DOCSLIB.ORG

Contaminação Bacteriana E Fúngica Dos Tubos Endotraqueais E Traqueias Respiratórias

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Contaminação Bacteriana E Fúngica Dos Tubos Endotraqueais E Traqueias Respiratórias ANA FERNANDES MORENO FERREIRA DE LEMOS Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias Orientador: Professor Doutor Pedro Faísca Universidade Lusófona de Humanidades e Tecnologias Faculdade de Medicina Veterinária Lisboa 2016 ANA FERNANDES MORENO FERREIRA DE LEMOS - Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias ANA FERNANDES MORENO FERREIRA DE LEMOS Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias Dissertação defendida em provas públicas para a obtenção do Grau de Mestre em Medicina Veterinária no curso de Mestrado Integrado em Medicina Veterinária conferido pela Universidade Lusófona de Humanidades e Tecnologias no dia 31 de Outubro com a seuinte composição de Júri: Presidente: Professor Doutor João Requicha Arguente: Professora Doutora Margarida Simões Orientador: Prodessor Doutor Pedro Faísca Orientador: Professor Doutor Pedro Faísca Co-Orientador: Dr. António Martinho Universidade Lusófona de Humanidades e Tecnologias Faculdade de Medicina Veterinária Lisboa 2016 Universidade Lusófona de Humanidades e Tecnologias,Faculdade de Medicina Veterinária2 ANA FERNANDES MORENO FERREIRA DE LEMOS - Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias Dedicatória À minha família que sempre acreditou em mim. Universidade Lusófona de Humanidades e Tecnologias,Faculdade de Medicina Veterinária3 ANA FERNANDES MORENO FERREIRA DE LEMOS - Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias Agradecimentos No âmbito da realização da dissertação gostaria de agradecer ao professor Dr. António Martinho pela paciência, dedicação e amizade, não só durante este período, mas também ao longo destes 6 anos. Aos Professores Doutores Nuno Cardoso e Rui Onça por todos os conhecimentos transmitidos, ajuda, disponibilidade e boa disposição. À Dra. Mariana Garcez pelo apoio fundamental e orientação em toda a parte experimental deste trabalho. Ao Professor Doutor Pedro Faísca, ao Dr. Mauro Bragança e às adoradas Sra. Isabel e Sra. Manuela que me apoiaram na parte logística deste estudo. A todos os professores com quem tive oportunidade de aprender a ser médica veterinária e a toda a equipa do Hospital Veterinário do Restelo, em especial ao Dr. Jorge Cid, um muito obrigada por tão bem me receberem como estagiária, por todos os conhecimentos transmitidos e pelo óptimo convívio que proporcionaram. Um agradecimento especial à minha avó Maria de Lurdes, pelo carinho e orações em dias de exame e a todos os Morenos, os Ferreira de Lemos e os Dias. Aos meus pais, Maria de Lurdes e Paulo, que me incentivaram a seguir este sonho e o tornaram realidade e aos meus irmãos, Beatriz, Ricardo e Paulo, pela paciência, amizade, ajuda, compreensão nos meus momentos mais difíceis e por me cederem o “espaço de estudo”. Ao Ricardo por ter estado sempre ao meu lado com muita paciência, por me ter dado força e porque sem ele seria impossível este sonho se ter concretizado. A todos os meus amigos, incluindo os meus grandes companheiros de curso, Mariana Pereira Coutinho, Sara Reis, Rita Castro, Margarida Almeida, Mónica Silvério, Joana Dias e Mariana Correia por terem feito parte desta etapa da minha vida e por terem tornado estes 6 anos bastante mais fáceis. Universidade Lusófona de Humanidades e Tecnologias,Faculdade de Medicina Veterinária4 ANA FERNANDES MORENO FERREIRA DE LEMOS - Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias Resumo Introdução: A utilização do tubo endotraqueal (TE) e da traqueia respiratória (TR) é fundamental na prática cirúrgica veterinária e a reutilização dos mesmos é também uma prática recorrente de forma a obter uma diminuição de custos. A avaliação da contaminação microbiológica dos mesmos está amplamente documentada em Medicina Humana. O mesmo não se sucede em Medicina Veterinária, pois devido aos altos custos de manutenção, torna-se impraticável realizar estudos microbiológicos de rotina. Material e Métodos: O presente estudo foi realizado num Hospital de Medicina Veterinária na zona de Lisboa, com o objectivo de determinar a contaminação bacteriana e fúngica nestes equipamentos. Procedeu-se à avaliação clínica e radiográfica de todos os pacientes. Foram recolhidas amostras microbiológicas a partir de 30 TEs e 30 TRs reutilizados, escolhidos aleatoriamente, antes do procedimento cirúrgico e cultivadas em meios de cultura para bactérias e fungos. Este procedimento foi realizado independentemente do sexo, raça e idade do animal, tipo e tempo de cirurgia. Seguido de determinação, caracterização e identificação microbiológica. Resultados: Verificou-se crescimento bacteriano e/ou fúngico total de 73% (44 amostras positivas em 60 amostras). Por fim, correlacionaram-se os resultados com o aparecimento de afecções respiratórias. Conclusão: O estudo de fontes de contaminação em ambientes hospitalares veterinários é uma actividade em crescimento, sendo necessário o desenvolvimento de programas de controlo mais específicos e eficazes, que tenham como pilares básicos um sistema de vigilância efectivo combinado com uma rotina de análises microbiológicas. Palavras-chave: Contaminação microbiológica, tubos endotraqueais, traqueias respiratórias. Universidade Lusófona de Humanidades e Tecnologias,Faculdade de Medicina Veterinária5 ANA FERNANDES MORENO FERREIRA DE LEMOS - Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias Abstract Background: The use of the endotracheal tube (ET) and the tubing of breathing circuit systems (tBCS) is fundamental for the practice of the veterinary surgery and reusing them is also a recurring way of reducing costs. The evaluation of the microbiological contamination is widely documented in Human Medicine. In Veterinary Medicine however, due to high maintenance costs, it is impossible to perform routine microbiological studies. Materials and methods: The current study was done in a Veterinary Medicine Hospital in the Lisbon area, with the objective of defining the bacteriological and fungi contamination of these medical devices. All patients underwent a clinical and radiographic evaluation. Microbiological samples were taken from 30 ET and 30 tBCS reused randomly chosen and were collected before initiating the surgical procedure and cultured for bacteria and fungi. This procedure was performed regardless of the sex, race and age of the animal, type and time of surgery. Followed by determination, characterization and microbiological identification. Results: Bacterial and/or fungal growth was found to be 73% (44 positive samples in 60 samples). In the end the results were correlated with the appearance of respiratory disorders. Conclusion: The study of the sources of contamination in veterinary hospital environments is a growing activity, creating the need to develop more specific and efficient control programs, which will be supported by a basis of a surveillance system combined with a routine of microbiological analysis. Key-words: Microbiological contamination, endotracheal tubes, tubing of breathing circuit systems. Universidade Lusófona de Humanidades e Tecnologias,Faculdade de Medicina Veterinária6 ANA FERNANDES MORENO FERREIRA DE LEMOS - Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias Lista de Abreviaturas e Símbolos % – Percentagem ® – Marca registada > – Maior ASA – American Society of Anesthesiologists ATS – American Thoracic Society BALT – Bronchus-associated lymphoid tissue BCMH – British Columbia Ministry of Health Bpm – Batimentos por minuto CDC – Centers for Disease Control and Prevention CFSPH – Centers for Food Security & Public Health CHRISP – Centre for Healthcare Related Infection Surveillance and Prevention CO2 – Dióxido de Carbono CRA – Circuito Respiratório Anestésico CRAs – Circuitos Respiratórios Anestésicos CSC – Columbia + 5% de Sangue de Carneiro DMUU– Dispositivos Médicos de Uso Único E. coli – Escherichia coli E. coli ExPEcC – e. coli extraintestinal ET – Endotracheal Tube FDA – Food and Drug Administration Ig – Imunoglobulina IICAB – Institute for International Cooperation in Animal Biologics MRSA – Methicillin-Resistant Staphilococcus aureus (Staphylococcus aureus Resistente à Meticilina) NaCl – Cloreto de sódio O2 – Oxigénio ºC – Graus Celsius OE – Óxido de Etileno OVH – Ováriohisterectomia PAM – Pulmonary Alveolar Macrophages Universidade Lusófona de Humanidades e Tecnologias,Faculdade de Medicina Veterinária7 ANA FERNANDES MORENO FERREIRA DE LEMOS - Contaminação Bacteriana e Fúngica dos Tubos Endotraqueais e Traqueias Respiratórias PAV – Pneumonia Associada à Ventilação PNCI – Programa Nacional de Controlo de Infecção PVC – Policloreto de vinil QMDA – Committee on Quality Management and Departmental Administration Rpm – Respirações por minuto S. aureus – Staphylococcus aureus S. epidermidis – Staphylococcus epidermidis S. pseudointermedius – Staphylococcus pseudointermedius S. saprophyticus – Staphylococcus saprophyticus SCENIHR – Scientific Committee on Emerging and Newly Identified Health Risks SRD – Sem Raça Definida SVA – Swedish Veterinary Association TAV– Traqueobronquite Associada à Ventilação tBCS – tubing of Breathing Circuit System TE – Tubo Endotraqueal TEs – Tubos Endotraqueais TR – Traqueia Respiratória TRs – Traqueias Respiratórias UTI – Urinay tract infection α – Alfa β – Beta γ – Gama µm – Micrómetro Índice Índice de Figuras ................................................................................................................................
Load more

Recommended publications
  • Phylogeny of Chrysosporia Infecting Reptiles: Proposal of the New Family Nannizziopsiaceae and Five New Species
    CORE Metadata, citation and similar papers at core.ac.uk Provided byPersoonia Diposit Digital 31, de Documents2013: 86–100 de la UAB www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158513X669698 Phylogeny of chrysosporia infecting reptiles: proposal of the new family Nannizziopsiaceae and five new species A.M. Stchigel1, D.A. Sutton2, J.F. Cano-Lira1, F.J. Cabañes3, L. Abarca3, K. Tintelnot4, B.L. Wickes5, D. García1, J. Guarro1 Key words Abstract We have performed a phenotypic and phylogenetic study of a set of fungi, mostly of veterinary origin, morphologically similar to the Chrysosporium asexual morph of Nannizziopsis vriesii (Onygenales, Eurotiomycetidae, animal infections Eurotiomycetes, Ascomycota). The analysis of sequences of the D1-D2 domains of the 28S rDNA, including rep- ascomycetes resentatives of the different families of the Onygenales, revealed that N. vriesii and relatives form a distinct lineage Chrysosporium within that order, which is proposed as the new family Nannizziopsiaceae. The members of this family show the mycoses particular characteristic of causing skin infections in reptiles and producing hyaline, thin- and smooth-walled, small, Nannizziopsiaceae mostly sessile 1-celled conidia and colonies with a pungent skunk-like odour. The phenotypic and multigene study Nannizziopsis results, based on ribosomal ITS region, actin and β-tubulin sequences, demonstrated that some of the fungi included Onygenales in this study were different from the known species of Nannizziopsis and Chrysosporium and are described here as reptiles new. They are N. chlamydospora, N. draconii, N. arthrosporioides, N. pluriseptata and Chrysosporium longisporum. Nannizziopsis chlamydospora is distinguished by producing chlamydospores and by its ability to grow at 5 °C.
    [Show full text]
  • New Xerophilic Species of Penicillium from Soil
    Journal of Fungi Article New Xerophilic Species of Penicillium from Soil Ernesto Rodríguez-Andrade, Alberto M. Stchigel * and José F. Cano-Lira Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV), Sant Llorenç 21, Reus, 43201 Tarragona, Spain; [email protected] (E.R.-A.); [email protected] (J.F.C.-L.) * Correspondence: [email protected]; Tel.: +34-977-75-9341 Abstract: Soil is one of the main reservoirs of fungi. The aim of this study was to study the richness of ascomycetes in a set of soil samples from Mexico and Spain. Fungi were isolated after 2% w/v phenol treatment of samples. In that way, several strains of the genus Penicillium were recovered. A phylogenetic analysis based on internal transcribed spacer (ITS), beta-tubulin (BenA), calmodulin (CaM), and RNA polymerase II subunit 2 gene (rpb2) sequences showed that four of these strains had not been described before. Penicillium melanosporum produces monoverticillate conidiophores and brownish conidia covered by an ornate brown sheath. Penicillium michoacanense and Penicillium siccitolerans produce sclerotia, and their asexual morph is similar to species in the section Aspergilloides (despite all of them pertaining to section Lanata-Divaricata). P. michoacanense differs from P. siccitol- erans in having thick-walled peridial cells (thin-walled in P. siccitolerans). Penicillium sexuale differs from Penicillium cryptum in the section Crypta because it does not produce an asexual morph. Its ascostromata have a peridium composed of thick-walled polygonal cells, and its ascospores are broadly lenticular with two equatorial ridges widely separated by a furrow. All four new species are xerophilic.
    [Show full text]
  • S21 Konferans TIPTA ÖNEMLİ MANTARLARIN FİLOGENETİK VE
    Konferans TIPTA ÖNEMLİ MANTARLARIN FİLOGENETİK VE SİSTEMATİĞİ Ahmet ASAN Trakya Üniversitesi, Fen-Edebiyat Fakültesi Biyoloji Bölümü, Edirne, ([email protected]; [email protected]) Fungal infeksiyonlar dünyada oldukça yaygındır. Bulaşıcı özellik taşıyan yüzeyel infeksiyon- lar, dermatofitler ve Candida türleri tarafından oluşturulmaktadır. Bu tür infeksiyonların tanısı zordur ve bazen ekzama gibi diğer hastalıklarla karıştırılabilmektedir (1). Mantarların insanlarda hastalığa yol açtığı, ilk defa 1839’da gösterilmiştir. Schoenlein ve Gruby, daha sonra Trichophyton schoenleinii olarak adlandırılan ve kafa derisinde infeksiyona veya kelliğe (= favus) neden olan türü keşfetmişlerdir (2). Fakat yıllar boyunca fungal hastalıklar bakteriyal hastalıkların gölgesinde kalmıştır. Mantar hastalıklarının önemi, özellikle 1970’li yıllardan sonra artmıştır. Çünkü bu tarihden sonra, uluslararası ulaşım ve bağışıklığı bastırıcı ilaçların kullanımı artmış; ilave olarak AIDS hastalığı ortaya çıkmıştır. Bilinmeyen veya başlangıçta patojen olmadığı düşünülen mantarların neden olduğu fırsatçı infeksiyonların gittikçe arttığı da bilinmektedir. İnsanlarda infeksiyona neden olan birçok tür ve birçok yeni insan patojeni her yıl keşfedilmekte, bu durum fungal taksonomiyi önemli hale getirmektedir (3). Doğru tedavi için mantarın doğru tanınması çok önemlidir. Gelişen dünyada bağışıklık sistemi zayıf hastalarda meydana gelen ölümlerin % 5’den fazlasının sebebi patojen mantarlardır. Tıbbi tedavide artış görülmesine rağmen, fungal infeksiyonların insidansında
    [Show full text]
  • Comparative Genomic Analyses of the Human Fungal Pathogens Coccidioides and Their Relatives
    Downloaded from genome.cshlp.org on September 28, 2021 - Published by Cold Spring Harbor Laboratory Press Letter Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives Thomas J. Sharpton,1,11 Jason E. Stajich,1 Steven D. Rounsley,2 Malcolm J. Gardner,3 Jennifer R. Wortman,4 Vinita S. Jordar,5 Rama Maiti,5 Chinnappa D. Kodira,6 Daniel E. Neafsey,6 Qiandong Zeng,6 Chiung-Yu Hung,7 Cody McMahan,7 Anna Muszewska,8 Marcin Grynberg,8 M. Alejandra Mandel,2 Ellen M. Kellner,2 Bridget M. Barker,2 John N. Galgiani,9 Marc J. Orbach,2 Theo N. Kirkland,10 Garry T. Cole,7 Matthew R. Henn,6 Bruce W. Birren,6 and John W. Taylor1 1Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California 94720, USA; 2Department of Plant Sciences, The University of Arizona, Tucson Arizona 85721, USA; 3Department of Global Health, Seattle Biomedical Research Institute, Seattle, Washington 98109-5219, USA; 4Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA; 5J. Craig Venter Institute, Rockville, Maryland 20850, USA; 6Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; 7Department of Biology, The University of Texas at San Antonio, San Antonio, Texas 78249, USA; 8Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw 02-106, Poland; 9Valley Fever Center for Excellence, The University of Arizona, Tuscon, Arizona 85721, USA ; 10Department of Pathology, University of California at San Diego, La Jolla, California 92093, USA While most Ascomycetes tend to associate principally with plants, the dimorphic fungi Coccidioides immitis and Coccidioides posadasii are primary pathogens of immunocompetent mammals, including humans.
    [Show full text]
  • Molecular Detection of Human Fungal Pathogens As (Except a Few That Have Newly Evolved As Anthropophilic Dermatophytes
    36 Microsporum* Rahul Sharma and Yvonne Gräser Contents 36.1 . Introduction...................................................................................................................................................................... 285 36.1.1. Classification,.Morphology,.Biology,.and.Epidemiology..................................................................................... 287 36.1.1.1. Classification..........................................................................................................................................287 36.1.1.2. Morphology............................................................................................................................................288 36.1.1.3. Biology.and.Epidemiology.....................................................................................................................289 36.1.2. Clinical.Features.and.Pathogenesis......................................................................................................................290 36.1.2.1. Clinical.Features....................................................................................................................................290 36.1.2.2. Pathogenesis...........................................................................................................................................290 36.1.3. Diagnosis.............................................................................................................................................................. 291 36.1.3.1.
    [Show full text]
  • Metagenome Sequence of Elaphomyces Granulatus From
    bs_bs_banner Environmental Microbiology (2015) 17(8), 2952–2968 doi:10.1111/1462-2920.12840 Metagenome sequence of Elaphomyces granulatus from sporocarp tissue reveals Ascomycota ectomycorrhizal fingerprints of genome expansion and a Proteobacteria-rich microbiome C. Alisha Quandt,1*† Annegret Kohler,2 the sequencing of sporocarp tissue, this study has Cedar N. Hesse,3 Thomas J. Sharpton,4,5 provided insights into Elaphomyces phylogenetics, Francis Martin2 and Joseph W. Spatafora1 genomics, metagenomics and the evolution of the Departments of 1Botany and Plant Pathology, ectomycorrhizal association. 4Microbiology and 5Statistics, Oregon State University, Corvallis, OR 97331, USA. Introduction 2Institut National de la Recherché Agronomique, Centre Elaphomyces Nees (Elaphomycetaceae, Eurotiales) is an de Nancy, Champenoux, France. ectomycorrhizal genus of fungi with broad host associa- 3Bioscience Division, Los Alamos National Laboratory, tions that include both angiosperms and gymnosperms Los Alamos, NM, USA. (Trappe, 1979). As the only family to include mycorrhizal taxa within class Eurotiomycetes, Elaphomycetaceae Summary represents one of the few independent origins of the mycorrhizal symbiosis in Ascomycota (Tedersoo et al., Many obligate symbiotic fungi are difficult to maintain 2010). Other ectomycorrhizal Ascomycota include several in culture, and there is a growing need for alternative genera within Pezizomycetes (e.g. Tuber, Otidea, etc.) approaches to obtaining tissue and subsequent and Cenococcum in Dothideomycetes (Tedersoo et al., genomic assemblies from such species. In this 2006; 2010). The only other genome sequence pub- study, the genome of Elaphomyces granulatus was lished from an ectomycorrhizal ascomycete is Tuber sequenced from sporocarp tissue. The genome melanosporum (Pezizales, Pezizomycetes), the black assembly remains on many contigs, but gene space perigord truffle (Martin et al., 2010).
    [Show full text]
  • Virulence Evolution of Fungal Pathogens in Social and Solitary Bees with an Emphasis on Multiple Infections
    Utah State University DigitalCommons@USU All Graduate Theses and Dissertations Graduate Studies 8-2015 Virulence Evolution of Fungal Pathogens in Social and Solitary Bees with an Emphasis on Multiple Infections Ellen G. Klinger Utah State University Follow this and additional works at: https://digitalcommons.usu.edu/etd Part of the Biology Commons Recommended Citation Klinger, Ellen G., "Virulence Evolution of Fungal Pathogens in Social and Solitary Bees with an Emphasis on Multiple Infections" (2015). All Graduate Theses and Dissertations. 4441. https://digitalcommons.usu.edu/etd/4441 This Dissertation is brought to you for free and open access by the Graduate Studies at DigitalCommons@USU. It has been accepted for inclusion in All Graduate Theses and Dissertations by an authorized administrator of DigitalCommons@USU. For more information, please contact [email protected]. VIRULENCE EVOLUTION OF FUNGAL PATHOGENS IN SOCIAL AND SOLITARY BEES WITH AN EMPHASIS ON MULTIPLE INFECTIONS by Ellen G. Klinger A dissertation submitted in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in Biology Approved: Dennis L. Welker Rosalind R. James Major Professor Project Advisor Donald W. Roberts John R. Stevens Committee Member Committee Member Carol D. von Dohlen Mark R. McLellan Committee Member Vice President for Research and Dean of the School of Graduate Studies UTAH STATE UNIVERSITY Logan, Utah 2015 ii Disclaimer regarding products used in the dissertation: This paper reports the results of research only. The mention of a proprietary product does not constitute an endorsement or a recommendation by the USDA for its use. Disclaimer about copyrights: The dissertation was prepared by a USDA employee as part of his/her official duties.
    [Show full text]
  • Biological and Evolutionary Diversity in the Genus Aspergillus
    Sexual structures in Aspergillus -- morphology, importance and genomics David M. Geiser Department of Plant Pathology Penn State University University Park, PA Geiser mini-CV • 1989-95: PhD at University of Georgia (Bill Timberlake and Mike Arnold): Aspergillus molecular evolutionary genetics (A. nidulans) • 1995-98: postdoc at UC Berkeley (John Taylor): (A. flavus/oryzae/parasiticus, A. fumigatus, A. sydowii) • 1998-: Faculty at Penn State; Director of Fusarium Research Center -- molecular evolution of Fusarium and other fungi Chaetosartorya Petromyces Hemicarpenteles Neosartorya Fennellia Aspergillus Neocarpenteles Eurotium Warcupiella Neopetromyces Emericella Sexual structures in Aspergillus -- morphology, importance and genomics • Sexual stages associated with Aspergillus • The impact (and lack thereof) of the sexual stage on population biology • What does it mean? Characteristics of clinically important Aspergillus spp. • Ability to grow at 37C • Commonly encountered by humans • Prolific sporulators • Nothing here about sexual stages Approx. 1/3 Aspergillus species has a known sexual stage Petromyces (3) Neopetromyces (1) Neosartorya (32, 3 heterothallic) Chaetosartorya (4) Aspergillus Emericella (34, 1 heterothallic) 148 homothallic 4 heterothallic (427 names) Fennellia (3) Eurotium (69) Warcupiella (1) Hemicarpenteles (4) Neocarpenteles (1) Heterothallics rare; virtually all have a conidial stage Types of ascomata cleistothecium (no hymenium - naked passive spore dispersal) asci asci and paraphyses (hymenium) apothecium perithecium
    [Show full text]
  • Studies on Microbial Production of Lipoxygenase Inhibitor
    STUDIES ON MICROBIAL PRODUCTION OF LIPOXYGENASE INHIBITOR A thesis submitted to the UNIVERSITY OF MYSORE for the award of the degree of DOCTOR OF PHILOSOPHY IN BIOTECHNOLOGY By CHIDANANDA.C Department of Fermentation Technology and Bioengineering Central Food Technological Research Institute Council of Scientific and Industrial Research Mysore-570020, INDAI June 2008 Chidananda. C, Date: Senior Research Fellow, Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore-570 013. DECLARATION I hereby declare that the thesis entitled “STUDIES ON MICROBIAL PRODUCTION OF LIPOXYGENASE INHIBITOR” submitted to the University of Mysore for the award of the degree of DOCTOR OF PHILOSOPHY is the result of the research work carried out by me in the Discipline of Fermentation Technology and Bioengineering, Central Food Technological Research Institute, Mysore, India, under the guidance of Dr Avinash P Sattur during the period April 2005- June 2008. I further declare that the work embodied in this thesis had not been submitted for the award of degree, diploma or any other similar title. (Chidananda. C) Dr. Avinash P. Sattur, Date: Scientist, Fermentation Technology and Bioengineering Department, CERTIFICATE I hereby certify that the thesis entitled “STUDIES ON MICROBIAL PRODUCTION OF LIPOXIGENASE INHIBITOR” submitted to the University of Mysore for the award of the degree of DOCTOR OF PHILOSOPHY by Mr. CHIDANANDA.C, is the result of the research work carried out by him in the discipline of Fermentation Technology and Bioengineering, Central Food Technological Research Institute, Mysore, India, under my guidance during the period April 2005-June 2008. (Avinash P Sattur) Abstract The thesis reports isolation of several fungal cultures from forest soil and screening of the metabolites for their ability to produce inhibitors against lipoxygenase.
    [Show full text]
  • First Detection of the Larval Chalkbrood Disease Pathogen Ascosphaera Apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in Adult Bumble Bees
    First Detection of the Larval Chalkbrood Disease Pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in Adult Bumble Bees Maxfield-Taylor, S.A., Mujic, A.B., Rao, S. (2015) First Detection of the Larval Chalkbrood Disease Pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in Adult Bumble Bees. PLoS ONE 10(4). doi:10.1371/journal.pone.0124868 10.1371/journal.pone.0124868 Public Library of Science Version of Record http://cdss.library.oregonstate.edu/sa-termsofuse RESEARCH ARTICLE First Detection of the Larval Chalkbrood Disease Pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in Adult Bumble Bees Sarah A. Maxfield-Taylor1, Alija B. Mujic2, Sujaya Rao1* 1 Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon, United States of America, 2 Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, United States of America * [email protected] Abstract OPEN ACCESS Fungi in the genus Ascosphaera (Ascomycota: Eurotiomycetes: Ascosphaerales) cause Citation: Maxfield-Taylor SA, Mujic AB, Rao S (2015) chalkbrood disease in larvae of bees. Here, we report the first-ever detection of the fungus First Detection of the Larval Chalkbrood Disease in adult bumble bees that were raised in captivity for studies on colony development. Wild Pathogen Ascosphaera apis (Ascomycota: queens of Bombus griseocollis, B. nevadensis and B. vosnesenskii were collected and Eurotiomycetes: Ascosphaerales) in Adult Bumble maintained for establishment of nests. Queens that died during rearing or that did not lay Bees. PLoS ONE 10(4): e0124868. doi:10.1371/ journal.pone.0124868 eggs within one month of capture were dissected, and tissues were examined microscopi- cally for the presence of pathogens.
    [Show full text]
  • End-40-75 (Endode-Endope) Mohammed AL- Hamdany
    الموسوعة العربية ﻷمراض النبات والفطريات Arabic Encyclopedia of Plant Pathology &Fungi إعداد الدكتور محمد عبد الخالق الحمداني Mohammed AL- Hamdany End-40-75 (Endode-Endope) Contents Codes Page No. Table of contents 1 Endod… Endodermophyton End-40 2 Endodesmia (Broomeola) End-41 4 Endodesmidium Canter 1949 End-42 5 Endodothella ( Phyllachora ) End-43 6 Endodothiora Petr., 1929. End-44 19 Endodromia ( Echinostelium) End-45 21 Endog Endogenospora R.F. Castañeda, O. Morillo & Minter, 2010. End-46 22 Endogenous Inoculum End-47 24 Endogloea (Phomopsis) End-48 25 Endogonaceae End-49 34 Endogonales End-50 35 Endogone Link, 1809. End-51 36 Endogonella (Claziella) End-52 39 Endogonomycetes End-53 41 Endogonopsidaceae End-54 41 Endogonopsis R. Heim, 1966. End-55 42 Endoh… Endohormidium ( Corynelia) End-56 43 Endohyalina Marbach, 2000. End-57 46 Endol… Endolepiotula Singer, 1963. End-58 49 Endolpidium (Olipdium ) End-59 52 Endom…. 1 Endomelanconiopsidaceae End-60 55 Endomelanconiopsis E.I. Rojas & Samuels,2008 End-61 56 Endomelanconium Petr., 1940. End-62 58 Endomeliola S. Hughes & Piroz.,. 1994. End-63 60 Endomyces Reess, Bot. 1870 End-64 62 Endomycetaceae End-65 64 Endomycetales End-66 66 Endomycodes Delitsch, 1943 End-67 67 Endomycopsella End-68 68 Endomycopsis (Saccharomycopsis) End-69 70 Endomycorrhizae End-70 73 Endon… Endonema ( Pascherinema) End-71 75 Endonevrum (Mycenastrum) End-72 77 Endopa-Endope Endoparasites End-73 79 Endoparasitic Nematodes End-74 80 Endoperplexa P. Roberts,1993 End-75 82 References 85 End-40. الجنس الكيسي المختلف عليهEndodermophyton أقرت قانونية إسم الجنس الكيسي Endodermophyton Castell., 1910 وأنواعه الثمانية بضمنها النوع اﻷصلي Endodermophyton castellanii (Perry) Castell.
    [Show full text]
  • A Rock-Inhabiting Ancestor for Mutualistic and Pathogen-Rich Fungal Lineages
    available online at www.studiesinmycology.org STUDIE S IN MYCOLOGY 61: 111–119. 2008. doi:10.3114/sim.2008.61.11 A rock-inhabiting ancestor for mutualistic and pathogen-rich fungal lineages C. Gueidan1,3*, C. Ruibal Villaseñor2,3, G. S. de Hoog3, A. A. Gorbushina4,5, W. A. Untereiner6 and F. Lutzoni1 1Department of Biology, Duke University, Box 90338, Durham NC, 27708 U.S.A.; 2Departamento de Ingeniería y Ciencia de los Materiales, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid (UPM), José Gutiérrez Abascal 2, 28006 Madrid, Spain; 3CBS Fungal Biodiversity Centre, P.O. Box 85167, NL-3508 AD Utrecht, The Netherlands; 4Geomicrobiology, ICBM, Carl von Ossietzky Universität, P.O. Box 2503, 26111 Oldenburg, Germany; 5LBMPS, Department of Plant Biology, Université de Genève, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland; 6Department of Biology, Brandon University, Brandon, MB Canada R7A 6A9 Correspondence: Cécile Gueidan, [email protected] Abstract: Rock surfaces are unique terrestrial habitats in which rapid changes in the intensity of radiation, temperature, water supply and nutrient availability challenge the survival of microbes. A specialised, but diverse group of free-living, melanised fungi are amongst the persistent settlers of bare rocks. Multigene phylogenetic analyses were used to study relationships of ascomycetes from a variety of substrates, with a dataset including a broad sampling of rock dwellers from different geographical locations. Rock- inhabiting fungi appear particularly diverse in the early diverging lineages of the orders Chaetothyriales and Verrucariales. Although these orders share a most recent common ancestor, their lifestyles are strikingly different. Verrucariales are mostly lichen-forming fungi, while Chaetothyriales, by contrast, are best known as opportunistic pathogens of vertebrates (e.g.
    [Show full text]