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Laboratory Directors and Laboratory Staff From To: Laboratory Directors and Laboratory Staff From: Robert Rej, Ph.D. Date: March 6, 2012 Subject: Results of the February 6, 2012 Hematology Proficiency Test Enclosed are results from the hematology proficiency testing survey shipped February 6, 2012. Five samples were distributed for each test category: Routine Blood Counts (B56, B57, B58, B59, B60) Routine Coagulation (C56, C57, C58, C59, C60 - APTT, PT/INR and Fibrinogen assays) Cell Identification (356, 357, 358, 359, 360) Evaluation of Proficiency Test Results: Note: This report includes evaluation of the International Normalized Ratio (INR). Outlined below is a description of the process used to evaluate your laboratory's proficiency test results. A summary of your laboratory's performance for the three most recent surveys is also included with this report. Target Value: When possible, targets utilized are derived from all-participant mean values calculated by a robust statistical technique. In some cases, however, it is recognized that reagent, and/or instrument specific targets may be required and "peer group" specific targets are used where appropriate. An asterisk placed adjacent to the manufacturer name or instrument name indicates that a peer group was used in establishing targets and acceptable ranges. Not Gradable: Results for graded analytes for a few laboratories using unique instrument, reagent, or instrument/reagent combinations were considered "not gradable". For these laboratories pass credit (100%) has been issued. Since the laboratory is unable to participate in the NYS hematology proficiency test event as a graded participant, it is the responsibility of the laboratory to establish alternate means to verify the accuracy and precision of the test system for any ungraded analyte(s). Acceptable Range: Represents limits established using criteria specified by CLIA '88 regulations, allowing for rounding to appropriate significant digits. Results falling within this range are scored as 100%. Any result exceeding these limits is considered unsatisfactory and receives a score of 0%. Range Plots: The range plots graphically represent the relative distance of all results reported by your laboratory from the target value. Any result exceeding the high or low limit by >20% of the acceptable range is indicated by an asterisk (*). Analyte Score: Scores for both individual samples and overall analyte performance are provided. Laboratories must achieve an overall analyte score >80% in order to meet performance criteria for that analyte. Statistical Summary: Also enclosed is a statistical summary of participant data for the survey specimens. Mean and standard deviation (1 SD) values shown on the attached sheets are calculated by a robust statistical technique that does not assume a Gaussian distribution. Please note that standard deviation values are not used to determine acceptable ranges; CLIA '88 regulations established percentage limits for cellular and coagulation analytes. Cellular Hematology (CBC): Results for individual instruments, where the number of laboratories using those systems is three or greater, are provided. Coagulation: Results for individual instrument and reagent systems as well as instrument/reagent combinations, where the number of laboratories using those systems is three or greater, are provided. The use of brand and/or trade names in this report does not constitute an endorsement of the products on the part of the Wadsworth Center or the New York State Department of Health. So that this analysis can be as complete as possible, please review all future testings carefully and properly identify reagent and instrument systems used. If you have any questions regarding these reports or wish to obtain an additional copy, please contact the Hematology Laboratory at (518) 474-9878. You may also contact us by E-mail: [email protected] World Wide Web: Results from this proficiency test event and selected previous proficiency test events are available on the Hematology and Clinical Chemistry web page at: http://www.wadsworth.org/chemheme New York State Department of Health – Wadsworth Center Hematology Proficiency Test Event – February 6, 2012 Summary of Participant Responses Mean ± One Standard Deviation White Cell Count (x 109/L) Specimen: B56 Specimen: B57 Specimen: B58 Specimen: B59 Specimen: B60 Number [Code] Instrument -------------- -------------- -------------- -------------- -------------- ------- ------------------------------------- 4.84 ± 0.19 16.24 ± 0.78 9.10 ± 0.33 24.09 ± 1.08 4.04 ± 0.22 n = 411 [---] All Methods & Instruments <Instruments> 5.02 ± 0.21 16.36 ± 0.30 9.06 ± 0.18 24.39 ± 0.65 4.18 ± 0.20 n = 4 [ABF] Abbott Cell Dyn 3500 4.82 ± 0.24 16.00 ± 0.55 9.02 ± 0.51 23.17 ± 1.06 4.18 ± 0.15 n = 3 [ABG] Abbott Cell Dyn 1700 5.02 ± 0.04 17.38 ± 0.38 9.51 ± 0.23 25.41 ± 0.35 4.27 ± 0.09 n = 4 [ABJ] Abbott Cell Dyn 1800 5.03 ± 0.05 16.34 ± 0.51 9.24 ± 0.23 24.54 ± 0.56 4.05 ± 0.13 n = 7 [ABK] Abbott Cell Dyn 3200 4.94 ± 0.17 16.52 ± 0.55 9.18 ± 0.14 24.61 ± 0.36 4.08 ± 0.13 n = 12 [ABM] Abbott Cell Dyn 3700 4.91 ± 0.15 16.62 ± 0.40 9.29 ± 0.28 24.38 ± 0.62 4.12 ± 0.11 n = 15 [ABS] Abbott Cell Dyn Sapphire 5.00 ± 0.18 16.40 ± 0.47 9.23 ± 0.24 24.55 ± 0.68 4.12 ± 0.14 n = 17 [ABT] Abbott Cell Dyn Ruby 4.64 ± 0.10 15.97 ± 0.14 8.63 ± 0.05 22.61 ± 0.44 4.07 ± 0.05 n = 3 [ABU] Abbott Cell Dyn Emerald 4.56 ± 0.18 15.86 ± 0.79 8.73 ± 0.39 22.78 ± 0.82 3.74 ± 0.18 n = 21 [BTD] Siemens (Bayer)Advia 120 4.60 ± 0.21 16.15 ± 0.74 8.73 ± 0.35 23.08 ± 0.87 3.69 ± 0.21 n = 27 [BTE] Siemens (Bayer)Advia 2120 4.87 ± 0.08 16.10 ± 0.28 9.06 ± 0.09 24.27 ± 0.49 4.06 ± 0.07 n = 14 [CUL] Coulter UniCel DxH 800 4.72 ± 0.13 16.31 ± 0.49 8.93 ± 0.24 23.99 ± 0.50 3.85 ± 0.12 n = 8 [CUS] Coulter ACT 5 diff 4.87 ± 0.16 16.47 ± 0.35 9.15 ± 0.23 24.50 ± 0.61 4.23 ± 0.15 n = 28 [CUT] Coulter ACT series,not ACT5 diff 4.97 ± 0.14 17.41 ± 0.54 9.28 ± 0.35 25.48 ± 0.58 4.23 ± 0.12 n = 13 [CUW] Coulter HMX 4.81 ± 0.11 15.73 ± 0.46 9.12 ± 0.18 23.66 ± 0.77 4.12 ± 0.10 n = 72 [CUX] Coulter LH750,755 4.77 ± 0.10 15.46 ± 0.34 9.15 ± 0.14 23.39 ± 0.60 4.13 ± 0.09 n = 21 [CUY] Coulter LH 780 5.02 ± 0.09 17.29 ± 0.36 9.25 ± 0.19 25.57 ± 0.41 4.28 ± 0.11 n = 23 [CUZ] Coulter LH500 4.77 ± 0.17 16.60 ± 0.63 9.08 ± 0.39 24.75 ± 0.94 3.85 ± 0.17 n = 8 [ROB] ABX Pentra series 4.60 ± 0.09 15.51 ± 0.20 8.66 ± 0.10 22.31 ± 0.20 3.80 ± 0.09 n = 3 [SYB] Sysmex KX-21N 4.73 ± 0.14 15.63 ± 0.53 8.76 ± 0.28 23.38 ± 0.65 3.83 ± 0.14 n = 23 [SYO] Sysmex XE2100 4.89 ± 0.10 15.36 ± 0.22 8.73 ± 0.10 23.18 ± 0.37 3.83 ± 0.13 n = 7 [SYQ] Sysmex XE 2100D(Blood Center Only) 4.83 ± 0.19 15.95 ± 0.70 8.92 ± 0.43 23.86 ± 0.92 3.86 ± 0.19 n = 21 [SYA] Sysmex XE 5000 4.89 ± 0.13 16.46 ± 0.52 9.18 ± 0.25 24.42 ± 0.51 3.88 ± 0.12 n = 24 [SYI] Sysmex XT-1800i,XT-2000i 4.83 ± 0.16 16.64 ± 0.30 9.19 ± 0.18 24.60 ± 0.52 3.86 ± 0.11 n = 5 [SYV] Sysmex XT 4000i 5.08 ± 0.10 17.28 ± 0.36 9.52 ± 0.18 25.24 ± 0.64 4.07 ± 0.12 n = 16 [SYP] Sysmex XS-1000i,XS-1000iAL New York State Department of Health – Wadsworth Center Hematology Proficiency Test Event – February 6, 2012 Summary of Participant Responses Mean ± One Standard Deviation Red Cell Count (x 1012/L) Specimen: B56 Specimen: B57 Specimen: B58 Specimen: B59 Specimen: B60 Number [Code] Instrument -------------- -------------- -------------- -------------- -------------- ------- ------------------------------------- 3.034 ± 0.089 4.947 ± 0.111 4.517 ± 0.110 4.268 ± 0.100 4.010 ± 0.106 n = 410 [---] All Methods & Instruments <Instruments> 3.122 ± 0.055 5.066 ± 0.119 4.629 ± 0.038 4.378 ± 0.043 4.125 ± 0.072 n = 4 [ABF] Abbott Cell Dyn 3500 3.010 ± 0.072 5.033 ± 0.050 4.534 ± 0.103 4.312 ± 0.123 4.056 ± 0.088 n = 3 [ABG] Abbott Cell Dyn 1700 3.157 ± 0.068 5.018 ± 0.084 4.541 ± 0.072 4.391 ± 0.079 4.050 ± 0.058 n = 4 [ABJ] Abbott Cell Dyn 1800 3.192 ± 0.041 5.064 ± 0.056 4.688 ± 0.034 4.375 ± 0.030 4.175 ± 0.072 n = 7 [ABK] Abbott Cell Dyn 3200 3.099 ± 0.054 5.012 ± 0.088 4.599 ± 0.079 4.296 ± 0.079 4.068 ± 0.080 n = 12 [ABM] Abbott Cell Dyn 3700 3.111 ± 0.038 5.106 ± 0.102 4.643 ± 0.070 4.374 ± 0.090 4.094 ± 0.070 n = 15 [ABS] Abbott Cell Dyn Sapphire 3.070 ± 0.073 5.067 ± 0.114 4.603 ± 0.099 4.288 ± 0.090 4.039 ± 0.074 n = 17 [ABT] Abbott Cell Dyn Ruby 2.973 ± 0.023 4.944 ± 0.182 4.447 ± 0.185 4.249 ± 0.163 4.013 ± 0.123 n = 3 [ABU] Abbott Cell Dyn Emerald 3.077 ± 0.067 4.979 ± 0.104 4.583 ± 0.086 4.302 ± 0.093 4.034 ± 0.089 n = 21 [BTD] Siemens (Bayer)Advia 120 3.099 ± 0.053 5.026 ± 0.071 4.584 ± 0.066 4.353 ± 0.061 4.064 ± 0.072 n = 27 [BTE] Siemens (Bayer)Advia 2120 2.977 ± 0.051 4.809 ± 0.069 4.401 ± 0.041 4.180 ± 0.055 3.960 ± 0.056 n = 14 [CUL] Coulter UniCel DxH 800 3.073 ± 0.040 5.056 ± 0.052 4.590 ± 0.050 4.347 ± 0.048 4.047 ± 0.056 n = 8 [CUS] Coulter ACT 5 diff 2.925 ± 0.068 4.871 ± 0.097 4.414 ± 0.083 4.167 ± 0.082 3.914 ± 0.064 n = 27 [CUT] Coulter ACT series,not ACT5 diff 3.016 ± 0.077 4.929 ± 0.095 4.425 ± 0.082 4.209 ± 0.073 3.988 ± 0.114 n = 13 [CUW] Coulter HMX 2.953 ± 0.034 4.853 ± 0.048 4.428 ± 0.041 4.192 ± 0.041 3.907 ± 0.036 n = 72 [CUX] Coulter LH750,755 2.948 ± 0.029 4.851 ± 0.046 4.427 ± 0.046 4.195 ± 0.049 3.916 ± 0.043 n = 21 [CUY] Coulter LH 780 3.033 ± 0.064 4.914 ± 0.066 4.437 ± 0.058 4.210 ± 0.050 3.985 ± 0.078 n = 23 [CUZ] Coulter LH500 3.039 ± 0.068 5.065 ± 0.091 4.568 ± 0.088 4.379 ± 0.105 4.079 ± 0.057 n = 8 [ROB] ABX Pentra series 3.053 ± 0.023 4.943 ± 0.051 4.513 ± 0.034 4.220 ± 0.009 4.055 ± 0.036 n = 3 [SYB] Sysmex KX-21N 3.116 ± 0.036 4.966 ± 0.060 4.601 ± 0.045 4.365 ± 0.033 4.131 ± 0.044 n = 23 [SYO] Sysmex XE2100
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