Supplementary Table 1. Primers for Q-RT-PCR

Gene Sequence Product lenght AR CTCACCAAGCTCCTGGACTC 247 AR CAGGCAGAAGACATCTGAAAG 247 ASF1B ACAGGAGTTCATCCGAGTGG 164 ASF1B CCATGTTGTTGTCCCAGTTG 164 CDCA3 CCAAGGAGGAAGCAAGACAG 295 CDCA3 GCTCGTCCTCCAGTTTTCAG 295 DTL AAGTCCGTCATCCCAGACAC 100 DTL TGTGCAGATTTTCCTCATGG 100 ERG TCTTGGACCAACAAGTAGCC 151 ERG GTCGGGATCCGTCATCTTG 151 ETV1 GTTGGGGCATTCAGAAAAAC 165 ETV1 TATCTGGAAAGGCCATGGAG 165 KIAA0101 CTGAAGAGGCAGGAAGCAGT 134 KIAA0101 CCAGGGTAAACAAGGAGACG 134 NUSAP1 AGTTTGTCTCGTCCCCTCAA 167 NUSAP1 CGTTTCTTCCGTTGCTCTTC 167 PSA GCAGCATTGAACCAGAGGAG 393 PSA AGAACTGGGGAGGCTTGAGT 393 TBP GAATATAATCCCAAGCGGTTTG 226 TBP ACTTCACATCACAGCTCCCC 226 UBE2T GTGGACAGAGAAGCATGCAA 208 UBE2T TCACCTTGGCAAAGAACACA 208 Supplementary Table 2. Androgen responsive genes in LNCaP-pcDNA3.1 according to microarray expression analysis Ratio values

Official symbol RefSeq Acc# 0nM DHT 1.0nM DHT 10nM DHT 100nM DHT 4h: > 2-fold up-regulated at 4 h FKBP5 NM_004117 1 1.197 1.803 2.229 KCNN2 NM_021614 1 1.307 2.324 2.586 C1orf116 NM_023938 1 1.332 2.467 2.528 TMPRSS2 NM_005656 1 1.632 4.844 6.09 SLC45A3 NM_033102 1 1.63 3.194 3.843 TMEPAI NM_020182 1 1.364 2.197 2.631 KLK2 NM_001002231 1 1.102 2.592 3.08 KCNN2 NM_021614 1 1.435 2.032 2.393 C19orf48 NM_199249 1 1.225 1.876 2.065 C19orf48 NM_199249 1 1.42 2.217 2.325 RTN4 NM_007008 1 1.586 2.054 2.372 NKX3-1 NM_006167 1 1.48 2.972 3.572 LOC400451 NM_207446 1 1.275 1.9 2.033 ZBTB16 NM_001018011 1 2.204 6.005 6.737 TMEPAI NM_020182 1 1.37 3.526 3.973 ZNF187 NM_001023560 1 2.239 2.733 2.288 FAM110B NM_147189 1 1.086 1.725 2.097 GHR NM_000163 1 1.513 1.932 2.02 MBOAT2 NM_138799 1 1.262 1.887 2.056 SGK NM_005627 1 0.961 1.529 2.041 IRS2 NM_003749 1 1.388 1.989 2.519 RHOU NM_021205 1 1.663 3.86 4.136 CLEC2D NM_001004419 1 1.411 2.105 1.745 KLK3 NM_001030047 1 0.966 2.071 2.973

>2-fold downregulated at 4h KCNJ13 NM_002242 1 0.602 0.491 0.537 LDOC1 NM_012317 1 0.505 0.395 0.493 CCDC83 NM_173556 1 0.541 0.485 0.464 C20orf46 NM_018354 1 0.443 0.447 0.488 MT2A NM_005953 1 0.481 0.469 0.567 MT1A NM_005946 1 0.418 0.402 0.517 LCN2 NM_005564 1 0.569 0.504 0.497 DUSP5 NM_004419 1 0.635 0.508 0.48 IGFBP3 NM_000598 1 0.415 0.465 0.491 EFHC2 NM_025184 1 0.491 0.449 0.503 ANXA1 NM_000700 1 0.422 0.357 0.4 SYT11 NM_152280 1 0.471 0.459 0.504 TNNT1 NM_003283 1 0.429 0.426 0.574 PLA2G2A NM_000300 1 0.359 0.341 0.432 MT1G NM_005950 1 0.271 0.261 0.355 BASP1 NM_006317 1 0.562 0.397 0.429 TLL1 NM_012464 1 0.448 0.415 0.536 CP NM_000096 1 0.473 0.425 0.475 TGM3 NM_003245 1 0.578 0.499 0.597 DAPK1 NM_004938 1 0.496 0.41 0.497 SDCBP2 NM_015685 1 0.518 0.493 0.547 SDCBP2 NM_015685 1 0.453 0.414 0.44 MT1X NM_005952 1 0.434 0.407 0.532 PIGR NM_002644 1 0.463 0.469 0.481 LOC440087 NM_001013698 1 0.55 0.5 0.528 S100P NM_005980 1 0.365 0.395 0.411 MT1F NM_005949 1 0.532 0.481 0.605 KLHL1 NM_020866 1 0.457 0.458 0.575 >2-fold upregulated at 24h SORD NM_003104 1 1.439 1.602 2.137 KLK3 NM_001030047 1 0.871 2.533 4.188 PTTG1 NM_004219 1 1.677 2.211 1.751 PSMC4 NM_006503 1 2.017 1.951 1.595 C21orf100 1 1.711 2.023 1.689 RLN1 NM_006911 1 1.67 2.083 2.74 UGT2B17 NM_001077 1 2.422 1.628 0.973 CDC20 NM_001255 1 1.212 2.028 2.336 KCNN2 NM_021614 1 1.207 2.192 3.59 ATP5F1 NM_001688 1 2.042 2.119 2.013 CDCA5 NM_080668 1 1.24 2.936 3.326 MAD2L1 NM_002358 1 1.238 1.984 2.598 TCP1 NM_001008897 1 2.406 2.478 2.326 DC2 NM_021227 1 1.79 1.964 2.16 CENPN NM_018455 1 1.231 2.045 3.903 TMPRSS2 NM_005656 1 0.813 1.358 2.847 DHCR24 NM_014762 1 0.889 1.433 2.243 CDC25A NM_001789 1 1.133 1.714 2.137 SLC45A3 NM_033102 1 0.992 1.509 2.223 PPAPDC1B NM_032483 1 1.624 2.243 2.301 HMBS NM_000190 1 1.837 2.109 2.138 SEMA6A NM_020796 1 2.153 1.744 1.232 FIGNL1 NM_001042762 1 1.584 2.079 1.93 DBI NM_020548 1 1.254 1.692 2.203 HIST1H2AC NM_003512 1 2.074 1.939 1.63 MCM2 NM_004526 1 0.919 1.721 2.074 MCM4 NM_005914 1 1.196 2.362 2.93 PIGW NM_178517 1 1.426 2.035 1.983 TMEPAI NM_020182 1 1.074 1.6 2.728 EHF NM_012153 1 1.961 1.998 2.101 TSC22D1 NM_006022 1 1.861 1.905 2.147 TMSL8 NM_021992 1 1.711 2.198 2.233 PAK1IP1 NM_017906 1 1.303 1.621 2.072 CAPRIN1 NM_005898 1 2.103 1.883 1.618 KLK2 NM_001002231 1 0.995 1.209 2.204 SLC39A6 NM_012319 1 1.739 2.03 2.017 DTL NM_016448 1 0.995 1.963 2.611 CDC2 NM_001786 1 1.904 3.259 2.652 C19orf48 NM_199249 1 0.708 1.237 2.108 LDLR NM_000527 1 1.306 1.645 2.16 CENPM NM_001002876 1 1.09 1.997 2.172 FEN1 NM_004111 1 1.034 1.985 2.133 UGT2B11 NM_001073 1 2.308 1.549 0.928 FBXL17 NM_022824 1 2.438 1.966 1.377 PDIA5 NM_006810 1 1.231 1.761 2.521 TRIP13 NM_004237 1 1.194 1.886 2.153 MCM5 NM_006739 1 1.014 1.659 2.037 NKX3-1 NM_006167 1 1.024 1.624 2.973 SRP9 NM_003133 1 2.345 2.018 1.867 FKBP11 NM_016594 1 1.137 1.634 2.144 CDCA7 NM_031942 1 1.024 1.917 2.658 EPB41L4B NM_018424 1 2.14 1.978 1.679 ID2 NM_002166 1 2.146 1.863 1.915 ACTG1 NM_001614 1 2.364 2.1 1.998 IDH1 NM_005896 1 1.945 2.216 2.343 KIF22 NM_007317 1 1.345 1.775 2.084 CCNB2 NM_004701 1 1.22 2.15 2.182 C1orf43 NM_015449 1 1.963 2.144 2.188 DSEL NM_032160 1 1.437 1.403 2.116 ZBTB16 NM_001018011 1 0.988 1.374 2 MCM7 NM_005916 1 1.085 1.704 2.154 TUBA1A NM_006009 1 2.009 2.196 1.834 NIPSNAP3A NM_015469 1 2.292 1.805 1.22 C7orf24 NM_024051 1 1.54 2.048 2.23 MB NM_005368 1 2.128 2.182 1.715 SLC4A4 NM_003759 1 1.729 2.443 2.795 LEPREL1 NM_018192 1 2.117 1.942 1.657 TMEPAI NM_020182 1 1.138 1.929 3.989 UHRF1 NM_013282 1 1.349 2.633 3.623 LYAR NM_017816 1 1.442 1.923 2.138 TYMS NM_001071 1 1.661 3.74 4.543 NUSAP1 NM_016359 1 1.318 2.7 2.691 PPAP2A NM_003711 1 1.554 2.043 2.198 ID1 NM_002165 1 2.141 1.954 1.656 IL8 NM_000584 1 2.083 2.081 1.8 PASK NM_015148 1 1.282 1.837 2.212 MANEA NM_024641 1 2.333 1.806 1.218 GINS2 NM_016095 1 1.044 2.131 2.798 TMEFF2 NM_016192 1 1.334 2 2.638 FGFR3 NM_000142 1 1.222 1.876 2.211 PCNA NM_002592 1 1.282 1.746 2.464 CCNE2 NM_057735 1 1.601 2.065 1.838 RPL29 NM_000992 1 1.399 1.878 2.178 STT3B NM_178862 1 2.07 2.007 2.071 ATAD2 NM_014109 1 1.577 3.009 3.023 CCT6A NM_001009186 1 2.185 2.319 2.049 CNIH NM_001009551 1 1.934 1.943 2.005 C1orf80 NM_022831 1 2.579 2.395 2.121 CLEC2D NM_001004419 1 1.739 1.306 2.95 TK1 NM_003258 1 1.182 2.315 2.394 SEC11C NM_033280 1 1.238 1.663 2.001 DEGS1 NM_003676 1 1.228 1.729 2.012 UBE2C NM_007019 1 1.334 2.792 2.623 GULP1 NM_016315 1 2.447 2.015 1.693 AADAT NM_016228 1 1.726 2.319 2.856 CACYBP NM_001007214 1 1.91 2.093 2.164 FBXO5 NM_012177 1 1.326 1.933 2.125 MRPL45 NM_032351 1 2.068 2.178 1.921 KLK3 NM_001030047 1 0.809 1.985 2.634 KIAA0101 NM_001029989 1 2.538 4.662 4.382

>2-fold downregulated at 24h LDOC1 NM_012317 1 0.494 0.481 0.475 TNNT1 NM_003283 1 0.444 0.403 0.428 PLA2G2A NM_000300 1 0.241 0.178 0.163 C4orf18 NM_001031700 1 0.763 0.584 0.379 C4orf18 NM_001031700 1 0.998 0.726 0.485 ATP1B1 NM_001001787 1 0.888 0.851 0.467 TLL1 NM_012464 1 0.899 0.682 0.4 PBEF1 NM_005746 1 1.034 0.684 0.487 TGM3 NM_003245 1 0.388 0.355 0.285 SLC30A4 NM_013309 1 0.302 0.244 0.188 OAZ2 NM_002537 1 0.615 0.577 0.498 TFF3 NM_003226 1 0.522 0.493 0.496 LGALS3BP NM_005567 1 0.494 0.462 0.386 S100P NM_005980 1 0.301 0.315 0.327 DHRS3 NM_004753 1 0.439 0.421 0.445 COL5A2 NM_000393 1 0.656 0.554 0.398 ACPP NM_001099 1 0.71 0.584 0.499 RARRES3 NM_004585 1 0.771 0.553 0.48 Supplementary Table 3. Androgen responsive genes in LNCaP-ARmo according to microarray expression analysis Ratio values

Official symbol RefSeq Acc# 0nM DHT 1.0nM DHT 10nM DHT 100nM DHT 4h: > 2-fold up-regulated at 4 h SORD NM_003104 1 2.169 2.229 2.254 KLK3 NM_001030047 1 1.778 2.464 2.744 UBE2G1 NM_003342 1 1.693 1.958 2.307 FKBP5 NM_004117 1 4.156 6.805 8.16 LRRFIP2 NM_006309 1 1.683 2.149 2.628 ELL2 NM_012081 1 2.104 2.602 3.235 NDRG1 NM_006096 1 1.181 1.649 2.146 KCNN2 NM_021614 1 1.256 1.655 2.271 CDCA5 NM_080668 1 2.075 1.891 1.442 SIX5 NM_175875 1 2.39 2.319 2.195 C1orf21 NM_030806 1 1.316 1.762 2.001 SPRED1 NM_152594 1 1.728 2.106 2.427 C1orf116 NM_023938 1 2.582 3.106 3.717 KLF6 NM_001008490 1 1.582 2.148 1.992 CENPN NM_018455 1 2.2 2.751 3.434 KIF20A NM_005733 1 2.188 2.115 1.604 IFI44 NM_006417 1 2.466 1.892 1.642 TMPRSS2 NM_005656 1 5.722 8.888 12.51 GUCY1A3 NM_000856 1 1.709 1.871 2.147 RAB4A NM_004578 1 1.607 1.788 2.303 SLC45A3 NM_033102 1 4.086 5.227 6.225 ISG15 NM_005101 1 2.186 2.077 1.629 KBTBD11 NM_014867 1 1.821 2.152 2.196 RASD1 NM_016084 1 1.751 3.843 4.472 C17orf48 NM_020233 1 1.375 1.85 2.243 CAMKK2 NM_006549 1 1.151 1.712 2.176 MPHOSPH9 NM_022782 1 2.091 2.555 2.59 NEDD4L NM_015277 1 1.992 2.338 2.67 TMEPAI NM_020182 1 1.882 2.821 3.034 KLK4 NM_004917 1 2.309 2.63 2.938 TBC1D8 NM_007063 1 1.382 1.767 2.215 GRHL2 NM_024915 1 1.596 2.372 2.989 HERC5 NM_016323 1 2.169 2.109 1.812 ACSL3 NM_004457 1 1.697 1.85 2.419 EHF NM_012153 1 1.75 1.716 2.018 MAP7D1 NM_018067 1 1.727 2.033 2.05 TSC22D1 NM_006022 1 1.714 1.899 2.436 DNM1L NM_005690 1 1.665 1.908 2.089 PAK1IP1 NM_017906 1 1.363 1.651 2.067 FAM13A1 NM_001015045 1 1.583 1.97 2.572 KRT8 NM_002273 1 1.451 1.735 2.104 ERRFI1 NM_018948 1 2.28 3.1 4.748 CEBPD NM_005195 1 1.48 1.548 2.056 ADD3 NM_001121 1 2.335 1.809 1.898 ADD3 NM_001121 1 2.344 2.246 2.094 CDC2 NM_001786 1 2.211 2.052 1.573 C19orf48 NM_199249 1 2.689 2.985 3.144 TOP2A NM_001067 1 2.225 1.881 1.528 TIPARP NM_015508 1 3.085 5.011 7.584 SHROOM3 NM_020859 1 1.552 1.597 2.13 EAF2 NM_018456 1 1.355 1.894 2.436 HERC3 NM_014606 1 1.644 1.915 2.003 NKX3-1 NM_006167 1 2.54 2.949 3.777 ATAD4 NM_024320 1 1.989 2.461 1.485 CXCL10 NM_001565 1 2.541 1.944 1.618 MTMR9 NM_015458 1 1.625 2.03 2.4 RDH10 NM_172037 1 2.264 3.043 4.134 EPB41L4B NM_018424 1 2.056 2.154 2.291 HERC6 NM_017912 1 1.81 2.019 1.918 KIF22 NM_007317 1 1.934 2.624 2.516 FAM105A NM_019018 1 1.892 3.265 4.25 CCNB2 NM_004701 1 2.047 1.82 1.454 GADD45G NM_006705 1 1.568 1.928 2.575 ZBTB16 NM_001018011 1 7.072 8.563 9.035 IFIT1 NM_001001887 1 3.41 2.562 1.984 FSTL1 NM_007085 1 2.009 2.202 2.121 CROT NM_021151 1 2.39 3.157 2.874 OASL NM_003733 1 3.784 2.701 2.206 SLC4A4 NM_003759 1 1.88 1.833 2.508 LRRFIP2 NM_006309 1 1.788 2.041 2.452 TMEPAI NM_020182 1 4.135 5.407 6.21 PPM1K NM_152542 1 1.997 2.044 2.329 TYMS NM_001071 1 2.181 1.968 1.584 BBX NM_020235 1 1.942 2.56 3.053 PPAP2A NM_003711 1 1.664 1.99 2.357 FAM110B NM_147189 1 1.355 1.928 2.192 NCAPD3 NM_015261 1 2.595 4.084 5.21 ALDH1A3 NM_000693 1 1.334 1.793 2.343 ERN1 NM_001433 1 1.668 2.259 2.66 GHR NM_000163 1 2.694 2.417 2.467 SGK NM_005627 1 4.695 6.793 9.403 LONRF1 NM_152271 1 2.27 2.414 2.841 SLC36A1 NM_078483 1 1.602 2.097 2.421 ABCC4 NM_005845 1 2.17 2.65 3.452 GCNT1 NM_001490 1 1.505 1.805 2.275 PPAP2A NM_003711 1 1.816 2.016 2.141 PPAP2A NM_003711 1 1.49 1.926 2.122 SLC2A12 NM_145176 1 1.944 2.147 2.396 IRS2 NM_003749 1 1.431 1.962 2.718 NFIB NM_005596 1 1.47 1.743 2.043 IFIT2 NM_001547 1 4.102 2.95 2.194 ATAD2 NM_014109 1 2.843 2.785 3.188 LRIG1 NM_015541 1 2.013 2.897 3.647 MARCH3 NM_178450 1 1.372 1.668 2.092 RHOU NM_021205 1 5.602 7.499 9.193 DRB1 NM_152945 1 1.321 1.781 2.152 HIST1H3H NM_003536 1 2.1 1.948 1.957 F2RL1 NM_005242 1 1.789 2.116 2.063 CLEC2D NM_001004419 1 1.802 1.377 2.078 TK1 NM_003258 1 2.082 1.907 1.373 APRIN 1 1.658 1.737 2.083 ZNF533 NM_152520 1 1.865 2.045 2.308 SLC44A3 NM_152369 1 2.011 1.979 1.979 KCTD3 NM_016121 1 1.535 1.961 2.585 BAPX1 NM_001189 1 1.586 1.867 2.051 HMGB2 NM_002129 1 2.205 1.896 1.793 SASH1 NM_015278 1 1.79 2.274 2.319 KLK3 NM_001030047 1 3.109 4.337 4.981 KIAA0101 NM_001029989 1 3.454 3.026 2.235 PTGFR NM_000959 1 2.242 2.143 2.117 RARRES3 NM_004585 1 3.073 2.609 2.16

>2-fold downregulated at 4h C21orf100 1 0.288 0.277 0.303 LDOC1 NM_012317 1 0.452 0.403 0.495 OBFC2A 1 0.524 0.492 0.558 MT2A NM_005953 1 0.47 0.496 0.638 LMO4 NM_006769 1 0.537 0.483 0.425 MT1A NM_005946 1 0.432 0.455 0.534 UGT2B28 NM_053039 1 0.549 0.449 0.44 GDF15 NM_004864 1 0.495 0.527 0.691 FLJ20054 NM_019049 1 0.579 0.498 0.46 SI NM_001041 1 0.516 0.454 0.504 HMOX1 NM_002133 1 0.403 0.347 0.269 EFHC2 NM_025184 1 0.372 0.394 0.361 ZBTB43 NM_014007 1 0.427 0.402 0.424 SLITRK3 NM_014926 1 0.392 0.324 0.341 OSR2 NM_053001 1 0.51 0.44 0.395 ENC1 NM_003633 1 0.433 0.405 0.42 BIRC3 NM_001165 1 0.459 0.39 0.422 NFKB1 NM_003998 1 0.568 0.461 0.46 PEG3 NM_006210 1 0.225 0.201 0.192 BARD1 NM_000465 1 0.575 0.513 0.479 KRT80 NM_182507 1 0.5 0.528 0.483 ELF3 NM_004433 1 0.567 0.514 0.476 TNNT1 NM_003283 1 0.439 0.465 0.433 KCNS3 NM_002252 1 0.525 0.502 0.478 PLA2G2A NM_000300 1 0.351 0.383 0.634 TNFRSF12A NM_016639 1 0.453 0.484 0.452 MT1G NM_005950 1 0.335 0.349 0.513 OR51E1 NM_152430 1 0.454 0.481 0.439 EFNB2 NM_004093 1 0.363 0.318 0.39 ABCA1 NM_005502 1 0.369 0.381 0.348 TLL1 NM_012464 1 0.443 0.417 0.443 NAALAD2 NM_005467 1 0.539 0.49 0.56 SOX8 NM_014587 1 0.613 0.547 0.48 C5orf30 NM_033211 1 0.429 0.381 0.374 CCL20 NM_004591 1 0.397 0.362 0.374 TGM3 NM_003245 1 0.421 0.472 0.536 SLC30A4 NM_013309 1 0.773 0.327 0.335 LOC138046 NM_173848 1 0.471 0.498 0.455 SDCBP2 NM_015685 1 0.486 0.509 0.53 MT1X NM_005952 1 0.397 0.444 0.547 TFF3 NM_003226 1 0.363 0.428 0.537 FLJ23049 NM_024687 1 0.413 0.433 0.395 UGT2B10 NM_001075 1 0.531 0.465 0.425 ZNF294 NM_015565 1 0.694 0.527 0.482 OPRK1 NM_000912 1 0.412 0.334 0.372 ST7 NM_018412 1 0.389 0.397 0.436 PTPRR NM_002849 1 0.415 0.41 0.423 CBLN2 NM_182511 1 0.531 0.398 0.386 MT1F NM_005949 1 0.383 0.418 0.476 ANKRD16 NM_001009941 1 0.493 0.421 0.418 BEST1 NM_004183 1 0.368 0.4 0.447 BCHE NM_000055 1 0.275 0.265 0.312 KLHL1 NM_020866 1 0.321 0.197 0.282

>2-fold upregulated at 24h SORD NM_003104 1 2.365 4.445 7.727 ORM2 NM_000608 1 1.016 0.951 3.224 TSC2 NM_000548 1 1.311 1.799 2.439 KLK3 NM_001030047 1 2.671 4.493 6.046 UBE2G1 NM_003342 1 1.368 1.701 2.582 MICAL1 NM_022765 1 1.23 2.14 6.922 DUSP2 NM_004418 1 1.085 1.554 2.117 FKBP5 NM_004117 1 1.264 2.994 14.49 ELOVL5 NM_021814 1 1.719 2.276 2.834 ELL2 NM_012081 1 1.507 2.224 4.159 PDIA6 NM_005742 1 1.504 1.808 2.014 RAG1AP1 NM_018845 1 1.122 1.463 2.016 FOXD4L1 NM_012184 1 1.337 1.519 2.662 NDRG1 NM_006096 1 0.47 0.907 4.705 PEX10 NM_002617 1 1.281 1.874 3.404 USP10 NM_005153 1 1.255 1.378 2.272 UFM1 NM_016617 1 1.515 1.77 2.031 RAP1GAP NM_002885 1 0.708 0.995 2.212 RLN1 NM_006911 1 2.175 2.822 3.058 ABCC1 NM_004996 1 1.054 1.26 2.428 SLC16A6 NM_004694 1 1.016 0.951 3.718 GSR NM_000637 1 1.399 1.787 2.074 KCNN2 NM_021614 1 1.411 2.565 2.805 TUBA3D NM_080386 1 1.026 1.379 3.548 INSIG1 NM_005542 1 1.46 2.01 3.155 CDCA5 NM_080668 1 1.31 2.172 1.543 CREB3L2 NM_194071 1 0.957 1.314 2.087 C16orf61 NM_020188 1 1.233 1.681 2.064 C1orf21 NM_030806 1 1.049 1.414 2.229 MAD2L1 NM_002358 1 1.523 2.24 1.585 TCP1 NM_001008897 1 2.293 2.357 2.208 WIPI1 NM_017983 1 1.016 1.186 3.553 TUBA3E NM_207312 1 1.016 0.951 5.827 UGT2B28 NM_053039 1 0.475 0.87 2.361 C1orf116 NM_023938 1 1.297 1.632 2.383 PSMA6 NM_002791 1 1.43 1.739 2.709 CREB3L4 NM_130898 1 1.281 1.47 2.547 DNM1L NM_005690 1 1.441 1.664 2.6 GMPPB NM_013334 1 1.071 1.732 2.982 DC2 NM_021227 1 1.848 2.499 3.143 CENPN NM_018455 1 2.066 5.065 9.963 CCNC NM_001013399 1 1.898 2.073 1.994 IFI44 NM_006417 1 2.426 2.582 1.853 TMPRSS2 NM_005656 1 1.491 2.704 6.188 TRPM4 NM_017636 1 1.006 1.464 2.268 GUCY1A3 NM_000856 1 1.446 1.932 2.203 MALT1 NM_006785 1 1.488 1.753 2.857 ANKRD37 NM_181726 1 0.941 1.286 4.325 SLC35F2 NM_017515 1 1.409 1.898 3.52 C9orf152 NM_001012993 1 1.152 1.378 2.6 DHCR24 NM_014762 1 1.622 2.295 3.777 CLDN8 NM_199328 1 1.227 1.417 4.141 RAB4A NM_004578 1 1.478 1.629 2.247 CDC25A NM_001789 1 1.187 1.756 2.168 HERPUD1 NM_001010989 1 1.24 1.737 2.781 SLC45A3 NM_033102 1 1.659 2.543 4.714 C4orf34 NM_174921 1 1.212 1.701 2.195 AMACR NM_014324 1 2.066 2.963 4.44 ABHD3 NM_138340 1 1.477 1.714 2.363 PPAPDC1B NM_032483 1 2.271 3.382 3.894 IGFBP3 NM_000598 1 2.971 2.584 2.184 HMBS NM_000190 1 1.832 2.319 2.101 C6orf51 NM_138408 1 1.293 1.268 2.292 DBI NM_020548 1 1.748 2.618 3.992 SH3BGRL NM_003022 1 1.689 1.869 2.31 C14orf24 NM_173607 1 1.294 1.494 2.053 C17orf48 NM_020233 1 1.233 1.391 2.16 TMED9 NM_017510 1 1.092 1.427 2.132 HIST1H2AC NM_003512 1 2.077 1.966 1.683 MPHOSPH9 NM_022782 1 1.184 1.755 2.936 DERL2 NM_016041 1 1.185 1.437 2.109 AZGP1 NM_001185 1 1.984 2.803 3.234 AR NM_000044 1 1.544 1.62 2.028 PIGW NM_178517 1 1.592 2.114 1.878 PHACTR2 NM_014721 1 1.119 1.358 2.321 TMEPAI NM_020182 1 1.498 2.825 4.643 TBC1D8 NM_007063 1 1.016 0.962 2.098 WWC1 NM_015238 1 1.093 1.31 2.434 SEC61B NM_006808 1 1.152 1.473 2.062 BCAP29 NM_001008405 1 2.086 2.083 2.805 GRHL2 NM_024915 1 1.179 1.343 2.155 ACSL3 NM_004457 1 1.831 2.55 4.288 ACSL3 NM_004457 1 1.016 0.951 2.085 EHF NM_012153 1 1.849 1.959 2.032 TMED7 NM_181836 1 1.502 1.548 2.111 GOSR2 NM_001012511 1 1.198 1.503 2.017 PEA15 NM_003768 1 1.215 1.552 2.163 BRP44 NM_015415 1 1.53 1.889 2.424 DNASE2B NM_021233 1 1.683 2.04 2.879 TSC22D1 NM_006022 1 1.777 1.987 4.018 DNM1L NM_005690 1 1.276 1.767 2.371 TPM1 NM_000366 1 1.235 1.543 2.261 PAK1IP1 NM_017906 1 1.474 1.919 5.227 KLK2 NM_001002231 1 1.016 1.817 3.566 KRT8 NM_002273 1 0.721 1.251 3.442 SLC33A1 NM_004733 1 1.795 1.745 2.617 SLC39A6 NM_012319 1 1.854 2.138 1.796 DTL NM_016448 1 1.516 2.439 1.601 SLC2A3 NM_006931 1 1.016 0.951 2.717 EFCAB4A NM_173584 1 1.387 1.695 2.15 GLRX2 NM_016066 1 1.164 1.638 2.655 ERRFI1 NM_018948 1 1.018 1.195 5.34 MPZL1 NM_003953 1 1.399 1.776 2.661 CDC2 NM_001786 1 1.374 2.004 1.711 PPFIBP2 NM_003621 1 1.038 1.542 2.218 SC4MOL NM_001017369 1 1.198 1.253 2.121 C19orf48 NM_199249 1 1.143 1.723 2.078 TIPARP NM_015508 1 1.016 0.951 4.533 CADPS2 NM_001009571 1 1.103 1.307 2.707 LDLR NM_000527 1 1.661 2.743 5.9 LPXN NM_004811 1 1.354 1.981 2.241 SEC24D NM_014822 1 1.016 1.172 2.615 PRRT2 NM_145239 1 0.928 1.026 2.078 TSPAN13 NM_014399 1 1.421 1.884 2.246 KDELR2 NM_006854 1 1.397 2.073 2.678 UAP1 NM_003115 1 1.221 1.757 3.144 FEN1 NM_004111 1 1.426 2.096 1.956 ORM1 NM_000607 1 1.016 1.055 3.596 PDIA5 NM_006810 1 1.797 2.509 3.71 EAF2 NM_018456 1 1.016 1.735 4.348 HERC3 NM_014606 1 1.406 2.352 3.554 FOXD4 NM_207305 1 1.016 1.131 2.294 RNASE4 NM_002937 1 1.02 1.276 2.025 MLPH NM_001042467 1 1.155 1.476 2.413 LYPLAL1 NM_138794 1 1.639 2.061 2.392 C17orf79 NM_018405 1 1.093 1.486 2.045 NKX3-1 NM_006167 1 1.364 2.052 3.185 FKBP11 NM_016594 1 1.982 2.912 3.692 CDCA7 NM_031942 1 1.601 2.615 1.686 MTMR9 NM_015458 1 1.101 1.389 2.46 KRT18 NM_000224 1 1.27 2.02 4.019 SERP1 NM_014445 1 1.479 1.518 2.557 RDH10 NM_172037 1 1.331 1.18 3.037 FAM105B NM_138348 1 1.147 1.313 2.136 EPB41L4B NM_018424 1 1.903 1.89 2.093 TPD52L1 NM_001003395 1 1.579 2.029 1.856 ID2 NM_002166 1 1.416 1.622 2.903 RAB3B NM_002867 1 1.089 1.637 2.337 ACACA 1 1.491 1.928 2.152 UCHL3 NM_006002 1 1.286 1.561 2.114 GPR89B NM_016334 1 1.571 1.745 2.126 IDH1 NM_005896 1 2.001 2.313 2.367 MGC14376 NM_001001870 1 1.393 1.769 3.518 KIF22 NM_007317 1 1.387 2.222 4.008 SLC43A1 NM_003627 1 0.937 1.327 2.153 FAM105A NM_019018 1 1.016 1.247 5.561 GADD45G NM_006705 1 0.869 0.927 4.26 PTPRM NM_002845 1 1.53 1.893 3.299 NANS NM_018946 1 1.244 1.685 2.472 FADS1 NM_013402 1 1.345 1.786 3.707 ST6GALNAC1 NM_018414 1 1.016 2.135 3.624 LAMC1 NM_002293 1 1.189 1.916 2.83 AMACR NM_014324 1 1.648 1.82 2.772 STK39 NM_013233 1 1.415 1.89 3.186 LOC400451 NM_207446 1 1.22 1.742 2.167 SELS NM_018445 1 1.151 1.557 2.415 GLYATL2 NM_145016 1 1.23 1.85 3.21 ZBTB16 NM_001018011 1 1.296 1.843 3.087 ECD NM_007265 1 1.692 1.794 2 SPRY1 NM_005841 1 1.822 2.142 1.624 RNASE4 NM_002937 1 0.99 1.349 2.082 EDG7 NM_012152 1 1.462 1.948 3.862 SMS NM_004595 1 1.115 2.028 3.777 TUBA1A NM_006009 1 1.467 1.813 2.018 GMPPA NM_013335 1 1.011 1.454 2.394 AGR2 NM_006408 1 1.318 2.228 4.26 HM13 NM_030789 1 1.495 2.071 2.563 GLRX2 NM_016066 1 1.327 1.823 2.07 B2M NM_004048 1 1.623 1.867 2.652 C7orf24 NM_024051 1 1.738 2.23 2.028 MERTK NM_006343 1 1.016 0.951 2.247 FAM18B NM_016078 1 1.695 1.974 2.003 DNASE2B NM_021233 1 1.234 1.672 2.557 KCNMA1 NM_001014797 1 1.215 1.428 2.354 MB NM_005368 1 2.13 2.317 1.484 SLC4A4 NM_003759 1 2.017 2.342 2.648 FAT NM_005245 1 1.683 2.085 2.572 LRRFIP2 NM_006309 1 1.046 1.238 2.334 TMEPAI NM_020182 1 2.086 4.338 6.969 UHRF1 NM_013282 1 1.21 2.034 1.479 DNAJB9 NM_012328 1 1.229 1.724 3.805 LYAR NM_017816 1 1.523 2.009 1.597 ARF4 NM_001660 1 2.03 2.591 3.312 LCP1 NM_002298 1 1.721 2.297 2.595 MTP18 NM_001003704 1 0.994 1.597 2.566 SRP19 NM_003135 1 1.214 1.355 2.108 PPAP2A NM_003711 1 1.846 2.799 5.271 ID1 NM_002165 1 1.351 1.62 2.597 KRT18 NM_000224 1 1.686 2.351 4.238 CYP2U1 NM_183075 1 1.016 0.951 2.036 ABHD2 NM_007011 1 1.46 2.073 3.953 SPRYD5 NM_032681 1 0.873 1.587 4.369 PECI NM_006117 1 1.211 1.314 2.159 ELOVL1 NM_022821 1 1.497 1.815 2.357 NCAPD3 NM_015261 1 1.187 2.743 16.25 ALDH1A3 NM_000693 1 1.544 2.357 4.056 ACOT9 NM_001033583 1 1.439 1.779 2.047 HMGCS1 NM_002130 1 1.2 1.39 2.387 PASK NM_015148 1 1.443 2.417 3.336 ERN1 NM_001433 1 1.102 1.316 2.973 FRAP1 NM_004958 1 1.085 1.307 2.029 FASN NM_004104 1 1.341 1.877 3.334 SLC41A1 NM_173854 1 0.989 1.078 2.972 ODC1 NM_002539 1 1.012 1.566 4.073 TSC22D1 NM_006022 1 0.969 1.098 2.044 MBOAT2 NM_138799 1 1.294 1.873 3.36 ITGAV NM_002210 1 1.767 1.862 3.088 SGK NM_005627 1 1.016 0.951 5.781 PGC NM_002630 1 1.026 1.798 3.003 LONRF1 NM_152271 1 1.232 1.365 2.696 PRKCA NM_002737 1 0.734 1.113 2.712 HOMER2 NM_004839 1 1.143 1.979 3.223 ABCC4 NM_005845 1 1.552 2.286 3.47 TRIM48 NM_024114 1 1.016 1.205 3.649 FGFR3 NM_000142 1 1.252 2.027 2.535 SEPP1 NM_005410 1 1.475 1.796 2.755 ATP1A1 NM_000701 1 1.154 1.329 2.262 PPAP2A NM_003711 1 1.606 1.95 3.794 PPAP2A NM_003711 1 1.302 1.788 3.297 ARRDC1 NM_152285 1 1.073 1.299 2.219 ARMET NM_006010 1 1.034 1.432 2.104 IFIT2 NM_001547 1 2.173 2.365 1.77 GFPT1 NM_002056 1 1.171 1.454 2.003 LOC89944 NM_138342 1 1.166 1.551 3.113 STT3B NM_178862 1 1.829 1.851 2.127 BMPR1A NM_004329 1 1.148 1.428 2.496 ATAD2 NM_014109 1 1.761 2.919 5 LRIG1 NM_015541 1 1.195 1.308 3.011 MCEE NM_032601 1 1.361 1.683 2.041 DEFB32 NM_207469 1 1.016 1.713 2.622 UGDH NM_003359 1 1.111 1.438 2.807 SCD NM_005063 1 1.287 1.95 2.057 SAT1 NM_002970 1 1.046 1.436 2.163 GLUD1 NM_005271 1 0.983 1.377 2.141 TMEM79 NM_032323 1 1.251 2.676 4.066 ZBTB24 NM_014797 1 1.013 1.22 2.464 RHOU NM_021205 1 1.148 1.785 5.048 PGM3 NM_015599 1 1.522 1.947 3.484 HIST1H3H NM_003536 1 2.016 1.73 1.322 CLEC2D NM_001004419 1 2.305 2.441 1.487 SEC11C NM_033280 1 1.839 2.225 2.703 PCOTH NM_001014442 1 0.854 1.056 2.009 SLC31A2 NM_001860 1 1.112 1.242 2.013 ZNF533 NM_152520 1 1.546 1.657 2.415 KCTD3 NM_016121 1 1.16 1.152 2.066 SELS NM_018445 1 1.124 1.416 2.062 AADAT NM_016228 1 1.984 3.105 3.57 IDI1 NM_004508 1 1.247 1.257 2.174 SRPRB NM_021203 1 1.374 1.767 2.159 C1orf85 NM_144580 1 1.152 1.574 2.175 HYPE NM_007076 1 1.24 1.998 4.022 SMPD2 NM_003080 1 1.076 1.434 2.596 CHPT1 NM_020244 1 1.171 1.527 2.121 LIN7B NM_022165 1 1.115 1.905 3.922 CRELD2 NM_024324 1 1.159 1.565 2.156 CACYBP NM_001007214 1 1.784 2.016 1.585 SASH1 NM_015278 1 1.215 1.485 2.723 KLK3 NM_001030047 1 1.662 2.688 4.459 KIAA0101 NM_001029989 1 1.541 2.435 1.801 PTGFR NM_000959 1 1.75 1.662 2.047 HEBP2 NM_014320 1 1.334 1.419 2.125 SSR2 NM_003145 1 1.38 2.022 2.822

>2-fold downregulated at 24h EPHX1 NM_000120 1 0.638 0.518 0.386 CXCR7 NM_001047841 1 0.85 0.499 0.224 NUP93 NM_014669 1 0.719 0.728 0.495 LDOC1 NM_012317 1 0.65 0.513 0.362 SALL2 NM_005407 1 0.79 0.645 0.441 MMP7 NM_002423 1 0.655 0.425 0.336 C5orf13 NM_004772 1 0.711 0.59 0.442 SLC44A1 NM_080546 1 0.758 0.528 0.312 UGT2B17 NM_001077 1 0.69 0.344 0.129 UGT2B17 NM_001077 1 0.589 0.289 0.118 TRIB1 NM_025195 1 0.748 0.627 0.385 NLGN1 NM_014932 1 0.831 0.583 0.437 LUZP2 NM_001009909 1 0.864 0.599 0.438 TMEM144 NM_018342 1 1.078 0.8 0.487 FAM134B NM_001034850 1 0.954 0.692 0.39 NET1 NM_001047160 1 0.794 0.61 0.492 TCFL5 NM_006602 1 0.85 0.737 0.486 CERK NM_022766 1 0.864 0.596 0.467 FLJ20054 NM_019049 1 0.554 0.46 0.297 ENPP4 NM_014936 1 0.828 0.689 0.496 PHLDB1 NM_015157 1 0.735 0.711 0.484 TULP4 NM_001007466 1 0.762 0.589 0.357 MYT1 NM_004535 1 0.819 0.742 0.456 TNFRSF21 NM_014452 1 1.387 0.901 0.42 EPHA3 NM_005233 1 0.792 0.605 0.418 SYTL2 NM_032379 1 1.097 0.606 0.388 FKBP1A NM_000801 1 0.78 0.509 0.448 SMARCD3 NM_001003801 1 0.733 0.549 0.414 DDC NM_000790 1 0.903 0.601 0.294 C7orf41 NM_152793 1 0.677 0.508 0.479 HES6 NM_018645 1 0.608 0.516 0.422 TP53INP1 NM_033285 1 0.856 0.658 0.428 GRB10 NM_001001549 1 0.905 0.609 0.246 ZBTB43 NM_014007 1 0.84 0.635 0.435 SLITRK3 NM_014926 1 0.569 0.385 0.177 LPHN1 NM_001008701 1 0.713 0.682 0.5 OSR2 NM_053001 1 0.913 0.639 0.468 BIRC3 NM_001165 1 1.018 0.724 0.456 C9orf45 1 0.601 0.516 0.395 PEG3 NM_006210 1 0.463 0.674 0.442 CAMK2N1 NM_018584 1 0.798 0.506 0.221 LASP1 NM_006148 1 0.619 0.514 0.453 BARD1 NM_000465 1 0.812 0.725 0.352 SERPINI1 NM_005025 1 0.709 0.49 0.42 S100A13 NM_001024210 1 0.593 0.533 0.478 ALDH3B2 NM_000695 1 0.681 0.587 0.42 CYBASC3 NM_153611 1 1.031 0.705 0.491 ARHGEF2 NM_004723 1 0.615 0.583 0.429 FBXO2 NM_012168 1 0.583 0.422 0.393 TDP1 NM_001008744 1 0.662 0.597 0.322 INSIG2 NM_016133 1 0.811 0.627 0.481 H3F3B NM_005324 1 0.618 0.588 0.455 COBL NM_015198 1 0.746 0.534 0.46 PLA2G2A NM_000300 1 0.242 0.17 0.123 STRBP NM_018387 1 0.808 0.611 0.451 C4orf18 NM_001031700 1 0.824 0.509 0.285 C4orf18 NM_001031700 1 0.979 0.647 0.248 ST6GALNAC6 NM_013443 1 0.624 0.571 0.47 UGT2B7 NM_001074 1 0.539 0.402 0.623 ENO2 NM_001975 1 0.601 0.41 0.316 GALK1 NM_000154 1 0.706 0.666 0.453 NIPSNAP3A NM_015469 1 0.888 0.54 0.345 TP53INP2 NM_021202 1 0.609 0.49 0.697 TLL1 NM_012464 1 0.644 0.476 0.501 ST7 NM_018412 1 0.833 0.608 0.254 ST7 NM_018412 1 0.812 0.523 0.227 NAALAD2 NM_005467 1 0.593 0.491 0.465 C21orf81 NM_153750 1 0.848 0.603 0.428 C5orf30 NM_033211 1 0.807 0.666 0.463 TIMP2 NM_003255 1 0.722 0.478 0.391 CCL20 NM_004591 1 3.188 2.142 0.615 CTBP1 NM_001012614 1 0.855 0.671 0.3 CPNE3 NM_003909 1 0.882 0.68 0.496 COLEC12 NM_030781 1 1.212 0.875 0.407 FSCN1 NM_003088 1 0.548 0.506 0.376 TGM3 NM_003245 1 0.717 0.365 0.264 MANEA NM_024641 1 1.206 0.806 0.397 SLC30A4 NM_013309 1 0.224 0.176 0.263 LRRN1 NM_020873 1 1.403 0.997 0.411 FZD3 NM_017412 1 1.03 0.88 0.48 SDCBP2 NM_015685 1 1.407 0.783 0.319 OAZ2 NM_002537 1 0.586 0.545 0.484 TFF3 NM_003226 1 0.384 0.306 0.257 UGT2B10 NM_001075 1 0.487 0.456 0.617 ZNF294 NM_015565 1 1.152 0.823 0.359 NCOA7 NM_181782 1 1.058 0.744 0.375 OPRK1 NM_000912 1 0.893 0.535 0.181 KCTD17 NM_024681 1 0.612 0.564 0.498 ST7 NM_018412 1 0.731 0.428 0.195 DDEF2 NM_003887 1 0.921 0.676 0.436 SDC4 NM_002999 1 0.873 0.756 0.429 CPLX1 NM_006651 1 0.65 0.48 0.388 TMEM140 NM_018295 1 1.108 1.132 0.481 PLEKHB1 NM_021200 1 0.676 0.556 0.497 POLR3GL NM_032305 1 0.85 0.718 0.494 IL1RN NM_000577 1 0.544 0.368 0.303 MANBA NM_005908 1 0.811 0.65 0.454 CBLN2 NM_182511 1 0.749 0.676 0.341 SBK1 NM_001024401 1 0.63 0.603 0.48 ZIC2 NM_007129 1 1.687 1.065 0.474 KIAA0247 NM_014734 1 0.934 0.652 0.465 ABCG1 NM_004915 1 0.683 0.489 0.404 MT1F NM_005949 1 0.638 0.544 0.486 ANKRD16 NM_001009941 1 0.758 0.569 0.415 TMEM59L NM_012109 1 0.71 0.588 0.464 C1orf80 NM_022831 1 1.984 2.014 1.084 NUDT8 NM_181843 1 0.526 0.513 0.313 PFKP NM_002627 1 0.519 0.552 0.475 SLC44A1 NM_080546 1 0.945 0.725 0.449 BCHE NM_000055 1 0.444 0.393 0.414 PKIB NM_032471 1 1.112 0.967 0.414 PIK3IP1 NM_052880 1 0.949 0.851 0.485 PRKD1 NM_002742 1 0.887 0.762 0.473 FLJ11259 NM_018370 1 0.769 0.651 0.495 KLHL1 NM_020866 1 0.693 0.611 0.486 STK3 NM_006281 1 0.766 0.503 0.392 ACPP NM_001099 1 0.564 0.443 0.291 Supplementary Table 4. Androgen responsive genes in LNCaP-ARhi according to microarray expression analysis Ratio values

Official symbol RefSeq Acc# 0nM DHT 1.0nM DHT 10nM DHT 100nM DHT 4h: > 2-fold up-regulated at 4 h SSX2IP NM_014021 1 1.237 2.573 2.401 KLK3 NM_001030047 1 1.744 2.443 2.231 UBE2G1 NM_003342 1 2.064 2.602 2.687 DUSP2 NM_004418 1 1.709 2.354 2.658 FKBP5 NM_004117 1 3.277 8.642 9.754 RHOB NM_004040 1 1.225 2.995 3.077 LRRFIP2 NM_006309 1 1.973 3.238 3.189 ELL2 NM_012081 1 3.153 5.231 4.962 NDRG1 NM_006096 1 1.589 5.414 6.049 PEX10 NM_002617 1 1.918 2.142 2.093 LIFR NM_002310 1 1.058 1.984 2.043 SLC16A6 NM_004694 1 1.605 3.111 3.13 UGT2B17 NM_001077 1 2.547 2.077 2.177 KCNN2 NM_021614 1 4.031 6.393 6.324 MAD2L1 NM_002358 1 1.982 1.756 2.09 TCP1 NM_001008897 1 2.704 2.684 2.33 TUBA3E NM_207312 1 0.926 1.901 2.51 C1orf116 NM_023938 1 3.201 4.708 4.81 KLF6 NM_001008490 1 1.547 2.349 2.235 ABCD3 NM_002858 1 1.343 2.174 2.268 NT5DC3 NM_001031701 1 1.766 3.057 3.208 CENPN NM_018455 1 2.617 4.436 4.73 KIF20A NM_005733 1 1.729 2.162 2.208 TMPRSS2 NM_005656 1 5.732 11.34 10.4 GUCY1A3 NM_000856 1 2.274 2.331 2.347 MALT1 NM_006785 1 1.766 2.15 2.194 DHCR24 NM_014762 1 1.749 2.04 2.269 CLDN8 NM_199328 1 1.966 4.803 4.623 RAB4A NM_004578 1 1.473 2.291 2.278 SLC45A3 NM_033102 1 5.39 8.06 8.896 IGFBP3 NM_000598 1 2.388 1.762 1.802 DBI NM_020548 1 1.534 1.975 2.08 CSK NM_004383 1 1.527 2.452 2.671 RASD1 NM_016084 1 0.926 2.594 3.261 FAM107B NM_031453 1 1.786 2.586 2.48 C16orf44 NM_024731 1 0.979 2.101 2.298 CHKA NM_001277 1 1.34 2.041 1.845 MAGEA4 NM_001011548 1 2.248 2.083 1.898 NEDD4L NM_015277 1 1.945 2.435 2.333 NFKBIA NM_020529 1 1.46 1.959 2.175 TMEPAI NM_020182 1 2.142 2.548 2.698 KLK4 NM_004917 1 2.445 2.853 2.988 TBC1D8 NM_007063 1 1.038 2.207 2.095 GRHL2 NM_024915 1 1.928 3.509 3.319 ACSL3 NM_004457 1 1.607 2.399 2.196 FZD1 NM_003505 1 1.579 2.108 2.499 EHF NM_012153 1 2.997 3.024 2.866 ZNF350 NM_021632 1 1.504 2.214 2.045 MAP7D1 NM_018067 1 2.081 3.069 3.152 KRT73 NM_175068 1 0.926 3.054 3.179 TSC22D1 NM_006022 1 2.948 4.32 4.374 DNM1L NM_005690 1 1.368 1.918 2.182 UCK2 NM_012474 1 1.285 2.186 2.454 PAK1IP1 NM_017906 1 1.77 4.391 4.504 FAM13A1 NM_001015045 1 1.135 2.095 1.835 KRT8 NM_002273 1 0.951 2.299 2.549 SLC2A3 NM_006931 1 0.926 2.281 2.624 ERRFI1 NM_018948 1 3.055 8.661 8.572 MAFB NM_005461 1 1.991 2.622 2.873 CDC2 NM_001786 1 2.397 2.11 2.147 KCNN2 NM_021614 1 1.641 2.37 2.267 PPFIBP2 NM_003621 1 1.607 2.14 2.107 C19orf48 NM_199249 1 1.88 2.164 2.126 TIPARP NM_015508 1 2.184 13.53 14.39 SHROOM3 NM_020859 1 1.988 3.114 3.452 LAMA3 NM_000227 1 2.258 2.757 2.572 KIAA1754 NM_033397 1 0.957 1.822 2.172 FEN1 NM_004111 1 1.883 1.936 2.137 UGT2B11 NM_001073 1 2.613 1.958 1.931 ORM1 NM_000607 1 0.926 1.757 2.018 AMD1 NM_001033059 1 2 1.812 1.773 EAF2 NM_018456 1 1.166 2.92 3.285 LTB4DH NM_012212 1 2.159 1.76 1.873 RTN4 NM_007008 1 2.406 2.144 1.923 LYPLAL1 NM_138794 1 1.881 1.929 2.014 NKX3-1 NM_006167 1 5.869 8.877 9.301 ILF3 NM_004516 1 2.058 1.9 1.799 SRP9 NM_003133 1 2.051 1.824 1.761 ATAD4 NM_024320 1 2.276 2.08 1.957 TWIST1 NM_000474 1 1.483 1.988 2.117 MTMR9 NM_015458 1 1.592 2.633 2.657 RDH10 NM_172037 1 1.873 6.864 7.415 EPB41L4B NM_018424 1 1.895 2.339 2.136 MELK NM_014791 1 2.022 1.778 1.763 C6orf85 NM_021945 1 1.824 3.46 3.733 KIF22 NM_007317 1 1.479 2.056 2.272 FAM105A NM_019018 1 1.273 3.387 3.488 ZFP36 NM_003407 1 1.473 2.717 2.703 DUSP1 NM_004417 1 1.269 2.069 2.011 GADD45G NM_006705 1 1.301 3.141 3.508 MDC1 NM_014641 1 2.421 2.639 2.509 TNFRSF10B NM_003842 1 1.547 2.552 2.645 TACC2 NM_006997 1 1.658 2.383 2.438 LAMC1 NM_002293 1 1.648 2.082 2.13 STK39 NM_013233 1 1.843 2.252 2.271 ZBTB16 NM_001018011 1 6.314 10.41 10.13 TRIM36 NM_001017397 1 1.805 2.683 2.74 EDG7 NM_012152 1 1.412 1.838 2.038 TUBA1A NM_006009 1 1.991 1.835 2.095 CROT NM_021151 1 1.906 2.595 2.677 CDC7 NM_003503 1 1.402 1.976 2.001 CDC2 NM_001786 1 2.248 2.178 2.149 DNASE2B NM_021233 1 1.585 1.985 2.022 FAT NM_005245 1 1.762 2.076 2.284 LRRFIP2 NM_006309 1 1.624 2.548 2.521 TMEPAI NM_020182 1 4.69 6.857 6.814 MSX1 NM_002448 1 1.241 1.689 2.013 UHRF1 NM_013282 1 2.048 1.933 2.008 TYMS NM_001071 1 2.362 2.086 2.286 BBX NM_020235 1 1.018 1.979 2.041 PPAP2A NM_003711 1 2.445 3.111 2.802 PGM2 NM_018290 1 1.356 1.954 2.039 NCAPD3 NM_015261 1 1.703 5.299 5.528 ALDH1A3 NM_000693 1 2.334 4.126 3.95 BUB1B NM_001211 1 2.007 1.693 1.521 ERN1 NM_001433 1 1.625 3.226 3.651 SLC41A1 NM_173854 1 1.21 2.125 2.544 GHR NM_000163 1 2.347 2.55 2.677 TSC22D1 NM_006022 1 1.617 2.228 2.333 PACSIN1 NM_020804 1 1.074 1.818 2.06 TMEFF2 NM_016192 1 1.546 2.197 2.383 SGK NM_005627 1 7.005 17.66 16.81 PGC NM_002630 1 1.45 2.983 3.242 LONRF1 NM_152271 1 2.095 3.752 3.923 HMGCS2 NM_005518 1 3.799 3.092 3.452 SLC36A1 NM_078483 1 1.938 3.358 3.219 PXMP3 NM_000318 1 1.334 2.031 2.02 PRKCA NM_002737 1 1.43 2.747 2.574 ABCC4 NM_005845 1 1.344 2.132 1.858 PGAM1 NM_002629 1 2.328 2.105 2.076 IMPDH1 NM_000883 1 1.642 2.643 2.945 VCL NM_003373 1 1.09 2.181 2.345 CDC14B NM_003671 1 2.83 2.715 2.591 UGT2B10 NM_001075 1 2.049 1.683 1.562 ATP1A1 NM_000701 1 1.418 1.955 2.178 PPAP2A NM_003711 1 1.691 2.266 2.025 PPAP2A NM_003711 1 1.86 2.369 2.388 UGT2B15 NM_001076 1 2.611 2.071 1.959 IRS2 NM_003749 1 1.149 2.451 2.488 TNFAIP3 NM_006290 1 1.158 2.057 2.173 C1orf128 NM_020362 1 2.073 2.282 2.167 NSMAF NM_003580 1 1.289 2.159 2.234 ATAD2 NM_014109 1 2.485 3.57 3.775 LRIG1 NM_015541 1 2.277 5.072 5.082 EEF2K NM_013302 1 1.742 2.306 2.136 FBXO38 NM_030793 1 1.709 2.034 2.075 MARCH3 NM_178450 1 1.033 2.347 2.504 EDC3 NM_025083 1 1.73 2.359 2.345 ZNF189 NM_003452 1 1.127 2.482 2.4 CCT6A NM_001009186 1 2.045 1.782 1.813 RHOU NM_021205 1 6.075 20.37 19.97 DRB1 NM_152945 1 1.306 3.395 4.034 F2RL1 NM_005242 1 1.996 2.919 2.742 CLEC2D NM_001004419 1 2.444 3.397 3.265 DEGS1 NM_003676 1 1.992 2.229 2.344 APRIN 1 1.206 1.936 2.028 ZNF533 NM_152520 1 2.082 2.819 2.962 ATBF1 NM_006885 1 1.599 2.143 2.037 KCTD3 NM_016121 1 1.534 3.173 3.092 AADAT NM_016228 1 2.125 1.881 1.796 CACYBP NM_001007214 1 2.126 1.945 1.913 DBC1 NM_014618 1 2.689 1.776 1.987 SASH1 NM_015278 1 1.932 2.606 2.424 LRRC8A NM_019594 1 1.202 1.918 2.009 KLK3 NM_001030047 1 2.798 4.32 3.601

>2-fold downregulated at 4h TRPS1 NM_014112 1 0.562 0.487 0.426 C20orf46 NM_018354 1 0.413 0.36 0.327 SAMD11 NM_152486 1 0.71 0.493 0.549 TRIB1 NM_025195 1 0.64 0.556 0.476 LMO4 NM_006769 1 0.627 0.454 0.456 BAMBI NM_012342 1 0.651 0.452 0.399 MT1A NM_005946 1 0.496 0.48 0.419 REPS2 NM_004726 1 0.479 0.515 0.487 NRP1 NM_001024628 1 0.724 0.6 0.495 HOXB13 NM_006361 1 0.6 0.498 0.491 DUSP5 NM_004419 1 0.391 0.314 0.31 FLJ20054 NM_019049 1 0.469 0.37 0.3 SI NM_001041 1 0.429 0.422 0.436 NETO1 NM_138966 1 0.868 0.583 0.495 TULP4 NM_001007466 1 0.719 0.561 0.47 LPHN2 NM_012302 1 0.495 0.442 0.424 EPHA3 NM_005233 1 0.542 0.474 0.486 ATP2B1 NM_001001323 1 0.694 0.488 0.433 DDC NM_000790 1 0.573 0.507 0.458 GRB10 NM_001001549 1 0.625 0.461 0.424 ZBTB43 NM_014007 1 0.448 0.336 0.293 SLITRK3 NM_014926 1 0.229 0.145 0.115 TNFRSF19 NM_018647 1 0.433 0.655 0.59 OSR2 NM_053001 1 0.603 0.332 0.343 ENC1 NM_003633 1 0.636 0.509 0.463 BIRC3 NM_001165 1 0.567 0.31 0.304 NFKB1 NM_003998 1 0.782 0.5 0.549 MIPEP NM_005932 1 0.357 0.409 0.374 CAMK2N1 NM_018584 1 0.601 0.512 0.46 C6orf66 NM_014165 1 0.654 0.486 0.488 CXXC5 NM_016463 1 0.689 0.492 0.527 MYB NM_005375 1 0.519 0.391 0.404 RNF19 NM_015435 1 0.661 0.51 0.493 TDP1 NM_001008744 1 0.463 0.398 0.393 C4orf18 NM_001031700 1 0.534 0.404 0.341 C4orf18 NM_001031700 1 0.541 0.406 0.35 C12orf26 NM_032230 1 0.625 0.52 0.479 CCL2 NM_002982 1 0.483 0.376 0.388 MT1G NM_005950 1 0.384 0.334 0.309 EFNB2 NM_004093 1 0.526 0.412 0.371 PIK3R1 NM_181504 1 0.64 0.48 0.458 TLL1 NM_012464 1 0.451 0.33 0.266 SOX8 NM_014587 1 0.61 0.461 0.478 TSPAN1 NM_005727 1 0.412 0.46 0.442 C5orf30 NM_033211 1 0.484 0.328 0.277 CCDC59 NM_014167 1 0.714 0.473 0.438 SPRYD5 NM_032681 1 0.451 0.732 0.757 CCL20 NM_004591 1 0.576 0.339 0.344 TGM3 NM_003245 1 0.229 0.208 0.151 DAPK1 NM_004938 1 0.495 0.487 0.503 MBD3L2 NM_144614 1 0.279 0.327 0.332 MANEA NM_024641 1 0.596 0.464 0.394 SLC30A4 NM_013309 1 0.314 0.372 0.329 LRRN1 NM_020873 1 0.76 0.467 0.404 FBXO7 NM_001033024 1 0.526 0.496 0.493 SDCBP2 NM_015685 1 0.446 0.382 0.378 NRP1 NM_001024628 1 0.498 0.513 0.467 MT1X NM_005952 1 0.426 0.421 0.39 TFF3 NM_003226 1 0.26 0.304 0.255 PHF12 NM_001033561 1 0.762 0.555 0.497 ZNF294 NM_015565 1 0.931 0.539 0.469 OPRK1 NM_000912 1 0.406 0.22 0.167 DDEF2 NM_003887 1 0.541 0.431 0.384 PIGR NM_002644 1 0.442 0.513 0.475 CBLN2 NM_182511 1 0.529 0.369 0.313 ZIC2 NM_007129 1 0.683 0.368 0.302 COL5A2 NM_000393 1 0.433 0.403 0.415 ANKRD16 NM_001009941 1 0.665 0.477 0.47 EXPH5 NM_015065 1 0.84 0.488 0.468 BCHE NM_000055 1 0.362 0.314 0.29 PKIB NM_032471 1 0.6 0.497 0.403 C12orf60 NM_175874 1 0.629 0.442 0.422 SEMA6D NM_020858 1 0.493 0.478 0.411 KLHL1 NM_020866 1 0.722 0.505 0.445 ACPP NM_001099 1 0.558 0.482 0.418

>2-fold upregulated at 24h SORD NM_003104 1 1.336 1.639 3.538 CKS2 NM_001827 1 2.584 2.289 1.99 ASPH NM_004318 1 2.173 1.095 1.086 TSC2 NM_000548 1 1.291 1.922 2.159 KLK3 NM_001030047 1 3.002 5.616 7.554 PTTG1 NM_004219 1 2.938 2.311 2.106 MPP6 NM_016447 1 1.511 1.807 2.106 CDCA3 NM_031299 1 2.604 2.585 1.703 FKBP5 NM_004117 1 1.328 1.78 6.789 PSMC4 NM_006503 1 2.257 1.554 1.704 FLJ25416 NM_145018 1 1.626 2.624 3.324 ELOVL5 NM_021814 1 1.271 2.158 2.865 ELL2 NM_012081 1 1.565 2.15 5.83 PDIA6 NM_005742 1 1.47 1.789 2.022 SSR1 NM_003144 1 1.127 1.443 2.404 TROAP NM_005480 1 1.779 2.201 1.468 NDRG1 NM_006096 1 0.85 1.068 4.171 PEX10 NM_002617 1 1.269 1.693 3.159 CDK2 NM_001798 1 2.132 2.668 2.344 HSPC111 NM_016391 1 1.674 2.226 2.551 RAP1GAP NM_002885 1 0.858 1.16 2.049 OBFC2A 1 1.581 1.999 2.401 PPRC1 NM_015062 1 1.537 2.027 2.133 GSR NM_000637 1 1.535 1.557 2.184 CDC20 NM_001255 1 2.498 2.649 2.004 KCNN2 NM_021614 1 1.922 4.015 5.409 DNAJC9 NM_015190 1 1.683 2.118 1.937 CDCA5 NM_080668 1 3.358 4.64 3.588 C16orf61 NM_020188 1 1.304 1.87 2.444 MAD2L1 NM_002358 1 3.634 4.206 4.567 AURKB NM_004217 1 2.578 3.016 2.14 TCP1 NM_001008897 1 4.032 2.742 2.878 TUBA3E NM_207312 1 0.952 0.956 2.07 UGT2B28 NM_053039 1 1.409 3.055 8.812 C1orf116 NM_023938 1 1.122 1.342 2.059 PSMA6 NM_002791 1 1.67 1.726 2.149 DNM1L NM_005690 1 1.282 1.513 2.177 PUS1 NM_001002019 1 1.443 2.05 2.192 NT5DC3 NM_001031701 1 1.505 1.816 2.013 DC2 NM_021227 1 2.019 2.091 2.842 UCHL5 NM_015984 1 1.718 2.044 2.504 ZNF239 NM_005674 1 1.633 1.914 2.103 FANCI NM_018193 1 2.282 2.784 1.838 CENPN NM_018455 1 2.412 5.048 8.952 KIF20A NM_005733 1 2.653 2.825 2.158 CCNC NM_001013399 1 2.146 1.617 1.924 POLA2 NM_002689 1 1.631 2.134 1.886 IFI44 NM_006417 1 2.043 1.749 1.621 TMPRSS2 NM_005656 1 1.37 4.287 11.06 PSMA1 NM_002786 1 2.086 1.613 1.945 MALT1 NM_006785 1 1.31 1.678 2.208 RPS7 NM_001011 1 2.121 2.249 2.835 SLC35F2 NM_017515 1 1.345 1.429 2.68 TUBG1 NM_001070 1 1.618 2.02 1.912 C9orf152 NM_001012993 1 1.022 1.219 2.314 DHCR24 NM_014762 1 1.298 2.551 5.238 CDC25A NM_001789 1 2.172 2.977 2.916 HERPUD1 NM_001010989 1 1.21 1.184 2.307 SLC45A3 NM_033102 1 1.454 3.395 7.848 PPAPDC1B NM_032483 1 1.556 1.961 2.328 IGFBP3 NM_000598 1 2.848 2.277 1.076 HMBS NM_000190 1 2.21 1.931 1.945 STIL NM_003035 1 2.188 2.534 2.26 PDSS1 NM_014317 1 1.685 1.896 2.172 HSP90B1 NM_003299 1 1.145 1.539 2.045 CCNA2 NM_001237 1 2.607 2.706 2.343 FIGNL1 NM_001042762 1 2.122 1.978 1.769 DBI NM_020548 1 1.703 2.721 4.111 PPAT NM_002703 1 1.418 1.806 2.136 FAM83D NM_030919 1 2.791 3.018 2.568 RPL6 NM_000970 1 2.415 2.233 2.207 AK3L2 NM_001002921 1 1.786 1.773 2.356 MPHOSPH9 NM_022782 1 1.363 1.608 2.278 SKP2 NM_005983 1 1.725 2.223 2.162 MCM2 NM_004526 1 2.29 3.458 2.706 DTYMK NM_012145 1 2.619 2.551 2.258 MCM4 NM_005914 1 3.13 4.484 4.189 UBIAD1 NM_013319 1 1.382 1.753 2.096 MAGEA4 NM_001011548 1 2.276 1.801 1.645 NDC80 NM_006101 1 1.964 2.284 1.848 UBE2T NM_014176 1 2.521 2.881 2.802 IQGAP3 NM_178229 1 2.352 2.741 2.112 PIGW NM_178517 1 1.783 2.405 2.426 HAT1 NM_001033085 1 1.336 1.557 2.052 TMEPAI NM_020182 1 0.952 1.539 2.245 IMPA2 NM_014214 1 1.622 1.962 2.009 KLK4 NM_004917 1 1.506 2.125 2.699 BCAP29 NM_001008405 1 1.668 1.384 2.086 ACSL3 NM_004457 1 1.32 1.442 4.407 EHF NM_012153 1 2.442 2.197 2.726 TMED7 NM_181836 1 1.198 1.151 2.004 PPID NM_005038 1 1.608 1.704 2.14 BRP44 NM_015415 1 1.189 1.569 2.004 POLE3 NM_017443 1 1.749 2.201 2.041 DNASE2B NM_021233 1 1.357 1.49 2.123 TSC22D1 NM_006022 1 2.102 1.692 2.928 UCK2 NM_012474 1 1.679 2.021 2.011 TMSL8 NM_021992 1 2.207 2.123 1.917 PAK1IP1 NM_017906 1 1.983 2.43 4.022 HMG1L1 NM_001008735 1 2.094 1.953 1.687 GTF3A 1 1.719 2.028 2.306 TUBB NM_178014 1 2.529 2.435 2.154 KRT8 NM_002273 1 0.78 1.313 2.754 SLC33A1 NM_004733 1 1.403 1.381 2.105 SLC39A6 NM_012319 1 1.937 1.774 2.086 GFM1 NM_024996 1 1.094 1.457 2.479 DTL NM_016448 1 2.35 3.826 3.682 ZWINT NM_001005413 1 2.692 3.23 2.467 GLRX2 NM_016066 1 1.431 2.028 2.802 ERRFI1 NM_018948 1 0.971 1.052 3.207 CHEK1 NM_001274 1 1.82 2.123 2.058 HMMR NM_012484 1 3.027 3.038 2.414 TAF9 NM_001015891 1 1.964 1.722 2.014 TFB2M NM_022366 1 1.626 1.91 2.497 MPZL1 NM_003953 1 1.385 1.339 2.11 CDC2 NM_001786 1 4.44 3.806 3.407 ASF1B NM_018154 1 2.045 2.598 1.788 RAD51C NM_002876 1 1.988 2.093 2.021 STMN1 NM_005563 1 2.105 1.875 1.607 PPFIBP2 NM_003621 1 1.066 1.585 2.448 C19orf48 NM_199249 1 1.834 2.769 2.987 TOP2A NM_001067 1 2.773 2.896 2.328 CADPS2 NM_001009571 1 1.01 1.575 2.553 LDLR NM_000527 1 1.305 1.67 3.109 DDX39 NM_005804 1 1.823 2.043 2.178 PMM2 NM_000303 1 1.504 1.851 2.344 RRS1 NM_015169 1 1.535 1.941 2.16 FANCD2 NM_001018115 1 1.624 2.006 1.642 SEC24D NM_014822 1 1.262 1.445 2.735 RANBP1 NM_002882 1 2.418 2.295 2.307 TSPAN13 NM_014399 1 1.71 1.779 2.724 KDELR2 NM_006854 1 1.582 1.815 2.746 UAP1 NM_003115 1 1.083 1.609 2.873 CENPM NM_001002876 1 2.633 3.237 2.636 FEN1 NM_004111 1 2.431 2.876 2.387 UGT2B11 NM_001073 1 2.271 1.739 3.767 PDIA5 NM_006810 1 1.727 2.661 3.302 PTRH2 NM_001015509 1 1.425 1.81 2.347 ERGIC2 NM_016570 1 1.149 1.362 2.068 SLC25A19 NM_021734 1 1.835 2.4 2.259 AMD1 NM_001033059 1 2.421 1.742 1.962 RPS15 NM_001018 1 2.066 2.278 2.651 TRIP13 NM_004237 1 2.491 3.139 2.967 S100A11 NM_005620 1 1.378 1.718 2.124 PRC1 NM_003981 1 2.326 2.41 1.93 MLPH NM_001042467 1 1.266 1.413 2.089 RTN4 NM_007008 1 2.254 1.377 1.043 LYPLAL1 NM_138794 1 1.861 1.819 2.573 TACC3 NM_006342 1 1.986 2.455 1.805 POLQ NM_199420 1 1.875 2.655 1.934 MCM5 NM_006739 1 2.005 2.903 2.345 NKX3-1 NM_006167 1 1.321 2.648 3.877 FAM64A NM_019013 1 2.148 2.487 1.947 BCCIP NM_016567 1 1.669 1.553 2.068 BCCIP NM_016567 1 1.624 1.741 2.169 TMED2 NM_006815 1 1.289 1.556 2.145 SRP9 NM_003133 1 2.168 1.497 1.954 CHORDC1 NM_012124 1 1.949 1.948 2.056 FKBP11 NM_016594 1 1.804 2.548 3.259 HIST1H4C NM_003542 1 2.611 2.646 2.042 CDCA7 NM_031942 1 1.955 3.746 3.289 SMC4L1 NM_001002799 1 1.949 2.188 2.011 MCM10 NM_018518 1 1.663 2.485 2.337 KRT18 NM_000224 1 1.478 1.771 3.138 SERP1 NM_014445 1 1.248 1.43 2.338 MELK NM_014791 1 2.407 3.012 2.225 DPH2 NM_001039589 1 1.725 2.107 2.487 ACTG1 NM_001614 1 2.079 1.341 1.035 TIPIN NM_017858 1 1.77 2.028 1.87 ARL6IP6 NM_152522 1 2.394 2.085 1.893 BXDC2 NM_018321 1 1.889 2.154 2.23 UCHL3 NM_006002 1 1.58 1.94 2.282 IDH1 NM_005896 1 1.973 1.796 2.268 PPIL5 NM_152329 1 2.119 2.218 2.166 EVA1 NM_005797 1 1.11 1.674 2.03 SLC43A1 NM_003627 1 1.281 1.797 2.466 CENPM NM_001002876 1 2.326 2.596 1.939 CCNB2 NM_004701 1 2.646 2.974 2.208 FUSIP1 NM_006625 1 2.258 1.751 1.723 C1orf43 NM_015449 1 2.145 1.652 1.981 NOL5A NM_006392 1 1.794 2.232 2.139 RFC4 NM_002916 1 2.141 2.872 2.595 HNRPA3 NM_194247 1 2.028 1.512 1.178 NANS NM_018946 1 1.382 1.583 2.421 FADS1 NM_013402 1 1.471 1.822 2.847 ST6GALNAC1 NM_018414 1 0.964 2.636 5.219 RRP15 NM_016052 1 1.365 1.65 2.037 LAMC1 NM_002293 1 1.065 1.787 2.42 STK39 NM_013233 1 1.491 2.016 4.05 LOC400451 NM_207446 1 1.371 1.807 2.577 NOLC1 NM_004741 1 1.815 2.168 2.168 GLYATL2 NM_145016 1 1.391 1.63 2.199 MARS2 NM_138395 1 1.544 1.689 2.286 UGT2B7 NM_001074 1 1.879 1.83 3.516 GAL NM_015973 1 1.772 2.157 2.165 ZBTB16 NM_001018011 1 1.271 1.882 2.905 RNASE4 NM_002937 1 1.284 1.472 2.389 MCM7 NM_005916 1 2.292 2.822 2.456 EDG7 NM_012152 1 1.322 1.469 2.412 SMS NM_004595 1 1.31 2.032 3.939 TUBA1A NM_006009 1 3.225 2.953 2.659 RNASEH2A NM_006397 1 2.353 2.953 2.372 DDX21 NM_004728 1 1.798 1.926 2.459 AGR2 NM_006408 1 1.091 1.902 4.775 HM13 NM_030789 1 1.738 1.877 2.156 SDF2L1 NM_022044 1 1.084 1.398 2.033 GLRX2 NM_016066 1 1.473 1.728 2.321 SLBP NM_006527 1 1.872 2.118 2.197 CDC7 NM_003503 1 1.787 2.305 2.21 CDC2 NM_001786 1 4.105 3.434 3.285 B2M NM_004048 1 1.286 1.408 2.148 C7orf24 NM_024051 1 2.405 2.417 2.322 NOL1 NM_001033714 1 1.499 1.879 2.19 DNMT1 NM_001379 1 1.846 2.258 1.877 TPM3 NM_001043351 1 2.091 1.44 1.241 BOP1 NM_015201 1 1.421 1.922 2.096 EEF1B2 NM_001037663 1 2.223 2.026 2.343 PBK NM_018492 1 1.893 2.004 1.677 HK2 NM_000189 1 1.268 1.664 2.317 EEF1B2 NM_001037663 1 1.79 1.814 2.326 CDCA4 NM_017955 1 1.947 2.517 2.114 TMEPAI NM_020182 1 1.642 3.893 6.998 UHRF1 NM_013282 1 3.019 4.114 4.069 DNAJB9 NM_012328 1 0.98 1.133 2.154 AURKA NM_003600 1 2.155 2.278 1.845 LYAR NM_017816 1 2.064 2.699 2.823 TYMS NM_001071 1 4.148 4.454 3.734 ARF4 NM_001660 1 1.884 1.861 2.607 LCP1 NM_002298 1 1.519 1.905 2.062 NUSAP1 NM_016359 1 2.952 3.274 2.697 MTP18 NM_001003704 1 1.666 2.347 2.848 MCM3 NM_002388 1 1.623 2.283 1.997 SLC25A15 NM_014252 1 1.512 2.009 1.991 PPAP2A NM_003711 1 2.406 2.586 4.807 KRT18 NM_000224 1 1.57 1.705 3.846 ABHD2 NM_007011 1 1.382 1.763 3.47 PECI NM_006117 1 1.216 1.409 2.154 PRDX4 NM_006406 1 1.764 2.301 2.932 ELOVL1 NM_022821 1 1.62 1.964 2.198 NCAPD3 NM_015261 1 1.408 1.666 3.693 ALDH1A3 NM_000693 1 1.384 2.137 4.801 TRA16 NM_176880 1 1.672 2.139 1.836 IL8 NM_000584 1 2.088 1.371 0.956 PASK NM_015148 1 1.953 2.719 3.382 BUB1B NM_001211 1 2.391 2.972 2.455 FASN NM_004104 1 1.491 1.879 3.251 SNX5 NM_014426 1 2.445 1.973 2.108 DSCR2 NM_003720 1 1.863 2.13 1.896 RAD54L NM_003579 1 1.979 2.886 2.376 ABCE1 NM_001040876 1 1.408 1.777 2.091 GHR NM_000163 1 2.286 2.287 1.707 GINS2 NM_016095 1 2.797 3.923 3.451 ODC1 NM_002539 1 1.218 1.883 4.792 C12orf48 NM_017915 1 2.027 2.612 2.559 TSC22D1 NM_006022 1 1.07 1.26 2.13 TMEFF2 NM_016192 1 1.366 2.132 2.751 NCAPG NM_022346 1 2.632 2.952 2.53 PI4K2B NM_018323 1 1.569 1.943 2.076 H2AFX NM_002105 1 1.878 2.251 1.856 DLG7 NM_014750 1 2.6 2.755 2.339 NCAPD2 NM_014865 1 1.758 2.056 1.677 PGC NM_002630 1 1.323 3.17 5.282 TUBA4A NM_006000 1 1.563 2.09 2.093 HMGCS2 NM_005518 1 4.123 2.107 1.644 MSTO1 NM_018116 1 1.456 1.889 2.31 C16orf75 NM_152308 1 1.9 2.472 2.364 THOC4 NM_005782 1 2.176 1.986 1.725 HOMER2 NM_004839 1 1.307 2.648 5.181 ABCC4 NM_005845 1 1.076 1.47 2.36 PGAM1 NM_002629 1 2.655 2.187 2.253 CMTM7 NM_138410 1 1.727 2.191 2.238 FGFR3 NM_000142 1 1.957 2.108 1.886 IMPDH1 NM_000883 1 1.431 1.715 2.156 PCNA NM_002592 1 2.741 3.222 3.087 SEPP1 NM_005410 1 1.396 1.249 2.194 CCNE2 NM_057735 1 2.084 2.46 2.364 RPL29 NM_000992 1 2.43 2.523 3.174 UGT2B10 NM_001075 1 1.673 1.566 3.876 PDIA4 NM_004911 1 1.2 1.597 2.218 PPAP2A NM_003711 1 1.53 1.638 2.437 PPAP2A NM_003711 1 1.855 2.347 4.313 DCK NM_000788 1 2 2.168 2.034 E2F2 NM_004091 1 1.797 3.042 2.478 RFC5 NM_007370 1 2.296 2.963 2.806 ARMET NM_006010 1 1.329 1.796 2.449 HLA-DMA NM_006120 1 1.019 1.455 2.159 IFIT2 NM_001547 1 2.017 2.025 2.25 NMU NM_006681 1 2.165 1.94 1.845 STT3B NM_178862 1 1.621 1.574 2.253 ATAD2 NM_014109 1 3.106 4.188 4.692 HEATR2 NM_017802 1 1.39 1.681 2.054 HNRPAB NM_004499 1 1.852 2.225 2.208 THOC3 NM_032361 1 2.318 1.845 1.509 LRP8 NM_001018054 1 1.426 1.85 2.016 SCARB1 NM_005505 1 1.471 1.772 2.179 EXOSC3 NM_001002269 1 1.713 1.798 2.021 FBXO38 NM_030793 1 1.357 1.463 2.101 RAD51AP1 NM_006479 1 1.997 2.143 2.045 ANG NM_001145 1 1.131 1.469 2.429 UGDH NM_003359 1 1.201 1.42 2.822 SCD NM_005063 1 1.344 1.702 2.75 SAT1 NM_002970 1 0.926 1.278 2.35 TMEM79 NM_032323 1 1.099 1.631 2.576 EEF1E1 NM_004280 1 1.583 1.711 2.09 MYC NM_002467 1 2.126 2.308 2.953 CCT6A NM_001009186 1 3.152 2.331 2.715 DHRS3 NM_004753 1 0.921 1.193 2.521 EBNA1BP2 NM_006824 1 1.531 1.813 2.052 CNIH NM_001009551 1 2.058 1.572 1.628 KNTC1 NM_014708 1 2.271 3.016 2.426 RHOU NM_021205 1 1.544 1.93 3.884 PGM3 NM_015599 1 1.329 1.446 2.25 CDKN3 NM_005192 1 2.404 2.314 2.23 METTL1 NM_005371 1 1.513 1.91 2.011 C1orf80 NM_022831 1 2.105 1.369 1.099 STT3A NM_152713 1 1.236 1.435 2.1 ACTR3 NM_005721 1 1.273 1.614 2.123 CLEC2D NM_001004419 1 2.098 1.907 4.244 TK1 NM_003258 1 2.426 2.925 2.297 RRM1 NM_001033 1 1.855 2.048 1.843 SEC11C NM_033280 1 1.709 2.323 2.897 PRIM1 NM_000946 1 2.016 2.191 2.191 DEGS1 NM_003676 1 1.107 1.471 2.016 DEGS1 NM_003676 1 1.518 2.047 2.724 UBE2C NM_007019 1 3.384 3.506 2.606 PSD3 NM_015310 1 1.784 1.698 2.064 DUSP4 NM_001394 1 1.06 1.463 2.402 CCT7 NM_001009570 1 2.311 1.801 1.725 DSN1 NM_024918 1 1.576 2.172 1.635 DKFZp762E1312 NM_018410 1 1.734 2.028 1.571 GINS3 NM_022770 1 1.552 2.001 1.952 AADAT NM_016228 1 2.472 3.88 4.142 SRPRB NM_021203 1 1.183 1.657 2.361 CHPT1 NM_020244 1 1.442 1.898 3.19 C13orf27 NM_138779 1 1.782 2.159 1.861 CACYBP NM_001007214 1 2.628 2.234 2.209 FBXO5 NM_012177 1 2.445 2.862 2.478 HMGB2 NM_002129 1 2.195 2.26 2.009 DBC1 NM_014618 1 2.363 1.854 0.964 SASH1 NM_015278 1 1.598 1.49 2.142 KLK3 NM_001030047 1 1.35 2.625 3.545 KIAA0101 NM_001029989 1 3.804 3.937 2.903 TIMELESS NM_003920 1 2.1 2.33 1.883 DKFZP686A01247 NM_014988 1 1.215 1.702 2.232 ANLN NM_018685 1 1.839 2.364 2.207 SPAG5 NM_006461 1 2.49 2.477 2.068 CKS1B NM_001826 1 2.563 2.977 2.785 HEATR1 NM_018072 1 1.403 1.719 2.037 SSR2 NM_003145 1 1.678 1.621 2.491

>2-fold downregulated at 24h VGLL4 NM_014667 1 0.786 0.547 0.496 RLN2 NM_005059 1 0.862 0.521 0.469 CXCR7 NM_001047841 1 0.691 0.404 0.255 TRPS1 NM_014112 1 0.631 0.398 0.339 ITM2C NM_001012514 1 0.583 0.562 0.479 LDOC1 NM_012317 1 0.526 0.383 0.303 C20orf46 NM_018354 1 0.612 0.421 0.38 NCAM2 NM_004540 1 0.747 0.513 0.455 GNG7 NM_052847 1 0.64 0.519 0.493 EFNB3 NM_001406 1 0.844 0.604 0.489 MMP7 NM_002423 1 1.068 0.403 0.314 IRX3 NM_024336 1 0.649 0.423 0.291 SLC44A1 NM_080546 1 0.806 0.522 0.414 UGT2B17 NM_001077 1 2.475 0.884 0.581 UGT2B17 NM_001077 1 1.548 0.694 0.392 FAM63A NM_001040217 1 0.493 0.553 0.542 CLYBL NM_138280 1 0.892 0.624 0.499 PRAC NM_032391 1 0.748 0.605 0.49 LUZP2 NM_001009909 1 0.822 0.603 0.477 LAMB2 NM_002292 1 0.521 0.516 0.478 SMA4 NM_021652 1 0.767 0.531 0.362 LCN2 NM_005564 1 0.645 0.401 0.379 DUSP5 NM_004419 1 0.518 0.448 0.404 AGA NM_000027 1 0.769 0.613 0.488 GATS NM_178831 1 1.05 0.682 0.469 FLJ20054 NM_019049 1 0.602 0.396 0.249 SEMA6A NM_020796 1 1.072 0.626 0.331 SI NM_001041 1 0.442 0.444 0.4 MYRIP NM_015460 1 0.74 0.55 0.487 TULP4 NM_001007466 1 0.634 0.463 0.329 MYT1 NM_004535 1 0.609 0.585 0.469 TNFRSF21 NM_014452 1 1.129 0.647 0.406 EPHA3 NM_005233 1 0.581 0.485 0.346 SYTL2 NM_032379 1 1.073 0.563 0.462 C1orf53 NM_001024594 1 0.874 0.535 0.444 FKBP1A NM_000801 1 0.637 0.502 0.494 GUSBL1 NM_001033523 1 0.843 0.534 0.436 SMARCD3 NM_001003801 1 0.674 0.483 0.402 ATP2B1 NM_001001323 1 0.822 0.549 0.449 DDC NM_000790 1 0.526 0.276 0.144 DHRS13 NM_144683 1 0.541 0.497 0.524 CBFA2T2 NM_001032999 1 0.762 0.565 0.5 C7orf41 NM_152793 1 0.691 0.543 0.471 GRB10 NM_001001549 1 0.563 0.335 0.174 ZBTB43 NM_014007 1 0.61 0.475 0.314 LRRFIP1 NM_004735 1 0.424 0.47 0.485 SLITRK3 NM_014926 1 0.318 0.217 0.139 TNFRSF19 NM_018647 1 0.424 0.39 0.613 OSR2 NM_053001 1 0.729 0.598 0.415 ENC1 NM_003633 1 0.891 0.575 0.475 PCGF2 NM_007144 1 0.664 0.513 0.486 CAMK2N1 NM_018584 1 0.439 0.268 0.151 SERPINI1 NM_005025 1 0.715 0.473 0.473 C10orf65 NM_138413 1 0.654 0.485 0.534 CYP2C9 NM_000771 1 0.735 0.675 0.478 CYBASC3 NM_153611 1 0.584 0.486 0.433 PINK1 NM_032409 1 0.586 0.388 0.361 FBXO2 NM_012168 1 0.621 0.491 0.369 TDP1 NM_001008744 1 0.5 0.539 0.415 PPFIA2 NM_003625 1 0.722 0.441 0.345 HOXC9 NM_006897 1 0.749 0.636 0.497 COBL NM_015198 1 0.55 0.392 0.421 USP11 NM_004651 1 0.647 0.54 0.433 PLA2G2A NM_000300 1 0.187 0.114 0.116 C4orf18 NM_001031700 1 0.405 0.365 0.268 C4orf18 NM_001031700 1 0.489 0.372 0.272 SELENBP1 NM_003944 1 0.773 0.468 0.298 ENO2 NM_001975 1 0.705 0.665 0.491 NIPSNAP3A NM_015469 1 0.991 0.484 0.372 ATP1B1 NM_001001787 1 0.602 0.403 0.427 SPTAN1 NM_003127 1 0.555 0.525 0.484 C7orf38 NM_145111 1 0.495 0.573 0.502 HOXC4 NM_014620 1 0.585 0.466 0.365 TLL1 NM_012464 1 0.418 0.255 0.159 ANTXR1 NM_018153 1 0.765 0.553 0.491 ST7 NM_018412 1 0.857 0.492 0.304 ST7 NM_018412 1 0.764 0.436 0.262 SOX8 NM_014587 1 0.751 0.539 0.427 C21orf81 NM_153750 1 0.531 0.397 0.269 LMOD3 NM_198271 1 0.433 0.502 0.452 TIMP2 NM_003255 1 0.633 0.488 0.441 SPRYD5 NM_032681 1 0.361 0.376 0.804 GGTLA4 NM_080920 1 0.625 0.598 0.479 HIBADH NM_152740 1 0.75 0.551 0.445 SESN1 NM_014454 1 0.798 0.617 0.478 ZNF14 NM_021030 1 0.457 0.568 0.508 C21orf34 NM_001005732 1 0.614 0.483 0.457 TGM3 NM_003245 1 0.295 0.177 0.128 MBD3L2 NM_144614 1 0.275 0.205 0.186 MANEA NM_024641 1 0.613 0.495 0.429 MANEA NM_024641 1 0.992 0.54 0.45 NFAT5 NM_006599 1 0.784 0.576 0.359 SLC30A4 NM_013309 1 0.184 0.188 0.199 LRRN1 NM_020873 1 0.666 0.454 0.29 BTD NM_000060 1 0.617 0.575 0.492 C10orf33 NM_032709 1 0.708 0.627 0.439 HOXC13 NM_017410 1 1.017 0.663 0.481 FBXO7 NM_001033024 1 0.6 0.483 0.458 SDCBP2 NM_015685 1 0.786 0.441 0.245 OAZ2 NM_002537 1 0.567 0.468 0.44 TFF3 NM_003226 1 0.296 0.214 0.268 MAP1B NM_005909 1 0.976 0.678 0.478 RALGPS1 NM_014636 1 0.727 0.556 0.5 PI3 NM_002638 1 0.896 0.462 0.32 ZNF294 NM_015565 1 1.447 0.817 0.477 OPRK1 NM_000912 1 0.491 0.258 0.184 FKBP1A NM_000801 1 0.751 0.493 0.43 ST7 NM_018412 1 0.742 0.388 0.214 DDEF2 NM_003887 1 0.726 0.544 0.495 COLQ NM_005677 1 0.516 0.419 0.378 CPLX1 NM_006651 1 0.51 0.378 0.332 PIGR NM_002644 1 0.486 0.488 0.441 PLEKHB1 NM_021200 1 0.489 0.475 0.4 ZNF467 NM_207336 1 0.575 0.459 0.451 CBLN2 NM_182511 1 0.728 0.495 0.255 ZIC2 NM_007129 1 0.893 0.538 0.34 KIAA0247 NM_014734 1 0.738 0.582 0.466 COL5A2 NM_000393 1 0.475 0.468 0.422 TMEM59L NM_012109 1 0.524 0.419 0.338 TDRD1 NM_198795 1 0.463 0.556 0.518 BCHE NM_000055 1 0.437 0.439 0.396 PKIB NM_032471 1 0.753 0.538 0.392 PIK3IP1 NM_052880 1 0.698 0.451 0.374 HSD17B7 NM_016371 1 0.605 0.601 0.498 STK3 NM_006281 1 0.684 0.574 0.455 ACPP NM_001099 1 0.507 0.297 0.234 Supplementary Table 5. Androgen responsive genes in VCaP according to microarray expression analysis Ratio values

Official symbol RefSeq Acc# 0nM DHT 1.0nM DHT 10nM DHT 100nM DHT 4h: > 2-fold up-regulated at 4 h VPS37B NM_024667 1 1.074 2.031 2.31 KCNG1 NM_002237 1 1.397 8.471 9.753 ORM2 NM_000608 1 2.132 4.1 3.553 SSX2IP NM_014021 1 0.959 2.05 1.792 ACAD8 NM_014384 1 1.893 2.032 2.128 CNN3 NM_001839 1 2.466 4.128 4.222 MICAL1 NM_022765 1 1.728 2.347 2.977 AKAP7 NM_004842 1 2.983 3.797 3.087 FKBP5 NM_004117 1 7.375 8.55 7.112 RHOB NM_004040 1 2.102 5.241 5.467 ZNF18 NM_144680 1 1.479 2.049 2.371 LRRFIP2 NM_006309 1 2.309 2.534 2.141 ZNF165 NM_003447 1 2.367 2.863 2.774 AKTIP NM_001012398 1 1.514 2.492 2.135 GDPD5 NM_030792 1 0.87 3.049 3.42 THYN1 NM_001037304 1 2.576 3.519 3.341 NDRG1 NM_006096 1 1.536 1.873 2.211 SOX9 NM_000346 1 5.444 7.17 7.317 TMCC3 NM_020698 1 3.574 6.039 6.422 LIFR NM_002310 1 2.609 2.752 2.082 FUT4 NM_002033 1 1.327 2.056 1.758 IRX3 NM_024336 1 1.961 2.508 2.694 CDK2AP2 NM_005851 1 1.831 1.872 2.508 GOLGA4 NM_002078 1 1.742 2.224 1.911 PDCD4 NM_014456 1 2 2.769 2.428 RAD54B NM_012415 1 2.814 3.02 2.342 RALBP1 NM_006788 1 1.588 3.103 2.791 EDEM1 NM_014674 1 2.738 3.937 3.724 CREB3L2 NM_194071 1 2.409 3.523 3.597 SPRED1 NM_152594 1 2.431 4.254 3.835 PLEKHC1 NM_006832 1 1.184 2.036 1.635 SMOC2 NM_022138 1 1.684 2.151 1.839 WIPI1 NM_017983 1 2.719 3.564 3.611 PIGA 1 1.471 2.064 1.786 KIF20A NM_005733 1 1.637 2.028 1.993 FZD8 NM_031866 1 2.712 3.049 2.668 TMPRSS2 NM_005656 1 4.368 3.324 3.215 PTPRA NM_002836 1 1.218 2.199 2.233 MME NM_000902 1 1.628 3.216 2.734 HIPK2 NM_022740 1 1.605 1.755 2.072 MALT1 NM_006785 1 2.045 1.848 1.569 DNAJA4 NM_018602 1 1.555 2.19 2.229 FJX1 NM_014344 1 0.664 2.079 1.837 CLDN8 NM_199328 1 6.048 7.433 6.723 RAB4A NM_004578 1 1.915 2.077 1.581 BAG3 NM_004281 1 1.657 2.129 1.968 HERPUD1 NM_001010989 1 2.373 2.708 2.521 AMACR NM_014324 1 2.478 2.166 1.652 PVRL3 NM_015480 1 2.543 3.989 3.963 TRAF3IP2 NM_147200 1 1.135 3.214 3.147 DBI NM_020548 1 2.266 2.409 2.386 DNAJB1 NM_006145 1 1.52 1.847 2.077 TSPYL2 NM_022117 1 1.455 2.303 2.727 CSK NM_004383 1 1.588 2.269 2.509 RASD1 NM_016084 1 1.808 9.937 8.957 FAM107B NM_031453 1 1.618 2.676 2.566 KRT72 NM_080747 1 0.87 1.598 2.01 BAZ1A NM_013448 1 1.602 2.068 1.584 FLJ20273 NM_019027 1 1.31 3.403 3.04 C16orf44 NM_024731 1 2.081 3.632 4.023 DERL2 NM_016041 1 1.862 2.172 2.089 CHKA NM_001277 1 1.86 2.344 2.357 DEDD2 NM_133328 1 1.833 1.938 2.152 GNAI1 NM_002069 1 1.804 2.021 1.61 CAMSAP1L1 NM_203459 1 1.496 2.161 1.941 NFKBIA NM_020529 1 1.884 2.399 2.305 PHACTR2 NM_014721 1 1.663 3.548 2.887 MGC42630 1 1.532 2.06 1.718 TBC1D8 NM_007063 1 1.738 3.563 3.304 PPP3CC NM_005605 1 1.344 2.022 1.818 GRHL2 NM_024915 1 3.19 4.302 3.679 NAGK NM_017567 1 2.59 5.256 4.968 ACSL3 NM_004457 1 3.166 3.343 2.52 FZD1 NM_003505 1 1.739 2.017 1.888 MCFD2 NM_139279 1 1.507 2.102 1.612 ZNF579 NM_152600 1 1.619 2.202 2.542 KRT73 NM_175068 1 1.402 8.585 8.95 PEA15 NM_003768 1 1.74 2.163 2.256 ADAMTS8 NM_007037 1 0.87 2.384 3.01 SC5DL NM_001024956 1 2.015 2.139 1.911 CRY2 NM_021117 1 1.631 2.024 2.294 DNASE2B NM_021233 1 1.867 2.34 2.396 DKFZp434K191 NM_001029950 1 2.22 2.768 3.367 TSC22D1 NM_006022 1 1.74 2.644 1.566 PDHA1 NM_000284 1 1.307 2.119 2.293 UCK2 NM_012474 1 1.324 2.034 1.952 SPHAR NM_006542 1 2.067 1.977 1.77 GPR64 NM_005756 1 1.374 5.546 4.965 EPN2 NM_014964 1 1.224 2.133 2.085 PPP1CB NM_002709 1 1.711 2.121 1.638 SETBP1 NM_015559 1 1.74 3.098 3.068 KRT8 NM_002273 1 1.726 3.187 3.788 GRB10 NM_001001549 1 1.243 3.571 3.173 PSMD8 NM_002812 1 1.663 2.063 2.12 ZNF217 NM_006526 1 2.121 1.56 1.075 PKP2 NM_001005242 1 1.464 3.077 2.417 HS3ST1 NM_005114 1 1.93 3.538 3.826 ZNF649 NM_023074 1 2.1 2.146 2.01 SLC2A3 NM_006931 1 2.413 2.017 2.143 PRKX NM_005044 1 1.812 2.245 2.204 ERRFI1 NM_018948 1 11.25 15.85 16.22 CEBPD NM_005195 1 2.001 2.922 3.102 MAFB NM_005461 1 1.231 2.067 2.009 BVES NM_007073 1 1.296 2.511 2.414 CDO1 NM_001801 1 2.168 2.566 1.841 LOC205251 1 1.253 2.249 2.501 KIAA1727 NM_033393 1 1.29 4.019 3.201 GOT1 NM_002079 1 1.408 2.097 2.135 MAP2K4 NM_003010 1 1.751 2.644 2.258 ZDHHC13 NM_001001483 1 1.567 2.312 1.919 ZFAND5 NM_006007 1 1.316 2.417 2.1 TIPARP NM_015508 1 8.607 21.22 21.83 FOXO3 NM_001455 1 1.679 2.206 2.319 IRX4 NM_016358 1 2.445 2.476 2.513 SEC24D NM_014822 1 1.563 2.2 1.925 SHROOM3 NM_020859 1 2.615 2.843 2.53 ZNF341 NM_032819 1 1.549 1.897 2.101 EFNA1 NM_004428 1 1.551 2.221 2.014 GREB1 NM_014668 1 1.755 3.051 2.649 JPH1 NM_020647 1 1.605 2.483 2.1 MIS12 NM_024039 1 1.826 2.12 1.987 KIAA1754 NM_033397 1 1.394 3.022 3.201 EAF2 NM_018456 1 5.025 8.391 6.36 FER1L3 NM_013451 1 0.98 2.943 2.953 ELOVL2 NM_017770 1 1.821 2.688 2.488 USP33 NM_015017 1 1.86 2.497 2.392 ZNF813 NM_001004301 1 2.487 2.399 2.232 C17orf79 NM_018405 1 1.724 2.68 2.389 ANXA2 NM_001002857 1 0.983 2.047 1.409 ROR1 NM_005012 1 1.872 2.406 2.683 CHORDC1 NM_012124 1 1.952 2.297 1.898 PDLIM1 NM_020992 1 1.23 2.622 2.544 NAG8 NM_014411 1 1.692 2.527 2.806 FNBP1L NM_001024948 1 1.618 2.087 1.767 ANKMY2 NM_020319 1 1.615 3.689 3.078 MTMR9 NM_015458 1 2.162 2.813 2.556 RDH10 NM_172037 1 13.84 28.02 23.94 SOCS2 NM_003877 1 2.126 2.005 1.754 SERTAD2 NM_014755 1 1.642 3.132 2.937 ELF2 NM_006874 1 1.831 2.908 2.898 SOX7 NM_031439 1 0.824 2.118 1.877 PHF19 NM_001009936 1 2.971 7.524 8.035 PLEKHF2 NM_024613 1 1.863 2.059 1.258 ZNF615 NM_198480 1 2.639 2.417 2.4 MGC14376 NM_001001870 1 1.188 2.02 1.762 KIF22 NM_007317 1 2.022 1.692 1.613 COBL NM_015198 1 1.351 2.134 1.909 FAM105A NM_019018 1 5.105 7.523 5.515 PHACTR3 NM_080672 1 0.87 3.866 3.709 RBM14 NM_006328 1 1.55 2.024 2.245 GADD45G NM_006705 1 6.232 9.623 9.63 LOC147804 NR_003148 1 2.761 3.434 3.245 ST6GALNAC1 NM_018414 1 1.099 5.026 4.069 TNFRSF10B NM_003842 1 2.776 3.912 3.497 TACC2 NM_006997 1 2.179 3.636 3.195 LAMC1 NM_002293 1 2.135 1.754 1.822 KIAA1434 NM_019593 1 1.465 2.042 1.887 SLC39A14 NM_015359 1 2.075 2.495 2.296 KCNK3 NM_002246 1 0.828 3.57 3.435 CCDC41 NM_001042399 1 1.697 2.41 1.919 ZCCHC6 NM_024617 1 2.075 1.953 1.794 SELS NM_018445 1 1.853 2.211 2.053 SERPINE2 NM_006216 1 2.622 7.964 8.304 KLF15 NM_014079 1 1.978 3.418 2.965 ZBTB16 NM_001018011 1 2.179 1.531 1.653 CITED2 NM_006079 1 3.721 6.301 5.956 CKB NM_001823 1 2.066 2.207 2.602 NEFH NM_021076 1 2.057 2.328 2.328 LARP2 NM_018078 1 1.857 2.556 2.519 ATP1B1 NM_001001787 1 2.052 3.423 2.55 AGR2 NM_006408 1 4.894 11.58 13.07 PPM1B NM_001033556 1 1.767 1.956 2.322 SDF2L1 NM_022044 1 2.546 2.813 3.22 NDFIP2 NM_019080 1 1.424 2.338 1.993 CDC7 NM_003503 1 1.575 2.074 1.895 SIPA1L2 NM_020808 1 1.435 1.994 2.088 MERTK NM_006343 1 1.779 2.911 2.606 AQP3 NM_004925 1 1.781 2.658 3.14 CST1 NM_001898 1 1.566 2.781 3.76 IL20RA NM_014432 1 4.494 8.638 7.668 TPD52 NM_001025252 1 1.759 2.111 1.789 EHD4 NM_139265 1 1.271 2.42 2.672 CDK5RAP2 NM_001011649 1 1.223 1.902 2.099 IQCB1 NM_001023570 1 1.939 2.69 2.379 PREP NM_002726 1 1.436 3.009 2.911 MTHFD1L NM_015440 1 2.403 2.167 2.104 EIF2AK3 NM_004836 1 1.735 2.055 1.968 LRRFIP2 NM_006309 1 1.861 2.106 1.931 SMOX 1 1.925 2.84 2.816 DNAJB9 NM_012328 1 4.7 6.313 6.605 PPP2CB NM_001009552 1 2.026 2.527 1.91 BBX NM_020235 1 1.968 3.25 3.075 MPHOSPH10 NM_005791 1 1.722 2.311 2.07 LOC152485 NM_178835 1 1.373 2.221 2.222 SYVN1 NM_032431 1 2.494 2.938 2.882 PRKAB2 NM_005399 1 1.384 2.016 1.848 RALY NM_007367 1 1.359 2.653 2.989 LYN NM_002350 1 1.739 2.666 2.384 PECI NM_006117 1 2.058 2.338 2.186 KIF21A NM_017641 1 1.798 2.135 1.899 AGR3 NM_176813 1 2.242 4.18 3.429 NCAPD3 NM_015261 1 9.392 12.25 12.93 ALDH1A3 NM_000693 1 1.789 2.287 1.582 KLHL2 NM_007246 1 1.474 3.138 2.406 SESN1 NM_014454 1 2.182 4.261 4.351 ERN1 NM_001433 1 2.815 5.735 5.472 SLC1A4 NM_003038 1 1.665 2.058 2.324 SLC22A5 NM_003060 1 1.981 2.482 2.601 ODC1 NM_002539 1 2.003 2.215 2.16 C12orf23 NM_152261 1 1.583 2.046 1.739 TSC22D1 NM_006022 1 1.978 2.525 2.391 PACSIN1 NM_020804 1 2.19 7.451 7.786 ITLN2 NM_080878 1 0.87 2.735 2.73 MBOAT2 NM_138799 1 2.035 2.147 1.857 INTS6 NM_001039937 1 1.604 2.046 1.885 SGK NM_005627 1 6.723 10.5 10.28 TRAF5 NM_001033910 1 1.082 3.27 3.19 AFF4 NM_014423 1 1.461 2.065 1.316 LONRF1 NM_152271 1 1.617 2.112 1.81 SLC36A1 NM_078483 1 4.131 3.324 3.213 ATP1B1 NM_001001787 1 2.964 5.151 4.815 PXMP3 NM_000318 1 2.178 3.654 3.115 WFS1 NM_006005 1 1.484 1.747 2.051 OSBPL6 NM_032523 1 0.87 2.387 2.187 HSPH1 NM_006644 1 2.074 2.367 2.365 CITED4 NM_133467 1 0.87 2.907 3.209 ZNF614 NM_025040 1 1.944 2.225 2.008 CRY1 NM_004075 1 1.886 2.197 1.727 USP54 NM_152586 1 1.075 2.184 1.901 PART1 1 2.394 2.051 1.845 C9orf80 NM_021218 1 1.507 2.232 1.815 GRHL1 NM_014552 1 1.126 3.128 2.731 SH3TC1 NM_018986 1 1.267 2.392 2.491 IMPDH1 NM_000883 1 1.325 2.379 2.664 MUM1L1 NM_152423 1 2.248 2.091 1.618 VCL NM_003373 1 1.694 4.51 4.162 MAP1B NM_005909 1 2.517 4.545 4.25 PIAS1 NM_016166 1 1.835 2.127 2.001 NUDT11 NM_018159 1 2.002 2.168 1.97 ATP1A1 NM_000701 1 1.511 2.22 1.845 ATP1A1 NM_000701 1 1.668 2.753 2.869 PDIA4 NM_004911 1 2.169 2.214 2.61 FZD5 NM_003468 1 2.606 2.484 2.804 SNAP91 NM_014841 1 2.119 3.146 2.998 ZNF761 NM_001008401 1 2.347 2.539 1.706 TRIB2 NM_021643 1 2.582 1.828 1.995 IRS2 NM_003749 1 1.644 3.231 2.579 TNFAIP3 NM_006290 1 2.259 3.544 2.63 RPS6KA2 NM_001006932 1 1.725 3.04 3.383 ARMET NM_006010 1 2.464 2.645 2.729 CYP1A1 NM_000499 1 3.231 3.76 3.382 NSDHL NM_015922 1 1.917 2.592 2.627 STAU2 NM_014393 1 1.493 2.351 2.109 NSMAF NM_003580 1 2.148 2.767 2.722 GALNT1 NM_020474 1 1.403 2.111 1.776 KHDRBS3 NM_006558 1 1.249 2.183 2.206 LRIG1 NM_015541 1 3.159 7.161 6.645 SIM2 NM_005069 1 2.328 2.777 2.871 EEF2K NM_013302 1 1.701 2.323 2.4 ATP7B NM_000053 1 1.55 3.357 3.574 PTPN9 NM_002833 1 1.579 2.652 2.75 PIK3AP1 NM_152309 1 1.269 2.31 2.28 DNAJB6 NM_005494 1 1.571 2.05 1.811 RIN2 NM_018993 1 1.397 3.542 3.228 EDC3 NM_025083 1 3.924 5.108 6.26 PHF19 NM_001009936 1 1.49 2.464 2.546 ZNF189 NM_003452 1 4.382 10.07 9.591 HSPA5 NM_005347 1 2.218 2.097 2.283 RHOU NM_021205 1 9.275 10.99 11.33 PGM3 NM_015599 1 1.728 2.495 1.993 NSMCE4A NM_017615 1 1.567 2.089 1.903 DRB1 NM_152945 1 2.28 8.884 8.052 OFD1 NM_003611 1 1.704 2.57 2.471 CKAP2 NM_018204 1 1.608 2.05 1.512 MTHFD2 NM_001040409 1 1.83 2.219 2.23 TRIM52 NM_032765 1 1.422 1.794 2.054 DNAJB11 NM_016306 1 2.226 2.757 2.943 AFTPH NM_001002243 1 1.737 2.917 2.453 AFTPH NM_001002243 1 1.787 2.656 2.348 SPATA5L1 NM_024063 1 1.833 2.791 2.535 NETO2 NM_018092 1 1.621 2.468 2.218 ATBF1 NM_006885 1 1.641 2.446 2.054 DDX24 NM_020414 1 1.162 2.155 2.167 KCTD3 NM_016121 1 2.704 3.726 2.695 SELS NM_018445 1 1.765 2.268 2.214 SLC7A5 NM_003486 1 1.354 1.858 2.016 GINS3 NM_022770 1 1.858 2.295 2.224 C11orf54 NM_014039 1 1.789 3.781 3.041 IDI1 NM_004508 1 1.64 2.312 1.952 WDFY3 NM_014991 1 1.537 2.254 2.036 HYPE NM_007076 1 3.29 3.951 4.739 CRELD2 NM_024324 1 2.607 3.005 3.931 SASH1 NM_015278 1 1.812 2.398 1.717 ACSL1 NM_001995 1 1.211 2.503 2.165 F3 NM_001993 1 6.497 14.48 13.67

>2-fold downregulated at 4h E2F5 NM_001951 1 0.865 0.673 0.471 KLK3 NM_001030047 1 0.636 0.42 0.336 VWA1 NM_022834 1 0.62 0.445 0.594 TLCD1 NM_138463 1 0.582 0.408 0.43 DUSP2 NM_004418 1 0.388 0.324 0.384 P2RY11 NM_002566 1 0.604 0.479 0.523 THBS1 NM_003246 1 0.321 0.498 0.338 TBX3 NM_005996 1 0.679 0.394 0.409 ACOX2 NM_003500 1 0.635 0.507 0.474 C14orf43 NM_001043318 1 0.647 0.471 0.456 ASZ1 NM_130768 1 0.418 0.337 0.351 RPS6KA4 NM_001006944 1 0.521 0.411 0.42 UGT2B17 NM_001077 1 0.598 0.482 0.412 TRIB1 NM_025195 1 0.0594 0.0377 0.042 HOXA10 NM_018951 1 0.685 0.458 0.401 BAMBI NM_012342 1 0.395 0.715 0.726 ESRRA NM_004451 1 0.586 0.479 0.431 ALDOA NM_000034 1 0.748 0.537 0.422 FAM134B NM_001034850 1 0.676 0.364 0.325 GDF15 NM_004864 1 0.233 0.226 0.25 GUCY1A3 NM_000856 1 0.44 0.342 0.286 HOXB13 NM_006361 1 0.462 0.379 0.355 SLC45A3 NM_033102 1 1.345 0.514 0.46 TMEM45B NM_138788 1 0.55 0.405 0.481 IGFBP3 NM_000598 1 0.215 0.178 0.177 SEMA6A NM_020796 1 0.638 0.443 0.466 TARP NM_001003799 1 0.743 0.546 0.386 MYT1 NM_004535 1 0.535 0.371 0.376 ID4 NM_001546 1 0.483 0.328 0.317 PEO1 NM_021830 1 0.59 0.499 0.516 TNFRSF21 NM_014452 1 0.471 0.32 0.349 MAP3K5 NM_005923 1 0.726 0.478 0.507 SYTL2 NM_032379 1 0.596 0.565 0.466 C20orf177 NM_022106 1 0.471 0.362 0.29 CBR4 NM_032783 1 0.505 0.371 0.331 AR NM_000044 1 0.433 0.3 0.343 AR NM_000044 1 0.363 0.287 0.334 TMEPAI NM_020182 1 0.743 0.291 0.263 INHBB NM_002193 1 0.321 0.32 0.381 KIAA1683 NM_025249 1 0.448 0.446 0.53 PRKCD NM_006254 1 0.677 0.418 0.415 C8orf55 NM_016647 1 0.735 0.493 0.483 FOXN2 NM_002158 1 0.6 0.481 0.434 TP53INP1 NM_033285 1 0.373 0.167 0.15 TSR1 NM_018128 1 0.635 0.522 0.447 SH3RF1 NM_020870 1 0.675 0.477 0.398 PKIA NM_006823 1 0.509 0.411 0.322 ENC1 NM_003633 1 0.476 0.733 0.765 LYSMD2 NM_153374 1 0.819 0.585 0.433 CAPRIN2 NM_001002259 1 0.631 0.45 0.49 PPFIBP2 NM_003621 1 0.387 0.199 0.211 MMP10 NM_002425 1 0.385 0.28 0.26 FLJ32252 NM_182510 1 0.34 0.249 0.268 RRS1 NM_015169 1 0.648 0.522 0.463 RCC1 NM_001269 1 0.61 0.583 0.495 RORC NM_001001523 1 0.482 0.469 0.557 CGNL1 NM_032866 1 0.653 0.503 0.456 MNT NM_020310 1 0.486 0.362 0.448 UGT2B11 NM_001073 1 0.894 0.56 0.461 TXNRD1 NM_003330 1 0.656 0.437 0.445 GPR116 NM_015234 1 0.557 0.456 0.441 PRUNE2 NM_138818 1 0.537 0.385 0.345 CNKSR3 NM_173515 1 0.327 0.718 0.609 CGN NM_020770 1 0.607 0.425 0.419 IRF2BP2 NM_182972 1 0.394 0.393 0.466 SNRPN NM_003097 1 0.904 0.812 0.491 CRTAP NM_006371 1 0.802 0.569 0.444 FOXA1 NM_004496 1 0.823 0.413 0.347 RNF19 NM_015435 1 0.45 0.444 0.42 TUFT1 NM_020127 1 0.501 0.493 0.524 ALS2CR13 NM_173511 1 0.707 0.443 0.414 ELF3 NM_004433 1 0.462 0.383 0.419 ACTG1 NM_001614 1 0.736 0.605 0.319 GATA2 NM_032638 1 0.286 0.61 0.588 WDR21A NM_015604 1 0.539 0.435 0.517 ID3 NM_002167 1 0.414 0.24 0.22 KCNS3 NM_002252 1 0.863 0.492 0.496 ARID5B NM_032199 1 1.031 0.535 0.484 C21orf33 NM_004649 1 0.728 0.554 0.438 C1orf43 NM_015449 1 0.874 0.699 0.479 LOC400451 NM_207446 1 0.636 0.295 0.313 IQSEC1 NM_014869 1 0.61 0.477 0.484 AGTR1 NM_000685 1 0.353 0.352 0.418 CABLES1 NM_138375 1 0.661 0.468 0.556 EFNB2 NM_004093 1 0.444 0.277 0.254 PWWP2 NM_138499 1 0.558 0.383 0.422 CAPN5 NM_004055 1 0.532 0.408 0.405 PIK3R1 NM_181504 1 0.593 0.348 0.395 HOXC4 NM_014620 1 0.567 0.407 0.436 TMEPAI NM_020182 1 0.999 0.494 0.489 TM4SF1 NM_014220 1 0.688 0.333 0.334 TESK2 NM_007170 1 0.485 0.375 0.423 C22orf29 NM_024627 1 0.65 0.523 0.489 ID1 NM_002165 1 0.33 0.243 0.273 C5orf30 NM_033211 1 0.664 0.506 0.495 CEBPA NM_004364 1 0.391 0.462 0.557 PPM2C NM_018444 1 0.747 0.529 0.41 NRXN2 NM_015080 1 0.535 0.424 0.538 SMAD6 NM_005585 1 0.61 0.437 0.519 DAPK1 NM_004938 1 0.573 0.486 0.577 DFFB NM_001004285 1 0.743 0.5 0.594 ZNF503 NM_032772 1 0.161 0.108 0.127 FAM43A NM_153690 1 0.395 0.328 0.343 RIPK2 NM_003821 1 0.59 0.551 0.427 MCCC2 NM_022132 1 0.946 0.72 0.466 GADD45A NM_001924 1 0.306 0.337 0.33 CD55 NM_000574 1 0.67 0.478 0.494 H2AFJ NM_018267 1 0.455 0.453 0.538 AP1S1 NM_001283 1 0.631 0.475 0.345 AP1S1 NM_001283 1 0.62 0.543 0.497 C1orf115 NM_024709 1 0.58 0.435 0.478 GCNT1 NM_001490 1 0.672 0.302 0.28 CSTF3 NM_001033505 1 0.61 0.413 0.391 CD97 NM_001025160 1 0.703 0.497 0.482 SDC4 NM_002999 1 0.505 0.451 0.487 FLJ43339 NM_207380 1 0.869 0.49 0.464 ZNF30 NM_194325 1 0.662 0.445 0.396 RAI2 NM_021785 1 0.571 0.499 0.507 TMC5 NM_024780 1 0.73 0.382 0.381 FLJ26850 NM_001001687 1 0.736 0.393 0.357 MTAP NM_002451 1 0.861 0.496 0.499 MYC NM_002467 1 0.477 0.228 0.211 C15orf39 NM_015492 1 0.454 0.438 0.476 CABC1 NM_020247 1 0.602 0.473 0.562 PRIM1 NM_000946 1 0.622 0.472 0.384 RPLP0 NM_001002 1 0.883 0.701 0.493 ZNF533 NM_152520 1 0.542 0.348 0.294 CCT7 NM_001009570 1 0.797 0.696 0.453 NEDD9 NM_006403 1 0.486 0.428 0.503 C12orf34 NM_032829 1 0.628 0.481 0.584 NKX2-2 NM_002509 1 0.562 0.444 0.444 MRPL45 NM_032351 1 0.829 0.713 0.474 KLHL21 NM_014851 1 0.478 0.341 0.404 NEDD9 NM_006403 1 0.476 0.384 0.456 KLK3 NM_001030047 1 0.634 0.415 0.435 >2-fold upregulated at 24h ORM2 NM_000608 1 2.335 3.6 NDRG4 NM_020465 1 1.4 2.283 LDLRAD3 NM_174902 1 1.993 2.107 VWA1 NM_022834 1 2.01 1.81 MICAL1 NM_022765 1 2.876 3.662 FKBP5 NM_004117 1 6.184 6.603 HIST1H2BD NM_021063 1 1.528 2.135 DKFZP564J0863 NM_015459 1 2.626 3.235 CST3 NM_000099 1 2.045 2.712 SHC1 NM_003029 1 2.213 2.136 CRYAB NM_001885 1 1.91 3.33 DPAGT1 NM_001382 1 1.844 2.014 ATP2B4 NM_001001396 1 2.217 2.093 RAP1GAP NM_002885 1 1.911 2.088 LIFR NM_002310 1 2.176 2.291 CDK2AP2 NM_005851 1 1.873 2.171 P4HB NM_000918 1 2.128 2.361 C17orf62 NM_001033046 1 2.309 2.309 INSIG1 NM_005542 1 2.135 2.373 TRPT1 NM_001033678 1 2.07 2.287 AKT1S1 NM_032375 1 1.731 2.061 CLDN7 NM_001307 1 1.864 2.068 SMOC2 NM_022138 1 2.11 1.907 GNE NM_005476 1 2.267 2.812 PSMD1 NM_002807 1 2.074 2.201 ALDOA NM_000034 1 2.18 2.413 CREB3L4 NM_130898 1 2.645 2.645 ATP6V0D1 NM_004691 1 1.851 2.305 NUCB2 NM_005013 1 3.23 3.771 TMPRSS2 NM_005656 1 3.927 4.498 MME NM_000902 1 1.907 2.814 HIPK2 NM_022740 1 2.157 2.564 TFPT NM_013342 1 2.057 2.084 BCL2L1 NM_001191 1 2.045 2.237 HOXB13 NM_006361 1 2.187 1.925 DHCR24 NM_014762 1 3.961 4.795 CLDN8 NM_199328 1 2.643 2.785 CORO1B NM_001018070 1 3.769 4.322 SLC45A3 NM_033102 1 2.814 2.723 AMACR NM_014324 1 2.124 2.547 FKBP2 NM_004470 1 1.946 2.127 PVRL2 NM_001042724 1 2.456 2.558 DCXR NM_016286 1 2.601 2.693 EMP1 NM_001423 1 1.642 2.013 DBI NM_020548 1 3.841 4.465 UNQ501 NM_198536 1 1.859 2.429 FADS3 NM_021727 1 1.862 2.506 SUOX NM_000456 1 1.933 2.06 MYBPC1 NM_002465 1 1.948 2.126 CLK3 NM_001292 1 2.36 2.116 TMED9 NM_017510 1 2.22 2.699 FLJ20366 NM_017786 1 2.102 2.038 WWC1 NM_015238 1 1.89 2.146 SEC61B NM_006808 1 2.083 2.4 ACSL3 NM_004457 1 2.385 2.362 ASRGL1 NM_025080 1 2.099 2.355 MAP7D1 NM_018067 1 2.697 3.016 DKFZp434K191 NM_001029950 1 1.771 2.155 KRT8 NM_002273 1 1.873 2.491 ZNF217 NM_006526 1 2.977 2.664 SLC2A3 NM_006931 1 2.318 2.838 GLRX2 NM_016066 1 1.984 2.249 ERRFI1 NM_018948 1 1.889 2.412 SLC26A2 NM_000112 1 2.902 2.793 BCAR3 NM_003567 1 1.95 2.132 THOC5 NM_001002877 1 1.889 2.077 CANT1 NM_138793 1 2.137 2.168 NEK6 NM_014397 1 1.95 2.076 MBOAT5 NM_005768 1 2.47 2.584 LDLR NM_000527 1 2.461 3.017 SLC17A5 NM_012434 1 2.288 2.73 UAP1 NM_003115 1 2.004 2.083 STRA13 NM_144998 1 2.14 2.074 FER1L3 NM_013451 1 1.596 2.271 RFPL2 NM_006605 1 2.217 2.188 ATP2A2 NM_001681 1 1.785 2.009 ATAD4 NM_024320 1 3.135 3.528 COPE NM_007263 1 2.309 2.566 FKBP11 NM_016594 1 2.327 2.435 MAN2B2 NM_015274 1 2.688 2.873 COX17 NM_005694 1 2.048 2.192 SOCS2 NM_003877 1 2.272 2.557 TPD52L1 NM_001003395 1 1.846 2.123 CIB1 NM_006384 1 1.986 2.094 ATF5 NM_012068 1 1.815 2.215 C6orf85 NM_021945 1 2.005 2.048 AP1B1 NM_001127 1 2.15 2.142 IDH1 NM_005896 1 2.46 2.159 GPX2 NM_002083 1 1.885 2.159 MGC14376 NM_001001870 1 1.747 2.22 ATP6V0B NM_001039457 1 2.008 2.482 KIF22 NM_007317 1 2.087 1.917 GADD45G NM_006705 1 2.361 3.445 SERPINA3 NM_001085 1 11.24 24.56 LOC147804 NR_003148 1 2.675 2.767 PPM1E NM_014906 1 2.153 2.194 FADS1 NM_013402 1 3.37 5.164 ST6GALNAC1 NM_018414 1 2.849 2.777 ZCCHC6 NM_024617 1 2.153 2.211 GJB1 NM_000166 1 2.273 2.592 SEC24C NM_004922 1 1.685 2.019 ZBTB16 NM_001018011 1 1.911 2.075 CETN2 NM_004344 1 1.94 2.209 CITED2 NM_006079 1 2.188 2.263 CKB NM_001823 1 2.958 3.5 NEFH NM_021076 1 2.045 2.444 RAPGEF3 NM_006105 1 2.027 2.235 SIDT2 1 1.905 2.151 CDC42EP4 NM_012121 1 2.348 2.435 HRASLS3 NM_007069 1 1.777 2.159 GMPPA NM_013335 1 2.063 2.499 AGR2 NM_006408 1 3.486 4.707 SDF2L1 NM_022044 1 2.056 2.457 CST1 NM_001898 1 5.436 10.3 C1orf24 1 1.671 2.098 VPS26B NM_052875 1 2.405 2.527 IL20RA NM_014432 1 2.194 2.681 TMEM125 NM_144626 1 2.058 2.453 TM4SF1 NM_014220 1 2.46 2.712 LOC152485 NM_178835 1 2.024 2.028 SYVN1 NM_032431 1 2.104 2.326 TESC NM_017899 1 3.799 3.924 AP2S1 NM_004069 1 2.122 1.917 ENG NM_000118 1 2.056 1.808 NUDT22 NM_032344 1 2.161 2.349 NCAPD3 NM_015261 1 11.53 15.04 ALDH1A3 NM_000693 1 2.269 2.28 FASN NM_004104 1 3.038 2.994 SLC22A5 NM_003060 1 1.659 2.009 ODC1 NM_002539 1 3.086 3.435 SURF4 NM_033161 1 1.999 2.015 MBOAT2 NM_138799 1 2.149 1.856 CYB561D2 NM_007022 1 1.805 2.2 PGC NM_002630 1 2.17 3.088 HMGCS2 NM_005518 1 2.057 1.93 SLC39A7 NM_006979 1 1.914 2.438 ZDHHC16 NM_032327 1 1.889 2.098 FAM100B NM_182565 1 2.045 2.104 TGM2 NM_004613 1 1.901 2.015 HOMER2 NM_004839 1 2.199 2.461 TMC6 NM_007267 1 1.943 2.144 CORO2A NM_003389 1 2.005 1.714 CECR6 NM_031890 1 2.301 2.409 ATP1A1 NM_000701 1 2.477 1.978 ATP1A1 NM_000701 1 3.058 3.673 PDIA4 NM_004911 1 1.931 2.516 TMEM141 NM_032928 1 2.118 2.164 FZD5 NM_003468 1 1.951 2.306 ZNF761 NM_001008401 1 3.027 2.242 HYOU1 NM_006389 1 2.249 1.905 CD63 NM_001040034 1 1.969 2.182 DDEFL1 NM_017707 1 1.601 2.015 FLJ43339 NM_207380 1 2.251 2.363 YIPF1 NM_018982 1 2.908 2.911 LRIG1 NM_015541 1 2.484 3.07 FLJ26850 NM_001001687 1 3.944 4.368 S100P NM_005980 1 2.23 3.231 DPP3 NM_005700 1 2.528 2.412 ARPC3 NM_005719 1 2.232 2.693 CFDP1 NM_006324 1 2.016 1.997 GCAT NM_014291 1 2.259 2.047 SCD NM_005063 1 2.448 2.585 SAT1 NM_002970 1 3.273 3.797 RNF145 NM_144726 1 2.202 2.406 TMEM79 NM_032323 1 2.69 3.075 SREBF1 NM_001005291 1 2.059 1.886 XAB2 NM_020196 1 1.967 2.312 RHOU NM_021205 1 2.133 2.558 SEC11C NM_033280 1 2.209 2.566 NQO1 NM_000903 1 1.95 2.319 CDC42EP2 NM_006779 1 2.115 1.771 COPG NM_016128 1 1.69 2.285 PFKFB2 NM_001018053 1 2.058 2.089 MAGEH1 NM_014061 1 1.609 2.15 SERINC2 NM_178865 1 1.965 2.153 GMDS NM_001500 1 2.076 2.64 SLC27A3 NM_024330 1 4.029 4.668 CRELD2 NM_024324 1 1.872 2.407 C19orf10 NM_019107 1 2.209 2.608 ISGF3G NM_006084 1 1.919 2.089 RARRES3 NM_004585 1 5.34 7.39

>2-fold downregulated at 24h RPL13A NM_012423 1 0.548 0.497 CEBPG NM_001806 1 0.453 0.5 ATF3 NM_001030287 1 0.191 0.255 MASK NM_001042452 1 0.602 0.494 DUSP2 NM_004418 1 0.385 0.375 ITM2C NM_001012514 1 0.503 0.441 SFRS3 NM_003017 1 0.567 0.449 C20orf46 NM_018354 1 0.402 0.364 FZD6 NM_003506 1 0.489 0.455 ASB9 NM_001031739 1 0.516 0.457 ACTA2 NM_001613 1 0.407 0.322 THBS1 NM_003246 1 0.135 0.13 REEP1 NM_022912 1 0.483 0.367 NPY NM_000905 1 0.284 0.286 FCGRT NM_004107 1 0.526 0.464 NASP NM_002482 1 0.545 0.437 SFRS7 NM_001031684 1 0.548 0.484 METTL7A NM_014033 1 0.696 0.497 WEE1 NM_003390 1 0.565 0.385 ASZ1 NM_130768 1 0.328 0.319 SLC44A1 NM_080546 1 0.583 0.48 UGT2B17 NM_001077 1 0.471 0.383 SLC29A1 NM_004955 1 0.633 0.417 TRIB1 NM_025195 1 0.193 0.155 DSCR6 NM_018962 1 0.505 0.454 CLYBL NM_138280 1 0.406 0.368 CLYBL NM_138280 1 0.448 0.437 MAD2L1 NM_002358 1 0.742 0.482 FXYD5 NM_014164 1 0.317 0.277 PRAC NM_032391 1 0.429 0.363 IVNS1ABP NM_006469 1 0.681 0.483 ZNF239 NM_005674 1 0.586 0.49 FAM134B NM_001034850 1 0.41 0.299 ITM2A NM_004867 1 0.21 0.19 GUCY1A3 NM_000856 1 0.506 0.481 SRI NM_003130 1 0.561 0.462 C10orf38 NM_001010924 1 0.384 0.321 LRP16 NM_014067 1 0.431 0.338 DCC1 NM_024094 1 0.594 0.4 ALDH5A1 NM_001080 1 0.577 0.477 TMEM45B NM_138788 1 0.499 0.53 FBLN2 NM_001004019 1 0.504 0.491 FGFRL1 NM_001004356 1 0.551 0.474 IGFBP3 NM_000598 1 0.0484 0.0614 CAMK2B NM_001220 1 0.488 0.344 NPEPL1 NM_024663 1 0.566 0.48 BTG3 NM_006806 1 0.541 0.437 KBTBD11 NM_014867 1 0.552 0.468 ID4 NM_001546 1 0.427 0.344 CORO1C NM_014325 1 0.575 0.488 TNFRSF21 NM_014452 1 0.488 0.43 ZNF84 NM_003428 1 0.544 0.488 MCM2 NM_004526 1 0.608 0.337 PFAAP5 NM_014887 1 0.578 0.464 MCM4 NM_005914 1 0.633 0.281 SYTL2 NM_032379 1 0.192 0.187 CBR4 NM_032783 1 0.479 0.38 CCDC74B NM_207310 1 0.568 0.455 AR NM_000044 1 0.511 0.498 AR NM_000044 1 0.293 0.193 PIGW NM_178517 1 0.623 0.48 NPTX2 NM_002523 1 0.473 0.446 DDC NM_000790 1 0.184 0.18 TTR NM_000371 1 0.377 0.367 PKD2 NM_000297 1 0.578 0.455 POLR1E NM_022490 1 0.514 0.445 EIF3S5 NM_003754 1 0.564 0.498 C5 NM_001735 1 0.494 0.505 C7orf41 NM_152793 1 0.432 0.431 CEBPB NM_005194 1 0.405 0.473 KLHL3 NM_017415 1 0.538 0.484 TIGA1 NM_053000 1 0.363 0.334 KIAA1199 NM_018689 1 0.469 0.457 HES6 NM_018645 1 0.438 0.347 ZAK NM_016653 1 0.635 0.432 TMSL8 NM_021992 1 0.79 0.472 DTL NM_016448 1 0.535 0.33 PRSS3 NM_002771 1 0.477 0.507 PKIA NM_006823 1 0.613 0.499 CHGA NM_001275 1 0.487 0.481 MAFB NM_005461 1 0.29 0.293 ADD3 NM_001121 1 0.361 0.318 ADD3 NM_001121 1 0.459 0.447 ASF1B NM_018154 1 0.76 0.486 CAPRIN2 NM_001002259 1 0.415 0.336 UNG NM_003362 1 0.531 0.382 HNRPH3 NM_012207 1 0.629 0.475 GMNN NM_015895 1 0.633 0.356 MMP10 NM_002425 1 0.266 0.209 CAMK2N1 NM_018584 1 0.457 0.498 FANCD2 NM_001018115 1 0.845 0.495 CFD NM_001928 1 0.516 0.428 LAMA3 NM_000227 1 0.515 0.391 LASP1 NM_006148 1 0.407 0.437 RBM4B NM_031492 1 0.489 0.461 CENPM NM_001002876 1 0.847 0.414 HSD17B6 NM_003725 1 0.137 0.113 UGT2B11 NM_001073 1 0.67 0.497 GPR116 NM_015234 1 0.346 0.338 PRUNE2 NM_138818 1 0.424 0.35 SEPT3 NM_019106 1 0.445 0.328 HBQ1 NM_005331 1 0.377 0.283 CNKSR3 NM_173515 1 0.483 0.433 NAP1L1 NM_004537 1 0.676 0.432 NAP1L1 NM_004537 1 0.646 0.409 CHAF1B NM_005441 1 0.698 0.436 LTA4H NM_000895 1 0.325 0.267 CRTAP NM_006371 1 0.669 0.437 CDCA7 NM_031942 1 0.498 0.254 MCM10 NM_018518 1 0.627 0.498 MYL5 NM_002477 1 0.409 0.388 FLJ45803 NM_207429 1 0.344 0.236 RPL17 NM_000985 1 0.587 0.476 ACTG1 NM_001614 1 0.582 0.222 TNNT1 NM_003283 1 0.308 0.265 ARL6IP6 NM_152522 1 0.577 0.406 ID3 NM_002167 1 0.397 0.481 SNX10 NM_013322 1 0.484 0.394 RKHD2 NM_016626 1 0.628 0.479 FAM84B NM_174911 1 0.519 0.466 C4orf18 NM_001031700 1 0.359 0.305 C4orf18 NM_001031700 1 0.336 0.3 RPL37 NM_000997 1 0.46 0.419 ADM NM_001124 1 0.333 0.376 GLTSCR2 NM_015710 1 0.54 0.458 DMN NM_015286 1 0.503 0.483 SLC16A10 NM_018593 1 0.369 0.304 CTSF NM_003793 1 0.386 0.34 MID1 NM_000381 1 0.286 0.267 KIAA0367 NM_015225 1 0.642 0.49 LAPTM4B NM_018407 1 0.409 0.398 MCM7 NM_005916 1 0.77 0.498 AGTR1 NM_000685 1 0.383 0.374 IGFBP2 NM_000597 1 0.365 0.25 EFNB2 NM_004093 1 0.295 0.252 CADPS NM_003716 1 0.565 0.448 NMNAT2 NM_015039 1 0.48 0.463 PNCK 1 0.532 0.399 CMBL NM_138809 1 0.28 0.242 RELN NM_005045 1 0.51 0.472 PALLD NM_016081 1 0.423 0.412 TARBP1 NM_005646 1 0.526 0.448 GNG4 NM_004485 1 0.567 0.409 MLF1IP NM_024629 1 0.623 0.412 PIK3R1 NM_181504 1 0.592 0.495 BASP1 NM_006317 1 0.264 0.257 ANTXR1 NM_018153 1 0.352 0.268 WTAP NM_004906 1 0.844 0.479 ZYX NM_001010972 1 0.351 0.344 RPL14 NM_001034996 1 0.481 0.338 ST7 NM_018412 1 0.162 0.142 ST7 NM_018412 1 0.173 0.162 C6orf48 1 0.434 0.353 CSRP2 NM_001321 1 0.462 0.451 ACTB NM_001101 1 0.678 0.363 RAC1 NM_006908 1 0.624 0.472 CDCA4 NM_017955 1 0.572 0.397 UHRF1 NM_013282 1 0.658 0.366 STC2 NM_003714 1 0.319 0.335 TYMS NM_001071 1 0.658 0.289 CCDC74A NM_138770 1 0.563 0.449 ID1 NM_002165 1 0.295 0.497 DDIT3 NM_004083 1 0.44 0.504 RPL9 NM_000661 1 0.592 0.49 VPS13A NM_001018037 1 0.644 0.443 CD302 NM_014880 1 0.513 0.399 NRXN2 NM_015080 1 0.331 0.322 SMAD6 NM_005585 1 0.454 0.501 COLEC12 NM_030781 1 0.391 0.313 ERP27 NM_152321 1 0.402 0.444 C21orf34 NM_001005732 1 0.293 0.307 RPS29 NM_001030001 1 0.423 0.434 FSCN1 NM_003088 1 0.478 0.466 SCML1 NM_001037535 1 0.706 0.498 TBPL1 NM_004865 1 0.409 0.422 FAM133B NM_001040057 1 0.665 0.5 ZNF503 NM_032772 1 0.254 0.188 TMEM158 NM_015444 1 0.241 0.223 MGP NM_000900 1 0.248 0.224 MFGE8 NM_005928 1 0.423 0.413 PSIP1 NM_021144 1 0.609 0.367 CRMP1 NM_001014809 1 0.547 0.381 TMEM145 NM_173633 1 0.572 0.428 NUP54 NM_017426 1 0.722 0.492 PLCXD1 NM_018390 1 0.662 0.494 MCCC2 NM_022132 1 0.752 0.377 GADD45A NM_001924 1 0.378 0.325 C16orf75 NM_152308 1 0.633 0.367 CD55 NM_000574 1 0.464 0.459 UBE2L6 NM_004223 1 0.477 0.514 CMTM7 NM_138410 1 0.539 0.437 MT1X NM_005952 1 0.474 0.473 NMT2 NM_004808 1 0.451 0.44 RBM12 NM_006047 1 0.53 0.422 TFF3 NM_003226 1 0.444 0.381 EFHD2 NM_024329 1 0.461 0.409 PCNA NM_002592 1 0.697 0.405 GCNT1 NM_001490 1 0.62 0.496 CCNE2 NM_057735 1 0.535 0.335 PTGER4 NM_000958 1 0.406 0.396 FOS NM_005252 1 0.445 0.454 E2F2 NM_004091 1 0.607 0.317 DPYSL2 NM_001386 1 0.539 0.374 OGDHL NM_018245 1 0.552 0.431 TUB NM_003320 1 0.688 0.486 PCGF6 NM_001011663 1 0.602 0.492 ST7 NM_018412 1 0.372 0.363 PLA1A NM_015900 1 0.568 0.442 RPL27A NM_000990 1 0.763 0.469 KHDRBS3 NM_006558 1 0.227 0.218 CDK5R1 NM_003885 1 0.524 0.451 KCNQ2 NM_004518 1 0.507 0.364 NGFRAP1 NM_014380 1 0.476 0.36 BTF3 NM_001037637 1 0.551 0.453 DECR1 NM_001359 1 0.444 0.393 LEPREL2 NM_014262 1 0.385 0.334 RAD51AP1 NM_006479 1 0.626 0.369 C20orf27 1 0.48 0.388 FKSG30 NM_001017421 1 0.755 0.411 JUND NM_005354 1 0.46 0.478 DPYSL4 NM_006426 1 0.586 0.454 HOXA13 NM_000522 1 0.545 0.459 KNTC1 NM_014708 1 0.719 0.459 PLA2G7 NM_005084 1 0.325 0.288 CLEC2D NM_001004419 1 0.601 0.427 TK1 NM_003258 1 0.83 0.438 PRIM1 NM_000946 1 0.428 0.258 NUDT8 NM_181843 1 0.57 0.409 OSBPL8 NM_001003712 1 0.439 0.4 PPIA NM_021130 1 0.707 0.418 RPLP0 NM_001002 1 0.665 0.372 ZNF533 NM_152520 1 0.41 0.344 HNRPDL NM_005463 1 0.596 0.478 SFRP1 NM_003012 1 0.609 0.483 PSAT1 NM_021154 1 0.446 0.49 PRKD1 NM_002742 1 0.593 0.469 PELI2 NM_021255 1 0.37 0.335 RNASET2 NM_003730 1 0.376 0.328 KIAA0286 NM_015257 1 0.671 0.463 CYP4F8 NM_007253 1 0.496 0.505 NEDD9 NM_006403 1 0.5 0.471 RAB31 NM_006868 1 0.512 0.484 RCL1 NM_005772 1 0.548 0.425 BTG1 NM_001731 1 0.471 0.476 KIAA0101 NM_001029989 1 0.567 0.303 RGS11 NM_003834 1 0.525 0.382 PCP4 NM_006198 1 0.461 0.45 Supplementary Table 6. The number of genes in upregulated biological process categories in LNCaP-AR

ARmo-vs - ARhi-vs- LNCaP-pcDNA3.1 LNCaP-ARmo LNCaP-ARhi pcDNA3.1 pcDNA3.1 Number of Number of genes in the Number of Number of upregulated GO: Biological process - categories: category upregulated genes upregulated genes genes p-value: p-value:

GO:9987: cellular process 14421 80 193 276 < 0.0001 < 0.0001 GO:7154:cell communication: 4937 15 53 53 < 0.0001 < 0.0001 GO:7165:signal transduction: 3945 15 44 45 0.0003 0.0002 GO:7242: intracellular signaling cascade 1560 10 25 31 0.0173 0.0017 GO:19932: second-messanger-mediated signaling 289 6 5 19 0.7608 0.0141 GO:48015: phosphoinositide-mediated signaling 123 5 4 17 0.7342 0.014 GO:7582: physiological process 13611 79 190 272 < 0.0001 < 0.0001 GO:50875: cellular physiological process 12138 74 175 261 < 0.0001 < 0.0001 GO:7049: cell cycle 1030 19 17 68 0.7367 < 0.0001 GO:74: regulation of progression through cell cycle 666 13 12 43 0.84 < 0.0001 GO:278: mitotic cell cycle 316 11 8 37 0.4847 < 0.0001 GO:87: M phase of mitotic cell cycle 230 11 7 33 0.3361 0.0005 GO:7067: mitosis 228 11 6 32 0.2165 0.0008 GO:7059: chromosome segregation 66 3 2 12 0.6484 0.0136 GO:51301: cell division 249 11 6 32 0.2172 0.0008 GO:8283: cell proliferation 840 7 14 28 0.1243 0.0003 GO:16043: cell organization and biogenesis 2148 15 34 68 0.0063 < 0.0001 GO:6996: organelle organization and biogenesis 1185 10 13 43 0.5296 < 0.0001 GO:7010: cytoskeleton organization and biogenesis 558 6 7 22 0.7803 0.0022 GO:7017: microtubule-based process 191 4 4 17 1 0.0035 GO:226: microtubule cytoskeleton organization and biogenesis 88 2 0 12 0.1549 0.0053 GO:7051: spindle organization and biogenesis 30 1 0 11 0.3132 0.0012 GO:6810: transport 3101 16 57 52 0.004 0.0002 GO:45045: secretory pathway 264 0 11 9 0.0008 0.0025 GO:8152: metabolism 9139 61 116 199 < 0.0001 < 0.0001 GO:44238: primary metabolism 8310 59 116 189 < 0.0001 < 0.0001 GO:19538: protein metabolism 3693 33 57 92 0.0109 < 0.0001 GO:44267: cellular protein metabolism 3660 32 56 92 0.0101 < 0.0001 GO:6457: protein folding 259 4 9 17 0.1602 0.0038 GO:6139: nucleobase. nucleoside. nucleotide and nucleic acid metabolism 4087 24 36 88 0.12 < 0.0001 GO:6259: DNA metabolism 839 14 10 45 0.4108 < 0.0001 GO:6260: DNA replication 212 11 2 26 0.0112 0.0098 GO:6261: DNA-dependent DNA replication 108 6 0 13 0.013 0.0927 GO:6629: lipid metabolism 790 7 24 26 0.002 0.0008 GO:44255: cellular lipid metabolism 634 7 22 22 0.0048 0.0048 GO:8610: lipid biosynthesis 286 2 12 12 0.0068 0.0068 GO:6631: fatty acid metabolism 174 1 10 9 0.0058 0.0103 GO:9719: response to endogenous stimulus 412 6 7 21 0.7798 0.0062 GO:6974:response to DNA damage stimulus 364 6 5 21 0.7613 0.006 GO:6281: DNA repair 296 5 3 19 0.7219 0.0067

NOTE: The displayed categories were selected on bases that each category contained, at least, 10 upregulated genes, and GeneSpring GX v. 7.3.1 GO-browser indicated p<0.001. Supplementary Table 7. Up- and downregulated genes in LNCaP- ARhi compared to LNCaP-pcDNA3.1 in 1nM DHT at 24h Gene symbol RefSeq Acc# Ratio values >2-fold upregulated genes LPHN2 NM_012302.2 2.388 NDC80 NM_006101.1 2.063 ASPH NM_020164.2 2.284 DBC1 NM_014618.1 2.483 HNRPA1 NM_002136.1 2.006 MAGEA4 NM_001011550.1 3.734 ASF1B NM_018154.2 2.149 CMTM8 NM_178868.3 2.703 RAD54L NM_003579.2 2.08 SDCBP2 NM_080489.2 2.028 CDCA3 NM_031299.3 2.737 FANCI NM_018193.1 2.398 PRIM1 NM_000946.2 2.119 THOC3 NM_032361.1 2.593 C12orf48 NM_017915.1 2.203 SLC25A19 NM_021734.3 2.043 CENPM NM_024053.3 2.444 CAPZA1 NM_006135.1 2.332 FAM64A NM_019013.1 2.257 RAD51AP1 NM_006479.2 2.098 CDC2 NM_001786.2 4.819 CCDC34 NM_030771.1 2.345 NCAPG NM_022346.3 2.766 AURKB NM_004217.1 2.709 CCNA2 NM_001237.2 2.739 DLG7 NM_014750.3 2.732 CDC25A NM_001789.2 2.29 IQGAP3 NM_178229.3 2.585 MELK NM_014791.2 2.53 TMEM167 NM_174909.1 2.033 HNRPA3 NM_194247.1 2.128 CDCA7 NM_145810.1 2.054 NMU NM_006681.1 2.275 HMMR NM_012485.1 3.662 DTL NM_016448.1 2.47 ZWINT NM_032997.2 3.004 FEN1 NM_004111.4 2.555 MCM5 NM_006739.2 2.107 BUB1B NM_001211.4 2.512 CDKN3 NM_005192.2 2.822 FAM83D NM_030919.1 3.131 FBXO5 NM_012177.2 2.328 RNASEH2B NM_024570.1 2.099 STIL NM_003035.1 2.729 TUBA4A NM_006000.1 2.531 TACC3 NM_006342.1 2.972 KIF20A NM_005733.1 3.415 KNTC1 NM_014708.3 2.168 TNNT1 NM_003283.3 2.149 HMGB2 NM_002129.2 2.648 KPNA2 NM_002266.2 2.999 CDCA4 NM_017955.2 2.219 STMN1 NM_005563.3 2.041 SPAG5 NM_006461.3 3.215 CDC7 NM_003503.2 2.183 MAD2L1 NM_002358.2 3.748 GINS2 NM_016095.1 3.34 TRIP13 NM_004237.2 2.766 MCM2 NM_004526.2 2.997 TOP2A NM_001067.2 3.993 ALDH3A2 NM_001031806.1 2.274 PAGE1 NM_003785.3 2.908 FGFR3 NM_000142.2 2.244 RNASEH2A NM_006397.2 3.056 ATAD2 NM_014109.2 2.756 AURKA NM_198434.1 3.377 CDCA5 NM_080668.2 5.099 HSPA4 NM_002154.3 3.23 UGT2B28 NM_053039.1 3.619 MAGEA1 NM_004988.3 2.065 TCP1 NM_030752.2 2.517 CCNB2 NM_004701.2 4.841 UHRF1 NM_013282.2 3.478 CDC25B NM_212530.1 2.097 PPIL5 NM_152329.3 2.64 GAL NM_015973.2 2.266 CENPM NM_001002876.1 3.938 TMSL8 NM_021992.2 2.312 PI3 NM_002638.2 3.145 HSPC111 NM_016391.2 2.072 TK1 NM_003258.1 3.654 PTTG1 NM_004219.2 5.407 BCL2L12 NM_052842.2 2.393 HMGCS2 NM_005518.2 2.752 SPRY1 NM_199327.1 2.901 RFC5 NM_007370.3 2.686 NUSAP1 NM_016359.2 5.112 NUDT1 NM_198954.1 2.277 GGH NM_003878.1 2.49 HMG1L1 NM_001008735.1 2.204 UBE2C NM_181803.1 6.753 RPL27A NM_000990.2 2.944 MTP18 NM_016498.3 2.183 PRC1 NM_003981.2 3.972 TYMS NM_001071.1 4.651 MCM4 NM_005914.2 3.613 CDK2 NM_001798.2 2.102 TIMELESS NM_003920.2 2.215 WDR54 NM_032118.2 2.376 UBE2T NM_014176.1 2.527 UNG NM_080911.1 2.004 KIAA0101 NM_014736.4 4.119 CDC2 NM_033379.2 5.59 CKS1B NM_001826.1 2.412 CDC20 NM_001255.1 6.186 RPS15 NM_001018.3 2.48 PPIAL4 NM_178230.1 2.001 GAGE7 NM_021123.1 2.269 CKS2 NM_001827.1 3.621 C3orf28 NM_014367.3 2.116 PCNA NM_182649.1 2.92 H2AFX NM_002105.2 2.488 PTMA NM_002823.2 2.314 RPL29 NM_000992.2 2.67 CCDC34 NM_030771.1 2.189 NP NM_000270.1 2.031 RPS7 NM_001011.3 2.383 UQCRH NM_006004.1 2.049 DDX39 NM_005804.2 2.195 RPLP1 NM_001003.2 2.213 LDHB NM_002300.3 2.813 XAGE1 NM_020411.1 4.244 MCM7 NM_182776.1 2.539 RPL14 NM_003973.2 2.159 RPS21 NM_001024.3 2.038 RPL10A NM_007104.4 2.168 HIST1H4C NM_003542.3 3.179

>2-fold downregulated genes AMACR NM_203382.1 0.469 FLJ40113 NM_198079.1 0.469 PELI2 NM_021255.1 0.482 KIAA1324 NM_020775.2 0.404 PBEF1 NM_182790.1 0.441 SHROOM2 NM_001649.2 0.493 CAPN5 NM_004055.3 0.449 IL1RN NM_173842.1 0.438 FLJ45337 NM_207465.1 0.355 RLN2 NM_005059.2 0.481 HOXC4 NM_153633.1 0.459 TMSL3 NM_183049.2 0.294 C4orf18 NM_016613.4 0.458 TDP1 NM_018319.3 0.327 ARSD NM_001669.2 0.437 TBL1X NM_005647.2 0.492 TULP4 NM_020245.3 0.491 SLC30A4 NM_013309.4 0.249 COLEC12 NM_130386.1 0.258 NRIP1 NM_003489.2 0.497 RLN1 NM_006911.2 0.397 LAMP2 NM_002294.1 0.419 SSFA2 NM_006751.3 0.481 C4orf18 NM_016613.4 0.417 GSTT1 NM_000853.1 0.468 OCRL NM_000276.3 0.379 CXX1 NM_003928.1 0.463 CYBASC3 NM_153611.3 0.493 TSC22D3 NM_004089.3 0.481 COL4A5 NM_033381.1 0.484 TFIP11 NM_001008697.1 0.257 DEGS1 NM_003676.2 0.497 ATG3 NM_022488.3 0.498 MANEA NM_024641.2 0.345 MANEA NM_024641.2 0.31 LAMP2 NM_013995.1 0.494 LAMP2 NM_002294.1 0.428 COBLL1 NM_014900.3 0.443 TNFRSF19 NM_148957.2 0.426 HIST1H2BD NM_138720.1 0.498 COBL NM_015198.2 0.39 TMEFF2 NM_016192.2 0.313 PLEKHB1 NM_021200.1 0.471 DEGS1 NM_003676.2 0.489 GRB10 NM_005311.3 0.389 DDC NM_000790.2 0.385 Supplementary Table 8. Upregulated genes in LNCaP-ARhi compared to LNCaP-pcDNA3.1 in 1nM DHT at 24h, and expressed in similar or hirger levels in VCaP. Gene symbol Ref.Seq Acc. # Description SLC25A19 NM_021734.3 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 ASF1B NM_018154.2 ASF1 anti-silencing function 1 homolog B (S. cerevisiae) PRIM1 NM_000946.2 primase, polypeptide 1, 49kDa RAD54L NM_003579.2 RAD54-like (S. cerevisiae) RAD51AP1 NM_006479.2 RAD51 associated protein 1 NDC80 NM_006101.1 NDC80 homolog, kinetochore complex component (S. cerevisiae) FEN1 NM_004111.4 flap structure-specific endonuclease 1 CENPM NM_024053.3 centromere protein M THOC3 NM_032361.1 THO complex 3 FANCI NM_018193.1 Fanconi anemia, complementation group I FBXO5 NM_012177.2 F-box protein 5 KNTC1 NM_014708.3 kinetochore associated 1 MCM5 NM_006739.2 minichromosome maintenance complex component 5 FAM64A NM_019013.1 family with sequence similarity 64, member A CDC25A NM_001789.2 cell division cycle 25 homolog A (S. pombe) CDCA3 NM_031299.3 cell division cycle associated 3 MELK NM_014791.2 maternal embryonic leucine zipper kinase CDCA7 NM_145810.1 cell division cycle associated 7 C12orf48 NM_017915.1 chromosome 12 open reading frame 48 CCDC34 NM_030771.1 coiled-coil domain containing 34 AURKB NM_004217.1 aurora kinase B STMN1 NM_005563.3 stathmin 1/oncoprotein 18 BUB1B NM_001211.4 BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) IQGAP3 NM_178229.3 IQ motif containing GTPase activating protein 3 CCNA2 NM_001237.2 cyclin A2 ZWINT NM_032997.2 ZW10 interactor FGFR3 NM_000142.2 fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) DTL NM_016448.1 denticleless homolog (Drosophila) NMU NM_006681.1 neuromedin U CMTM8 NM_178868.3 CKLF-like MARVEL transmembrane domain containing 8 NCAPG NM_022346.3 non-SMC condensin I complex, subunit G DLG7 NM_014750.3 discs, large homolog 7 (Drosophila) CDCA4 NM_017955.2 cell division cycle associated 4 HMGB2 NM_002129.2 high-mobility group box 2 STIL NM_003035.1 SCL/TAL1 interrupting locus CDKN3 NM_005192.2 cyclin-dependent kinase inhibitor 3 (CDK2-associated dual specificity phosphatase) TACC3 NM_006342.1 transforming, acidic coiled-coil containing protein 3 MCM2 NM_004526.2 minichromosome maintenance complex component 2 SPAG5 NM_006461.3 sperm associated antigen 5 TRIP13 NM_004237.2 thyroid hormone receptor interactor 13 CDC7 NM_003503.2 cell division cycle 7 homolog (S. cerevisiae) GINS2 NM_016095.1 GINS complex subunit 2 (Psf2 homolog) CDC2 NM_001786.2 cell division cycle 2, G1 to S and G2 to M CDC25B NM_212530.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 5, mRNA. FAM83D NM_030919.1 family with sequence similarity 83, member D RNASEH2A NM_006397.2 ribonuclease H2, subunit A TUBA4A NM_006000.1 tubulin, alpha 4a TNNT1 NM_003283.3 troponin T type 1 (skeletal, slow) MAD2L1 NM_002358.2 MAD2 mitotic arrest deficient-like 1 (yeast) TMSL8 NM_021992.2 thymosin-like 8 HMMR NM_012485.1 hyaluronan-mediated motility receptor (RHAMM) GAL NM_015973.2 galanin TCP1 NM_030752.2 t-complex 1 KPNA2 NM_002266.2 karyopherin alpha 2 (RAG cohort 1, importin alpha 1) PPIL5 NM_152329.3 peptidylprolyl isomerase (cyclophilin)-like 5 ATAD2 NM_014109.2 ATPase family, AAA domain containing 2 KIF20A NM_005733.1 kinesin family member 20A TOP2A NM_001067.2 topoisomerase (DNA) II alpha 170kDa AURKA NM_198434.1 aurora kinase A UHRF1 NM_013282.2 ubiquitin-like, containing PHD and RING finger domains, 1 NUDT1 NM_198954.1 nudix (nucleoside diphosphate linked moiety X)-type motif 1 CDCA5 NM_080668.2 cell division cycle associated 5 HSPC111 NM_016391.2 hypothetical protein HSPC111 CDK2 NM_001798.2 cyclin-dependent kinase 2 HMGCS2 NM_005518.2 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) RFC5 NM_007370.3 replication factor C (activator 1) 5, 36.5kDa CENPM NM_001002876.1 centromere protein M HMG1L1 NM_001008735.1 high-mobility group (nonhistone chromosomal) protein 1-like 1 TK1 NM_003258.1 thymidine kinase 1, soluble TIMELESS NM_003920.2 timeless homolog (Drosophila) CCNB2 NM_004701.2 cyclin B2 UNG NM_080911.1 uracil-DNA glycosylase WDR54 NM_032118.2 WD repeat domain 54 UBE2T NM_014176.1 ubiquitin-conjugating enzyme E2T (putative) TYMS NM_001071.1 thymidylate synthetase NUSAP1 NM_016359.2 nucleolar and spindle associated protein 1 PRC1 NM_003981.2 protein regulator of cytokinesis 1 MCM4 NM_005914.2 minichromosome maintenance complex component 4 PTTG1 NM_004219.2 pituitary tumor-transforming 1 CKS1B NM_001826.1 CDC28 protein kinase regulatory subunit 1B KIAA0101 NM_014736.4 KIAA0101 RPL27A NM_000990.2 ribosomal protein L27a PPIAL4 NM_178230.1 peptidylprolyl isomerase A (cyclophilin A)-like 4 H2AFX NM_002105.2 H2A histone family, member X CCDC34 NM_030771.1 coiled-coil domain containing 34 UBE2C NM_181803.1 ubiquitin-conjugating enzyme E2C PCNA NM_182649.1 proliferating cell nuclear antigen CDC2 NM_033379.2 cell division cycle 2, G1 to S and G2 to M RPS7 NM_001011.3 ribosomal protein S7 PTMA NM_002823.2 prothymosin, alpha (gene sequence 28) CKS2 NM_001827.1 CDC28 protein kinase regulatory subunit 2 CDC20 NM_001255.1 cell division cycle 20 homolog (S. cerevisiae) DDX39 NM_005804.2 DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 RPLP1 NM_001003.2 ribosomal protein, large, P1 LDHB NM_002300.3 lactate dehydrogenase B MCM7 NM_182776.1 minichromosome maintenance complex component 7 RPL14 NM_003973.2 ribosomal protein L14 RPL10A NM_007104.4 ribosomal protein L10a HIST1H4C NM_003542.3 histone cluster 1, H4c Supplementary Table 9. AR-binding sites and p-values of four independent ONCOMINE studies for 56 upregulated genes in LNCaP-hiAR in 1.0 nM DHT1

p-value3,4

Yu_J Clin Oncol 2004/07/15 Varambally_Cancer Cell 2005/11/15 Vanaja_Cancer Res 2003/07/15 Tomlins_Prostate_Nat Genet 2006/12/28

Norm(23)-PC(64)-MetPC(25)5,6 BPH(6)-PC(7)-CR-Met-PC(6) Norm(8)-PC(27)-MetPC(5) BPH(22)-PIN(13)-PC(30)-MetPC(19) Gene All Met - 7 symbol Description ARB All Groups: Norm-PC PC-MetPC All groups: BPH-PC PC-CRPCMet All groups: Norm-PC PC-Met Groups: BPH-PC PC-MetPC CRPCMet ASF1B ASF1 anti-silencing function 1 N/A2 N/A N/A 0.051 0.16 0.228 9.63E-04 0.256 0.123 0.04 0.482 0.197 0.296 homolog B (S. cerevisiae) ATAD2 ATPase family, AAA domain Enhancer -45 kb N/A N/A N/A 3.90E-04 0.03 1.40E-04 0.009 2.00E-04 0.118 0.065 0.593 0.008 0.359 containing 2 AURKA aurora kinase A 1.10E-15 1.60E-04 2.00E-11 5.10E-06 0.018 0.01 1.10E-04 0.002 0.039 5.40E-06 0.098 0.007 0.591

AURKB aurora kinase B 8.80E-10 1.10E-04 2.60E-04 0.002 0.469 0.003 0.055 0.476 0.21 3.20E-04 0.208 2.40E-05 0.242

BUB1B BUB1 budding uninhibited by 1.60E-08 2.10E-06 0.005 6.00E-06 0.018 0.003 0.011 0.005 0.151 0.094 0.109 0.073 N/A benzimidazoles 1 homolog beta (yeast) C12orf48 chromosome 12 open reading Enhancer -145; + N/A N/A N/A 0.011 0.062 0.011 0.004 0.009 0.14 N/A N/A N/A N/A frame 48 6.5 kb; +144kb C19orf48 chromosome 19 open reading N/A N/A N/A 0.111 2.80E-04 0.146 0.01 6.30E-05 0.497 0.57 0.73 0.8 0.002 frame 48 CCNA2 cyclin A2 Enhancer -56 kb; 8.70E-12 0.089 1.40E-14 5.50E-06 0.021 6.70E-04 0.009 0.222 0.013 6.40E-05 5.20E-04 0.012 +31 kb CCNB2 cyclin B2 Enhancer +65 kb 3.00E-09 0.02 3.30E-05 1.60E-06 0.009 0.003 0.004 0.011 0.071 N/A N/A N/A N/A

CDC2 cell division cycle 2, G1 to S and Enhancer -46.7 kb; 3.50E-10 0.006 3.40E-06 6.30E-06 0.043 8.50E-04 2.60E-04 4.10E-04 0.023 0.434 0.671 0.21 N/A G2 to M +47.6 kb; +67.3 kb; +73.9 kb; +94.4 kb CDC2 cell division cycle 2, G1 to S and Enhancer -46.7 kb; 3.50E-10 0.006 3.40E-06 6.30E-06 0.043 8.50E-04 2.60E-04 4.10E-04 0.023 0.434 0.671 0.21 N/A G2 to M +47.6 kb; +67.3 kb; +73.9 kb; +94.4 kb CDC20 cell division cycle 20 homolog (S. Enhancer + 12.2 kb 0.001 0.569 3.7E-4 1.40E-04 0.411 0.003 0.032 0.468 0.017 N/A N/A N/A N/A cerevisiae) CDC25A cell division cycle 25 homolog A 5.40E-04 0.418 0.004 0.004 0.578 0.013 0.526 0.621 0.343 1.10E-04 0.003 0.013 0.194 (S. pombe) CDCA3 cell division cycle associated 3 N/A N/A N/A 9.70E-07 9.50E-04 0.001 1.30E-04 0.004 0.065 N/A N/A N/A N/A

CDCA4 cell division cycle associated 4 N/A N/A N/A 0.001 0.247 0.026 2.00E-04 0.112 0.065 N/A N/A N/A N/A

CDCA5 cell division cycle associated 5 N/A N/A N/A 0.001 0.695 4.50E-04 0.012 0.022 0.18 6.90E-04 0.23 9.20E-07 0.672

CDCA7 cell division cycle associated 7 Enhancer +19.5 kb; N/A N/A N/A 0.062 0.268 0.046 0.253 0.205 0.269 0.73 0.167 0.081 0.553 +68 kb; +137 kb CDK2 cyclin-dependent kinase 2 Enhancer -57 kb 7.20E-05 0.12 4.60E-05 0.142 0.3 0.233 0.604 0.382 0.377 N/A N/A N/A N/A

CDKN3 cyclin-dependent kinase inhibitor Enhancer -40.5 kb; - 5.60E-08 0.63 6.60E-06 2.70E-07 0.01 0.002 0.009 0.047 0.063 0.338 0.417 N/A N/A 3 (CDK2-associated dual 64 kb; -191 kb; + specificity phosphatase) 68 kb CENPM centromere protein M N/A N/A N/A 1.10E-05 0.017 0.009 0.003 0.026 0.078 0.026 0.856 0.046 0.048

CKS1B CDC28 protein kinase regulatory 0.017 0.454 0.048 0.031 0.018 0.025 0.006 0.466 0.008 0.076 1.90E-04 0.029 0.39 subunit 1B DLG7 discs, large homolog 7 6.90E-05 0.044 0.002 7.00E-08 6.20E-04 0.003 0.002 0.005 0.081 9.30E-05 0.012 0.002 0.298 (Drosophila) DTL denticleless homolog N/A N/A N/A 3.80E-04 0.111 0.02 0.04 0.017 0.121 0.281 1.40E-04 8.50E-07 0.042 (Drosophila) FAM64A family with sequence similarity N/A N/A N/A 4.30E-05 0.283 6.90E-04 0.135 0.244 0.34 0.015 0.069 0.001 0.083 64, member A FAM83D family with sequence similarity N/A N/A N/A 0.095 0.185 0.023 0.218 0.513 0.553 0.001 0.005 0.274 0.752 83, member D FANCI Fanconi anemia, Enhancer -70 kb; - 0.029 0.937 0.049 4.00E-04 0.01 0.029 0.008 0.094 0.061 0.007 0.006 0.263 0.63 complementation group I 113 kb; Exon; +199 kb FEN1 flap structure-specific N/A N/A N/A 0.001 0.289 0.03 7.20E-04 0.004 0.112 0.426 N/A N/A N/A endonuclease 1 GINS2 GINS complex subunit 2 (Psf2 N/A N/A N/A 0.02 0.07 0.004 0.233 0.048 0.712 N/A N/A N/A N/A homolog) HMGB2 high-mobility group box 2 Enhancer +279 kb 1.90E-08 0.079 4.40E-05 0.004 0.196 0.02 0.036 0.077 0.187 N/A N/A N/A N/A

HMGCS2 3-hydroxy-3-methylglutaryl- Enhancer -81.6 kb 0.746 0.02 0.002 0.472 0.771 0.517 0.995 0.526 0.615 0.768 0.986 0.229 0.713 Coenzyme A synthase 2 (mitochondrial) HMMR hyaluronan-mediated motility Enhancer -26 kb 6.80E-10 0.057 3.20E-07 5.10E-07 0.004 0.002 0.001 6.20E-04 0.148 0.318 0.103 0.063 0.098 receptor (RHAMM) IQGAP3 IQ motif containing GTPase Enhancer -44 kb N/A N/A N/A 0.199 0.129 0.1 0.528 0.795 0.401 N/A N/A N/A N/A activating protein 3 KIF20A kinesin family member 20A Enhancer +89.6 kb N/A N/A N/A 1.30E-08 0.001 0.002 0.008 0.032 0.083 N/A N/A N/A N/A

MAD2L1 MAD2 mitotic arrest deficient-like Enhancer -254 kb 2.50E-04 0.009 4.30E-06 2.00E-05 0.011 0.039 0.008 0.005 0.19 0.464 0.753 0.445 0.276 1 (yeast) MCM4 minichromosome maintenance Enhancer -225 kb 0.371 0.015 0.006 0.001 0.543 0.003 0.054 0.018 0.128 1.40E-07 2.00E-07 0.004 0.029 complex component 4 MELK maternal embryonic leucine Enhancer -219; + 1.60E-05 0.083 0.001 1.10E-06 0.037 0.001 0.008 0.007 0.08 N/A N/A N/A N/A zipper kinase 87 kb; +246 kb NCAPG non-SMC condensin I complex, Enhancer +38 kb N/A N/A N/A 2.50E-05 0.003 0.003 0.011 0.021 0.118 N/A N/A N/A N/A subunit G NDC80 NDC80 homolog, kinetochore Enhancer -81 kb; - 0.943 0.803 0.828 0.621 0.319 0.778 0.797 0.755 0.851 0.431 0.289 0.194 0.001 complex component (S. 120 kb; +85 kb; cerevisiae) +196 kb NMU neuromedin U 0.009 0.241 0.022 0.009 0.471 0.002 0.165 0.055 0.644 N/A N/A N/A N/A

NUSAP1 nucleolar and spindle associated Enhancer -100 kb N/A N/A N/A 9.20E-08 1.60E-04 0.001 6.40E-05 0.001 0.025 3.90E-04 0.015 0.213 0.368 protein 1 PCNA proliferating cell nuclear antigen Enhancer +139 kb 0.051 0.659 0.011 0.01 0.655 0.061 0.002 0.005 0.097 0.023 0.349 0.15 0.802

PRC1 protein regulator of cytokinesis 1 Enhancer -12.5 N/A N/A N/A 1.60E-05 0.023 0.004 0.002 0.003 0.068 8.00E-06 0.179 1.00E-04 0.114

PRIM1 primase, polypeptide 1, 49kDa Enhancer -6.8 kb 0.001 0.069 0.016 0.003 0.857 0.013 0.007 2.70E-04 0.201 0.046 0.133 0.522 0.871

PTTG1 pituitary tumor-transforming 1 Enahncer -21 kb 8.30E-11 0.106 1.00E-06 2.70E-06 0.007 0.003 4.70E-04 0.033 8.50E-04 4.90E-06 0.225 1.00E-05 0.355

RAD51AP1 RAD51 associated protein 1 0.551 0.119 0.449 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A

RAD54L RAD54-like (S. cerevisiae) Enhancer -108 kb 9.20E-09 0.035 2.00E-10 8.60E-04 0.947 0.002 0.305 0.791 0.106 N/A N/A N/A N/A

RNASEH2A ribonuclease H2, subunit A Enhancer +17 kb 0.026 0.555 0.039 6.00E-05 0.062 0.007 0.017 0.113 0.168 N/A N/A N/A N/A

SPAG5 sperm associated antigen 5 Enhancer -7.2 kb 2.50E-06 1.20E-07 0.062 2.80E-05 0.245 0.001 0.368 0.713 0.335 N/A N/A N/A N/A

STIL SCL/TAL1 interrupting locus 1.40E-07 3.90E-06 0.037 3.00E-05 2.60E-06 0.047 0.004 4.70E-06 0.966 0.037 0.077 0.81 0.111 THOC3 THO complex 3 Enhancer -26 kb N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A

TK1 thymidine kinase 1, soluble Enhancer -197 kb 6.50E-09 0.003 3.70E-04 8.70E-07 0.008 5.40E-04 0.002 0.42 0.038 1.60E-05 0.032 3.60E-04 0.068

TOP2A topoisomerase (DNA) II alpha 1.40E-14 5.10E-06 1.30E-06 2.00E-07 0.011 5.50E-05 0.007 0.018 0.046 7.30E-05 0.014 0.001 0.134 170kDa TYMS thymidylate synthetase Enhancer -30 kb 0.109 0.285 0.419 0.003 0.223 0.027 0.003 0.148 0.014 0.313 0.068 0.292 0.42

UBE2C ubiquitin-conjugating enzyme Enhancer +41 kb 2.50E-10 8.00E-04 1.20E-07 4.50E-06 4.70E-04 0.004 0.028 0.279 0.115 0.693 0.18 0.344 N/A E2C UHRF1 ubiquitin-like, containing PHD N/A N/A N/A 1.20E-05 0.053 0.007 0.017 0.054 0.138 N/A N/A N/A N/A and RING finger domains, 1 ZWINT ZW10 interactor Enhancer -164 kb 7.00E-13 7.80E-09 5.00E-04 9.10E-06 0.003 0.012 6.80E-04 2.20E-04 0.095 0.002 0.06 0.413 0.803

1the criterias for selecting genes into the table: 1) genes were 2-fold upregulated in 1.0 nM DHT in LNCaP-ARhi compared to -pcDNA3.1, and 2) genes were 2-fold upregulated in LNCaP-ARhi 1nM compared to 0 DHT at 24h, adn 3) genes were 1.5- fold upregulated in LNCaP-ARhi at 4h by DHT, and 4) genes were expressed in equal level or more in VCaP compared to LNCaP-ARhi.

2N/A = data not available 3bold = gene significantly upregulated in the study group 4 italic = gene significantly downregulated in the study group 5Norm = Normal prostate; BPH = benign prostate hyperplasia; PIN = Prostatic Intraepithelial Neoplasia; PC = hormone-naive primary prostate cancer; Met = metastases, CRPCMet = metastatic castration prostate cancer sample; 6number of samples in the group is given in the bracket 7ARB = AR binding site according to ChIP on chip