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1 MLTN 230 Immunology/Immunohematology MLTN 230 Immunology/Immunohematology Lecture CRN 28365 (3-0-3) Semester: Spring 2019 Instructor: Diane Tyson, MS, MLS(ASCP), CLS(CA) Phone number: (304) 929-5038 E-mail address: [email protected] Class Hours and Location Lecture: Wed, 9 – 11:50 am, R135 Lab: Wed, 1 – 4:50 pm, Room RAH17, Allied Health Wing Office Location: R137, Raleigh County Campus Office Hours: Mon – by appt Tues – 11 am – 4 pm Thurs – 11 am to 4pm Fri – by appt Course Deliver Method: TRAD Help Desk: 304-929-6725 New River Vision Statement: New River Community and Technical College improves students’ lives and enriches communities. New River Mission Statement: New River Community and Technical College provides accessible, affordable, quality education and workforce programs to meet the needs of the region it serves. New River Social Justice Statement: Consistent with its comprehensive mission and recognizing that the development of human potential is a fundamental goal in a democratic society, the New River Community and Technical College Board of Governors promotes an educational system that values cultural and ethnic diversity and understanding; that provides for the preparation of students for full and meaningful participation in a changing world; and that promotes equitable and fair treatment in every aspect of campus life and employment for all persons regardless of race, national origin, gender, sexual preference, sexual orientation, age, religion, veteran status or disability. Course Description: This course is intended to prepare you with the knowledge, theory and skills to perform immunology, serology and immunohematology (transfusion and blood bank) analysis. You will also be trained in other important skills such as microscopy, ensuring patient safety, specimen collection and processing, quality control and assurance, critical thinking skills, communication skills and professional behavior. YOU MUST EARN A MINIMUM 75% IN BOTH 1 THE LECTURE AND LABORATORY TO PASS THIS COURSE AND PROCEED TO THE NEXT SEMESTER! You are allowed to retake the course (must take entire course) one time only if failing any portion of the course, lecture or lab. If you fail either portion of the course a second time, you will be dismissed from the program. Primary Textbook(s): Immunology & Serology in Laboratory Medicine, 6th ed. 2018, author Mary L. Turgeon. Publisher Mosby Elsevier. ISBN: 9780323431477 Basic & Applied Concepts of Blood Banking & Transfusion Practices, 4th ed, 2017, author, Paula Howard. Publisher Mosby Elsevier. ISBN: 9780323374781 Lab Manual: Provided by Instructor and/or posted on Blackboard. Publisher Online Student Resources: Evolve by Elsevier: www.evolve.elsevier.com Student resources our available on Blackboard, including power points, images and videos. University of Washington MedTraining: www.medtraining.org This is an online training and competency program. You will be required to complete these assignments continuously throughout the program. MediaLab: www.medialab.inc This is another powerful online training and competency program. You will be required to complete these assignments continuously throughout the program. MediaLab under Exam Review has exam reviews, practice exams and case studies that are accessible for all students throughout the MLT program. Outline IMMUNOLOGY AND IMMUNOHEMATOLOGY (3-0-3) Immunology Portion: (first 6 weeks) This is an introductory course in immunology designed for undergraduate medical laboratory technicians and medical laboratory science students. Lecture topics include: Antigens and antibodies Cells and cellular activities of the immune system Soluble mediators of immunity Quality Assurance & Quality Control Labeling Techniques in Immunoassay Basic Molecular Techniques Fundamentals The laboratory component of the course stresses a variety of methods, including: Basic Serological Laboratory Techniques Direct agglutination Precipitation Passive agglutination Immunodiffusion / gel diffusion Enzyme immunoassays Electrophoresis 2 Molecular techniques In addition to the basic principles of laboratory procedures, the course will address technical sources of error, procedural limitations, and clinical applications. Selected classic case studies will be presented to demonstrate the role of laboratory testing in the diagnosis and treatment of diseases. The overall goals of this course are: To introduce the student to the basic fundamentals of immunology and to apply this knowledge to selected clinical examples To familiarize the student with the underlying principles of serologic testing To introduce the student to the basic concepts of the immunologic response to infectious disease. COURSE OUTLINE: 1. Basic Immunologic Mechanisms a. Overview of Immunology b. Antigens and antibodies c. Cellular activity of the immune system - Granulocytes and Mononuclear cells - Lymphocytes and Plasma cells d. Soluble Mediators of the Immune System - Complement and the pathways - Cytokines, interleukins, interferons and other hematopoietic stimulators 2. Theory of Immunologic and Serologic Procedures a. Quality assurance and quality control b. Basic serologic techniques (dilutions) c. Point-of-Care Testing d. Agglutination methods e. Electrophoresis techniques, including Immunofixation electrophoresis (IFE) f. Labeling Techniques in Immunoassays g. Molecular techniques, including PCR, HIV-1 immunoblotting 3. Immunologic Manifestations of Infectious Diseases a. Viral disease - EBV / Infectious mononucleosis - Hepatitis - HIV / HTLV / CMV - Rubella / measles - Other viruses b. Microbial - Streptococcal infections - Cold agglutinins - Syphilis - Other microorganisms 3 c. Vector-borne diseases 4. Immunologically and Serologically Related Disorders a. Hypersensitivity Reactions b. Autoimmune disorders - ANA, anti-DNA, ENA - Systemic Lupus Erythematosus - Rheumatoid Arthritis c. Transplantation (Bone marrow and organ) Immunohematology Portion (last 8 weeks) This course in immunohematology (blood bank or transfusion services) is designed for undergraduate medical laboratory technicians and medical laboratory science students. Lecture topics include: Blood group antigen and antibody reactions Laboratory reagents for immunohematology Major blood group systems Essentials of pretransfusion testing Clinical considerations The laboratory portion of the course stresses Proper patient identification Principles of various testing in the blood bank Proper performance of testing in the blood bank Proper selection of blood units for patient transfusion In addition to the basic principles of laboratory procedures, the course will address technical sources of error, procedural limitations, and clinical applications. Selected classic case studies will be presented to demonstrate the role of laboratory testing in the clinical blood bank. The overall goals of this course are: To introduce the student to the basic fundamentals of immunohematology and to apply this knowledge to selected clinical examples To familiarize the student with the underlying principles of transfusion services testing COURSE OUTLINE 5. Basic Sciences and Reagents a. Basic principles and applications b. Blood banking reagents c. Genetic principles 6. Major Blood Groups a. ABO b. Rh 4 b. Lewis c. P d. Ii e. Duffy f. Kell g. Kidd h. MNS 7. Essentials of Pretransfusion Testing a. Antibody screening and identification b. Multiple antibodies c. Compatibility testing d. Special tests - DAT - Phenotyping and genotyping - Elution / absorption - Antibody titer - Pre-warm technique - Rosette 8. Blood Collection, Testing and Components a. Donor selection and requirements b. Blood Component Preparation c. Transfusion Therapy - RBC - Platelets - Fresh frozen plasma - Cryoprecipitated Anti-hemophilic factor - RhIg 9. Clinical considerations in Immunohematology a. Adverse complications of transfusions b. Hemolytic Disease of the Fetus and Newborn c. Transfusion Therapy in Selected Patients 10. Quality Assurance, Quality Control and Regulation of the Blood Industry Affective Outcomes: During the lecture, laboratory, clinical practicum, and upon completion of the program of study in Medical Laboratory Technology, the student will be able to demonstrate appropriate professional behavior, reliability and attitude skills. 1. Demonstrate professionalism by a. complying with the attendance policy, 5 b. complying with the dress code, c. submitting assignments by the stated deadline. 2. Demonstrate enthusiasm and interest in the profession by asking questions, participating in class discussions and meet with instructors during office hours as needed. 3. Demonstrate initiative by reviewing objectives and completing reading assignments prior to class. 4. Demonstrate progression in laboratory skills by effective organization, coordination of multiple tasks and insightful evaluation of results obtained. 5. Utilize constructive criticism to correct deficiencies and improve performance. 6. Work cooperatively with instructors and fellow students to achieve the goals of each activity. 7. Participate in activities designed to advance the profession of MLT and build professional pride. Lecture Cognitive /Psychomotor Outcomes: Upon completion of this course the student will be able to: 1. Describe the various quality assurance procedures and their uses that aid in the successful running of an Immunology and Immunohematology department. 2. Perform the basic tests that are performed in the Immunology and Immunohematology departments, such as syphilis and mono tests, blood typing, antibody
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