Weis, Judith S., Ph.D
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Behavioral Analysis of Microphallus Turgidus Cercariae in Relation to Microhabitat of Two Host Grass Shrimp Species (Palaemonetes Spp.)
W&M ScholarWorks VIMS Articles 2017 Behavioral analysis of Microphallus turgidus cercariae in relation to microhabitat of two host grass shrimp species (Palaemonetes spp.) PA O'Leary Virginia Institute of Marine Science OJ Pung Follow this and additional works at: https://scholarworks.wm.edu/vimsarticles Part of the Aquaculture and Fisheries Commons Recommended Citation O'Leary, PA and Pung, OJ, "Behavioral analysis of Microphallus turgidus cercariae in relation to microhabitat of two host grass shrimp species (Palaemonetes spp.)" (2017). VIMS Articles. 774. https://scholarworks.wm.edu/vimsarticles/774 This Article is brought to you for free and open access by W&M ScholarWorks. It has been accepted for inclusion in VIMS Articles by an authorized administrator of W&M ScholarWorks. For more information, please contact [email protected]. Vol. 122: 237–245, 2017 DISEASES OF AQUATIC ORGANISMS Published January 24 doi: 10.3354/dao03075 Dis Aquat Org Behavioral analysis of Microphallus turgidus cercariae in relation to microhabitat of two host grass shrimp species (Palaemonetes spp.) Patricia A. O’Leary1,2,*, Oscar J. Pung1 1Department of Biology, Georgia Southern University, Statesboro, Georgia 30458, USA 2Present address: Department of Aquatic Health Sciences, Virginia Institute of Marine Science, PO Box 1346, State Route 1208, Gloucester Point, Virginia 23062, USA ABSTRACT: The behavior of Microphallus turgidus cercariae was examined and compared to microhabitat selection of the second intermediate hosts of the parasite, Palaemonetes spp. grass shrimp. Cercariae were tested for photokinetic and geotactic responses, and a behavioral etho- gram was established for cercariae in control and grass shrimp-conditioned brackish water. Photo - kinesis trials were performed using a half-covered Petri dish, and geotaxis trials used a graduated cylinder. -
Composition, Seasonality, and Life History of Decapod Shrimps in Great Bay, New Jersey
20192019 NORTHEASTERNNortheastern Naturalist NATURALIST 26(4):817–834Vol. 26, No. 4 G. Schreiber, P.C. López-Duarte, and K.W. Able Composition, Seasonality, and Life History of Decapod Shrimps in Great Bay, New Jersey Giselle Schreiber1, Paola C. López-Duarte2, and Kenneth W. Able1,* Abstract - Shrimp are critical to estuarine food webs because they are a resource to eco- nomically and ecologically important fish and crabs, but also consume primary production and prey on larval fish and small invertebrates. Yet, we know little of their natural history. This study determined shrimp community composition, seasonality, and life histories by sampling the water column and benthos with plankton nets and benthic traps, respectively, in Great Bay, a relatively unaltered estuary in southern New Jersey. We identified 6 native (Crangon septemspinosa, Palaemon vulgaris, P. pugio, P. intermedius, Hippolyte pleura- canthus, and Gilvossius setimanus) and 1 non-native (P. macrodactylus) shrimp species. These results suggest that the estuary is home to a relatively diverse group of shrimp species that differ in the spatial and temporal use of the estuary and the adjacent inner shelf. Introduction Estuarine ecosystems are typically dynamic, especially in temperate waters, and comprised of a diverse community of resident and transient species. These can include several abundant shrimp species which are vital to the system as prey (Able and Fahay 2010), predators during different life stages (Ashelby et al. 2013, Bass et al. 2001, Locke et al. 2005, Taylor 2005, Taylor and Danila 2005, Taylor and Peck 2004), processors of plant production (Welsh 1975), and com- mercially important bait (Townes 1938). -
Salinity Tolerances for the Major Biotic Components Within the Anclote River and Anchorage and Nearby Coastal Waters
Salinity Tolerances for the Major Biotic Components within the Anclote River and Anchorage and Nearby Coastal Waters October 2003 Prepared for: Tampa Bay Water 2535 Landmark Drive, Suite 211 Clearwater, Florida 33761 Prepared by: Janicki Environmental, Inc. 1155 Eden Isle Dr. N.E. St. Petersburg, Florida 33704 For Information Regarding this Document Please Contact Tampa Bay Water - 2535 Landmark Drive - Clearwater, Florida Anclote Salinity Tolerances October 2003 FOREWORD This report was completed under a subcontract to PB Water and funded by Tampa Bay Water. i Anclote Salinity Tolerances October 2003 ACKNOWLEDGEMENTS The comments and direction of Mike Coates, Tampa Bay Water, and Donna Hoke, PB Water, were vital to the completion of this effort. The authors would like to acknowledge the following persons who contributed to this work: Anthony J. Janicki, Raymond Pribble, and Heidi L. Crevison, Janicki Environmental, Inc. ii Anclote Salinity Tolerances October 2003 EXECUTIVE SUMMARY Seawater desalination plays a major role in Tampa Bay Water’s Master Water Plan. At this time, two seawater desalination plants are envisioned. One is currently in operation producing up to 25 MGD near Big Bend on Tampa Bay. A second plant is conceptualized near the mouth of the Anclote River in Pasco County, with a 9 to 25 MGD capacity, and is currently in the design phase. The Tampa Bay Water desalination plant at Big Bend on Tampa Bay utilizes a reverse osmosis process to remove salt from seawater, yielding drinking water. That same process is under consideration for the facilities Tampa Bay Water has under design near the Anclote River. -
Development of a Denaturing High-Performance Liquid Chromatography (DHPLC) Assay to Detect Parasite Infection in Grass Shrimp Palaemonetes Pugio
Original Article Fish Aquat Sci 15(2), 107-115, 2012 Development of a Denaturing High-Performance Liquid Chromatography (DHPLC) Assay to Detect Parasite Infection in Grass Shrimp Palaemonetes pugio Sang-Man Cho* Department of Aquaculture and Aquatic Science, Kunsan National University, Gunsan 573-701, Korea Abstract In developing a useful tool to detect parasitic dynamics in an estuarine ecosystem, a denaturing high-performance liquid chroma- tography (DHPLC) assay was optimized by cloning plasmid DNA from the grass shrimp Palaemonetes pugio, and its two para- sites, the trematode Microphallus turgidus and bopyrid isopod Probopyrus pandalicola. The optimal separation condition was an oven temperature of 57.9°C and 62-68% of buffer B gradient at a flow rate of 0.45 mL/min. A peptide nucleic acid blocking probe was designed to clamp the amplification of the host gene, which increased the amplification efficiency of genes with low copy numbers. Using the DHPLC assay with wild-type genomic, the assay could detect GC Gram positive bacteria and the bopyrid iso- pod (P. pandalicola). Therefore, the DHPLC assay is an effective tool for surveying parasitic dynamics in an estuarine ecosystem. Key words: Liquid chromatography assay, Grass shrimp Palaemonetes pugio,Trematode Microphallus turgidus, Bopyrid iso- pod Probopyrus pandalicola Introduction Tremendous endeavors have been carried out to moni- in accelerating the breakdown of detritus, and also transferring tor coastal ecosystem pollution. Because the impact on hu- energy from producer to the top levels of the estuarine food mans and ecosystems is ambiguous, biomonitoring such as chain (Anderson, 1985). It also serves as a detritus decompos- the ‘Mussel Watch’ Program (Kim et al., 2008), is a power- er, primary and secondary consumer, as well as crucial dietary ful method to measure the dynamics of lethal chemicals in component for carnivore fish, birds, mammals, and larger the environment. -
Bear Country" Basket for Our Undefeated High Is the Scope and Sequence Other People
. m m t . * . < v et Pages 14,15 Minit-Ed » . ^ *1.Stev.ens<>n "» ** *** » ■ * * w w ^ ash when he was photographed showing deflB,U!,y ■ * * * resoling. W e other day Gary Hart got some publicity ipUeagewlth a photograph that showed art « e of hfe shoes but two of them ia aeed of e a h e r tepairs Whether such publicity staging gets a candidate votes is a question. About the <my thing they prove is that the candidate and THE SOUTH-BERGEN REVIEW mdn t h y e his foot in his mouth — when the Photograph was taken, that is! 25C Second-Class postage paid at Ruthertotd. VOL. 62 NO. 33 Published at 251 Ridge Rd . Lyndhurst THURSDAY, MARCH 8, 1984 Subscription S8 DO Published Weekly Gagliardi Blasts Critics Eight In Hall Of F ame The Lyndhurst High for the legendary Coot the Bergen County meet High School Hall of Fame O f Meadowlands Plant School Athletic Hall of Manisera on Lyndhurst “s and was the runnerup in for his playing abilities, Dear Editor, monitor and supervise the homes on this property dated March 4, 1984 to the Fame has announced it 1949 undefeated football the Group 3 state meet. His Librera is a retired As one of the com operation. This demand will result in a loss (rf tax Bergen Record, in addition will induct eight players, team. Carini, a Lyndhurst track seasons included two educator missioners who voted to was agreed to by Dr. ratables when you con to his usual scathing coaches or contributors at resident, is an executive firsts in the mile in the ELIZABETH LINDSAY negotiate with the Bergen Sadat, director of the sider emergency services diatribe leveled against its fifth induction dinner on with Prudential Insurance Passaic Valley Conference — Class of 1931 — Miss County Utilities Authority N .J.D .E.P. -
Nematostella Vectensis Class: Anthozoa, Hexacorallia
Phylum: Cnidaria Nematostella vectensis Class: Anthozoa, Hexacorallia Order: Actiniaria, Nynantheae, Athenaria Starlet sea anemone Family: Edwardsiidae Taxonomy: Nematostella vectensis was 1975). There is a single ventral siphonoglyph described by Stephenson in 1935. (Williams 1975). Nematostella pellucida is a synonym (Hand Oral Disc: There is no inner 1957). In the larger taxonomic scale, the ring of tentacles, and there are no subclass Zoantharia has been synonymized siphonoglyphs, on the oral disc. with Hexacorallia (Hoeksema 2015). Tentacles: Tentacles are retractile, cylindrical, and tapered. They are Description not capitate, or knobbed. Though they can Medusa: No medusa stage in Anthozoans vary from 12-18, there are usually 16 Polyp: (Stephenson 1935; Fautin and Hand 2007). Size: The column (Fig. 1) can be up to There are 6-7 outer (exocoelic) tentacles that 15 mm long in the field, but can grow much are longer than inner (endocoelic) tentacles, longer (160 mm) when raised in the and are often reflexed down the column (they laboratory (Hand and Uhlinger 1992; Fautin can be longer than column). The inner and Hand 2007). The maximum diameter is 4 tentacles can be raised above the mouth (Fig. mm at the base near the bulb (physa) (Hand 1), and can have white spots on their inner 1957) and increases to 8 mm at the crown of edges (Crowell 1946). Nematosomes can be tentacles; the diameter is not often this large, seen moving inside the tentacles. and a more average diameter of the column is Mesenteries: Mesenteries are 2.5 mm. vertical partitions (eight in this species) below Color: The anemone is white and the gullet and visible through the column. -
Embryonic Development and Cardiac Morphology of the Grass Shrimp, Palaemonetes Pugio Holthuis, 1949 (Decapoda, Caridea, Palaemonidae): Embryonic Staging
Crustaceana 86 (1) 16-33 EMBRYONIC DEVELOPMENT AND CARDIAC MORPHOLOGY OF THE GRASS SHRIMP, PALAEMONETES PUGIO HOLTHUIS, 1949 (DECAPODA, CARIDEA, PALAEMONIDAE): EMBRYONIC STAGING BY A. L. ROMNEY1) and C. L. REIBER School of Life Sciences, University of Nevada, Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-4004, U.S.A. ABSTRACT Decapod crustaceans are known for their variation in developmental patterns and their success- ful adaptation to diverse environmental conditions. The grass shrimp, Palaemonetes pugio Holthuis, 1949, inhabits the brackish waters off the Atlantic and Gulf coasts of North America and is a crit- ically important component of the food chain in these ecosystems. A clear description and under- standing of early embryonic developmental processes is necessary in order for this species to be used for future physiological, ecological, developmental and toxicological investigations. Here, we establish a uniform staging scheme for the embryonic period of development in grass shrimp using an 8-stage sequence. Under constant conditions (20°C, 32 ppt sea water), mean clutch size was 190 (±5) embryos per female and mean time for embryonic development (fertilization to eclosion) was 13 days. Morphological changes and staging were documented using photomicroscopy (still and video). Also described are cardiac morphological changes associated with embryonic development. The morphometrics of both embryonic development and cardiac ontogeny are provided. The mor- phological features described for P. pugio throughout embryonic development and the standardized embryonic staging scheme can be used to compare developmental patterns and timing when inves- tigating questions related to an array of biological disciplines making this species a stronger model system. -
Hydrodynamic Regulation of Salt Marsh Contributions to Aquatic Food Webs
Vol. 490: 37–52, 2013 MARINE ECOLOGY PROGRESS SERIES Published September 17 doi: 10.3354/meps10442 Mar Ecol Prog Ser Hydrodynamic regulation of salt marsh contributions to aquatic food webs Ronald Baker1,2,*, Brian Fry3,5, Lawrence P. Rozas4, Thomas J. Minello1 1NOAA National Marine Fisheries Service, SEFSC, Galveston Laboratory, 4700 Avenue U, Galveston, Texas 77551, USA 2Centre for Tropical Water and Aquatic Ecosystem Research, School of Marine and Tropical Biology, James Cook University, and CSIRO Land and Water, ATSIP Building, James Cook University, Townsville, Queensland 4811, Australia 3Department of Oceanography and Coastal Sciences, LSU, Baton Rouge, Louisiana 70803, USA 4NOAA National Marine Fisheries Service, SEFSC, Estuarine Habitats and Coastal Fisheries Center, 646 Cajundome Boulevard, Lafayette, Louisiana 70506, USA 5Present address: Australian Rivers Institute, Griffith University, 170 Kessels Road, Brisbane, Queensland 4111, Australia ABSTRACT: Vegetated salt marsh habitats are widely considered critical for supporting many species of nekton, yet direct evidence of the processes controlling marsh habitat use for most species remains elusive. We related salt marsh flooding patterns and nekton trophic dynamics among 14 sites spanning 2500 km across the northern Gulf of Mexico (GoM) and southern Atlantic coasts of the USA. Functional access for nekton to marsh vegetation (edge flooded to ≥5 cm depth) ranged from <40% of the time at some central GoM sites to >90% access in the western GoM and Pamlico Sound. Food web mixing models based on stable isotope analysis show that the impor- tance of Spartina trophic support for common nekton may be regulated by the duration of marsh surface flooding. In particular, the potential contribution of Spartina production was positively related to indices of marsh surface flooding for brown shrimp Farfantepenaeus aztecus, white shrimp Litopenaeus setiferus, small (≤60 mm carapace width) blue crabs Callinectes sapidus, grass shrimp Palaemonetes pugio, and killifish Fundulus heteroclitus/grandis. -
Hudson-Raritan Estuary Comprehensive Restoration Plan
Hudson-Raritan Estuary Comprehensive Restoration Plan Version 1.0 Volume I June 2016 and In partnership with Contributing Organizations Government • Cary Institute of Ecosystem Studies • U.S. Army Corps of Engineers, New York District • City University of New York • The Port Authority of New York & New Jersey • Cornell University • National Park Service • Dowling College • National Oceanic and Atmospheric Administration • Harbor School • U.S. Department of Agriculture, Natural Resources • Hudson River Foundation Conservation Service • Hunter College • U.S. Environmental Protection Agency • Kean University • U.S. Fish & Wildlife Service • Liberty Science Center • Empire State Development Corporation • Manhattan College • New Jersey Department of Environmental • Montclair State University Protection, Division of Fish and Wildlife • New Jersey City University • New Jersey Department of Transportation • New Jersey Marine Science Consortium • New Jersey Meadowlands Commission • New York-New Jersey Harbor & Estuary Program • New York State Department of Environmental • Queens College Conservation • Rutgers University and Institute of Marine and • New York State Department of State, Division of Coastal Sciences Coastal Resources • State University of New York at Stony Brook • New York City Mayor’s Office • State University of New York – College of • New York City Department of Parks and Recreation Environmental Science and Forestry • New York City Department of Environmental • Stevens Institute of Technology Protection • St. John’s University -
Public Health Assessment: VENTRON/VELSICOL SITE
Public Health Assessment Final Release VENTRON/VELSICOL SITE WOOD-RIDGE/CARLSTADT, BERGEN COUNTY, NEW JERSEY EPA FACILITY ID: NJD980529879 Prepared by New Jersey Department of Health OCTOBER 4, 2016 Prepared under a Cooperative Agreement with the U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Toxic Substances and Disease Registry Division of Community Health Investigations Atlanta, Georgia 30333 THE ATSDR PUBLIC HEALTH ASSESSMENT: A NOTE OF EXPLANATION This Public Health Assessment was prepared by ATSDR’s Cooperative Agreement Partner pursuant to the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA or Superfund) section 104 (i)(6) (42 U.S.C. 9604 (i)(6)), and in accordance with our implementing regulations (42 C.F.R. Part 90). In preparing this document, ATSDR’s Cooperative Agreement Partner has collected relevant health data, environmental data, and community health concerns from the Environmental Protection Agency (EPA), state and local health and environmental agencies, the community, and potentially responsible parties, where appropriate. In addition, this document has previously been provided to EPA and the affected states in an initial release, as required by CERCLA section 104 (i)(6)(H) for their information and review. The revised document was released for a 30-day public comment period. Subsequent to the public comment period, ATSDR’s Cooperative Agreement Partner addressed all public comments and revised or appended the document as appropriate. The public health assessment has now been reissued. This concludes the public health assessment process for this site, unless additional information is obtained by ATSDR’s Cooperative Agreement Partner which, in the agency’s opinion, indicates a need to revise or append the conclusions previously issued. -
Population Genetics Analysis of the Grass Shrimp Palaemonetes Pugio Using Single Strand Conformation Polymorphism
Georgia Southern University Digital Commons@Georgia Southern Electronic Theses and Dissertations Graduate Studies, Jack N. Averitt College of Fall 2004 Population Genetics Analysis of the Grass Shrimp Palaemonetes Pugio Using Single Strand Conformation Polymorphism Melody A. Flowers Follow this and additional works at: https://digitalcommons.georgiasouthern.edu/etd Part of the Life Sciences Commons Recommended Citation Flowers, Melody A., "Population Genetics Analysis of the Grass Shrimp Palaemonetes Pugio Using Single Strand Conformation Polymorphism" (2004). Electronic Theses and Dissertations. 738. https://digitalcommons.georgiasouthern.edu/etd/738 This thesis (open access) is brought to you for free and open access by the Graduate Studies, Jack N. Averitt College of at Digital Commons@Georgia Southern. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of Digital Commons@Georgia Southern. For more information, please contact [email protected]. POPULATION GENETICS ANALYSIS OF THE GRASS SHRIMP PALAEMONETES PUGIO USING SINGLE STRAND CONFORMATION POLYMORPHISM by MELODY A. FLOWERS (Under the Direction of Oscar J. Pung and Quentin Fang) ABSTRACT Population genetics studies reveal significant information concerning an organism that lead to a better understanding of the microevolutionary forces acting upon the organism. Little is known about the genetic structure of grass shrimp, Palaemonetes pugio, populations or the effects the parasite Microphallus turgidus has on it. The objective of this study was to determine the amount of genetic diversity of the shrimp populations based on locality and parasite load. In order to examine the genetics of P. pugio, shrimp DNA was extracted and scanned using single strand conformation polymorphism. Results revealed P. -
Growth of Grass Shrimp, Palaemonetes Pugio, in a Contaminated and an Uncontaminated Site
Copyright Warning & Restrictions The copyright law of the United States (Title 17, United States Code) governs the making of photocopies or other reproductions of copyrighted material. Under certain conditions specified in the law, libraries and archives are authorized to furnish a photocopy or other reproduction. One of these specified conditions is that the photocopy or reproduction is not to be “used for any purpose other than private study, scholarship, or research.” If a, user makes a request for, or later uses, a photocopy or reproduction for purposes in excess of “fair use” that user may be liable for copyright infringement, This institution reserves the right to refuse to accept a copying order if, in its judgment, fulfillment of the order would involve violation of copyright law. Please Note: The author retains the copyright while the New Jersey Institute of Technology reserves the right to distribute this thesis or dissertation Printing note: If you do not wish to print this page, then select “Pages from: first page # to: last page #” on the print dialog screen The Van Houten library has removed some of the personal information and all signatures from the approval page and biographical sketches of theses and dissertations in order to protect the identity of NJIT graduates and faculty. ABSTRACT GROWTH OF GRASS SHRIMP, PALAEMONETES PUGIO, IN A CONTAMINATED AND AN UNCONTAMINATED SITE by Suruchi Bhan Previous experiments have found that grass shrimp, Palaemonetes pugio, from a contaminated site, Piles Creek (PC). in Linden. New Jersey, are larger than those from a relatively pristine reference site, Sheepshead Creek, located in Tuckerton (T), New Jersey.