Hawaiian Shell News 26(2)
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Development of a Quantitative PCR Assay for the Detection And
bioRxiv preprint doi: https://doi.org/10.1101/544247; this version posted February 8, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Development of a quantitative PCR assay for the detection and enumeration of a potentially ciguatoxin-producing dinoflagellate, Gambierdiscus lapillus (Gonyaulacales, Dinophyceae). Key words:Ciguatera fish poisoning, Gambierdiscus lapillus, Quantitative PCR assay, Great Barrier Reef Kretzschmar, A.L.1,2, Verma, A.1, Kohli, G.S.1,3, Murray, S.A.1 1Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia 2ithree institute (i3), University of Technology Sydney, Ultimo, 2007 NSW, Australia, [email protected] 3Alfred Wegener-Institut Helmholtz-Zentrum fr Polar- und Meeresforschung, Am Handelshafen 12, 27570, Bremerhaven, Germany Abstract Ciguatera fish poisoning is an illness contracted through the ingestion of seafood containing ciguatoxins. It is prevalent in tropical regions worldwide, including in Australia. Ciguatoxins are produced by some species of Gambierdiscus. Therefore, screening of Gambierdiscus species identification through quantitative PCR (qPCR), along with the determination of species toxicity, can be useful in monitoring potential ciguatera risk in these regions. In Australia, the identity, distribution and abundance of ciguatoxin producing Gambierdiscus spp. is largely unknown. In this study we developed a rapid qPCR assay to quantify the presence and abundance of Gambierdiscus lapillus, a likely ciguatoxic species. We assessed the specificity and efficiency of the qPCR assay. The assay was tested on 25 environmental samples from the Heron Island reef in the southern Great Barrier Reef, a ciguatera endemic region, in triplicate to determine the presence and patchiness of these species across samples from Chnoospora sp., Padina sp. -
Mollusca, Neogastropoda) from the Mozambique Channel and New Caledonia
Bull. Mus. natn. Hist, nat., Paris, 4e ser., 3, 1981, section A, n° 4 : 985-1009. On a collection of buccinacean and mitracean Gastropods (Mollusca, Neogastropoda) from the Mozambique Channel and New Caledonia by W. 0. CERNOHORSKY Abstract. — The present paper deals with a collection of 59 species of buccinacean and mitra- cean gastropods belonging to 4 families from moderately shallow to deep water around the Mozam- bique Channel area, north of Madagascar. A total of 27 % of the species recovered are new geogra- phical range extensions. The New Caledonian material consists of 21 species belonging to 5 fami- lies, and was dredged, with one exception, in moderately deep water. A total of 38 % of the New Caledonian species represent new geographical records, and one of these is a new species : Voluto- mitra (Waimatea) vaubani n. sp. The new name Vexillum (Costellaria) duplex is proposed for the homonymous Mitra simphcissima Schepman, 1911, and its var. glabra Schepman, 1911. Résumé. — L'auteur étudie une collection de 59 espèces appartenant à 4 familles de Gasté- ropodes Buccinacea et Mitracea dragués dans le nord du canal du Mozambique, à des profondeurs diverses. L'étude montre une extension de l'aire de répartition connue pour 27 % des espèces. Le matériel néo-calédonien comprend 21 espèces appartenant à 5 familles et a été dragué, à une exception près, en eau relativement peu profonde. L'aire de répartition connue se trouve étendue pour 38 % des espèces, dont une est nouvelle : Volulomilra (Waimatea) vaubani n. sp. Le nom nouveau Vexillum (Costellaria) duplex est proposé en remplacement du nom Mitra simplicissima Schepman, 1911, et de sa variété glabra Schepman, 1911, tous deux préoccupés. -
MCR LTER 3Yr Report 2007 V
This document is a contribution of the Moorea Coral Reef LTER (OCE 04-17412) June 8, 2007 Schedule for MCR LTER Site Visit Sunday, July 8 Check into Sheraton Moorea Lagoon & Spa Afternoon Optional Moorea Terrestrial Tour 4:30-5:00 Gump Station Tour/Orientation 5:00-5:30 Snorkel Gear Setup for Site Team/Observers – Gump Dock 5:30-6:30 Welcome Cocktail – Gump House 6:30-7:30 Dinner – Gump Station 7:30-9:30 NSF Site Team Meeting – Gump Director’s Office 9:30 Return to Sheraton Monday, July 9 6:45 Pickup at Sheraton 7:00-7:45 Breakfast – Gump Station 7:45-11:45 Field Trip (Departing Gump Station; Review Team Dropped at Sheraton) 12:15 Pickup at Sheraton 12:30-1:30 Lunch – Gump Station 1:30-4:30 Research Talks – Library • MCR Programmatic Research Overview (Russ Schmitt) o Time Series Program (Andy Brooks) o Bio-Physical Coupling (Bob Carpenter) o Population & Community Dynamics (Sally Holbrook) o Coral Functional Biology (Pete Edmunds & Roger Nisbet) • Concluding Remarks (Russ Schmitt) 4:00-5:00 Grad Student/Post Doc Demonstrations – MCR Lab & Gump Wet Lab 5:00-6:30 Grad Student/Post Doc Hosted Poster Session/Cocktails – Library 6:30-7:30 Dinner – Gump Station 7:30 Return to Sheraton Tuesday, July 10 7:15 Pickup at Sheraton 7:30-8:30 Breakfast – Gump Station 8:30-12:00 Site Talks - Library • IM (Sabine Grabner) • Site Management & Institutional Relations (Russ Schmitt) • Education & Outreach (Michele Kissinger) • Network, Cross Site & International Activities (Sally Holbrook) 12:00-1:00 Lunch – Gump Station 1:00-5:30 Executive Session – Gump Director’s Office 5:30-6:45 Exit Interview - Library 6:45-9:30 Tahitian Feast & Dance Performance – Gump Station 9:30 Return to Sheraton Wednesday, July 11 7:00-8:00 Breakfast – Gump Station 8:00-12:00 Optional Field Trips 12:00-1:00 Lunch – Gump Station i ii Volume I. -
In Marine Protected Areas in Palawan, Philippines: Prospects for Conservation
Iranica Journal of Energy and Environment 7(2): 193-202, 2016 Iranica Journal of Energy & Environment Journal Homepage: www.ijee.net IJEE an official peer review journal of Babol Noshirvani University of Technology, ISSN:2079-2115 Spatial and Temporal Abundance of the reef gastropod Tectus niloticus (Gastropoda: Tegulidae) in Marine Protected Areas in Palawan, Philippines: Prospects for Conservation R. G. Dolorosa1*, A. Grant2, J. A. Gill3 1College of Fisheries and Aquatic Sciences, Western Philippines University, Philippines 2School of Environmental Sciences, University of East Anglia, UK 3School of Biological Sciences, University of East Anglia, UK ABSTRACT P A P E R I N F O The unsustainable harvesting of the reef gastropod Tectus niloticus or ‘trochus’ for the production of Paper history: ‘mother of pearl’ buttons have led to the collapse of its population and closure of its fishery in some Received 11 July 2015 countries. With the costly conservation measure involving the restocking of hatchery produced Accepted in revised form 12 December 2015 juveniles in partly protected reefs in the Philippines, this study assessed the abundance of trochus in three types of habitats of three Marine Protected Areas (MPAs) in Palawan, Philippines to document the status of its populations and to propose a more relevant conservation measure. Unguarded and Keywords: continuously exploited MPAs in the mainland Palawan harboured the least numbers of trochus. By Abundance contrast, in effectively protected areas of the Tubbataha Reefs Natural Park (TRNP), densities were Size structure quite high with large trochus being abundant in three types of habitats. However, the densities declined Protected area the farther the site from the Ranger Station of TRNP. -
As a Novel Vector of Ciguatera Poisoning: Detection of Pacific Ciguatoxins in Toxic Samples from Nuku Hiva Island (French Polynesia)
toxins Article Tectus niloticus (Tegulidae, Gastropod) as a Novel Vector of Ciguatera Poisoning: Detection of Pacific Ciguatoxins in Toxic Samples from Nuku Hiva Island (French Polynesia) Hélène Taiana Darius 1,*,† ID ,Mélanie Roué 2,† ID , Manoella Sibat 3 ID ,Jérôme Viallon 1, Clémence Mahana iti Gatti 1, Mark W. Vandersea 4, Patricia A. Tester 5, R. Wayne Litaker 4, Zouher Amzil 3 ID , Philipp Hess 3 ID and Mireille Chinain 1 1 Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae—UMR 241-EIO, P.O. Box 30, 98713 Papeete, Tahiti, French Polynesia; [email protected] (J.V.); [email protected] (C.M.i.G.); [email protected] (M.C.) 2 Institut de Recherche pour le Développement (IRD)—UMR 241-EIO, P.O. Box 529, 98713 Papeete, Tahiti, French Polynesia; [email protected] 3 IFREMER, Phycotoxins Laboratory, F-44311 Nantes, France; [email protected] (M.S.); [email protected] (Z.A.); [email protected] (P.H.) 4 National Oceanic and Atmospheric Administration, National Ocean Service, Centers for Coastal Ocean Science, Beaufort Laboratory, Beaufort, NC 28516, USA; [email protected] (M.W.V.); [email protected] (R.W.L.) 5 Ocean Tester, LLC, Beaufort, NC 28516, USA; [email protected] * Correspondence: [email protected]; Tel.: +689-40-416-484 † These authors contributed equally to this work. Received: 25 November 2017; Accepted: 18 December 2017; Published: 21 December 2017 Abstract: Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. -
(Gastropods and Bivalves) and Notes on Environmental Conditions of Two
Journal of Entomology and Zoology Studies 2014; 2 (5): 72-90 ISSN 2320-7078 The molluscan fauna (gastropods and bivalves) JEZS 2014; 2 (5): 72-90 © 2014 JEZS and notes on environmental conditions of two Received: 24-08-2014 Accepted: 19-09-2014 adjoining protected bays in Puerto Princesa City, Rafael M. Picardal Palawan, Philippines College of Fisheries and Aquatic Sciences, Western Philippines University Rafael M. Picardal and Roger G. Dolorosa Roger G. Dolorosa Abstract College of Fisheries and Aquatic Sciences, Western Philippines With the rising pressure of urbanization to biodiversity, this study aimed to obtain baseline information University on species richness of gastropods and bivalves in two protected bays (Turtle and Binunsalian) in Puerto Princesa City, Philippines before the establishment of the proposed mega resort facilities. A total of 108 species were recorded, (19 bivalves and 89 gastropods). The list includes two rare miters, seven recently described species and first record of Timoclea imbricata (Veneridae) in Palawan. Threatened species were not encountered during the survey suggesting that both bays had been overfished. Turtle Bay had very low visibility, low coral cover, substantial signs of ecosystem disturbances and shift from coral to algal communities. Although Binunsalian Bay had clearer waters and relatively high coral cover, associated fish and macrobenthic invertebrates were of low or no commercial values. Upon the establishment and operations of the resort facilities, follow-up species inventories and habitat assessment are suggested to evaluate the importance of private resorts in biodiversity restoration. Keywords: Binunsalian Bay, bivalves, gastropods, Palawan, species inventory, Turtle Bay 1. Introduction Gastropods and bivalves are among the most fascinating groups of molluscs that for centuries have attracted hobbyists, businessmen, ecologists and scientists among others from around the globe. -
UC Davis UC Davis Previously Published Works
UC Davis UC Davis Previously Published Works Title Molluscan marginalia: Serration at the lip edge in gastropods Permalink https://escholarship.org/uc/item/2mx5c6w9 Journal Journal of Molluscan Studies, 80(3) ISSN 0260-1230 Author Vermeij, GJ Publication Date 2014 DOI 10.1093/mollus/eyu020 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Journal of The Malacological Society of London Molluscan Studies Journal of Molluscan Studies (2014) 80: 326–336. doi:10.1093/mollus/eyu020 Advance Access publication date: 16 April 2014 Molluscan marginalia: serration at the lip edge in gastropods Geerat J. Vermeij Geology Department, University of California, One Shields Avenue, Davis, CA 95616, USA Correspondence: G.J. Vermeij; e-mail: [email protected] Downloaded from (Received 5 September 2013; accepted 10 February 2014) ABSTRACT The shells of many marine gastropods have ventrally directed serrations (serial projections) at the edge http://mollus.oxfordjournals.org/ of the adult outer lip. These poorly studied projections arise as extensions either of external spiral cords or of interspaces between cords. This paper describes taxonomic, phylogenetic, architectural and func- tional aspects of serrations. Cord-associated serrations occur in cerithiids, strombids, the personid Distorsio anus, ocenebrine muricids and some cancellariids. Interspace-associated serrations are phylo- genetically much more widespread, and occur in at least 16 family-level groups. The nature of serration may be taxonomically informative in some fissurellids, littorinids, strombids and costellariids, among other groups. Serrated outer lips occur only in gastropods in which the apex points more backward than upward, but the presence of serrations is not a necessary byproduct of the formation of spiral sculp- tural elements. -
Prioritized Species for Mariculture in India
Prioritized Species for Mariculture in India Compiled & Edited by Ritesh Ranjan Muktha M Shubhadeep Ghosh A Gopalakrishnan G Gopakumar Imelda Joseph ICAR - Central Marine Fisheries Research Institute Post Box No. 1603, Ernakulam North P.O. Kochi – 682 018, Kerala, India www.cmfri.org.in 2017 Prioritized Species for Mariculture in India Published by: Dr. A Gopalakrishnan Director ICAR - Central Marine Fisheries Research Institute Post Box No. 1603, Ernakulam North P.O. Kochi – 682 018, Kerala, India www.cmfri.org.in Email: [email protected] Tel. No.: +91-0484-2394867 Fax No.: +91-0484-2394909 Designed at G.K. Print House Pvt. Ltd. Rednam Gardens Visakhapatnam- 530002, Andhra Pradesh Cell: +91 9848196095, www.gkprinthouse.com Cover page design: Abhilash P. R., CMFRI, Kochi Illustrations: David K. M., CMFRI, Kochi Publication, Production & Co-ordination: Library & Documentation Centre, CMFRI Printed on: November 2017 ISBN 978-93-82263-14-2 © 2017 ICAR - Central Marine Fisheries Research Institute, Kochi All rights reserved. Material contained in this publication may not be reproduced in any form without the permission of the publisher. Citation : Ranjan, R., Muktha, M., Ghosh, S., Gopalakrishnan, A., Gopakumar, G. and Joseph, I. (Eds.). 2017. Prioritized Species for Mariculture in India. ICAR-CMFRI, Kochi. 450 pp. CONTENTS Foreword ................................................................................................................. i Preface ................................................................................................................. -
128 Freiberg, 2012 Protoconch Characters of Late Cretaceous
Freiberger Forschungshefte, C 542 psf (20) 93 – 128 Freiberg, 2012 Protoconch characters of Late Cretaceous Latrogastropoda (Neogastropoda and Neomesogastropoda) as an aid in the reconstruction of the phylogeny of the Neogastropoda by Klaus Bandel, Hamburg & David T. Dockery III, Jackson with 5 plates BANDEL, K. & DOCKERY, D.T. III (2012): Protoconch characters of Late Cretaceous Latrogastropoda (Neogastropoda and Neomesogastropoda) as an aid in the reconstruction of the phylogeny of the Neogastropoda. Paläontologie, Stratigraphie, Fazies (20), Freiberger Forschungshefte, C 542: 93–128; Freiberg. Keywords: Latrogastropoda, Neogastropoda, Neomesogastropoda, Cretaceous. Addresses: Prof. Dr. Klaus Bandel, Universitat Hamburg, Geologisch Paläontologisches Institut und Museum, Bundesstrasse 55, D-20146 Hamburg, email: [email protected]; David T. Dockery III, Mississippi Department of Environmental Quality, Office of Geology, P.O. Box 20307, 39289-1307 Jackson, MS, 39289- 1307, U.S.A., email: [email protected]. Contents: Abstract Zusammenfassung 1 Introduction 2 Palaeontology 3 Discussion 3.1 Characters of protoconch morphology among Muricoidea 3.2 Characteristics of the protoconch of Buccinidae, Nassariidae, Columbellinidae and Mitridae 3.3 Characteristics of the protoconch morphology among Toxoglossa References Abstract Late Cretaceous Naticidae, Cypraeidae and Calyptraeidae can be recognized by the shape of their teleoconch, as well as by their characteristic protoconch morphology. The stem group from which the Latrogastropoda originated lived during or shortly before Aptian/Albian time (100–125 Ma). Several groups of Latrogastropoda that lived at the time of deposition of the Campanian to Maastrichtian (65–83 Ma) Ripley Formation have no recognized living counterparts. These Late Cretaceous species include the Sarganoidea, with the families Sarganidae, Weeksiidae and Moreidae, which have a rounded and low protoconch with a large embryonic whorl. -
Identification of Shell Colour Pigments in Marine Snails Clanculus Pharaonius and C
RESEARCH ARTICLE Identification of Shell Colour Pigments in Marine Snails Clanculus pharaonius and C. margaritarius (Trochoidea; Gastropoda) S. T. Williams1*, S. Ito2, K. Wakamatsu2, T. Goral3, N. P. Edwards4, R. A. Wogelius4, T. Henkel4, L. F. C. de Oliveira5, L. F. Maia5, S. Strekopytov3, T. Jeffries3†, D. I. Speiser6,J. T. Marsden7 1 Natural History Museum, Department of Life Sciences, London, United Kingdom, 2 Department of Chemistry, Fujita Health University School of Health Sciences, 1–98 Dengakugakubo, Kutsukake-cho, a11111 Toyoake, Aichi, Japan, 3 Natural History Museum, Imaging and Analysis Centre, London, United Kingdom, 4 School of Earth, Atmospheric, and Environmental Sciences, University of Manchester, Manchester, United Kingdom, 5 NEEM Núcleo de Espectroscopia e Estrutura Molecular, Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil, 6 Department of Biological Sciences, University of South Carolina, Columbia, South Carolina, United States of America, 7 Viapath, Reference Biochemistry Laboratories, King's College Hospital, London, United Kingdom † Deceased. OPEN ACCESS * [email protected] Citation: Williams ST, Ito S, Wakamatsu K, Goral T, Edwards NP, Wogelius RA, et al. (2016) Identification of Shell Colour Pigments in Marine Snails Clanculus Abstract pharaonius and C. margaritarius (Trochoidea; Gastropoda). PLoS ONE 11(7): e0156664. Colour and pattern are key traits with important roles in camouflage, warning and attraction. doi:10.1371/journal.pone.0156664 Ideally, in order to begin to understand the evolution and ecology of colour in nature, it is Editor: Geerat J. Vermeij, University of California, important to identify and, where possible, fully characterise pigments using biochemical UNITED STATES methods. -
Tectus (Trochus) Niloticus Search for Suitable Habitats Can Cause Equivocal Benefits of Protection in Village-Based Marine Reserves
RESEARCH ARTICLE Tectus (Trochus) niloticus search for suitable habitats can cause equivocal benefits of protection in village-based marine reserves Pascal Dumas1,2*, Jayven Ham3, Rocky Kaku3, Andrew William3, Jeremie Kaltavara3, Sompert Gereva3, Marc LeÂopold1,2 1 IRD, UMR 9220 ENTROPIE, NoumeÂa, Nouvelle-CaleÂdonie, 2 Laboratoire d'Excellence LABEX Corail, Perpignan, France, 3 Fisheries Department of Vanuatu, Port-Vila, Vanuatu a1111111111 * [email protected] a1111111111 a1111111111 a1111111111 a1111111111 Abstract In the Pacific, the protection of coral reef resources is often achieved through the implemen- tation of village-based marine reserves (VBMRs). While substantial fisheries benefits are often reported, results of quantitative approaches are controversial for benthic macroinver- OPEN ACCESS tebrates, whose life history traits may cause low congruence with protective measures Citation: Dumas P, Ham J, Kaku R, William A, implemented at non-ecologically relevant scales. This study investigated the structural and Kaltavara J, Gereva S, et al. (2017) Tectus behavioral responses of the exploited topshell Tectus niloticus within a very small (0.2 km2) (Trochus) niloticus search for suitable habitats can cause equivocal benefits of protection in village- VBMR in Vanuatu, south Pacific. The results of underwater surveys and a nine-month tag- based marine reserves. PLoS ONE 12(5): ging experiment emphasized contrasted, scale-dependent responses. At the reserve scale, e0176922. https://doi.org/10.1371/journal. our results failed to demonstrate any positive effect of protection after three years of closure. pone.0176922 In contrast, abundance, density and biomass increased more than ten-fold in the southern Editor: Maura (Gee) Geraldine Chapman, University part of the reserve, along with significantly larger (25%) individual sizes. -
Le Dispositif Ultramarin De L'ifremer
1 /2 OUTRE-MER : ACTIVITÉS SCIENTIFIQUES DE L’IFREMER JUIN 2019 LE DISPOSITIF ULTRAMARIN DE L’IFREMER Saint-Pierre et Miquelon Brest Martinique Guyane française Polynésie française La Réunion Nouvelle-Calédonie LE DISPOSITIF Siège IMPLANTÉ OUTRE-MER Laboratoire ultramarin de l'Ifremer Les laboratoires ultramarins de l’Ifremer sont présents Ces laboratoires ultramarins impliquent une centaine dans les trois grands océans : dans l’océan Pacifi que de salariés de l’Ifremer dont environ cinquante en Polynésie française et en Nouvelle-Calédonie ; permanents sur site. Ils accueillent également des dans l’océan Indien à l’Ile de La Réunion ; dans l’océan chercheurs, ingénieurs et techniciens de nombreux Atlantique, en Martinique, en Guyane française laboratoires métropolitains qui viennent y eff ectuer et à Saint-Pierre et Miquelon (SPM). Ces laboratoires des missions de plus ou moins longue durée. sont naturellement portés à étudier des questions De nombreux équipements remarquables y ont été scientifi ques d’intérêt local ; ce sont aussi autant implantés : des plateformes aquacoles (Martinique, de portes d'entrée pour observer les océans et mener Polynésie française et Nouvelle Calédonie) et des des recherches à l’échelle de notre planète. systèmes d’observation et de surveillance du milieu côtier et des lagons (Martinique, La Réunion). 2 /2 OUTRE-MER : ACTIVITÉS SCIENTIFIQUES DE L’IFREMER JUIN 2019 1 /3 OUTRE-MER : ACTIVITÉS SCIENTIFIQUES DE L’IFREMER JUIN 2019 EFFECTIFS AU 31 MARS 2019 Implantations Personnels permanents Personnels temporaires