ANTIBODIES FOR RESEARCH

Immune Checkpoint Blockade STING Pathway Investigate co-stimulatory and co-inhibitory Interrogate the STING Pathway by Western blot, IHC, molecules with high-quality, multi-application or Flow. STING (Stimulator of Interferon Genes) is a validated antibodies. Co-inhibitory and co-stimulatory detector of intracellular viral molecules and double molecules play a critical role in activation and stranded DNA. Activation of STING triggers tumor cell recognition and killing. Along with MHC/TCR phosphorylation of downstream NAK/TBK1 and IRF3 to activate immunity and a type I interferon response. The engagement, co-signaling molecules direct the outcome ability of STING agonists to activate a potent anti-tumor of T cell activation. In the context of cancer, tumor cells immune response has driven increased interest in the exploit the upregulation of co-inhibitory molecules to pathway for . promote their own survival and avoid immune recognition.

T cell APC dsDNA OX40 OX40L CD27 CD27L Costimulatory CD137 CD137L cGAS Type I IFNs CD28 CD80/CD86 +ATP +GTP TCR MHC cGAMP p65 p60 CTLA-4 CD80/CD86 NF-kB PD1 PD-L1/PD-L2 STING CoinhibitoryCD80 PD-L1 P P IRF3 TBK1 IRF3 IRF3 TIM3 GAL9

VISTA/PD-1H Antibody PD-L1 Antibody IRF3 Antibody (2G3) STING Antibody NBP1-88967 MAB1561 NBP1-47812 NBP2-24683

IHC: VISTA staining of IHC: PD-L1 staining IHC: IRF3 staining of IHC: STING staining of human kidney. of human colon cancer. adenocarcinoma of colon tissue. human breast tumor. (3 Publications) (1 Publication) (5 Publication)

LAG-3 Antibody PD-1 Antibody NAK/TBK1 Antibody RelA/NFkB p65 Antibody NBP1-97657 AF1086 NB100-56705 NB100-2176

Flow: LAG-3 FITC staining IHC: PD-1 staining of IHC: TBK1 on human IHC: RelA staining of human in resting and PHA activated human lymph node. testis. DLBCL showing nuclear expression . in the tumor cells. (9 Publications) (9 Publications) (17 Publications) (16 Publications) p: 303.730.1950 • f: 303.730.1966 • [email protected] • www.novusbio.com Purinergic Signaling Myeloid Cell and The Quantify ATP levels and Probe Purinergic Signaling. Similar to inhibitory members of the B7 Understand the tumor microenvironment and family, adenosine signaling dampens anti-tumor myeloid cell biology with Novus antibodies. immunity. The purine nucleotide, ATP, is converted by Suppressive myeloid cells in the tumor microenvironment extracellular receptors to adenosine. This molecule inhibit anti-tumor immunity. By secreting suppressive and signals through G-protein coupled receptors, including angiogenic molecules, tumor-associated Mouse the A2AHuman receptor, to mediate immunosuppresive + + and myeloid-derived suppressor cells promote tumor CD11b responses.Cd11b It has been demonstrated that adenosine Gr-Ly1/Ly-6G- HLA-Dr- growth and survival. Understanding myeloid cell biology is + receptor +blockade enhances anti-tumor immunity. Ly-6C CD14 key to developing improved . BecauseLin- of its potential to regulate immunity, adenosine CD66b- signaling- is considered next generation immune CD15 checkpoint blockade. Mouse Human CD11b+ Cd11b+ + - - Pannexin-1 MonocyticGranulocytic Gr-1/Ly-6G HLA Dr ATP - - Ly-6C CD14 Mouse monocytic: Mouse granulocytic: - + high lo Lin P2X CD11b+ Gr-1/Ly-6Glo Ly-6Chigh CD11b Gr-1/Ly-6G Ly-6C CD66b+ 2 Human monocytic: Human granulocytic: CD15+ CD39 P2Y - + + – Lin–CD11b+ CD14– CD15+ ADP Lin CD11b CD14 CD15 + + – CD73 AR CD33+ CD66b– HLA-DR– CD33 CD66b HLA-DR ATP

NT 2 3 AMP AMP Adenosine Adenosine Stimulatory vs. Suppressive Myeloid Cells

A1 receptor A2A receptor A2B receptor A3 receptor Stimulatory Suppressive IL-12hi IL-10lo IL-12lo IL-10hi Gsor Gq Gs G or G G or G i q i o ROS TGF-B RNI Arginase

Adenosine A2a Receptor CD39 Antibody CD11b Antibody HLA-DR Antibody NB300-597 NBP1-90071 NB110-40766 NB100-77855

ICC/IF: Adenosine A2a ICC/IF: CD39 antibody Flow: Detection of CD11 Flow: HLA-DR expression receptor antibody staining in staining in human aortic b/c in fixed Hela cells. in BDCM cells. the cytoplasm of U251 cells. valve endothelial cells. (14 Publications) (26 Publications)

CD73 Antibody HIF-1 alpha Antibody CD68 Antibody iNOS Antibody NBP1-85740 NB100-105 NB100-683 NB300-605

IHC: CD73 antibody staining WB; HIF-1 alpha analysis IHC staining of CD68 in WB: iNOS staining in of human duodenum. of COS-7 nuclear extracts. human spleen. stimulated astrocytes. (3 Publications) (580 Publications) (26 Publications) (12 Publications)

p: 303.730.1950 • f: 303.730.1966 • [email protected] • www.novusbio.com