Labstandard® PESTICIDE KIT SIMULTANEOUS •••••••••••• •••••••••••• 474 COMPONENTS for ANALYSIS of RESIDUAL “READY to USE” PESTICIDES USING GC- MS/MS

LabStandard® PESTICIDE KIT SIMULTANEOUS •••••••••••• •••••••••••• 474 COMPONENTS for ANALYSIS OF RESIDUAL “READY TO USE” PESTICIDES USING GC- MS/MS

The following LabStandard® Kit contains the pesticides mentioned by Agilent Technologies to realize and publish the Application Note “Simultaneous Analysis of 477 Residual Pesticides in Agricultural Crops Using GC-MS/MS” for determination of pesticides residues using GCMSMS TQ8040.

Code Concentration Solvent Packaging

KIT4BB3L474 100,00 mg/l Acetonitrile/ Methanol / Toluene 1,1 ml amber silanized ampoules

Shimadzu TQ8040 and Shimadzu are trademarks of Shimadzu Corporation

Analytes CAS Analytes CAS Analytes CAS Analytes CAS

1,4-Dichlorobenzene 106-46-7 Anilazine 101-05-3 Bifenox 42576-02-3 Cadusafos 95465-99-9

2,4,5-T-2-ethylhexyl 1928-47-8 Anilofos 64249-01-0 Bifenthrin 82657-04-3 Cafenstrole 125306-83-4

2,4,5-T-methyl 1928-37-6 Anthraquinone 84-65-1 Bioresmethrin 28434-01-7 Captafol 2425-06-1

2,4-D-butyl 94-80-4 Atraton 1610-17-9 Biphenyl 92-52-4 Captan 133-06-2

Acephate 30560-19-1 Atrazine 1912-24-9 Bitertanol 55179-31-2 Carbaryl 63-25-2

Acetamiprid 135410-20-7 Atrazine, Desethyl- 6190-65-4 Boscalid 188425-85-6 Carbetamide 16118-49-3

Acetochlor 34256-82-1 Azaconazole 60207-31-0 Bromacil 314-40-9 Carbofuran 1563-66-2

Acibenzolar-S-Methyl 135158-54-2 Azamethiphos 35575-96-3 Bromobutide 74712-19-9 Carbophenothion 786-19-6

Aclonifen 74070-46-5 Azinphos-ethyl 2642-71-9 Bromocyclen 1715-40-8 Carbosulfan 55285-14-8

Acrinathrin 101007-06-1 Azinphos-Methyl 86-50-0 Bromophos-Ethyl 4824-78-6 Carboxin 5234-68-4

Alachlor 15972-60-8 Azoxystrobin 131860-33-8 Bromophos-Methyl 2104-96-3 Carfentrazone-Ethyl 128638-02-1

Alanycarb 83130-01-2 Beflubutamid 113614-08-7 Bromopropylate 18181-80-1 Chinomethionat 2439-01-2

Aldicarb 116-06-3 Benalaxyl 71626-11-4 Bromuconazole 116255-48-2 Chlomethoxyfen 32861-85-1

Aldicarb-Sulfone 1646-88-4 Bendiocarb 22781-23-3 Bupirimate 41483-43-6 Chlorantraniliprole 500008-45-7

Aldrin 309-00-2 Benfluralin 1861-40-1 Buprofezin 69327-76-0 Chlorbenside 103-17-3

Allethrin 584-79-2 Benfuracarb 82560-54-1 Butachlor 23184-66-9 Chlorbufam 1967-16-4

Allidochlor 93-71-0 Benfuresate 68505-69-1 Butafenacil 134605-64-4 Chlordane, trans- 5103-74-2

Ametryn 834-12-8 Benoxacor 98730-04-2 Butamifos 36335-67-8 Chlorethoxyfos 54593-83-8

Aminocarb 2032-59-9 Benzoylprop-Ethyl 22212-55-1 Butralin 33629-47-9 Chlorfenapyr 122453-73-0

Amitraz 33089-61-1 Bifenazate 149877-41-8 Butylate 2008-41-5 Chlorfenson 80-33-1

Lab.Instruments s.r.l. Accreditations and Certifications 70013 Castellana Grotte(BA) Italy - S.S. 172 (Putignano-Alberobello) Km 28+200 ISO Guide 34:2009 Tel +39 080 4969746-9 - Fax +39 080 2121749 Email: [email protected] - Website: www.labinstruments.org ISO/IEC 17025:2005 Analytes CAS Analytes CAS Analytes CAS Analytes CAS

Chlorfenvinphos 470-90-6 DDE, p,p- 72-55-9 Endosulfansulfate 1031-07-8 Fluazinam 79622-59-6

Chloridazon 1698-60-8 DDT, o,p- 789-02-6 Endrin 72-20-8 Flubenzimine 37893-02-0

Chlormephos 24934-91-6 DDT, p,p- 50-29-3 EPN 2104-64-5 Flucythrinate 70124-77-5

Chlornitrofen 1836-77-7 Deltamethrin 52918-63-5 Epoxiconazole 133855-98-8 Fludioxonil 131341-86-1

Chlorobenzilate 510-15-6 Demeton-S-Methyl 919-86-8 EPTC 759-94-4 Flufenacet 142459-58-3

Chloroneb 2675-77-6 Demeton-S-Methylsulfone 17040-19-6 Esfenvalerate 66230-04-4 Flufenpyr-ethyl 188489-07-8

Chloropropylate 5836-10-2 Desmedipham 13684-56-5 Esprocarb 85785-20-2 Flumiclorac-pentyl 87546-18-7

Chlorothalonil 1897-45-6 Dialifos 10311-84-9 Etaconazole 60207-93-4 Flumioxazin 103361-09-7

Chlorpropham 101-21-3 Di-Allate 2303-16-4 Ethalfluralin 55283-68-6 Fluopicolide 239110-15-7

Chlorpyrifos-Ethyl 2921-88-2 Diazinon 333-41-5 Ethiofencarb 29973-13-5 Fluotrimazole 31251-03-3

Chlorpyrifos-Methyl 5598-13-0 Dichlobenil 1194-65-6 Ethion 563-12-2 Fluquinconazole 136426-54-5

Chlorthal-Dimethyl 1861-32-1 Dichlofenthion 97-17-6 Ethofumesate 26225-79-6 Fluridone 59756-60-4

Chlorthiamid 1918-13-4 Dichlofluanid 1085-98-9 Ethoprophos 13194-48-4 Flusilazole 85509-19-9

Chlorthion 500-28-7 Dichlorvos 62-73-7 Ethychlozate 27512-72-7 Fluthiacet-methyl 117337-19-6

Chlorthiophos 60238-56-4 Diclobutrazol 75736-33-3 Etobenzanid 79540-50-4 Flutolanil 66332-96-5

Chlozolinate 84332-86-5 Diclocymet 139920-32-4 Etofenprox 80844-07-1 Flutriafol 76674-21-0

Cinidon-Ethyl 142891-20-1 Diclofop-Methyl 51338-27-3 Etoxazole 153233-91-1 Fluvalinate 69409-94-5

Cinmethylin 87818-31-3 Dicloran 99-30-9 Etridiazole 2593-15-9 Fluvalinate, tau- 102851-06-9 cis-Chlordene 56534-02-2 Dicofol 115-32-2 Etrimfos 38260-54-7 Folpet 133-07-3

Clodinafop-Propargyl 105512-06-9 Dicrotophos 141-66-2 Famoxadone 131807-57-3 Fonofos 944-22-9

Clofentezine 74115-24-5 Dieldrin 60-57-1 Famphur 52-85-7 Formothion 2540-82-1

Clomazone 81777-89-1 Diethofencarb 87130-20-9 Fenamidone 161326-34-7 Fosthiazate 98886-44-3

Clomeprop 84496-56-0 Difenoconazole 119446-68-3 Fenamiphos 22224-92-6 Furalaxyl 57646-30-7

Clothianidin 210880-92-5 Diflufenican 83164-33-4 Fenarimol 60168-88-9 Furametpyr 123572-88-3

Coumaphos 56-72-4 Dimepiperate 61432-55-1 Fenazaquin 120928-09-8 Furametpyr-hydroxy

Crimidine 535-89-7 Dimethachlor 50563-36-5 Fenbuconazole 114369-43-6 Furathiocarb 65907-30-4

Cyanazine 21725-46-2 Dimethametryn 22936-75-0 Fenchlorphos 299-84-3 Furilazole 121776-33-8

Cyanofenphos 13067-93-1 Dimethenamid 87674-68-8 Fenitrothion 122-14-5 Halfenprox 111872-58-3

Cyanophos 2636-26-2 Dimethipin 55290-64-7 Fenothiocarb 62850-32-2 HCH, alpha- 319-84-6

Cyantraniliprole 736994-63-1 Dimethoate 60-51-5 Fenoxanil 115852-48-7 HCH, beta- 319-85-7

Cycloate 1134-23-2 Dimethomorph 110488-70-5 Fenoxaprop-ethyl 66441-23-4 HCH, delta- 319-86-8

Cyflufenamid 180409-60-3 Dimethylvinphos 2274-67-1 Fenoxycarb 72490-01-8 HCH, gamma- 58-89-9

Cyfluthrin, beta- 68359-37-5 Diniconazole 83657-24-3 Fenpropathrin 39515-41-8 Heptachlor 76-44-8

Cyhalofop-Butyl 122008-85-9 Diofenolan 63837-33-2 Fenpropidin 67306-00-7 Heptachlorepoxid, cis- 1024-57-3

Cyhalothrin 68085-85-8 Dioxabenzofos 3811-49-2 Fenpropimorph 67564-91-4 Heptachlorepoxid, trans- 28044-83-9

Cyhalothrin, lambda- 91465-08-6 Dioxathion 78-34-2 Fenson 80-38-6 Heptenophos 23560-59-0

Cymiazole 61676-87-7 Diphenamid 957-51-7 Fensulfothion 115-90-2 Hexachlorobenzene 118-74-1

Cypermethrin 52315-07-8 Diphenylamine 122-39-4 Fenthion 55-38-9 Hexaconazole 79983-71-4

Cyproconazole 94361-06-5 Disulfoton 298-04-4 Fenvalerate 51630-58-1 Hexazinone 51235-04-2

Cyprodinil 121552-61-2 Disulfoton-Sulfone 2497-06-5 Ferimzone 89269-64-7 Hymexazol 10004-44-1

Cyprofuram 69581-33-5 Ditalimfos 5131-24-8 Fipronil 120068-37-3 Imazalil 35554-44-0

DCIP 620-45-1 Dithiopyr 97886-45-8 Fipronil-Sulfone 120068-36-2 Imazamethabenz-Methyl 81405-85-8

DDD, o,p- 53-19-0 Edifenphos 17109-49-8 Flamprop-Methyl 52756-25-9 Imibenconazole 86598-92-7

DDD, p,p- 72-54-8 Endosulfan, alpha- 959-98-8 Fluacrypyrim 229977-93-9 Imibenconazole-debenzyl 154221-27-9

DDE, o,p- 3424-82-6 Endosulfan, beta- 33213-65-9 Fluazifop-P-Butyl 79241-46-6 Indanofan 133220-30-1

Analytes CAS Analytes CAS Analytes CAS Analytes CAS

Indoxacarb 144171-61-9 Mevinphos 7786-34-7 Phosfolan 947-02-4 Pyridalyl 179101-81-6

Iodofenphos 18181-70-9 Mirex 2385-85-5 Phosmet 732-11-6 Pyridaphenthion 119-12-0

Iprobenfos 26087-47-8 Molinate 2212-67-1 Phosphamidon 13171-21-6 Pyrifenox 88283-41-4

Iprodione 36734-19-7 Monocrotophos 6923-22-4 Phoxim 14816-18-3 Pyrimethanil 53112-28-0

Iprodione, Impurity RP30228 63637-89-8 Myclobutanil 88671-89-0 Phthalide 87-41-2 Pyrimidifen 105779-78-0

Iprovalicarb 140923-17-7 Naled 300-76-5 Picolinafen 137641-05-5 Pyriminobac-methyl-E 136191-64-5

Isazofos 42509-80-8 Naphthylacetamide, 1- 86-86-2 Picoxystrobin 117428-22-5 Pyriminobac-methyl-Z 147411-70-9

Isocarbophos 24353-61-5 Naproanilide 52570-16-8 Piperonyl butoxide 51-03-6 Pyriproxyfen 95737-68-1

Isodrin 465-73-6 Napropamide 15299-99-7 Piperophos 24151-93-7 Pyroquilon 57369-32-1

Isofenphos 25311-71-1 Nicotine 54-11-5 Pirimicarb 23103-98-2 Quinalphos 13593-03-8

Isofenphos-Methyl 99675-03-3 Nitralin 4726-14-1 Pirimiphos-Ethyl 23505-41-1 Quinoclamine 2797-51-5

Isofenphos-oxon 31120-85-1 Nitrapyrin 1929-82-4 Pirimiphos-Methyl 29232-93-7 Quinoxyfen 124495-18-7

Isoprocarb 2631-40-5 Nitrofen 1836-75-5 Pretilachlor 51218-49-6 Quintozene 82-68-8

Isopropalin 33820-53-0 Nitrothal-Isopropyl 10552-74-6 Probenazole 27605-76-1 Quizalofop-Ethyl 76578-14-8

Isoprothiolane 50512-35-1 Norflurazon 27314-13-2 Prochloraz 67747-09-5 Resmethrin 10453-86-8

Isoxathion 18854-01-8 Nuarimol 63284-71-9 Procymidone 32809-16-8 Silafluofen 105024-66-6

Kresoxim-Methyl 143390-89-0 Omethoate 1113-02-6 Profenofos 41198-08-7 Simazine 122-34-9

Lenacil 2164-08-1 Orthophenylphenol 90-43-7 Prohydrojasmon 158474-72-7 Simeconazole 149508-90-7

Leptophos 21609-90-5 Orysastrobin 248593-16-0 Promecarb 2631-37-0 Simetryn 1014-70-6

Linuron 330-55-2 Oxabetrinil 74782-23-3 Prometon 1610-18-0 Spirodiclofen 148477-71-8

Malaoxon 1634-78-2 Oxadiazon 19666-30-9 Prometryn 7287-19-6 Spiromesifen 283594-90-1

Malathion 121-75-5 Oxadixyl 77732-09-3 Propachlor 1918-16-7 Spiroxamine 118134-30-8

MCPA-thioethyl 25319-90-8 Oxpoconazole fumarate 174212-12-5 Propamocarb 24579-73-5 Sulfallate 95-06-7

MCPB 94-81-5 oxy-Chlordane 27304-13-8 Propanil 709-98-8 Sulfotep 3689-24-5

MCPB-ethyl 10443-70-6 Oxyfluorfen 42874-03-3 Propaphos 7292-16-2 Sulprofos 35400-43-2

Mecarbam 2595-54-2 Paclobutrazol 76738-62-0 Propaquizafop 111479-05-1 Swep 1918-18-9

Mefenacet 73250-68-7 Paraoxon-Methyl 950-35-6 Propargite 2312-35-8 TCMTB 21564-17-0

Mefenpyr-Diethyl 135590-91-9 Parathion-ethyl 56-38-2 Propazine 139-40-2 Tebuconazole 107534-96-3

Mepanipyrim 110235-47-7 Parathion-Methyl 298-00-0 Propetamphos 31218-83-4 Tebufenpyrad 119168-77-3

Mepronil 55814-41-0 Pebulate 1114-71-2 Propham 122-42-9 Tebupirimfos 96182-53-5

Metalaxil-m 70630-17-0 Penconazole 66246-88-6 Propiconazole 60207-90-1 Tebuthiuron 34014-18-1

Metalaxyl 57837-19-1 Pencycuron 66063-05-6 Propisochlor 86763-47-5 Tecnazene 117-18-0

Metazachlor 67129-08-2 Pendimethalin 40487-42-1 Propoxur 114-26-1 Tefluthrin 79538-32-2

Methacrifos 62610-77-9 Pentachloroaniline 527-20-8 Propyzamide 23950-58-5 Temephos 3383-96-8

Methamidophos 10265-92-6 Pentachlorophenol 87-86-5 Proquinazid 189278-12-4 Terbacil 5902-51-2

Methidathion 950-37-8 Pentoxazone 110956-75-7 Prosulfocarb 52888-80-9 Terbucarb 1918-11-2

Methiocarb 2032-65-7 Permethrin, cis- 61949-76-6 Prothiofos 34643-46-4 Terbufos 13071-79-9

Methomyl-oxime 13749-94-5 Permethrin, trans- 61949-77-7 Pyraclofos 89784-60-1 Terbumeton 33693-04-8

Methoprene 40596-69-8 Perthane 72-56-0 Pyraclostrobin 175013-18-0 Terbuthylazine 5915-41-3

Methoxychlor 72-43-5 Phenmedipham 13684-63-4 Pyraflufen-Ethyl 129630-19-9 Terbutryn 886-50-0

Metolachlor 51218-45-2 Phenothrin 26002-80-2 Pyrazophos 13457-18-6 Tetrachlorvinphos 22248-79-9

Metolachlor-s 87392-12-9 Phenthoate 2597-03-7 Pyrazoxyfen 71561-11-0 Tetraconazole 112281-77-3

Metolcarb 1129-41-5 Phorate 298-02-2 Pyrethrins 8003-34-7 Tetradifon 116-29-0

Metominostrobin 133408-50-1 Phorate-Sulfone 2588-04-7 Pyributicarb 88678-67-5 Tetrahydrophthalimide 1469-48-3

Metribuzin 21087-64-9 Phosalone 2310-17-0 Pyridaben 96489-71-3 Tetramethrin 7696-12-0

Analytes CAS Analytes CAS Analytes CAS Analytes CAS

Thenylchlor 96491-05-3 Tolclofos-Methyl 57018-04-9 Trichlamide 70193-21-4 Triticonazole 131983-72-7

Thiabendazole 148-79-8 Tolfenpyrad 129558-76-5 Trichlorfon 52-68-6 Uniconazole-P 83657-17-4

Thiacloprid 111988-49-9 Tolylfluanid 731-27-1 Tricyclazole 41814-78-2 Vamidothion 2275-23-2

Thiamethoxam 153719-23-4 Tralomethrin 66841-25-6 Tridemorph 24602-86-6 Vamidothion sulfone 70898-34-9

Thifluzamide 130000-40-7 Triadimefon 43121-43-3 Trifloxystrobin 141517-21-7 Vinclozolin 50471-44-8

Thiobencarb 28249-77-6 Triadimenol 55219-65-3 Triflumizole 68694-11-1 XMC 2655-14-3

Thiocyclam-Oxalate 31895-22-4 Tri-Allate 2303-17-5 Triflumizole, FM-6-1 131549-75-2 Xylylcarb 2425-10-7

Thiometon 640-15-3 Triazophos 24017-47-8 Trifluralin 1582-09-8 Zoxamide 156052-68-5

Thionazin 297-97-2 Tribufos 78-48-8

Optional Internal Standards

Analytes CAS Analytes CAS Analytes CAS Analytes CAS

Acephate D6 2109699-73-0 Diethofencarb D7 Fosthiazate D5 Linuron D6 1219804-76-8

Carbaryl D7 362049-56-7 EPN D5 Imazalil D5 1398065-91-2 Metolachlor D6 1219803-97-0

Chlorpyrifos D10 285138-81-0 Esfenvalerate D7 Iprobenfos D7 Pendimethalin D5 1219803-39-0

Diazinon D10 100155-47-3 Etofenprox D5 Isoprothiolane D4 1715020-82-8 Thiabendazole 13C6

Dichlorvos D6 203645-53-8 Fenitrothion D6 203645-59-4 Isoxathion-D10

Simultaneous Analysis of 477 Residual Pesticides in Agricultural Crops Using GC-MS/MS Riki Kitano1, Masato Takakura1, Haruhiko Miyagawa1, Isao Saito2, Eiji Ueno3, Shilpi Chopra4, Robert H. Clifford4

1Shimadzu Corporation, 2Aichi Science & Technology Foundation, 3Aichi Prefectural Institute of Public Health, 4Shimadzu Scientific Instruments

Soy Brown Soy Brown Soy Brown ID Compound Name Orange Spinach ID Compound Name Orange Spinach ID Compound Name Orange Spinach Bean Rice Bean Rice Bean Rice 2-1: Analysis Conditions, continued 1 Aldicarb deg. 6.17 5.69 8.35 6.35 155 Dichlofluanid 8.82 3.70 11.00 4.75 309 Carbophenothion 6.80 6.19 9.12 5.47 Bifenazate in a Brown Rice Sample 1: Introduction 2 p-Dichlorobenzene 4.43 8.96 17.46 3.01 156 Esprocarb 3.96 2.87 16.08 6.33 310 Edifenphos 7.40 6.61 9.89 7.70 3 DCIP 6.28 9.40 15.28 3.52 157 Quinoclamine 8.61 6.58 9.51 7.30 311 Cyanofenphos 6.71 8.10 9.70 3.60 4 Methomyl oxime 5.83 8.16 18.02 2.35 158 Malathion 9.97 7.47 7.37 2.98 312 Norflurazon 2.59 3.51 9.22 5.22 5 Omethoate deg. 5.67 9.07 11.99 4.31 159 Metolachlor 3.77 3.78 12.20 4.78 313 Endosulfan sulfate 8.23 4.19 7.14 5.54 SH-Rxi-5Sil MS SH-Rtx-200MS GC-MS/MS systems can measure more than 400 residual pesticides in foods. However, analyzing Fig. 2: Analytical Program [Instrument] 6 Aldoxycarb deg. 5.88 8.96 12.97 7.69 160 Chlorpyrifos 7.22 3.28 9.78 6.08 314 2,4,5-T-2-ethylhexyl 8.52 8.28 8.91 9.38 (x10,000) (x1,000) 7 Clofentezine deg. 9.10 7.78 9.15 9.01 161 Thiobencarb 7.77 2.08 9.59 4.36 315 Propiconazole-1 9.10 8.66 8.72 7.01 2.00 8 Hymexazol 6.13 10.63 34.36 11.06 162 (Z)-Dimethylvinphos 3.07 4.28 11.45 2.37 316 Quinoxyfen 4.23 8.59 12.91 2.30 2.00 300.10>258.10 300.10>258.10 more than 400 pesticides simultaneously requires a short dwell time (data loading time) during MRM GC-MS: GCMS-TQ8040 (Twin Line MS System, Shimadzu) created by Smart MRM for 9 Methamidophos 4.82 7.66 8.84 1.86 163 Aldrin 4.05 7.01 15.68 7.76 317 Lenacil 3.52 8.95 8.93 3.65 Matrix Matrix 300.10>199.10 1.75 300.10>199.10 10 Dichlorvos 5.05 11.55 18.23 4.64 164 Diethofencarb 5.44 4.17 12.25 6.75 318 Trifloxystrobin 6.80 8.83 8.84 5.89 1.75

Brank Brank measurements, which results in problems with inadequate sensitivity and the tedious process of Column 1: SH-Rxi-5Sil MS (Length 30 m, 0.25 mm I.D., df=0.25 µm, Shimadzu) 11 Nereistoxin 4.15 9.24 15.44 9.07 165 Fenthion 4.11 5.06 9.33 5.36 319 Chloridazon 9.81 3.54 8.73 3.71 1.50 column 1 12 Allidochlor 5.59 6.36 7.14 9.19 166 Chlorthal-dimethyl 3.75 2.55 9.48 7.55 320 (E)-Pyriminobac-methyl 4.11 2.76 9.85 2.78 1.50 13 Dichlobenil 3.83 7.57 15.22 3.79 167 Cyanazine 9.63 8.73 6.89 5.13 321 Propiconazole-2 6.41 5.47 8.86 4.27 creating MRM measurement programs. Consequently, several different methods are used for target Column 2: SH-Rtx-200MS (Length 30 m, 0.25 mm I.D., df=0.25 µm, Shimadzu) 14 EPTC 5.19 4.14 15.04 5.95 168 Parathion 7.43 8.93 9.05 5.05 322 p,p'-DDT 8.83 4.76 14.63 9.39 1.25 1.25 15 Propamocarb 23.30 15.73 9.79 6.56 169 Fenpropimorph 2.29 4.36 5.56 5.01 323 Pyraflufen-ethyl 5.43 4.30 12.09 2.15 pesticides and the same sample is measured multiple times to analyze all components. That can Liner: Sky Liner, Splitless Single Taper Gooseneck w/Wool (Restek) 16 Biphenyl 4.04 3.97 15.94 3.40 170 Isofenphos oxon 4.01 3.54 10.86 4.06 324 Hexazinone 3.73 2.12 13.30 2.34 1.00 17 Mevinphos 3.03 3.17 13.44 3.75 171 Flufenacet 9.56 6.84 8.48 5.55 325 Thenylchlor 3.92 3.82 8.73 3.32 1.00 18 Vamidothion sulfone deg. 8.83 8.50 9.13 7.64 172 Isocarbophos 9.87 3.71 9.45 5.42 326 Tebuconazole 6.63 4.46 9.34 5.18 decrease productivity, due to the time required for analyzing all the components involved in the large Software: GCMSsolution Ver. 4.2 (Shimadzu) 19 Butylate 3.59 4.47 8.26 5.19 173 Triadimefon 5.66 7.30 9.52 9.15 327 Diclofop-methyl 4.48 2.59 13.83 3.84 0.75 0.75 20 Acephate 4.72 4.29 4.85 6.89 174 Tetraconazole 9.01 8.56 9.70 6.59 328 Propargite-1 4.20 7.86 8.13 3.56 number of pesticides being inspected. This Application Data Sheet describes a solution to these 21 Etridiazole 4.98 4.72 10.61 6.52 175 Carbetamide 5.42 6.18 8.57 6.89 329 Captafol 35.06 34.55 27.09 19.41 0.50 0.50 22 Nitrapyrin 4.17 5.44 9.65 6.47 176 Dicofol deg. 4.91 3.99 11.87 7.33 330 Propargite-2 3.43 5.70 8.66 9.12 23 Propham 4.55 3.84 15.43 3.13 177 Fthalide 6.92 5.01 12.09 4.79 331 Diflufenican 1.46 4.03 14.15 2.53 problems with the creation of a method for simultaneously analyzing 477 components and evaluating [GC] 24 Metolcarb 5.47 4.38 9.36 2.84 178 Nitrothal-isopropyl 9.08 5.82 9.61 7.88 332 Resmethrin-1 9.43 5.95 4.78 40.49 0.25 0.25 25 Methacrifos 5.07 6.91 8.93 5.83 179 Bromophos 6.20 3.00 8.63 5.62 333 Piperonyl butoxide 4.41 3.91 9.63 2.78 the resulting sensitivity and accuracy. Injection Temp.: 250 ﾟC 26 Clothianidin 3.66 4.84 7.53 2.92 180 Diphenamid 4.11 4.84 8.27 6.72 334 Resmethrin-2 5.05 3.06 8.27 6.02 0.00 27 Chloroneb 4.35 2.74 12.91 5.54 181 Fosthiazate-1 9.53 4.33 14.06 3.16 335 Epoxiconazole 4.26 4.19 9.00 4.66 25.50 25.75 26.00 26.25 23.50 23.75 24.00 24.25 Column Oven Temp: 60 ﾟC (1 min), 25 ﾟC/min to 160 ﾟC, 4 ﾟC/min to 240 ﾟC, 28 Crimidine 5.31 11.23 17.85 4.96 182 Fosthiazate-2 7.90 3.44 15.15 8.60 336 Bioresmethrin 4.72 1.63 8.77 17.85 29 Thiocyclam 5.33 9.22 14.54 3.52 183 Thiamethoxam deg. 8.72 17.07 14.95 4.44 337 Zoxamide 7.58 4.47 8.32 3.11 (x10,000) (x1,000) 30 Tebuthiuron 5.48 5.78 12.01 2.31 184 Isofenphos-methyl 5.22 5.36 8.33 4.60 338 Mefenpyr-diethyl 3.12 5.90 11.95 4.11 2.00 10 ﾟC/min to 290 ﾟC (11 min) 31 2-Phenylphenol 4.45 3.70 16.10 1.78 185 Pendimethalin 9.80 5.24 9.81 6.47 339 Pyributicarb 4.90 1.86 8.61 3.64 1.50 300.10>258.10 300.10>258.10 32 Isoprocarb 3.72 3.89 9.56 2.58 186 (E)-Chlorfenvinphos 1.74 4.88 8.85 5.48 340 Acetamiprid 7.56 7.09 8.10 3.44 300.10>199.10 Spiked 1.75 300.10>199.10 Spiked Injection Mode: Splitless (High Pressure Injection 250 kPa, 1.5 min) 33 Molinate 5.29 6.10 8.94 4.90 187 Cyprodinil 2.76 1.87 7.88 5.93 341 Chlomethoxyfen 9.35 7.60 9.21 7.28 2: Experiment

34 XMC 5.01 3.22 9.40 4.33 188 Fluazinam deg. 9.41 10.10 6.66 10.46 342 Pyridafenthion 4.72 6.51 9.91 6.04 1.25 Sample Sample 35 Xylylcarb 4.80 1.05 9.84 2.44 189 Anirazine 16.42 7.54 9.29 12.84 343 Iprodione 8.26 3.90 9.09 3.65 1.50 36 Tecnazene 6.90 4.93 8.45 4.47 190 Penconazole 8.07 7.19 9.19 6.48 344 Bromuconazole-1 6.97 1.42 7.21 1.70 Carrier Gas Control: Linear Velocity (40.0 cm/sec) 2-1: Sample Preparation 37 Fenobucarb 2.95 3.02 7.72 2.14 191 Fipronil 9.68 11.76 9.33 9.72 345 Carbosulfan 9.10 7.81 12.02 8.39 1.00 1.25 38 Propoxur 5.34 3.76 13.85 3.74 192 Heptachlor-exo-epoxide 5.92 13.72 8.89 7.93 346 Phosmet 8.47 5.64 7.72 7.42 Injection Volume: 2 µL 39 Propachlor 4.73 3.45 14.26 3.33 193 oxy-Chlordane 9.78 13.82 9.15 9.24 347 Tetramethrin-1 6.54 6.08 4.24 5.82 1.00 40 Alanycarb deg. 9.64 7.43 16.80 6.50 194 Dimethametryn 6.00 9.07 9.52 2.05 348 EPN 7.68 9.03 5.85 7.96 0.75 41 Etobenzanid N.D. N.D. N.D. N.D. 195 Chlozolinate 9.18 9.01 9.97 8.44 349 Bromopropylate 4.58 3.65 9.49 3.71 Figure in parentheses is Matrix solutions were prepared by processing soy bean, orange, brown rice, and spinach samples 42 Chlorethoxyphos 7.08 5.79 7.83 4.05 196 Tolylfluanid 9.08 7.84 4.04 5.84 350 Chlorantraniliprole 9.05 7.05 8.94 9.56 0.75 %RSD 43 Demeton-S-methyl 4.74 3.38 9.33 3.99 197 (Z)-Pyrifenox 8.62 8.85 7.31 8.57 351 Piperophos 4.82 5.86 8.86 3.37 0.50 dwell time for 1 ch [MS] according to a pretreatment procedure for residual pesticide analysis, and then purifying them using 44 Diphenylamine 5.41 2.62 15.30 3.86 198 Phosfolan 6.03 10.06 9.36 11.04 352 Bifenthrin 4.52 2.87 8.77 2.67 0.50 = 8.44 % 45 Ethoprophos 6.21 5.12 16.16 3.08 199 Isofenphos 5.78 12.21 8.22 7.73 353 Picolinafen 7.00 6.88 8.52 3.20 1) Interface Temp.: 300 ﾟC 46 Phenmedipham deg. 5.52 5.01 10.93 3.14 200 (Z)-Chlorfenvinphos 5.91 10.86 9.18 3.64 354 Tetramethrin-2 7.23 3.31 8.15 4.44 0.25 the GPC Cleanup System (from Shimadzu Corporation). Measurement sample solutions (1 g/mL 47 Ethalfluralin 9.13 7.91 5.90 8.18 201 Allethrin-1 8.77 8.27 7.32 7.45 355 Fenoxycarb 8.39 5.20 9.83 3.32 0.25 48 Naled 8.73 9.73 8.02 6.57 202 Ethychlozate 4.82 9.11 9.20 3.67 356 Bifenazate 9.05 7.67 9.58 6.96 ﾟ sample concentration) were then prepared by spiking the prepared matrix solutions with 477 Ion Source Temp.: 200 C 49 Chlorpropham 4.65 3.06 13.43 4.30 203 Mecarbam 9.53 6.32 14.87 9.37 357 Methoxychlor 10.38 3.19 9.34 9.91 0.00 50 Dicrotophos 2.85 3.93 8.97 5.05 204 Thiabendazole 4.22 3.90 9.34 5.03 358 Etoxazole 8.36 8.97 7.73 11.37 25.50 25.75 26.00 26.25 23.50 23.75 24.00 24.25 Measurement Mode: MRM 51 Dichlofluanid metabolite 4.76 4.20 14.91 3.38 205 Captan 14.99 3.41 5.66 10.28 359 Fenamidone 7.08 6.98 13.32 4.69 components (including internal standard substances) to a concentration of 5 ppb (or 200 ppb for the 52 Flusilazole metabolite 5.74 2.48 9.58 5.47 206 Diclocymet-1 7.87 7.38 9.82 7.31 360 Fenpropathrin 8.53 4.96 9.79 9.01 ※ 53 Trifluralin 9.90 2.41 8.55 2.35 207 Phenthoate 8.96 1.92 9.77 5.60 361 Indanofan 7.44 4.67 5.18 9.50 internal standard substances). 19 kinds of surrogate pesticides were used as the internal standard Loop Time: 0.4 sec 54 Bendiocarb 5.10 4.27 9.56 4.63 208 Quinalphos 6.63 5.12 8.38 7.46 362 Anilofos 5.80 7.00 8.62 4.17 55 Benfluralin 7.43 4.69 8.37 5.35 209 Allethrin-2 6.35 8.84 8.28 9.50 363 Bromuconazole-2 8.14 7.98 6.65 2.91 Processing Time: 0.3 min 56 Dioxabenzofos 5.17 3.74 8.54 1.94 210 Dimepiperate 4.66 5.69 12.28 5.23 364 Tebufenpyrad 3.66 4.18 9.73 2.88 Clothianidin in a Spinach Sample substances. 57 Sulfotep 6.39 2.81 7.68 3.98 211 Triadimenol-1 3.25 3.68 7.58 6.15 365 Orysastrobin 9.60 5.23 9.51 3.46 58 Monocrotophos 5.14 4.67 8.22 9.31 212 Procymidone 4.31 5.49 12.58 5.87 366 Bifenox 8.66 8.37 14.87 2.48 59 Cadusafos 4.47 2.99 8.37 4.92 213 Folpet 13.90 6.98 20.67 11.30 367 Clomeprop 3.08 4.93 7.61 0.94 60 Promecarb 3.95 3.20 8.75 4.01 214 Triflumizole 7.71 7.73 7.93 8.74 368 Etoxazole metabolite 3.77 10.39 9.82 6.57 SH-Rxi-5Sil MS SH-Rtx-200MS 61 Di-allate-1 4.62 2.99 7.81 5.05 215 (Z)-Ferimzone 5.61 7.92 17.83 8.84 369 Furametpyr 4.75 6.54 9.95 4.31 (x1,000) (x1,000) 62 Phorate 6.20 4.87 10.62 4.72 216 Triadimenol-2 4.79 7.19 8.83 6.81 370 Tetradifon 8.47 4.02 8.83 7.17 63 alpha-BHC 5.36 4.87 12.66 4.14 217 Methidathion 4.97 4.72 14.05 4.25 371 Phenothrin-1 7.07 5.10 5.39 6.48 2.5 3: Results 132.00>71.00 132.00>71.00 64 Di-allate-2 6.63 3.99 7.29 6.45 218 Methoprene 4.34 5.97 8.88 8.61 372 Furathiocarb 9.09 8.69 12.79 5.84 132.00>45.00 Matrix 1.00 132.00>45.00 Matrix Sample 10g 65 Hexachlorobenzene 5.83 3.78 12.13 2.77 219 Chlorbenside 5.54 5.96 14.34 3.56 373 Iprodione metabolite 10.15 6.54 12.65 9.65 66 Thiometon 5.08 6.09 15.22 3.48 220 trans-Chlordane 2.84 3.86 13.37 3.85 374 Triticonazole 7.76 8.80 5.99 5.19

Brank Brank 67 Desmedipham deg. 3.90 6.72 8.71 5.10 221 Bromophos-ethyl 4.56 6.15 8.78 2.26 375 Phosalone 7.83 7.29 7.37 3.64 2.0 68 Dimethoate 7.03 5.21 8.75 6.91 222 Chinomethionat 7.98 4.48 11.60 1.82 376 Phenothrin-2 6.89 3.49 5.19 2.93 3-1. Consideration of Analysis Method 69 Dichloran 8.81 5.78 9.28 4.36 223 Diclocymet-2 8.79 8.86 11.31 5.28 377 Leptophos 6.33 5.02 8.74 5.49 0.75 70 Carbofuran 7.07 3.49 7.56 4.94 224 Propaphos 6.79 3.90 9.65 4.20 378 Azinphos-methyl 7.95 8.06 8.35 5.72 1.5 71 Simazine 5.92 6.09 7.77 4.22 225 (E)-Pyrifenox 9.74 6.86 7.75 9.37 379 Pentoxazone 4.16 3.49 9.30 3.96 72 Furilazole 8.41 5.85 9.90 7.85 226 Vamidothion 8.10 6.71 8.09 11.32 380 Pyriproxyfen 4.25 6.00 5.30 3.39 Dispense and extract with hexane and acetonitrile under 73 beta-BHC 7.32 4.99 8.24 3.45 227 Phoxim N.D. N.D. N.D. N.D. 381 Mefenacet 2.62 3.85 9.36 2.88 0.50 74 Atrazine 7.66 5.71 14.05 4.47 228 Tetrachlorvinphos 3.37 5.35 14.75 6.51 382 Cyhalothrin-1 6.70 4.53 6.38 8.98 1.0 75 Dimethipin 6.42 8.25 8.70 3.11 229 Paclobutrazol 5.52 2.81 9.80 4.82 383 Cyhalofop-butyl 7.55 3.47 8.67 5.35 acetic acid condition 76 Swep 5.85 4.93 10.38 3.86 230 Trichlamide 7.78 3.23 9.93 5.82 384 Furametpyr metabolite 5.55 4.62 6.92 5.44 77 Chlorbufam 9.56 8.75 8.81 5.12 231 Disulfoton sulfone 9.06 5.95 13.64 3.33 385 Mirex 5.07 3.93 10.60 3.54 0.25 78 Triflumizole metabolite 6.74 4.05 11.09 6.52 232 Butachlor 9.05 5.75 8.79 5.25 386 Amitraz 7.20 6.30 9.82 7.03 0.5 79 Clomazone 4.77 4.91 8.49 4.26 233 Fenothiocarb 4.58 4.80 13.85 2.36 387 lambda-Cyhalothrin 7.16 5.09 7.92 5.04 80 Propazine 4.75 4.22 7.02 5.85 234 alpha-Endosulfan 8.92 3.48 9.39 3.12 388 Cyhalothrin-2 7.16 5.09 7.89 5.04 81 Pentachlorophenol 9.28 9.36 7.41 7.15 235 cis-Chlorden 8.37 9.04 6.83 4.39 389 Fenarimol 1.45 4.13 9.64 2.72 0.00 82 Quintozene 9.92 6.60 9.10 7.69 236 Ditalimfos 7.12 5.37 9.84 5.20 390 Pyrazophos 7.14 3.08 5.34 4.98 7.50 7.75 8.00 8.25 7.25 7.50 7.75 8.00 83 Tolylfluanid metabolite 7.09 5.06 14.90 5.08 237 Mepanipyrim 4.63 3.89 9.55 3.77 391 Naproanilide 3.24 3.17 8.85 3.36 84 Aminocarb 6.02 6.40 9.35 5.45 238 Imazamethabenz-methyl ester-1 7.72 10.24 8.72 5.49 392 Benfuracarb 6.65 11.04 8.49 4.62

Collect acetonitrile part and salt out 85 gamma-BHC 9.73 3.38 9.13 7.18 239 Butamifos 9.69 2.76 8.39 4.09 393 Azinphos-ethyl 9.00 8.28 6.64 4.31 (x1,000) (x1,000) 86 Propetamphos 8.44 5.74 7.23 4.31 240 Imazamethabenz-methyl ester-2 16.61 7.98 8.88 8.37 394 Acrinathrin 5.27 9.02 8.37 8.17 2.5 132.00>71.00 132.00>71.00 87 Cyanophos 5.89 3.82 8.64 4.01 241 Flutriafol 7.21 2.86 9.34 6.04 395 Dialifos 7.25 4.14 7.81 3.50 88 Terbufos 2.89 4.37 7.94 5.04 242 TCMTB 9.36 5.33 9.39 4.53 396 Pyraclofos 6.95 7.89 8.62 8.51 132.00>45.00 Spiked 1.00 132.00>45.00 Spiked 89 2,4,5-T-methyl 5.60 7.15 14.65 5.32 243 Napropamide 3.71 4.46 8.37 3.06 397 Fenoxaprop-ethyl 2.86 5.90 8.23 3.90 Sample Sample 90 Fonofos 4.34 2.90 13.12 5.14 244 Fenamiphos 8.37 5.98 8.79 3.42 398 Oryzalin N.D. 32.85 39.81 18.39 2.0 91 Propyzamide 8.45 2.04 7.78 3.32 245 Chlorfenson 3.71 2.60 10.09 5.59 399 Spirodiclofen 8.85 6.78 6.17 6.25 Fig. 3: Relationship Between Retention Time 92 Pyroquilon 9.00 3.08 7.76 4.18 246 Hexaconazole 5.49 8.17 8.81 5.20 400 Bitertanol-1 8.32 6.08 9.23 4.32 0.75 93 Diazinon 8.13 4.68 9.35 7.42 247 Imazalil 8.84 5.09 8.01 4.24 401 Permethrin-1 7.47 8.39 5.67 4.96 Re-dissolve in ethyl acetate and dehydrate, filtrate and Dwell Time (for retention times from 5 to 94 Phosphamidon-1 11.13 9.14 8.29 9.42 248 Flutolanil 4.88 3.61 9.69 1.93 402 Bitertanol-2 8.56 7.68 5.18 6.37 1.5 95 Pyrimethanil 2.80 3.38 8.13 5.52 249 Tricyclazole 7.36 2.13 5.22 4.20 403 Coumaphos 5.15 6.18 7.79 4.18 30 minutes) 96 Chlorothalonil N.D. N.D. 16.62 12.90 250 (E)-Metominostrobin 6.56 7.28 9.20 4.55 404 Fluquinconazole 4.59 3.44 8.88 4.42 0.50 97 Disulfoton 9.89 5.61 8.96 7.06 251 Fludioxonil 5.37 2.82 13.73 3.51 405 Pyridaben 6.42 3.16 7.25 1.59 1.0 %RSD 98 Isazophos 4.10 7.79 8.88 5.05 252 Prothiofos 9.31 4.77 10.21 4.80 406 Permethrin-2 4.72 6.31 7.92 4.83 99 Prohydrojasmon-1 8.29 4.13 9.09 4.79 253 Isoprothiolane 3.65 4.46 8.04 5.86 407 Dioxathion deg. 7.04 6.73 6.70 3.66 = 7.33 % 100 Terbacil 8.28 3.45 9.52 4.26 254 Pretilachlor 7.25 1.66 15.63 6.15 408 Prochloraz 5.36 5.50 4.66 3.53 0.25 101 delta-BHC 6.63 6.04 9.64 6.84 255 Profenofos 2.63 6.41 14.14 5.90 409 Oxpoconazole fumarate 7.06 5.41 7.45 3.04 0.5 102 Tefluthrin 3.77 4.24 12.66 3.00 256 p,p'-DDE 3.72 8.10 13.07 4.14 410 Butafenacil 3.99 2.59 8.62 3.42 Defat and clean up with GPC 103 Etrimfos 9.89 9.07 8.98 8.29 257 Uniconazole-P 4.64 9.02 9.38 3.92 411 Cafenstrole 5.85 9.17 8.80 5.94 104 Tri-allate 5.86 11.83 11.03 2.58 258 Dieldrin 9.55 8.16 9.39 6.59 412 Fenbuconazole 3.84 12.80 8.65 2.60 105 MCPA-thioethyl 8.96 10.15 9.79 2.35 259 Oxadiazon 7.30 8.45 17.44 4.57 413 Cyfluthrin-1 7.05 11.97 7.44 8.83 0.00 106 Tebupirimfos 6.99 7.22 10.46 3.56 260 Tribuphos 7.58 7.95 9.31 10.01 414 Cyfluthrin-2 7.24 14.88 8.12 7.69 7.50 7.75 8.00 8.25 7.25 7.50 7.75 8.00 The average dwell time for all components was 12.3 msec, with over 6.5 msec provided even for retention time bands 107 Pirimicarb 3.76 4.48 14.58 2.32 261 Thifluzamide 9.01 5.91 13.36 6.33 415 Cyfluthrin-3 8.03 13.13 6.64 7.74 108 Iprobenfos 3.77 3.83 12.89 3.25 262 Myclobutanil 4.80 5.72 9.55 2.11 416 Cyfluthrin-4 7.78 12.49 8.46 8.92 where a high number of pesticides were eluted by Smart MRM. 109 Prohydrojasmon-2 14.40 5.90 7.10 8.17 263 Flamprop-methyl 7.13 8.55 8.44 3.72 417 Cypermethrin-1 8.23 8.70 7.71 1.42 110 Oxabetrinil 8.83 9.12 12.93 8.47 264 o,p'-DDD 5.51 3.71 11.30 3.02 418 Boscalid 5.29 14.34 9.02 3.51 Fig. 5 Matrix Separation by Twin Line MS System 111 Benoxacor 7.31 1.86 8.91 4.25 265 Iprovalicarb-1 4.63 2.99 11.44 5.59 419 Halfenprox 8.56 8.27 8.47 1.74 Fig. 1: Procedure of sample 112 Formothion 9.59 9.30 8.87 9.35 266 Flusilazole 7.51 7.35 8.85 5.56 420 Cypermethrin-2 8.68 5.80 8.49 7.71 Clean up additionally with SAX/PSA cartridge 113 Ethiofencarb 5.46 3.77 16.07 7.26 267 Imibenconazole-debenzyl 8.79 5.70 9.00 4.25 421 Cypermethrin-3 9.28 5.31 8.79 5.44 preparation 114 MCPB-ethyl 4.95 4.38 12.23 2.04 268 Carboxin 7.48 6.39 14.71 4.33 422 Quizalofop-ethyl 3.81 10.78 8.88 2.55 3-2. Analytical Accuracy 115 Phosphamidon-2 6.31 8.94 14.90 6.40 269 Diclobutrazol 5.49 4.96 9.61 3.27 423 Flucythrinate-1 8.64 12.75 7.38 2.22 116 Benfuresate 4.35 3.92 7.14 6.73 270 Buprofezin 3.52 7.26 14.13 3.63 424 Cypermethrin-4 4.59 12.36 2.67 7.80 117 Dichlofenthion 3.01 1.45 9.06 2.19 271 Oxyfluorfen 13.68 10.43 7.74 5.88 425 Etofenprox 4.72 7.17 7.04 3.51 118 Dimethenamid 5.71 3.00 13.55 3.43 272 Bupirimate 4.36 5.60 8.99 3.87 426 Pyridalyl 5.93 8.43 5.20 4.39 119 Propanil 5.41 5.47 16.78 2.89 273 Azaconazole 4.95 4.43 9.17 4.74 427 Flucythrinate-2 7.65 9.06 7.34 3.59 4. Conclusion Th show that %RSD (n = 5) was 10 % or 120 Terbucarb 3.13 4.64 12.30 1.63 274 (Z)-Metominostrobin 5.47 5.06 8.20 1.92 428 Silafluofen 3.09 10.17 8.81 2.84 is s 121 Acetochlor 6.74 6.94 8.74 3.01 275 Kresoxim-methyl 6.77 6.34 13.15 3.58 429 Fluridone 4.18 8.52 7.62 7.33 less for 88 % of targets (1618 of the 1832 122 Metribuzin 6.76 4.37 8.83 5.94 276 Iprovalicarb-2 5.84 5.03 14.63 5.34 430 Thiacloprid 5.04 6.68 8.10 7.22 • High analytical accuracy was achieved when analyzing as many as 477 2-1: Analysis Conditions 123 Chlorpyrifos-methyl 5.96 4.24 16.12 6.56 277 Chlorfenapyr 10.54 7.30 5.37 7.93 431 Pyrimidifen 5.88 9.43 7.33 4.18 124 Bromobutide 9.13 8.11 9.03 4.47 278 Cyproconazole-1 8.97 8.01 6.76 4.03 432 Flumioxazin 7.68 18.20 6.20 7.89 components in four types of matrix), which 125 2,4-D-butyl 4.14 7.76 4.95 6.76 279 Cyproconazole-2 3.53 4.40 8.74 6.18 433 Esfenvalerate-1 7.53 11.25 8.79 7.42 components simultaneously. 126 Vinclozolin 9.04 4.66 9.79 9.10 280 Isoxathion 9.10 7.85 12.21 9.12 434 Fenvalerate-1 8.28 14.86 9.21 6.41 127 Parathion-methyl 7.86 4.91 7.77 3.41 281 Cyflufenamid 7.58 4.13 7.55 8.87 435 Pyraclostrobin 4.69 14.94 8.83 4.70 The GCMS-TQ8040 combined with the Twin Line MS System was used to measure samples based indicates that high analytical accuracy was 128 Simeconazole 5.27 2.92 7.67 9.71 282 Endrin 7.67 9.25 16.10 8.54 436 Fluvalinate-1 7.30 18.56 7.66 4.07 • By eliminating the need to split the analysis using multiple methods, the 129 Alachlor 5.12 4.40 8.15 5.08 283 Fenoxanil 3.33 7.39 7.65 7.25 437 Fenvalerate-2 8.60 16.74 8.23 4.30 on the following analytical conditions. Two transitions were specified for each component, one for achieved when analyzing 477 130 Spiroxamine-1 6.32 3.23 8.88 2.64 284 Perthane 6.29 4.96 14.83 2.13 438 Esfenvalerate-2 8.48 15.21 8.82 8.93 number of injections is reduced and productivity increased. This also as many as 131 Tolclofos-methyl 8.51 7.87 8.79 1.95 285 Fluazifop-P-butyl 2.57 5.82 9.86 5.51 439 Fluvalinate-2 9.36 14.88 8.74 4.08 132 Carbaryl 4.44 8.21 8.83 6.73 286 (E)-Ferimzone 8.97 10.86 9.14 10.56 440 Difenoconazole-1 1.52 9.27 7.86 2.83 quantitation and the other for confirmation, and Smart MRM was used to automatically create a components simultaneously. 133 Simetryn 8.36 1.91 8.06 3.60 287 Chloropropylate 5.02 3.22 10.87 3.38 441 Difenoconazole-2 5.84 9.25 7.16 7.27 allows maintenance frequency and costs to be minimized 134 Acibenzolar-S-methyl 7.89 9.62 7.68 3.21 288 Chlorobenzilate 4.54 2.76 13.87 1.81 442 Pyrazoxyfen 13.03 4.55 6.98 11.78 measurement program. 135 Heptachlor 7.92 3.29 8.59 4.05 289 beta-Endosulfan 8.66 8.25 12.65 4.06 443 Deltamethrin-1 7.47 10.84 5.80 9.68 136 Ametryn 6.62 3.36 9.48 3.15 290 (Z)-Pyriminobac-methyl 4.76 2.80 9.63 3.08 444 Tralomethrin deg.-1 7.47 14.13 7.60 11.31 137 Metalaxyl 2.88 6.82 14.92 5.22 291 Fensulfothion 6.45 8.97 6.32 7.70 445 Indoxacarb 1.55 16.56 7.02 7.07 Due to matrix interference, some 138 Mefenoxam 5.97 3.71 14.34 6.13 292 Flufenpyr-ethyl 6.51 8.81 9.49 5.68 446 Deltamethrin-2 8.96 13.34 7.63 6.82 5. References 139 Fenchlorphos 9.60 2.81 8.53 4.35 293 Diniconazole 6.11 5.13 9.49 6.16 447 Tralomethrin deg.-2 9.19 12.97 8.67 6.57 140 Cinmethylin 9.81 9.70 9.79 9.88 294 Oxadixyl 4.29 5.10 16.87 2.65 448 Azoxystrobin 5.01 4.22 4.54 5.80 pesticide peaks cannot be detected 141 Prometryn 4.48 5.90 8.83 7.87 295 p,p'-DDD 5.96 3.05 15.37 3.31 449 Flumiclorac-pentyl 6.70 12.85 7.63 4.23 142 Dithiopyr 6.05 4.76 8.81 3.18 296 Ethion 5.81 4.81 9.01 4.22 450 Dimethomorph-1 3.07 10.04 8.24 3.27 1) E. Ueno, et al., J. AOAC INT. 87, (2004) 1003-1015 properly with column 1, but using 143 Demeton-S-methylsulphon 3.03 7.07 8.59 5.83 297 o,p'-DDT 7.99 5.29 13.99 6.44 451 Famoxadone 5.25 16.17 8.35 4.57 144 2-(1-Naphthyl)acetamide 5.59 4.31 5.93 6.28 298 Fluacrypyrim 7.06 8.28 9.50 6.28 452 Tolfenpyrad 1.51 9.54 7.29 4.73 145 Pirimiphos-methyl 7.24 9.41 9.11 6.64 299 Mepronil 4.74 4.75 15.28 4.94 453 Dimethomorph-2 2.43 10.16 7.65 4.29 column 2 allows separation of the matrix 146 Terbutryn 5.85 4.68 9.84 4.18 300 Triazophos 6.42 4.64 8.64 2.63 454 Imibenconazole 7.22 4.06 4.91 4.96 147 Fenitrothion 9.87 6.55 5.77 7.20 301 Sulprofos 7.96 4.26 9.33 8.81 455 Cinidon-ethyl 3.60 4.10 8.06 4.02 ※Dichlorvos-d6, Acephate-d6, Diazinon-d10, Iprobenfos-d7, Carbaryl and results in accurate detection. 148 Bromacil 2.98 5.84 7.18 6.26 302 Azamethiphos 5.53 4.87 8.07 8.63 456 Fluthiacet-methyl 2.18 2.20 7.01 4.68 149 Methiocarb 3.98 8.09 9.01 9.34 303 Famphur 6.59 4.54 14.32 3.93 457 Propaquizafop 7.22 3.85 8.81 9.02 -d , Fenitrothion-d , Linuron-d , Metolachlor-d , Chlorpyrifos-d , 150 Ethofumesate 5.91 3.73 12.67 5.13 304 Pyrethrin I 34.71 14.52 5.34 15.27 458 Temephos 7.47 9.56 7.46 5.86 7 6 6 6 10 Furthermore, high-precision analytical 151 Spiroxamine-2 5.16 5.66 7.01 7.22 305 Chlornitrofen 9.44 8.24 19.05 8.60 Number of Components 440 406 334 438 152 Probenazole 9.51 8.48 7.12 9.37 306 Benalaxyl 4.19 4.60 5.60 2.75 with 10 % or Lower Diethofencarb-d7, Fosthiazate-d5, 153 (E)-Dimethylvinphos 6.49 7.09 13.92 5.65 307 Isoxadifen-ethyl 7.68 8.37 8.67 2.54 Average %RSD results can be obtained even when using 6.62 6.46 9.90 5.55 13 Fig. 4: %RSD Distribution for Each Matrix 154 Linuron 7.87 6.27 13.16 4.65 308 Carfentrazone-ethyl 8.05 3.45 9.33 3.74 (excluding N.D.) Pendimethalin-d , Thiabendazole- C , Imazalil-d , column 2. 5 6 5 soprothiolane-d , soxathion-d , EPN-d , tofenprox-d , and Table 1 %RSD (n = 5) of Samples Spiked with Pesticides (5 ppb) I 4 I 10 5 E 5 Esfenvalerate-d7 Items determined to have 20 % or more overlap (area values) between pesticide-spiked and blank samples are underlined (reference data). Excludes the 19 internal standard substances.