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Light and Electron Microscopy of the Exocrine Pancreas in the Chronically Reserpinized Rat1

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Light and Electron Microscopy of the Exocrine Pancreas in the Chronically Reserpinized Rat1 003 1 -3998/89/2505-0482$02.00/0 PEDIATRIC RESEARCH Vol. 25, No. 5, 1989 Copyright O 1989 International Pediatric Research Foundation, Inc. Printed rn U.S.A. Light and Electron Microscopy of the Exocrine Pancreas in the Chronically Reserpinized Rat1 GILLES GRONDIN, FRANCOIS A. LEBLOND, JEAN MORISSET, AND DENIS LEBEL Centre de Recherche sur Ies Micanismes de Sicrition, Faculty of Science, University of Sherbrooke, Sherbrooke, QC, Canada, JIK 2RI ABSTRACT. The effects of reserpine injections were stud- been the most studied (1-8). The main effects induced by the ied on the morphology of the pancreas in an experimental reserpine treatment on the ultrastructure of the pancreas are the model for cystic fibrosis, the chronically reserpinized rat. following: an accumulation of granules in the acinar cell, an A detailed examination of the tissue was carried out at the alteration of the granule ultrastructure, a slight diminution of light and electron microscopic levels. The nonspecific ef- the RER and Golgi apparatus, the presence of autophagic bodies, fects of secondary malnutrition induced by the drug were and an augmentation of lysosomal bodies (3,5,9, 10). In another assessed with a group of animals pair fed with the treated paper (1 I), we report the effects of such a treatment on pancreatic animals. In a companion paper, we show that pancreatic growth and on the amount of a glycoprotein characteristic of the wt, lipase, and GP-2 contents also are affected by reserpine zymogen granule membrane, the GP-2. In this study, we specif- treatment. In this study, we report that no morphologic ically report the effects of chronic reserpine treatment at two differences were observed between the exocrine pancreatic doses, on the structure and ultrastructure of the rat pancreas. tissue of control and pair-fed animals. By contrast, reser- The effect of malnutrition, which is also induced by this treat- pine induced an accumulation of zymogen granules in 60% ment, was evaluated by including a group of pair-fed animals. of the treated animals and a concomitant decrease of the The study is a thorough illustration, at the light and electron area occupied by the rough endoplasmic reticulum in the microscopic levels, of the transformations induced in the rat same cells. Finally, in all treated animals, at the light and exocrine pancreas by chronic reserpine treatment. electron microscopic levels, it was observed that some particular regions of the pancreatic tissue were strongly affected. In these regions, numerous autophagic bodies and MATERIALS AND METHODS lysosomes were observed. Cisternae of the Golgi complex Four groups of five male Sprague-Dawley rats (200-275 g) were also more distended. Some acinar cells were in the were used in this study. The first two groups were injected daily process of lysis. Several vacuolar inclusions were present intrapentoneally with reserpine at 0.5 or 1.0 mg.kg-' in a in some intralobular duct cells. Cellular material was seen solution of benzyl alcohol, polyethylene glycol 300, and acetic in acinar and intralobular duct lumina. In these same acid (Martinez JR, personal communication). As discussed in regions, distended intralobular ducts and acinar lumina another article (1 I), rats in the control group were injected with were observed. These last two features have never been the vehicle used to dissolve the drug. The fourth group of rats reported in the reserpinized rat but are important manifes- was injected as the control group but received an amount of food tations of the pathology in cystic fibrosis patients where equal to that ingested the day before by the group treated with obstructions of ducts are believed to trigger focal destruc- reserpine at 0.5 mgkg-'. This constituted the group of pair-fed tion of the pancreatic tissue. These observations, along animals used to evaluate the effect of malnutrition induced by with the three parameters previously reported to be spe- the treatment. All other animals received water and food (pow- cifically altered by reserpine injections, clearly support the dered rat food) ad libitum. use of the chronically reserpinized rat as a reliable model After 7 d of treatment, animals were fasted overnight before for the study of cystic fibrosis. (Pediatr Res 25:482-489, being killed by decapitation. Immediately after they were killed, 1989) three pieces of tissue were taken from different regions of the pancreas on each animal and fixed for 60 min in 2% glutaral- Abbreviations dehyde buffered with Na cacodylate, pH 7.2. Tissue segments CF, cystic fibrosis were postfixed for 60 min in 2% osmium tetroxide in the same RER, rough endoplasmic reticulum buffer. Specimens were finally dehydrated and embedded in GP-2, the major protein of the pancreatic zymogen granule Epon 812 according to standard procedures. The remaining membrane tissue was used for biochemical determinations as already re- ported (I 1). For each piece of tissue, five l-pm thick sections were stained with toluidine blue for light microscopic examination. Micro- Because of histologic, physiologic, and biochemical similarities graphs were taken with a standard Universal Zeiss microscope equipped with an orange filter on Kodak 32 ASA Panatomic existing between reserpine-treated animals and CF patients, the film. Electron microscopy was performed on thin, 70-nm thick chronically reserpinized rat is the animal model of CF that has sections stained with uranyl acetate and lead citrate. Observations Received July 11, 1988; accepted December 5, 1988. were done on a Philips 20 1 electron microscope. Correspondence and reprint requests Dr. Denis LeBel, Department of Biology, Although our objective in this study was not to conduct a University of Sherbrooke, Sherbrooke, QC, Canada, J I K 2R I. morphometric evaluation, we were very concerned about the Supported by a grant from the Canadian Cystic Fibrosis Foundation and NSERC to D.L. D.L. is a NSERC of Canada Research Associate. choice of plane of section to assess differences in size and ' This is the 10th paper in a series "Elucidation of the Mechanisms of Cellular quantity. We photographed what we considered representative Secretion." areas that would allow comparisons of cells in similar orientation. 7.q ".,:- -7% 1.. b c -yG--%<.:- * .. ; 9,- >#* - +*,*: 2 4, L s...: .. *-:,-" . = >+-, cl .,, p;y5. **, &;.I .; i..,... ,<%; -* 'd'. ' .'.?. 4. $4 *- ..--. ., ,:*-.*.+J+ ?I2^. Fig. 1 and 2. Light microscopy of exocrine pancreatic tissue of control, reserpine-treated, and pair-fed rats (1 325x). Pancreatic tissues of pair fed (16) rats show no histologic differences with control (la) rats. Granules occupy about one-third of the cell surface. In pancreas of rats treated durinj: 7 d by daily intraperitoneal injection with 0.5 (2a) or 1.0 (2b) mg. kg-' of reserpine, granules are more numerous and occupy about two-thirds of the cell. Of treated animals, 40% show a normal number of granules (Zc, at 1.0 mgkg-'). In all treated animals, some acinar lumina (arrowheads) and intralobular ducts (arrow) are distended (Zd, at 0.5 mg.kg-I). 484 GRONDIN ET AL. RESULTS Electron microscopy. In general, observations done at the electron microscopic level confirmed in more details the obser- vations made with the light microscope. The ultrastructure of Light microscopy. The pancreatic tissue of pair-fed animals the pancreatic exocrine cell of control and pair-fed animals were did not show any histological differences from sham-injected similar. An example of the ultrastructure of the pancreatic acinar control animals. As shown in Figure 1, the structure of the cell of pair-fed animals is shown in Figure 4a. One can observe exocrine pancreas was normal. The nucleus is located in the that the RER and the Golgi complex are well developed, that basal region, the RER is well developed, and zymogen granules the granulation is uniform, and that acinar lumen contain many occupy approximately one-third of the cell surface. The pancreas microvilli. By contrast, alterations in the acinar cell ultrastructure of animals treated with any of the two doses of reserpine had a of all reserpinized animals were similar. No dose-dependent very similar histology when compared to each other (Fig. 2a and effects of the drug could be evaluated by such morphologic b). When compared to the control however, 60% of the treated examination. As put in evidence with light microscopy, some rats showed more secretion granules occupying approximately regions of the gland of treated animals were normal and identical two-thirds of the cell. The remaining 40% of the animals had an to controls except, as previously mentioned, for the amount of amount of granules in the pancreas similar to the sham-injected zymogen granules in some animals. Other regions of the tissue, control (Fig. 2c). Dilated acinar and intralobular lumina were however, displayed major alterations. The following descriptions observed in certain regions of the pancreatic tissue in all treated of abnormalities are therefore restricted to regions of the pan- animals (Fig. 2d). Detailed inspection of these areas revealed creatic tissue affected by the treatment. As shown in Figure 4b, more particularly the presence of autophagic bodies in basolateral Golgi cisternae and acinar lumen are clearly dilated when com- regions of acinar cells (Fig. 3a). These structures were also pared to pair-fed tissue. The lumen appears expanded and con- observed at the exterior of the cell (Fig. 3b). Cellular material tains few microvilli. Material present in these lumen are of a was also found in intralobular ducts (Fig. 3c). Consistently ob- rather faint density to electrons. This swelling can be more served in these particular regions bordering intralobular ducts accurately appreciated at higher magnification when compared were acinar cells in the process of lysis (Fig. 3d and e). In to a normal acinar lumen (Fig. 4c and d).The same phenomenon intralobular ducts, some cells contained numerous vacuoles (Fig.
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