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Lipopolysaccharide (LPS) Core Biosynthesis in Proteus Mirabilis

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Lipopolysaccharide (LPS) Core Biosynthesis in Proteus Mirabilis Lipopolysaccharide (LPS) core biosynthesis in Proteus mirabilis Estudio de la biosíntesis del núcleo de lipopolisacarido (LPS) en Proteus mirabilis Eleonora Aquilini ADVERTIMENT. La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX (www.tdx.cat) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposició des d’un lloc aliè al servei TDX. No s’autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona autora. ADVERTENCIA. La consulta de esta tesis queda condicionada a la aceptación de las siguientes condiciones de uso: La difusión de esta tesis por medio del servicio TDR (www.tdx.cat) ha sido autorizada por los titulares de los derechos de propiedad intelectual únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro ni su difusión y puesta a disposición desde un sitio ajeno al servicio TDR. No se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al resumen de presentación de la tesis como a sus contenidos. En la utilización o cita de partes de la tesis es obligado indicar el nombre de la persona autora. WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tdx.cat) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reproduction with lucrative aims is not authorized neither its spreading and availability from a site foreign to the TDX service. Introducing its content in a window or frame foreign to the TDX service is not authorized (framing). This rights affect to the presentation summary of the thesis as well as to its contents. In the using or citation of parts of the thesis it’s obliged to indicate the name of the author. Departamento de Microbiología y Parasitología Clínica Facultad de Farmacia Universidad de Barcelona Lipopolysaccharide (LPS) core biosynthesis in Proteus mirabilis Estudio de la biosíntesis del núcleo de lipopolisacarido (LPS) en Proteus mirabilis Memoria presentada por Eleonora Aquilini para optar al grado de Doctora por la Universidad de Barcelona Programa de Doctorado Microbiología Ambiental y Biotecnología VºBº del director La doctoranda Dr. Miguel Regué Queralt Eleonora Aquilini Barcelona, Septiembre 2012 Acknowledgments Por encima de todo, me gustaría expresar el más sincero y profundo agradecimiento a mi director de tesis, Dr. Miguel Regué Queralt. Quiero darle las gracias por creer en mí desde el principio, cuando tener una pipeta en la mano no me resultaba tan natural y las estructuras químicas me parecían incomprensible jeroglíficos. Gracias por haberme dado no sólo la inmensa oportunidad, sino también los espacios y las herramientas para crecer como investigadora y científica. Quiero ademas agradecerle por haberme guiado paso a paso sin oprimir, por la constante y inconmensurable ayuda, por inspirarme con su pasión sin igual para la investigación, por ser paciente y con visión de futuro, y por último, por compartir conmigo su inmenso conocimiento en todos los momentos de investigación y de escritura de esta tesis. Un agradecimiento muy sentido a todos y cada uno de los estudiantes, doctorandos y profesores que participan a la vida diaria del laboratorio de Microbiología de la Facultad de Farmacia, es un placer por la mañana entrar por la puerta del Departamento y andar por nuestro largo pasillo. Gracias por hacer que sea un lugar agradable donde investigar. Algunas personas son irremplazables en un laboratorio, y es gracias a ellas que es un lugar ordenado, limpio y acogedor donde llegar. Gracias a Lucía para cuidar todos los aspectos burocráticos con la amabilidad, la precisión y la eficiencia que sólo ella sabe poner en ello. Gracias a M. Jesús y Carmen por el apoyo técnico inestimable, por la paciencia infinita en hacer más fácil y más rápido el trabajo de los demás, y sin duda... por saber siempre dónde está todo! Quiero agradecer el Dr. Juan Tomas por haberme dado la oportunidad de colaborar con su grupo de investigación, por aportar a este trabajo visión critica y intuición científica, por toda la ayuda a este proyecto y por la confianza en mi trabajo. Un sincero agradecimiento a la Dra. Susana Merino por su gran disponibilidad y los tremendamente valiosos consejos experimentales. Gracias en fin a todo el laboratorio 7 del Departamento de Microbiología de la Facultad de Biología: en particular gracias a Silvia y Natalia por ayudarme a dar los primeros pasos en el mágico mundo de la biología molecular y a Maite por el precioso soporte técnico. I would like to express my heartfelt gratitude to Professor Herry Mobley and to all members of the Mobley Research Group at University of Michigan. I am grateful for the chance to visit your lab and proud to have been part of your group. Thank you for your friendly welcome and for helping me to develop and realize part of the ideas of this thesis. Thanks to, and with all of you I experienced a different way of working, I joyfully faced another reality. GO Mobsters!!! This Michigan experience would not be so great without the amazing support of my Spanish Ann Arbor-based family: German, Maria, Manolo, Mario, Alfonso, Monica, Sole, Ramon, Sara, Marga y Alvaro thank you! Gracias por vuestro incondicional soporte desde siempre, por cuidarme sin casi conocerme, por no hacerme sentir nunca sola allá del otro lado del mundo, por abrirme las puertas del Km0, y finalmente gracias por acogerme a la vuelta! An enthusiast thanks to Christoph, for showing me the “other” wonderful Ann Arbor. Estoy en deuda con mis colegas de muchos años por proporcionar un entorno alegre, armonioso y estimulante en el que aprender y experimentar: Sara, Ornella, Carla, Guillermo, Nacho, Noelia, David, Andreu, Silvia, Xavi, Raquel, Markus y Eriel... el laboratorio no sería lo mismo sin vosotros: gracias! Quiero profundamente agradecer las personas que han hecho Barcelona entera un lugar muy especial para vivir (y para volver!) en todos esos años. Iria, mi compañera de piso favorita, cuanto hemos compartido! Gracias por tanto “conectar” conmigo desde un punto de vista siempre distinto, por hacerme ver las cosas de forma diferente, y definitivamente gracias por llevarme y introducirme en aquel maravilloso mundo que vive alrededor del lago de Banyoles, es por ti que hablo algo de Catalán! Vivi, gracias por haber vivido conmigo tantas locuras, las fiestas en Mirallers, las pausas en el trabajo, por tomar siempre todo con alegría y positividad, por saber revolucionar tu vida y la de los demás en cuestión de momentos y por acompañarme en aquel inolvidable viaje a Brasil. Alina, gracias por escuchar, por soltarme aquellas frases que me despiertan, por tener siempre un consejo y por estar dulcemente a mi lado día tras día. Carolina, gracias por ser mi amiga y compartir mas de aquella portuguesa amistad que nos ha conectado, por tener detalles siempre en el momento perfecto, por acogerme en el (ex) palacio de Collblanc cuando lo he necesitado. Montse, eres un sol, gracias por haberme llevado a tu mundo, por tener siempre atención, amistad y cuidado hacia mi. Cesar, gracias por los primeros tiempos, hay cosas que no se olvidan. Un enorme y sentido agradecimiento a Javi y Nacho por el cariño y para haberme acogido en sus casas, para darme un hogar donde volver al acabar el día. Todos vosotros habéis dejado huella, gracias por compartir conmigo incontables y inolvidables momentos, interminables charlas, incontenibles risas, y tantas experiencias! Es por todos vosotros que me siento en casa lejos de casa. Acknowledgments Un agradecimiento particular a Joana, por empezar siendo soporte y compañera de trabajo hasta volverse amiga de siempre y para siempre. Gracias por el apoyo sin fin, por haber hecho mis días mas alegres y con infinitas pistas sonoras, por estar allí mientras lloraba, mientras me reía y mientras lloraba de la risa, por aparecer en incontables fotos a mi lado, por tantos cumpleaños, celebraciones, conciertos, festivales, eventos y viajes juntas, por compartir conmigo tanto. Il mio piú intenso grazie all'amicizia piú importante, grazie a Valentina, per esserci sempre, con costanza e perseveranza, per non mancarmi mai, per essere il mio braccio destro e il mio cervello sinistro. Grazie per coltivare un'Amicizia che va oltre il tempo e i kilometri. Immensamente grazie. Un caloroso ringraziamento va a coloro che sono da sempre braccia aperte a cui tornare dopo ogni viaggio: Matteo, Elena, Stefania, Mari e Davide, grazie per essere stati una costante lontana ma sempre presente, per aspettare sempre il mio ritorno e per farmi sentire come se non me ne fossi mai andata. Il ringraziamento piú importante va alla mia Famiglia, per essere la mia Casa: appoggio e supporto incondizionato, certezza nell'incertezza, punto di partenza per ogni inizio e rifugio all'occorrenza. Mamma e Papá, grazie per comprendere il mio caparbio spirito un po' ribelle e nomade, per accettarlo e per assecondare sempre le mie ambizioni. Grazie per essere quella spinta propulsiva che mi accompagna da sempre. Eugenio, la nostra “fratellesca” complicitá e sintonia é insuperabile, ed é quanto di piú rassicurante conosca, grazie per rendermi ovunque e sempre sorella orgogliosa! Non c'é Casa se non ci sei tu ad accogliermi.
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