Submission Form Genetic tests for D-45659 Recklinghausen Berghäuser Straße 295 (Please write legibly and in block letters) Phone: +49 [0] 2361 3000 121 Fax: +49 [0] 2361 3000 169 Membership breed club: YES ☐ NO ☐ E-mail: [email protected] (If YES - please attach a copy of your membership card) Internet: www.biofocus.de

Owner/Sender Full name: ______Country:______Street:______Phone:______City:______Fax:______State:______Zip-Code:______E-mail:______Date/Signature:______Dog information Name: ______Tattoo-No.: ______Breed: ______Registration-No.: ______Coat color: ______Chip-No.: ______(required for certificates) Date of birth: ______Gender: female ☐ male ☐ ’s parents: (only necessary for coat color and coat type tests) Dam’s name: ______Sire’s name: ______Breed: ______Breed: ______Coat color: ______Coat color: ______

Coat length longhair ☐ shorthair ☐ Coat length longhair ☐ shorthair ☐ (only necessary for coat length test) (only necessary for coat length test) Sample specification Sampling by: Owner/Sender ☐ Independent authority (e.g. veterinarian or breed warden) ☐ Sample material: buccal swabs (2 samples) ☐ EDTA-blood (approx. 0.5 ml) ☐ Please perform the buccal swab thoroughly and make sure that the dog being tested has not eaten anything for at least 30 minutes. Further information about sample collection is available on our homepage. Sample labeling: ______Date of sampling: ______I hereby confirm that the identity of the above-listed animal was verified and that the samples derive from the above-listed animal. ______Stamp and signature independent authority Independent authority name in block letters:______Submission of results Test-results will be send to either the Owner/Sender and/or Veterinarian Test-results to: Owner/Sender ☐ Veterinarian ☐ Owner/Sender and Veterinarian ☐ Invoice to: Owner/Sender ☐ Veterinarian ☐ Certificate: ☐ certificates will be provided for each test separately. Certificate for the first test is free of charge, certificates for two to five tests will cost 7.14 €, more than five certificates will cost 14.28 € (incl. VAT). Certificates can only be issued if Chip-no. are provided. It is recommended that sampling is carried out by an independent authority (e.g. veterinarian or breed warden) to confirm sample origin. Data privacy statement: The personal data collected in this form is used to perform the commissioned analysis. Based on art. 6 (1b) EU-DSGVO data will only be used for processing and fulfillment of the contract as well as to process the payment transaction. Biofocus LADR data protection official is Dirk Fox, Secorvo Security Consultant GmbH, Ettlinger Str. 12-14, 76137 Karlsruhe (email: [email protected]). Information about your personal data stored by Biofocus LADR can be inquired in written form by contacting Manuel Ostendorff (email: [email protected]). Owner/Sender: ______Dog’s name: ______Genetic Disorders Actual prices are listed on our homepage and can be inquired by e-mail. Please pay attention to our test-packages described at the end of the form.

Ataxia, cerebellar, ATP1B2-related (SDCA2) Haemophilia A* ( Dog) ☐ X-linked ☐ Ataxia, cerebellar, KCNJ10-related (SDCA1) ☐ (Havanese) (Belgian Shepherd Dog) Hyperkeratosis, palmoplantar (HFH) ☐ Bandera's neonatal ataxia ☐ (Irish , Kromfohrländer) (Coton de Tulear) Hyperuricosuria (HU) ☐ Bleeding disorder, P2RY12-related (all breeds) (Great Swiss Mountain Dog) ☐ Ichthyosis (Icht-GR) Brachyury / Tail, short () ☐ (, Austrian Pinscher, Australian Stumpy Tail Cattle Dog, ☐ Krabbe disease Bourbonnais Pointing Dog, Brazilian Terrier, Brittany Spaniel, Croatian (, Irish Red Setter, West Highland White Terrier) ☐ Shepherd Dog, Danish-Swedish Farmdog, , Karelian Bear Dog, , , , Savoy Sheepdog, Leukocyte adhesion deficiency, type I (CLAD) ☐ , , ) (Irish Red Setter, Irish Red and White Setter) Chondrodysplasia Malignant hyperthermia (MH) FGF4-Insertion-CFA18 ☐ (all breeds) ☐ (Basset , Cairn Terrier, Dachshund, , Mucopolysaccharidosis VII (MPS VII) Lancashire Heeler, , Petit Basset Griffon Vendeen, Skye ( Dog) ☐ Terrier, Swedish Vallhund, Tebetian Spaniel, (Cardigan), Welsh Corgi (Pembroke), West Highland White Terrier) Multidrug resistance 1 (MDR1)* ☐ Chondrodysplasia / (Australian Shepherd, Bobtail (), Border , Collie ☐ (rough), Collie (smooth), German Shepherd Dog, Longhaired , Intervertebral Disc Disease Type 1 (IVDD) , Silken Windhound, Toy Australian Shepherd, White FGF4-Insertion-CFA12 Swiss Shepherd Dog, Wäller) (breeds with short and long leg phenotypes) Muscular hypertrophy (bully whippet) Chondrodysplasia / ☐ ☐ (Whippet) Intervertebral Disc Disease Type 1 (IVDD) Musladin-Lueke syndrome (MLS) FGF4-Insertion-CFA12 and CFA18 () ☐ (breeds with short and long leg phenotypes) Myoclonus epilepsy of Lafora* Copper toxicosis / Wilson disease ☐ min. 14 days, blood sample required ☐ ( ) (, Beagle, Dachshund (wire-haired)) Cystinuria, type I - A ☐ Myopathy, centronuclear (HMLR) (Landseer, Newfoundland) () ☐ Cystinuria, type III Narcolepsy SLC3A1 and SLC7A9 mutations ☐ (Dachshund, Dobermann, Labrador Retriever) ☐ (American , Bulldog, Continental Bulldog, French Bulldog, Leavitt Bulldog, Olde English Bulldog) Neonatal cerebellar cortical degeneration (NCCD) ☐ Degenerative myelopathy (DM Combi-Test 1) (Beagle) SOD1:c.118G>A mutation Exon 2* / SOD1: c.166+9_c.166+10ins ☐ Neonatal encephalopathy with seizures (NEWS) (Collie (rough), Hovawart, Poodle) (Poodle) ☐ Degenerative myelopathy (DM Combi-Test 2) Nephropathy SOD1:c.52A>T mutation Exon 1 / SOD1:c.118G>A mutation Exon 2* ☐ () ☐ (Bernese Mountain Dog, Great Swiss Mountain Dog) Neuroaxonal dystrophy (NAD) Degenerative myelopathy (DM) (Papillon, Spanish Water Dog) ☐ SOD1:c.52A>T mutation Exon 1 ☐ (Bernese Mountain Dog, Great Swiss Mountain Dog) Neuronal ceroid lipofuscinosis (NCL) (American Bulldog, American Staffordshire Terrier, , ☐ Degenerative myelopathy (DM)* ☐ Australien Sheperd, , Chihuahua, Chinese Crested, Dachshund SOD1:c.118G>A mutation Exon 2* (long-haired), , Tibet Terrier) (all breeds) Neutropenia, cyclic / Grey Collie Syndrom Dry Eye Curly Coat + Episodic Falling (Combi)* (Collie (rough), Collie (smooth)) ☐ (Cavalier ) ☐ Oculoskeletal dysplasia 2 (OSD2/DRD2) Dry Eye Curly Coat Syndrom (Samoyed) ☐ (Cavalier King Charles Spaniel) ☐ Osteogenesis imperfecta (OI) Duchenne muscular dystrophy (DMD) (Dachshund) ☐ (Cavalier King Charles Spaniel) ☐ Phosphofructokinase deficiency (PFK) / Epidermolytic Hyperkeratosis (EHK) Glycogen Storage Disease VII (GSD VII) ☐ () ☐ (American Cocker Spaniel, English Cocker Spaniel, , Episodic falling* ☐ German Spaniel, Whippet) (Cavalier King Charles Spaniel) Primary ciliary dyskinesia (PCD) Factor VII deficiency (Bobtail (Old English Sheepdog)) ☐ (, Alaskan Klee Kai, Beagle, Deerhound, Giant Schnauzer) ☐ Primary hyperoxaluria type I (PH1) ☐ Fucosidosis, alpha ☐ (Coton de Tulear ) (English Springer Spaniel ) Pug Dog Enzephalitis (PDE) / ☐ GM1-Gangliosidosis ☐ Necrotizing meningoencephalitis (NME) (Alaskan Husky) (Pug) Golden Retriever Muscular dystrophy (GRMD) ☐ Pyruvate dehydrogenase deficiency (PDPD) (Golden Retriever) (Clumber-Spaniel, Sussex-Spaniel) ☐

MOL-SUBDOG-FO-K5.4-338V4 (effective from 01.01.2020) Owner/Sender: ______Dog’s name: ______

Pyruvate kinase deficiency Von Willebrand disease II (vWDII) (Basenji, Cairn Terrier, West Highland White Terrier) ☐ (German Pointing Dog (short-haired), German Pointing Dog (wire-haired)) ☐ Severe combined immunodeficiency disease (SCID) Von Willebrand disease III (vWDIII) autosomal ☐ (, Shetland Sheepdog) ☐ (Jack Russell Terrier) *partner laboratory Severe combined immunodeficiency disease (XSCID) ☐ X-linked (Basset Hound, Welsh Corgi (Cardigan)) Trapped Neutrophil Syndrome (TNS) (Border Collie) ☐ Von Willebrand disease I (vWDI)

(Australian Labradoodle, Basset Hound, Bernese Mountain Dog, Coton de ☐

Tulear, Dachshund, Dobermann, Drentsche Partridge Dog, German

Shepherd Dog, Goldendoodle, Kerry Blue Terrier, Kromfohrländer, , Miniature Schnauzer, Papillon, Poodle, Poodle (Miniature), Poodle (Multicolor), Poodle (Toy), Rottweiler, Stabijhoun, Welsh Corgi (Pembroke), Wolfsspitz (Keeshond))

Eye Disorders Achromatopsia (cone degeneration (CD), hemeralopia) Multifocal retinopathy 2 (CMR2) (German Pointing Dog (short-haired)) ☐ (Coton de Tulear) ☐ Autosomal dominant progressive retinal atrophy (ADPRA) Primary hereditary cataract, early onset (HK/HSF4) (, Mastiff) ☐ (Australian Shepherd, Miniature American Shepherd, Miniature Australian ☐ Collie eye anomaly (CEA)* Shepherd) (Australian Shepherd, Border Collie, Boykin Spaniel, Collie (rough), Collie ☐ Primary hereditary cataract, juvenile (JHK/HSF4) (smooth), Lancashire heeler, Longhaired Whippet, Shetland Sheepdog, (Boston Terrier, French Bulldog, Staffordshire ) ☐ Silken Windhound) Progressive retinal atrophy (PRA1) Cone rod dystrophy 1 (crd1) (Papillon, Phalene) ☐ (American Staffordshire Terrier) ☐ Progressive retinal atrophy, CNGA1-related ☐ Cone rod dystrophy 2 (crd2) ☐ (Shetland Sheepdog) (American Pitt Bull Terrier) Progressive retinal atrophy, type 1 (XLPRA1) Cone rod dystrophy 3 (crd3)** X-linked ☐ (Irish Glen of Imaal Terrier) ☐ (Samoyed, Siberian Huskie, Weimaraner) Cone rod dystrophy 4 (crd4 / cord1) Progressive retinal atrophy, type 2 (XLPRA2) ☐ (Bolonka Zwetna, Dachshund, English Springer Spaniel) ☐ X-linked (Mongrel) Crd - Cone rod dystrophy ☐ Progressive retinal atrophy, type 3 (PRA3) (Dachshund (wire-haired)) ☐ Generalized progressive retinal atrophy (gPRA)*** (Tibetan Spaniel, ) ☐ Progressive rod-cone degeneration (prcd-PRA) package 1 ☐ Generalized progressive retinal atrophy (gPRA), DNA-profile, certificate, (American Cocker Spaniel, Australian Cattle Dog, Australian Shepherd, blood storage. Australian Stumpy Tail Cattle Dog, Bolonka Zwetna, Chesapeake Bay Generalized progressive retinal atrophy (gPRA)*** Retriever, Chihuahua, Chinese Crested Dog, English Cocker Spaniel, ☐ Entlebuch Cattle Dog, , Golden Retriever, Karelian Bear Schapendoes package 2 Dog, Kuvasz, Labrador Retriever, , Norwegian Elkhound Generalized progressive retinal atrophy (gPRA), certificate, blood storage. (black), Norwegian Elkhound (grey), Nova Scotia Duck Tolling Retriever, Golden Retriever PRA 1 (GR-PRA1) Poodle (Miniature), Poodle (Toy), Portugese Water Dog, Spanish Water Dog, (Golden Retriever) ☐ , ) Golden Retriever PRA 2 (GR-PRA2) Rod-cone dysplasia 1 (rcd1-PRA) ☐ (Golden Retriever) ☐ (Irish Red Setter, Irish Red and White Setter) Leber congenital amaurosis / Rod-cone dysplasia 1a (rcd1a-PRA) Congenital stationary night blindness (CSNB) ☐ (Sloughi) ☐ () Rod-cone dysplasia 3 (rcd3-PRA) Lens luxation (PLL) (Welsh Corgi (Cardigan)) ☐ (American Eskimo Dog, American Hairless Terrier, Australian Cattle Dog, ☐ Rod-cone dysplasia 4 (rcd4-PRA) Chinese Crested Dog, Chinese Foo Dog, , Fox Terrier (Toy), (, Irish Red Setter, Poodle, Tibetan Terrier) ☐ German Hunting Dog, Italiano Volpino, Jack Russell Terrier, , *partner laboratory Lancashire Heeler, Lucas Terrier, , Norfolk Terrier, **With the owner’s signature it is agreed that the crd3-results of all Glen of Imaal Norwich Terrier, , , Pug, Rat Terrier, Terrier may be given to , Teddy Roosevelt Terrier, Tenterfield Terrier, Tibetan ***With the owner’s signature it is agreed that the gPRA-results of all Schapendoes Terrier, , Westfalen Terrier, Yorkshire Terrier) may be given to the International Schapendoes Federation Multifocal retinopathy 1 (CMR1) (American Bulldog, Australian Shepherd, Boerboel, Bulldog, Bullmastiff, ☐ Dogo Canario, Dogue de Bordeaux, French Bulldog, Italian Cane Corso, Mastiff, Pyrenean Mountain Dog)

MOL-SUBDOG-FO-K5.4-338V4 (effective from 01.01.2020) Owner/Sender: ______Dog’s name: ______Coat Colors A-Locus: ay, aw, at, a-alleles H-Locus: Sable-fawn, wild type, wolf sable, black-and-tan, saddle-tan, ☐ Coat color harlequin ☐ recessive black (Great Dane) (all breeds) K-Locus: KB, ky-alleles, kbr is not tested B-Locus: Dominant black ☐ Brown, chocolate ☐ (all breeds) (all breeds) M-Locus: m, M, Mc, Ma, Mh-alleles D-Locus: Coat color merle ☐ Dilution ☐ (all breeds) (all breeds) E-Locus: EM-allele Melanistic mask ☐ (all breeds) E-Locus: Yellow, lemon, red, cream, apricot ☐ (all breeds)

Coat Type Curly coat Hair length (Airedale Terrier, American Water Spaniel, Bichon Frise, Border Collie, ☐ FGF5: c.284G>T; p.Cys95Phe mutation ☐ Boykin Spaniel, Chesapeake Bay Retriever, Chihuahua, Dachshund, (all breeds) Havanese, Kerry Blue Terrier, Kuvasz, Leonberger, Maltese, Pharaoh Hound, Portuguese Water Dog, Soft Coated Wheaten Terrier, Welsh Terrier, ) Furnishings / Improper coat (Bolonka Zwetna, Brussels Griffon, Chinese Crested, Dachshund, German ☐ Wirehaired , Havana Silk Dog, Havanese, Hungarian Wirehaired Vizsla, Jack Russell Terrier, Portuguese Water Dog, Soft-Coated Wheaten Terrier)

Test-packages Our test-packages allow cost-effective selection of multiple tests for genetic disorders, coat colors and coat types within one package. Per animal you can select up to 10 tests and each additional test ordered for the same animal increases discount.

The advantage our test package has, is, that only meaningful tests are performed for a particular animal. You as the customer select the tests needed for your specific animal. This avoids, in comparison to test-packages with fixed tests, screenings for mutations that are not validated for the breed or are meaningless and could have unfavorable effects on breeding decisions.

Caution! Combi-tests, DNA-profiles and tests performed by our partner laboratories cannot be selected within a test-package. If you order two and more genetic tests in addition to a Combi-test for the same animal, selecting the parameters of the Combi-test as single tests will result in a higher discount.

DNA-profile (ISAG) DNA-profile ☐ Certificate DNA-profile ☐  Each certificate (DNA-profile) will cost 7.14 € (incl. VAT).  Certificates can only be issued if Chip-no. are provided.  It is recommended that sampling is carried out by an independent authority (e.g. veterinarian or breed warden) to confirm sample origin.  Certificates will contain the marker-information (ISAG –standard) and can be used for identification and parentage verification.  The DNA-profile is stored for at least 10 years.

Parentage verification - please use the parentage verification submission form –

MOL-SUBDOG-FO-K5.4-338V4 (effective from 01.01.2020)