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US5356803.Pdf ||||||||||||I|| US005356803A United States Patent (19) 11 Patent Number: 5,356,803 Carpenter et al. (45) Date of Patent: ck Oct. 18, 1994 (54). ANTIMICROBIAL COMPOSITION FOREIGN PATENT DOCUMENTS ESSESSASEAND 0179449 4/1986 European Pat. Off. 192401 8/1986 European Pat. Off. ANTMICROBAL AGENT 0197622 10/1986 European Pat. Off. 75 Inventors: Richard S. Carpenter, Cincinnati, 8:22 A3, E. Ea. g Ohio; Pushkaraj J. Lad, San Mateo uropean Pat. Off. VV, 2937964. 11/1982 Fed. Rep. of Germany. Calif.; Ann M. Wolff, Cincinnati, 2122301 of 0000 France . Ohio 24483.51 9/1980 France. 73 Assignees: Genencor International, Inc., So. San 4. 8609459 12/198733, Ene Francisco, Calif.; The Procter & 55-153709 11/1980 Japan. Gamble Company, Cincinnati, Ohio 57-075926 5/1982 Japan. * Notice: The portion of the term of this patent 59-088086215498 12/19855/1984 JE. subsequent to Aug. 24, 2010 has been 61-015827 1/1986 Japan. disclaimed. 62-044-180 2/1987 Japan. 62-248487 10/1987 Japan . 21 Appl. No.: 869,356 (List continued on next page.) 22) Filed: Mar. 30, 1992 l OTHER PUBLICATIONS Related U.S. Application Data Hughes, R. C., 1983 Glycoproteins, pp. 23-26 (Chap (63) doned.continuation of ser, No. 428,362, Oct. 27, 1989, aban- "",(List continuedNY) on next page.) 51) Int, Cl. ......................... C12N 9/24; C12N 9/40; C12N 9/78; D06M 16/00 Primary Examiner-David M. Naff 52 U.S. Cl. .................................... 435/200; 435/208; Assistant Examiner-Michael V. Meller 435/264; 435/206,435/227; 252/DIG. 12; Attorney, Agent, or Firm-Margaret A. Horn 252/17412 58) Field of Search ............... 435/264, 208, 200, 206, 57 ABSTRACT 435/227; 252/DIG. 12, 174.12 An antimicrobial composition consisting essentially of (56) References Cited from about 1 ppm to about 1200 ppm of a Type II endo glycosidase and from about 0.5 ppm to about 1200 ppm U.S. PATENT DOCUMENTS of an antimicrobial agent is disclosed. The preferred 4,062,941 12/1977 Davies ................................... 424/94 Type II endoglycosidases to be used in the invention are 4,144,327 3/1979 Davies et al. 424/94.61 Endo-Ds Endo-H, Endo-F and PNGaseF. The pre : 3. y - - - - - - - - - - - - - - : ferred antimicrobial agents are bactericides, fungicides 4,619,8259. 9. 10/1986 EigeneSO et al................................ 424/49 and algicides. The composition can be used in the form 4,639,375 l/1987 Tsai ....................................... 26/49 of personal care or household cleaning products such as 4,710,313 12/1987 Miyajima et al. ................... 252/105 liquid soap, hard surface cleaner, laundry detergent, 4,749,511 6/1988 Lad et al. ............ ... 252/174.12 anti-acne medication, deodorant, shampoo, face cream, 4,801,451 1/1989 Hellgren et al.................. 424/94.63 mouthwash, dentifrice and denture cleaner. 4,812,404 3/1989 Kuboki ................ ... 435/175 4,939,123 7/1990 Neeser et al. ........................... 514/8 5,041,236 8/1991 Carpenter et al. ............. 252/17412 15 Claims, 28 Drawing Sheets 5,356,803 Page 2 FOREIGN PATENT DOCUMENTS Glycoprotein of Friend Murine Leukemia Virus”, Eur. 63-002911 1/1988 Japan. J. Biochem., vol. 143, pp. 531-539. 1942236 3/1971 Netherlands . Hsieh et al., 1983, “Selective Cleavage by En 693380 6/1970 South Africa. do-g-N-acetylglucosaminidase H at Individual Glyco 86O7738 4/1987 South Africa . sylation Sites of Sindbis Virion Envelope Glycopro 1272135 4/1972 United Kingdom. teins', The Journal of Biological Chemistry, vol. 258, 1311375 3/1973 United Kingdom . No. 4, issue of Feb. 25, pp. 2555-2561. 2120240 11/1983 United Kingdom . Chipman et al., 1969, “Mechanism of Lysozyme Ac tion', Science, vol. 165, pp. 454-465. OTHER PUBLICATIONS Haskell et al., 1970, "Neuraminidase Inhibition and Thotakura et al., 1987, “Enzymatic Deglycosylation of Viral Chemotherapy”, Journal of Medicinal Chemistry, Glycoproteins', Methods in Enzymology, vol. 138, pp. vol. 13, No. 4, pp. 697-704. 350-359. Tute, M. S., 1970, "The Inhibition of Viral Neuramini Boehringer Mannheim, Biochemicals Division, Indian dase by 1-Phenoxymethyl-3,4-dihydroisoquinolines', apolis, Ind., "Glycohydrolases'. Part II, vol. 13, pp. 48-51. Cohen, 1986, "B-N-Acetylglucosaminidase from Chang et al., 1986, "Expression and Size Heterogeneity of a 63 Kilodalton Membrane Glycoprotein During Phycomyces Blakesleeanus', Plant Science, vol.43, pp. Growth and Transformation of Leishmania Mexicana 93-101. Amazonensis’, Molecular and Biochemical Parasitol Abeles et al., 1970, "Preparation and Purification of ogy, vol. 18, pp. 197-210. Glucanase and Chitinase from Bean Leaves', Plant Tarentino, A. L. and Maley, F., "Purification and Prop Physiology, vol. 47, pp. 129-134. erties of an Endo-g-N-acetyl glucosaminidase from Chaiet et al., 1970, "Isolation of a Pure Dextranase from Streptomyces griseus', The Journal of Biological Chem Penicillium funiculosun', Applied Microbiology, vol. istry vol. 249, No. 3, Issue of Feb. 10, pp. 811-817, 1974. 20, No. 3, pp. 421-426. Neuberger et al., 1967, "Inhibition of Lypozyme by Montague, M.D., 1964, “The Enzymic Degradation of N-Acyl-D-Glucosamine Derivatives', Nature, vol. Cell Walls of Streptococcus Faecalis', Biochim. Bio 215, Jul. 29 Issue, pp. 524-525. phys. Acta, vol. 86, pp. 588-595. Thotakura et al., Methods in Enzymology (138), pp. Anderson et al., 1964, “Studies on Carbohydrate-Meta 350-359, 1987. bolizing Enzymes', Biochem. J., vol. 90, pp. 30-35. Merck Index, 10th ed. (1983), Merck & Co., Inc., Rah Geyer et al., 1984, “Structure of the Oligosaccharides way, N.J. (pp. 135-136, 293, 1018-1020, 1315-1316, Sensitive to Endo-3-N-acetylglucosaminidase Hin the 1380-1381). U.S. Patent Oct. 18, 1994 Sheet 1 of 28 5,356,803 N-LINKED CORE STRUCTURE Asn-GlcNAc-glCno C-Man Z D-LINKED CORE STRUCTURE -GONAc-Neuac - GolNAc-Got Ser- DR, -GONAC-Go-Neu Act Thr NeuNAC -GolNAc-Got L-FuC Figure-1 U.S. Patent Oct. 18, 1994 Sheet 2 of 28 5,356,803 TYPE II ENDDGLYCSIDASE SUBSTRATE X Glycopeptidase-F Asn-GlcNAc-GlcNAc-Mon-vMan-w (PNGOSeF) A N CFuC) Mary Z Mor X M. N. Endo-H, F, D, CI Asn-GNAGlcNAc-Marvt A Mon-Y W N Z Endo-F-got type Asn-GlcNAC-Go-Mor Man A N Mon Ser Enco-O-N- Or golNAc-Gol Acetylgalactosorinidase Thr Endo-R-N- V Golactosidose R1-GlcNAc-Gol-GlcNAc-R2 Figure-2 U.S. Patent 5,356,803 ç-ºun61-I I U.S. Patent 5,356,803 3.LIS350k}/\VETTO #-eun61-I U.S. Patent 5,356,803 3.SVGIISTJOATEJDJOINE LNDO-EUIISDJOÅT19ONINI? LS8ITÈSEONº wg-eun612 gg-ean513 OG-ºun614 U.S. Patent Oct. 18, 1994 Sheet 7 of 28 5,356,803 FIGURE-6A. U.S. Patent Oct. 18, 1994 Sheet 8 of 28 5,356,803 FIGURE-6B U.S. Patent Oct. 18, 1994 Sheet 9 of 28 5,356,803 FIGURE-7A U.S. Patent Oct. 18, 1994 Sheet 10 of 28 5,356,803 FIGURE-7B U.S. Patent Oct. 18, 1994 Sheet 11 of 28 5,356,803 FIGURE-7C U.S. Patent Oct. 18, 1994 Sheet 12 of 28 5,356,803 FIGURE-7D U.S. Patent Oct. 18, 1994 Sheet 13 of 28 5,356,803 FIGURE-7E U.S. Patent Oct. 18, 1994 Sheet 14 of 28 5,356,803 FIGURE-7F U.S. Patent Oct. 18, 1994 Sheet 15 of 28 5,356,803 FIGURE -7G U.S. Patent Oct. 18, 1994 Sheet 16 of 28 5,356,803 FIGURE -7H U.S. Patent Oct. 18, 1994 Sheet 17 of 28 5,356,803 (INV0+IuiddH–opu? 0+uidd3NICIIXEHNDTHO 0+uidd3NICIIXBHNDTHO 09 g–Qun614 02 O 9 U.S. Patent Oct. 18, 1994 Sheet 18 of 28 5,356,803 OGudd3NICIIXEHNDTHO LINDJOH-OKOUETID]>| 002viddH–opu3CINº OGudd 3NICIIXEHNDTHO an5136–3 O O. U.S. Patent Oct. 18, 1994 Sheet 19 of 28 5,356,803 3.4 A LG CFU E.Coll VS, Enco-H 3.3 3.2 3.1 3.0 2.9 2,8 2.7 2,6 2.5 2,4 2,3 2.2 2.1. 2.0 1.9 18 1.7 16 15 14 1.3 O 40 80 120 160 200 240 280 Figure-10A(Endo-H) ppm 3 A LOG CFU E.coli VS, Endo-Hi 6 200, 500, 1000 ppm Endo-H O 200 400 600 800 1000 (Endo-H) ppm Figure-10B U.S. Patent Oct. 18, 1994 Sheet 20 of 28 5,356,803 U.S. Patent Oct. 18, 1994 Sheet 21 of 28 5,356,803 £1–HHn?IH U.S. Patent Oct. 18, 1994 Sheet 22 of 28 5,356,803 ANTMICROBIAL EFFECTS THROUGH THE WASH LDGS GROWTH 2L/D5 1/D5 1/05/A 2TM D5 D5 D5 = DETERGENT L = IRGASAN T = TRICLDCARBAN A = E 2 D R 1 =2A DR A RESPECTIVELY Figure-14 U.S. Patent Oct. 18, 1994 Sheet 23 of 28 5,356,803 FIGURE - 15A U.S. Patent Oct. 18, 1994 Sheet 24 of 28 5,356,803 U.S. Patent Oct. 18, 1994 Sheet 25 of 28 5,356,803 FIGURE-16A U.S. Patent Oct. 18, 1994 Sheet 26 of 28 5,356,803 FIGURE-6B U.S. Patent Oct. 18, 1994 Sheet 27 of 28 5,356,803 FIGURE-17B U.S. Patent Oct. 18, 1994 Sheet 28 of 28 5,356,803 FIGURE - 18B 5,356,803 1. 2 et al. (1977), Botanica Marina, 20, 13-17. As reported ANTMCROBAL COMPOSITION CONTAINING therein, Pseudomonas species isolated from sea water TYPE II ENDOGLYCOSIDASE AND was adhered to glass slides. Thereafter, the slides were ANTIMICROBAL AGENT treated with either pronase, trypsin, a-amylase (a Type I endoglycosidase), or lysozyme (also a Type I endo This is a continuation of application Ser.
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