Organic Framework Nanorod

Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B. This journal is © The Royal Society of Chemistry 2021 Supporting Information Fluorescent Imaging and Precise Suppression of Bacterial Infection in Chronic Wounds by Porphyrin-Based Metal- Organic Framework Nanorod Lihua Zhang a, b, c, Minzhi Ouyang d, Yufei Zhang c, Huanxiang Wang c, Ziyun Huang c, Libei He c, Yanli Lei c, Zhen Zou *c, Feng Feng *a, b, Ronghua Yang *c, e aSchool of Chemistry and Material Science, Shanxi Normal University, Linfen 041004, China bCollege of Chemistry and Environmental Engineering, Shanxi Datong University, Datong 037009, China cHunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410004, China dDepartment ultrasound diagnosis, The Second Xiangya Hospital, Central South University, Changsha, 410011, P. R. China eCollege of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China *Corresponding authors email: [email protected]; [email protected] Lihua Zhang, Minzhi Ouyang and Yufei Zhang contributed equally to this work. S-1 Table of contents Figure S1 The FTIR of TCPP and Zn-TCPP nanorods .............................................S-3 Figure S2 Size distribution and photographs of Zn-TCPP in various media. ...........S-4 Figure S3 MTT assay of Zn-TCPP on MCF-7 and L929 cells..................................S-5 Figure S4 Hemolytic tests of Zn-TCPP with different concentrations......................S-6 Figure S5 Fluorescence images of MCF-7 cells of incubation with different pH.....S-7 Figure S6 Fluorescence images of in vivo with extend chronic wound ....................S-8 Figure S7 Singlet oxygen release in vitro..................................................................S-9 Figure S8 Singlet oxygen release in bacterial..........................................................S-10 Figure S9 Select the sterilization area by controlling the laser. ..............................S-11 Figure S10 H&E and Masson images of the normal tissues on 1st and 8th day. ....S-12 S-2 A TCPP Zn-TCPP . u . a / -1 e 962cm c n -1 a t -1 1683cm t 3310cm i m s n -1 a 997cm r T 3430cm-1 3000 2000 1000 Wavelength/cm-1 Figure S1 The FTIR of TCPP and Zn-TCPP nanorods. S-3 A B 35 30 Water Saline PBS Medium Water 25 Saline PBS Zn-TCPP, 30 h % 20 / e Medium m 15 u l o V 10 5 0 1 10 100 1000 Size/d.nm Figure S2 (A) Size distribution (B) photographs of Zn-TCPP in various aqueous media incubated for 30 h at 37°C. S-4 A B 1.00 MCF-7 1.00 L929 y y 0.75 t t 0.75 i y i l l t i i i l b b i a a i b i V a i 0.50 V l 0.50 l l V e l l C e l e C C 0.25 0.25 0.00 0.00 0 20 40 60 80 0 20 40 60 80 Concentration/mg·mL-1 µ Concentration/mg mL-1 Figure S3 MTT assay of (A) MCF-7 and (B) L929 cells treated with Zn-TCPP nanorods of different concentrations for 24 h. The error bars were the standard deviations from five independent measures. S-5 Concentration of Zn-TCPP(µg·mL-1) (+) (-) 20 50 80 100 200 Figure S4 Hemolytic tests of Zn-TCPP with different concentrations. Ultrapure water and PBS were also used for positive and negative control. S-6 A pH=6 B pH=7 C pH=8 10µm Figure S5 Fluorescence images of MCF-7 cells of incubation with different pH. (A) pH=6, (B) pH=7, (C) pH=8. S-7 A B High 8.4 8.0 H p 7.6 7.2 6.8 Uninfected Infection Low Figure S6 (A) Fluorescence images of in vivo model with extend chronic wound, the circle area was inoculated with 50 µL S. aureus of 1×106 CFU mL−1. (B) The measurement results of pH glass electrodes on different areas of the chronic wound. S-8 B A 1000 15 6min 800 12 . u . a / 600 0 9 y F t / i s F n e 400 0min 6 t n I 200 3 0 0 525 550 575 600 0 1 2 3 4 5 6 Wavelength/nm Time/min Figure S7 (A) Fluorescence spectrum and (B) signal-to-back ratio trend of SOSG fluorescent probe treatment with Zn-TCPP nanorods under illumination of near-infrared light (λex = 660 nm). S-9 Bright field SOSG Merge blank Zn-TCPP Zn-TCPP +Laser 20µm Figure S8 CLSM of SOSG fluorescent probe treatment with Zn-TCPP nanorods under illumination of near-infrared light (λex = 660 nm). S-10 Figure S9 Local photodynamic inactivation of Staphylococcus aureus. The red dotted line range is the illumination range. S-11 PBS Zn-TCPP PBS Zn-TCPP Day 0 Day 8 50µm H&E Masson Figure S10 H&E and Masson images of the normal tissues on the ﬁrst day and eighth day. S-12.

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