Superlensing Microscope Objective Lens
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Introduction to CODE V: Optics
Introduction to CODE V Training: Day 1 “Optics 101” Digital Camera Design Study User Interface and Customization 3280 East Foothill Boulevard Pasadena, California 91107 USA (626) 795-9101 Fax (626) 795-0184 e-mail: [email protected] World Wide Web: http://www.opticalres.com Copyright © 2009 Optical Research Associates Section 1 Optics 101 (on a Budget) Introduction to CODE V Optics 101 • 1-1 Copyright © 2009 Optical Research Associates Goals and “Not Goals” •Goals: – Brief overview of basic imaging concepts – Introduce some lingo of lens designers – Provide resources for quick reference or further study •Not Goals: – Derivation of equations – Explain all there is to know about optical design – Explain how CODE V works Introduction to CODE V Training, “Optics 101,” Slide 1-3 Sign Conventions • Distances: positive to right t >0 t < 0 • Curvatures: positive if center of curvature lies to right of vertex VC C V c = 1/r > 0 c = 1/r < 0 • Angles: positive measured counterclockwise θ > 0 θ < 0 • Heights: positive above the axis Introduction to CODE V Training, “Optics 101,” Slide 1-4 Introduction to CODE V Optics 101 • 1-2 Copyright © 2009 Optical Research Associates Light from Physics 102 • Light travels in straight lines (homogeneous media) • Snell’s Law: n sin θ = n’ sin θ’ • Paraxial approximation: –Small angles:sin θ~ tan θ ~ θ; and cos θ ~ 1 – Optical surfaces represented by tangent plane at vertex • Ignore sag in computing ray height • Thickness is always center thickness – Power of a spherical refracting surface: 1/f = φ = (n’-n)*c -
Determination of Focal Length of a Converging Lens and Mirror
Physics 41- Lab 5 Determination of Focal Length of A Converging Lens and Mirror Objective: Apply the thin-lens equation and the mirror equation to determine the focal length of a converging (biconvex) lens and mirror. Apparatus: Biconvex glass lens, spherical concave mirror, meter ruler, optical bench, lens holder, self-illuminated object (generally a vertical arrow), screen. Background In class you have studied the physics of thin lenses and spherical mirrors. In today's lab, we will analyze several physical configurations using both biconvex lenses and concave mirrors. The components of the experiment, that is, the optics device (lens or mirror), object and image screen, will be placed on a meter stick and may be repositioned easily. The meter stick is used to determine the position of each component. For our object, we will make use of a light source with some distinguishing marking, such as an arrow or visible filament. Light from the object passes through the lens and the resulting image is focused onto a white screen. One characteristic feature of all thin lenses and concave mirrors is the focal length, f, and is defined as the image distance of an object that is positioned infinitely far way. The focal lengths of a biconvex lens and a concave mirror are shown in Figures 1 and 2, respectively. Notice the incoming light rays from the object are parallel, indicating the object is very far away. The point, C, in Figure 2 marks the center of curvature of the mirror. The distance from C to any point on the mirror is known as the radius of curvature, R. -
Far-Field Optical Imaging of Viruses Using Surface Plasmon Polariton
Magnifying superlens in the visible frequency range. I.I. Smolyaninov, Y.J.Hung , and C.C. Davis Department of Electrical and Computer Engineering, University of Maryland, College Park, MD 20742, USA Optical microscopy is an invaluable tool for studies of materials and biological entities. With the current progress in nanotechnology and microbiology imaging tools with ever increasing spatial resolution are required. However, the spatial resolution of the conventional microscopy is limited by the diffraction of light waves to a value of the order of 200 nm. Thus, viruses, proteins, DNA molecules and many other samples are impossible to visualize using a regular microscope. The new ways to overcome this limitation may be based on the concept of superlens introduced by J. Pendry [1]. This concept relies on the use of materials which have negative refractive index in the visible frequency range. Even though superlens imaging has been demonstrated in recent experiments [2], this technique is still limited by the fact that magnification of the planar superlens is equal to 1. In this communication we introduce a new design of the magnifying superlens and demonstrate it in the experiment. Our design has some common features with the recently proposed “optical hyperlens” [3], “metamaterial crystal lens” [4], and the plasmon-assisted microscopy technique [5]. The internal structure of the magnifying superlens is shown in Fig.1(a). It consists of the concentric rings of polymethyl methacrylate (PMMA) deposited on the gold film surface. Due to periodicity of the structure in the radial direction surface plasmon polaritons (SPP) [5] are excited on the lens surface when the lens is illuminated from the bottom with an external laser. -
How Does the Light Adjustable Lens Work? What Should I Expect in The
How does the Light Adjustable Lens work? The unique feature of the Light Adjustable Lens is that the shape and focusing characteristics can be changed after implantation in the eye using an office-based UV light source called a Light Delivery Device or LDD. The Light Adjustable Lens itself has special particles (called macromers), which are distributed throughout the lens. When ultraviolet (UV) light from the LDD is directed to a specific area of the lens, the particles in the path of the light connect with other particles (forming polymers). The remaining unconnected particles then move to the exposed area. This movement causes a highly predictable change in the curvature of the lens. The new shape of the lens will match the prescription you selected during your eye exam. What should I expect in the period after cataract surgery? Please follow all instructions provided to you by your eye doctor and staff, including wearing of the UV-blocking glasses that will be provided to you. As with any cataract surgery, your vision may not be perfect after surgery. While your eye doctor selected the lens he or she anticipated would give you the best possible vision, it was only an estimate. Fortunately, you have selected the Light Adjustable Lens! In the next weeks, you and your eye doctor will work together to optimize your vision. Please make sure to pay close attention to your vision and be prepared to discuss preferences with your eye doctor. Why do I have to wear UV-blocking glasses? The UV-blocking glasses you are provided with protect the Light Adjustable Lens from UV light sources other than the LDD that your doctor will use to optimize your vision. -
Holographic Optics for Thin and Lightweight Virtual Reality
Holographic Optics for Thin and Lightweight Virtual Reality ANDREW MAIMONE, Facebook Reality Labs JUNREN WANG, Facebook Reality Labs Fig. 1. Left: Photo of full color holographic display in benchtop form factor. Center: Prototype VR display in sunglasses-like form factor with display thickness of 8.9 mm. Driving electronics and light sources are external. Right: Photo of content displayed on prototype in center image. Car scenes by komba/Shutterstock. We present a class of display designs combining holographic optics, direc- small text near the limit of human visual acuity. This use case also tional backlighting, laser illumination, and polarization-based optical folding brings VR out of the home and in to work and public spaces where to achieve thin, lightweight, and high performance near-eye displays for socially acceptable sunglasses and eyeglasses form factors prevail. virtual reality. Several design alternatives are proposed, compared, and ex- VR has made good progress in the past few years, and entirely perimentally validated as prototypes. Using only thin, flat films as optical self-contained head-worn systems are now commercially available. components, we demonstrate VR displays with thicknesses of less than 9 However, current headsets still have box-like form factors and pro- mm, fields of view of over 90◦ horizontally, and form factors approach- ing sunglasses. In a benchtop form factor, we also demonstrate a full color vide only a fraction of the resolution of the human eye. Emerging display using wavelength-multiplexed holographic lenses that uses laser optical design techniques, such as polarization-based optical folding, illumination to provide a large gamut and highly saturated color. -
Depth of Focus (DOF)
Erect Image Depth of Focus (DOF) unit: mm Also known as ‘depth of field’, this is the distance (measured in the An image in which the orientations of left, right, top, bottom and direction of the optical axis) between the two planes which define the moving directions are the same as those of a workpiece on the limits of acceptable image sharpness when the microscope is focused workstage. PG on an object. As the numerical aperture (NA) increases, the depth of 46 focus becomes shallower, as shown by the expression below: λ DOF = λ = 0.55µm is often used as the reference wavelength 2·(NA)2 Field number (FN), real field of view, and monitor display magnification unit: mm Example: For an M Plan Apo 100X lens (NA = 0.7) The depth of focus of this objective is The observation range of the sample surface is determined by the diameter of the eyepiece’s field stop. The value of this diameter in 0.55µm = 0.6µm 2 x 0.72 millimeters is called the field number (FN). In contrast, the real field of view is the range on the workpiece surface when actually magnified and observed with the objective lens. Bright-field Illumination and Dark-field Illumination The real field of view can be calculated with the following formula: In brightfield illumination a full cone of light is focused by the objective on the specimen surface. This is the normal mode of viewing with an (1) The range of the workpiece that can be observed with the optical microscope. With darkfield illumination, the inner area of the microscope (diameter) light cone is blocked so that the surface is only illuminated by light FN of eyepiece Real field of view = from an oblique angle. -
Optical Negative-Index Metamaterials
REVIEW ARTICLE Optical negative-index metamaterials Artifi cially engineered metamaterials are now demonstrating unprecedented electromagnetic properties that cannot be obtained with naturally occurring materials. In particular, they provide a route to creating materials that possess a negative refractive index and offer exciting new prospects for manipulating light. This review describes the recent progress made in creating nanostructured metamaterials with a negative index at optical wavelengths, and discusses some of the devices that could result from these new materials. VLADIMIR M. SHALAEV designed and placed at desired locations to achieve new functionality. One of the most exciting opportunities for metamaterials is the School of Electrical and Computer Engineering and Birck Nanotechnology development of negative-index materials (NIMs). Th ese NIMs bring Center, Purdue University, West Lafayette, Indiana 47907, USA. the concept of refractive index into a new domain of exploration and e-mail: [email protected] thus promise to create entirely new prospects for manipulating light, with revolutionary impacts on present-day optical technologies. Light is the ultimate means of sending information to and from Th e arrival of NIMs provides a rather unique opportunity for the interior structure of materials — it packages data in a signal researchers to reconsider and possibly even revise the interpretation of zero mass and unmatched speed. However, light is, in a sense, of very basic laws. Th e notion of a negative refractive index is one ‘one-handed’ when interacting with atoms of conventional such case. Th is is because the index of refraction enters into the basic materials. Th is is because from the two fi eld components of light formulae for optics. -
Davis Vision's Contact Lens Benefits FAQ's
Davis Vision’s Contact Lens Benefits FAQ’s for Council of Independent Colleges in Virginia Benefits Consortium, Inc. How your contact lens benefit works when you visit a Davis Vision network provider. As a Davis Vision member, you are entitled to receive contact lenses in lieu of eyeglasses during your benefit period. When you visit a Davis Vision network provider, you will first receive a comprehensive eye exam (which requires a $15 copayment) to determine the health of your eyes and the vision correction needed. If you choose to use your eyewear benefit for contacts, your eye care provider or their staff will need to further evaluate your vision care needs to prescribe the best lens options. Below is a summary of how your contact lens benefit works in the Davis Vision plan. What is the Davis Vision Contact you will have a $60 allowance after a $15 copay plus Lens Collection? 15% discount off of the balance over that amount. You will also receive a $130 allowance toward the cost of As with eyeglass frames, Davis Vision offers a special your contact lenses, plus 15% discount/1 off of the balance Collection of contact lenses to members, which over that amount. You will pay the balance remaining after greatly minimizes out-of-pocket costs. The Collection your allowance and discounts have been applied. is available only at independent network providers that also carry the Davis Vision Collection frames. Members may also choose to utilize their $130 allowance You can confirm which providers carry Davis Vision’s towards Non-Collection contacts through a provider’s own Collection by logging into the member website at supply. -
Two-Photon Excitation Fluorescence Microscopy
P1: FhN/ftt P2: FhN July 10, 2000 11:18 Annual Reviews AR106-15 Annu. Rev. Biomed. Eng. 2000. 02:399–429 Copyright c 2000 by Annual Reviews. All rights reserved TWO-PHOTON EXCITATION FLUORESCENCE MICROSCOPY PeterT.C.So1,ChenY.Dong1, Barry R. Masters2, and Keith M. Berland3 1Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; e-mail: [email protected] 2Department of Ophthalmology, University of Bern, Bern, Switzerland 3Department of Physics, Emory University, Atlanta, Georgia 30322 Key Words multiphoton, fluorescence spectroscopy, single molecule, functional imaging, tissue imaging ■ Abstract Two-photon fluorescence microscopy is one of the most important re- cent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast im- ages and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine. CONTENTS INTRODUCTION ................................................ 400 HISTORICAL REVIEW OF TWO-PHOTON MICROSCOPY TECHNOLOGY ...401 BASIC PRINCIPLES OF TWO-PHOTON MICROSCOPY ..................402 Physical Basis for Two-Photon Excitation ............................ -
Confocal Microscopy
Confocal microscopy Chapter in Handbook of Comprehensive Biophysics ( in press 2011) Elsevier Brad Amos MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH UK e-mail [email protected] Gail McConnell University of Strathclyde , Centre for Biophotonics 161 Cathedral Street , Glasgow G4 0RE UK [email protected] Tony Wilson Dept. of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, UK. eMail: [email protected] 1 Introduction A confocal microscope is one in which the illumination is confined to a small volume in the specimen, the detection is confined to the same volume and the image is built up by scanning this volume over the specimen, either by moving the beam of light over the specimen or by displacing the specimen relative to a stationary beam. The chief advantage of this type of microscope is that it gives a greatly enhanced discrimination of depth relative to conventional microscopes. Commercial systems appeared in the 1980s and, despite their high cost, the world market for them is probably between 500 and 1000 instruments per annum, mainly because of their use in biomedical research in conjunction with fluorescent labelling methods. There are many books and review articles on this subject ( e.g. Pawley ( 2006) , Matsumoto( 2002), Wilson (1990) ). The purpose of this chapter is to provide an introduction to optical and engineering aspects that may be o f interest to biomedical users of confocal microscopy. Flying-spot Microscopes A confocal microscope is a special type of ‘flying spot’ microscope. Flying spot systems were developed in the 1950s by combining conventional microscopes with electronics from TV and military equipment. -
Lecture 37: Lenses and Mirrors
Lecture 37: Lenses and mirrors • Spherical lenses: converging, diverging • Plane mirrors • Spherical mirrors: concave, convex The animated ray diagrams were created by Dr. Alan Pringle. Terms and sign conventions for lenses and mirrors • object distance s, positive • image distance s’ , • positive if image is on side of outgoing light, i.e. same side of mirror, opposite side of lens: real image • s’ negative if image is on same side of lens/behind mirror: virtual image • focal length f positive for concave mirror and converging lens negative for convex mirror and diverging lens • object height h, positive • image height h’ positive if the image is upright negative if image is inverted • magnification m= h’/h , positive if upright, negative if inverted Lens equation 1 1 1 푠′ ℎ′ + = 푚 = − = magnification 푠 푠′ 푓 푠 ℎ 푓푠 푠′ = 푠 − 푓 Converging and diverging lenses f f F F Rays refract towards optical axis Rays refract away from optical axis thicker in the thinner in the center center • there are focal points on both sides of each lens • focal length f on both sides is the same Ray diagram for converging lens Ray 1 is parallel to the axis and refracts through F. Ray 2 passes through F’ before refracting parallel to the axis. Ray 3 passes straight through the center of the lens. F I O F’ object between f and 2f: image is real, inverted, enlarged object outside of 2f: image is real, inverted, reduced object inside of f: image is virtual, upright, enlarged Ray diagram for diverging lens Ray 1 is parallel to the axis and refracts as if from F. -
Imaging with Second-Harmonic Generation Nanoparticles
1 Imaging with Second-Harmonic Generation Nanoparticles Thesis by Chia-Lung Hsieh In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy California Institute of Technology Pasadena, California 2011 (Defended March 16, 2011) ii © 2011 Chia-Lung Hsieh All Rights Reserved iii Publications contained within this thesis: 1. C. L. Hsieh, R. Grange, Y. Pu, and D. Psaltis, "Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging," Opt. Express 17, 2880–2891 (2009). 2. C. L. Hsieh, R. Grange, Y. Pu, and D. Psaltis, "Bioconjugation of barium titanate nanocrystals with immunoglobulin G antibody for second harmonic radiation imaging probes," Biomaterials 31, 2272–2277 (2010). 3. C. L. Hsieh, Y. Pu, R. Grange, and D. Psaltis, "Second harmonic generation from nanocrystals under linearly and circularly polarized excitations," Opt. Express 18, 11917–11932 (2010). 4. C. L. Hsieh, Y. Pu, R. Grange, and D. Psaltis, "Digital phase conjugation of second harmonic radiation emitted by nanoparticles in turbid media," Opt. Express 18, 12283–12290 (2010). 5. C. L. Hsieh, Y. Pu, R. Grange, G. Laporte, and D. Psaltis, "Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle," Opt. Express 18, 20723–20731 (2010). iv Acknowledgements During my five-year Ph.D. studies, I have thought a lot about science and life, but I have never thought of the moment of writing the acknowledgements of my thesis. At this moment, after finishing writing six chapters of my thesis, I realize the acknowledgment is probably one of the most difficult parts for me to complete.