JOURNAL OF BACTERIOLOGY

VOLUME 123 * NUMBER 2 * AUGUST 1975 EDITORIAL BOARD L. LEON CAMPBELL, Editor-in-Chief (1980) UniversitY of Delaware, Newark S. G. BRADLEY, Editor (1979) ALLEN T. PHILLIPS, Editor (1980) Virginia Commonwealth Universit,r Richmond Pennsylvania State University, University Park, Pa. ROY CUIRTISS Ill, Editor (1979) CARL SCHNAITMAN, Editor (1978) University of Alabama, Birmingham Universitr of Virginia, Charlottesville ELIZABETH McFALL, Editor (1980) H. E. UMBARGER, Editor (1976) New York University, New York, N. Y. Purdue UniversitY, Lafayette, Ind.

James N. Adams (1976) A. T. Ganesan (1975) M. L. Morse(1975) Arthur 1. Aronson (1976) Howard Goldfine (1975) Eugene W. Nester (1975) Gad Avigad (1977) Harry Gooder (1975) Donld P. Nierlich (1977) Stephen D. Barbour (1976) Joseph S. Gots (1975) Richard P. Novick (1976) W. Lane Barksdale (1975) Harlyn 0. Halvorson (1975) Michio Oishi (1977) M. E. Bayer (1975) George Hegeman (1977) L. Nicholas Ornston (1975) Blaine Beaman (1976) Donald R. Helinski (1975) Leo Parks (1976) Claire M. Berg (1976) Charles E. Hielmstetter (1975) Martin Pato (1975) Richard S. Berk (1977) James A. Hoch (1977) Paul V. Phibbs (1976) Judith S. Bond (1977) Joy Hochstadt (1977) Lewis 1. Pizer (1975) Winfried Boos (1976) Joseph T. Holden (1975) Charles C. Remsen III (1976) Herbert W. Boyer (1975) Stanley C. Holt (1977) William S. Reznikoff (1976) Jean Brenchley (1977) H. Ronald Kaback (1975) Antonio H. Romano (1976) George H. Brownell (1977) Sam Kaplan (1977) Robert Rownd (1977) Richard 0. Bern (1977) David E. Kennell (1977) Harold L. Sadoff (1977) Donald B. CIewell (1977) Bruce C. Kline(1977) M. R. J. Salton (1975) Stanley N. Cohen (1976) Robert L. Lester (1975) Irwin H. Segel (1976) Ronald S. Cole (1977) Hiliel S. Levinson (1975) Jane K. Sttlow (1975) S. F. Conti (1975) E. C. C. Lin (1975) Helen Jean Shadomy (1975) Nicholas R. Cozzarelli (1975) James S. Lovett (1977) Stanley K. Shapiro (1976) John E. Cronan, Jr. (1977) K. Brooks Low (1976) Simon Silver (1976) Walter J. Dobrogosz (1976) P. T. Magee (1977) W. R. Sistrom (1975) Patrick R. Dugan (1977) Michael H. Malamy (1975) Lucile Smith (1975) Alan D. Elbein (1976) Manley Mandel (1975) DonaldJ. Tipper (1975) Stanley Falkow (1975) Robert E. Marquis(1976) David White (1977) Gerald R. Fink (1975) Neil H. Mendelson (1975) Clifford A. Woolfolk (1976) W. R. Finnerty (1976) Gene E. Michaels (1977) Howard Zalkin (1976)

Robert A. Day, Managing Editor Gisdea Pollock, Director ofEditorial Services Linda Illig, Production Editor 1913 1St., N. W., Washington, D.C. 20006 EX OFFICIO

Helen R. Whiteley, President (1975-1976) Harlyn 0. Halvorson, Vice-President (1975-1976) J. Melisen Joseph, Secretary Brinton M. Miller, Treasurer

The Journal of Bacteriology, a publication of the American defective copies, availability of back issues, lost or late pro') Society for Microbiology, 1913 1 St., N.W., Washington, D.C. disposition of submitted manuscripts, an,d general editcraI 1913 20006, is devoted to the advancement and dissemination of fund- matters should be directed to the ASM Publications Office, amental knowledge concerning bacteria and other microorga- I St., N.W., Washington, D.C. 20006 (area 202 833-94! i} nisms. The Journal is published monthly, and the twelve numbers are divided into four volumes per year. The subscription price Second class postage paid at Washington. D.C. 2000b, anr. at is S85 (Postal Union Countries, S87: other foreign, S88) per year. additional mailing offices. Single copies are $8.00 (foreign, S8.25). Mcmbers of the Amer- Made in the United States of America. 0 ican Society for Microbiology may receive the Journal as part of Copyright 1975, American Society for Microbiology. their dues. Correspondence relating to subscriptions, reprints, All Rights Reserved. Author Index

Achtman, Mark, 505 Hanlon, Joan, 761 Patte, J. C., 391 Allen, Jane S., 693 Halvorson, Harlyn O., 497 Petrotta-Simpson, T. R., 516 Assimos, Katherine, 637 Hattman, Stanley, 768 Potvin, Barry W., 604 Hespell, Robert B., 481 Probst, Irmelin, 436 Bacha, Patricia, 546 Hirose, Akiko, 678 Ptashne, Kay, 687 Ballou, D. L., 616 Hirt, William E., 642 Benson, Spencer, 759 Hsieh, L. K., 463 Reiner, Albey M., 530 Blackwell, M. M., 743 Hu, Sylvia, 687 Reiners, John J., Jr., 620 Bonewald, Renate, 658 Hull, Richard A., 775 Rittenberg, Sydney C., 481 Borne, F., 391 Rivera-Ortiz, Jose M., 537 Boy, E., 391 Ingram L. O., 743 Rood, Julian I., 419 Bulla, Lee A., Jr., 598 Inselburg, J., 739 Rosen, Dalia, 765 Burris, R. H., 537 Kaplan, Nathan O., 704 Cassan, M., 391 Kelleher, Raymond J., Jr., 604 Schwesinger, M. D., 724 Cassio, D., 584, 589 Koshland, D. E., Jr., 557 Scocca, J. J., 666 Chang, H. C.-P., 755 Kovarik, Agnes, 693, 752 Sena, Elissa P., 497 Cherest, H., 428 Senior, Peter J., 407 Cohen, Stanley N., 687 Lai, C.-H., 747 Shapiro, James, 546, 759 Condon, Seamus, 570 Leahy, John, 546 Solioz, Marc, 651 Cronan, John E., Jr., 492 Sorger, G. J., 755 Li, Steven Shoei-Lung, 761 Spence, K. D., 516 Davidson, Norman, 687 Lin, Chi Chung, 637 Surdin-Kerjan, Y., 428 de Robichon-Szulmajster, H., Listgarten, M. A., 747 428 Macy, Joan, 436 Talmadge, J. E., 516 Drews, G., 449, 456 Mahler, Henry R., 637 Tanaka, Teruo, 771 Dyer, J. C., 755 Makula, R. A., 523 Taylor, Barry L., 557 Mandelbaum-Shavit, Frede- Toepfer, Melany, 546 Edelman, Marvin, 765 rika, 400 Markbreiter, Keith, 546 Vary, J. C., 463 Fan, David P., 717 Marrs, Barry, 651 Vestal, J. Robie, 642 Fennewald, Michael, 546 Mathien, Y., 580 Finn, Gerald J., 570 Matsuhashi, Michio, 678 Walker, James R., 693, 752 Finnerty, W. R., 523 May, Maureen S., 768 Waller, J. P., 580 Fogel, Seymour, 497 Mayer, H., 449, 456 Weckesser, J., 449, 456 Fromme, I., 449, 456 Miozzari, Guiseppe F., 481 Weisblum, Bernard, 771 Galun, Esra, 765 Morse, Stephen A., 631 Weisberg, Laurie J., 492 Gardner-Eckstrom, Heidi L., Mosteller, Raymond D., 750 Welch, Juliet W., 497 717 Mulkins, G. J., 755 Wilkinson, Russel G., 419 Gibson, Jane, 471 Winter, Daryl B., 631 Goebel, Werner, 658 Nickerson, Kenneth E., 598 Goldstein, Ruth V., 750 Nieder, Matthew, 546 Yamada, Masao, 678 Gooder, Harry, 604 Nordstrom, Kurt, 443 Yanofsky, Charles, 761 Gottschalk, Gerhard, 436 Novick, R. P., 724 Yen, Huei-Che, 651 Grossowicz, Nathan, 400 Nunn, William D., 492 You, Kwan-sa, 704 Grund, Alan, 546 Gustafson, Ralph A., 693 Oka, A., 739 Zalkin, Howard, 620 Gustafsson, Petter, 443 Olson, G. J., 743 Zoon, Kathryn C., 666 INSTRUCTIONS TO AUTHORS Submit manuscripts in duplicate (original and one Abstract. An Abstract appears at the beginning ot copy) to ASM Publications Office, 1913 I St., N.W., each paper in the Journal. The Abstract should not Washington, D.C. 20006. exceed 250 words. General policy. Any manuscript submitted must be Literature Cited. In the text, references are cited a report of unpublished original research, which is by number. The Literature Cited section should be not being considered for publication elsewhere. Each typed in alphabetical order, bv tirst author, and rnanuscript should present the results of an independ- numbered. Names ot journals are abbreviated ac- ent, cohesive study; "series" papers are discouraged. cording to Bibliographic Guide for Editors & Authors Papers that deal primarily with a method should not (American Chemical Society, 1974). Citations of ab- be submitted. stracts, theses, "unpublished data,.' personal com- The "editorial style" of the Journal essentially fol- munication," and "in press" will not be accepted in lows the CBE Style Manual (3rd ed., AIBS. 1972). the Literature Cited. For bacterial genetic systems, the recommendations Tables. Each table should be typed on a separate of Demerec et al. (Genetics 54:61, 1966) should be page. The data should be arranged so that columns ot followed, with the limited modifications of Taylor and like material read down, not across. Explanatory foot- Trotter, Sanderson, and Low (Bacteriol. Rev. 36:504- notes are permitted, but detailed descriptions of the 524; 558-586; and 587-607, 1972). For yeast, Chlamy- experiments are not. The materials and methods used domonas, and several fungal species, symbols such to gain the data should properly remain in the section as those given in Handbook of Microbiology (1974, of that name. A. I. Laskin and H. A. Lechevalier, eds., The Chemi- Figures. A complete set of figures. preferably cal Rubber Co., Cleveland, p. 665-711, Vol. IV) should glossy photographs, should accompany each of' the be employed. For genetic nomenclature, use italics two copies of the manuscript. Graphs (submit as photo- consistently for genotype symbols. Greater leniency graphs) should be finished drawings not needing can be allowed for phenotypic symbols. However, further artwork or type-setting. Absolutely no part of they should not be italicized. The preferred convention a graph should be typewritten (except the legend, is to use the same three-letter symbols in Roman type which should be typed on a separate page). Most with the first letter capitalized. graphs will be reduced to one-column width, and all Biochemical nomenclature, including abbrevia- elements in the drawing should be prepared to with- tions and symbols, should follow the recommenda- stand this reduction. The legend of the figure should tions of the IUPAC-IUB Commission on Biochemical provide enough informat ion so that the figure is Nomenclature (CBN) and the Instructions to Authors understandable without reterence to the text. Ex- of J. Biol. Chem. and Arch. Biochem. Biophys. (first perimental details from Materials and Methods issue of each year), which are based upon the CBN should not be repeated in figure legends. Figure ordi- Recommendations and contain a list thereof. Re- nate and abscissa scales, and table column headings, prints of these Recommendations and advice on bio- should avoid ambiguous use of numbers with expo- chemical and chemical nomenclature are available nents (see p. 140, CBE Style Manual). from the NRC Office of Biochemical Nomenclature Nomenclature of microorganisms. In general, the (W. E. Cohn, Director), Biology Division, Oak nomenclature presented in Bergey's Manual of Deter- Ridge Nat'l. Lab., Box Y, Oak Ridge, Tenn. 37830 minative Bacteriology (8th ed., 1974) is used. If an (phone: 615-483-8611, Ext. 3-7514). Normally, abbre- author challenges this nomenclature, his own judg- viations (except those of standard units of measure- ment will be followed, but the name in Bergey's Man- ment and symbols of the elements) should be defined ual should f'ollow in parentheses the first time the and introduced parenthetically at first use in the name is used in the text and in the Abstract. Papers text. Enzyme activities should be expressed in the which include extensive taxonomic material (e.g., de- terms set out in CBN's Enzyme Nomenclature (1972) scription of new taxa) will not be published in this (Elsevier Scientific Publishing Co.). Lengths, weights, journal. The proper place for publication of taxonomic volumes, and molarities should make use of the pre- material is the International Journal of Systematic fixes m, A, n, and p (for 10-3, 10-6, 10-9, and 10-12, Bacteriology (IJSB), which is published by the ASM respectively), where applicable, avoiding such com- for the International Association of Microbiological pound prefixes as my and AsM. Metric units should Societies. If the main thrust of such a paper is not tax- be employed as much as possible. The Editors reserve onomy, the paper should be divided, the taxonomy the privilege of editing manuscripts to make them portion being submitted to IJSB and the other portion conform to the above-mentioned stylistic conventions. to this journal. As a rule, all bacterial growth curves should be pre- sented as semilogarithmic plots (log x vs. time). Notes. The accepted form for Notes is somewhat Deviations from this policy are permitted only when different trom the toregoing. Contributors should con- justified in the text. sult a recent issue of the Journal tor style. Notes Form of manuscript. All parts of the manuscript should not exceed 500 words. The Abstract should should be typed double-space or. preferably, triple- not exceed 25 words. space. Most manuscripts can and should be divided Copyright. Once a paper has been published in the into the following sections: Abstract, Introduction, Journal, which is a copyrighted publication, the legal Materials and Methods, Results, Discussion, ownership of all parts of the paper. including the illus- Acknowledgments. and Literature Cited. trations. has passed from the author to the ASM. If Title. A single statement of the subject of a paper is the same author, or any author, wishes to republish preferred to a main title-subtitle arrangement. A short material previously published in the Journal, he version of the title (no more than 46 characters and must first receive written permission from ASM. spaces) should be supplied for use as a running head. First in a New Series VERNITRON's HYDRO-SAN 500 WASHER. Microbilogy YOU SPECIFY ITS FINAL FORM TO BE THE RIGHT 1974/ 'WASHERI FOR YOUR LAB! Now! No need to pay for more than you can use. Or put up with less than you need. TheHydro-San 500 Metal and Glassware Washer is designed specifically for the lab. Handles up to sixteen 6" x 6" (15 Mi|Microbiology.-crobiology -11974974 iss tthehe Tlirstfirst volIvol-- cmx 1 5 cm) basketsof ware at once! 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INDEX TO ADVERTISERS

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* Difco Laboratories .Cover 4 Humko Sheffield Chemical, Div. Kraftco Corp. 8 * Vernitron Medical Products .7

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