Le Dispositif Ultramarin De L'ifremer
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Development of a Quantitative PCR Assay for the Detection And
bioRxiv preprint doi: https://doi.org/10.1101/544247; this version posted February 8, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Development of a quantitative PCR assay for the detection and enumeration of a potentially ciguatoxin-producing dinoflagellate, Gambierdiscus lapillus (Gonyaulacales, Dinophyceae). Key words:Ciguatera fish poisoning, Gambierdiscus lapillus, Quantitative PCR assay, Great Barrier Reef Kretzschmar, A.L.1,2, Verma, A.1, Kohli, G.S.1,3, Murray, S.A.1 1Climate Change Cluster (C3), University of Technology Sydney, Ultimo, 2007 NSW, Australia 2ithree institute (i3), University of Technology Sydney, Ultimo, 2007 NSW, Australia, [email protected] 3Alfred Wegener-Institut Helmholtz-Zentrum fr Polar- und Meeresforschung, Am Handelshafen 12, 27570, Bremerhaven, Germany Abstract Ciguatera fish poisoning is an illness contracted through the ingestion of seafood containing ciguatoxins. It is prevalent in tropical regions worldwide, including in Australia. Ciguatoxins are produced by some species of Gambierdiscus. Therefore, screening of Gambierdiscus species identification through quantitative PCR (qPCR), along with the determination of species toxicity, can be useful in monitoring potential ciguatera risk in these regions. In Australia, the identity, distribution and abundance of ciguatoxin producing Gambierdiscus spp. is largely unknown. In this study we developed a rapid qPCR assay to quantify the presence and abundance of Gambierdiscus lapillus, a likely ciguatoxic species. We assessed the specificity and efficiency of the qPCR assay. The assay was tested on 25 environmental samples from the Heron Island reef in the southern Great Barrier Reef, a ciguatera endemic region, in triplicate to determine the presence and patchiness of these species across samples from Chnoospora sp., Padina sp. -
MCR LTER 3Yr Report 2007 V
This document is a contribution of the Moorea Coral Reef LTER (OCE 04-17412) June 8, 2007 Schedule for MCR LTER Site Visit Sunday, July 8 Check into Sheraton Moorea Lagoon & Spa Afternoon Optional Moorea Terrestrial Tour 4:30-5:00 Gump Station Tour/Orientation 5:00-5:30 Snorkel Gear Setup for Site Team/Observers – Gump Dock 5:30-6:30 Welcome Cocktail – Gump House 6:30-7:30 Dinner – Gump Station 7:30-9:30 NSF Site Team Meeting – Gump Director’s Office 9:30 Return to Sheraton Monday, July 9 6:45 Pickup at Sheraton 7:00-7:45 Breakfast – Gump Station 7:45-11:45 Field Trip (Departing Gump Station; Review Team Dropped at Sheraton) 12:15 Pickup at Sheraton 12:30-1:30 Lunch – Gump Station 1:30-4:30 Research Talks – Library • MCR Programmatic Research Overview (Russ Schmitt) o Time Series Program (Andy Brooks) o Bio-Physical Coupling (Bob Carpenter) o Population & Community Dynamics (Sally Holbrook) o Coral Functional Biology (Pete Edmunds & Roger Nisbet) • Concluding Remarks (Russ Schmitt) 4:00-5:00 Grad Student/Post Doc Demonstrations – MCR Lab & Gump Wet Lab 5:00-6:30 Grad Student/Post Doc Hosted Poster Session/Cocktails – Library 6:30-7:30 Dinner – Gump Station 7:30 Return to Sheraton Tuesday, July 10 7:15 Pickup at Sheraton 7:30-8:30 Breakfast – Gump Station 8:30-12:00 Site Talks - Library • IM (Sabine Grabner) • Site Management & Institutional Relations (Russ Schmitt) • Education & Outreach (Michele Kissinger) • Network, Cross Site & International Activities (Sally Holbrook) 12:00-1:00 Lunch – Gump Station 1:00-5:30 Executive Session – Gump Director’s Office 5:30-6:45 Exit Interview - Library 6:45-9:30 Tahitian Feast & Dance Performance – Gump Station 9:30 Return to Sheraton Wednesday, July 11 7:00-8:00 Breakfast – Gump Station 8:00-12:00 Optional Field Trips 12:00-1:00 Lunch – Gump Station i ii Volume I. -
In Marine Protected Areas in Palawan, Philippines: Prospects for Conservation
Iranica Journal of Energy and Environment 7(2): 193-202, 2016 Iranica Journal of Energy & Environment Journal Homepage: www.ijee.net IJEE an official peer review journal of Babol Noshirvani University of Technology, ISSN:2079-2115 Spatial and Temporal Abundance of the reef gastropod Tectus niloticus (Gastropoda: Tegulidae) in Marine Protected Areas in Palawan, Philippines: Prospects for Conservation R. G. Dolorosa1*, A. Grant2, J. A. Gill3 1College of Fisheries and Aquatic Sciences, Western Philippines University, Philippines 2School of Environmental Sciences, University of East Anglia, UK 3School of Biological Sciences, University of East Anglia, UK ABSTRACT P A P E R I N F O The unsustainable harvesting of the reef gastropod Tectus niloticus or ‘trochus’ for the production of Paper history: ‘mother of pearl’ buttons have led to the collapse of its population and closure of its fishery in some Received 11 July 2015 countries. With the costly conservation measure involving the restocking of hatchery produced Accepted in revised form 12 December 2015 juveniles in partly protected reefs in the Philippines, this study assessed the abundance of trochus in three types of habitats of three Marine Protected Areas (MPAs) in Palawan, Philippines to document the status of its populations and to propose a more relevant conservation measure. Unguarded and Keywords: continuously exploited MPAs in the mainland Palawan harboured the least numbers of trochus. By Abundance contrast, in effectively protected areas of the Tubbataha Reefs Natural Park (TRNP), densities were Size structure quite high with large trochus being abundant in three types of habitats. However, the densities declined Protected area the farther the site from the Ranger Station of TRNP. -
As a Novel Vector of Ciguatera Poisoning: Detection of Pacific Ciguatoxins in Toxic Samples from Nuku Hiva Island (French Polynesia)
toxins Article Tectus niloticus (Tegulidae, Gastropod) as a Novel Vector of Ciguatera Poisoning: Detection of Pacific Ciguatoxins in Toxic Samples from Nuku Hiva Island (French Polynesia) Hélène Taiana Darius 1,*,† ID ,Mélanie Roué 2,† ID , Manoella Sibat 3 ID ,Jérôme Viallon 1, Clémence Mahana iti Gatti 1, Mark W. Vandersea 4, Patricia A. Tester 5, R. Wayne Litaker 4, Zouher Amzil 3 ID , Philipp Hess 3 ID and Mireille Chinain 1 1 Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae—UMR 241-EIO, P.O. Box 30, 98713 Papeete, Tahiti, French Polynesia; [email protected] (J.V.); [email protected] (C.M.i.G.); [email protected] (M.C.) 2 Institut de Recherche pour le Développement (IRD)—UMR 241-EIO, P.O. Box 529, 98713 Papeete, Tahiti, French Polynesia; [email protected] 3 IFREMER, Phycotoxins Laboratory, F-44311 Nantes, France; [email protected] (M.S.); [email protected] (Z.A.); [email protected] (P.H.) 4 National Oceanic and Atmospheric Administration, National Ocean Service, Centers for Coastal Ocean Science, Beaufort Laboratory, Beaufort, NC 28516, USA; [email protected] (M.W.V.); [email protected] (R.W.L.) 5 Ocean Tester, LLC, Beaufort, NC 28516, USA; [email protected] * Correspondence: [email protected]; Tel.: +689-40-416-484 † These authors contributed equally to this work. Received: 25 November 2017; Accepted: 18 December 2017; Published: 21 December 2017 Abstract: Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. -
(Gastropods and Bivalves) and Notes on Environmental Conditions of Two
Journal of Entomology and Zoology Studies 2014; 2 (5): 72-90 ISSN 2320-7078 The molluscan fauna (gastropods and bivalves) JEZS 2014; 2 (5): 72-90 © 2014 JEZS and notes on environmental conditions of two Received: 24-08-2014 Accepted: 19-09-2014 adjoining protected bays in Puerto Princesa City, Rafael M. Picardal Palawan, Philippines College of Fisheries and Aquatic Sciences, Western Philippines University Rafael M. Picardal and Roger G. Dolorosa Roger G. Dolorosa Abstract College of Fisheries and Aquatic Sciences, Western Philippines With the rising pressure of urbanization to biodiversity, this study aimed to obtain baseline information University on species richness of gastropods and bivalves in two protected bays (Turtle and Binunsalian) in Puerto Princesa City, Philippines before the establishment of the proposed mega resort facilities. A total of 108 species were recorded, (19 bivalves and 89 gastropods). The list includes two rare miters, seven recently described species and first record of Timoclea imbricata (Veneridae) in Palawan. Threatened species were not encountered during the survey suggesting that both bays had been overfished. Turtle Bay had very low visibility, low coral cover, substantial signs of ecosystem disturbances and shift from coral to algal communities. Although Binunsalian Bay had clearer waters and relatively high coral cover, associated fish and macrobenthic invertebrates were of low or no commercial values. Upon the establishment and operations of the resort facilities, follow-up species inventories and habitat assessment are suggested to evaluate the importance of private resorts in biodiversity restoration. Keywords: Binunsalian Bay, bivalves, gastropods, Palawan, species inventory, Turtle Bay 1. Introduction Gastropods and bivalves are among the most fascinating groups of molluscs that for centuries have attracted hobbyists, businessmen, ecologists and scientists among others from around the globe. -
Prioritized Species for Mariculture in India
Prioritized Species for Mariculture in India Compiled & Edited by Ritesh Ranjan Muktha M Shubhadeep Ghosh A Gopalakrishnan G Gopakumar Imelda Joseph ICAR - Central Marine Fisheries Research Institute Post Box No. 1603, Ernakulam North P.O. Kochi – 682 018, Kerala, India www.cmfri.org.in 2017 Prioritized Species for Mariculture in India Published by: Dr. A Gopalakrishnan Director ICAR - Central Marine Fisheries Research Institute Post Box No. 1603, Ernakulam North P.O. Kochi – 682 018, Kerala, India www.cmfri.org.in Email: [email protected] Tel. No.: +91-0484-2394867 Fax No.: +91-0484-2394909 Designed at G.K. Print House Pvt. Ltd. Rednam Gardens Visakhapatnam- 530002, Andhra Pradesh Cell: +91 9848196095, www.gkprinthouse.com Cover page design: Abhilash P. R., CMFRI, Kochi Illustrations: David K. M., CMFRI, Kochi Publication, Production & Co-ordination: Library & Documentation Centre, CMFRI Printed on: November 2017 ISBN 978-93-82263-14-2 © 2017 ICAR - Central Marine Fisheries Research Institute, Kochi All rights reserved. Material contained in this publication may not be reproduced in any form without the permission of the publisher. Citation : Ranjan, R., Muktha, M., Ghosh, S., Gopalakrishnan, A., Gopakumar, G. and Joseph, I. (Eds.). 2017. Prioritized Species for Mariculture in India. ICAR-CMFRI, Kochi. 450 pp. CONTENTS Foreword ................................................................................................................. i Preface ................................................................................................................. -
Identification of Shell Colour Pigments in Marine Snails Clanculus Pharaonius and C
RESEARCH ARTICLE Identification of Shell Colour Pigments in Marine Snails Clanculus pharaonius and C. margaritarius (Trochoidea; Gastropoda) S. T. Williams1*, S. Ito2, K. Wakamatsu2, T. Goral3, N. P. Edwards4, R. A. Wogelius4, T. Henkel4, L. F. C. de Oliveira5, L. F. Maia5, S. Strekopytov3, T. Jeffries3†, D. I. Speiser6,J. T. Marsden7 1 Natural History Museum, Department of Life Sciences, London, United Kingdom, 2 Department of Chemistry, Fujita Health University School of Health Sciences, 1–98 Dengakugakubo, Kutsukake-cho, a11111 Toyoake, Aichi, Japan, 3 Natural History Museum, Imaging and Analysis Centre, London, United Kingdom, 4 School of Earth, Atmospheric, and Environmental Sciences, University of Manchester, Manchester, United Kingdom, 5 NEEM Núcleo de Espectroscopia e Estrutura Molecular, Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil, 6 Department of Biological Sciences, University of South Carolina, Columbia, South Carolina, United States of America, 7 Viapath, Reference Biochemistry Laboratories, King's College Hospital, London, United Kingdom † Deceased. OPEN ACCESS * [email protected] Citation: Williams ST, Ito S, Wakamatsu K, Goral T, Edwards NP, Wogelius RA, et al. (2016) Identification of Shell Colour Pigments in Marine Snails Clanculus Abstract pharaonius and C. margaritarius (Trochoidea; Gastropoda). PLoS ONE 11(7): e0156664. Colour and pattern are key traits with important roles in camouflage, warning and attraction. doi:10.1371/journal.pone.0156664 Ideally, in order to begin to understand the evolution and ecology of colour in nature, it is Editor: Geerat J. Vermeij, University of California, important to identify and, where possible, fully characterise pigments using biochemical UNITED STATES methods. -
Tectus (Trochus) Niloticus Search for Suitable Habitats Can Cause Equivocal Benefits of Protection in Village-Based Marine Reserves
RESEARCH ARTICLE Tectus (Trochus) niloticus search for suitable habitats can cause equivocal benefits of protection in village-based marine reserves Pascal Dumas1,2*, Jayven Ham3, Rocky Kaku3, Andrew William3, Jeremie Kaltavara3, Sompert Gereva3, Marc LeÂopold1,2 1 IRD, UMR 9220 ENTROPIE, NoumeÂa, Nouvelle-CaleÂdonie, 2 Laboratoire d'Excellence LABEX Corail, Perpignan, France, 3 Fisheries Department of Vanuatu, Port-Vila, Vanuatu a1111111111 * [email protected] a1111111111 a1111111111 a1111111111 a1111111111 Abstract In the Pacific, the protection of coral reef resources is often achieved through the implemen- tation of village-based marine reserves (VBMRs). While substantial fisheries benefits are often reported, results of quantitative approaches are controversial for benthic macroinver- OPEN ACCESS tebrates, whose life history traits may cause low congruence with protective measures Citation: Dumas P, Ham J, Kaku R, William A, implemented at non-ecologically relevant scales. This study investigated the structural and Kaltavara J, Gereva S, et al. (2017) Tectus behavioral responses of the exploited topshell Tectus niloticus within a very small (0.2 km2) (Trochus) niloticus search for suitable habitats can cause equivocal benefits of protection in village- VBMR in Vanuatu, south Pacific. The results of underwater surveys and a nine-month tag- based marine reserves. PLoS ONE 12(5): ging experiment emphasized contrasted, scale-dependent responses. At the reserve scale, e0176922. https://doi.org/10.1371/journal. our results failed to demonstrate any positive effect of protection after three years of closure. pone.0176922 In contrast, abundance, density and biomass increased more than ten-fold in the southern Editor: Maura (Gee) Geraldine Chapman, University part of the reserve, along with significantly larger (25%) individual sizes. -
Growth, Movement and Recovery of Tagged Topshell, Trochus Niloticus, Juveniles in Imondayon, Anda, Pangasinan, Northern Philippi
International Journal of Fauna and Biological Studies 2018; 5(1): 01-04 E-ISSN: 2347-2677 P-ISSN: 2394-0522 IJFBS 2018; 5(1): 01-04 Growth, movement and recovery of tagged topshell, Received: 01-11-2017 Trochus niloticus, juveniles in Imondayon, Anda, Accepted: 02-12-2017 Pangasinan, Northern Philippines Emmanuel C Capinpin Jr Associate Professor IV, Department of Fisheries Science, Pangasinan State University – Emmanuel C Capinpin Jr Binmaley Campus, Binmaley, Pangasinan, Philippines Abstract In the present study, the growth, movement and recovery rates of tagged wild Trochus niloticus juveniles were assessed after deployment in Imondayon, Anda, Pangasinan, in Northern Philippines. Two batches composed of 51 (35.88 mm shell diameter ± 3.60) and 35 (36.56 mm ± 3.73) juveniles were tagged and released into an intertidal flat with coral rubble near a sand bar in Imondayon reef on November 27, 2014 and December 18, 2014, respectively. In the first batch, only 5 live specimens were retrieved after 89 days (9.80% recovery rate). On the other hand, in the second batch a low recovery rate of 5.88% was observed after 68 days. Observed growth rates in both batches ranged from 0.052 to 0.096 mm/day, which is similar to that observed in other previous studies on same-sized juveniles. Some shells were also recovered dead but whole and with observed growth, which suggests predation by non-crushing predatory gastropods and/or hermit crabs. Wild trochus are cryptic and highly mobile, moving into deeper areas as they grow in size, which also explains the low recovery rates observed in the present study. -
Checklist of the Mollusca of Cocos (Keeling) / Christmas Island Ecoregion
RAFFLES BULLETIN OF ZOOLOGY 2014 RAFFLES BULLETIN OF ZOOLOGY Supplement No. 30: 313–375 Date of publication: 25 December 2014 http://zoobank.org/urn:lsid:zoobank.org:pub:52341BDF-BF85-42A3-B1E9-44DADC011634 Checklist of the Mollusca of Cocos (Keeling) / Christmas Island ecoregion Siong Kiat Tan* & Martyn E. Y. Low Abstract. An annotated checklist of the Mollusca from the Australian Indian Ocean Territories (IOT) of Christmas Island (Indian Ocean) and the Cocos (Keeling) Islands is presented. The checklist combines data from all previous studies and new material collected during the recent Christmas Island Expeditions organised by the Lee Kong Chian Natural History Museum (formerly the Raffles Museum of Biodiversty Resarch), Singapore. The checklist provides an overview of the diversity of the malacofauna occurring in the Cocos (Keeling) / Christmas Island ecoregion. A total of 1,178 species representing 165 families are documented, with 760 (in 130 families) and 757 (in 126 families) species recorded from Christmas Island and the Cocos (Keeling) Islands, respectively. Forty-five species (or 3.8%) of these species are endemic to the Australian IOT. Fifty-seven molluscan records for this ecoregion are herein published for the first time. We also briefly discuss historical patterns of discovery and endemism in the malacofauna of the Australian IOT. Key words. Mollusca, Polyplacophora, Bivalvia, Gastropoda, Christmas Island, Cocos (Keeling) Islands, Indian Ocean INTRODUCTION The Cocos (Keeling) Islands, which comprise North Keeling Island (a single island atoll) and the South Keeling Christmas Island (Indian Ocean) (hereafter CI) and the Cocos Islands (an atoll consisting of more than 20 islets including (Keeling) Islands (hereafter CK) comprise the Australian Horsburgh Island, West Island, Direction Island, Home Indian Ocean Territories (IOT). -
Hawaiian Shell News 26(2)
AN EDUCATIONAL PUBLICATION OF THE HAWAIIAN MALACOLOGICAL SOCIETY VOL. XXVI NO.2 FEBRUARY, 1978 NEW SERIES NO. 218 SOCIETY FINANCES SHOWIMPROVEMENT 3 Financially, the Hawaiian Malacological So- ciety is alive and well in 1978,according to figures submitted by HMS Treasurer Wes Thorsson at the January meeting of the Board of Directors. "1977 was a good year for the Society," he said. "On December 31 our membership stood at 1506, and as of that date 1017 had renewed for 1978. Normally, memberships come in at a snail's pace and usually we do not have this large a percentage of renewals by January I. "The Society's receipts during the year came to $39,125. Of this, 67 per cent came from dues and sales of HSN back issues. Twenty-three per cent was from HSN advertising, three per cent from sales of books to members, four per cent rep- resented interest on savings accounts, and the , remaining three per cent was from miscellaneous sources. i "Expenditures totalled $35,488,of which 64 per I cent went to produce Hawaiian Shell News i (including reproduction of some back issues). Postage for HSN took thirteen per cent, and the same percentage went for office support for HSN. Purchase of books for members took two per cent, L- Swainsonia bic%r :.." ...~.~...~-_0.'""_";; S. casta local meetings and library maintenance took two per cent, mounting of the 1977 HMS Shell Show cost one per cent. The remaining three per cent by LEN HILL was for taxes, insurance, science fair awards and HASTINGS, Neb.- Three Indo-Pacific miter Swainsonia newcombii Pease, 1869 is found other miscellaneous expenses. -
Bivalve Mollusc Culture, Research in Thail"And
T ~ SH ~ ~ 207 ICLARM Technical Reports'19 - TR4 #19 c.1 I ,Bivalve Mollusc Culture, ,-I.. I . Research in Thail"and i} I Edited by " ~, E.W. McCoy . ~. Tanittha Chongpeepien , /I I l- \ .6' r\.~/ .' "" " ,' " - r, " . ",," t ~ , ~ "'! r---1 e.~!'-"~ f - .. ~ " - ~~~, ~k~!!. ~~~~'~f~~t\ ~, Department of International Center for Living ~che Gesellschaft fOrTechnische Fisheries, Thailand Aquatic Resources Management Zusammenarbeit (GTZ) GmbH Bivalve Mollusc Culture Research in Thailand Edited by E.W. McCoy '5 and An account of research conducted under the project: TECHNICAL ASSISTANCE FOR APPLIED RESEARCH ON COASTAL AQUACULTURE A cooperative Project of the Department of Fisheries, Royal Kingdom of Thailand; the International Center for Living Aquatic Resources Management (ICLARM); and the Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) GmbH DEPARTMENT OF FISHERIES BANGKOK, THAILAND INTERNATIONAL CENTER FOR LIVING AQUATIC RESOURCES MANAGEMENT MANILA, PHILIPPINES DEUTSCHE GESELLSCHAFT FUR TECHNISCHE ZUSAMMENARBEIT (GTZ) GmbH ESCHBORN, FEDERAL REPUBLIC OF GERMANY Bivalve mollusc culture research in Thailand Edited by E.W. McCoy TANITTHACHONGPEEPIEN Published jointly by the Department of Fisheries, Bangkhen, Bangkok 10900, Thailand; International Center for Living Aquatic Resources Management, MC P.O. Box 1501, Makati, Metro Manila, Philippines; and Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ), GmbH, Postfach 5180, D-6236 Eschborn 1 be; FrankfurVMain, Federal Republic of Germany. Printed in Manila, Philippines McCoy, E.W. and T. Chongpeepien, Editors. 1988. Bivalve mollusc culture research in Thailand. ICLARM Technical Reports 19, 170 p. Department of Fisheries, Bangkok, Thailand; International Center for Livirig Aquatic Resources Management, Manila, Philippines; and Deutsche Gesellschaft filr Technische Zusarnmenarbeit (GTZ), GmbH, Eschborn, Federal Republic of Germany. ISSN 01 15-5547 ISBN 971 -1022-43-5 Cover: Steaming green mussels, Petchaburi, Thailand Photo by Ronald Ventilla.