DOCSLIB.ORG

Systematics of Coccal Green Algae of the Classes Chlorophyceae and Trebouxiophyceae

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Systematics of Coccal Green Algae of the Classes Chlorophyceae and Trebouxiophyceae School of Doctoral Studies in Biological Sciences University of South Bohemia in České Budějovice Faculty of Science SYSTEMATICS OF COCCAL GREEN ALGAE OF THE CLASSES CHLOROPHYCEAE AND TREBOUXIOPHYCEAE Ph.D. Thesis Mgr. Lenka Štenclová Supervisor: Doc. RNDr. Jan Kaštovský, Ph.D. University of South Bohemia in České Budějovice České Budějovice 2020 This thesis should be cited as: Štenclová L., 2020: Systematics of coccal green algae of the classes Chlorophyceae and Trebouxiophyceae. Ph.D. Thesis Series, No. 20. University of South Bohemia, Faculty of Science, School of Doctoral Studies in Biological Sciences, České Budějovice, Czech Republic, 239 pp. Annotation Aim of the review part is to summarize a current situation in the systematics of the green coccal algae, which were traditionally assembled in only one order: Chlorococcales. Their distribution into the lower taxonomical unites (suborders, families, subfamilies, genera) was based on the classic morphological criteria as shape of the cell and characteristics of the colony. Introduction of molecular methods caused radical changes in our insight to the system of green (not only coccal) algae and green coccal algae were redistributed in two of newly described classes: Chlorophyceae a Trebouxiophyceae. Representatives of individual morphologically delimited families, subfamilies and even genera and species were commonly split in several lineages, often in both of mentioned classes. For the practical part, was chosen two problematical groups of green coccal algae: family Oocystaceae and family Scenedesmaceae - specifically its subfamily Crucigenioideae, which were revised using polyphasic approach. Based on the molecular phylogeny, relevance of some old traditional morphological traits was reevaluated and replaced by newly defined significant characteristics. Declaration [in Czech] Prohlašuji, že svoji disertační práci jsem vypracovala samostatně pouze s použitím pramenů a literatury uvedených v seznamu citované literatury. Prohlašuji, že v souladu s § 47b zákona č. 111/1998 Sb. v platném zněni souhlasím se zveřejněním své disertační práce, a to v nezkrácené podobě elektronickou cestou ve veřejně přístupné časti databáze STAG provozované Jihočeskou univerzitou v Českých Budějovicích na jejich internetových stránkách, a to se zachováním mého autorského práva k odevzdanému textu teto kvalifikační práce. Souhlasím dále s tím, aby toutéž elektronickou cestou byly v souladu s uvedeným ustanovením zákona č. 111/1998 Sb. zveřejněny posudky školitele a oponentů práce i záznam o průběhu a výsledku obhajoby kvalifikační práce. Rovněž souhlasím s porovnáním textu mé kvalifikační práce s databází kvalifikačních prací Theses.cz provozovanou Národním registrem vysokoškolských kvalifikačních prací a systémem na odhalovaní plagiátů. České Budějovice 3. 11. 2020 Lenka Štenclová This thesis originated from Faculty of Science, University of South Bohemia, supporting doctoral studies in the Botany study program. Financial support: Financial support came from the budged of the Department of Botany (RVO) and partly from Assumption College Faculty Development Grant. Acknowledgements: On the very first place I thank to my supervisor Hanys for not stopping believing at this project, for his support and great ideas. I owe my thanks to Karolina Fučíková as well, for her friendly cooperation on Assumption college. I thank my colleagues and friends at the same time from České Budějovice for valuable advices and all their funny talks that this is thesis full of small green balls looking all alike. My special thanks belong to my parents for their kind support. List of papers and author’s contribution The thesis is based on the following papers (listed according to the thematic chapters): Štenclová, L., Fučíková, K., Kaštovský, J. and Pažoutová, M. (2017) Molecular and morphological delimitation and generic classification of the family Oocystaceae (Trebouxiophyceae, Chlorophyta). Journal of Phycology. 53(6):1263-1282. (IF 2017 = 3.000) Lenka Štenclová cultivated strains, performed molecular methods and molecular phylogeny, morphological and ultrastructural (TEM) observations, and was main author of the manuscript. Silva, T. G., Štenclová, L., Archanjo, N.C.P. and Bagatini, C. L. Revised phylogenetic position of genus Nephrocytium Nägeli (Sphaeropleales, Chlorophyceae), with description of Nephrocytiaceae fam. nov. and Nephrocytium vieirae sp. nov. (Manuscript) Lenka Štenclová cultivated one strain, performed molecular methods, morphological and ultrastructural (TEM) observations, and was second author of the manuscript. Štenclová, L. Distribution of the Crucigenioid algae inside the classes Chlorophyceae and Trebouxiophyceae. (Manuscript) Lenka Štenclová cultivated strains, performed molecular methods and molecular phylogeny, morphological observations, and was only author of the manuscript. Štenclová, L. and Fučíková, K. (2019) Dispora speciosa, a new addition to the genus Parallela and the first coccoid member of the family Microsporaceae. Phytotaxa. 419(1):63-76. DOI:10.11646/phytotaxa.419.1.4 (IF 2018/19 = 1.168) Lenka Štenclová cultivated strain, performed molecular methods, morphological and ultrastructural observations, and was main author of the manuscript. Contents Chapter 1: Introduction: Situation in current systematics of green coccal algae............... 1 1.1 Traditional morphological system .......................................................................... 2 1.2. Ultrastructural research challenging the morphological system ............................ 5 1.3. Modern system of green algae based on the polyphasic approach ........................ 7 1.4. Coccal algae inside classes Trebouxiophyceae a Chlorophyceae........................ 10 1.5. Ongoing problems in current systematics of green coccal algae ......................... 15 1.6. Phylogenetic methods as the backbone for modern systematics ......................... 18 1.7. Aims of the thesis ................................................................................................ 20 1.8. Results ................................................................................................................. 21 1.8.1 Molecular and morphological delimitation and generic classification of the family Oocystaceae ........................................................................................ 22 1.8.2. Revised phylogenetic position of genus Nephrocytium Nägeli (Sphaeropleales, Chlorophyceae), with description of Nephrocytiaceae fam. nov. and Nephrocytium vieirae sp. nov. ............................................................... 23 1.8.3. Distribution of Crucigenioid algae in classes Chlorophyceae & Trebouxiophyceae ................................................................................................ 24 1.8.4 Dispora speciosa, a new addition to the genus Parallela and the first coccoid member of the family Microsporaceae ................................................. 26 1.9. Conclusion ........................................................................................................... 26 1.10. Following problems ........................................................................................... 27 1.10.1. Delimitation and species concept of the genus Oocystis ........................ 27 1.10.2. Phylogenetic position of Juraniella javorkae and new insides in the phylogeny of the genus Kirchneriella ................................................................. 28 1.10.3. Characterizing the chloroplast and mitochondrial genomes of a microscopic alga Oonephris obesa (Chlorophyceae) ......................................... 28 1.11. References ......................................................................................................... 30 Chapter 2 Molecular and morphological delimitation and generic classification of the family Oocystaceae (Trebouxiophyceae, Chlorophyta) ............................................ 45 Chapter 3 Revised phylogenetic position of genus Nephrocytium Nägeli (Sphaeropleales, Chlorophyceae), with description of Nephrocytiaceae fam. nov. and Nephrocytium vieirae sp. nov. ....................................................................................... 103 Chapter 4 Distribution of Crucigenioid algae in classes Chlorophyceae & Trebouxiophyceae ......................................................................................................... 107 Chapter 5 Dispora speciosa, a new addition to the genus Parallela and the first coccoid member of the family Microsporaceae ............................................................ 111 Authors CV ……………………………………………………………………….145 Chapter 1: Introduction: Situation in current systematics of green coccal algae Pröschold and Leliaert 2007 1 Chapter 1: Situation in current systematics of green coccal algae 1.1 Traditional morphological system Coccal thallus is the simplest organization of the algal body because the entire organism consists of simply one cell. Coccal thallus covered by visible mucilage wrap is called palmeloid or also capsal. Both types can agglomerate into regular or irregular colonies and coenobia. All the coccal and palmeloid green algae were, according to the traditional morphological system summarized in the last comprehensive morphological monograph by Komárek and Fott (1983), assembled in a single order Chlorococcales, just on the basis of the common body organization Komárek and Fott (1983), expecting its one time evolution from a green flagellate ancestor common for
Load more

Recommended publications
  • Phylogenetic Placement of Botryococcus Braunii (Trebouxiophyceae) and Botryococcus Sudeticus Isolate Utex 2629 (Chlorophyceae)1
    J. Phycol. 40, 412–423 (2004) r 2004 Phycological Society of America DOI: 10.1046/j.1529-8817.2004.03173.x PHYLOGENETIC PLACEMENT OF BOTRYOCOCCUS BRAUNII (TREBOUXIOPHYCEAE) AND BOTRYOCOCCUS SUDETICUS ISOLATE UTEX 2629 (CHLOROPHYCEAE)1 Hoda H. Senousy, Gordon W. Beakes, and Ethan Hack2 School of Biology, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK The phylogenetic placement of four isolates of a potential source of renewable energy in the form of Botryococcus braunii Ku¨tzing and of Botryococcus hydrocarbon fuels (Metzger et al. 1991, Metzger and sudeticus Lemmermann isolate UTEX 2629 was Largeau 1999, Banerjee et al. 2002). The best known investigated using sequences of the nuclear small species is Botryococcus braunii Ku¨tzing. This organism subunit (18S) rRNA gene. The B. braunii isolates has a worldwide distribution in fresh and brackish represent the A (two isolates), B, and L chemical water and is occasionally found in salt water. Although races. One isolate of B. braunii (CCAP 807/1; A race) it grows relatively slowly, it sometimes forms massive has a group I intron at Escherichia coli position 1046 blooms (Metzger et al. 1991, Tyson 1995). Botryococcus and isolate UTEX 2629 has group I introns at E. coli braunii strains differ in the hydrocarbons that they positions 516 and 1512. The rRNA sequences were accumulate, and they have been classified into three aligned with 53 previously reported rRNA se- chemical races, called A, B, and L. Strains in the A race quences from members of the Chlorophyta, includ- accumulate alkadienes; strains in the B race accumulate ing one reported for B.
    [Show full text]
  • Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016
    Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016 April 1981 Revised, May 1982 2nd revision, April 1983 3rd revision, December 1999 4th revision, May 2011 Prepared for U.S. Department of Commerce Ohio Department of Natural Resources National Oceanic and Atmospheric Administration Division of Wildlife Office of Ocean and Coastal Resource Management 2045 Morse Road, Bldg. G Estuarine Reserves Division Columbus, Ohio 1305 East West Highway 43229-6693 Silver Spring, MD 20910 This management plan has been developed in accordance with NOAA regulations, including all provisions for public involvement. It is consistent with the congressional intent of Section 315 of the Coastal Zone Management Act of 1972, as amended, and the provisions of the Ohio Coastal Management Program. OWC NERR Management Plan, 2011 - 2016 Acknowledgements This management plan was prepared by the staff and Advisory Council of the Old Woman Creek National Estuarine Research Reserve (OWC NERR), in collaboration with the Ohio Department of Natural Resources-Division of Wildlife. Participants in the planning process included: Manager, Frank Lopez; Research Coordinator, Dr. David Klarer; Coastal Training Program Coordinator, Heather Elmer; Education Coordinator, Ann Keefe; Education Specialist Phoebe Van Zoest; and Office Assistant, Gloria Pasterak. Other Reserve staff including Dick Boyer and Marje Bernhardt contributed their expertise to numerous planning meetings. The Reserve is grateful for the input and recommendations provided by members of the Old Woman Creek NERR Advisory Council. The Reserve is appreciative of the review, guidance, and council of Division of Wildlife Executive Administrator Dave Scott and the mapping expertise of Keith Lott and the late Steve Barry.
    [Show full text]
  • Neoproterozoic Origin and Multiple Transitions to Macroscopic Growth in Green Seaweeds
    Neoproterozoic origin and multiple transitions to macroscopic growth in green seaweeds Andrea Del Cortonaa,b,c,d,1, Christopher J. Jacksone, François Bucchinib,c, Michiel Van Belb,c, Sofie D’hondta, f g h i,j,k e Pavel Skaloud , Charles F. Delwiche , Andrew H. Knoll , John A. Raven , Heroen Verbruggen , Klaas Vandepoeleb,c,d,1,2, Olivier De Clercka,1,2, and Frederik Leliaerta,l,1,2 aDepartment of Biology, Phycology Research Group, Ghent University, 9000 Ghent, Belgium; bDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Zwijnaarde, Belgium; cVlaams Instituut voor Biotechnologie Center for Plant Systems Biology, 9052 Zwijnaarde, Belgium; dBioinformatics Institute Ghent, Ghent University, 9052 Zwijnaarde, Belgium; eSchool of Biosciences, University of Melbourne, Melbourne, VIC 3010, Australia; fDepartment of Botany, Faculty of Science, Charles University, CZ-12800 Prague 2, Czech Republic; gDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742; hDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138; iDivision of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, United Kingdom; jSchool of Biological Sciences, University of Western Australia, WA 6009, Australia; kClimate Change Cluster, University of Technology, Ultimo, NSW 2006, Australia; and lMeise Botanic Garden, 1860 Meise, Belgium Edited by Pamela S. Soltis, University of Florida, Gainesville, FL, and approved December 13, 2019 (received for review June 11, 2019) The Neoproterozoic Era records the transition from a largely clear interpretation of how many times and when green seaweeds bacterial to a predominantly eukaryotic phototrophic world, creat- emerged from unicellular ancestors (8). ing the foundation for the complex benthic ecosystems that have There is general consensus that an early split in the evolution sustained Metazoa from the Ediacaran Period onward.
    [Show full text]
  • Diversity of the Genera of Chlorophyta in Fresh Waters of District Swat Nwfp
    Pak. J. Bot., 43(3): 1759-1764, 2011. DIVERSITY OF THE GENERA OF CHLOROPHYTA IN FRESH WATERS OF DISTRICT SWAT N.W.F.P PAKISTAN ASGHAR ALI1, ZABTA KHAN SHINWARI2 AND MUHAMMAD KHAN LEGHARI3 1Department of Botany, G.P.G. Jahanzeb College Swat, Pakistan 2Department of Biotechnology, Quaid-e-Azam University Islamabad, Pakistan 3Pakistan Museum of Natural History, Islamabad, Pakistan Abstract Fifty six genera of green algae were collected from ten different localities of District Swat, belonging to 25 families and 9 genera of Chlorophyta from December 2006 August 2008. Family Oocystaceae with 39 species was most commonly found, next to it were families Scenedesmaceae with18 species and Desmidiaceae with 14 species. The genera Oocystis and Tetraedron were represented by 10 species and Cosmarium with 7 species occurred most commonly. Among the recorded genera 13 (23.2%) were Unicellular, 25 (44.6%) were Colonial, 9 (16.7%) were Unbranched filamentous, 4 (7.1%) were branched filamentous, 1 (1.7%) was Pseudofilamentous, 1 (1.7%) was Mesh-like, 2 (3.5%) were Heterotrichous and 1 (1.7%) was with Irregular amorphous thallus. Highest proportion of Chlorophycean members was recorded from Kanju area 89 and lowest was recorded from Kalam 69. Introduction Results and Discussion The Valley of Swat a part of Malakand Division covers Fifty six genera containing 138 species belonging to 25 5737 square kilometers (estimated). The elevation of the families and 9 orders have been collected from various fresh valley is 630 to 3000m above sea level. Swat is located at a water habitats. Collected algal members were identified up to distance of 170 km from Peshawar and 270 km from Federal species level.
    [Show full text]
  • Multiple Origins of Endosymbionts in Chlorellaceae with No Reductive
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln James Van Etten Publications Plant Pathology Department 8-30-2017 Multiple origins of endosymbionts in Chlorellaceae with no reductive effects on the plastid or mitochondrial genomes Weishu Fan University of Nebraska-Lincoln, [email protected] Wenhu Guo University of NebrWuhan Frasergen Bioinformatics Co. Ltd., Wuhanaska - Lincoln James L. Van Etten University of Nebraska-Lincoln, [email protected] Jeffrey P. Mower University of Nebraska-Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/vanetten Part of the Genetics and Genomics Commons, Plant Pathology Commons, and the Viruses Commons Fan, Weishu; Guo, Wenhu; Van Etten, James L.; and Mower, Jeffrey P., "Multiple origins of endosymbionts in Chlorellaceae with no reductive effects on the plastid or mitochondrial genomes" (2017). James Van Etten Publications. 24. https://digitalcommons.unl.edu/vanetten/24 This Article is brought to you for free and open access by the Plant Pathology Department at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in James Van Etten Publications by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. www.nature.com/scientificreports OPEN Multiple origins of endosymbionts in Chlorellaceae with no reductive efects on the plastid or Received: 14 March 2017 Accepted: 8 August 2017 mitochondrial genomes Published: xx xx xxxx Weishu Fan1,2, Wenhu Guo3, James L. Van Etten4 & Jefrey P. Mower1,2 Ancient endosymbiotic relationships have led to extreme genomic reduction in many bacterial and eukaryotic algal endosymbionts. Endosymbionts in more recent and/or facultative relationships can also experience genomic reduction to a lesser extent, but little is known about the efects of the endosymbiotic transition on the organellar genomes of eukaryotes.
    [Show full text]
  • Altitudinal Zonation of Green Algae Biodiversity in the French Alps
    Altitudinal Zonation of Green Algae Biodiversity in the French Alps Adeline Stewart, Delphine Rioux, Fréderic Boyer, Ludovic Gielly, François Pompanon, Amélie Saillard, Wilfried Thuiller, Jean-Gabriel Valay, Eric Marechal, Eric Coissac To cite this version: Adeline Stewart, Delphine Rioux, Fréderic Boyer, Ludovic Gielly, François Pompanon, et al.. Altitu- dinal Zonation of Green Algae Biodiversity in the French Alps. Frontiers in Plant Science, Frontiers, 2021, 12, pp.679428. 10.3389/fpls.2021.679428. hal-03258608 HAL Id: hal-03258608 https://hal.archives-ouvertes.fr/hal-03258608 Submitted on 11 Jun 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. fpls-12-679428 June 4, 2021 Time: 14:28 # 1 ORIGINAL RESEARCH published: 07 June 2021 doi: 10.3389/fpls.2021.679428 Altitudinal Zonation of Green Algae Biodiversity in the French Alps Adeline Stewart1,2,3, Delphine Rioux3, Fréderic Boyer3, Ludovic Gielly3, François Pompanon3, Amélie Saillard3, Wilfried Thuiller3, Jean-Gabriel Valay2, Eric Maréchal1* and Eric Coissac3* on behalf of The ORCHAMP Consortium 1 Laboratoire de Physiologie Cellulaire et Végétale, CEA, CNRS, INRAE, IRIG, Université Grenoble Alpes, Grenoble, France, 2 Jardin du Lautaret, CNRS, Université Grenoble Alpes, Grenoble, France, 3 Université Grenoble Alpes, Université Savoie Mont Blanc, CNRS, LECA, Grenoble, France Mountain environments are marked by an altitudinal zonation of habitat types.
    [Show full text]
  • Lateral Gene Transfer of Anion-Conducting Channelrhodopsins Between Green Algae and Giant Viruses
    bioRxiv preprint doi: https://doi.org/10.1101/2020.04.15.042127; this version posted April 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 5 Lateral gene transfer of anion-conducting channelrhodopsins between green algae and giant viruses Andrey Rozenberg 1,5, Johannes Oppermann 2,5, Jonas Wietek 2,3, Rodrigo Gaston Fernandez Lahore 2, Ruth-Anne Sandaa 4, Gunnar Bratbak 4, Peter Hegemann 2,6, and Oded 10 Béjà 1,6 1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel. 2Institute for Biology, Experimental Biophysics, Humboldt-Universität zu Berlin, Invalidenstraße 42, Berlin 10115, Germany. 3Present address: Department of Neurobiology, Weizmann 15 Institute of Science, Rehovot 7610001, Israel. 4Department of Biological Sciences, University of Bergen, N-5020 Bergen, Norway. 5These authors contributed equally: Andrey Rozenberg, Johannes Oppermann. 6These authors jointly supervised this work: Peter Hegemann, Oded Béjà. e-mail: [email protected] ; [email protected] 20 ABSTRACT Channelrhodopsins (ChRs) are algal light-gated ion channels widely used as optogenetic tools for manipulating neuronal activity 1,2. Four ChR families are currently known. Green algal 3–5 and cryptophyte 6 cation-conducting ChRs (CCRs), cryptophyte anion-conducting ChRs (ACRs) 7, and the MerMAID ChRs 8. Here we 25 report the discovery of a new family of phylogenetically distinct ChRs encoded by marine giant viruses and acquired from their unicellular green algal prasinophyte hosts.
    [Show full text]
  • The Draft Genome of Hariotina Reticulata (Sphaeropleales
    Protist, Vol. 170, 125684, December 2019 http://www.elsevier.de/protis Published online date 19 October 2019 ORIGINAL PAPER Protist Genome Reports The Draft Genome of Hariotina reticulata (Sphaeropleales, Chlorophyta) Provides Insight into the Evolution of Scenedesmaceae a,b,2 c,d,2 b e f Yan Xu , Linzhou Li , Hongping Liang , Barbara Melkonian , Maike Lorenz , f g a,g e,1 a,g,1 Thomas Friedl , Morten Petersen , Huan Liu , Michael Melkonian , and Sibo Wang a BGI-Shenzhen, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China b BGI Education Center, University of Chinese Academy of Sciences, Beijing, China c China National GeneBank, BGI-Shenzhen, Jinsha Road, Shenzhen 518120, China d Department of Biotechnology and Biomedicine, Technical University of Denmark, Copenhagen, Denmark e University of Duisburg-Essen, Campus Essen, Faculty of Biology, Universitätsstr. 5, 45141 Essen, Germany f Department ‘Experimentelle Phykologie und Sammlung von Algenkulturen’ (EPSAG), University of Göttingen, Nikolausberger Weg 18, 37073 Göttingen, Germany g Department of Biology, University of Copenhagen, Copenhagen, Denmark Submitted October 9, 2019; Accepted October 13, 2019 Hariotina reticulata P. A. Dangeard 1889 (Sphaeropleales, Chlorophyta) is a common member of the summer phytoplankton of meso- to highly eutrophic water bodies with a worldwide distribution. Here, we report the draft whole-genome shotgun sequencing of H. reticulata strain SAG 8.81. The final assembly comprises 107,596,510 bp with over 15,219 scaffolds (>100 bp). This whole-genome project is publicly available in the CNSA (https://db.cngb.org/cnsa/) of CNGBdb under the accession number CNP0000705. © 2019 Elsevier GmbH. All rights reserved. Key words: Scenedesmaceae; genome; algae; comparative genomics.
    [Show full text]
  • Abstract Resumen
    KATIA ANCONA-CANCHÉ1, SILVIA LÓPEZ-ADRIÁN2, MARGARITA ESPINOSA-AGUILAR3, GLORIA GARDUÑO-SOLÓRZANO4, TANIT TOLEDANO-THOMPSON1, JOSÉ NARVÁEZ- ZAPATA5 AND RUBY VALDEZ-OJEDA1 Botanical Sciences 95 (3): 527-537, 2017 Abstract Background: Scenedesmaceae family exhibits great morphological variability. High phenotypic plasticity and the pres- DOI: 10.17129/botsci.1201 ence of cryptic species have resulted in taxonomic re-assignments of Scenedesmaceae members. Study strains: Strains CORE-1, CORE-2 and CORE-3 were characterized. Copyright: © 2017 Ancona-Canché Study site: Yucatan Peninsula et al. This is an open access article Methods: Morphological analyses were executed by optical and scanning electron microscopy. Phylogenetic relation- distributed under the terms of the ships were examined by ITS-2 and ITS1-5.8S-ITS2 rDNA regions. Creative Commons Attribution Li- cense, which permits unrestricted Results: Optical and scanning electron microscopy analyses indicated spherical to ellipsoidal cells and autospore for- use, distribution, and reproduction mation correspond to members of the family Scenedesmaceae, as well as observable pyrenoid starch plates. Detailed in any medium, provided the original morphology analysis indicated that CORE-1 had visible granulations dispersed on the cell wall, suggesting identity with author and source are credited. Verrucodesmus verrucosus. However CORE-1 did not show genetic relations with this species, and was instead clus- tered close to the genus Coelastrella. CORE-2 did not show any particular structure or ornamentation, but it did show genetic relations with Coelastrella with good support. CORE-3 showed meridional ribs from end to end, one of them forked and well pronounced, and orange cells in older cultures characteristic of Coelastrella specimens.
    [Show full text]
  • Diapositive 1
    1 Diversity and ecology of green microalgae in marine systems: 2 an overview based on 18S rRNA sequences 3 4 Margot Tragin1, Adriana Lopes dos Santos1, Richard Christen2, 3, Daniel Vaulot1* 5 1 Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 7144, Station Biologique, Place 6 Georges Teissier, 29680 Roscoff, France 7 2 CNRS, UMR 7138, Systématique Adaptation Evolution, Parc Valrose, BP71. F06108 Nice 8 cedex 02, France 9 3 Université de Nice-Sophia Antipolis, UMR 7138, Systématique Adaptation Evolution, Parc 10 Valrose, BP71. F06108 Nice cedex 02, France 11 12 13 * corresponding author : [email protected] 14 Revised version : 28 March 2016 15 For Perspectives in Phycology 16 17 Tragin et al. - Marine Chlorophyta diversity and distribution - p. 2 18 Abstract 19 Green algae (Chlorophyta) are an important group of microalgae whose diversity and ecological 20 importance in marine systems has been little studied. In this review, we first present an overview of 21 Chlorophyta taxonomy and detail the most important groups from the marine environment. Then, using 22 public 18S rRNA Chlorophyta sequences from culture and natural samples retrieved from the annotated 23 Protist Ribosomal Reference (PR²) database, we illustrate the distribution of different green algal 24 lineages in the oceans. The largest group of sequences belongs to the class Mamiellophyceae and in 25 particular to the three genera Micromonas, Bathycoccus and Ostreococcus. These sequences originate 26 mostly from coastal regions. Other groups with a large number of sequences include the 27 Trebouxiophyceae, Chlorophyceae, Chlorodendrophyceae and Pyramimonadales. Some groups, such as 28 the undescribed prasinophytes clades VII and IX, are mostly composed of environmental sequences.
    [Show full text]
  • Estudio Palinológico Y Palinofacies Del Jurásico Medio Y Tardío De La Provincia De Chubut: Sistemática, Bioestratigrafía Y Paleoecología”
    UNIVERSIDAD NACIONAL DEL SUR TESIS DE DOCTOR EN GEOLOGÍA “Estudio palinológico y palinofacies del Jurásico Medio y Tardío de la Provincia de Chubut: Sistemática, Bioestratigrafía y Paleoecología” Lic. Daniela Olivera BAHÍA BLANCA ARGENTINA 2012 Prefacio Esta Tesis se presenta como parte de los requisitos para optar al grado Académico de Doctora en Geología, de la Universidad Nacional del Sur y no ha sido presentada previamente para la obtención de otro título en esta Universidad u otra. La misma contiene los resultados obtenidos en investigaciones llevadas a cabo en el Laboratorio de Palinología dependiente del Departamento de Geología-INGEOSUR, durante el período comprendido entre el 5 de mayo de 2009 y el 17 de agosto de 2012, bajo la dirección de la Dra. Ana María Zavattieri Investigadora Independiente del CONICET y la codirección de la Dra. Mirta Elena Quattrocchio Investigador Superior del CONICET. Daniela Elizabeth Olivera UNIVERSIDAD NACIONAL DEL SUR Secretaría General de Posgrado y Educación Continua La presente tesis ha sido aprobada el .…/.…/.….. , mereciendo la calificación de ......(……………………) I A mis afectos II AGRADECIMIENTOS A mis directoras, Dras. Ana Zavattieri y Mirta Quattrocchio por el apoyo brindado en todo momento, no solo desde lo académico, sino también desde lo personal, incentivándome constantemente a no bajar los brazos y continuar, aún cuando las circunstancias no siempre fueron las ideales. Sobre todo y ante todo, les doy las gracias por creer en mi. Agradezco a la Lic. Lorena Mussotto, compañera de oficina y amiga, con quien hemos transitado este camino, apoyándonos tanto desde lo académico como desde lo afectivo. Le doy las gracias a mi gran amiga y colega la Dra.
    [Show full text]
  • Micractinium Tetrahymenae (Trebouxiophyceae, Chlorophyta), a New Endosymbiont Isolated from Ciliates
    diversity Article Micractinium tetrahymenae (Trebouxiophyceae, Chlorophyta), a New Endosymbiont Isolated from Ciliates Thomas Pröschold 1,*, Gianna Pitsch 2 and Tatyana Darienko 3 1 Research Department for Limnology, Leopold-Franzens-University of Innsbruck, Mondsee, A-5310 Mondsee, Austria 2 Limnological Station, Department of Plant and Microbial Biology, University of Zürich, CH-8802 Kilchberg, Switzerland; [email protected] 3 Albrecht-von-Haller-Institute of Plant Sciences, Experimental Phycology and Culture Collection of Algae, Georg-August-University of Göttingen, D-37073 Göttingen, Germany; [email protected] * Correspondence: [email protected] Received: 28 April 2020; Accepted: 13 May 2020; Published: 15 May 2020 Abstract: Endosymbiosis between coccoid green algae and ciliates are widely distributed and occur in various phylogenetic lineages among the Ciliophora. Most mixotrophic ciliates live in symbiosis with different species and genera of the so-called Chlorella clade (Trebouxiophyceae). The mixotrophic ciliates can be differentiated into two groups: (i) obligate, which always live in symbiosis with such green algae and are rarely algae-free and (ii) facultative, which formed under certain circumstances such as in anoxic environments an association with algae. A case of the facultative endosymbiosis is found in the recently described species of Tetrahymena, T. utriculariae, which lives in the bladder traps of the carnivorous aquatic plant Utricularia reflexa. The green endosymbiont of this ciliate belonged to the genus Micractinium. We characterized the isolated algal strain using an integrative approach and compared it to all described species of this genus. The phylogenetic analyses using complex evolutionary secondary structure-based models revealed that this endosymbiont represents a new species of Micractinium, M.
    [Show full text]