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Anti-Human HIST3H3 Polyclonal Antibody Catalog Number: R16071P

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Anti-Human HIST3H3 Polyclonal Antibody Catalog Number: R16071P Rabbit anti-human HIST3H3 Polyclonal Antibody Catalog Number: R16071P Preparation General Information Polyclonal antibody is produced by immunizing rabbit Immunogen A synthetic peptide of human HIST3H3 with a synthetic peptide of human HIST3H3 and purified using protein A resin. IgG type IgG Applications Clonality Polyclonal Western blot Western blot analysis of extracts Specificity human HIST3H3 of various cells, using Histone H3 WB 1:500 - 1:2000 antibody. IHC 1:50 - 1:200 Applications IF 1:50 - 1:200 & dilution IP 1:50 - 1:200 ChIP 1:50 - 1:200 Chromatin Immunoprecipitation PBS with 0.02% sodium azide, 50% Chromatin Formulation glycerol, pH7.3. Immunoprecipitation analysis extracts of HeLa cell line, using Purity ≥95% purity by SDS-PAGE HIST3H3 antibody and rabbit IgG. Store at -20°C. Avoid freeze / thaw Storage cycles. Abbreviation: ELISA: Enzyme-linked immunosorbent assay; ITA: Immunohistochemistry immunoturbidimetric assay; IP: immunoprecipitation; IHC: immuno- Immunohistochemistry histochemistry; IF: immunofluorescence. WB: western blot; FC: of paraffin-embedded flowcytometry rat kidney tissue using Histone H3 antibody Background at dilution of 1:200 Modulation of chromatin structure plays an important role in (x400 lens). the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the Immunohistochemistry primary building block of chromatin. The amino-terminal tails of paraffin-embedded of core histones undergo various post-translational human lung cancer modifications, including acetylation, phosphorylation, tissue using Histone methylation, and ubiquitination. These modifications occur in H3 antibody at dilution response to various stimuli and have a direct effect on the of 1:200 (x400 lens) accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is Immunohistochemistry primarily acetylated at Lys9, 14, 18, 23, 27, and 56. of paraffin-embedded Acetylation of H3 at Lys9 appears to have a dominant role in human liver cancer histone deposition and chromatin assembly in some tissue using Histone H3 antibody at dilution organisms. Phosphorylation at Ser10, Ser28, and Thr11 of of 1:200 (x400 lens) histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunohistochemistry Immunostaining with phospho-specific antibodies in of paraffin-embedded Rat liver using Histone mammalian cells reveals mitotic phosphorylation at Thr3 of H3 antibody at dilution H3 in prophase and its dephosphorylation during anaphase. of 1:100 (x400 lens). For research use only 1 Innovation Drive, Suite 103, Worcester, MA 01605 Tel: 1-508-753-4822 Fax: 1-973-506-2634 E-mail: [email protected] http://www.yurogen.com/ Rabbit anti-human HIST3H3 Polyclonal Antibody Catalog Number: R16071P Immunohistochemistry Immunohistochemistry Immunohistochemistry Immunohistochemistry of paraffin-embedded of paraffin-embedded Human embryo brain Rat testis using using Histone H3 Histone H3 antibody antibody at dilution of at dilution of 1:100 1:100 (x400 lens) (x400 lens). Immunohistochemistry Immunohistochemistry of paraffin-embedded of paraffin-embedded Human mammary rat kidney using gland using Histone HIST3H3 antibody at H3 antibody at dilution dilution of 1:100 (400x of 1:100 (x400 lens) lens) Immunohistochemistry Immunohistochemistry of paraffin-embedded of paraffin-embedded Mouse brain using human liver cancer Histone H3 antibody using HIST3H3 at dilution of 1:100 antibody at dilution of (x400 lens) 1:100 (400x lens). Immunohistochemistry Immunohistochemistry of paraffin-embedded of paraffin-embedded Rat brain using human rectal cancer Histone H3 antibody tissue using Histone at dilution of 1:100 H3 antibody at dilution (x400 lens) of 1:200 (x400 lens) Storage This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20 oC to -70 oC. Avoid freeze/thaw cycles. Hazard/Biohazard This antibody contains 0.02% sodium azide as preservative. Please handle and dispose the product properly. No known biohazard is associated with this For research use only product. 1 Innovation Drive, Suite 103, Worcester, MA 01605 Tel: 1-508-753-4822 Fax: 1-973-506-2634 E-mail: [email protected] http://www.yurogen.com/ .
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