DOCSLIB.ORG

SNP Technology This Page Intentionally Left Blank PLANT GENOTYPING II SNP Technology

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
SNP Technology This Page Intentionally Left Blank PLANT GENOTYPING II SNP Technology PLANT GENOTYPING II SNP Technology This page intentionally left blank PLANT GENOTYPING II SNP Technology Edited by Robert J. Henry Centre for Plant Conservation Genetics Southern Cross University Lismore, New South Wales, Australia CABI is a trading name of CAB International CABI Head Office CABI North American Office Nosworthy Way 875 Massachusetts Avenue Wallingford 7th Floor Oxfordshire OX10 8DE Cambridge, Massachusetts 02139 UK USA Tel: +44 (0)1491 832111 Tel: +1 617 395 4056 Fax: +44 (0)1491 833508 Fax: +1 617 354 6875 E-mail: [email protected] E-mail: [email protected] Web site: www.cabi.org ©CAB International 2008. All rights reserved. No part of this publication may be reproduced in any form or by any means, electronically, mechanically, by photo- copying, recording or otherwise, without the prior permission of the copyright owners. A catalogue record for this book is available from the British Library, London, UK. Library of Congress Cataloging-in-Publication Data Plant genotyping II : SNP technology / edited by Robert J. Henry. p. cm. Includes bibliographical references and index. ISBN 978-1-84593-382-1 (alk. paper) 1. Plant genome mapping. 2. Genetic polymorphisms. I. Henry, Robert J. QK981.45.P565 2008 581.3'5--dc22 2007041585 ISBN: 978 1 84593 382 1 Typeset by SPi, Pondicherry, India. Printed and bound in the UK by Biddles Ltd, King’s Lynn. Contents Contributors vii Preface ix 1 SNP Discovery in Plants 1 K.J. Edward, R.L. Poole and G.L. Barker 2 SNPs and Their Use in Maize 30 A. Rafalski and S. Tingey 3 Rare SNP Discovery with Endonucleases 44 M.J. Cross 4 Sequence Polymorphisms in the Flanking Regions of 68 Microsatellite Markers G. Ablett and R.J. Henry 5 SNP Discovery by Ecotilling Using Capillary 78 Electrophoresis F. Eliott, G. Cordeiro, P.C. Bundock and R.J. Henry 6 Genotyping by Allele-specifi c PCR 88 D.L.E. Waters, P.C. Bundock and R.J. Henry 7 The MassARRAY System for Plant Genomics 98 D. Irwin 8 Mutation Screening 114 L. Izquierdo v vi Contents 9 Nanotechnology: The Future of Cost-effective 133 Plant Genotyping J.A. Pattemore, M. Trau and R.J. Henry 10 Functionally Associated Molecular Genetic Markers 154 for Temperate Pasture Plant Improvement J.W. Forster, N.O.I. Cogan, M.P. Dobrowolski, M.G. Francki, G.C. Spangenberg and K.F. Smith 11 Genotyping for Rice Eating Qualities 187 L.M.T. Bradbury, D.L.E. Waters and R.J. Henry 12 Towards Universal Loci for Plant Genotyping 195 T. Pacey-Miller 13 DNA Banks as a Resource for SNP Genotyping 207 N. Rice, S. Kasem and R.J. Henry 14 DNA Extraction from Plant Tissue 219 S. Kasem, N. Rice and R.J. Henry 15 Future Prospects for Plant Genotyping 272 R.J. Henry Index 281 Contributors G. Ablett, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] G.L. Barker, University of Bristol, School of Biological Sciences, Woodland Road, Bristol BS8 1UG, UK. E-mail: Gary [email protected] L.M.E. Bradbury, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: l.bradbury.10@ scu.edu.au P.C. Bundock, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] N.O.I. Cogan, Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, La Trobe Research and Development Park, Bundoora, Victoria, 3083, Australia. E-mail: [email protected] G. Cordeiro, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] M.J. Cross, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] M.P. Dobrowolski, Department of Primary Industries, Biosciences Research Division, Hamilton Centre, Hamilton, Victoria 3300, Australia and Molecular Plant Breeding Cooperative Research Centre, Australia. E-mail: mark. [email protected] K.J. Edward, University of Bristol, School of Biological Sciences Woodland Road, Bristol BS8 1UG, UK. E-mail: [email protected] F. Eliott, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] J.W. Forster, Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, La Trobe Research and Development Park, Bundoora, Victoria, 3083, Australia. E-mail: [email protected] vii viii Contributors M.G. Francki, Department of Agriculture and Food Western Australia, 3 Baron- Hay Court, South Perth, Western Australia 6151 and State Agricultural Biotechnology Centre, Murdoch University, Murdoch, Western Australia 6983, Australia and Molecular Plant Breeding Cooperative Research Centre, Australia. E-mail: [email protected] R.J. Henry, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] D. Irwin, Sequenom Herston, Queensland, Australia. E-mail: [email protected] L. Izquierdo, Griffith University, Lismore, New South Wales, Australia. E-mail: [email protected] S. Kasem, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] T. Pacey-Miller, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: toni.pacey-miller@ scu.edu.au J.A. Pattemore, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] R.L. Poole, University of Bristol, School of Biological Sciences Woodland Road, Bristol BS8 1UG, UK. E-mail: [email protected] A. Rafalski, DuPont Crop Genetics Research, Experimental Station E353, Route 141 and Henry Clay Road, Wilmington, Delaware. E-mail: antoni.rafalski@cgr. dupont.com N. Rice, Australian Plant DNA Bank, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] K.F. Smith, Department of Primary Industries, Biosciences Research Division, Hamilton Centre, Hamilton, Victoria 3300, Australia and Molecular Plant Breeding Cooperative Research Centre, Australia. E-mail: kevin.f.smith@dpi. vic.gov.au G.C. Spangenberg, Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, La Trobe Research and Development Park, Bundoora, Victoria, 3083, Australia. E-mail: german.spangenberg@dpi. vic.gov.au S. Tingey, DuPont Crop Genetics Research, Experimental Station E353, Route 141 and Henry Clay Road, Wilmington, Delaware. E-mail: Scott.V.Tingey@USA. dupont.com M. Trau, Centre for Nanotechnology and Biomaterials University of Queensland, St Lucia, Queensland, Australia. E-mail: [email protected] D.L.E. Waters, Centre for Plant Conservation Genetics, Southern Cross University, Lismore, New South Wales, Australia. E-mail: [email protected] Preface Plant Genotyping: The DNA Fingerprinting of Plants was published in 2001. The book was based upon a workshop held at Southern Cross University in 2000 with additional contributions from some authors who did not attend the work- shop. The techniques available for plant DNA analysis have advanced consid- erably in the time since the original volume was published. Plant Genotyping II: SNP Technology aims to describe some of the important recent developments in this field. This book is based upon a second workshop held recently to review progress in this area. Recent developments focus on high- throughput methods and generally target single nucleotide polymorphism (SNP) discovery and analysis. R.J. Henry Centre for Plant Conservation Genetics Southern Cross University ix This page intentionally left blank 1 SNP Discovery in Plants K.J. EDWARD, R.L. POOLE AND G.L. BARKER Introduction Marker-assisted selection (MAS) using DNA-based molecular markers is now used by virtually all commercial plant and animal breeding companies (reviewed in Henry, 2001). Molecular markers can be used to identify both useful genotypes for inclusion in breeding programmes and interesting prog- eny for further study. However, the cost of MAS is a limitation when com- pared to phenotypically driven conventional breeding. In plants microsatellite markers are currently the most popular type of marker system; however, their development is resource-intensive and they are difficult to multiplex. Recently there has been considerable interest in the development of single nucleotide polymorphism (SNP pronounced SNiP)-based marker systems. More recently the use of SNPs has been fuelled by the explosion in the number of expressed sequence tags (ESTs) available in the various databases and this trend is set to continue for the foreseeable future (Varshney et al., 2004). SNPs are the most common form of sequence variation between indi- viduals within a species (reviewed in Brookes, 1999). New SNPs are continu- ally arising within every cell of an organism but most are removed through the action of the enzymatic process known as mismatch repair (MMR), and hence SNPs which become fixed within a germ line and a population are simply those mutations which have escaped the repair process (reviewed in Kunkel and Erie, 2005). As mutations are the source of all SNPs, the number of SNPs within a specific population reflects the forward and reverse muta- tion rates, the number of generations that separate the population from its closest relative and the number of independent progenitors of that popula- tion. The number and distribution of SNPs are also influenced by the past and present selection pressures applied to the population including any bot- tlenecks through which it has passed. Hence, while mutations probably occur at broadly similar frequencies in most species, the number of SNPs present ©CAB International 2008. Plant Genotyping II: SNP Technology (ed. R.J. Henry) 1 2 K.J. Edward et al. within a population will often show considerable variation, and it should come as no surprise to any plant breeder that different plant species will have different SNP frequencies and that these frequencies will vary within different regions of the genome.
Load more

Recommended publications
  • Supplemental Statement
    Received by NSD/FARA Registration Unit 06/21/2021 2:43:26 PM OMB No. 1124-0002; Expires July 31, 2023 U.S. Department of Justice Supplemental Statement Washington, dc 20530 Pursuant to the Foreign Agents Registration Act of 1938, as amended For 6 Month Period Ending May 31, 2021 (Insert dale) I - REGISTRANT 1. (a) Name of Registrant (b) Registration Number Reston Translator, LLC 6490 (c) Primary Business Address On file with DOJ 2. Has there been a change in the information previously furnished in connection with the following? (a) If an individual: (1) Residence address(es) Yes □ No □ (2) Citizenship Yes □ No □ (3) Occupation Yes □ No □ (b) If an organization: (1) Name Yes □ No 0 (2) Ownership or control Yes □ No 0 (3) Branch offices Yes □ No 0 (c) Explain fully all changes, if any, indicated in Items (a) and (b) above. IF THE REGISTRANT IS AN INDIVIDUAL, OMIT RESPONSES TO ITEMS 3, 4, 5, AND 6. 3. If the registrant previously filed an Exhibit C*1, state whether any changes therein have occurred during this 6 month reporting period. Yes □ No 0 If yes, has the registrant filed an updated Exhibit C? Yes □ No □ If no, please file the updated Exhibit C. 1 The Exhibit C, for which no printed form is provided, consists of a true copy of the charter, articles of incorporation, association, and by laws of a registrant that is an organization. (A waiver of the requirement to file an Exhibit C may be obtained for good cause upon written application to the Assistant Attorney General, National Security Division, U.S.
    [Show full text]
  • De.Sputniknews.Com
    de.sputniknews.com The German-language site of Sputnik News, a Russian state-owned news agency that publishes propaganda and disinformation to serve Proceed with caution: This website severely violates basic the Kremlin’s interests. standards of credibility and transparency. Score: 12.5/100 Ownership and Sputnik Deutschland is a subsidiary of Rossiya Financing Segodnya, a Russian government-owned international Does not repeatedly publish news agency. Rossiya Segodnya was established in false content (22points) December 2013 by Russian President Vladimir Putin. Gathers and presents The international broadcasting service, Voice of Russia, information responsibly (18) and the state-run news agency, RIA Novosti, were Regularly corrects or clarifies dissolved and merged into Rossiya Segodnya. errors (12.5) Rossiya Segodnya launched Sputnik in November Handles the difference between news and opinion responsibly 2014. Sputnik Deutschland also runs the radio station (12.5) SNA-Radio, which broadcasts in collaboration with the Avoids deceptive headlines (10) Bavarian radio station Mega Radio. Website discloses ownership The site runs advertisements. and financing (7.5) Clearly labels advertising (7.5) Content Sputnik Deutschland covers international politics, Reveals who's in charge, business, science, technology, culture, and celebrities. including any possible conflicts It has a separate section for German news, which of interest (5) primarily covers politics and major crime stories. The site provides names of content creators, along with The site states on its About Us (Über Uns) page that it either contact or biographical “reports on global politics and business only for information (5) audiences abroad.” Sputnik is headquartered in Moscow, has bureaus in 34 countries, and produces Criteria are listed in order of content in 30 languages.
    [Show full text]
  • Information to Users
    INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while ofriers may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. ProQuest Information and Learning 300 North Zeeb Road, Ann Arbor, Ml 48106-1346 USA 800-521-0600 UMT USING THE COGNITIVE APPRENTICESHIP MODEL TO TEACH ACTION RESEARCH TO PRESERVICE TEACHERS DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Georgene Rawding Risko, M.Ed. ***** The Ohio State University 2001 Dissertation Committee: Approved by Professor Marilyn Johnston, Adviser Professor Rebecca Kantor College of Education Professor Barbara Seidl UMI Number 3031256 UMI' UMI Microform 3031256 Copyright 2002 by Bell & Howell Information and Learning Company.
    [Show full text]
  • U.S. Government Printing Office Style Manual, 2008
    U.S. Government Printing Offi ce Style Manual An official guide to the form and style of Federal Government printing 2008 PPreliminary-CD.inddreliminary-CD.indd i 33/4/09/4/09 110:18:040:18:04 AAMM Production and Distribution Notes Th is publication was typeset electronically using Helvetica and Minion Pro typefaces. It was printed using vegetable oil-based ink on recycled paper containing 30% post consumer waste. Th e GPO Style Manual will be distributed to libraries in the Federal Depository Library Program. To fi nd a depository library near you, please go to the Federal depository library directory at http://catalog.gpo.gov/fdlpdir/public.jsp. Th e electronic text of this publication is available for public use free of charge at http://www.gpoaccess.gov/stylemanual/index.html. Use of ISBN Prefi x Th is is the offi cial U.S. Government edition of this publication and is herein identifi ed to certify its authenticity. ISBN 978–0–16–081813–4 is for U.S. Government Printing Offi ce offi cial editions only. Th e Superintendent of Documents of the U.S. Government Printing Offi ce requests that any re- printed edition be labeled clearly as a copy of the authentic work, and that a new ISBN be assigned. For sale by the Superintendent of Documents, U.S. Government Printing Office Internet: bookstore.gpo.gov Phone: toll free (866) 512-1800; DC area (202) 512-1800 Fax: (202) 512-2104 Mail: Stop IDCC, Washington, DC 20402-0001 ISBN 978-0-16-081813-4 (CD) II PPreliminary-CD.inddreliminary-CD.indd iiii 33/4/09/4/09 110:18:050:18:05 AAMM THE UNITED STATES GOVERNMENT PRINTING OFFICE STYLE MANUAL IS PUBLISHED UNDER THE DIRECTION AND AUTHORITY OF THE PUBLIC PRINTER OF THE UNITED STATES Robert C.
    [Show full text]
  • Public Service, Private Media: the Political Economy of The
    PUBLIC SERVICE, PRIVATE MEDIA: THE POLITICAL ECONOMY OF THE CABLE-SATELLITE PUBLIC AFFAIRS NETWORK (C-SPAN) by GLENN MICHAEL MORRIS A DISSERTATION Presented to the School of Journalism and Communication and the Graduate School of the University of Oregon in partial fulfillment of the requirements for the degree of Doctor of Philosophy June 2010 11 University of Oregon Graduate School Confirmation ofApproval and Acceptance of Dissertation prepared by: Glenn Morris Title: "Public Service, Private Media: The Political Economy ofthe Cable-Satellite Public Affairs Network (C-SPAN)." This dissertation has been accepted and approved in partial fulfillment ofthe requirements for the degree in the Department of Journalism and Communication by: Janet Wasko, Chairperson, Journalism and Communication Carl Bybee, Member, Journalism and Communication Gabriela Martinez, Member, Journalism and Communication John Foster, Outside Member, Sociology and Richard Linton, Vice President for Research and Graduate Studies/Dean ofthe Graduate School for the University of Oregon. June 14,2010 Original approval signatures are on file with the Graduate School and the University of Oregon Libraries. 111 © 2010 Glenn Michael Morris IV An Abstract of the Dissertation of Glenn Michael Morris for the degree of Doctor of Philosophy in the School of Journalism and Communication to be taken June 2010 Title: PUBLIC SERVICE, PRIVATE MEDIA: THE POLITICAL ECONOMY OF THE CABLE-SATELLITE PUBLIC AFFAIRS NETWORK (C-SPAN) Approved: _ Dr. Janet Wasko The Satellite-Cable Public Affairs Network (C-SPAN) is the only television outlet in the U.S. providing Congressional coverage. Scholars have studied the network's public affairs content and unedited "gavel-to-gavel" style of production that distinguish it from other television channels.
    [Show full text]
  • Russia's Information War Propaganda Or Counter-Propaganda?
    Briefing October 2016 Russia's information war Propaganda or counter-propaganda? SUMMARY In the West, Russia is more often seen as an instigator than a target of propaganda. However, Kremlin documents and Russian media argue that Russia itself faces serious threats from external information activity. Allegedly, such threats could undermine political stability in the country, curtail its international influence, and jeopardise traditional values. Moscow therefore argues that it needs to take defensive measures. The Kremlin claims that the USA and its allies are waging an information war against Russia. ISIL/Da'esh, which produces an increasing amount of Russian-language material, is another major adversary. At home, the Kremlin has clamped down on media and civil society to exclude external influences. Internationally, it has launched media weapons such as news channel RT to compete with Western media, and bankrolled pro-Russian NGOs. Critics of the Kremlin dispute its claims of a Western-led information war against Russia, and accuse it of conducting disinformation campaigns to justify aggression in neighbouring countries such as Ukraine. In terms of meeting their declared goal of protecting Russia from information threats, Russian measures have succeeded in excluding most foreign influences from the country. The international impact is more questionable; Russia's image is still very poor, but that does not mean that the Kremlin cannot defend what it sees as the country's external interests. In this briefing: Strategy and priorities Roles and responsibilities Adversaries Main tools Insights and criticism EPRS | European Parliamentary Research Service Author: Martin Russell Members' Research Service PE 589.810 EN EPRS Russia's information war: Propaganda or counter-propaganda? Definitions: 'information', 'information war', 'propaganda' Russian official texts refer to 'information' rather than to 'propaganda'.
    [Show full text]
  • The Fast Approval and Slow Rollout of Sputnik V: Why Is Russia's Vaccine
    Article The Fast Approval and Slow Rollout of Sputnik V: Why Is Russia’s Vaccine Rollout Slower than That of Other Nations? Elza Mikule 1,*,† , Tuuli Reissaar 1,† , Jennifer Villers 1,† , Alain Simplice Takoupo Penka 1,† , Alexander Temerev 2 and Liudmila Rozanova 2 1 Global Studies Institute, University of Geneva, 1205 Geneva, Switzerland; [email protected] (T.R.); [email protected] (J.V.); [email protected] (A.S.T.P.) 2 Institute of Global Health, University of Geneva, 1202 Geneva, Switzerland; [email protected] (A.T.); [email protected] (L.R.) * Correspondence: [email protected] † These authors contributed equally. Abstract: The emergence of the SARS-CoV-2 pandemic in the beginning of 2020 led to the deployment of enormous amounts of resources by different countries for vaccine development, and the Russian Federation was the first country in the world to approve a COVID-19 vaccine on 11 August 2020. In our research we sought to crystallize why the rollout of Sputnik V has been relatively slow considering that it was the first COVID-19 vaccine approved in the world. We looked at production capacity, at the number of vaccine doses domestically administered and internationally exported, and at vaccine hesitancy levels. By 6 May 2021, more first doses of Sputnik V had been administered Citation: Mikule, E.; Reissaar, T.; abroad than domestically, suggesting that limited production capacity was unlikely to be the main Villers, J.; Takoupo Penka, A.S.; reason behind the slow rollout. What remains unclear, however, is why Russia prioritized vaccine Temerev, A.; Rozanova, L.
    [Show full text]
  • The Army Lawyer (ISSN 0364-1287) Editor, Captain John E
    Headquarters, Demrtment of the Armv A d Department of the Army Pamphlet 27-50-276 November 1995 Table of Contents I Articles Tips and Observations from the Trial Bench: The Sequel ..................................................................................................................................................... 3 Colonel Gary 1. Holland Summary Judgment Motions in Discrimination Litigation: A Useful Tool or a Waste of Good Trees? .............................................................................. 12 Major James E. Macklin A Military Look into Space: The Ultimate High Ground ...................................................................................................................................................... 19 Major Dough S. Anderson USALSA Report ...... ............................................................................................................................................... ............ 29 United States Army &gal Services Agency Clerk of Court Notes ............................................................................................................................................................................................ 29 Courts-Martial Processing Times and Nonjudicial Punishment Rates Litigation Division Notes ................................................ ............................................. .................................................. -. 30 E Pluribus Unum-Maybe: The Ninth and Tenth Circuits Conflict on Bankruptcy Setoff Rights Involving
    [Show full text]
  • Dec06 POSTER 012207.Indd
    The National Cancer Institute at Frederick What is it? Where is it? Story on page 20. Outstanding Mentors WINTER 2006–2007 What Makes an Outstanding Mentor? IN THIS ISSUE by Maritta Perry Grau Encouraging Young Scientists 4 “The day you accept a student or postdoc, you become a mentor,” Science Today 6 said Dr. Andrew Byrd, chief of the Structural Biophysics Laboratory, Platinum Publications 8 Center for Cancer Research Poster People Profi le 10 (CCR), at NCI-Frederick. Dr. Byrd and two other CCR Christopher J. Michejda 11 colleagues were among 11 scientists that NCI recently Science Communications 12 recognized for their excellent mentoring skills. Dr. William Outreach and Special Programs 13 Farrar, head of the Cancer Stem Second from right, Dr. William Farrar, Head, Cell Section, Laboratory of Cancer Stem Cell Section, Laboratory of Protective Services 14 Cancer Prevention, received Cancer Prevention, CCR, with mentees. an Outstanding Mentor Award. Open Houses 15 Dr. Byrd and Dr. Alexander for their commitment to fostering the Wlodawer, chief, Macromolecular independent careers of their fellows, Books for Mongolia 16 Crystallography Laboratory, received students, and trainees. According to Frederick Employee Diversity Mentor of Merit awards. Dr. John Niederhuber, NCI director, Team 17 Created in 2001, the Mentoring “Each of this year’s Mentor honorees Awards recognize NCI investigators continued on page 2 New Faces at NCI-Frederick 18 Employee Assistance Program NCI-Frederick People 19 The Poster Puzzler 20 Employee Assistance Program
    [Show full text]
  • WP¥ Kllv Rrau' Dºlp]Q]¨OU. CT Yop¾v S\Lh|°¥¨V D¾qu Iuoflsjl, Oãoçvqoolp] Bwp V]J]Opu Ja¾lsjl Kla]Š
    F.Y. Reg. No. ....................................... 623623 March 2017 Name ............................................ Part - III ELECTRONICS Maximum : 60 Scores Time : 2 Hours Cool off time : 15 Minutes General Instructions to Candidates : z There is a 'Cool off time' of 15 minutes in addition to the writing time of 2 hrs. z You are neither allowed to write your answers nor to discuss anything with others during the 'cool off time'. z Use the 'cool off time' to get familiar with questions and to plan your answers. z Read the questions carefully before answering. z All questions are compulsory and only internal choice is allowed. z When you select a question, all the sub-questions must be answered from the same question itself. z Calculations, figures and graphs should be shown in the answer sheet itself. z Malayalam version of the questions is also provided. z Give equations wherever necessary. z Electronic devices except nonprogrammable calculators are not allowed in the Examination Hall. j]¡SÇw°¥: z j]¡Ç]xVa yop¾]jV kOrRo 15 o]j]ãV "WP¥ KLlV RRaU' DºLp]q]¨OU. CT yop¾V S\Lh|°¥¨V D¾qU IuOfLSjL, oãOçvqOoLp] Bwp v]j]opU ja¾LSjL kLa]Š. z D¾q°¥ IuOfOÐf]jV oOÒV S\Lh|°¥ èÈLkO¡‹U vLp]¨eU. z IŠL S\Lh|°¥¨OU D¾qU IuOfeU. z KqO S\Lh|jÒ¡ D¾qRouOfL¢ RfqR´aO¾O Wu]´L¤ Dk S\Lh|°tOU ASf S\Lh| jÒq]¤ j]ÐV fRÐ RfqR´aOS¨ºfLeV. z We¨V WPŸsOW¥, \]Nf°¥, NYLlOW¥, IÐ]v D¾qSkÕr]¤¾RÐ DºLp]q]¨eU. z Bvw|oOç òs¾V yovLW|°¥ RWLaO¨eU.
    [Show full text]
  • Of Bid to Clean , Fled from Officers
    :...a ' t X Zr ^ 6 / 't^A6B’hPmRTY-TW0 \ t ■' ^ W EDNESDAY, DECEMBER 4,'l967 Arcrage Daily Net Press Run r. For the Week Ended . , ThS Westlnr" -/■V November 30, IS67~ V ‘ Fanaaal at JB. S. Waatkor Bataa About Town 12,682 ' Fair, pioMw tealgW. iAw ULN Member o f the Audit Meapt S-lt aama niral araaa. Fadr, f^¥h#'CoiipI«« Club o f tho Second wanwir FHdajr. R ^ ' 46-M. XlbiixTegatlonal Chutph will bowl M&nthe»ter-^A City of rU^ge Charm ,:.»t the Oommiinlty Y Saturday DOUBLE S&H GREEH STAMPS WITH AtL CASH ; taiKht from 7:30 to 10 o’clock, Any VOL. LXXVII, NO. 56 Vouplea In the church are invited. (TWENTY-POUR PAGES—TWO SECTIONS)V MANCHESTER, CONN., -raURSDAt, 1>ECEMBER 6, 1957 . Thoae attending ehoul|d wear bowl- SALES THIS THURSDAY, DECEMBER 5! ICIaaolfled AdvartUliig a* Paga BS) PRICE FIVE tENTB ing ehoea. soft soled slippers or heavy socks.’Those planning to at- j tend should contact either Mrs. Harry Rylander Jr. or Mrs, James O h! Brakes? Irvtae, •fiBr on e etncl a .11 . ^December _ Los Angeles, De-:. 5 (ffi)—The Lakota Council. No. 01. D e^ee judge read the police report of Pocahontas, will meet tonight merry Christmas for everybody begins concerning a man before him^ at 7:30 in Odd Fellows Hall. Mem­ on a.recklesa driving change:' bers are asked to attend, as new of- right here! We've gifts for Mom, for Dad, for Sis "Driving 93 n.fles an hour fleers for the coming year will be Of Bid to Clean , fled from officers .
    [Show full text]
  • Innovations in PD
    PD Magazine Issue 8, Summer 2012 publicdiplomacymagaine.org Make an impact. EDITORIAL POLICY Public Diplomacy (PD) Magazine seeks contributions for each themed issue based on a structured solicitation system. Submission must be invited by the editorial board. Unsolicited articles will not be considered, or returned. Authors interested in contributing to PD should contact the editorial board about their proposals. Articles submitted to PD are reviewed by the editorial board, which is composed entirely of graduate students enrolled in the Masters of Public Diplomacy Public Diplomacy education at USC: program at the University of Southern California. Articles are evaluated based on relevance, originality, prose, and Two-year Master of Public Diplomacy (M.P.D.) argumentation. The editor-in-chief, in consultation with the editorial board, holds final authority for accepting or refusing submissions for publication. One-year Professional Master of Public Diplomacy Authors are responsible for ensuring the accuracy of their statements. The editorial staff will not conduct fact checks, but edit submissions for basic Mid-career Summer Institute in Public Diplomacy formatting and stylistic consistency only. Editors reserve the right to make changes for professional diplomats in accordance with PD style specifications. Copyright of published articles remains with Public Diplomacy (PD) Magazine. No article in its entirety or a part thereof may be published in any form without proper citation credit. ABOUT PD MAGAZINE Home of the USC Center on Public Diplomacy PD is a publication of the Asssociation of Public Diplomacy Scholars (APDS) at the Annenberg School, online at at the University of Southern California, with support from the USC Center on Public Diplomacy at the Annenberg School, USC College’s School of International Relations, www.uscpublicdiplomacy.com the Annenberg School for Communication and Journalism and USC Annenberg Press.
    [Show full text]