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2 iGEM 2019 - Giant Jamboree iGEM 2019 - Giant Jamboree 3 TABLE OF CONTENTS About ............................................................................................... 6 Contributors ..................................................................................... 7 Sponsors .......................................................................................... 11 Exhibitors ........................................................................................ 12 Maps ............................................................................................... 13 Plaza Level ............................................................................ 13 Halls C and D .......................................................................... 14 Second Floor .......................................................................... 15 Third Floor ............................................................................. 16 Schedule .......................................................................................... 17 Thursday ................................................................................. 18 Friday ..................................................................................... 20 Saturday ................................................................................. 22 Workshops ........................................................................................ 24 Handbook ......................................................................................... 35 4 iGEM 2019 - Giant Jamboree Posters ............................................................................................... 48 Abstracts ............................................................................................. 59 iGEM 2019 - Giant Jamboree 5 ABOUT The International Genetically Engineered Machine (iGEM) Foundation is an independent, non-prof- it organization dedicated to the advancement of synthetic biology, education and competition, and the development of an open community and collaboration. This is done by fostering a cooperative community and friendly competition. The iGEM Foundation’s main programs include: the iGEM Competition - an international com- petition for students interested in the field of synthetic biology; the Labs Program - a program for academic labs to use the same resources as the competition teams; the Registry of Standard Biological Parts - a growing collection of genetic parts for building biological devices and systems; and After iGEM - a community and international network of academics and industry professionals beyond the competition. iGEM began in January 2003 as an independent study course at the Massachusetts Institute of Technology (MIT) where students developed biological devices to make cells blink. This course became a summer competition in 2004 with 5 teams. In 2019, the iGEM Competition has expanded to more than 340 teams from more than 40 countries. The iGEM Competition gives students the opportunity to push the boundaries of synthetic biology by tackling everyday issues facing the world. Made up of undergraduate, graduate, and high school students, multidisciplinary teams work together to design, build, test, and measure a system of their own design using interchangeable biological parts and standard molecular biology techniques. iGEM teams work to create sophisticated projects that strive to make a positive contribution to their local communities and the world. iGEM hosts the Giant Jamboree where all of the iGEM teams come together for four days of in- formation sharing, competition, and celebration of team achievements. During this annual event, iGEM teams present their synthetic biology projects and compete for various awards and prizes. This year, iGEM is proud to host over 340 teams in Boston as they share, celebrate, and showcase their amazing work! 6 iGEM 2019 - Giant Jamboree CONTRIBUTORS iGEM Board of Directors King Chow Richard Johnson Thomas Knight Randy Rettberg Pamela Silver iGEM Foundation Randy Rettberg Meagan Lizarazo President Vice President Maria Bartolini Traci Haddock-Angelli Director of Marketing and Communications Director of the Competition Piers Millet Kitwa Ng Vice President of Safety and Security Assistant Director of Operations Vinoo Selvarajah Ana Sifuentes Director of the Registry Visual Designer and Ambassador to Latin America Abigail Sison Will Wright Laboratory Technician Director of Entrepreneurship iGEM 2019 - Giant Jamboree 7 Executive Judging Committee Peter Carr Director of Judging Beth Beason-Abmayr Janie Brennan Nils-Christian Lübke Jessica C. M. Tang Christopher Workman Patrick Wu Responsible Conduct Committee Peter Carr Chris French Director of Judging Co-Chair King Chow Genya Dana Karmella Haynes Roman Jerala Terry Johnson 8 iGEM 2019 - Giant Jamboree Safety Committee Piers Millet Chair Tessa Alexanian Katherine Littler Thomas Binz Tiago Mendes David Brown Vijayasmitha Moter Brigitte Cadieux Kenneth Oye Peter Carr Megan Palmer Teck Mean Chua Carolina Penalva-Arana Genya Dana Jennifer Robertson Jacqueline van Engelen Larisa Rudenko Sam Weiss Evans Paul Rutten David Gillum Janis Schleusner Anthony Holmes Tim Trevan Chris Isaac Cecile van der Vlugt Furqan Kabir Mathew Watson Alexander Kagansky Nicolas Willemarck Tom Knight Samuel Yu Todd Kuiken Weiwen Zhang Kathleen Lehmann Kathryn Millett iGEM 2019 - Giant Jamboree 9 Human Practices Committee Megan Palmer Todd Kuiken Chair Co-Chair Executive Committee Committee Sam Weiss Evans Marguerite Benony Kevin Chen Hassnain Qasim Bokhari Joy Zhang Ellen Dias Jorgensen Tessa Alexanian Rahmat A. Kemal Pieter van Boheemen Matthew Sample Peter Carr Amy Weissenbach Piers Millett Dorothy Zhang Diversity and Inclusion Committee Anne Meyer Alyssa Henning Chair Co-Chair Christina Agapakis David Llyod Christina Clodomir Darren Nesbeth Alba Iglesias Vilches Peivand Sadat Mousavi Landon Getz V. Anne. Smith Asal Golshaie Brigette Vannier Louise Horsfall Cibele Zolnier S. do Nascimento Divya Israni 10 iGEM 2019 - Giant Jamboree SPONSORS Platinum Partners Partner Sponsors Gold Sponsors iGEM 2019 - Giant Jamboree 11 EXHIBITORS Halls C and D ABSA Human Practices Committee After iGEM Integrated DNA Technologies Agilent Technologies MathWorks Arbor Bioscience Mesurement Committee Batelle New England Biolabs Benchling Opentrons BioBricks Foundation Promega Cornell Alliance for Science Revive and Restore Diversity and Incusion Committee Synlogic FBI Twist Bioscience Feles USDA GenScript Zymergen Ginkgo Bioworks Career Fair Saturday Room 203 1:30-4:30pm Hosted by iGEM As part of the iGEM 2019 Giant Jamboree weekend, iGEM is hosting a career fair event on Satur- day, November 2 to foster relationships within the synthetic biology community. This unique oppor- tunity offers top employers a chance to meet with iGEM participants and discuss career opportuni- ties. Be sure to bring plenty of copies of your resume or CV. Companies: - Twist Bioscience - IDT - GenScript - FBI - Synlogic - Ginkgo Bioworks 12 iGEM 2019 - Giant Jamboree MAPS Plaza Level Access to Prudential Center and Sheraton Hotel Main Entrance on Boylston Street Public Safety Office Business Center First Aid Water Elevator iGEM 2019 - Giant Jamboree 13 Second Level HALL C HALL D AUDITORIUM 207 208 209 210 200 201 202 203 204 205 206 Rooms 202, 204 and 208 Workshops Water Room 203 Career Fair Elevator Room 207 IDT Suite HQ Desk Room 210 Presentation Room Registration 14 iGEM 2019 - Giant Jamboree Halls C and D HALL C HALL D 2 ZONE 2 HS 1 HS 2 ZONE EXHIBITION 1 SPACE ZONE 3 ZONE 1 ZONE Poster Zone 1 1 - 110 Exhibition Space Poster Zone 2 111 - 220 Charging Station Poster Zone 3 221 - 271 Exhibitors Poster HS Zone 1 HS 1 - HS 44 Graffiti Kiosk Poster HS Zone 2 HS 45 - HS 74 iGEM Timeline iGEM 2019 - Giant Jamboree 15 Third Floor BALLROOM C BALLROOM B AUDITORIUM BALLROOM A 310 311 309 312 302 304 306 300 313 301 303 305 307 308 Rooms 302, 304, 306, 309, 310, 311, 312, 313, Ballroom A Presentation Rooms Room 301 Lactation Room Water Room 308 Prayer/Quiet Room Elevator Rooms 303, 305, 307 Extra Seating Gender Neutral Bathrooms HQ Desk 16 iGEM 2019 - Giant Jamboree SCHEDULE Thursday Friday Saturday Sunday Monday October 31 November 1 November 2 November 3 November 4 8:00 Opening Ceremony 8:30 Travel to rooms 9:00 9:30 Presentations Presentations Presentations 10:00 10:30 Break Break Break 11:00 Closing 11:30 Presentations Presentations Presentations Ceremony Auditorium 12:00 12:30 Lunch Lunch 1:00 Lunch 1:30 rkshops rkshops 2:00 rkshops Poster Session Poster Session Poster Session Wo Wo Wo r 2:30 3:00 Presentations Presentations Presentations Career Fai 3:30 4:00 Break Break Break 4:30 5:00 Presentations Presentations Presentations 5:30 Registrarion 6:00 Break Break Break 6:30 7:00 rkshops rkshops rkshops Wo Wo 7:30 Wo Poster Session Poster Session Poster Session iGEM 2019 - Giant Jamboree 17 Friday Start Time Room 210 Room 302 Room 304 Room 306 Room 309 8:00 Opening Ceremony 8:30 Travel to room 9:00 GreatBay SZ Vilnius-Lithuania Bilkent-UNAMBG USAFA CSL Pittsburgh 9:30 XHD-WS-Wuhan-B Marburg NEU CHINA Virginia MADRID UCM HS 10:00 US AFRL CarrollHS uOttawa Jilin China HBUT-China KCL UK 10:30 11:00 Thessaly SYSU-CHINA Tianjin IISc-Bangalore CU 11:30 Aboa CSU Fort Collins NTU-Singapore NAU-CHINA Orleans 12:00 Sriwijaya SDU-Denmark GIFU TOKAI BIT-China UIUC Illinois 12:30 1:00 Poster Session 1:30 Hall C and D Promega Benchling All Friday Teams Workshop Workshop 2:00 Room 202 Room 208 2:30 MITADTBIO Pune DUT China B TAS Taipei