1 MLTN 230 Immunology/Immunohematology

MLTN 230 Immunology/Immunohematology Lecture CRN 28365 (3-0-3)

Semester: Spring 2019 Instructor: Diane Tyson, MS, MLS(ASCP), CLS(CA) Phone number: (304) 929-5038 E-mail address: [email protected] Class Hours and Location Lecture: Wed, 9 – 11:50 am, R135 Lab: Wed, 1 – 4:50 pm, Room RAH17, Allied Health Wing Office Location: R137, Raleigh County Campus Office Hours: Mon – by appt Tues – 11 am – 4 pm Thurs – 11 am to 4pm Fri – by appt Course Deliver Method: TRAD Help Desk: 304-929-6725

New River Vision Statement: New River Community and Technical College improves students’ lives and enriches communities.

New River Mission Statement: New River Community and Technical College provides accessible, affordable, quality education and workforce programs to meet the needs of the region it serves.

New River Social Justice Statement: Consistent with its comprehensive mission and recognizing that the development of human potential is a fundamental goal in a democratic society, the New River Community and Technical College Board of Governors promotes an educational system that values cultural and ethnic diversity and understanding; that provides for the preparation of students for full and meaningful participation in a changing world; and that promotes equitable and fair treatment in every aspect of campus life and employment for all persons regardless of race, national origin, gender, sexual preference, sexual orientation, age, religion, veteran status or disability.

Course Description: This course is intended to prepare you with the knowledge, theory and skills to perform immunology, serology and immunohematology (transfusion and blood bank) analysis. You will also be trained in other important skills such as microscopy, ensuring patient safety, specimen collection and processing, quality control and assurance, critical thinking skills, communication skills and professional behavior. YOU MUST EARN A MINIMUM 75% IN BOTH

THE LECTURE AND LABORATORY TO PASS THIS COURSE AND PROCEED TO THE NEXT SEMESTER! You are allowed to retake the course (must take entire course) one time only if failing any portion of the course, lecture or lab. If you fail either portion of the course a second time, you will be dismissed from the program.

Primary Textbook(s): Immunology & Serology in Laboratory Medicine, 6th ed. 2018, author Mary L. Turgeon. Publisher Mosby Elsevier. ISBN: 9780323431477 Basic & Applied Concepts of Blood Banking & Transfusion Practices, 4th ed, 2017, author, Paula Howard. Publisher Mosby Elsevier. ISBN: 9780323374781 Lab Manual: Provided by Instructor and/or posted on Blackboard. Publisher Online Student Resources: Evolve by Elsevier: www.evolve.elsevier.com Student resources our available on Blackboard, including power points, images and videos. University of Washington MedTraining: www.medtraining.org This is an online training and competency program. You will be required to complete these assignments continuously throughout the program. MediaLab: www.medialab.inc This is another powerful online training and competency program. You will be required to complete these assignments continuously throughout the program. MediaLab under Exam Review has exam reviews, practice exams and case studies that are accessible for all students throughout the MLT program.

Outline IMMUNOLOGY AND IMMUNOHEMATOLOGY (3-0-3)

Immunology Portion: (first 6 weeks) This is an introductory course in immunology designed for undergraduate medical laboratory technicians and medical laboratory science students. Lecture topics include:  Antigens and antibodies  Cells and cellular activities of the immune system  Soluble mediators of immunity  Quality Assurance & Quality Control  Labeling Techniques in Immunoassay  Basic Molecular Techniques Fundamentals The laboratory component of the course stresses a variety of methods, including:  Basic Serological Laboratory Techniques  Direct agglutination  Precipitation  Passive agglutination  Immunodiffusion / gel diffusion  Enzyme immunoassays  Electrophoresis 2

 Molecular techniques In addition to the basic principles of laboratory procedures, the course will address technical sources of error, procedural limitations, and clinical applications. Selected classic case studies will be presented to demonstrate the role of laboratory testing in the diagnosis and treatment of diseases. The overall goals of this course are:  To introduce the student to the basic fundamentals of immunology and to apply this knowledge to selected clinical examples  To familiarize the student with the underlying principles of serologic testing  To introduce the student to the basic concepts of the immunologic response to infectious disease.

COURSE OUTLINE: 1. Basic Immunologic Mechanisms a. Overview of Immunology b. Antigens and antibodies c. Cellular activity of the immune system - Granulocytes and Mononuclear cells - Lymphocytes and Plasma cells d. Soluble Mediators of the Immune System - Complement and the pathways - Cytokines, interleukins, interferons and other hematopoietic stimulators

2. Theory of Immunologic and Serologic Procedures a. Quality assurance and quality control b. Basic serologic techniques (dilutions) c. Point-of-Care Testing d. Agglutination methods e. Electrophoresis techniques, including Immunofixation electrophoresis (IFE) f. Labeling Techniques in Immunoassays g. Molecular techniques, including PCR, HIV-1 immunoblotting

3. Immunologic Manifestations of Infectious Diseases a. Viral disease - EBV / Infectious mononucleosis - Hepatitis - HIV / HTLV / CMV - Rubella / measles - Other viruses b. Microbial - Streptococcal infections - Cold agglutinins - Syphilis - Other microorganisms 3

c. Vector-borne diseases

4. Immunologically and Serologically Related Disorders a. Hypersensitivity Reactions b. Autoimmune disorders - ANA, anti-DNA, ENA - Systemic Lupus Erythematosus - Rheumatoid Arthritis c. Transplantation (Bone marrow and organ)

Immunohematology Portion (last 8 weeks) This course in immunohematology (blood bank or transfusion services) is designed for undergraduate medical laboratory technicians and medical laboratory science students. Lecture topics include:  Blood group antigen and antibody reactions  Laboratory reagents for immunohematology  Major blood group systems  Essentials of pretransfusion testing  Clinical considerations The laboratory portion of the course stresses  Proper patient identification  Principles of various testing in the blood bank  Proper performance of testing in the blood bank  Proper selection of blood units for patient transfusion In addition to the basic principles of laboratory procedures, the course will address technical sources of error, procedural limitations, and clinical applications. Selected classic case studies will be presented to demonstrate the role of laboratory testing in the clinical blood bank. The overall goals of this course are:  To introduce the student to the basic fundamentals of immunohematology and to apply this knowledge to selected clinical examples  To familiarize the student with the underlying principles of transfusion services testing

COURSE OUTLINE 5. Basic Sciences and Reagents a. Basic principles and applications b. Blood banking reagents c. Genetic principles

6. Major Blood Groups a. ABO b. Rh 4

b. Lewis c. P d. Ii e. Duffy f. Kell g. Kidd h. MNS

7. Essentials of Pretransfusion Testing a. Antibody screening and identification b. Multiple antibodies c. Compatibility testing d. Special tests - DAT - Phenotyping and genotyping - Elution / absorption - Antibody titer - Pre-warm technique - Rosette

8. Blood Collection, Testing and Components a. Donor selection and requirements b. Blood Component Preparation c. Transfusion Therapy - RBC - Platelets - Fresh frozen plasma - Cryoprecipitated Anti-hemophilic factor - RhIg

9. Clinical considerations in Immunohematology a. Adverse complications of transfusions b. Hemolytic Disease of the Fetus and Newborn c. Transfusion Therapy in Selected Patients

10. Quality Assurance, Quality Control and Regulation of the Blood Industry

Affective Outcomes: During the lecture, laboratory, clinical practicum, and upon completion of the program of study in Medical Laboratory Technology, the student will be able to demonstrate appropriate professional behavior, reliability and attitude skills. 1. Demonstrate professionalism by a. complying with the attendance policy, 5

b. complying with the dress code, c. submitting assignments by the stated deadline. 2. Demonstrate enthusiasm and interest in the profession by asking questions, participating in class discussions and meet with instructors during office hours as needed. 3. Demonstrate initiative by reviewing objectives and completing reading assignments prior to class. 4. Demonstrate progression in laboratory skills by effective organization, coordination of multiple tasks and insightful evaluation of results obtained. 5. Utilize constructive criticism to correct deficiencies and improve performance. 6. Work cooperatively with instructors and fellow students to achieve the goals of each activity. 7. Participate in activities designed to advance the profession of MLT and build professional pride.

Lecture Cognitive /Psychomotor Outcomes:

Upon completion of this course the student will be able to: 1. Describe the various quality assurance procedures and their uses that aid in the successful running of an Immunology and Immunohematology department. 2. Perform the basic tests that are performed in the Immunology and Immunohematology departments, such as syphilis and mono tests, blood typing, antibody screen and identification, and crossmatch procedures. 3. Explain / describe the analytical principles, specimen requirements and clinical significance of abnormal results of the various test performed in the immunology and immunohematology departments. 4. Explain the different aspects of the immune system and the function of each of the components. 5. Describe and explain aspects of common infectious diseases and other immunological disorders such as autoimmune diseases. 6. Utilize interpretive, analytical, critical thinking and problem solving skills in various immunology and immunohematology situations. 7. Evaluate and interpret laboratory assay data, evaluate procedures for sources of error, and resolve situational and QC problems, etc. 8. Define the term blood group system with regard to serologic and genetic classifications. 9. Calculate the probability of finding compatible red blood cell units for a recipient with multiple antibodies. 10. Discuss advantages and disadvantages of autologous and allogeneic donation and transfusion. 11. Review donor information and determine if donor is suitable, including the deferral criteria for infectious disease. 12. Describe the processes for separating units of whole blood and preparing the various components. 6

13. Discuss the clinical necessity and effects of transfusion and the medical purpose for component therapies. 14. Perform required preliminary investigation to determine whether a hemolytic reaction has occurred, and additional testing for suspect alloantibody. 15. List the biochemical changes that take place in stored units and relate to the specific anticoagulant used, time and temperature of storage. 16. Describe the immune process which causes HDFN and perform fetal blood testing. 17. Develop professionalism, communication skills, and interpersonal relationships, as evidenced in the Personal Professional Evaluation.

General Education Philosophy and Student Learning Competencies and Outcomes

General Education Philosophy. The purpose of general education at New River is to ensure that every student graduating with an associate degree, regardless of major or career objective, receives an exposure to the liberal arts, develops basic skills competency, and acquires a body of knowledge fundamental to that of an educated person. General Education Competencies and Outcomes. Students graduating from New River with an associate degree will demonstrate the following competencies: Critical Thinking/Problem Solving. This area of learning is defined as a habit of mind characterized by the comprehensive exploration and analysis of issues, ideas, artifacts, and events before accepting or formulating an opinion or conclusion.

 Apply critical thinking skills to a variety of subjects  Identify and implement strategies to solve problems  Demonstrate the ability to separate fact from opinion  Access information to answer questions, form opinions, or solve problems  Evaluate one’s own and others’ assumptions, beliefs, and opinions  Synthesize and analyze in-depth information from a variety of sources  State logical conclusions based on information obtained or provided

Communication. This area of learning incorporates the skill of exchanging information and meaning between or among individuals through a common system of symbols, signs and behaviors. This includes the communication process of conversing, speaking, listening, corresponding, and writing.

 Demonstrate a thorough understanding of context, audience, and purpose  Use appropriate, relevant, and compelling content to illustrate mastery of the subject

 Demonstrate detailed attention to and successful execution of a wide range of conventions particular to a specific discipline including organization, content, presentation, formatting, and stylistic choices  Locate and use a variety of supporting materials (explanations, examples, illustrations, statistics, quotations) responsibly by making appropriate references to support the presentation or establish the presenter’s credibility/authority on the topic  Use language choices that are imaginative, memorable, and compelling, and enhance the effectiveness of the communication  Use appropriate delivery techniques to deliver the compelling message, ensuring that the communicator appears polished and confident  Practice attentive and active listening, maintain solid eye contact, often paraphrasing the message of the speaker to ensure understanding, and accurately restate the opinions of others even when he or she disagrees

Cultural and Social Awareness. This area of learning is defined as the ability to see oneself as a member of the world community, knowing that one’s future is shared with others. Cultural and social awareness goes beyond mere exposure to cultural differences but requires the capacity to meaningfully engage with others, place social justice in historical and political context, and put culture at the core of transformative learning.

 Demonstrate an understanding of one’s own culture and the complexity of elements important to members of other cultures in relation to the history, values, politics, communication styles, economy, or beliefs and practices of that culture  Demonstrate evidence of adjustment in one’s own attitude and belief because of working within and learning from diversity of communities and cultures  Document evidence of experience in civic engagement activities and describe the learning as it relates to a reinforced and clarified sense of civic identity and continued commitment to public action  Apply ethical perspectives to a question, and accurately consider full implications of the application  Participate in teamwork activities helping the team move forward in relation to the team goals  Identify physical and psychological facets of personal wellness, including successful techniques for stress management

Procedures for Student Assessment: The purpose of assessment for continuous improvement is to ensure that teaching methods are appropriate to facilitating your understanding of the subject matter and mastery of its application. A variety of assessments will be used throughout the course to allow the Instructor to determine whether you are individually and collectively developing a full understanding of key

concepts, theories, and ideas and their application. These assessments will allow the Instructor to modify presentation of the material accordingly. In this course, the student will be evaluated by participation in the class (this also means attendance and punctuality), immediate response questions, adherence to safety procedures, professional performance evaluation, homework assignments, online assignments, quizzes, exams, and final exam.

LECTURE: Weighted Assessment of Student Learning: Exams 40% Homework / Online assignments 20% Immunology Final 20% Blood Bank Final 20%

LAB: Weighted Assessment of Student Learning: Immunology Practical 20% Blood Bank Practical 20% Case Studies 20% Lab Sessions 40%

Lab sessions are made up of the following weights per lab:

10% 40% 20% 10% 20% Timely Completion Accuracy of Lab Lab Manual & Report Lab Clean-up and Summary of theory of Lab Exercise Results Organization Safety Readiness & procedure

Grading Scale: A = 92 - 100% B = 83 - 91% C = 75 - 82% F = below 75%

Attendance: Students are expected to attend class. This is not a regular course but is a job-training course; therefore, you will be expected to have the same conduct and attendance and punctuality as any job. More than 2 complete absences (lecture and lab) may result in you being dropped from the course at the instructor’s discretion. In addition, if you miss laboratory sessions, you will not earn points for that particular lab. Repeated tardiness and leaving early will result in deduction of points with increasing number of points deducted with each additional occurrence. You must contact your instructor before class if you cannot make it to class or expect to be tardy. If you are running late on the day of class, I expect a voice mail on my office phone before 8:30. My number is 304-929-5038. Lab Practicals and Competencies are time consuming to complete and document. Please make every effort to attend and participate. Practicals cannot be rescheduled unless there is a catastrophic event. Refer to the New River CTC Student Handbook for the policy on attendance. 9

Homework You will have various homework assignments throughout the semester, including the training and competency assignments through MedTraining and MediaLab. All assignments are expected to be complete on-time, which is BEFORE the start of the next class day unless specified by instructor. No extensions will be given since you have a week to complete. You will have 2 opportunities to complete the MediaLab and MedTraining courses. The average of the 2 scores will be your grade for that course.

Exams/Quizzes Class quizzes and exams will consist of a variety of types of questions. There will be multiple-choice, matching questions and fill in. These tests will emphasize the lecture material, but you are also responsible for the material covered in the assigned textbooks, even if it is not discussed in class. It will be impossible to cover all the subject matter in the text, so you must learn some of that material on your own. Only one (1) class quiz can be made up and rescheduled if the student notifies the instructor BEFORE the class starting time. If you miss a second quiz, a score of 0 will count in your total. Quizzes are given on a weekly basis and cannot be made up or rescheduled. These quizzes and tests will emphasize the lecture material, but you are also responsible for the material covered in laboratory and the assigned textbook material, even if it is not discussed in class. Material from any portion of the course will be considered as possible subject matter for exams and quizzes.

Extra Credit Under no circumstances will extra credit be given.

Attire and Hygiene Scrubs are the best clothing to wear in class and lab since we will be performing invasive procedures and handling biohazard specimens. Clothing other than scrubs are acceptable (only at New River) if clean and modest. Scrubs and other clothing must be clean and in good condition and not revealing. Closed-toe and closed-heel shoes must always be worn at New River and during clinicals. Socks or hose must also be worn. Gloves must always be worn at all time when performing labs or specimen handling and changed between patients. Washing hands must be done every time gloves are removed. No dangling jewelry or excessive jewelry allowed (2 piercings per ear and ears only) or any facial piercings. No fake nails, or fingernail tip lengths longer than 1/8 inch. Nails must be clean. Hair must be clean, of a natural color, neatly styled and pulled back if longer than shoulder length. If tattoos are visible, these must be covered with clothing, bandaids or cosmetics. Proper hygiene is required at all time, including use of deodorants and other proper daily hygiene regiments. No use of perfume is allowed in class or during clinicals.

Class Policies 10

Disclaimer The schedule and procedures contained in this course syllabus are subject to change in the event of extenuating circumstances. The syllabus binds both the student and the instructor to the procedures outlined herein. In the event of a conflict between the contained procedures and those established policies of New River CTC, the college policies shall prevail.

Academic Integrity You are expected to adhere to the highest standards of academic integrity. Cheating in any form will not be tolerated. If in the instructor’s opinion the student has cheated, 0 points will be assigned for that activity. In addition, the Academic Dishonesty Report Form will be submitted to the VP of Student Affairs. Therefore, it is incumbent on the student to be certain that the instructor has no reason to doubt the student’s integrity in the activity. Other possible penalties will be at the instructor’s discretion, including receiving a letter grade of F for the course. Likewise, if they are caught plagiarizing someone else’s work, they will receive 0 points for that assignment. Additionally, the most severe penalties possible at this college will be actively pursued which may include one or more of the following: a score of zero or “F” on the assignment or exam; a reduced grade in the course, an “F” in the course; removal from class for one or two class days as outlined in the Education Code (section 76032) and referred to in the “Removal by Instructor” section of the Student Code of Conduct.

Academic dishonesty is defined as an act of deception in which a student claims credit for the work or effort of another person or uses unauthorized materials or fabricated information in any academic work. Academic dishonesty is a violation of the DVC “Student Code of Conduct” and will not be tolerated. Academic dishonesty diminishes the quality of scholarship at New River CTC and hurts the majority of students who conduct themselves honestly as well as harming those who are actively involved in the practice – it adversely affects everyone.

Acts of academic dishonesty include, but are not limited to the following: Cheating – Unauthorized copying or collaboration on a test or assignment, or the use or attempted use of unauthorized materials such as notes, visual signals, and electronic communications. Tampering – Altering or interfering with evaluation instruments and documents such as revising scores or answers on exams and scantrons. Fabrication – Falsifying experimental data or results, inventing research or laboratory data or results for work not done, or falsely claiming sources not used. Plagiarism – Representing someone else’s words, ideas, artistry, or data as one’s own, including copying another person’s work (including published and unpublished material, and material from the Internet) without appropriate referencing, presenting someone else’s opinions and theories as one’s own, or working jointly on a project, then submitting it as one’s own.

Assisting – Assisting another student in an act of academic dishonesty, such as taking a test or doing an assignment for someone else, changing someone’s grades or academic records, or inappropriately distributing exams or exam questions to other students.

I take all forms of academic dishonesty very seriously and will pursue the harshest penalties available from the college. You are all planning to be healthcare professionals and high standards of ethics are fundamental.

Withdraws You will not necessarily be dropped from this course if you quit attending class. It is your responsibility for withdrawing from the course once you have registered. To drop a class, you must go to the registrar and submit a drop card before the drop deadline or perform similar procedures by phone or online. Deadlines are set by the college and must be strictly followed. See Important Dates at the end of this syllabus

Constructive-Active Participation To get the most benefit from the course, you need to attend all the lectures. Your constructive-active participation during these times is essential to your ultimate success. You should arrive to class in a timely fashion and stay for the allotted time. Your grade can be negatively affected by poor constructive-active participation. It should be obvious that in order to participate fully in the class, you must be physically present in the classroom. Therefore, it is incumbent upon the student to be present and participate in all class meetings. Since this is expected of you, there will be no positive points assigned for this. However, if you do not meet expectations, points will be deducted in incremental fashion throughout the semester. If you are tardy at the beginning of class, after breaks, after lunch, or leave class early there will be a 1% deduction in grade for each occurrence. Missing a class with an unexcused absence will result in a 5% deduction. Except under extreme circumstances, the only viable excuses for missing class are religious holiday and participation in a college activity (such as an intercollegiate sport). You are responsible for all material presented and announcements made during class whether you are there or not. If you do not attend class, you will be at a severe disadvantage. Finally, if you miss the equivalent of more than two weeks of class or more, you may be dropped at the instructor’s discretion.

Tardiness I like to start on time without any interruptions so please prepare to start on time. I also give important information at the start of class and I will not repeat myself for a tardy student, as this is not fair to the other students. If you have problems or time conflicts which regularly prevent you from making it to class on time, you may need to seriously consider what your priorities are and make the appropriate change.

Electronic Devices 12

The use of cell phones, tablets and MP3 players is NOT permitted during class unless you are taping the lectures or using E-books. These cannot be used or displayed during exams for any reason. Please be respectful of your instructor and classmates by turning off all electronic devices before class begins. Using a cell phone for ANY reason during class (including texting) will result in a point deduction for each infraction. If you MUST use the cell phone, please notify me first.

Additional Considerations Excellent classroom demeanor is appreciated and expected from the students at all times. Talking during the lecture will not be tolerated as it is distracting to the instructor and other students. If you talk during lecture, you may be embarrassed by the instructor and an example made of you. If you are caught sleeping in class, you may be embarrassed by the instructor. If you are repeatedly caught sleeping in class, you will be asked to leave and a grade deduction will be made. In addition, no radios, compact disc players, cell phones or beepers should go off or be played in the room. Recorders are allowed for the purpose of recording the lectures.

Additional Information Inclement Weather/Emergency Situations There are a number of ways to find out if classes are canceled due to inclement weather. You will find complete information in the Inclement Weather and Emergency Procedure in the Student Handbook or on the New River website at the following link: http://www.newriver.edu/images/stories/hr/procedures/procedure_3- inclement_weather_1-2014.pdf.

New River Community and Technical College Social Justice Statement: “Consistent with its comprehensive mission and recognizing that the development of human potential is a fundamental goal in a democratic society, the Board of Governors for New River Community and Technical College promotes an educational system that values cultural and ethnic diversity and understanding; that provides for the preparation of students for full and meaningful participation in a changing world; and that promotes equitable and fair treatment in every aspect of campus life and employment for all persons regardless of race, national origin, gender, sexual preference, sexual orientation, age, religion, veteran status or disability.” Student Success Centers are located at each campus. Services provided are:  Tutoring - Individual, group and online options  Career Counseling  Academic Advising  Life Skills Advising  Disability Services  Placement Testing 13

 Make-up testing - appointment required, walk-ins will be accommodated when space is available  Study Groups  Workshops

ADA (Americans with Disabilities Act) Services Students with special needs are encouraged to contact the Educational Counselor in the Student Success Center on their campus before the start of the semester and request accommodations. If you have documented disabilities, you are eligible for ADA accommodations if they meet the conditions of Section 504 of the Rehabilitation Act of 1973 and the Americans with Disabilities Act of 1990. You must self-identify yourself and provide appropriate written documentation of your disability. If you qualify for services, you should work closely with the Educational Counselor to determine potential accommodations afforded to participate in the academic programs and services. Reasonable modifications in policies, practices, and procedures are implemented to assure equal access to individuals with disabilities. The Educational Counselor will prepare the “Notification of Need for Accommodation” letters for the faculty contact. Should you experience a temporary disability during the semester, you must provide reasonable and timely notice of a need for accommodations and services substantiated by appropriate documentation. Information provided is treated as confidential and is not released to anyone without your prior consent. Further information is available in the Student Handbook located on the College’s website at: www.newriver.edu/current-students/student-services/student-resources .

Veteran’s Priority Provisions: The Jobs for Veterans Act (Public Law 107-288( requires grantees to provide priority of service to veterans and spouses of certain veterans for the receipt of employment, training, and placement services in any job training program directly funded, in whole or in part, by DOL. The regulations implementing this priority of service can be found at 20 CFR part 1010. In circumstances where a grant recipient must choose between two qualified candidates for a service, one of whom is a veteran or eligible spouse, the veterans priority of service provisions require that the grant recipient give the veteran or eligible spouse priority of service by first providing him or her that service. To obtain priority of service, a veteran or spouse must meet the program’s eligibility requirements. Grantees must comply with DOL guidance on veterans’ priority. ETA’s Training and Employment Guidance Letter (TEGL) No. 10-09 (issued November 10, 2009) provides guidance on implementing priority of service for veterans and eligible spouses in qualified job training programs funded in whole or in part by DOL. TEGL No. 10-09 is available at http://wdr.doleta.gov/directives/corr_doc.cfm?DOCN=2816

Library Services 14

Library information services are available to all New River Community and Technical College students through the College’s Library located on the Greenbrier Valley Campus, Greenbrier County Public Library, the Summersville Public Library, the Raleigh County Public Library, the Concord University Library, the Princeton Public Library, the Craft Memorial Library in Bluefield, WV and the Bluefield State College Library. Each library is a member of the Mountain Library Network (MLN) and serves all MLN card holders. Library cards for these facilities are free and their collections are available through interlibrary loan. The Greenbrier Valley Campus library web page (http://www.newriver.edu/current-students/library- services) offers electronic library services to all users such as Academic OneFile (full text and summary peer-reviewed journal and popular magazine article databases and the Literature Resource Center) the Lexis-Nexis Academic (full text national and international newspaper articles, legal, business, and biographical databases), the Charleston Newspaper Index (full text), links to regional newspapers, CINAHL (nursing and allied health subjects), and Interlibrary Loan Request forms. MLN affiliated libraries offer journal databases through the West Virginia Library Commission. For further information and assistance, contact the College’s Librarian at (304) 647-6575.

Note: Changes in the course schedule may be necessary due to unforeseen circumstances. If changes become necessary, they will be announced in class and in Blackboard.

Advice To successfully tackle the area of science that we study, you need to sufficiently prepare yourself. Although there are many approaches to take, the most successful ones seem to have the following characteristics in common. The textbook should be read before coming to class. During class, be attentive, take good notes and participate in any discussions. Rewrite your notes before the next class period, making record of things you do not understand for future clarification. Each week, review all printed material so that you do not have to relearn it just before the exam. The general rule of thumb dictates that for every hour of class time, you should spend at least 2-3 hours outside of class studying. We meet for six hours per week – so outside of class you should spend a minimum of 12 hrs to 18 hrs. Many students find they do better if they regularly work with tutors or spend some of their study time working in groups. Use the four R’s: Read, Rewrite, Review, and Remember to help prepare for the exams. Finally, if you need help, ASK!

Four Rules to Remember 1. Neatness counts. 2. Organization and preparation is the key to life. 3. You will never get anywhere without hard work. 4. Treat others as you would like to be treated.

Important Dates to Remember

Jan 14 Classes begin Jan 18 Last day for schedule adjustment (Add/Drop) Mar 4 – 8 Mid-Semester Exams Mar 11 Mid-Semester grades due Mar 11-15 Spring Break – No classes Mar 18 – Apr 12 Registration for Summer 2019 & Fall 2019 for currently enrolled students Mar 25 Graduation applications for Summer 2019 due Apr 12 Last day to drop with a “W” May 3 Last day of classes May 6 – 10 Final exams May 11 Commencement May 14 Final Grades due

W Date Lecture - Chapter for Discussion Quiz / Test Lab k Reading Asmt 1 1-16 Course Orientation -Safety Review: Institute of Medicine Safety in Immunology & Serology Ch 6 (ihi.org) Learning from Med Errors, Part 1 Overview of Immunology Ch 1 -Pipetting Lab Basic Serological Techniques Ch 8 ImmuneQuest: www.immunequest.com Infectious Diseases (instructor notes) Ch 15 Use Code C3P2 for course Immunology & Infectious Mono (instructor notes) Ch 21 Immunohematology

MTS: Laboratory Methods Due 1-23 Overview of the Immune System (Youtube.com)

2 1-23 Antigens & Antibodies Ch 2 -Precipitation methods – Ouchterlony video Quality Assurance & Quality Control Ch 7 http://www.amrita.vlab.co.in Rapid Testing Ch 9 Precipitation & Agglutination Methods Ch 10 - ABO typing rgts (Agglutination test) MTS: Protein Electrophoresis Due 1-30 - Mono test (Agglutination test) - RPR (Flocculation test) - ASO (Agglutination test)

Introduction to how the immune system works (Youtube.com)

HHMI – video (Cells of the Immune System) http://www.hhmi.org/biointeractive

3 1-30 Cells of the Immune System- Ch 3 Electrophoresis: Aids Kit III-HIV detection Granulocytes & Mononuclear cells by Protein Electrophoresis Principles of Electrophoresis Ch 11 www.helena.com Strep Infections Ch 16 www.shsu.edu Cytomegalovirus Ch 20

ML: Mosquito-borne Viral Diseases -Serial Dilutions (use 1 mL anti-A rgt & 4% Du 2-6 RBCs for agglutination) or (blue or red food coloring to read on spec) www.wellesley.edu/Biology/Concepts/Html /serialdilutions.html

HHMI – video (Cloning an Army of T cells for Immune Defense) http://www.hhmi.org/biointeractive

4 2-6 Cells of the Immune System Ch 4 Lymphocytes & Plasma cells Syphilis Ch 17 Vector-borne Diseases Ch 18 Automated Procedures (videos) ML: Autoimmune Diseases & ANA Testing Due 2-13 ML: Tickborne Diseases Due 2-13

5 2-13 Soluable Mediators of the Immune Ch 5 Automated Procedures (demos) System Toxoplasmosis Ch 19 Immunoassay-Intro to ELISA (Edvotek) Hepatitis Ch 22 SLE Ch 28 HHMI – Virtual ELISA lab http://www.hhmi.org/biointeractive MTS: Intro to Molecular Diagnostics Print Virtual Lab Worksheet for students Due 2-20 6 2-20 Labeling Techniques in Immunoassay Ch 12 Molecular Techniques Molecular Techniques Ch 14 Alu Human DNA Typing using PCR Rheumatoid Arthritis Ch 29 (Edvotek)

ML: HIV-Structure, replication & detections Due 2-27 Immunology Final

7 2-27 QA, Regulation & Safety Ch 1 Making 2-5% cell suspensions Immunology Review for BB Ch 2 Grading Reactions BB Reagents & Applications Ch 3 MTS: Intro to Transfusion Svcs MTS: Transfusion Safety ML: Intro to the ABO Blood Group System ML & MTS due 3-20

8 3-6 Genetic Principles Ch 4 ABO/Rh Typing ABO & H Blood Group Systems & Ch 5 Secretors ML: ABO typing discrepancies Due 3-27 3-13 NO CLASS – SPRING BREAK 9 3-20 Rh Blood Group System Ch 6 Antibody Screen (IAT) Other RBC Groups Systems, HLA, Plt Ch 9 Compatibility Testing Antigens ML: Rh-Neg female with anti-D case stdy, Due 4-3 10 3-27 Antibody Detection and ID Ch 7 Antibody Screen (IAT) Compatibility Testing Ch 8 Antibody ID ML: Antibody Detection & ID Due 4-10

11 4-3 Adverse Complications of Transfusions Ch 11 Antibody ID Hemolytic Disease of the Fetus & Ch 12 Antigen Screening Newborn ML: Transfusion Rxns ML: Hemolytic Disease of the fetus & newbrn Due 4-17 12 4-10 Donor Selection & Phlebotomy Ch 13 HDFN screening Testing of Donor Blood Ch 14 DAT screening (Direct Coombs) ML: Disapperaring Ab-case stdy ML: TACO & allergic rxns Due 4-24 13 4-24 Blood Bank Automation Ch 10 Gel Procedure Review ML: Good Manufacturing Practices Due 5-10 18

14 5-1 Day for Catchup or Review Gel Procedure

15 5-8 FINAL FINAL  MTS = MedTraining  ML = MediaLab