Cyprinidae: Smiliogastrinae)

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Cyprinidae: Smiliogastrinae) Iran. J. Ichthyol. (December 2020), 7(4): 306-313 Received: May 13, 2020 © 2020 Iranian Society of Ichthyology Accepted: October 30, 2020 P-ISSN: 2383-1561; E-ISSN: 2383-0964 DOI: 10.22034/iji.v7i4.514 http://www.ijichthyol.org Research Article Microscopic structure of the olfactory mucosa of onespot barb, Puntius terio (Cyprinidae: Smiliogastrinae) Saroj Kumar GHOSH 1Department of Zoology, Bejoy Narayan Mahavidyalaya, Itachuna, Hooghly-712 147, West Bengal, India *Email: [email protected] Abstract: Light microscopy and scanning electron microscopy were used to portray the cellular constituents lining the olfactory epithelium of teri barb, Puntius terio (Hamilton, 1822) (Cyprinidae: Smiliogastrinae). The olfactory organ holded 16 pairs of lamellae emitted from central raphe, consummate an oviform rosette. The multilamellar olfactory rosette was distinctively placed on the ground of nasal cavity and differentiated by specialized sensory and non-sensory territory. Sensory epithelium covered apical part and linguiform process of lamella while rest part is disguised by non-sensory epithelium. The sensory epithelium had two specific forms: ciliated receptor cells and microvillous receptor cells categorized by staining magnitude, texture and surface morphology. In divergence, the nonsensory epithelium was made up of stratified epithelial cells with microridges, cluster of ciliated non- sensory cells and secretory mucous cells. Basal cells were buried in the mucosa, presumed to be the progenitor of sensory or other supporting cells. Functional implication of cellular components on the olfactory lining was argued with feeding habits and behaviour of fish interested. Keywords: Olfactory organ, Surface feature, Histoarchitecture, Chemical senses. Citation: Ghosh, S. 2020. Microscopic structure of the olfactory mucosa of onespot barb, Puntius terio (Cyprinidae: Smiliogastrinae). Iranian Journal of Ichthyology 7(4): 306-313. 306 Ghosh.- Microscopic structure of the olfactory mucosa of onespot barb Iran. J. Ichthyol. (December 2020), 7(4): 306-313 Fig.1. Scanning electron micrographs of the olfactory organ of P. terio. (A) Olfactory rosette within nasal cavity (NC) shows equal number of olfactory lamellae (OL) containing linguiform processes (arrows) on either side of raphe (R). (B) Outer margins of olfactory lamella (OL) bear linguiform processes (arrows) covered with sensory epithelium (SE) remain attach to nasal cavity (NC). (C) Sensory part of lamella displays tips of ciliated receptor cells (RC) and microvillous receptor cells (MV). Ghosh.- Microscopic structure of the olfactory mucosa of onespot barb Fig.2. Transverse section of olfactory lamella of P. terio stained with Delafield’s Haematoxylin-Eosin (HE) and Mallory’s Triple (MT). (A) Olfactory lamella (OL) emitted from raphe (R) contains olfactory epithelium (OEP) and central core (CC) showing the arrangement of various cell types. Arrows mark blood vessels in central core (CC). (B) Nonsensory epithelium (NSE) contains sustentacular cells (SC), ciliated nonsensory cells (broken arrows) and mucous cell (MC). Note the presence of blood vessels (BV), connective tissue (CT) and pigment cells (solid arrows) in central core (CC) which separated from epithelium by basement membrane (BM). (C) Higher magnification of sensory epithelium (SE) shows closely packed ciliated receptor cells (C) with globular protrusions (P) and microvillous cells (solid arrows). Note the presence of nuclei (N) of ciliated cells and basal cells (BC) close to central core (CC). (D) Nonsensory epithelium (NSE) typified with sustentacular cells (SC), ciliated supporting cells (CSC), basal cells (BC) and series of mucous cells (MC) with secreted mucus (arrow haeds). CC indicates central core. Iran. J. Ichthyol. (December 2020), 7(4): 306-313 Fig.3. Surface view of olfactory mucosa (A) Nonsensory epithelium embossed with stratified epithelial cells (SC) with microridges and thick mat of ciliated nonsensory cells (CSC). Arrows indicate mucin masses. (B) Part of raphe shows stratified epithelial cells (SC) and opening of mucous cells (solid arrows) with secreted mucin droplets (arrow heads). Ghosh.- Microscopic structure of the olfactory mucosa of onespot barb Iran. J. Ichthyol. (December 2020), 7(4): 306-313 Iran. J. Ichthyol. (December 2020), 7(4): 306-313 Received: May 13, 2020 © 2020 Iranian Society of Ichthyology Accepted: October 30, 2020 P-ISSN: 2383-1561; E-ISSN: 2383-0964 DOI: 10.22034/iji.v7i4.514 http://www.ijichthyol.org مقاله پژوهشی ساختار میکروسکوپی مخاط بویایی گونه (Puntius terio (Cyprinidae: Smiliogastrinae ساروج کومار قوش گروه جانورشناسی، بجوی ناریان ماهاویدیاﻻیا، ایتاچونا، هوقلی 712147، بنگال غربی، هندوستان. چکیده: از میکروسکوپ نوری و الکترونی برای مصورسازی اجزای سلولی پوشاننده اپیتلیوم بویایی گونه (Puntius terio (Hamilton, 1822 استفاده شد. اندام بویایی 16 جفت تیغه خارجی از سوراخ مرکزی و یک زائده تخممرغی شکلی کامل داشت. زائده تیغهای چند ﻻیه بویایی در بخش پایین حفره بینی قرار داشت و به واسطه جایگاههای گیرندههای حسی و غیرحسی از یکدیگر متفاوت بودند. اپیتلیوم حسی قسمت فوقانی و تیغهای را پوشانده در حالی که قسمت باقیمانده توسط اپیتلیوم غیرحسی پوشیده شده است. اپیتلیوم حسی دو شکل خاص داشت: سلولهای گیرنده مژهدار و سلولهای گیرنده میکروویلی که بر اساس مقدار رنگ، بافت و شکل سطحی طبقهبندی میشوند. در تغییرات، اپیتلیوم غیر حسی از سلولهای اپیتلیال طبقه طبقه ریزلبه، دسته سلولهای غیرحسی مژهدار و سلولهای مخاطی ترشحی ساخته شده است. سلولهای پایه توسط مخاط پوشانده شدند، فرض شد که این ماده از سلولهای حسی یا سایر سلولهای پشتیبانی کننده باشد. پیامدهای عملکردی اجزای سلولی در محفظه بویایی تحت تاثیر ترجیحات عادات غذایی و رفتار ماهی استدﻻل شد. کلماتکلیدی: اندام بویایی، خصوصیات سطحی، ساختار بافتی، حواس شیمیایی. .
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