spectroscopy.