Catalog # Aliquot Size

U203-911-05 3 x 5 nmol U203-911-20 3 x 20 nmol U203-911-50 3 x 50 nmol

UBA3 (UBE1C) siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # U203-911

Lot # Z2109-12

Specificity Formulation UBA3 (UBE1C) siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human UBA3 (UBE1C) expression. shipped at room temperature.

Product Description Reconstitution Protocol UBA3 (UBE1C) siRNA is a pool of three individual synthetic Briefly centrifuge the tubes (maximum RCF 4,000g) to siRNA duplexes designed to knock-down human UBA3 collect lyophilized siRNA at the bottom of the tube. (UBE1C) mRNA expression. Each siRNA is 19-25 bases in Resuspend the siRNA in 50 µl of DEPC-treated water length. The accession number is BC022853. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot UBE1C, UBA3, hUBA3 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or UBA1 (UBE1) U201-30G below -20oC. After resuspension, siRNA stock solutions ≥2 UBA2 (SAE2) Protein U202-30G µM can undergo up to 50 freeze-thaw cycles without UBA3 (UBE1C) Protein U203-30G significant degradation. For long-term storage, it is UBA7 (UBE1L) Protein U207-30G recommended that the siRNA is stored at -70oC. For most Protein U06-54H favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background UBA3 (also known as UBE1C) or ubiquitin-like modifier UBA3 (UBE1C) siRNA Set I activating enzyme 3 is a member of the E1 ubiquitin- siRNA duplexes targeted against three exon regions activating enzyme family. UBA3 associates with AppBp1, an amyloid beta precursor protein binding protein, to Catalog # U203-911 form a heterodimer, and then the enzyme complex Lot # Z2109-12 activates NEDD8, a ubiquitin-like protein, which regulates Packaging Specifications 2.5 nmol/tube for 3 x 5nmol cell division, signaling and embryogenesis. The Format Lyophilized powder mechanism of NEDD8 activation by APPBP1-UBA3 shows Stability 1yr at -70oC from date of shipment a high degree of conservation with ubiquitin activation Storage & Shipping The lyophilized powder is stable for at by UBA1 (1). The NEDD8-activating enzyme, or NAE, least 4 weeks at room temperature. It is composed of NAE1 and UBA3 subunits, is an essential recommended that the lyophilized and resuspended siRNAs are stored at component for the treatment of cancer disease (2). or below -20oC. After resuspension, siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles References without significant degradation. For 1. Bohnsack, R. N. et.al: Conservation in the mechanism long-term storage, it is recommended of Nedd8 activation by the human AppBp1-Uba3 that the siRNA is stored at -70oC. For most favorable performance, avoid heterodimer. J. Biol. Chem. 278: 26823-26830, 2003. repeated handling and multiple 2. Soucy, T. A. et.al: An inhibitor of NEDD8-activating freeze/thaw cycles. enzyme as a new approach to treat cancer. Nature 458: 732-736, 2009.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.