microorganisms Article Population Structure of Non-ST6 Listeria monocytogenes Isolated in the Red Meat and Poultry Value Chain in South Africa Itumeleng Matle 1,2 , Thendo Mafuna 3,4 , Evelyn Madoroba 5 , Khanyisile R. Mbatha 2 , Kudakwashe Magwedere 6 and Rian Pierneef 4,* 1 Bacteriology Division, Agricultural Research Council-Onderstepoort Veterinary Research, Onderstepoort 0110, South Africa;
[email protected] 2 Department of Agriculture and Animal Health, Science Campus, University of South Africa, Florida 1709, South Africa;
[email protected] 3 Centre for Bioinformatics and Computational Biology, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0028, South Africa;
[email protected] 4 Biotechnology Platform, Agricultural Research Council-Onderstepoort Veterinary Research, Private Bag X 05, Onderstepoort 0110, Pretoria, South Africa 5 Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand, KwaDlangezwa 3886, South Africa;
[email protected] 6 Directorate of Veterinary Public Health, Department of Agriculture, Land Reform and Rural Development, Pretoria 0001, South Africa;
[email protected] * Correspondence:
[email protected]; Tel.: +27-12-5299-356 Received: 4 June 2020; Accepted: 28 June 2020; Published: 30 July 2020 Abstract: Meat products have been implicated in many listeriosis outbreaks globally, however there is a dearth of information on the diversity of L. monocytogenes isolates circulating in food products in South Africa. The aim of this study was to investigate the population structure of L. monocytogenes isolated in the meat value chain within the South African market. Based on whole-genome sequence analysis, a total of 217 isolates were classified into two main lineage groupings namely lineages I(n = 97; 44.7%) and II (n = 120; 55.3%).