DOCSLIB.ORG

Analysis of Polyphenolic Content in Marine and Aquatic Angiosperms from Norwegian Coastal Waters

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Analysis of Polyphenolic Content in Marine and Aquatic Angiosperms from Norwegian Coastal Waters Analysis of polyphenolic content in marine and aquatic angiosperms from Norwegian coastal waters Kjersti Hasle Enerstvedi Thesis for the Degree of Philosophiae Doctor (PhD) University of Bergen, Norway 2018 Analysis of polyphenolic content in marine and aquatic angiosperms from Norwegian coastal waters Kjersti Hasle Enerstvedi ThesisAvhandling for the for Degree graden of philosophiaePhilosophiae doctorDoctor (ph.d (PhD). ) atved the Universitetet University of i BergenBergen 20182017 DateDato of fordefence: disputas: 26.04.2018 1111 © Copyright Kjersti Hasle Enerstvedi The material in this publication is covered by the provisions of the Copyright Act. Year: 2018 Title: Analysis of polyphenolic content in marine and aquatic angiosperms from Norwegian coastal waters Name: Kjersti Hasle Enerstvedi Print: Skipnes Kommunikasjon / University of Bergen ANALYSIS OF POLYPHENOLIC CONTENT IN MARINE AND AQUATIC ANGIOSPERMS FROM NORWEGIAN COASTAL WATERS Kjersti Hasle Enerstvedt Dissertation for the degree philosophiae doctor (PhD) Department of Chemistry University of Bergen 2018 © Kjersti Hasle Enerstvedt, 2018 Department of Chemistry, University of Bergen Allégt. 41, 5007 Bergen, Norway Table of contents Preface Acknowledgements Abstract Abbreviations List of publications 1. INTRODUCTION ...................................................................................................... 1 1.1 Flavonoids ............................................................................................................. 1 1.1.1 Flavonoid structure ........................................................................................ 1 1.1.2 Biosynthesis ................................................................................................... 4 1.1.3 Plant function ................................................................................................. 5 1.1.4 Biological activities ....................................................................................... 6 1.1.5 Sulphated flavonoids ...................................................................................... 8 1.2 Phenolic acids ....................................................................................................... 9 1.2.1 Structure ......................................................................................................... 9 1.2.2 Biosynthesis ................................................................................................... 9 1.2.3 Plant function and biological activity .......................................................... 10 1.3 Flavonoids and phenolic acids in marine angiosperms ...................................... 10 1.3.1 Seagrasses .................................................................................................... 10 1.3.2 Flavonoids in seagrasses .............................................................................. 11 1.3.3 Phenolic acids in seagrasses ........................................................................ 12 1.3.4 Quantitative measurements .......................................................................... 12 1.3.5 Seasonal fluctuation ..................................................................................... 13 2. METHODS ............................................................................................................... 21 2.1 Plant sampling ..................................................................................................... 21 2.2 Extraction and purification ................................................................................. 23 iii 2.2.1 Extraction ..................................................................................................... 23 2.2.2 Liquid-liquid partitioning ............................................................................ 23 2.2.3 Amberlite XAD-7 (adsorption chromatography) ........................................ 23 2.3 Separation and isolation ...................................................................................... 24 2.3.1 Sixe exclusion column chromatography ...................................................... 24 2.3.2 Preparative High Performance Liquid Chromatography (HPLC) ............... 24 2.4 Analytical methods ............................................................................................. 25 2.4.1 Analytical HPLC .......................................................................................... 25 2.4.2 Quantitative determinations ......................................................................... 26 2.4.3 Nuclear Magnetic Resonance (NMR) spectroscopy .................................... 27 2.4.4 Mass spectrometry (MS) .............................................................................. 30 2.4.5 Ultraviolet/Visible Spetroscopy (UV-Vis) .................................................. 30 2.5 Antioxidant activity ............................................................................................ 31 2.5.1 DPPH scavenging ........................................................................................ 31 3. RESULTS AND DISCUSSION ............................................................................... 35 3.1 Characterization of flavonoids in marine and aquatic angiosperms ................... 36 3.1.1 Characterization of flavonoids in Zostera (paper I and II) .......................... 36 3.1.2 Characterization of flavonoids in Ruppia (paper III) .................................. 40 3.2 Characteristics of sulphated flavonoids (paper I) ............................................... 44 3.2.1 UV-Vis spectroscopy ................................................................................... 44 3.2.2 NMR spectroscopy ...................................................................................... 44 3.2.3 Stability of sulphated flavonoids in extract and as purified compounds ..... 46 3.3. Quantitative content of flavonoids and phenolic acids in Zostera and Ruppia . 49 3.3.1 Regional differences Zostera (paper II) ....................................................... 50 3.3.2 Regional differences Ruppia (paper III) ...................................................... 52 3.4 Seasonal variation ............................................................................................... 54 3.4.1 Year-to-year variation in Z. marina (paper II) ............................................. 54 3.4.2. Seasonal variation in Zostera ...................................................................... 55 3.4.3 Seasonal variation in Ruppia species (paper II) .......................................... 57 iv 3.4.4 Comparison of flavonoid content and seasonal variation in Zostera and Ruppia . .................................................................................................................. 58 3.5 Antioxidant properties ........................................................................................ 61 3.5.1 DPPH radical scavenging capacity .............................................................. 61 REFERENCES ……………………………………………………………………… 65 APPENDIX A. An overview of the compounds involved in the thesis B. Structures of the compounds involved in the thesis C. 1H NMR data Table C-1. Compound 1, 3, 4, 6, 9, 12 , 14 and 15 Table C-2. Compound 16, 17, 19 and CA Table C-3. Compound 20 ‒23 D. 13 C NMR data Table D-1. Compound 1, 3, 4, 6, 9, 12 , 14 and 15 Table D-2. Compound 16, 17, 19 ‒23 and CA E. 1H NMR spectra Figure E-1‒E-11. Compound 1, 3, 4, 6, 9, 12 , 15 ‒17 , 19 ‒21 and CA F. 13 C and 2D NMR spectra Figure F-1‒F-11. Compound 1, 3, 4, 6, 9, 12 , 15 ‒17 , 19 ‒23 and CA G. Quantitative data Table G-1. Compounds in Z. marina collected from Espegrend, 2014 ‒2017 Table G-2. Compounds in Z. noltii collected from Huglo, 2016 ‒2017 H. Extraction optimization Figure H-1. Extraction yield vs. solvent concentration Figure H-1. Extraction yield vs. number of extractions PAPERS I-III v Preface This thesis is submitted for the degree of Philosophiae Doctor (PhD) in Chemistry at the University of Bergen, Norway. The experimental part of the work was carried out at the Department of Chemistry, University of Bergen, during the period 2013 ‒2017 under the supervision of Assoc. Professor Monica Jordheim and co-supervisor Professor Øyvind M. Andersen. The thesis consists of three papers ( I‒III ) preceded by an extended summary. Chapter 1 gives an introduction to the thesis, chapter 2 presents the methods used in this work, and chapter 3 covers the results from papers I‒III . The Appendix section includes an overview of the compounds in this work (A) and their structures (B), 1H and 13 C NMR data (C and D), as well as both 1D and 2D NMR spectra (E and F) for compounds 1, 3, 4, 6, 9, 12 , 15 ‒17 , 19 ‒23 and CA . Additional quantitative data for compounds 1‒ 12 and RA are found in Appendix G, and extraction optimization data are found in Appendix H. vi Acknowledgements First and foremost I would like to thank my supervisors Assoc. Professor Monica Jordheim and Professor Øyvind M. Andersen for excellent supervision. To my main supervisor, Monica Jordheim, I am truly grateful for the guidance and invaluable support you have offered during this work. I would also like to thank my co-authors, especially Professor Anders Lundberg for sharing your knowledge on marine plants, and for help with collection and identification of botanical material. I am grateful to The Research Council of Norway for financial support through the Norwegian NMR Platform,
Load more

Recommended publications
  • Introduction to Common Native & Invasive Freshwater Plants in Alaska
    Introduction to Common Native & Potential Invasive Freshwater Plants in Alaska Cover photographs by (top to bottom, left to right): Tara Chestnut/Hannah E. Anderson, Jamie Fenneman, Vanessa Morgan, Dana Visalli, Jamie Fenneman, Lynda K. Moore and Denny Lassuy. Introduction to Common Native & Potential Invasive Freshwater Plants in Alaska This document is based on An Aquatic Plant Identification Manual for Washington’s Freshwater Plants, which was modified with permission from the Washington State Department of Ecology, by the Center for Lakes and Reservoirs at Portland State University for Alaska Department of Fish and Game US Fish & Wildlife Service - Coastal Program US Fish & Wildlife Service - Aquatic Invasive Species Program December 2009 TABLE OF CONTENTS TABLE OF CONTENTS Acknowledgments ............................................................................ x Introduction Overview ............................................................................. xvi How to Use This Manual .................................................... xvi Categories of Special Interest Imperiled, Rare and Uncommon Aquatic Species ..................... xx Indigenous Peoples Use of Aquatic Plants .............................. xxi Invasive Aquatic Plants Impacts ................................................................................. xxi Vectors ................................................................................. xxii Prevention Tips .................................................... xxii Early Detection and Reporting
    [Show full text]
  • An Inventory of Rare Plants of Misty Fiords National Monument, Usda Forest Service, Region Ten
    AN INVENTORY OF RARE PLANTS OF MISTY FIORDS NATIONAL MONUMENT, USDA FOREST SERVICE, REGION TEN A Report by John DeLapp Alaska Natural Heritage Program ENVIRONMENT AND NATURAL RESOURCES INSTITUTE University of Alaska Anchorage 707 A Street, Anchorage, Alaska 99501 February 8, 1994 ALASKA NATURAL HERITAGE PROGRAM ENVIRONMENT AND NATURAL RESOURCES INSTITUTE UNIVERSITY OF ALASKA ANCHORAGE 707 A Street • Anchorage, Alaska 99501 • (907) 279-4523 • Fax (907) 276-6847 Dr. Douglas A. Segar, Director Dr. David C. Duffy, Program Manager (UAA IS AN EO/AA EMPLOYER AND EDUCATIONAL INSTITUTION) 2 ACKNOWLEDGEMENTS This cooperative project was the result of many hours of work by people within the Misty Fiords National Monument and the Ketchikan Area of the U.S. Forest Service who were dedicated to our common objectives and we are grateful to them all. Misty Fiords personnel who were key to the initiation and realization of this project include Jackie Canterbury and Don Fisher. Becky Nourse, Mark Jaqua, and Jan Peloskey all provided essential support during the field surveys. Also, Ketchikan Area staff Cole Crocker-Bedford, Michael Brown, and Richard Guhl provided indispensable support. Others outside of the Forest Service have provided assistance, without which this report would not be possible. Of particular note are Dr. David Murray, Dr. Barbara Murray, Carolyn Parker, and Al Batten of the University of Alaska Fairbanks Museum Herbarium. 3 TABLE OF CONTENTS ACKNOWLEDGEMENTS..........................................................................................................
    [Show full text]
  • Variation, Adaptation and Reproductive Biology In
    University of New Hampshire University of New Hampshire Scholars' Repository Doctoral Dissertations Student Scholarship Winter 1983 VARIATION, ADAPTATION AND REPRODUCTIVE BIOLOGY IN RUPPIA MARITIMA L POPULATIONS FROM NEW HAMPSHIRE COASTAL AND ESTUARINE TIDAL MARSHES FRANK AD VID RICHARDSON University of New Hampshire, Durham Follow this and additional works at: https://scholars.unh.edu/dissertation Recommended Citation RICHARDSON, FRANK DAVID, "VARIATION, ADAPTATION AND REPRODUCTIVE BIOLOGY IN RUPPIA MARITIMA L POPULATIONS FROM NEW HAMPSHIRE COASTAL AND ESTUARINE TIDAL MARSHES" (1983). Doctoral Dissertations. 1417. https://scholars.unh.edu/dissertation/1417 This Dissertation is brought to you for free and open access by the Student Scholarship at University of New Hampshire Scholars' Repository. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of University of New Hampshire Scholars' Repository. For more information, please contact [email protected]. INFORMATION TO USERS This reproduction was made from a copy of a document sent to us for microfilming. While the most advanced technology has been used to photograph and reproduce this document, the quality of the reproduction is heavily dependent upon the quality of the material submitted. The following explanation of techniques is provided to help clarify markings or notations which may appear on this reproduction. 1. The sign or “target” for pages apparently lacking from the document photographed is “Missing Page(s)”. If it was possible to obtain the missing page(s) or section, they are spliced into the film along with adjacent pages. This may have necessitated cutting through an image and duplicating adjacent pages to assure complete continuity. 2.
    [Show full text]
  • Checklist of Montana Vascular Plants
    Checklist of Montana Vascular Plants June 1, 2011 By Scott Mincemoyer Montana Natural Heritage Program Helena, MT This checklist of Montana vascular plants is organized by Division, Class and Family. Species are listed alphabetically within this hierarchy. Synonyms, if any, are listed below each species and are slightly indented from the main species list. The list is generally composed of species which have been documented in the state and are vouchered by a specimen collection deposited at a recognized herbaria. Additionally, some species are included on the list based on their presence in the state being reported in published and unpublished botanical literature or through data submitted to MTNHP. The checklist is made possible by the contributions of numerous botanists, natural resource professionals and plant enthusiasts throughout Montana’s history. Recent work by Peter Lesica on a revised Flora of Montana (Lesica 2011) has been invaluable for compiling this checklist as has Lavin and Seibert’s “Grasses of Montana” (2011). Additionally, published volumes of the Flora of North America (FNA 1993+) have also proved very beneficial during this process. The taxonomy and nomenclature used in this checklist relies heavily on these previously mentioned resources, but does not strictly follow anyone of them. The Checklist of Montana Vascular Plants can be viewed or downloaded from the Montana Natural Heritage Program’s website at: http://mtnhp.org/plants/default.asp This publication will be updated periodically with more frequent revisions anticipated initially due to the need for further review of the taxonomy and nomenclature of particular taxonomic groups (e.g. Arabis s.l ., Crataegus , Physaria ) and the need to clarify the presence or absence in the state of some species.
    [Show full text]
  • Lista Rossa Vol.2 Flora Italiana
    REALIZZATO DA LISTA ROSSA DELLA FLORA ITALIANA 2. ENDEMITI e altre specie minacciate WWW.IUCN.ITWWW.IUCN.IT 1 LISTA ROSSA della flora italiana 2. ENDEMITI e altre specie minacciate 2 Lista Rossa IUCN della flora italiana:2. ENDEMITI e altre piante minacciate Pubblicazione realizzata nell’ambito dell’accordo quadro “Per una più organica collaborazione in tema di conservazione della biodiversità”, sottoscritto da Ministero dell’Ambiente e della Tutela del Territorio e del Mare e Federazione Italiana Parchi e Riserve Naturali. Compilata da Graziano Rossi, Simone Orsenigo, Domenico Gargano, Chiara Montagnani, Lorenzo Peruzzi, Giuseppe Fenu, Thomas Abeli, Alessandro Alessandrini, Giovanni Astuti, Gian- luigi Bacchetta, Fabrizio Bartolucci, Liliana Bernardo, Maurizio Bovio, Salvatore Brullo, Angelino Carta, Miris Castello, Fabio Conti, Donatella Cogoni, Gianniantonio Domina, Bruno Foggi, Matilde Gennai, Daniela Gigante, Mauro Iberite, Cesare Lasen, Sara Ma- grini, Gianluca Nicolella, Maria Silvia Pinna, Laura Poggio, Filippo Prosser, Annalisa Santangelo, Alberto Selvaggi, Adriano Stinca, Nicoletta Tartaglini, Angelo Troia, Maria Cristina Villani, Robert Wagensommer, Thomas Wilhalm, Carlo Blasi. Citazione consigliata Rossi G., Orsenigo S., Gargano D., Montagnani C., Peruzzi L., Fenu G., Abeli T., Alessan- drini A., Astuti G., Bacchetta G., Bartolucci F., Bernardo L., Bovio M., Brullo S., Carta A., Castello M., Cogoni D., Conti F., Domina G., Foggi B., Gennai M., Gigante D., Iberite M., Lasen C., Magrini S., Nicolella G., Pinna M.S., Poggio L., Prosser F., Santangelo A., Selvaggi A., Stinca A., Tartaglini N., Troia A., Villani M.C., Wagensommer R.P., Wilhalm T., Blasi C., 2020. Lista Rossa della Flora Italiana. 2 Endemiti e altre specie minacciate. Ministero dell’Ambiente e della Tutela del Territorio e del Mare Foto in copertina Astragalus gennarii, Gravemente Minacciata (CR), Foto © G.
    [Show full text]
  • Potamogetonaceae, Zosteraceae
    Flora Malesiana, Series I, Volume 16 (2002) 167-216 Potamogetonaceae, Zosteraceae, and Cymodoceaceae 1 C. den Hartog Nijmegen, The Netherlands & G. Wiegleb Cottbus, Germany) Introductionto thesea-grasses (C. den Hartog) In earlier papers the sea-grasses were classifiedwithin two families, the Potamogetona- and the As result of research of all of the ceae Hydrocharitaceae. a thorough genera order Helobiae (Alismatidae) by Tomlinson (1982), it has become evident that the very heterogeneous family Potamogetonaceae had to be split into a number of independent families. The sea-grasses which already had subfamily status, became families in their own right, the Cymodoceaceae the Zosteraceae and the Posidoniaceae (not in Malesia). , , The independence of these families is not contradicted by molecular genetical evidence (Les et al. 1997). According to the new vision the Potamogetonaceae are restricted to the genera Potamogeton, Groenlandia (not in Malesia), and Ruppia; however, there are of its molecular genetic indications that the latter genus may present a family own (Les et al. 1997). Therefore, and because of the comparable role in the vegetation of the dif- ferent introduction to in is here, with to the genera, an sea-grasses general given a Key different families and genera of sea-grasses. The phytochemistry of all these groups is given by R. Hegnauer. The few angiosperms that have penetrated into the marine environment, and are able to fulfiltheir vegetative and generative cycle when completely submerged, are generally known as sea-grasses. The name refers to the superficial resemblance to grasses, be- cause ofthe linear leaves of most of the species. In spite of the fact that the numberof sea-grass species is very small (only 60 to 65), they are of paramount importance in the coastal environment, where, when they occur, they generally form extensive beds.
    [Show full text]
  • Yukon Conservation Data Centre's
    Yukon Conservation Data Centre Vascular plant track list Updated February 2019 This is a list of vascular plants that are considered of conservation concern in Yukon by the Yukon Conservation Data Centre. We actively track information on these plant species and map all known locations in our database. We encourage all to report sightings of these species to us with as much detail as possible. Field observation forms (available for download on our website) can assist with this reporting. Documentation, such as a specimen or a photograph, is required to confirm identification. Scientific Name Common Name G Rank* N Rank* S Rank* Synonyms Alisma triviale Northern Water-plantain G5 N5 S1 Alisma plantago-aquatica var. americanum Gnaphalium margaritaceum, Anaphalis Anaphalis margaritacea Pearly Everlasting G5 N5 S2 margaritacea var. subalpina Cynoglossum boreale, Cynoglossum Andersonglossum boreale Northern Wild Comfrey G5T4T5 N4N5 S1 virginianum var. boreale Braya eschscholtziana, Eutrema Aphragmus eschscholtzianus Aleutian Cress G4? N3 S2S3 eschscholtzianum Arabis media, Arabidopsis petraea ssp. Arabidopsis lyrata ssp. petraea Sand-dune Rockcress GNR N1N2 S1S2 umbrosa Armeria maritima ssp. sibirica Arctic Thrift G5T5 N4N5 S3 Armeria maritima ssp. arctica Arnica parryi ssp. parryi, Arnica parryi ssp. Arnica parryi Parry's Arnica G5 N5 S1 genuina Artemisia globularia Purple Wormwood G4 N2N3 S2S3 Artemisia laciniata Siberian Wormwood GNR N3 S3 Artemisia tanacetifolia Artemisia woodii Yukon Wormwood G3?T2T3 N2N3 S2S3 Artemisia rupestris ssp. woodii Aruncus dioicus var. acuminatus Common Goat's-beard G5T5 N5 SH Aruncus acuminatus, Spiraea acuminata Asplenium viride Green Spleenwort G5 N5 S1 Asplenium trichomanes-ramosum Athyrium distentifolium ssp. americanum, Athyrium alpestre ssp. Athyrium distentifolium var.
    [Show full text]
  • Database and Atlas of Aquatic Vascular Plants the British Isles
    f t 3 DATABASE AND ATLAS OF AQUATIC VASCULAR PLANTS THE BRITISH ISLES Part I : Species Accounts ITE NRA National Rivers Authority Project Record 352/2/N&Y ' NRA 352/2/N&Y fG 'S-C NATIONAL RIVERSAUTHCJRITY Database ami-*rtflas o-f a q u a tlp -^ 7 a s c u 1 ar p la n ts i j A JXC -tfT 1 so . 00 Database and Atlas of Aquatic Vascular Plants in the British Isles Part I: Species Accounts C D Preston and J M Croft Research Contractor: Institute of Freshwater Ecology Monks Wood Abbots Ripton Huntingdon Cambridge PE17 2LS National Rivers Authority Rivers House Waterside Drive Almondsbury Bristol BS12 4UD Project Record 352/2/N&Y ENVIRONMENT AGENCY 136210 Commissioning Organisation National Rivers Authority Rivers House Waterside Drive Almondsbury . Bristol BS12 4UD Tel: 01454 624400 Fax: 01454 624409 ® National Rivers Authority 1995 . All rights reserved. No part of this document may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise without the prior permission of the National Rivers Authority. The views expressed in this document are not necessarily those of the NRA. Its officers, servants or agents accept no liability for any loss or damage arising from the interpretation or use of the information, or reliance upon views contained herein. Dissemination Status Internal: Limited Release External: Restricted Statement of Use This document provides information on the occurrence and distribution of aquatic plants in Britain and provides a valuable source of data fro NRA staff.
    [Show full text]
  • Irish Botanical News No 31, 2021
    Irish Botanical News No. 31 March 2021 Editors: Paul R. Green & Alexis FitzGerald Neotinea maculata (Dense-flowered Orchid) at Knocknarea, Co. Sligo. Photo E. Gaughan © 2020 (p. 82) Contributions intended for Irish Botanical News No. 32 Should reach the Editor Alexis FitzGerald before January 31st 2022 E-mail: [email protected] Coliemore Apts., Coliemore Road, Dalkey, Co. Dublin, A96 E086 PAGE 1 Committee for Ireland 2020 –2021 The following is the Committee as elected at the Annual General Meeting via Zoom on 26th September 2020. Office bearers were subsequently elected at the first committee meeting. The Committee is now: Edwina Cole (Chair, Irish Officer Steering Group) Ralph Sheppard (Vice-Chair) Vacant (Secretary) Mark McCorry (Field Secretary) Rory Hodd (Hon. Treasurer) Shane Brien Cliona Byrne John Faulkner (Board of Trustees) Alexis FitzGerald Jessica Hamilton David McNeill Robert Northridge (Ireland Officer Steering Group) The following are nominated observers to the committee: Abigail Maiden (Northern Ireland Environment Agency) Mike Wyse Jackson (National Parks & Wildlife Service) Draft Minutes of the BSBI Irish Branch AGM 2020 are available at: http://governance.bsbi.org/ireland Irish Botanical News is published by the committee for Ireland, BSBI and edited by P.R. Green and A. FitzGerald. © P.R. Green, A. FitzGerald and the authors of individual articles, 2021. Any opinions expressed in the articles below are those of the authors and do not necessarily reflect the views of BSBI. Front cover photo: Elsie Reynolds, age 6 ½, examining a Dandelion during a FaceTime session with her grandmother Sylvia Reynolds. Photo Owen Reynolds © 2020 (p. 82). All species and common names in Irish Botanical News follow those in the database on the BSBI website http://rbg-web2.rbge.org.uk/BSBI/ and Stace, C.
    [Show full text]
  • Flora Acuática Española. Hidrófitos Vasculares
    Flora acuática Española Hidrófitos vasculares Flora acuática española Hidrófitos vasculares Flora acuática española Hidrófitos vasculares Santos Cirujano Bracamonte Ana Meco Molina Pablo García Murillo Ilustraciones Marta Chirino Argenta Madrid, 2014 CIRUJANO BRACAMONTE, S., MECO MOLINA, A., GARCÍA MURILLO, P. & CHIRINO ARGENTA, M. 2014. Flora acuática española. Hidrófitos vasculares. Real Jardín Botánico, CSIC, Madrid. Fotografías: Agradecemos a los siguientes autores la cesión altruista de sus fotografías. En dominio público: Fig. 158 (http://fish.kiev.ua); Fig. 172 (MARTIN CHYTRY); Vallisneria spiralis (http://fish.kiev.ua); Fig. 287 [TIM CARRUTHERS (ian.umces.edu/imagelibrary)]. Con licencia Creative Commons (http://creativecommons.org/licenses/): Fig. 129 (ANDRÉ KARWATH AKA, CC BY-SA); Fig. 142 (JÖRG HEMPEL, CC BY-SA); Fig. 152 (VITAL SIGN USER ¡SPYASIGN, CC BY); Fig. 186 (H. ZELL, CC BY-SA); Fig.199 (ANDEA MORO, CC NC-BY-BA); Fig. 207 (BOOLON, CC BY); Fig. 219 (© Garcete-Barret); Fig. 222 (GERRIT DAVIDSE, CC BY-NC-SA); Fig. 228 (DAVID PÉREZ, CC BY); Fig. 235 (CHRISTIAN FISCHER, CC BY-SA); Fig. 237 (A. A. BOBROV, CC BY); Fig. 245 (CHRISTIAN FISCHER, CC BY-SA); Fig. 262 (A. A. BOBROV, CC BY); Fig. 282 (BERND H., CC BY-SA). Con copyright: Fig. 92 (© JOSÉ QUILES); Fig. 148 (© JIRÍ KAMENÍCEK); Fig. 171 (© RUSS KLEINMAN); Fig. 184 (© CHRIS MOODY); Fig. 221 (© JULIANO ALVES FEITOSA); Fig. 223 (© MANU SANFÉLIX); Fig. 247 (© JAN SEVCIK); Fig. 269 [© DAVID FENWICK (www.aphtoflora.com)]; Fig. 296 (© JIRÍ KAMENÍCEK); Fig. 310 [© JÉRÔME PICARD (CHALLET-HÉRAULT)]; Fig. 305 (© JIRÍ KAMENÍCEK); Fig. 326 (© CHRIS PICKERELL); Fig. 337 (© Pierre Danet). Otros autores: Figs. 168, 313 (PERE FRAGA I ARGUIMBAU); Fig.
    [Show full text]
  • Review on the Conservation Status of Autochthonous Marine Angiosperms in the Mediterranean Sea
    Review on the conservation status of autochthonous marine angiosperms in the Mediterranean Sea Alumno: Alice Carrara Tutor: Jorge Juan Vicedo Curso académico: 2019/2020 Alice Carrara Alice Carrara TABLE OF CONTENTS List of figures .................................................................................................................... IV List of tables ...................................................................................................................... VI Abstract ............................................................................................................................. 1 Resumen ............................................................................................................................ 2 1. INTRODUCTION .............................................................................................................. 3 1.1 Seagrasses ............................................................................................................................. 3 1.1.1 Biology and diversity of seagrasses ....................................................................................................... 3 1.1.2 Global distribution ................................................................................................................................. 7 1.1.3 Main threats .......................................................................................................................................... 9 1.2 Plant conservation strategies ..............................................................................................
    [Show full text]
  • Volume 106 2021 Annals of the Missouri Botanical Garden
    Volume 106 Annals 2021 of the Missouri Botanical Garden A CONTRIBUTION TO THE Olga De Castro,2 Anna Geraci,3 Anna Maria CHARACTERIZATION OF RUPPIA Mannino,3 Nicolina Mormile,2 Annalisa 2 3* DREPANENSIS (RUPPIACEAE), A Santangelo, and Angelo Troia KEY SPECIES OF THREATENED MEDITERRANEAN WETLANDS1 ABSTRACT To elucidate the taxonomic status of Ruppia drepanensis Tineo ex Guss. (Alismatales, Ruppiaceae), we performed morpho- logical analysis and DNA barcoding of historical materials (including the lectotype) and fresh samples (including those from a recently discovered population near the locus classicus in Sicily, Italy). We conclude that R. drepanensis is a separate species, closely related to R. spiralis L. ex Dumort., that occurs in temporary inland waters from the western to central sectors of the Mediterranean region. We also highlight the importance of vouchers and the need to link molecular investigations to field, ecological, and morphological investigations. Key words: Aquatic meadows, DNA barcoding, herbarium, historical specimens, ITS, morphology, Ruppia, Ruppiaceae, seagrass, typification. Mediterranean wetlands are under severe human Troia & Lansdown, 2016; Guarino et al., 2019), we pressure (Fraixedas et al., 2019; Geijzendorffer et al., found a population of Ruppia drepanensis Tineo ex 2019); in the framework of our studies on these threat- Guss. in Sicily (Italy), where it has not been recently ened habitats and their flora (Mannino & Geraci, 2016; reported. 1 We thank the herbaria NAP and PAL for permission to collect samples from historical specimens; the manager of the “Riserva Naturale Saline di Trapani e Paceco” for permission to collect samples from Salina Culcasi; Nobuyuki Tanaka, senior curator of TNS, for high-resolution images of some herbarium specimens; and Vincenza Polizzano for help with arranging and drying the fresh material.
    [Show full text]