Anti-Lymphotoxin Receptor produced in goat, affinity isolated antibody
Catalog Number L5412
Product Description Precautions and Disclaimer Anti-Lymphotoxin Receptor is produced in goat using This product is for R&D use only, not for drug, as immunogen purified recombinant human household, or other uses. Please consult the Safety lymphotoxin receptor (R) extracellular domain, Data Sheet for information regarding hazards and safe expressed in mouse NSO cells. The antibody is purified handling practices. using human lymphotoxin R affinity chromatography. Preparation Instructions Anti-Lymphotoxin Receptor may be used to localize To one vial of lyophilized powder, add 1 mL of and detect recombinant human lymphotoxin R by 0.2 m-filtered PBS to produce a 0.1 mg/mL stock various immunochemical techniques, including solution of antibody. If aseptic technique is used, no immunoblotting, flow cytometry, and agonist activity. further filtration should be needed for use in cell culture environments. Tumor necrosis factor- (TNF-), also known as lymphotoxin (LT or LT-) and cytotoxic factor, is a Storage/Stability 25 kDa glycoprotein that is tightly regulated by Prior to reconstitution, store at –20 C. Reconstituted lymphocytes. TNF- and TNF- share a 28% amino product may be stored at 2-8 C for up to one month. acid sequence identity and as soluble forms, both can For prolonged storage, freeze in working aliquots at exist in solution as homotrimers, activate the same TNF –20 C. Avoid repeated freezing and thawing. Do not receptors (TNFR1 and TNFR2), and elicit many of the store in a frost-free freezer. same bioactivities. Unlike TNF-, TNF- can also form heterotrimers with one or two molecules of the related Product Profile membrane-bound lymphotoxin- (LT-). Reportedly, Immunoblotting: a working antibody concentration of LT-1/2, the predominant heterotrimer, binds and 0.1-0.2 g/mL is determined. activates only the LT- receptor, LT-R. The other Flow cytometry: a working antibody concentration of heterotrimer, LT-2/1 binds and activates TNFR1 and 2.5 g/106 cells with an appropriate secondary antibody TNFR2, but not LT-R. TNF- is expressed in activated for indirect immunofluorescence staining of cells. T, B, and NK cells and in certain transformed cells.
Genes for TNF-, LT-, and TNF- are tightly linked Agonist activity: this antibody, when immobilized, near each other within the MHC. TNF- appears to play displays agonist activity on HT29 cells in the presence many of the same biological roles as TNF-, but the of 10 U/mL (1 ng/mL) recombinant human IFN-. membrane-bound form with LT- is critical for normal Immobilized antibody at 50 g/mL causes a lymphoid organ development. Genetic polymorphisms 2 to 4.5-fold inhibition of HT29 proliferation. in TNF- and TNF- have been linked to certain pathological conditions, including myasthenia gravis. At Note: In order to obtain the best results in various the amino acid level, human and mouse TNF- are techniques and preparations, it is recommended to 74% homologous and human and mouse LT- are 80% determine optimal working dilutions by titration. homologous. Endotoxin level is 0.1 EU per 1 g antibody as Reagent determined by the LAL method. Lyophilized from 0.2 m-filtered solution in phosphate buffered saline containing carbohydrates.
References 1. Turetskaya, R.L., Polymorphism of the tumor 4. Rennert, P.D. et al., Lymph node genesis is necrosis factor genes: association with disease. induced by signaling through the lymphotoxin Mol. Biol., 33, 355-359 (1999). receptor. Immunity, 9, 71-79 (1998). 2. Ware, C. et al., Tumor necrosis factor-related 5. Browning, J.L. et al., Characterization of ligands and receptors, in The Cytokine Handbook, lymphotoxin-- complexes on the surface of 3rd Edition, Thomson, A.W., (ed.), Academic Press mouse lymphocytes. J. Immunol., 159, 3288-3298 (San Diego, CA: 1998), pp. 549-592. (1997). 3. Zhai, Y.F. et al., Light, a novel ligand for lymphotoxin receptor and tr2/hvem induces SG,PHC,TMS,MAM 06/16-1 apoptosis and suppresses in vivo tumor formation via gene transfer. J. Clin. Invest., 102, 1142-1151 (1998).
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