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JOINT EVENT 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain Scientific Tracks & Abstracts Day 1
Pharmacology 2018 & Chromatography-HPLC Congress 2018
Page 41 Sessions: Day 1 August 02, 2018 Analytical and Bioanalytical Applications of Chromatography-HPLC | Biochemical Applications of Chromatography-HPLC | Chromatography-HPLC in Bio-Medical research | Chromatography-HPLC in Food Sciences Chromatography-HPLC in Pharmaceutical Analysis
Session Chair Clydewyn M Anthony The United States Pharmacopeial Convention (USP), USA
Session Introduction Title: Monolithic chromatographic supports employed in virus separation and purification Mladen Krajacic, University of Zagreb, Croatia Title: Immobilized artificial membrane chromatography as a tool in medicinal chemistry and in environmental sciences Fotios Tsopelas, National Technical University of Athens, Greece Title: The study of naproxen desorption from the silica by RP-HPLC Monika Šuleková, University of Veterinary Medicine and Pharmacy in Košice, Slovakia Title: Chiral HPLC resolution of a potentially serious global health crisis Clydewyn M Anthony, The United States Pharmacopeial Convention (USP), USA
Page 42 Mladen Krajacic, Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Monolithic chromatographic supports employed in virus separation and purification Mladen Krajacic University of Zagreb, Croatia
he particular structure of monolithic chromatographic supports has been proved highly advantageous in analytic separation Tand purification of large biomolecules. Characterized by very high porosity, high binding capacity, and high flow rate based on convective mass transport, monoliths are particularly applicable in virus research. Monolith chromatography has hardly any limitation to be applied in processing of virus particles, huge macromolecular complexes, and viral genomes, especially when being distinct from nucleic acid forms present in the host cell. The majority of trials published so far, have exploited ion exchange carriers, although other chemistries are also applicable, like hydrophobic interaction when concentrating viruses from marine environment. According to papers published over the past decade, viruses of different sizes, structures and morphologies, even virus-like particles, have successfully been purified from tissue homogenates or cell lysates. Moreover, following virtual separation by overlapping chromatograms obtained from separate experiments, a real separation of three virus species, and a distant strain of one of them (four viral fractions in total) was accomplished from laboratory prepared mixture. One of the most recent achievements is a proof of principle that virus chromatographic feature could easily be modified without abolishing its structural stability, or its biological activity. As adenoviruses have been used in almost a quarter of human gene therapy trials, the experiment was conducted with an adenoviral vector. The deletion of just two negatively charged amino acids from the main structural protein was efficient in shortening chromatographic retention of the recombinant adenovirus. In this way, it would be possible to shift virus particles away from particular interfering substances present in the crude lysate. The concept might be followed to facilitate chromatography-approach purification by engineering modifications contributing to virus separability, besides those contributing to its therapeutic functionality.
Figure: Shortened retention time of both the unassembled hexon protein (E3) and the virion fraction (E5) obtained for hexon-modified adenoviral vector. The shifted Ad5 recombinant missed just two negatively charged amino acids in the hexon protein compared to original virus particle displaying wild-type capsid. Following short low-speed centrifugation and filtration, the cell lysate was loaded to QA monolithic support with 20 mM Tris, 5% glycerol, pH 7.5, and eluted with a gradient of loading/eluting buffer, where the eluting buffer additionally contains 2 M NaCl. The flow rate was 2 mL/min. Recent Publications 1. Krajacic M, Ravnikar M, Strancar A and Gutiérrrez-Aguirre I (2017) Application of monolithic chromatographic support in virus research. Electrophoresis 38:2827-2836. 2. Ruscic J, Ambriovic-Ristov A, Majhen D, Kolundzija S, Barut M, Benihoud K and Krajacic M (2016) Manipulating adenoviral vector ion-exchange chromatography: Hexon versus fiber. Journal of Separation Science 39:4299-4304. 3. Ruscic J, Gutiérrrez-Aguirre I, Tusek Znidaric M, Kolundzija S, Slana A, Barut M, Ravnikar M and Krajacic M (2015) A new application of monolithic supports: The separation of viruses from one another. Journal of Chromatography A 1388:69-78. 4. Ruscic J, Gutiérrrez-Aguirre I, Urbas L, Kramberger P, Mehle N, Skoric D, Barut M, Ravnikar M and Krajacic M (2013) A novel application of methacrylate based short monolithic columns: Concentrating Potato spindle tuber viroid from water samples. Journal of Chromatography A 1274:129-136.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 43 conferenceseries.com JOINT EVENT 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
5. Krajacic M, Ivancic-Jelecki J, Forcic D, Vrdoljak A and Skoric D (2007) Purification of plant viral and satellite double- stranded RNAs on DEAE monoliths. Journal of Chromatography A 1144:111-119.
Biography Mladen Krajacic is a Full Professor at University of Zagreb where he completed his PhD in Virology. As a Head of the Laboratory and Principal Investigator in several research projects, he has been leading the molecular biology investigations on viruses, sub-viral agents (viroids, satellite RNAs) and non-cultivable bacteria. During the last decade, he has dedicated to chromatography on monolithic supports and its appliance in separation of viral and sub-viral ribonucleic acids, as well as virus particles. His results have been published in prominent scientific journals in which he has regularly been employed as a Reviewer. He was supervising a number of young research fellows who have received positions from well recognized institutions across the world.
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Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 44 Fotios Tsopelas et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Immobilized artificial membrane chromatography as a tool in medicinal chemistry andin environmental sciences Fotios Tsopelas1, C Stergiopoulos1, P Nikolaou2, M Ochsenkühn-Petropoulou1 and A Tsantili-Kakoulidou3 1National Technical University of Athens, Greece 2University of Peloponnese, Greece 3University of Athens, Greece
idgeon and his coworkers described for the first time in 1989, the immobilization of phosphatidylcholine to propylamino- Psilica skeleton and up to now immobilized artificial membrane (IAM) chromatography have been employed for simulation of the environment of cell membranes. In particular, IAM chromatography constitutes a valuable tool for medicinal chemists for prioritization of drug candidates in the early drug development stages. The retention outcome on IAM stationary phases encodes lipophilicity, electrostatic and other secondary interactions in contrary to traditional octanol-water partitioning.
An increasing number of publications in recent years suggest that IAM indices, including isocratic logk(IAM) or extrapolated
logkw(IAM) retention factors, hydrophobicity index CHI(IAM) which corresponds to the percentage of acetonitrile required for
equal partitioning of the solute between mobile and stationary phase (i.e. logk=0) or the polarity parameter ∆logkw(IAM) can successfully model the passage of xenobiotics through biological membranes and barriers and predict pharmacokinetic properties, often in combination with additional descriptors. More recently, IAM chromatography is applied to estimate toxicological endpoints in regard to drug safety, such as the phospholipidosis potential, or in regard to chemicals risk hazard including the bio-concentration factor and aquatic organisms’ toxicity. The presentation will be devoted to applications of IAM chromatography to medicinal chemistry and environmental sciences. Examples referring to modeling of human oral
absorption, blood-brain penetration, skin partition as well as bioconcentration factor and median toxicity (LC50) in aquatic organisms will be discussed. The combination of promising results in both medicinal chemistry and in environmental science with the speed, reproducibility and low analyte consumption suggest that a broader application of IAM chromatography in early drug discovery process and in ecotoxicity is expected in initial drug candidate selection and contribute to reduced risk hazard of chemicals.
Recent Publications 1. Tsopelas F, Malaki N, Vallianatou T, Chrysanthakopoulos M, Vrakas D, Ochsenkühn-Petropoulou M and Tsantili- Kakoulidou A (2015) Insight into the retention mechanism on immobilized artificial membrane chromatography using two stationary phases. Journal of Chromatography A 1396:25-33. 2. Tsopelas F, Vallianatou T and Tsantili-Kakoulidou A (2016) The potential of immobilized artificial membrane chromatography to predict human oral absorption. European Journal of Pharmaceutical Sciences 81:82-93. 3. Tsopelas F, Vallianatou T and Tsantili-Kakoulidou A (2016) Advances in immobilized artificial membrane (IAM) chromatography for novel drug discovery. Expert Opinion on Drug Discovery 11:473-488. 4. Tsopelas F, Giaginis C and Tsantili- Kakoulidou A (2017) Lipophilicity and biomimetic properties to support drug discovery. Expert Opinion on Drug Discovery 12:885-896.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 45 conferenceseries.com JOINT EVENT 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
5. Tsopelas F, Tsagkrasouli M, Poursanidis P, Pitsaki M, Vasios G, Danias P, Panderi I, Tsantili- Kakoulidou A and Giaginis C (2018) Retention behavior of flavonoids on immobilized artificial membrane chromatography and correlation with cell- based permeability. Biomedical Chromatography 32:1-11.
Biography Fotios Tsopelas is a Lecturer in the School of Chemical Engineering at National Technical University (NTUA), Greece. He studied Chemical Engineering in the NTUA (1999) and Pharmacy (2004) in the National and Kapodistrian University of Athens. He completed his PhD in 2007 in Environmental Analytical Chemistry at NTUA (scholarship from Onassis Foundation) and his Postdoctoral research was focused on biomimetic chromatography for novel drug design. He has more than 25 publications in peer-reviewed scientific journals and more than 40 contributions in international conferences. He has acted as a Referee in more than 20 international journals. He has participated as a member of scientific committee in two international conferences. He has coordinated five national and international funded research projects. His research interest is mainly focused on the development of biomimetic chromatographic approaches for the evaluation of pharmacokinetic properties of candidate drugs and ecotoxicity of emerging pollutants.
Notes: Supported by the Onassis Foundation under the ‘‘Special Grant and Support Program for Scholars’ Association Members” (Grant No. R ZN 004-1/2017-2018).
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 46 Monika Šuleková, Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
The study of naproxen desorption from the silica by RP-HPLC Monika Šuleková University of Veterinary Medicine and Pharmacy in Košice, Slovakia
aproxen, a non-steroidal anti-inflammatory drug (NSAID) is widely used to moderate pain relief in the treatment of many diseases. NNaproxen has analgesic and antipyretic properties. Mesoporous silica SBA-15 was prepared to evaluate its application as naproxen drug delivery system. The amount of naproxen released from the pores of mesoporous silica SBA-15 into the solutions was determined by the method of a reverse-phase high performance liquid chromatography (RP-HPLC). SBA-15 having 3-aminoprophyl-, methyl-, fenyl- and cyclohexyl-surface groups was successfully prepared by the grafting of SBA-15 with the corresponding alkoxysilanes. The release of the drug was performed in two different media, in a simulated body fluid (pH 7.40) and in a simulated gastric fluid (pH 2.06). The HPLC system Dionex Ultimate 3000 RS (Thermo Fisher Scientific, Germany) consisted of a quaternary pump, a degasser, an automated injector, a column oven and a diode array detector DAD. HPLC system was used, with stationary phase ODS Hypersil C18 column (150x4.6 mm, 3 μm). To determine the concentration of naproxen, the calibration curve has been established based on five solutions of different concentrations of naproxen. The linearity was determined by threefold repeating measurement of each concentration step. The mixture of acetonitrile and water (55:45, v/v) adjusted with ortho-phosphoric acid to pH 3 was selected as the best mobile phase. The flow rate was 1 mL/min and detection was carried out at a wavelength of 229 nm. During the chromatographic separation, the mobile phase was kept isocratic. The release of the drug was studied as a function of time and the results are shown in Figure.
Figure: Chromatograms of naproxen released from the silica SBA-15 modified by methyl group in simulated gastric fluid pH 2.06 (A) and in a simulated body fluid pH 7.40 (B) Recent Publications 1. Reitznerová A, Šuleková M, Nagy J, Marcinčák S, Semjon B, Čertík M and Klempová K (2017). Lipid peroxidation process in meat and meat products: a comparison study of malondialdehyde determination between modified 2-thiobarbituric acid spectrophotometric Method and reverse-phase high-performance liquid chromatography. Molecules DOI: 10.3390/ molecules22111988. 2. Šuleková M, Smrčová M, Hudák A, Heželová M and Fedorová M (2017) Organic colouring agents in the pharmaceutical industry. Folia Veterinaria 61(3):32-46. 3. Šuleková M, Hudák A and Smrčová M (2016) The determination of food dyes in vitamins by RP-HPLC. Molecules 21(10):1368. 4. Šuleková M and Hudák A (2015) Determination of the colorants in vitamin E by HPLC with photodiode array detection. Čes. slov. Farm. 64(6):279. 5. Telepčáková M, Andruch V and Balogh IS (2005) Indirect extraction-spectrophotometric determination of chromium. Chem. Pap. 59(2):109.
Biography Monika Šuleková has completed her Graduation at the University of Pavol Josef Šafárik in Košice, Slovakia. During her university studies, she spent half a year at Friedrich-Schiller University in Jena, Germany where she studied Analytical Chemistry. Nowadays she works as a Teacher at the University of Veterinary Medicine and Pharmacy in Košice, Slovakia as well as a Researcher in the field of desorption of drugs from mesoporous silica modified by different functional groups, and in determination of synthetic dyes in pharmaceutical products by the RP-HPLC method.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 47 Clydewyn M Anthony et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Chiral HPLC resolution of a potentially serious global health crisis Clydewyn M Anthony, Earl Jones Jr, Eduardo Lim, Minli Lu, Jeffrey Palombo, Shane Tan and Leonel Santos United States Pharmacopeial Convention, USA
few years ago there was a potentially serious health issue in Pakistan and Paraguay which spurred investigation and Aresponsive action by both the United States Pharmacopeial Convention (USP) and The Food and Drug Administration (FDA). This crisis resulted in the deaths of adults and children who had ingested Dextromethorphan Cough Syrup. It was later determined and confirmed that toxic levels of the controlled substance, levomethorphan, an enantiomer of dextromethorphan, was found in the drug formulation and was responsible for the resulting deaths. USP has thus charged with developing a quantitative procedure for monitoring levomethorphan and simultaneously incorporating this method as a revision to the documentary standard within its compendium. At the time of the public health issue, the existing USP Dextromethorphan monograph did not include a quantitative procedure for the determination of its enantiomer, levomethorphan. Hence a chiral HPLC method was developed to bring the monograph up-to-date and simultaneously address obvious safety concerns associated with the enantiomer. The proposed HPLC method separates levomethorphan and dextromethorphanone (another impurity, dextromethorphan Related Compound C) from dextromethorphan; and allowed quantitation to satisfy acceptance criteria requirements for these impurities (0.10%). Hence, manufactured lots which test higher than the specified limit of levomethorphan can be rejected thus helping to prevent potential safety issues in the future. A complete overview of the issues encountered in the development of this chiral HPLC method along with the challenges associated with the implementation of a global procedure which utilizes a schedule 11 controlled substance as a public standard will be presented.
Biography Clydewyn M Anthony is a Senior Scientific Liaison in the Chemical Medicines Division at The United States Pharmacopeial Convention (USP) and is currently responsible for the modernization of documentary standards for Over-The-Counter drug formulations. He completed his PhD in Analytical Chemistry at The Pennsylvania State University and BS in Chemistry in Hunter College at the City University of New York. Prior to joining USP in May 1999, he worked as a Research Chemist with Texaco Inc. where he was responsible for performing and overseeing the compositional analyses of motor oils, gasolines and all aftermarket petroleum related products on both the domestic and international markets. He also held the position of Criminalist with the New York Police Forensic Investigation Department, worked on thousands of narcotics and arson related cases, and testified as an Expert Witness on behalf of the New York City Police Department.
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Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 48 Sheila Leone et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Effects of central RVD-Hemopressin (α) adminitrationon anxiety, feeding behavior and hypothalamic neuromodulators in the rat Sheila Leone1, Lucia Recinella1, Annalisa Chiavaroli1, Claudio Ferrante1, Adriano Mollica1, Giorgia Macedonio1, Azzurra Stefanucci1, Marilisa Pia Dimmito1, Szabolcs Dvorácskó2, Csaba Tömböly2, Giustino Orlando1 and Luigi Brunetti1 1“G. d’Annunzio” University, Italy 2Biological Research Centre of the Hungarian Academy of Sciences, Hungary
he endocannabinoid (eCB) system plays an important role in mood disorders, as such anxiety and depression. In addition, Tthe eCB system is involved in the regulation of food intake, metabolism and calorie storage. However, it is well known that the first generation of CB1 blockers designed to reduce food intake and body weight, such as Rimonabant, was discontinued due to psychiatric disorders, such as anxiety and depression. The discovery of RVD-hemopressin(α) [RVD-hp(α)], an hemoglobin α chain-derived peptide, revealed a promising research field for the pharmacotherapy of obesity. RVD-hp(α) was found to bind CB1 receptors, as negative allosteric modulator and as a positive allosteric modulator of CB2 receptor. In addition, pharmacological evidences reported a possible link between brain hypocretin/orexin, monoamine and eCB systems, as regards appetite and emotional behavior control. Considering this, the aim of our work was to investigated the effects of RVD-hp(α) on anxiety like behavior and food intake after central administration and related it to monoamine levels, proopiomelanocortin (POMC) and orexin-A gene expression, in the hypothalamus. We have studied the effects of central RVD-hp(α) (10 nmol) injection on anxiety-like behavior and feeding using different behavioral tests. Hypothalamic levels of norepinephrine (NE), dopamine (DA) and serotonin (5--HT) and gene expression of orexin-A and POMC were measured by high performance liquid chromatography (HPLC) and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis, respectively. Central RVD-hp(α) administration decreased locomotion activity and stereotypies. Moreover, RVD-hp(α) treatment inhibited anxiogenic-like behavior and food intake, NE levels and orexin-A gene expression, in the hypothalamus. Concluding, in the present study we demonstrated that central RVD-hp(α) induced anxiolytic and anorexigenic effects possibly related to reduced NE and orexin-A and POMC signaling, in the hypothalamus. These findings further support the central role of the peptide in rat brain thus representing an innovative pharmacological approach for designing new anorexigenic drugs targeting eCB system. Recent Publications 1. Leone S, Recinella L, Chiavaroli A, Martinotti S, Ferrante C, Mollica A, et al. (2017) Emotional disorders induced by Hemopressin and RVD-hemopressin(α) administration in rats. Pharmacol Rep.69:1247–1253. 2. Ferrante C, Recinella L, Leone S, Chiavaroli A, Di Nisio C, Martinotti S, et al. (2017) Anorexigenic effects induced by RVD- hemopressin(α) administration. Pharmacol Rep. 69:1402–1407. 3. Rubino T, Zamberletti E, Parolaro D (2015) Endocannabinoids and Mental Disorders. Handb Exp Pharmacol. 231:261–83. 4. Gatta-Cherifi B, Cota D (2016) New insights on the role of the endocannabinoid system in the regulation of energy balance. Int J Obes (Lond). 40:210–9. 5. Dodd GT, Mancini G, Lutz B, Luckman SM. (2010) The peptide hemopressin acts through CB1 cannabinoid receptors to reduce food intake in rats and mice. J Neurosci 30:7369–76. Biography Sheila Leone had completed Medicine & Surgery degree and Post-graduated in Clinical Pharmacology from University of Modena and Reggio Emilia (Modena, Italy) She worked as assistant professor of Pharmacology (BIO/14), Department of Pharmacy from University of Chieti “G. D’Annunzio” (Chieti, Italy). The research activity is mainly focus on neuropharmacology, cardiopharmacology, endocrinology and behavior of synthetic and vegetal drugs. Department of Pharmacy, University “G.d’Annunzio” Chieti- Pescara (Chieti, Italy). Author of papers (for the most part in international journals) and communications at National and International Congresses.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 50 Maria Laura Zuccoli et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
The acute effect of cannabis on plasma, liver and brain ammonia dynamics Maria Laura Zuccoli1, 2, Allan Barnes2, Lifeng Zhang2, Marylin Huestis2, 3, Da-Ting Lin2 and Osama A Abulseoud2 1University of Genoa, Italy 2National Institute on Drug Abuse, USA 3University of Maryland, USA
annabis is a psychoactive substance widely used for both medicinal and recreational purposes. Cannabis-induced acute Ccognitive and motoric side effects are common and remain without effective treatment. A better understanding of the neurobiological mechanisms underlying cannabis-related effects is urgently needed to facilitate the discovery of novel therapeutics. We recently observed elevation in ammonia plasma concentrations during and after oral, smoke or vapor cannabis controlled administration (54mg) to 15 healthy cannabis users. Cannabis produced significant time (p<0.05) and treatment (p<0.001) effects, with differences in plasma ammonia between placebo and edible cannabis administration at 30 (p<0.05) and 90min (p<0.05), between placebo and vaporized (p<0.05) and smoking routes (p<0.05) at 90min. Plasma ammonia concentration positively correlated with blood -9-tetrahydrocannabinol (THC) concentrations (p<0.05). Using a translational approach, we then examined the acute effect of THC on plasma, liver and brain ammonia concentrations and enzyme activity in different brain regions of mice at 1, 3, 5 and 30min after a single THC injection. We found significant reduction in striatal glutamine synthetase (GS) activity (p<0.05) and increase in striatal ammonia concentration (p<0.05) 5min post-injection. THC plasma concentration correlated positively with striatal ammonia (p<0.001) and negatively with striatal GS activity (p<0.05). At 30min, we found marked increase in striatal ammonia (p<0.001), associated with significant increase in plasma ammonia (p<0.05). For the first time, we demonstrate that plasma ammonia concentration increases after controlled cannabis administration and we provide evidence that this increase could be generated in the brain through suppression of striatal GS activity. Recent Publications 1. Rangroo Thrane V, Thrane A S, Wang F, Cotrina M L, Smith N A, et al. (2013) Ammonia triggers neuronal disinhibition and seizures by impairing astrocyte potassium buffering. Nat Med. 19(12):1643–8. 2. Eid T and Lee T S (2013) Reassessing the role of astrocytes in ammonia neurotoxicity.Nat Med. 19(12):1572–4. 3. Bloor R N, Wang T S, Spanel P, Smith D (2008) Ammonia release from heated 'street' cannabis leaf and its potential toxic effects on cannabis users. Addiction 103:1671–1677. 4. Landfield P W, Cadwallader L B and Vinsant S (1988) Quantitative changes in hippocampal structure following long-term exposure to delta9-tetrahydrocannabinol:possiblemediationbygluco-corticoid systems. BrainRes. 443:47–62.
Biography Maria Laura Zuccoli obtained my medical degree from the University of Genoa, Italy, and I completed the residency in Clinical Toxicology at the University of Florence, in Italy. I am currently a PhD candidate in Experimental Medicine at the University of Genoa and a Visiting Fellow at the National Institute on Drug Abuse, in Baltimore, USA. My main research topics focus on the understanding of neurobiological mechanisms involved in the effects of widely abused substances such as cannabinoids, opioids and alcohol, using in vivo two-photon endomicroscopy, optogenetic and chemogenetic behavioral assays and molecular biology techniques.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 51 Edith Bianchi, Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
S2T – A critical pillar & enabler of drug delivery patch pump Edith Bianchi Pronat Medical, Israel
ew approaches to drug delivery are a fast growing field in the medical device industry. Strong market forces and Nhealthcare trends are dramatically enhancing this evolution. Pharmacological advancements in drugs developments and their manufacturing have a tremendous contribution to the drug delivery field. One meaningful drive is the significant rise of injectable biologic therapies for more and more chronic conditions. The challenges presented by these drugs, are creating the need and opportunity for drug delivery systems. The personalized medicine trend is obviously influencing drug related topics. The changes in approach to the patient are shifting hospital care to homecare setting, and simultaneously, shifting administrating injection of drugs subcutaneously instead of intravenous. Obviously intravenous injection demands costly skilled staff and mostly cannot be done in home setting. These are all taking part too in the evolution of drug delivery devices. Another meaningful factor is patient's non-compliance to treatment, which is not only effecting the patient's health and poor outcomes, but is also baring substantial losses to pharmaceutical companies and resulting in a serious burden to the healthcare system. We are therefore, witnessing a great interest in adequate solutions for wearable drug delivery advanced devices which adhere to the patient's skin. When designing such a system, some significant challenges arise. The development of a drug delivery patch device involves critical issues such as safety, the drug/reservoir interaction and many other elements which are necessary to be considered. However, engineering teams are becoming more and more familiar with yet another critical aspect- Human Factor and Ergonomics. These factors are most crucial with any S2T ("Skin to Thing") solution, and have an even more crucial weight when dealing with a drug delivery patch. The "Skin to Thing" medical patch which is required to adhere the device to the body is indeed the body/machine crucial interface and should be given just as much a careful and thorough attention as the device itself, in order to achieve the device goals, assure the patient's compliance and successful treatment.
Biography Edith Bianchi is a senior Global Business Development expert, specializing in the Medical device industry – specifically in Digital Health and Wearable applications. She has an impressive and quite extraordinary multi-disciplinary background and qualifications. Those are combining both clinical record as a CRA and Registered Nurse with CICU experience, as well as strong business management orientation with an MBA degree from University of Derby. Ms. Bianchi has a long track record in Medical Device sales organizations and was a key person in few meaningful strategic cooperations between major players in the industry.
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Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 52 Ali Shaharki et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Investigation on the effects of hydroalchoholic extract of Levisticum officinale on cGMP levels and PDE5 gene expression in breast cancer cell lines MCF-7 and MDA-Mb-468 Ali Shaharki1, Marzieh Lotfian Sargazi2 and Ramin Saravani2 1University of Sistan and Baluchestan, Iran 2Zahedan University of Medical Sciences, Iran
ncreased expression of cyclic guanosine 3', 5' monophosphate phosphodiesterase-5 (cGMP-PDEs) mRNAs has been Idemonstrated in several human carcinomas, including breast carcinoma, pancreatic cancer, bladder cancer, squamous cell carcinoma and prostate cancer, in comparison to adjacent normal tissues. Studies have elucidated that an increase in intracellular cGMP induces apoptosis and decreases cell population growth. Therefore, selective inhibitors of these PDEs might be potential anticancer agents. Previous investigations have demonstrated that Levisticum officinalecontains flavonoids and alkaloids compound that are known as antitumoral components. The purpose of this study was to investigate the effect of Levisticum officinale hydroalcoholic extract on phosphodiesterase 5 gene expression, cGMP signaling pathway and its role in inducing apoptosis in the MCF-7(ER+) and in MDA-Mb-468 (triple-negative) cell lines. The mean inhibitory concentration (IC50) of extract was determined in both cell lines using of MTT assay, and the type of cell death was detected by flow cytometry. The expression of PDE5 and cGMP levels was measured by real-time polymerase chain reaction and colorimetric
assay, respectively. Treatment with hydroalchoholic extract of Levisticum officinale showed that 200 µg/ml to be the IC50 for
both cell lines. 12 hour treatment with IC50 dosage showed a maximum decrease in the PDE5 expression and maximum increase in intracellular cGMP level, although, these effects were more significant in MDA-MB-468. In conclusion, our results showed that hydroalchoholic extract of Levisticum officinale had an anti-proliferative and apoptotic effect in MCF-7 and MDA- Mb-468 targeting PDE5 and cGMP signaling pathway.
Figure 1: Effect of hydroalchoholic extract of L. officinale in inhibition of cell growth of breast cancer MCF-7 and MDA-Mb-468 cell lines. Cells were treated with different concentration of extract for 24, 48 and 72h. Proliferation was measured with MTT assay. Extract reduced cell proliferation in a time and dose-dependent manner. Results are presented as Mean±SD of three experiment. **P<0.01 compared to untreated control group. Recent Publications 1. Saravani R Galavi H R and Shahraki A (2017) Inhibition of phosphodiesterase 5 and increasing the level of cyclic guanosine 3', 5' monophosphate by hydroalchoholic Achillea wilhelmsii C. Koch extract in human breast cancer cell lines MCF-7 and MDA- Mb-468. Breast Cancer: Basic and Clinical Res. 1–7. 2. Afarnegan H, Shahraki A and Shahraki J (2017) The hepatoprotective effects of aquatic extract of L. officinale against paraquat hepatocyte toxicity. Pak J Pharm Sci 30:2363–2368. 3. Lagzian M, Shahraki A, Besharatian M and Asoodeh A (2017) A thermostable alkaliphilic protein-disulfide isomerae from bacillus subtilis DR8806: cloning, expression, biochemical characterization and molecular dynamics simulation. Int J Biol Macromol. 09:033. 4. Sargazi S, Saravani R, Galavi H R, et al. (2017) Effect of Levisticum officinale hydroalcoholic extract on DU-145 and PC-3 prostate cancer cell lines. Gene Cell Tissue 4(4):e66094.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 53 conferenceseries.com JOINT EVENT 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
5. Galavi H R, Saravani R, Shahraki A and Ashtiani M (2016) Anti-proliferative and apoptosis inducing potential of hydroalchoholic Achillea wilhelmsii C. Koch extract on human breast adenocarcinoma cell lines MCF-7 and MDA-Mb-468. Pak J Pharm Sci 29: 2397–2403.
Biography Ali Shahraki has his expertise in Teaching and Research at the University of Sistan and Baluchestan, Zahedan, Iran. He supervised more than 25 theses of Biochemistry for Master’s Degree of Science students. He works in the fields of neuroscience, herbal medicine and signal pathways for inducing anti-proliferative and apoptosis in cancer cell lines through medicinal plants. He has built this model after years of experience in research, evaluation, teaching and administration in universities and education institutions.
Notes:
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 54 Piotr Wojciechowski et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Neuropeptide FF diminishes the number of apnoeas and cardiovascular effects produced with systemic administration of endomorphin-1 Piotr Wojciechowski and Katarzyna Kaczyńska Mossakowski Medical Research Centre, Polish Academy of Sciences, Poland
europeptide FF (NPFF) behaves as an endogenous opioid-modulation peptide that seems to be involved in opiate-induced Ntolerance and dependence and is thought to present strong inhibitory interaction with opioids. Although pharmacological properties of NPFF on pain perception and opioid-induced tolerance seem to be well documented, its effect on breathing pattern and respiratory depression induced by opioids remains unclear. The aim of the present study was to examine an impact of intravenous NPFF injection on respiration and its potency in elimination post-opioid apnoea. Experiments were performed on urethane-chloralose anaesthetized male Wistar rats breathing spontaneously room air. Respiratory parameters, arterial blood pressure and heart rate were measured. Rats were treated with an intravenous injection of NPFF in four doses: 0.1; 0.3; 0.6 and 1.2 mg/kg. Two minutes later, a dose of endomorphine-1 (EM-1) (50 μg/kg) was administered. Systemic injection of NPFF in doses: 0.1–0.6 mg/kg evoked only dose dependent hypertensive effect. The highest dose caused also a short-lived reduction in tidal volume, which affected minute ventilation immediately after the challenge and evoked a single episode of apnoea in one rat lasting 3.7 s. Bolus injection of 50 μg/kg of endomorphin-1 was administered into the femoral vein of control animals without NPFF pre-treatment evoked apnoea in 5 of 6 rats of mean duration of 11.2±1.2 s, short-lived hypotension and a slow down in the heart rate. Pre-treatment with NPFF diminished the number of post-endomorphin-1 apnoeas, to 2 in 5 rats at a dose of 0.1–0.3 mg/kg and to 1 in 5 animals at a dose of 0.6 mg/kg. EM-1 induced hypotensive effects and decrease in the heart rate were also reduced at all tested doses of NPFF. Our experiments showed that stimulation of neuropeptide FF receptors in the periphery diminishes the number of EM-1-induced arrests of breathing, as well as its hypotensive effect. Recent Publications 1. Li N, Han Z L, Wang Z L, Xing Y H, Sun Y L, et al. (2016) BN-9, a chimeric peptide with mixed opioid and neuropeptide FF receptor agonistic properties, produces nontolerance-forming antinociception in mice. Br J Pharmacol. 173(11):1864–80. 2. Lin Y T, Kao S C, Day Y J, Chang C C and Chen J C (2016) Altered nociception and morphine tolerance in neuropeptide FF receptor type 2 over-expressing mice. Eur J Pain. 20(6):895–906. 3. Ayachi S and Simonin F (2014) Involvement of mammalian RF-amide peptides and their receptors in the modulation of nociception in rodents. Front Endocrinol (Lausanne) 5:158. 4. Moulédous L, Mollereau C and Zajac J M (2010) Opioid-modulating properties of the neuropeptide FF system. Biofactors 36(6):423–9.
Biography Piotr Wojciechowski is an Assistant in the Department of Respiration Physiology, Mossakowski Medical Research Centre, Warsaw, Poland. His interests scope within the crosstalk and interaction between neuropeptide and opioid systems and their effects on cardio-respiratory pattern.
Notes: "Research supported by National Science Centre MINIATURA 1 program 2017/01/X/NZ4/00465"
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 55 Nermeen Salah El Deen et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Study of the effect of different phosphodiesterase (1, 3, 5) inhibitors on diclofenac-induced acute renal failure in rats Nermeen Salah El Deen, Walaa Wadie and Hesham Aly Salem Cairo University, Egypt
iclofenac is one of the most commonly used NSAID that leads to severe adverse effects on the kidneys. The aim of the Dpresent study is to investigate the potential pretreatment effect of phosphodiesterase 1, 3 and 5 inhibitors on diclofenac- induced acute renal failure in rats. Rats were classified into five groups (n=8); control group (received 1 ml saline), model group (received diclofenac; 15 mg/kg, i.p), vinpocetine group (received vinpocetine; 20 mg/kg, P.O), cilostazol group (received cilostazol; 50 mg/kg, P.O) and sildenafil group (received sildenafil; 5 mg/kg, P.O). Rats were treated with vinpocetine, cilostazol or sildenafil daily for six days. Diclofenac was injected on days four, five and six in all groups, except control group. On the seventh day, animals were sacrificed. Vinpocetine, cilostazol and sildenafil significantly reduced diclofenac-induced elevation in the serum levels of urea, creatinine and cystatin C as well as renal tissue contents of TNF-α, NF-κB, TLR4 and HMGB1. This was reflected on the marked improvement in histopathological changes induced by diclofenac. This study revealed the good protective effect of these phosphodiesterase inhibitors against diclofenac-induced acute renal failure. Sildenafil showed the best protection regarding TNF-α and NF-κB, while cilostazol showed the best results regarding TLR4, HMGB1 and histopathological examination.
Biography Nermeen Salah El Deen is focused on improving public health and wellbeing. She started her career as a Demonstrator in the Pharmacology and Toxicology Department, Faculty of Pharmacy at Cairo University. Her work is based on decreasing acute renal failure rates caused by diclofenac which is one of the most commonly used NSAIDS through pre-treatment with different phosphodiesterase inhibitors.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 56 Berardo C et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
MPEP protection against hepatic ischemia/reperfusion injury is associated to TNF-α decrease Berardo C1, Di Pasqua LG1, Siciliano V1, Nicoletti F2,3, Plinio R1, Vairetti M1 and Ferrigno A1 1University of Pavia, Italy 2IRCCS Neuromed, Italy 3Sapienza University, Italy
he Negative Allosteric Modulator (NAM) 2-methyl-6-(phenylethynyl) pyridine (MPEP) of metabotropic Glutamate TReceptor subtype 5 (mGluR5) improves viability of isolated anoxic hepatocytes1. It is known that during ischemia/ reperfusion (I/R) injury, Kupffer cells activation occurs, causing an increase in TNF-α production. Moreover, TNF-α positively correlates with iNOS expression through NF-κB2,3 and because of the presence of TNF Response Element on iNOS gene4,5.The aims of this study were: to investigate the protection mediated by other NAMs (MTEP and Fenoabam) against anoxic damage in isolated hepatocytes and to investigate the liver functionality in two ex vivo models of I/R. Male Wistar rat hepatocytes were exposed to 90 minutes anoxia at 37°C with: MPEP and 3-((2-methyl-4-thiazolyl ethynyl pyridine (MTEP) at 3-30μM, Fenobam at 1-10-50-100μM. Hepatocytes viability was evaluated by trypan blue exclusion and LDH release. Wildtype and mGluR5 knockout livers from Balb-c mice were isolated, subjected to cold or normothermic I/R and treated with MPEP 0.3μM; transaminases release, BAX and Bcl-2, iNOS, eNOS and TNF-α protein expression were evaluated in cold I/R samples, while LDH, AST, TNF-α release were evaluated in normothermic samples. MPEP 30μM, MTEP 3μM and Fenobam 50μM improved significantly anoxic isolated hepatocytes viability respect to anoxic controls. MPEP addition during I/R significantly reduced LDH, transaminases and TNF-alpha respect to ischemic controls in both cold and normothermic I/R, with a trend similar to mGluR5 knockout samples. Furthermore, MPEP reduced TNF-α levels, causing selectively iNOS expression decrease without affecting eNOS expression. Our results demonstrated that MPEP, MTEP and Fenobam protect rat hepatocytes against ischemic injury. Furthermore, MPEP is able to reduce susceptibility of liver grafts to the preservation injury in two ex vivo models, in which the pharmacological blockade of mGluR5, interrupting the excitotoxic cascade, reduces the inflammation mediator TNF-α. Recent Publications 1. Storto et. 2000. Selective blockade of mGlu5 metabotropic glutamate receptors protects rat hepatocytes against hypoxic damage. Hepatology. 2. Nandi et al. 2010. TNF-α modulates iNOS expression in an experimental rat model of indomethacin-induced jejunoileitis. Molecular and Cellular Biochemistry, 336(1–2), pp.17–24 3. Fonseca et al. 2003. TNF-α mediates the induction of nitric oxide synthase in macrophages but not in neutrophils in experimental cutaneous leishmaniasis. European Journal of Immunology, 33(8), pp.2297–2306 4. Muntané et al. 2000. TNF-alpha dependent production of inducible nitric oxide is involved in PGE(1) protection against acute liver injury. Gut, 47(4), pp.553–62 5. Medeiros et al. 2007. Connecting TNF- α Signaling Pathways to iNOS Expression in a Mouse Model of Alzheimer’s Disease: Relevance for the Behavioral and Synaptic Deficits Induced by Amyloid Protein. Journal of Neuroscience, 27(20), pp.5394–5404.
Biography Clarissa Berardo, Doctorate in Biomedical Sciences, curriculum Pharmacology. Master Degree in Molecular Biology and Genetics. She has her expertise in mouse and rat liver isolation to study preservation techniques for organ transplantation (Cold Storage and Machine Perfusion), hepatocytes isolation from mouse and rat, primary and tumoral (HepG2 and Huh7.5) cell culture, use of genetic (Zucker) and nutritional (Methionine and Choline Deficient Diet) animal models to study hepatic diseases, such as NAFLD, NASH and ischemia/reperfusion injury. Knowledge of principal techniques performed in biomedical laboratory: western blot analysis, RT-PCR, histological techniques, biochemical and enzymatic assays.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 57 conferenceseries.com
JOINT EVENT 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain Scientific Tracks & Abstracts Day 2
Pharmacology 2018 & Chromatography-HPLC Congress 2018
Page 63 Sessions: Day 2 August 03, 2018 Chromatography-HPLC Separation Techniques | Chromatography-Mass Spectroscopy Analysis Major Chromatography Techniques | Method Development and Validation | Others
Session Chair Tomasz Tuzimski Medical University of Lublin, Poland
Session Introduction Title: High-performance liquid chromatography mass spectrometry of thiolate-protected metal clusters Yuichi Negishi, Tokyo University of Science, Japan Title: HPLC estimation of antioxidants via D-phenylalanine hydroxylation and tetrahydrobiopterin during oxidative stress Roberto Biondi, Alessandrescu Rusescu National Institute for the Health of Mother and Child, Romania Title: Role of chromatographic techniques for drug discovery and development Madhuri Singhal, Government M V M College, India Title: Isolation and identification of a natural diterpenoid from Gymnocoronis spilanthoides with trypanocidal activity Mariana Gabriela Selener, University of Buenos Aires (UBA), Argentina Title: Novel pyrazoline as a new reagent for quantifying primary alcohols using HPLC-FLD Amal Al Sabahi, Sultan Qaboos University, Oman Title: Retention behavior of structurally-diverse drugs on biopartitioning micellar chromatography and its potential to estimate cell permeability Panagiotis Danias, National Technical University of Athens, Greece Title: Optimization of extraction conditions (QuEChERS/d-SPE) and quantitative analysis of xenobiotics (pesticides, dyes, medicines, bisphenols) in food products and biological samples using high-performance liquid chromatography (HPLC) combined with modern detection techniques Tomasz Tuzimski, Medical University of Lublin, Poland
Pharmacology 2018 & Chromatography-HPLC Congress 2018
Page 64 Yuichi Negishi, Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
High-performance liquid chromatography mass spectrometry of thiolate-protected metal clusters Yuichi Negishi Tokyo University of Science, Japan
mall thiolate-protected gold clusters have attracted considerable attention as new functional nanomaterials because they Shave size-specific properties and functions that are not found for bulk gold. In particular, hydrophilic thiolate-protected gold clusters (hereinafter referred to as hydrophilic gold clusters) exhibit high biocompatibility and luminescence quantum yield in addition to pollution-free properties. Therefore, hydrophilic gold clusters are expected to be used in biomedical and environmental applications. Replacing some of the Au atoms in these clusters with different elements may impart them with even more useful functions. However, the synthesis of hydrophilic metal clusters has been less studied because of the complexity involved in evaluating the mass distributions of product mixtures. In this work, we found two hydrophilic interaction liquid chromatography (HILIC) columns for high-performance liquid chromatography (HPLC) suitable for the high-resolution separation of hydrophilic metal clusters. The mass distributions of the product mixtures of hydrophilic metal clusters were evaluated via HPLC mass spectrometry (LC/MS) using these HILIC columns. Consequently, we observed multiple clusters that
had not been previously reported for glutathionate (SG)-protected gold clusters (Aun (SG)m). Additionally, we demonstrated
that Aun−xMx(SG)m alloy clusters (M=Ag, Cu, or Pd) in which part of the Au in the Aun (SG)m cluster is replaced by a hetero element can be synthesized, similar to the case of hydrophobic alloy clusters. It is easy to evaluate the mass distributions of hydrophilic metal clusters using this method. Thus, remarkable progress in the synthesis techniques of hydrophilic metal clusters through the use of this method is anticipated, as is the situation for hydrophobic metal clusters.
Figure: Schematic representation of this work Recent Publications 1. Y Negishi, et al., (2018) High-performance liquid chromatography mass spectrometry of gold and alloy clusters protected by hydrophilic thiolates. Nanoscale 10:1641-1649. 2. Y Negishi, et al., (2016) Precise synthesis, functionalization and application of thiolate-protected gold clusters. Coordination Chemistry Reviews (320-321) 238-250. 3. Y Negishi, et al., (2016) High-resolution separation of thiolate-protected gold clusters by reversed-phase high-performance liquid chromatography phys. Chem. Chem. Phys. (Perspective), 18:4251-4265. 4. Y Negishi, et al., (2015) Understanding ligand-exchange reactions on thiolate-protected gold clusters by probing isomer distributions using reversed-phase high-performance liquid chromatography. ACS Nano, 9:9347-9356. 5. Y. Negishi, et al., (2015) A critical size for emergence of nonbulk electronic and geometric structures in dodecanethiolate- protected au clusters. J. Am. Chem. Soc., 137:1206-1212.
Biography
Yuichi Negishi has completed his PhD at Keio University, Japan. He is a Professor of Tokyo University of Science, Japan. He has over 140 publications that have been cited over 7,200 times. In his publications, 10 papers are categorized to top 1% cited papers. His publication H-index is 45. He has been awarded several prizes, including the PCCP Prize (2007), CSJ Award for Young Chemists (2008), Japan Society of Molecular Science Award for Young Chemists (2012), and Yagami Prize (2017).
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 65 Roberto Biondi, Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
HPLC estimation of antioxidants via D-phenylalanine hydroxylation and tetrahydrobiopterin during oxidative stress Roberto Biondi Alessandrescu Rusescu National Institute for the Health of Mother and Child, Romania
iving organisms have developed a complex antioxidant system to counteract reactive oxygen species (ROS) and oxidative Lstress (OS) damage. An array of small antioxidant molecules (vitamin C, vitamin E, and flavonoid) were part of the antioxidant barrier and were evaluated using a detection system based on Fenton reaction-mediated D-phenylalanine (D-Phe) hydroxylation. This reaction in turn generated o-tyrosine, m-tyrosine and p-tyrosine that were separated by HPLC (high performance liquid chromatography) with fluorescence detectors. The addition of antioxidants competed with D-Phe on .OH attack, thus allowing to determine the .OH scavenger capacity. Using a kinetic approach, the hydroxyl radical scavenger capacity was applied to biological fluid samples but also to antioxidants as N,N dimethylthiourea. In order to define the process of ROS- mediated tetrahydrobioptern (BH4) degradation in isolated rat hearts subjected to ischemia, a direct HPLC assay of BH4 and its pteridine derivatives was used. The lack of oxygen due to myocardial ischemia determined ROS generation and consequently caused a BH4 cardiac content decline while the dihydroxanthopterin was produced. Contrarily other researchers found a BH4 depletion with BH2 increased levels in the vessels of a range of cardiovascular disease including hypercholesterolemia, diabetes, atherosclerosis, hypertension and heart failure. HPLC estimation was based on indirect method in which BH2 and BH4 were oxidized to biopterin by iodine and then evaluated by fluorescence detector. It is debated whether the provision of OHSC (OH scavenging capacity) assay to the industries producing artificial human milk, types of tea and wine might better improve the knowledge of their antioxidant properties. Regarding to pterin biosynthetic pathway, it is discussed if the ischemia- and OS-determined BH4 alteration could result more comprehensive using a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method. This technique could be applied to amniotic fluid samples collected from pregnant women suffering from preeclampsia in whose pathogenesis the main role is due to ischemia/reperfusion- caused ROS generation.
Biography
Roberto Biondi completed his undergraduate degree in Medicine and Surgery from University of Perugia, Italy; pursued PhD in Biological Sciences from Pisa University, Pisa, Italy. He is currently a Visiting Professor at the Maternal-Fetal Assistance Excellence Unit, of Alessandru Rusescu National Institute for Health of Mother and Child, Bucharest, Romania. He has developed a method on the detection and scavenging of hydroxyl radical via D-phenylalanine hydroxylation in human fluids at the same institute. He has worked on the detection of tetrahydrobiopterin oxidation products in myocardial ischemia and also during chemical oxidation at both Johns Hopkins University in Baltimore, Maryland, USA and Ohio State University in Columbus, Ohio, USA.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 66 Madhuri Singhal, Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Role of chromatographic techniques for drug discovery and development Madhuri Singhal Government M V M College, India
hromatographic techniques are extensively used for isolation and separation of bioactive constituents present in Cdifferent plants having medicinal value. Plant extracts after cold and hot extraction process were subjected to column chromatography for the separation of different bands corresponding to class of compounds using different solvents in various proportions. Portions having sufficient compound, after evaporation are separated to a single compound by using thin layer chromatography. In her research work author has isolated novel isoflavone glycoside, prenylated flavone glycoside and novel triterpenoidal saponin from the plant Pithecellobium dulce. She has also isolated different compounds from plantsTerminalia bellerica, Moringa oleifera and other indigenous medicinal plants. The extract of Terminalia bellerica was found to possess anticancer activity while the extract of plant Moringa oleifera was found to have anti-diabetic activity. The application of chromatography technique on natural products is quite useful in order to form herbal based drug with no side effects and toxicity.
Pic1: Pithecellobium dulce Pic2: Terrminalia belerica Recent Publications 1. Anjali Jijhotiya, Madhuri and Sadhna Goyal (2018) Qualitative and Quantitative phytochemical estimation of leaves extract of plant Plumbago Zeylancia. International Journal of recent Scientific research 91(F):23249-23252. 2. Anjali Jijhotiya, Madhuri Singhal and Sadhna Goya (2016) Preliminary Phytochemical Screening of Leaves Extracts of Nicotiana Tabacum. International Journal of Engineering Technology Science and Research 4(6). 3. Asma Sultan and Madhuri Singhal (2017) Comparative antibacterial activity of tropical medicinal plants Aegle Marmelos, Phyllanthus Niruri and Aloe Barbedens against gram positive and gram negative bacteria. Journal of Education and Applied Science Research 4(3):30-33. 4. Packia Lakshmi (2017) Phytochemical Screening and Antimicrobial activity of moringa oleifera and mass spectra. International Journal of Engineering Technology Science and Research 4(8):156-161. 5. Madhuri Singhal, Rishikant Asati, Anjali Jijhotiya and Mohini Saxena (2016) Studies on some trace element accumulation in terminalia bellerica growing on Cu and Mn mine waste dump. International Journal of Research 3(10):436-441.
Biography
Madhuri Singhal has completed her PhD at Dr Hari Singh Gaur University, Sagar and Postdoctoral work at Allahabad University, Allahabad. Her research area is the role of natural products from medicinal plants in drug discovery and development. She has presented research papers in international conferences in Australia in 2005 and was invited as visiting academic in 2006 at Australian University. She has presented papers in USA in 2009 and 2015. In 2010, she has presented research paper at Ubon Ratchathani University, Thailand. In 2011, she has presented paper in Hong Kong. She is an Editorial Board Member of an international research journal. More than 30 research papers have been published. At present, she is a Professor of Chemistry in Government Motilal Vigyan Mahavidyalaya, Bhopal.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 67 Mariana G Selener et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Isolation and identification of a natural diterpenoid from Gymnocoronis spilanthoides with trypanocidal activity Mariana G Selener1, Augusto Bivona1, Natacha Cerny1, Andrés Sanchez Alberti1, Emilio Malchiodi1, Flavia Redko1, Valeria P. Sülsen1 and Cesar Catalan2 1University of Buenos Aires, Argentina 2National University of Tucuman, Argentina
hagas disease is a parasitic disease caused by the protozoan Trypanosoma cruzi. According to the World Health Organization C(WHO) this parasitosis affects 6-7 million people worldwide. In Argentina it is estimated that approximately 1.5 million people are infected. The current available drugs used for its treatment, nifurtimox and benznidazole, have limitations due to host toxicity, side effects and low efficacy. In this context, it is extremely necessary to develop new drugs. Nature has provided useful drugs that are used nowadays to treat different pains. Asteraceae species have been a rich source of active compounds and have been attractive for drug discovery. In previous work the trypanocidal activity of the dichloromethane extract of Gymnocoronis spilanthoides (Asteraceae) [GSDE] has been demonstrated. The aim of this investigation was to isolate and identified the active compounds present in GSDE. GSDE was purified by liquid-liquid partition and fractionated by column chromatography using
Silicagel-60 and a gradient of CH2Cl2 and EtoAc. From fractions eluted with CH2Cl2: EtoAc (2:1) a pure compound was isolated (compound A). The GSDE as well as compound A were analyzed by HPLC (C18 column, linear gradient elution mode and UV/Vis absorbance detector). The structure elucidation of the isolated compound was performed by spectroscopic methods. The trypanocidal activity of compound A was evaluated on T. cruzi epimastigotes (RA) by the [3H]-thymidine uptake assay. The cytotoxicity of this compound on mammalian cells was performed using mouse splenocytes. Compound A presented a
significant trypanocidal activity (IC50= 1.6 µg/ml). This compound showed some toxicity to mammalian cells (CC50=4.9 µg/ ml). The compound A presented 98% purity (by HPLC) and was identified as the ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid. The trypanocidal activity of ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid on trypomastigote and amastigote forms will be evaluated. We will also continue with the isolation and identification of other compounds present in the active extract.
Figure: HPLC chromatographic analysis (*) and chemical structure of compound A (*): Luna® 5 µm C18 (2) 100 Å, LC Column 250 x 4.6 mm. Gradient Water: ACN. PDA: 240 nm. Flow: 1.0 ml/min. Injection volume: 20 µl. Recent Publications 1. World Health Organization (2018) Fact sheet Chagas disease (American trypanosomiasis). http://www.who.int/mediacentre/ factsheets/fs340/en/. Accessed April 2018. 2. Sülsen V, et al. (2013) Natural terpenoids from Ambrosia species are active in vitro and in vivo against human pathogenic trypanosomatids. PLoSNegl Trop Dis. 7(10):e2494. 3. Herz W and Sharma R (1976) New hydroxylatedent-kauranoic acids from Eupatorium album. J. Org. Chem., 41(6):1021-1026.
Biography
Mariana G Selener has completed her Bachelor degree in Pharmacy in the Faculty of Pharmacy and Biochemistry at University of Buenos Aires (UBA) in 2011. She is pursuing her PhD at Chair of Pharmacognosy at the same University. She has expertise in analytical development. The aim of her PhD research work is the isolation and identification of bioactive compounds from Argentine Asteraceae species. She is an Assistant at the Chair of Pharmacognosy at the Faculty of Pharmacy and Biochemistry, University of Buenos Aires (UBA) for undergraduate courses.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 68 Amal Al Sabahi, Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Novel pyrazoline as a new reagent for quantifying primary alcohols using HPLC-FLD Amal Al Sabahi Sultan Qaboos University, Oman
Statement of the Problem: Many labeling reagents are commercially available for quantifying primary alcohols; however, these reagents show some drawbacks, such as toxicity, lack of sensitivity and selectivity, low solubility, and high cost. Using fluorescent heterocyclic compounds as labels is an outgrowing field in analytical chemistry. Pyrazolines are well known heterocycle owning fascinating photophysical properties that qualify them for sensing and imaging of bioorganic molecules. The applications of these dyes span many areas from photodynamic cancer therapy, organic light emitting diodes, to fibers whitening and brightening. However, recently it has been evaluated as potential candidates for pre-column derivatization of amino acids and neurotransmitters. The purpose of this study is to evaluate the adequacy of the newly synthesized pyrazoline, 4-(1-(4-trifluoromethyl) phenyl)-4, 5-dihydro-3-(naphthyl)-1H-pyrazole-5-yl) benzoic acid (TFNPB) as a label for primary alcohols and to develop a pre-column derivatization method for quantifying these analytes in different matrices. Methodology & Theoretical Orientation: TFNPB was synthesized by the conventional method, which involves two steps, an aldol condensation reaction between acetyl-naphthalene and 4-formylbenzoic acid followed by Michael addition of the phenyl-hydrazine. The photophysical properties including absorption, emission, and lifetime measurements have been studied in different solvents. Primary alcohols were then derivatized by this reagent, and LC-MS was used to assess the produced derivatives. The derivatization procedure was optimized, and the assay of alcohols by this method was validated. Findings: TFNPB shows excellent photophysical properties including high fluorescence intensity and quantum yield. It emits in the visible region at 460 nm in acetonitrile. It is used to derivative hydroxyl groups instantly at low temperature and in short reaction time. Alcohol derivatives show strong well-separated peaks (resolution µ1.5) on C8 column using 75% ACN in water. The produced derivatives were stable at room temperature for more than one month. Excellent linear relationships were obtained for four alcohols in the range 1.25-94 μmol L-1 (R2≥0.991). All figures of merit were calculated and the method developed was validated for the quantitative analysis of primary alcohols.
Fig 1: schematic diagram of TFNPB Fig 2: Chromatogram of alcohols derivatives. Recent Publications 1. You J, Sun X, Lao W and Ou Q (1999) Determination of alcohols using condensation agent carbazole-9-acetyl-benzene- disulfonate by high performance liquid chromatography with pre-column fluorescence derivatization. Chromatographia 49(11-12):657-665. 2. Yoshikawa M and Tani C (2003) Sensitive determination of alkoxyethanols by pre-column derivatization with 1-anthroylnitrile and reversed-phase high-performance liquid chromatography. Journal of Chromatography A 1005(1-2):215-221. 3. Havrylyuk D, Zimenkovsky B, Vasylenko O, Zaprutko L, Gzella A and Lesyk R (2009) Synthesis of novel thiazolone-based compounds containing pyrazoline moiety and evaluation of their anticancer activity. European Journal of Medicinal Chemistry 44(4):1396-1404.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 69 conferenceseries.com JOINT EVENT 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
4. Lu Z, Jiang Q, Zhu W, Xie M, Hou Y, Chen X, Wang Z, Zou D and Tsutsui T (2000) Efficient blue emission from pyrazoline organic light emitting diodes. Synthetic Metals 111:425-427. 5. Varghese B, Al-Busafi S N, Suliman F O and Al-Kindy S M (2016) 3-Naphthyl-1-phenyl-5-(4-carboxyphenyl)-2-pyrazoline–a pyrazoline based heterocyclic dye as a fluorescent label for biomolecules containing an amino group and its evaluation using HPLC. Analytical Methods 8(13):2729-2736.
Biography
Amal Al Sabahi has completed her BSc in Science Education at Sultan Qaboos University (SQU) in 1996 and MSc in Chemistry in Department of Chemistry, College of Science at SQU in 2003. Currently, she is pursuing PhD in Chemistry in the same department. She worked as a Chemistry Teacher for 10 years and as Educational Researcher for six years. She worked as a Lab Instructor in SQU for three years.
Notes:
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 70 Fotios Tsopelas et al., Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Retention behavior of structurally-diverse drugs on biopartitioning micellar chromatography and its potential to estimate cell permeability Fotios Tsopelas1, P Danias1, C Stergiopoulos1, A Pappa1 and A Tsantili-Kakoulidou2 1National Technical University of Athens, Greece 2University of Athens, Greece
iopartitioning micellar chromatography (BMC) utilizes micelles formed by a surfactant, such as polyoxyethylene (23) Blauryl ether (Brij35), in a concentration higher than its critical micelle concentration and a reversed-phase stationary phase in order to gain insight into drug-membrane interactions, by rapid, friendly and reproducible measurements. The chromatographic column, modified by the surfactant, resembles the ordered array of the membranous hydrocarbon chains in regards to hydrophilic/hydrophobic character and the interactions of xenobiotics with the H-bonding groups of the adsorbed surfactant similar to the membrane ones. Therefore, the characteristics of the BMC are similar to biological barriers. Up to now, the reported studies of BMC to model toxicity (LD50), blood-brain barrier penetration, plasma clearance, volume of distribution as well as oral absorption are based on limited datasets and therefore, further investigations are needed. The aim of the present study was the evaluation of the potential of biopartitioning micellar chromatography to estimate cell permeability. For this purpose, retention indices (logkw) of an extended set of structurally-diverse drugs were measured on a discovery RP- 18 column using as eluent phosphate buffer in the presence of Brij at a concentration of 0.04 M. The effect of the addition of NaCl in a concentration of 9.2 g/L was studied as well as the effect of increase of temperature from ambient to 37oC. Retention factors were compared with octanol-water partitioning and retention factors obtained in immobilized artificial membrane (IAM) chromatography. Retention factors were subsequently compared with Madin Darby Canine Kidney (MDCK) cell lines permeability data taken from literature and they were used to model % Human Oral Absorption (% HOA) data, compiled from literature sources. For reasons of comparison, the constructed models were compared with those derived by octanol-water partitioning.
Recent Publications 1. Vucicevic J, Popovic M, Nikolic K, Filipic S, Obradovic D and Agbaba D (2017) Use of biopartitioning micellar chromatography and RP-HPLC for the determination of blood-brain barrier penetration of a- adrenergic/ imidazoline receptor ligands and QSPR analysis. SAR and QSAR in Environmental Research 28:235-252. 2. Stepnik KE, Malinowska I and Roj E (2014) In vitro and in silico determination of oral, jejunum and Caco-2 human absorption of fatty acids and polyphenols. Micellar liquid chromatography. Talanta 130:265-273. 3. Hadjmohammadi M and Salary M (2013) Biopartitioning micellar chromatography with sodium dodecyl sulfate as a pseudo α1-acid glycoprotein to the prediction of protein- drug binding. Journal of Chromatography B 912:50-55.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 71 conferenceseries.com JOINT EVENT 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
4. Tsopelas F, Giaginis C and Tsantili- Kakoulidou A (2017) Lipophilicity and biomimetic properties to support drug discovery. Expert Opinion on Drug Discovery 12:885-896. 5. Stepnik K E and Malinowska I (2013) The use of biopartitioning micellar chromatography and immobilized artificial membrane column for in silico and in vitro determination of blood- brain barrier penetration of phenols. Journal of Chromatography A 1286:127-136.
Biography
Fotios Tsopelas is a Lecturer in the School of Chemical Engineering at National Technical University (NTUA), Greece. He studied Chemical Engineering in the NTUA (1999) and Pharmacy (2004) at National and Kapodistrian University of Athens. He completed his PhD in 2007 in Environmental Analytical Chemistry at NTUA (scholarship from Onassis Foundation) and his Postdoctoral research was focused on biomimetic chromatography for novel drug design. He has more than 25 publications in peer-reviewed scientific journals and more than 40 contributions in international conferences. He has acted as a Referee in more than 20 international journals. He has participated as a member of scientific committee in two international conferences. He has coordinated five national and international funded research projects. His research interest is mainly focused on the development of biomimetic chromatographic approaches for the evaluation of pharmacokinetic properties of candidate drugs and ecotoxicity of emerging pollutants.
Notes: Supported by the Onassis Foundation under the ‘‘Special Grant and Support Program for Scholars’ Association Members” (Grant No. R ZN 004-1/2017-2018).
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 72 Tomasz Tuzimski, Biochem Pharmacol (Los Angel) 2018, Volume 7 conferenceseries.com JOINT EVENT DOI: 10.4172/2167-0501-C1-009 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
Optimization of extraction conditions (QuEChERS/d-SPE) and quantitative analysis of xenobiotics (pesticides, dyes, medicines and bisphenols) in food products and biological samples using high- performance liquid chromatography combined with modern detection techniques Tomasz Tuzimski Medical University of Lublin, Poland
isphenols are a class of chemicals with two hydroxyphenyl functionalities, which include bisphenol A (BPA) and several Banalogues such as bisphenol S (BPS), bisphenol F (BPF), and bisphenol B (BPB). As industrial chemical, BPA is widely used in the production of polycarbonate plastics (used in food contact materials, such as food containers, baby food and water bottles), epoxy resins (used as internal coating in canned food and beverage to prevent the food to get in contact with the metal wall and the deterioration of cans) and as an antioxidant in polyvinyl chloride (PVC) plastics in materials intended to come into contact with food (packaging cling films). Considering the similarity in the structure of various analogues can migrate from food containers. Due to their fat content, both breast and dietary milk could be polluted by many xenobiotics characterized by lipophilic properties. Breast milk, milk of lactating cows and dairy products are widely consumed by infants, children and many adults throughout the world and occurrence of quantifiable amounts of BPA represents a matter of public health concern. Due to the ability to accumulate in organisms and in the food chain, BPA may also affect the development of subsequent generations. The breastfeeding could represent a source of exposure to infants, because BPA tends to persist for extended periods of time and infants can be more susceptible to adverse effects resulting from chemical exposures, due to the rapid mental and physical changes that take place during prenatal and neonatal periods. Despite the reported levels in food are generally considered safe, the occurrence of BPA residues in breast milk and dairy products should be considered significant in terms of potential human-health risk. The aim of the paper was determination of xenobiotics (bisphenols, pesticides, dyes, medicines) in breast milk samples and food products.
Fig: MRM Chromatogram showing separation of the bisphenols standards by LC-QqQ-MS/MS Recent Publications 1. Mercogliano R and Santonicola S (2018) Investigation on bisphenol A levels in human milk and dairy supply chain: a review. Food and Chem Toxicol. 114: 98–107. 2. Arcaro K F, et al. (2014) Determination of free bisphenol A (BPA) concentrations in breast milk of US women using a sensitive LC/MS/MS method. Chemosphere 104: 237–243. 3. Rejczak T and Tuzimski T (2017) Application of high-performance liquid chromatography with diode array detector for simultaneous determination of 11 synthetic dyes in selected beverages and foodstuffs. Food Analytical Methods 10(11):3572–3588.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 73 conferenceseries.com JOINT EVENT 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques August 02-03, 2018 | Barcelona, Spain
4. Rejczak T and Tuzimski T (2017) method development for sulfonylurea herbicides analysis in rapeseed oil samples by HPLC–dad: comparison of zirconium-based sorbents and EMR-lipid for clean-up of QuECHERS extract. Food Analytical Methods 10(11):3666–3679.
Biography
Tomasz Tuzimski is an Adjunct Professor in Department of Physical Chemistry at Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, Poland. He has completed MA degree and PhD degree in 1995 and 2002, respectively, and Doctor of Sciences in Habilitation in 2012 at the Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin. His scientific interests include the theory and application of liquid chromatography, extraction (QuEChERS) and detection techniques. He has authored or co-authored more than 62 scientific papers (IF=100, H-index=17). He is a member of the Editorial Board of the Acta Chromatographica. He is Quest Editor of special sections on pesticide residues analysis in the JAOAC Int. He has co-authored and co- edited with Professor Joseph Sherma the book entitled “High Performance Liquid Chromatography in Pesticide Residue Analysis” and the book “Determination of Target Xenobiotics and Unknown Compounds Residue in Food, Environmental and Biological Samples”.
Biochemistry & Pharmacology: Open Access Volume 7 ISSN: 2167-0501 Pharmacology 2018 & Chromatography-HPLC Congress 2018 August 02-03, 2018 Page 74