PRKAB2 Antibody Cat. No.: 14-931

PRKAB2 Antibody

Immunohistochemistry of paraffin- embedded rat brain using PRKAB2 Immunohistochemistry of paraffin-embedded mouse brain using PRKAB2 Antibody (14-931) at dilution of 1:100 Antibody (14-931) at dilution of 1:100 (40x lens). (40x lens).

Immunofluorescence analysis of U2OS cells using PRKAB2 antibody (14-931). Blue: DAPI for nuclear staining.

Specifications

October 1, 2021 1 https://www.prosci-inc.com/prkab2-antibody-14-931.html HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of IMMUNOGEN: human PRKAB2 (NP_005390.1).

TESTED APPLICATIONS: IF, IHC, WB

WB: ,1:500 - 1:2000

APPLICATIONS: IHC: ,1:50 - 1:200

IF: ,1:50 - 1:200

POSITIVE CONTROL: 1) MCF7

2) Mouse heart

3) Mouse brain

4) Rat brain

PREDICTED MOLECULAR Observed: 35kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: PRKAB2

5'-AMP-activated protein kinase subunit beta-2, 5'-AMP-activated protein kinase, beta-2 subunit, AMP-activated protein kinase beta 2 non-catalytic subunit, AMPK beta 2, AMPK ALTERNATE NAMES: beta-2 chain, AMPK subunit beta-2, protein kinase, AMP-activated, beta 2 non-catalytic subunit

GENE ID: 5565

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

October 1, 2021 2 https://www.prosci-inc.com/prkab2-antibody-14-931.html Background and References

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non- catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and BACKGROUND: beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.

ANTIBODIES FOR RESEARCH USE ONLY.

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