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Plk1 Polyclonal Antibody Catalog # AP73237

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Plk1 Polyclonal Antibody Catalog # AP73237 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Plk1 Polyclonal Antibody Catalog # AP73237 Specification Plk1 Polyclonal Antibody - Product Information Application WB Primary Accession P53350 Reactivity Human, Mouse, Rat Host Rabbit Clonality Polyclonal Plk1 Polyclonal Antibody - Additional Information Gene ID 5347 Other Names PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13 Dilution WB~~Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other Plk1 Polyclonal Antibody - Background applications. Serine/threonine-protein kinase that performs Format several important functions throughout M Liquid in PBS containing 50% glycerol, 0.5% phase of the cell cycle, including the regulation BSA and 0.02% sodium azide. of centrosome maturation and spindle assembly, the removal of cohesins from Storage Conditions chromosome arms, the inactivation of -20℃ anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins Plk1 Polyclonal Antibody - Protein Information acts by binding and phosphorylating proteins are that already phosphorylated on a specific Name PLK1 motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, Synonyms PLK CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, Function NPM1, NUDC, PKMYT1/MYT1, KIZ, Serine/threonine-protein kinase that PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, performs several important functions SGO1, STAG2/SA2, TEX14, TOPORS, p73/TP73, throughout M phase of the cell cycle, TPT1, WEE1 and HNRNPU. Plays a key role in including the regulation of centrosome centrosome functions and the assembly of maturation and spindle assembly, the bipolar spindles by phosphorylating KIZ, removal of cohesins from chromosome NEDD1 and NINL. NEDD1 phosphorylation arms, the inactivation of anaphase- promotes subsequent targeting of the promoting complex/cyclosome (APC/C) gamma-tubulin ring complex (gTuRC) to the inhibitors, and the regulation of mitotic exit centrosome, an important step for spindle Page 1/6 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 and cytokinesis. Polo-like kinase proteins formation. Phosphorylation of NINL component acts by binding and phosphorylating of the centrosome leads to NINL dissociation proteins are that already phosphorylated on from other centrosomal proteins. Involved in a specific motif recognized by the POLO box mitosis exit and cytokinesis by phosphorylating domains. Phosphorylates BORA, CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1 and BUB1B/BUBR1, CCNB1, CDC25C, CEP55, RACGAP1. Recruited at the central spindle by ECT2, ERCC6L, FBXO5/EMI1, FOXM1, phosphorylating and docking PRC1 and KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, KIF20A/MKLP2; creates its own docking sites on NUDC, PKMYT1/MYT1, KIZ, PRC1 and KIF20A/MKLP2 by mediating PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, phosphorylation of sites subsequently SGO1, STAG2/SA2, TEX14, TOPORS, recognized by the POLO box domains. p73/TP73, TPT1, WEE1 and HNRNPU. Plays Phosphorylates RACGAP1, thereby creating a a key role in centrosome functions and the docking site for the Rho GTP exchange factor assembly of bipolar spindles by ECT2 that is essential for the cleavage furrow phosphorylating KIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes formation. Promotes the central spindle subsequent targeting of the gamma-tubulin recruitment of ECT2. Plays a central role in ring complex (gTuRC) to the centrosome, G2/M transition of mitotic cell cycle by an important step for spindle formation. phosphorylating CCNB1, CDC25C, FOXM1, Phosphorylation of NINL component of the CENPU, PKMYT1/MYT1, PPP1R12A/MYPT1 and centrosome leads to NINL dissociation from WEE1. Part of a regulatory circuit that other centrosomal proteins. Involved in promotes the activation of CDK1 by mitosis exit and cytokinesis by phosphorylating the positive regulator CDC25C phosphorylating CEP55, ECT2, and inhibiting the negative regulators WEE1 KIF20A/MKLP2, CENPU, PRC1 and RACGAP1. and PKMYT1/MYT1. Also acts by mediating Recruited at the central spindle by phosphorylation of cyclin-B1 (CCNB1) on phosphorylating and docking PRC1 and centrosomes in prophase. Phosphorylates KIF20A/MKLP2; creates its own docking sites FOXM1, a key mitotic transcription regulator, on PRC1 and KIF20A/MKLP2 by mediating leading to enhance FOXM1 transcriptional phosphorylation of sites subsequently activity. Involved in kinetochore functions and recognized by the POLO box domains. sister chromatid cohesion by phosphorylating Phosphorylates RACGAP1, thereby creating BUB1B/BUBR1, FBXO5/EMI1 and STAG2/SA2. a docking site for the Rho GTP exchange PLK1 is high on non-attached kinetochores factor ECT2 that is essential for the suggesting a role of PLK1 in kinetochore cleavage furrow formation. Promotes the attachment or in spindle assembly checkpoint central spindle recruitment of ECT2. Plays a (SAC) regulation. Required for kinetochore central role in G2/M transition of mitotic cell localization of BUB1B. Regulates the cycle by phosphorylating CCNB1, CDC25C, dissociation of cohesin from chromosomes by FOXM1, CENPU, PKMYT1/MYT1, phosphorylating cohesin subunits such as PPP1R12A/MYPT1 and WEE1. Part of a regulatory circuit that promotes the STAG2/SA2. Phosphorylates SGO1: required for activation of CDK1 by phosphorylating the spindle pole localization of isoform 3 of SGO1 positive regulator CDC25C and inhibiting and plays a role in regulating its centriole the negative regulators WEE1 and cohesion function. Mediates phosphorylation of PKMYT1/MYT1. Also acts by mediating FBXO5/EMI1, a negative regulator of the APC/C phosphorylation of cyclin-B1 (CCNB1) on complex during prophase, leading to centrosomes in prophase. Phosphorylates FBXO5/EMI1 ubiquitination and degradation by FOXM1, a key mitotic transcription the proteasome. Acts as a negative regulator regulator, leading to enhance FOXM1 of p53 family members: phosphorylates transcriptional activity. Involved in TOPORS, leading to inhibit the sumoylation of kinetochore functions and sister chromatid p53/TP53 and simultaneously enhance the cohesion by phosphorylating BUB1B/BUBR1, ubiquitination and subsequent degradation of FBXO5/EMI1 and STAG2/SA2. PLK1 is high p53/TP53. Phosphorylates the transactivation on non-attached kinetochores suggesting a domain of the transcription factor p73/TP73, role of PLK1 in kinetochore attachment or in leading to inhibit p73/TP73-mediated spindle assembly checkpoint (SAC) transcriptional activation and pro-apoptotic regulation. Required for kinetochore functions. Phosphorylates BORA, and thereby Page 2/6 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 localization of BUB1B. Regulates the promotes the degradation of BORA. dissociation of cohesin from chromosomes Contributes to the regulation of AURKA by phosphorylating cohesin subunits such function. Also required for recovery after DNA as STAG2/SA2. Phosphorylates SGO1: damage checkpoint and entry into mitosis. required for spindle pole localization of Phosphorylates MISP, leading to stabilization of isoform 3 of SGO1 and plays a role in cortical and astral microtubule attachments regulating its centriole cohesion function. required for proper spindle positioning Mediates phosphorylation of FBXO5/EMI1, a (PubMed:8991084, PubMed:11202906, negative regulator of the APC/C complex PubMed:12207013, PubMed:12447691, during prophase, leading to FBXO5/EMI1 PubMed:12524548, PubMed:12738781, ubiquitination and degradation by the PubMed:12852856, PubMed:12939256, proteasome. Acts as a negative regulator of PubMed:14532005, PubMed:14734534, p53 family members: phosphorylates PubMed:15070733, PubMed:15148369, TOPORS, leading to inhibit the sumoylation PubMed:15469984, PubMed:16198290, of p53/TP53 and simultaneously enhance the ubiquitination and subsequent PubMed:16247472, PubMed:16980960, degradation of p53/TP53. Phosphorylates PubMed:17081991, PubMed:17351640, the transactivation domain of the PubMed:17376779, PubMed:17617734, transcription factor p73/TP73, leading to PubMed:18174154, PubMed:18331714, inhibit p73/TP73-mediated transcriptional PubMed:18418051, PubMed:18477460, activation and pro-apoptotic functions. PubMed:18521620, PubMed:18615013, Phosphorylates BORA, and thereby PubMed:19160488, PubMed:19351716, promotes the degradation of BORA. PubMed:19468300, PubMed:19468302, Contributes to the regulation of AURKA PubMed:19473992, PubMed:19509060, function. Also required for recovery after PubMed:19597481, PubMed:23455478, DNA damage checkpoint and entry into PubMed:23509069). Together with MEIKIN, mitosis. Phosphorylates MISP, leading to acts as a regulator of kinetochore function stabilization of cortical and astral during meiosis I: required both for microtubule attachments required for mono-orientation of kinetochores on sister proper spindle positioning (PubMed:<a href chromosomes and protection of centromeric ="http://www.uniprot.org/citations/8991084 cohesin from separase-mediated cleavage (By " target="_blank">8991084</a>, similarity). Phosphorylates CEP68 and is PubMed:<a href="http://www.uniprot.org/ci required for its degradation tations/11202906" (PubMed:25503564). Regulates nuclear target="_blank">11202906</a>, envelope breakdown during prophase by PubMed:<a href="http://www.uniprot.org/ci phosphorylating DCTN1 resulting in its tations/12207013" localization in
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