PRKAB2 Antibody Cat. No.: 14-931 PRKAB2 Antibody Immunohistochemistry of paraffin- embedded rat brain using PRKAB2 Immunohistochemistry of paraffin-embedded mouse brain using PRKAB2 Antibody (14-931) at dilution of 1:100 Antibody (14-931) at dilution of 1:100 (40x lens). (40x lens). Immunofluorescence analysis of U2OS cells using PRKAB2 antibody (14-931). Blue: DAPI for nuclear staining. Specifications October 1, 2021 1 https://www.prosci-inc.com/prkab2-antibody-14-931.html HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of IMMUNOGEN: human PRKAB2 (NP_005390.1). TESTED APPLICATIONS: IF, IHC, WB WB: ,1:500 - 1:2000 APPLICATIONS: IHC: ,1:50 - 1:200 IF: ,1:50 - 1:200 POSITIVE CONTROL: 1) MCF7 2) Mouse heart 3) Mouse brain 4) Rat brain PREDICTED MOLECULAR Observed: 35kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: PRKAB2 5'-AMP-activated protein kinase subunit beta-2, 5'-AMP-activated protein kinase, beta-2 subunit, AMP-activated protein kinase beta 2 non-catalytic subunit, AMPK beta 2, AMPK ALTERNATE NAMES: beta-2 chain, AMPK subunit beta-2, protein kinase, AMP-activated, beta 2 non-catalytic subunit GENE ID: 5565 USER NOTE: Optimal dilutions for each application to be determined by the researcher. October 1, 2021 2 https://www.prosci-inc.com/prkab2-antibody-14-931.html Background and References The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non- catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and BACKGROUND: beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 3 https://www.prosci-inc.com/prkab2-antibody-14-931.html.