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Tel:240-252-7368(USA) Fax:240-252-7376(USA) www.elabscience.com ® E-mail:[email protected] Elabscience Elabscience Biotechnology Inc. HBE1 Polyclonal Antibody Catalog No. E-AB-18898 Reactivity H Storage Store at -20℃. Avoid freeze / thaw cycles. Host Rabbit Applications WB,IHC,ELISA Isotype IgG Note: Centrifuge before opening to ensure complete recovery of vial contents. Images Immunogen Information Immunogen Fusion protein of human HBE1 Gene Accession BC015537 Swissprot P02100 Synonyms Epsilon-globin,HBE,HBE1,Hemoglobin epsilon chain,Hemoglobin subunit epsilon Western blot analysis of Human Product Information placenta tissue lysate using HBE1 Calculated MW 16 kDa Polyclonal Antibody at dilution of Observed MW Refer to figures 1:300 Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 Purify Antigen affinity purification Dilution WB 1:500-1:2000, IHC 1:30-1:150, ELISA 1:5000-1:10000 Background The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin Immunohistochemistry of paraffin- genes are expressed in a tightly developmentally controlled fashion.ε- embedded Human tonsil tissue using globin (HBE1) is the predominantly expressed gene during the embryonic HBE1 Polyclonal Antibody at dilution stage. The epsilon hemoglobin chain seems to be the best marker for fetal of 1:30(×200) nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains. For Research Use Only Focus on your research Thank you for your recent purchase. If you would like to learn more about antibodies,please visit www.elabscience.com. Service for life science Applications:WB-Western Blot IHC-Immunohistochemistry IF-Immunofluorescence IP-Immunoprecipitation FC-Flow cytometry ChIP- Chromatin Immunoprecipitation Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh- Sheep Pg-Pig Z-Zebrafish X-Xenopus C-Cow. Powered by TCPDF (www.tcpdf.org).

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Diagnosis of Sickle Cell Disease and HBB Haplotyping in the Era of Personalized Medicine: Role of Next Generation Sequencing
Journal of Personalized Medicine Article Diagnosis of Sickle Cell Disease and HBB Haplotyping in the Era of Personalized Medicine: Role of Next Generation Sequencing Adekunle Adekile 1,*, Nagihan Akbulut-Jeradi 2, Rasha Al Khaldi 2, Maria Jinky Fernandez 2 and Jalaja Sukumaran 1 1 Department of Pediatrics, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait; jalajasukumaran@hotmail 2 Advanced Technology Company, Hawali 32060, Kuwait; [email protected] (N.A.-J.); [email protected] (R.A.); [email protected] (M.J.F.) * Correspondence: [email protected]; Tel.: +965-253-194-86 Abstract: Hemoglobin genotype and HBB haplotype are established genetic factors that modify the clinical phenotype in sickle cell disease (SCD). Current methods of establishing these two factors are cumbersome and/or prone to errors. The throughput capability of next generation sequencing (NGS) makes it ideal for simultaneous interrogation of the many genes of interest in SCD. This study was designed to confirm the diagnosis in patients with HbSS and Sβ-thalassemia, identify any ß-thal mutations and simultaneously determine the ßS HBB haplotype. Illumina Ampliseq custom DNA panel was used to genotype the DNA samples. Haplotyping was based on the alleles on five haplotype-specific SNPs. The patients studied included 159 HbSS patients and 68 Sβ-thal patients, previously diagnosed using high performance liquid chromatography (HPLC). There was Citation: Adekile, A.; considerable discordance between HPLC and NGS results, giving a false +ve rate of 20.5% with a S Akbulut-Jeradi, N.; Al Khaldi, R.; sensitivity of 79% for the identification of Sβthal.
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Gene Expression Profiling of Peripheral Blood in Patients With
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Evolution of hemoglobin loci and their regulatory elements Sjaak Philipsen1 and Ross C. Hardison2 1 Department of Cell Biology Ee1071b, Erasmus MC, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands. Email [email protected] Tel. +31-10-7044282 2 Department of Biochemistry and Molecular Biology, Huck Institute for Comparative Genomics and Bioinformatics, The Pennsylvania State University, University Park, PA 16802, USA. Email [email protected] Tel. +1-814-8630113 Key words: transcriptional regulation; globin genes; locus control regions; super-enhancers; evolution Word count Abstract: 178 Review: 5793 This article was accepted to Blood Cells, Molecules and Diseases in August 2017. The DOI is doi.org/10.1016/j.bcmd.2017.08.001 The link to the article at BCMD is https://doi.org/10.1016/j.bcmd.2017.08.001 1 Abstract Across the expanse of vertebrate evolution, each species produces multiple forms of hemoglobin in erythroid cells at appropriate times and in the proper amounts. The multiple hemoglobins are encoded in two globin gene clusters in almost all species. One globin gene cluster, linked to the gene NPRL3, is preserved in all vertebrates, including a gene cluster encoding the highly divergent globins from jawless vertebrates. This preservation of synteny may reflect the presence of a powerful enhancer of globin gene expression in the NPRL3 gene. Despite substantial divergence in noncoding DNA sequences among mammals, several epigenetic features of the globin gene regulatory regions are preserved across vertebrates. The preserved features include multiple DNase hypersensitive sites, at least one of which is an enhancer, and binding by key lineage-restricted transcription factors such as GATA1 and TAL1, which in turn recruit coactivators such as P300 that catalyze acetylation of histones.
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A Benchmark of Hemoglobin Blocking During Library Preparation for Mrna
www.nature.com/scientificreports OPEN A benchmark of hemoglobin blocking during library preparation for mRNA-Sequencing of human blood samples Florian Uellendahl-Werth1, Markus Wolfen2, Andre Franke1, Olaf Wolkenhauer2,3 & David Ellinghaus1* RNA-Sequencing (RNA-Seq) of peripheral blood can be a valuable source of information for investigating the status and mechanism of diseases. However, blood contains 50–80% unwanted hemoglobin (Hb) transcripts. Lexogen’s QuantSeq mRNA-Seq-Kit for Illumina RNA-Seq features a ‘Globin Block’ (GB) module that depletes Hb cDNAs during library preparation. Here, we aimed to assess GB’s efectiveness and checked for technical biases attributable to GB. Using whole blood total RNA samples of 91 healthy individuals, we sequenced 91 pairs of GB and non-blocked samples (noGB) on Illumina HiSeq2500 and 8 pairs of GB/noGB technical replicates on HiSeq4000. GB reduced the fraction of Hb transcripts from 43% (s.d. 14%) to 8.0% (s.d. 4.3%). From GB samples we detected 1,397 more expressed genes at approximately 11 million reads per RNA-isolate. Enrichment and diferential expression analyses did not reveal signifcant diferences for GB and noGB samples with respect to molecular function. In contrast to results from studies that have examined the performance of GB during RNA isolation, we were able to assign GB to corresponding noGB samples (from multiple sequencing runs on HiSeq2500) with at least 89.8% accuracy from the complete correlation matrix of all GB/GB, noGB/noGB and GB/noGB pairs. However, the use of diferent sequencers (HiSeq2500 vs HiSeq4000) impaired assignment of technical replicates, whereas assignment of GB to corresponding noGB samples worked perfectly when sequencing on one lane on HiSeq4000.
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