Isolation, Identification And
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Desulfuribacillus Alkaliarsenatis Gen. Nov. Sp. Nov., a Deep-Lineage
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central Extremophiles (2012) 16:597–605 DOI 10.1007/s00792-012-0459-7 ORIGINAL PAPER Desulfuribacillus alkaliarsenatis gen. nov. sp. nov., a deep-lineage, obligately anaerobic, dissimilatory sulfur and arsenate-reducing, haloalkaliphilic representative of the order Bacillales from soda lakes D. Y. Sorokin • T. P. Tourova • M. V. Sukhacheva • G. Muyzer Received: 10 February 2012 / Accepted: 3 May 2012 / Published online: 24 May 2012 Ó The Author(s) 2012. This article is published with open access at Springerlink.com Abstract An anaerobic enrichment culture inoculated possible within a pH range from 9 to 10.5 (optimum at pH with a sample of sediments from soda lakes of the Kulunda 10) and a salt concentration at pH 10 from 0.2 to 2 M total Steppe with elemental sulfur as electron acceptor and for- Na? (optimum at 0.6 M). According to the phylogenetic mate as electron donor at pH 10 and moderate salinity analysis, strain AHT28 represents a deep independent inoculated with sediments from soda lakes in Kulunda lineage within the order Bacillales with a maximum of Steppe (Altai, Russia) resulted in the domination of a 90 % 16S rRNA gene similarity to its closest cultured Gram-positive, spore-forming bacterium strain AHT28. representatives. On the basis of its distinct phenotype and The isolate is an obligate anaerobe capable of respiratory phylogeny, the novel haloalkaliphilic anaerobe is suggested growth using elemental sulfur, thiosulfate (incomplete as a new genus and species, Desulfuribacillus alkaliar- T T reduction) and arsenate as electron acceptor with H2, for- senatis (type strain AHT28 = DSM24608 = UNIQEM mate, pyruvate and lactate as electron donor. -
Demonstrating the Potential of Abiotic Stress-Tolerant Jeotgalicoccus Huakuii NBRI 13E for Plant Growth Promotion and Salt Stress Amelioration
Annals of Microbiology (2019) 69:419–434 https://doi.org/10.1007/s13213-018-1428-x ORIGINAL ARTICLE Demonstrating the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration Sankalp Misra1,2 & Vijay Kant Dixit 1 & Shashank Kumar Mishra1,2 & Puneet Singh Chauhan1,2 Received: 10 September 2018 /Accepted: 20 December 2018 /Published online: 2 January 2019 # Università degli studi di Milano 2019 Abstract The present study aimed to demonstrate the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration. NBRI 13E was characterized for abiotic stress tolerance and plant growth-promoting (PGP) attributes under normal and salt stress conditions. Phylogenetic comparison of NBRI 13E was carried out with known species of the same genera based on 16S rRNA gene. Plant growth promotion and rhizosphere colonization studies were determined under greenhouse conditions using maize, tomato, and okra. Field experiment was also performed to assess the ability of NBRI 13E inoculation for improving growth and yield of maize crop in alkaline soil. NBRI 13E demonstrated abiotic stress tolerance and different PGP attributes under in vitro conditions. Phylogenetic and differential physiological analysis revealed considerable differences in NBRI 13E as compared with the reported species for Jeotgalicoccus genus. NBRI 13E colonizes in the rhizosphere of the tested crops, enhances plant growth, and ameliorates salt stress in a greenhouse experiment. Modulation in defense enzymes, chlorophyll, proline, and soluble sugar content in NBRI 13E-inoculated plants leads to mitigate the deleterious effect of salt stress. Furthermore, field evaluation of NBRI 13E inoculation using maize was carried out with recommended 50 and 100% chemical fertilizer controls, which resulted in significant enhancement of all vegetative parameters and total yield as compared to respective controls. -
Thèses Traditionnelles
UNIVERSITÉ D’AIX-MARSEILLE FACULTÉ DE MÉDECINE DE MARSEILLE ECOLE DOCTORALE DES SCIENCES DE LA VIE ET DE LA SANTÉ THÈSE Présentée et publiquement soutenue devant LA FACULTÉ DE MÉDECINE DE MARSEILLE Le 23 Novembre 2017 Par El Hadji SECK Étude de la diversité des procaryotes halophiles du tube digestif par approche de culture Pour obtenir le grade de DOCTORAT d’AIX-MARSEILLE UNIVERSITÉ Spécialité : Pathologie Humaine Membres du Jury de la Thèse : Mr le Professeur Jean-Christophe Lagier Président du jury Mr le Professeur Antoine Andremont Rapporteur Mr le Professeur Raymond Ruimy Rapporteur Mr le Professeur Didier Raoult Directeur de thèse Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR 7278 Directeur : Pr. Didier Raoult 1 Avant-propos : Le format de présentation de cette thèse correspond à une recommandation de la spécialité Maladies Infectieuses et Microbiologie, à l’intérieur du Master des Sciences de la Vie et de la Santé qui dépend de l’Ecole Doctorale des Sciences de la Vie de Marseille. Le candidat est amené à respecter des règles qui lui sont imposées et qui comportent un format de thèse utilisé dans le Nord de l’Europe et qui permet un meilleur rangement que les thèses traditionnelles. Par ailleurs, la partie introduction et bibliographie est remplacée par une revue envoyée dans un journal afin de permettre une évaluation extérieure de la qualité de la revue et de permettre à l’étudiant de commencer le plus tôt possible une bibliographie exhaustive sur le domaine de cette thèse. Par ailleurs, la thèse est présentée sur article publié, accepté ou soumis associé d’un bref commentaire donnant le sens général du travail. -
Exploiting the Aerobic Endospore-Forming Bacterial
Couto et al. BMC Microbiology (2015) 15:240 DOI 10.1186/s12866-015-0575-5 RESEARCH ARTICLE Open Access Exploiting the aerobic endospore-forming bacterial diversity in saline and hypersaline environments for biosurfactant production Camila Rattes de Almeida Couto, Vanessa Marques Alvarez, Joana Montezano Marques, Diogo de Azevedo Jurelevicius and Lucy Seldin* Abstract Background: Biosurfactants are surface-active biomolecules with great applicability in the food, pharmaceutical and oil industries. Endospore-forming bacteria, which survive for long periods in harsh environments, are described as biosurfactant producers. Although the ubiquity of endospore-forming bacteria in saline and hypersaline environments is well known, studies on the diversity of the endospore-forming and biosurfactant-producing bacterial genera/species in these habitats are underrepresented. Methods: In this study, the structure of endospore-forming bacterial communities in sediment/mud samples from Vermelha Lagoon, Massambaba, Dois Rios and Abraão Beaches (saline environments), as well as the Praia Seca salterns (hypersaline environments) was determined via denaturing gradient gel electrophoresis. Bacterial strains were isolated from these environmental samples and further identified using 16S rRNA gene sequencing. Strains presenting emulsification values higher than 30 % were grouped via BOX-PCR, and the culture supernatants of representative strains were subjected to high temperatures and to the presence of up to 20 % NaCl to test their emulsifying activities in these extreme conditions. Mass spectrometry analysis was used to demonstrate the presence of surfactin. Results: A diverse endospore-forming bacterial community was observed in all environments. The 110 bacterial strains isolated from these environmental samples were molecularly identified as belonging to the genera Bacillus, Thalassobacillus, Halobacillus, Paenibacillus, Fictibacillus and Paenisporosarcina. -
Diversity of Culturable Moderately Halophilic and Halotolerant Bacteria in a Marsh and Two Salterns a Protected Ecosystem of Lower Loukkos (Morocco)
African Journal of Microbiology Research Vol. 6(10), pp. 2419-2434, 16 March, 2012 Available online at http://www.academicjournals.org/AJMR DOI: 10.5897/ AJMR-11-1490 ISSN 1996-0808 ©2012 Academic Journals Full Length Research Paper Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco) Imane Berrada1,4, Anne Willems3, Paul De Vos3,5, ElMostafa El fahime6, Jean Swings5, Najib Bendaou4, Marouane Melloul6 and Mohamed Amar1,2* 1Laboratoire de Microbiologie et Biologie Moléculaire, Centre National pour la Recherche Scientifique et Technique- CNRST, Rabat, Morocco. 2Moroccan Coordinated Collections of Micro-organisms/Laboratory of Microbiology and Molecular Biology, Rabat, Morocco. 3Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium. 4Faculté des sciences – Université Mohammed V Agdal, Rabat, Morocco. 5Belgian Coordinated Collections of Micro-organisms/Laboratory of Microbiology of Ghent (BCCM/LMG) Bacteria Collection, Ghent University, Ghent, Belgium. 6Functional Genomic plateform - Unités d'Appui Technique à la Recherche Scientifique, Centre National pour la Recherche Scientifique et Technique- CNRST, Rabat, Morocco. Accepted 29 December, 2011 To study the biodiversity of halophilic bacteria in a protected wetland located in Loukkos (Northwest, Morocco), a total of 124 strains were recovered from sediment samples from a marsh and salterns. 120 isolates (98%) were found to be moderately halophilic bacteria; growing in salt ranges of 0.5 to 20%. Of 124 isolates, 102 were Gram-positive while 22 were Gram negative. All isolates were identified based on 16S rRNA gene phylogenetic analysis and characterized phenotypically and by screening for extracellular hydrolytic enzymes. The Gram-positive isolates were dominated by the genus Bacillus (89%) and the others were assigned to Jeotgalibacillus, Planococcus, Staphylococcus and Thalassobacillus. -
Exploring Bacterial Community Composition in Mediterranean Deep-Sea Sediments and Their Role in Heavy Metal Accumulation
Science of the Total Environment 712 (2020) 135660 Contents lists available at ScienceDirect Science of the Total Environment journal homepage: www.elsevier.com/locate/scitotenv Exploring bacterial community composition in Mediterranean deep-sea sediments and their role in heavy metal accumulation Fadwa Jroundi a,⁎, Francisca Martinez-Ruiz b,MohamedL.Merrouna, María Teresa Gonzalez-Muñoz a a Department of Microbiology, Faculty of Science, University of Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain b Instituto Andaluz de Ciencias de la Tierra (CSIC-UGR), Av. de las Palmeras 4, 18100 (Armilla) Granada, Spain HIGHLIGHTS GRAPHICAL ABSTRACT • The westernmost Mediterranean is highly sensitive to anthropogenic pres- sure. • NGS showed mostly Bacillus and Micro- coccus as dominant in the deep-sea sed- iments. • Culturable bacteria revealed mostly the presence of Firmicutes and Actinobacteria. • Marine culturable bacteria bioaccumulate heavy metals within the cells and/or in EPS. • Lead precipitates in the sediment bacte- rial cells as pyromorphite. article info abstract Article history: The role of microbial processes in bioaccumulation of major and trace elements has been broadly demonstrated. Received 25 September 2019 However, microbial communities from marine sediments have been poorly investigated to this regard. In marine Received in revised form 18 November 2019 environments, particularly under high anthropogenic pressure, heavy metal accumulation increases constantly, Accepted 19 November 2019 which may lead to significant environmental issues. A better knowledge of bacterial diversity and its capability to Available online 22 November 2019 bioaccumulate metals is essential to face environmental quality assessment. The oligotrophic westernmost Med- Editor: Dr. Frederic Coulon iterranean, which is highly sensitive to environmental changes and subjected to increasing anthropogenic pres- sure, was selected for this study. -
Bioprospecting for Novel Halophilic and Halotolerant Sources of Hydrolytic Enzymes in Brackish, Saline and Hypersaline Lakes of Romania
microorganisms Article Bioprospecting for Novel Halophilic and Halotolerant Sources of Hydrolytic Enzymes in Brackish, Saline and Hypersaline Lakes of Romania Robert Ruginescu 1,*, Ioana Gomoiu 1, Octavian Popescu 1,2, Roxana Cojoc 1, Simona Neagu 1, Ioana Lucaci 1, Costin Batrinescu-Moteau 1 and Madalin Enache 1 1 Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy, 296 Splaiul Independentei, P.O. Box 56-53, 060031 Bucharest, Romania; [email protected] (I.G.); [email protected] (O.P.); [email protected] (R.C.); [email protected] (S.N.); [email protected] (I.L.); [email protected] (C.B.-M.); [email protected] (M.E.) 2 Molecular Biology Center, Institute of Interdisciplinary Research in Bio-Nano-Sciences, Babes-Bolyai-University, 42 Treboniu Laurian St., 400271 Cluj-Napoca, Romania * Correspondence: [email protected] Received: 4 November 2020; Accepted: 30 November 2020; Published: 30 November 2020 Abstract: Halophilic and halotolerant microorganisms represent promising sources of salt-tolerant enzymes that could be used in various biotechnological processes where high salt concentrations would otherwise inhibit enzymatic transformations. Considering the current need for more efficient biocatalysts, the present study aimed to explore the microbial diversity of five under- or uninvestigated salty lakes in Romania for novel sources of hydrolytic enzymes. Bacteria, archaea and fungi were obtained by culture-based approaches and screened for the production of six hydrolases (protease, lipase, amylase, cellulase, xylanase and pectinase) using agar plate-based assays. Moreover, the phylogeny of bacterial and archaeal isolates was studied through molecular methods. From a total of 244 microbial isolates, 182 (74.6%) were represented by bacteria, 22 (9%) by archaea, and 40 (16.4%) by fungi. -
Thermolongibacillus Cihan Et Al
Genus Firmicutes/Bacilli/Bacillales/Bacillaceae/ Thermolongibacillus Cihan et al. (2014)VP .......................................................................................................................................................................................... Arzu Coleri Cihan, Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey Kivanc Bilecen and Cumhur Cokmus, Department of Molecular Biology & Genetics, Faculty of Agriculture & Natural Sciences, Konya Food & Agriculture University, Konya, Turkey Ther.mo.lon.gi.ba.cil’lus. Gr. adj. thermos hot; L. adj. Type species: Thermolongibacillus altinsuensis E265T, longus long; L. dim. n. bacillus small rod; N.L. masc. n. DSM 24979T, NCIMB 14850T Cihan et al. (2014)VP. .................................................................................. Thermolongibacillus long thermophilic rod. Thermolongibacillus is a genus in the phylum Fir- Gram-positive, motile rods, occurring singly, in pairs, or micutes,classBacilli, order Bacillales, and the family in long straight or slightly curved chains. Moderate alka- Bacillaceae. There are two species in the genus Thermo- lophile, growing in a pH range of 5.0–11.0; thermophile, longibacillus, T. altinsuensis and T. kozakliensis, isolated growing in a temperature range of 40–70∘C; halophile, from sediment and soil samples in different ther- tolerating up to 5.0% (w/v) NaCl. Catalase-weakly positive, mal hot springs, respectively. Members of this genus chemoorganotroph, grow aerobically, but not under anaer- are thermophilic (40–70∘C), halophilic (0–5.0% obic conditions. Young cells are 0.6–1.1 μm in width and NaCl), alkalophilic (pH 5.0–11.0), endospore form- 3.0–8.0 μm in length; cells in stationary and death phases ing, Gram-positive, aerobic, motile, straight rods. are 0.6–1.2 μm in width and 9.0–35.0 μm in length. -
Isolation of Cultivable Halophilic Bacillus Sp. from the Makgadikgadi Salt Pans in Botswana
® The African Journal of Plant Science and Biotechnology ©2011 Global Science Books Isolation of Cultivable Halophilic Bacillus sp. from the Makgadikgadi Salt Pans in Botswana Bakang Baloi • Maitshwarelo Ignatius Matsheka* • Berhanu Abegaz Gashe Department of Biological Sciences, University of Botswana, Pr Bag UB00704, Gaborone, Botswana Corresponding author : * [email protected] ABSTRACT Halophilic bacteria from the Makgadikgadi salt pans in north central Botswana were isolated using culture-dependent methods. Polymerase chain reaction (PCR) amplification of the 16S rRNA gene and phylogenetic analysis were used to identify the strains. Culturing was done aerobically in six different complex salt media. Salt concentrations used were 15, 20, 25 and 30% (2.6, 3.4, 4.3 and 5.1 M, respectively) NaCl, at pH 7.2 to pH 8.0. Four colony morphology types were isolated in axenic cultures comprising Gram-positive cells. Universal bacterial primers were used to amplify 16S rDNA from chromosomal DNA isolated from three of the four distinct colony groups. Restriction enzyme digests analysis of the 16S rDNA revealed seven RFLP types. Five of the RFLP types were subjected to sequencing. Comparison of the 16S rDNA sequence alignment to reference sequence data bases showed samples S2012A3, S2012B2 and S2012B3 to have between 95 and 99% homology to Bacillus sp. BH 164 and Bacillus sp. HS 136T, a novel species recently described as Bacillus persepolensis. Isolate S4102D4 showed 95 to 99% homology to Thalassobacillus sp. JY0201 and Thalassobacillus sp. FIB228 and Halobacillus sp. MO56 species. All five isolates had at least 95% similarity to published sequences implying they could be species within the described genera. -
Isolation and Diversity of Sediment Bacteria in The
bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Isolation and Diversity of Sediment Bacteria in the 2 Hypersaline Aiding Lake, China 3 4 Tong-Wei Guan, Yi-Jin Lin, Meng-Ying Ou, Ke-Bao Chen 5 6 7 Institute of Microbiology, Xihua University, Chengdu 610039, P. R. China. 8 9 Author for correspondence: 10 Tong-Wei Guan 11 Tel/Fax: +86 028 87720552 12 E-mail: [email protected] 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 29 Abstract A total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using 30 nine different media with 5% or 15% (w/v) NaCl. The number of species and genera of bacteria recovered 31 from the different media significantly varied, indicating the need to optimize the isolation conditions. 32 The results showed an unexpected level of bacterial diversity, with four phyla (Firmicutes, 33 Actinobacteria, Proteobacteria, and Rhodothermaeota), fourteen orders (Actinopolysporales, 34 Alteromonadales, Bacillales, Balneolales, Chromatiales, Glycomycetales, Jiangellales, Micrococcales, 35 Micromonosporales, Oceanospirillales, Pseudonocardiales, Rhizobiales, Streptomycetales, and 36 Streptosporangiales), including 17 families, 41 genera, and 71 species. -
The Occurrence of Potential and Novel Isolates of Oceanobacillus Sp
See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/343877969 The Occurrence of potential and novel isolates of Oceanobacillus sp. JAS12 and Salinicoccus sp. JS20 recovered from West Coast of Arabian Sea, India Article in Research Journal of Biotechnology · September 2020 CITATIONS READS 0 65 3 authors, including: Jayachandra Yaradoddi Agsar Dayanand KLE Technological University, Hubli Gulbarga University 48 PUBLICATIONS 130 CITATIONS 21 PUBLICATIONS 83 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: Bacterial diversity and Biotechnological applications View project Bionanotechnology View project All content following this page was uploaded by Jayachandra Yaradoddi on 26 August 2020. The user has requested enhancement of the downloaded file. Research Journal of Biotechnology Vol. 15 (9) September (2020) Res. J. Biotech The Occurrence of potential and novel isolates of Oceanobacillus sp. JAS12 and Salinicoccus sp. JS20 recovered from West Coast of Arabian Sea, India Yaradoddi Jayachandra S.1,2,3*, Sulochana M.B.2, Kontro Merja H.3, Parameshwar A.B.2 and Agsar Dayanand4 1. Biomaterials Laboratory, Center for Materials Science, KLE Technological University, Hubballi-580031, INDIA 2. Department of Studies and Research in Biotechnology, Gulbarga University, Kalaburagi, Karnataka, 585106, INDIA 3. Ecology and Environmental Research Programme, University of Helsinki, Lahti, FINLAND 4. Department of Studies and Research in Microbiology, Gulbarga University, Kalaburagi, Karnataka, 585106, INDIA *[email protected] Abstract halophilic bacteria are the microbes that can grow under Many halophiles were considered to be extremophiles higher salt concentrations. Halophiles inherently possess due to their inborn industrial potentials and tolerance different industrially important metabolites producing to hostile environmental conditions. -
Reorganising the Order Bacillales Through Phylogenomics
Systematic and Applied Microbiology 42 (2019) 178–189 Contents lists available at ScienceDirect Systematic and Applied Microbiology jou rnal homepage: http://www.elsevier.com/locate/syapm Reorganising the order Bacillales through phylogenomics a,∗ b c Pieter De Maayer , Habibu Aliyu , Don A. Cowan a School of Molecular & Cell Biology, Faculty of Science, University of the Witwatersrand, South Africa b Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology, Germany c Centre for Microbial Ecology and Genomics, University of Pretoria, South Africa a r t i c l e i n f o a b s t r a c t Article history: Bacterial classification at higher taxonomic ranks such as the order and family levels is currently reliant Received 7 August 2018 on phylogenetic analysis of 16S rRNA and the presence of shared phenotypic characteristics. However, Received in revised form these may not be reflective of the true genotypic and phenotypic relationships of taxa. This is evident in 21 September 2018 the order Bacillales, members of which are defined as aerobic, spore-forming and rod-shaped bacteria. Accepted 18 October 2018 However, some taxa are anaerobic, asporogenic and coccoid. 16S rRNA gene phylogeny is also unable to elucidate the taxonomic positions of several families incertae sedis within this order. Whole genome- Keywords: based phylogenetic approaches may provide a more accurate means to resolve higher taxonomic levels. A Bacillales Lactobacillales suite of phylogenomic approaches were applied to re-evaluate the taxonomy of 80 representative taxa of Bacillaceae eight families (and six family incertae sedis taxa) within the order Bacillales.